| [control of dust mites (dermatophagoides pteronyssinus) through the use of a fungicide. experimental observations. clinical trial in children allergic to dermatophagoides]. | | 1985 | 3887534 |
| [study of the purity and specificity of 2 autochthonous allergenic extracts (dermatophagoides pteronyssinus and candida albicans) using the elisa inhibition method]. | | 1985 | 3912948 |
| demonstration of close physicochemical similarity and partial immunochemical identity between the major allergen, dp42, of the house dust mite, d. pteronyssinus and corresponding antigens of d. farinae (df6) and d. microceras (dm6). | house dust mite antigens df6 and dm6, corresponding to dermatophagoides pteronyssinus (dp) antigen 42 (= p1), were purified from cultures of dermatophagoides farinae (df) and dermatophagoides microceras (dm) using hydrophobic interaction chromatography in combination with gel filtration or acetone precipitation. purified antigens from the three mite species (dp42, df6 and dm6) were similar regarding molecular weight by gel filtration (d. pteronyssinus: 17,000, d. farinae: 17,000 and d. microcera ... | 1986 | 3941011 |
| [statistical study done in piedmont on 1100 cases of rhinitis caused by hypersensitivity to inhaled allergens]. | in this study conducted from 1979 to 1984 we have examined 2315 subjects with rhinitis. all the subjects lived in piedmont. the skin tests identified 1100 subjects (47.5%) allergic to at least one of the tested allergens. this group included 575 (52.3%) males and 525 (47.7%) females (males/females = 1.1). in the 6-15 age range we have found a clear predominance of males over females (112 against 57 or males/females = 2). at the time of testing, 854 patients (77.7%) were in the 6-35 age range. on ... | 1986 | 3945416 |
| [cultivation of dermatophagoides pteronyssinus]. | | 1985 | 3990644 |
| [frequency of sensitization to d. pteronyssinus or d. farinae in children with perennial bronchial asthma]. | bronchial sensitization against house dust mites (dermatophagoides farinae, dermatophagoides pteronyssinus) was investigated in 48 children. the therapeutic effect of hyposensitization and removal of house dust mite allergen was evaluated. the diagnosis was established by skin-prick-test, rast and bronchial provocation. the bronchial provocation test was carried out by a whole body plethysmograph with breath by breath registration of the airway resistance. we found a bronchial reaction against b ... | 1985 | 4000137 |
| storage mites as a cause of bronchial asthma in denmark. | from october 1982 to july 1983, 133 consecutive patients complaining of allergic symptoms were prick-tested with three storage mites (acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae) and two housedust mites (dermatophagoides pteronyssinus and dermatophagoides farinae). a total of 60 patients (45%) had a positive prick-test for one or more of the storage mites. a mutual positive correlation was found between the prick-test results for the two house-dust mites as well as for the th ... | 1985 | 4003230 |
| allergy to guinea pigs: i. allergenic activities of extracts derived from the pelt, saliva, urine and other sources. | guinea pig-sensitive patients with asthma and rhinitis were skin test positive to extracts of several materials derived from guinea pigs. a radioallergosorbent test (rast) was developed to measure serum ige specific for the dander, urine, saliva and also for dust from the air-vent filters of a room housing guinea pigs. a strong correlation was found between positive skin test reactions, and raised serum ige to these extracts. furthermore, the relative allergenic potency of extracts was similar w ... | 1985 | 4006174 |
| the occurrence of indoor allergens in saudi arabia. | investigations on indoor airborne allergens in saudi arabia were performed by mold cultures and dust analyses by counter-current immunoelectrophoresis. twenty fungal genera were isolated, with aspergillus as the most often encountered. most of the dust-bound fungi found are ubiquitous and common. antibodies against dermatophagoides pteronyssinus, cat- cow- and rat dander, and cynodon dactylon pollen were used in the dust analyses. animal antigens were found in five of the ten dust samples. house ... | 1985 | 4014781 |
| [importance of animal epithelia as house dust allergens]. | among sera from 379 patients with atopy tested with the radio-allergo-sorbent test (rast) for domestic dust and house-dust mite (dermatophagoides pteronyssinus), 104 were positive only for house dust. positive results for both house dust and house-dust mites were obtained in 130, while in 145 the rast result was positive only for house-dust mites. 104 sera, negative for house-dust mites, were tested by rast for specific ige against epithelia from cats, dogs, guinea-pigs, horses and cattle, as we ... | 1985 | 4017894 |
| pulmonary platelet kinetics in asthma. | platelets produce a range of bronchoconstrictor mediators. measurements of plasma factors have implicated platelet activation in allergic asthma, and sensitised guinea pigs challenged with ovalbumin show pulmonary platelet aggregation accompanying bronchoconstriction. to investigate this further we injected autologous platelets labelled with indium 111 and red cells labelled with technetium 99m into three young volunteers with atopic asthma and three non-asthmatic volunteers and, after equilibra ... | 1985 | 4024000 |
| [evaluation of the immunospecific activity of allergens prepared from house dust and the mite dermatophagoides pteronyssinus in the bacteriosorption reaction on a flat surface]. | the content of protein a-reactive igg to allergens prepared from house dust and d. pteronyssinus was determined in the sera of 4 immunized rabbits, 10 sensitized and hyposensitized guinea pigs and 37 treated and untreated allergic patients by means of the previously developed bacteriosorption test (bst). the sensitivity of the test for the determination of allergen-specific antibodies was 50-100 ng/ml. this test was shown to permit the detection of igg in the sera of immunized, sensitized and hy ... | 1985 | 4072498 |
| serum reagins and leucocyte response in patients with house-dust mite allergy. | a study was carried out on 26 patients with respiratory allergy due to the house-dust mite dermatophagoides pteronyssinus and on 10 non-allergic control subjects using two in-vitro histamine release tests for assessing immediate-type allergy. the first test was on the patients' serum and assessed reaginic (anaphylactic; possibly ige) antibodies by passive sensitization of normal human lung tissue. the second test was on the patients' leucocytes directly challenged with the mite antigen.positive ... | 1970 | 4193661 |
| [epidemiology, diagnosis and treatment of the house-dust-mite allergy in asthmatic children]. | authors introduced as the first in yugoslavia the method of isolation and classifying dermatophagoides pteronyssinus out of house dust using the first the help of spieksma, cunnington and araujo, later on by themselves in new established laboratory. in house dust of 290 houses of asthmatic children in 88 (30,8%) through of cases mites were found, mostly dermatophagoides pteronyssinus. 212 asthmati children who did not react well to specific hyposensitisation with standard house dust extract show ... | 1974 | 4282821 |
| hyposensitization with dermatophagoides pteronyssinus antigen: trial in asthma induced by house dust. | a double-blind clinical trial of hyposensitization with aqueous extracts of dermatophagoides pteronyssinus (the house-dust mite) and human skin scales showed a substantial improvement in symptoms in 11 asthmatics allergic to house dust treated with the d. pteronyssinus extract and a reduction in their need for other therapy. five patients were well for a year but six relapsed. these results contrasted with the generally unfavourable course of the patients treated with the extract of human skin s ... | 1971 | 4940158 |
| house-dust mite asthma. results of challenge tests on five criteria with dermatophagoides pteronyssinus. | twenty-eight patients with asthma and house-dust allergy were investigated by five different challenge tests with mite extract (dermatophagoides pteronyssinus). these tests were performed on the skin, bronchi, nasal mucosa, and leucocytes, and on normal human lung tissue passively sensitized by the patients' serum. the results were compared with each other and with the clinical state of the patients.of 21 patients on whom all five tests were carried out, positive reactions to every test were fou ... | 1970 | 4987898 |
| allergenicity of the house-dust mite dermatophagoides pteronyssinus. | | 1969 | 5792717 |
| [further description of dermatophagoides pteronyssinus (trouessart, 1897). importance of this arachnid in human pathology (psoroptidae: sarcoptiformae)]. | | 1966 | 5931518 |
| [allergenic acari]. | the authors report the milestones which led to the discovery of the mite dermatophagoides pteronyssinus as the main allergen source in house dust, and discuss its etiological role in allergic respiratory diseases. the present knowledge on the importance of other species of mite potentially allergenic in man is focused. from a biological point of view, the taxonomy, anatomy and physiology of these important arthropods are described. furthermore, the techniques for the collection and identificatio ... | 1984 | 6085002 |
| studies on dermatophagoides pteronyssinus allergens: measurement of the relative potencies of d. pteronyssinus purified extracts by in vitro and in vivo methods. | to standardize the dermatophagoides pteronyssinus extracts used in clinical allergy practice, the relative potencies of several purified extracts were estimated by radioallergosorbent test (rast) inhibition, histamine release, and intracutaneous titration testing, and the results were compared. the potencies of nine independently prepared d. pteronyssinus extracts were assayed with respect to an extract adopted as a reference. in vivo the cutaneous wheal surface after intradermal injections was ... | 1980 | 6154726 |
| ascaris lumbricoides and the human immunogenic response: enhanced ige-mediated reactivity to common inhaled allergens. | the relationship between the ige-mediated immunogenic response to ascaris and similar responses to common inhaled allergens was determined. sixty-six of 170 allergic asthmatics from an area in which ascaris infestation was endemic had positive skin-prick reactions to ascaris antigen. the number and size of skin-prick reactions to common inhaled allergens were greater in this group than in the 104 ascaris-negative allergic asthmatics. specific serum ige antibodies were determined by the radio-all ... | 1980 | 6157196 |
| clinical associations with serum allergen-specific igg4 antibodies. | serum total and allergen-specific ige and igg4 antibodies, were measured in eighty-seven atopic and nineteen non-atopic asthmatics. the allergens studied were: dermatophagoides pteronyssinus, grass pollens, cat dander, dog dander, milk and egg. sixty-eight atopic asthmatics and fourteen non-atopic asthmatics were found to have allergen-specific igg4 antibodies, to at least one of the allergens tested. igg4 antibodies to milk and egg were common in both groups of asthmatics, and to animal danders ... | 1982 | 6183023 |
| in vitro selective expansion of allergen specific t cells from atopic patients. | peripheral blood mononuclear cells from atopic donors were stimulated in vitro with allergens (rye group i or dermatophagoides pteronyssinus). t cell lines were originated and maintained in long term culture using il-2 and periodical restimulations with allergen. the lines were antigen specific (i.e. responded to the allergen used to raise them and not to other antigens) and required that the antigen was presented by autologous cells (i.e. they were restricted). the restriction elements were pro ... | 1983 | 6190594 |
| [comparative evaluation of the specific activity of different types of allergens from dermatophagoides pteronyssinus mites]. | the specific activity of different kinds of allergens prepared from dermatophagoid mites has been studied by the method of the indirect mast cell degranulation and the method of changing the volume of cerebral glial cells in patients with the atopic form of bronchial asthma. in cases of sensitization to mites the presence of reagin and precipitin antibodies has been revealed. allergens prepared from the mite medium and directly from mites have been found to possess almost the same specific activ ... | 1983 | 6191478 |
| recognition of two dermatophagoides pteronyssinus-specific epitopes on antigen p1 by using monoclonal antibodies: binding to each epitope can be inhibited by serum from dust mite-allergic patients. | monoclonal antibodies were raised against antigen p1, the major allergen of the house dust mite (dermatophagoides pteronyssinus). the majority were antigen p1 specific, isotype igg1, and did not react with a comparable d. farinae allergen. these antibodies bound 38 to 50% of 125i antigen p1 in antigen-binding assays (titer greater than or equal to 1/1,000,000), and the quantities of igg antibody in ascites were 2 to 4 logs greater than those in polyclonal mouse antiserum or in serum from a mite- ... | 1984 | 6207232 |
| characterization of igg-subclass antibodies to individual allergens by crossed radioimmunoelectrophoresis. | a crossed radioimmunoelectrophoretic (crie) method for detection of human igg subclass specificities against individual antigens is described. after crossed immunoelectrophoresis (cie) of the antigens the immunoplates are incubated with serum, washed and incubated with mouse monoclonal anti-human igg-subclass antibodies. after washing, the plates are finally incubated with the 125i-labelled detector protein, rabbit anti-mouse igg, and washed. the plates are then placed on an x-ray film for autor ... | 1984 | 6335630 |
| in vitro investigations in asthmatic children undergoing hyposensitization with tyrosine-adsorbed dermatophagoides pteronyssinus antigen. | successful hyposensitization to dermatophagoides pteronyssinus in perennial childhood asthma was associated with a slight mean rise of serum igg antibody to the mite antigen in contrast to a placebo group in whom this antibody fell slightly. no relationship was detected between the effect on asthma and the magnitude of this change. nor was there any consistent effect on ige or iga antibody. there was a wide range of total serum ige and ige, igg and iga antibody to d. pteronyssinus before treatme ... | 1984 | 6375891 |
| a new, purified dermatophagoides farinae allergen preparation. evaluation with spt and rast techniques. | a purified, standardized and characterized allergen preparation of the house dust mite dermatophagoides farinae (df) was evaluated by skin prick test (spt) and radioallergosorbent test (rast) in 88 subjects. skin reactions to the purified df preparation and a corresponding crude df preparation were compared. spt was also performed with a crude dermatophagoides pteronyssinus (dpt) preparation to compare reactions obtained with the two mite preparations. by spt the purified df allergen preparation ... | 1984 | 6465481 |
| standardization of allergens. qualitative definition of house dust mite extracts following electroblotting and detection of components with antibody and lectin probes. | components of an extract of dermatophagoides pteronyssinus were electrophoretically separated, transferred to nitrocellulose membranes, and then probed with a variety of antibodies and lectins. using mite-allergic human sera as the first antibody source, ige-binding components were identified. binding patterns by monoclonal and heterologous sera were also obtained. the method is suitable for the qualitative, and ultimately the quantitative, characterization of allergen extracts. the electroblott ... | 1984 | 6500720 |
| antibodies to dermatophagoides pteronyssinus allergen dpt 12 contaminating rabbit antisera to human and mouse anti-immunoglobulins. | antibodies to the allergen dpt 12 from dermatophagoides pteronyssinus have been demonstrated in commercially obtained rabbit anti-human ige, igg and iga antisera and in a commercially obtained rabbit anti-mouse igg1 antiserum using either double or triple antibody radioimmunoassays. where measurable, the titre of anti-dpt 12 antibodies varied both between different anti-isotypes and between batches of the same anti-isotype as judged by the dilution of antiserum required to bind 50% of the radiol ... | 1983 | 6602850 |
| t cell responses to the major allergen from the house dust mite dermatophagoides pteronyssinus, antigen p1: comparison of patients with asthma, atopic dermatitis, and perennial rhinitis. | peripheral blood mononuclear cells (pbmc) from the majority of the allergic patients that we tested who were skin test-sensitive to the house dust mite dermatophagoides pteronyssinus (d. pt.) (46/67) showed significant proliferation in response to the purified major allergen antigen p1; pbmc from 14/15 nonsensitive controls showed no significant response. optimal responses were seen with 10 micrograms antigen p1/ml, but 21 of 43 patients tested showed significant proliferation at 0.01 microgram ... | 1984 | 6609976 |
| antigens and allergens from the common house dust mite dermatophagoides pteronyssinus. part ii. identification of the major ige-binding antigens by crossed radioimmunoelectrophoresis. | crossed immunoelectrophoresis demonstrated 51 antigens in a water-soluble extract of the house dust mite dermatophagoides pteronyssinus. autoradiography demonstrated that 11 of the antigens bound ige antibodies in the sera of house dust mite-allergic patients. ige antibodies in 23 different sera reacted with from one to eight antigens. on the basis of lowenstein's (1978) definition of a "major allergen," two of the antigens, numbers one and 36 would be described as "major allergens." apart from ... | 1984 | 6611362 |
| non-allergenic haemolysins in grass pollens and housedust mites. | 25 batches of pollen (six common grasses, maize, short ragweed) and two batches of housedust mite (dermatophagoides pteronyssinus) all contained haemolysins. the haemolysins of two grass pollens and of the housedust mites were of small mw (100-200 da) and apparently non-allergenic. both caused inflammation when injected into human skin, at doses that could be experienced naturally. the "allergic" airways diseases may not be entirely immunologically mediated. | 1983 | 6638414 |
| effects of diluents on skin tests. | the role of diluents and preservatives on skin tests was examined in 41 allergic patients. the effect of glycerol in intradermal tests was studied. concentrations of five percent glycerol were shown to induce a significant wheal which was not inhibited by clemastine fumarate, suggesting a direct toxic effect without the participation of histamine release. twenty five dermatophagoides pteronyssinus allergic patients were prick tested with standardized and lyophilized extracts reconstituted either ... | 1983 | 6638615 |
| total serum ige levels in venezuelan schoolchildren. | seventy-eight disease-free children were evaluated by prist for total serum ige in order to establish the normal range for this immunoglobulin and assess its utility, in tropical climates, such as venezuela, as a diagnostic tool for clinical allergy. seventy-eight normals were selected from a group of 1053 children, aged 7-12 years from fourteen schools in caracas. exclusion from the normal pool was based on nationality, and on historical, clinical, and/or laboratory evidence of atopic and/or in ... | 1983 | 6640887 |
| [domestic acari in sardinia]. | in sardinia, a great mediterranean island, house dust mites allergy is very spread. this investigation was carried out to determine the species, occurrence and abundance of mites in the house dust of sensitized subjects. 55 samples of house dust were examined and the mites fauna identified. dermatophagoides pteronyssinus was the most abundant (54% of the found mites), and the most frequent mite (94.5%). two others pyroglyphid species were recovered: dermatophagoides farinae and euroglyphus mayne ... | 1983 | 6661294 |
| safety evaluation of thaumatin (talin protein). | thaumatin, the sweet proteinaceous extract of the arils of thaumatococcus daniellii (benth.) has been studied for its subacute toxicity in rats and dogs and its ability to produce anaphylactic antibodies following oral administration to rats and normal human subjects. thaumatin was readily digested prior to absorption in rats and no adverse effects resulted from its continuous administration to rats and dogs at dietary concentrations of 0, 0.3, 1.0 and 3.0% for 13 wk. it was not teratogenic when ... | 1983 | 6686588 |
| skin test reactivity and clinical allergen sensitivity in infancy. | we examined the development of skin test reactivity and clinical allergen sensitivity in infancy. seventy-eight infants of atopic parents were skin prick tested every 4 mo from 4 to 16 mo and an additional 57 of these infants were tested at 20 mo. wheal diameters were recorded for histamine (1 mg/ml) and specific allergen reactions by use of cow's milk, egg albumen, wheat, and dermatophagoides pteronyssinus. the histamine mean wheal diameter was significantly lower at 4 and 8 mo compared to the ... | 1984 | 6699318 |
| [immunochemical and acarological study of mites]. | the data derived in the study of dermatophagoides pteronyssinus by microscopic and immunological assays (double radial immunodiffusion in 2 modifications and indirect rat mast cell degranulation) are provided. the immunochemical assays permitting the detection of mites in 93.3% of cases have been shown to compare very favourably with the microscopic techniques that make it possible to detect mites only in 41.7% of cases. | 1984 | 6704525 |
| the isolation of an allergen from extracts of dermatophagoides pteronyssinus using a murine iga myeloma with a specificity for phosphorylcholine. | the presence of a high molecular weight glycoprotein component bearing the hapten phosphorylcholine (pc) has been demonstrated in some but not all extracts of house dust mite using a phosphorylcholine specific iga myeloma protein (s107). the pc bearing component was isolated from house dust mite extracts by precipitation at equivalence using whole myeloma serum and the isolated fraction was found to be allergenic as judged by a direct rast assay using a highly house dust mite allergic human seru ... | 1980 | 6777077 |
| identification of allergens in dermatophagoides pteronyssinus mite body extract by crossed radioimmunoelectrophoresis with two different rabbit antibody pools. | an extract of purified dermatophagoides pteronyssinus mite bodies was investigated by crossed radioimmunoelectrophoresis (crie), using sera from 29 mite-allergic patients. two crie series consisting of rabbit antibodies to (1) whole mite culture and to (2) purified mite bodies were run simultaneously, showing 27 and 29 precipitates, respectively. cases of major discrepancy between the two series were ascribed to absence of antibody to allergenic components and to artefactual radiostaining due to ... | 1983 | 6828824 |
| comparison of antigenic and allergenic composition of two partially purified extracts from dermatophagoïdes farinae and dermatophagoïdes pteronyssinus mite cultures. | dermatophagoïdes farinae (df 80d) and dermatophagoïdes pteronyssinus (dp 80d) extracts were analyzed for their antigenic and allergenic composition by means of crossed immunoelectrophoresis (cie) and crossed radioimmunoelectrophoresis (crie). by cie, 11 antigens could be numbered in df 80d (df 1 to df 11) and seven antigens in dp 80d (dp 1 to dp 7). this technique allowed us also to define antigens with common as well as specific parts for the two mite species. among the antigens of d. farinae a ... | 1983 | 6853928 |
| characterization of a very high density lipoprotein allergen, dpt 4, from the house dust mite dermatophagoides pteronyssinus. | the allergen dpt 4 from the house dust mite dermatophagoides pteronyssinus was shown to be a lipoprotein as judged by its sudan black b staining properties. ultracentrifugal studies confirmed this finding and showed that dpt 4 was polydisperse, although the majority of the allergen belonged to a very high density lipoprotein class. physicochemical studies using gradient polyacrylamide gel electrophoresis showed that dpt 4 had an apparent molecular weight of 244,000 daltons and a particle diamete ... | 1983 | 6862663 |
| atopic allergy, serum ige and rast specific ige antibodies in patients with cystic fibrosis. | the occurrence of allergic diseases is studied in a group of 56 swiss children suffering from cystic fibrosis (cf). 27% of cf children showed allergic manifestations, and 61% of cf children had positive family history for atopic diseases. the occurrence of allergies is increased in homozygotes as well as in heterozygotes for cf. total serum ige values are clearly increased in atopic cf children, but also slightly elevated in cf patients without allergic manifestations. occurrence of specific ige ... | 1980 | 6967864 |
| allergy to castor bean in the sudan: measurement of serum ige and specific ige antibodies. | allergy to castor bean was diagnosed in thirty-nine dockworkers from port sudan, on the basis of rhinitis and/or asthma provoked by castor bean dust, together with a positive prick test to a high dilution of castor bean extract. these were compared to twelve non-allergic dockworkers from port sudan and forty-three other port sudan residents. the castor bean allergic group had significantly higher ige levels (mean 902 iu/ml) than the non-allergic dock workers (mean 245 iu/ml) or the port sudan re ... | 1981 | 6976240 |
| standardization of allergen extracts by inhibition of rast, skin test, and chemical composition. | five allergen extracts of dermatophagoides pteronyssinus, lolium perenne, alternaria tenuis, aspergillus fumigatus and cladosporium herbarum, obtained from four different manufacturers, were examined by inhibition of rast, content of protein and carbohydrate, contents of phosphorylcholine (pc) and tridacnin reactive components, and by skin test. inhibition of rast was used as a primary method for establishing allergenic potency and demonstrated wide variations for each preparation supplied by th ... | 1980 | 7004662 |
| reproducibility of prick skin tests to five allergens. | thirty-five patients had prick skin tests to the common allergens candida albicans. aspergillus fumigatus, grass pollen, horse dander and dermatophagoides pteronyssinus performed on a regular basis from autumn 1973 to autumn 1975. specific ige to the same allergens (except c. albicans) was determined at the time of skin testing for the first five seasons. it was found that the position on the volar aspect of the forearm on which the test was performed did not affect the reaction. there was a sig ... | 1982 | 7039862 |
| purification of a murine ige-inducing antigen extracted from the house dust mite, dermatophagoides pteronyssinus. | a major antigen inducing ige in mice (dpi) was purified from a crude extract of dermatophagoides pteronyssinus (d.p.) using sephacryl s-200 gel filtration, de52 ion exchange chromatography and isoelectric focusing. this antigen is not one of the major d.p. human allergens. isoelectric focusing showed a single peak of pca activity at pi 4.7. activity followed a broader banding pattern on polyacrylamide gel electrophoresis (page). the molecular weight of dpi estimated from sephadex g-150 chromatog ... | 1982 | 7048069 |
| antigen provocation to the skin, nose and lung, in children with asthma; immediate and dual hypersensitivity reactions. | most (18/21) children with perennial asthma gave dual (immediate and late) responses to bronchial provocation with two of dermatophagoides pteronyssinus, and either timothy grass pollen or cat fur. most (19/21) also showed dual responses to skin prick tests, only half (11/21) gave dual responses in the nose, mainly with timothy grass pollen, and these were associated with allergic rhinitis. only two children gave dual responses in lung, skin and nose to both antigens, and only two gave immediate ... | 1982 | 7083633 |
| evaluation of the intradermal allergy test in normal dogs. | in 45 normal dogs, serial dilutions of different allergens were injected intradermally to determine the skin threshold concentrations. for housedust, housedust mite (dermatophagoides pteronyssinus) and human dandruff extracts these were found to be the same in the dog as in man, while those for animal dandruffs, pollens and fungi were 10 times higher in the dog. the term 'histamine reactive value' (rh) is introduced for the dog. the mean (+/-sd) was found to be 49.0 +/- 2.5 in normal dogs, using ... | 1982 | 7089382 |
| predicting the results of allergen bronchial challenge by simple clinical methods. | in this study, the ability to predict allergenic bronchial reactivity from non-specific bronchial reactivity, measured by free corridor-running, and the level of skin reactivity to whole rye grass pollen extract (wrgpe--lolium perene) and house-dust mite extract (hdm--dermatophagoides pteronyssinus) was examined in asthmatic children. discriminant analysis of the data showed that, for the concentration of extracts used and the conditions of bronchial challenge for both allergens, the level of sk ... | 1982 | 7105393 |
| [correlation between the prick test and specific ige against allergens of house dust and dermatophagoides sp]. | of the techniques which are usually used for the in vitro etiologic diagnosis of determined allergic processes, it is the rast technique which is most widely used for determining specific antibodies of the ige class. on the other hand, also of interest is the quantification of total ige in these patients. we have studied 80 allergic and non allergic patients in order to evaluate the possible correlation between the prick and the rast tests applied to different allergens such house dust. dermatop ... | 1982 | 7180792 |
| [house-dust mites as vectors of human diseases]. | the genuine european house-dust mite, dermatophagoides pteronyssinus, is the producer of the most common and most potent allergen in the house-dust in central europe. such allergens causing asthma, rhinitis, tracheitis, bronchitis and/or conjunctivitis in man may also be produced by the other species of house-dust mites such as d. farinae and euroglyphus maynei. house mites and mites of stored products rarely account for such diseases but more frequently for acarodermatitis--especially on farms. ... | 1982 | 7184173 |
| the relationship between air-borne fungal spores and dermatophagoides pteronyssinus in the house dust. | the prevalence of mould genera and their association with the house dust mite (dermatophagoides pteronyssinus) were investigated in the homes of 192 asthmatic patients and 125 healthy people in a romanian town with a relatively high degree of humidity. the dominant moulds which developed in the culture media exposed on the bedroom floors were cladosporium, penicillium and aspergillus. living dermatophagoides pt. and their eggs were present mostly in the colonies of cladosporium; in those of peni ... | 1981 | 7233050 |
| serum ige before and after treatment for scabies. | in 120 patients, treated for scabies infection, the serum ige concentrations fell from high levels in 54 and normal levels in 66 to lower levels 12 months after treatment. the specific ige antibodies to dermatophagoides pteronyssinus (rast) were also reduced 12 months after treatment. the decrease in both ige concentrations and ige antibodies was most conspicuous in non-atopic patients. these findings confirm previous observations of specific immunological hypersensitivity to scabies mite, and t ... | 1981 | 7235206 |
| the prevalence of immediate positive skin tests in nepalese children. | two hundred and ninety-three normal schoolchildren between 5 and 15 years old living in the hills of eastern nepal were tested by the skin prick method for sensitivity to six allergens: dermatophagoides pteronyssinus, aspergillus fumigatus. cladosporium herbarum, penicillium notatum, mixed pollens and mixed threshings. these children were also questioned and examined for symptoms and signs relating to allergic disease. of the children, 20% were skin-prick positive to at least one of the allergen ... | 1981 | 7249341 |
| correlation between dermatophagoides pteronyssinus and dermatophagoides farinae in house dust mites in chronic urticaria. | | 1981 | 7298373 |
| the distribution of dust mite allergen in the houses of patients with asthma. | using an inhibition radioimmunoassay for the major allergen from dermatophagoides pteronyssinus (antigen p1), we studied the distribution of this dust allergen in the houses of patients with asthma. both bed and floor dust samples contained a wide range of antigen p1, 100 to 100,000 ng/g of fine dust, and this concentration correlated well with the number of mite bodies (r = 0.81, p less than 0.001). we were unable to detect antigen p1 in the air of undisturbed rooms. however, during domestic ac ... | 1981 | 7305119 |
| skin sensitivity reactions in nigerian children with bronchial asthma. | skin sensitivity reactions to a variety of antigens in 290 unselected urban nigerian children suffering from bronchial asthma of varying severity, are reported. the percentages of positive skin reactions to the first four antigens were as follows: ascaris (25%), dermatophagoides pteronyssinus (21%), house dust (12%) and feathers (12%). the sizes of reaction were generally smaller than those reported in european and american children. while the relatively high sensitivity to ascaris was probably ... | 1981 | 7330921 |
| antibody response following prolonged hyposensitization with dermatophagoides pteronyssinus extract. | an antigen-binding radioimmunoassay was used to measure igg, iga and ige antibody (ab) to dermatophagoides pteronyssinus in sera from patients who had been hyposensitized with either aqueous d. pteronyssinus extract or placebo. an allergen, f4p1, was obtained from d. pteronyssinus culture and 125i-labelled for use in the assay. the radioallergosorbent test was also used to measure ige ab to d. pteronyssinus and ige ab to dermatophagoides farinae. the radioimmunoassay results showed a selective r ... | 1980 | 7364496 |
| comparison of cord blood immunoglobulin e concentrations and maternal allergy for the prediction of atopic diseases in infancy. | total serum ige levels were determined in 136 newborns and their mothers and in 54 of their fathers, using the paper radioimmunosorbent test (prist) technique. ige specific antibodies for house dust (dermatophagoides pteronyssinus), orchard grass, timothy grass, and cow's milk were measured with the radioallergosorbent test (rast). one hundred thirty-three rast assays were negative in newborns, and in three cases rast for cow's milk was positive. cord blood ige ranged from 0 to 5.5 iu/ml (mean 0 ... | 1980 | 7372964 |
| human basophil degranulation in dermatophagoides allergies: 93 cases. | in tests conducted on 93 subjects human basophil degranulation (hbd) with dermatophagoides pteronyssinus (dp) and dermatophagoïdes farinae (df), often involved in house dust allergy, were studied. the human basophil degranulation technique involves the enrichment of the circulating basophils of the patient allowing readings in the microscope on a fixed and colored slide and a count of at least 100 basophils, the minimum number necessary to give an adequate interpretation of the results. for each ... | 1980 | 7377643 |
| ige antibodies to house dust mite in patients with scabies. | of 135 patients with scabies, forty-seven (35%) had a positive (classes 1--4) radioallergosorbent test (rast) to dermatophagoides pteronyssinus (dp). elevated serum ige concentrations were found in sixty-one (45%) of the patients. atopic disease was confirmed in twenty-eight (23%) of the 120 patients who completed a medical questionnaire satisfactorily. in a control group, well-matched for age and sex, of 201 employees at a local bank, only four (2%) had a positive rast to dp, and eight (4%) had ... | 1980 | 7426424 |
| allergen skin-prick testing in asthmatic children. | allergen skin-prick tests to the common airborne allergens were performed on 534 children with asthma. a positive reaction to one or more allergens was observed in 81%, and the prevalence increased with age. the specific allergen reactions were characteristic with a high prevalence of reactions to house-dust mite (dermatophagoides pteronyssinus) (88%), house dust (79%, and cat fur (62%), and a low prevalence of grass-pollen reactions (12% to 25%). we found that the diagnosis of atopy could be ma ... | 1980 | 7432312 |
| in vitro demonstration of specific immunological hypersensitivity to scabies mite. | an extract prepared from 200 adult female scabies mites was used for prick and intracutaneous testing in twelve patients with previous scabies infestation, as well as in six healthy controls and three persons with skin sensitivity to dermatophagoides pteronyssinus who had never had scabies before. seven individuals who had had scabies less than a year prior to the testing had positive intracutaneous (immediate type) reactions, whereas all the five who had had scabies more than a year before had ... | 1980 | 7437302 |
| the effect of ascorbate on cellular humoral immunity in asthmatic children. | ten white children with bronchial asthma and exercise-induced bronchoconstriction were assessed immunologically before and 1, 3 and 6 months after the commencement of standard therapy supplemented with ascorbate 1 g/d. the tests of cellular immune function were neutrophil chemotaxis, phagocytosis and resting and stimulated nitroblue tetrazolium reduction, and lymphocyte mitogen-induced transformation. humoral functions measured were secretory iga, serum immunoglobulins, alpha 1-antitrypsin, c3, ... | 1980 | 7444701 |
| screening for ige-mediated allergy. | sera from 425 patients were analysed with phadebas rast reagents against a panel of 19 allergens and the most commonly positive results were to grass pollen, dermatophagoides pteronyssinus and cat epithelium. of the 275 sera rast-positive to any allergen, 261 were positive by a rast technique which used a mixture of the three most common u.k. allergens; the remainder were usually positive to a mould. we conclude that 1) when a positive result is obtained after screening by the mixed-allergen ras ... | 1980 | 7468940 |
| der p i, a major allergen of the house dust mite, proteolytically cleaves the low-affinity receptor for human ige (cd23). | the nature of the proteases that cleave cd23 in vivo is of considerable interest, but remains unknown. here, we demonstrate that der p i, a major allergen of the house dust mite dermatophagoides pteronyssinus, cleaves cd23 from the surface of cultured human b cells (rpmi 8866 b cell line). the cleavage of the receptor from the b cell surface was associated with a parallel increase in soluble cd23 (scd23) in the culture supernatant. furthermore, the proteolytic effect of der p i was specific for ... | 1995 | 7489763 |
| an immunogenetic analysis of the t-cell recognition of the major house dust mite allergen der p 2: identification of high- and low-responder hla-dq alleles and localization of t-cell epitopes. | cellular reactivity to der p 2, a major allergen of the house dust mite (hdm) dermatophagoides pteronyssinus, was studied in a group of 41 symptomatic hdm sensitive patients, using fresh peripheral blood mononuclear cells (pbmc) and assays of proliferation. sixty per cent of the patients responded to der p2, with reactivities being greater in patients with asthma as one of their clinical manifestations and also in those who had skin-test reactivity to a number of allergens. hla-dr and -dq seroty ... | 1995 | 7490115 |
| [prevalence of immediate allergy to respiratory allergens in fodder farmers in doubs]. | the doubs is a damp, semi-mountainous fodder farming department in which occupational respiratory diseases (including asthma) are common in farmers. we studied the prevalence of ige-mediated allergy (total ige, phadiatop and skin prick tests) in a group of 265 exclusive dairy farmers of both sexes of the department and in a control group of non exposed, administrative workers living in the same area. skin prick tests were: dermatophagoides pteronyssinus, acarus siro, cat hair, cow danders, grass ... | 1995 | 7501894 |
| clonal analysis of the atopic immune response to the group 2 allergen of dermatophagoides spp.: identification of hla-dr and -dq restricted t cell epitopes. | the group 2 allergens of dermatophagoides spp. (house dust mite, hdm) are a major immunological target for ige antibodies in the allergic immune response of hdm atopic individuals. in this report the heterogeneity of the t cell repertoire reactive with the group 2 allergen of dermatophagoides pteronyssinus (der p 2) of a hdm allergic individual was investigated using overlapping synthetic peptides. by clonal analysis four distinct t cell epitopes were identified, located within residues 16-31, 2 ... | 1993 | 7508743 |
| skin disease-related t cells bind to endothelial selectins: expression of cutaneous lymphocyte antigen (cla) predicts e-selectin but not p-selectin binding. | cutaneous lymphocyte antigen (cla), defined by the heca-452 antibody, is a cell surface glycoprotein found on a subset of t cells in peripheral blood that binds specifically to e-selectin. this marker is present on the majority of t cells at sites of cutaneous inflammation and immune responses. based upon such evidence, an association between t cell cla expression and skin homing has been proposed. to understand better this relationship, we asked whether putative disease-related, antigen-specifi ... | 1994 | 7517361 |
| [histamine liberation and specific ige against dermatophagoides pteronyssinus in parasitized patients]. | we studied 98 patients with different parasitosis, without clinical symptoms of mite sensitization, most of them coming from guinea. histamine release to dermatophagoides pteronyssinus was performed using whole blood. specific ige to the same antigen was measured by east, as well as by an immunodot with the same antigen extract employed for the histamine release test. finally, the east positive sera were studied by immunoblotting. the presence of specific ige by east could be proved in 31 patien ... | 1994 | 7520207 |
| in vivo biological activity of recombinant der p ii allergen of house-dust mite. | the cdna coding for one of the major allergens of dermatophagoides pteronyssinus, der p ii, has been cloned and expressed in escherichia coli as a fusion protein with glutathione s-transferase. the recombinant material (r der p ii) was tested in the skin of house-dust allergic patients, and was found to be almost as active as the purified native allergen (n der p ii) in stimulating immediate hypersensitivity reactions. the positivity to r der p ii in these patients, who were selected for their s ... | 1994 | 7522071 |
| peptide specificity and hla restriction do not dictate lymphokine production by allergen-specific t-lymphocyte clones. | human and murine cd4+ t lymphocytes can be subdivided into distinct subsets [t-helper type 0 (th0), th1 or th2], based on their lymphokine production profiles. not much is known about the factors that determine these restricted lymphokine secretion profiles. peptide specificity and human leucocyte antigen (hla) restriction may be such factors. as it is well established that allergen-specific t lymphocytes from atopic individuals and non-atopic controls differ in their lymphokine secretion profil ... | 1994 | 7525459 |
| discrepancies between in vitro and in vivo tests for house dust mite allergy: in domestic exposure a better predictor than sensitization? | we subjected seven asthmatic children to two bronchial allergen challenges, first with an extract from the house dust mite dermatophagoides pteronyssinus (der p) and then dermatophagoides farinae (der f), or vice versa. all children had elevated specific serum ige to both species as well as reactions by crossed radioimmuno/electrophoresis (crie) to both group i and ii allergens from both species. immunoabsorption and subsequent analysis by crie showed a considerable concentration of serum ige wi ... | 1994 | 7531110 |
| mechanism of allergic cross-reactions. v. high incidence of unanticipated cross-stimulation by natural allergens of rat basophilic leukemia cells sensitized with monoclonal ige antibodies. | the incidence of cross-stimulations by natural allergens was investigated using rbl-2h3 cells sensitized with five different mouse monoclonal anti-dnp iges and four mercury-induced rat monoclonal iges. cells sensitized with 3 of the 5 monoclonal anti-dnp iges (clones spe-7, srt-1, lb4) responded by serotonin release upon stimulation by natural allergens such as dermatophagoides pteronyssinus, horse dander and mugwort extracts. serotonin release could be inhibited by monovalent dnp-lysine, indica ... | 1995 | 7549509 |
| effect of diuretics on allergen-induced contractions of passively sensitized human bronchi in vitro. | inhaled furosemide has been shown to protect subjects with asthma from bronchoconstriction induced by a wide variety of stimuli, including allergen, but the mechanism of action is controversial. we have used an in vitro model of allergen-induced bronchoconstriction to examine the effects of furosemide and other ion transport inhibitors. human bronchial rings were passively sensitized by incubation with serum from an atopic donor and were challenged with dermatophagoides pteronyssinus. allergen-c ... | 1995 | 7551365 |
| [skin tests for different species of acari in asthmatic children from mexico city]. | house dust mites, dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) are the predominant cause of allergic asthma in our population. to detect the presence of sensitivity to other mites besides dp and df in 100 children that live in mexico city, skin tests were performed in their arms with multi-test devices and glycerinated extracts. there were 34 female and 66 male, aged 4 to 14 years (x = 9.1y), with history of mild and moderate asthma, 2 months to 12 years of evolution. dp ... | 1995 | 7552183 |
| [frequency of allergens in immediate, late, and dual allergic skin response at the national institute of pediatrics]. | we studied 126 children with allergic illness in instituto nacional de pediatria. we applied cutaneous test to determine the early, late and dual response. the results were 68% for the early response, 6% late response and 26% dual response. the allergens more frequently found in the early and late response were dermatophagoides pteronyssinus, dermatophagoides farinae and house dust. approximately the third part of the children studied presented late cutaneous response (dual response more isolate ... | 1995 | 7552188 |
| [prevalence of sensitization to blomia in gran canaria]. | in the present study we assessed the prevalence of sensitizations to blomia in our environment. perennial climatological conditions in the canarian islands facilitates the habitat to mites. the blomia is found in tropical and subtropical homes of europe, usa and asia like the dermatophagoides. we have designed a transversal and prospective study on 100 patients who consulted our unit for the first time. a prick-test for the common allergens, including blomia kulagini, and in vitro determination ... | 1995 | 7572538 |
| quantitation of major allergens in dust samples from urban populations collected in different seasons in two climatic areas of the basque region (spain). | we present the results of allergen content evaluation in 80 dust samples from 31 homes of atopic patients from two climatic areas (humid and subhumid), collected in two seasons of the year (autumn and winter). monoclonal antibody-based immunoassays were used to quantify der p 1, der f 1, der 2, lep d 1, and fel d 1. the results were compared according to climate, season, and the type of sensitization (pyroglyphidae mites, storage mites, or grass pollens). we underline the predominance of dermato ... | 1995 | 7573840 |
| polyinosinic acid: polycytidylic acid promotes t helper type 1-specific immune responses by stimulating macrophage production of interferon-alpha and interleukin-12. | the effects exerted on the development in vitro of dermatophagoides pteronyssinus group i (der p i)-specific t cell lines and t cell clones by addition of polyinosinic acid: polycytidylic acid (poly i:c) in lymphocyte bulk culture were examined. der p i-specific t cell lines generated in presence of poly i:c exhibited reduced ability to produce interleukin (il)-4 and il-5 and developed into der p i-specific cd4+ t cell clones showing a t helper (th) type 0 or th1, instead of th0/th2 cytokine pro ... | 1995 | 7589141 |
| peptide-induced anergy in allergen-specific human th2 cells results in lack of cytokine production and b cell help for ige synthesis. reversal by il-2, not by il-4 or il-13. | the induction of anergy in t cells is believed to be the result of triggering of the tcr in the absence of adequate costimulation mediated through the interaction of cd28 and its ligands, cd80 and cd86. here, we demonstrate that stimulation of human group i allergen in dermatophagoides pteronyssinus extract (der p 1)-specific cd4+ th2-like t cell clones with der p 1-derived peptides in the absence of professional apc results in a state of nonresponsiveness. the induction of anergy occurred despi ... | 1995 | 7594575 |
| a major house dust mite allergen disrupts the immunoglobulin e network by selectively cleaving cd23: innate protection by antiproteases. | asthma is a chronic life-threatening disease of worldwide importance. although allergic asthma and related atopic conditions correlate strongly with immune sensitization to house dust mites, it is unclear why antigens from mites provoke such powerful allergic immune responses. we have characterized the protease activity of der p i, the group i protease allergen of the house dust mite dermatophagoides pteronyssinus, and here report that it cleaves the low-affinity immunoglobulin (ig) e fc recepto ... | 1995 | 7595223 |
| lack of association between hla-dq and -dr genotypes and asthma in southern chinese patients. | asthma is an inflammatory disease with a strong genetic predisposition. we have studied a group of unrelated asthmatic patients of southern chinese origin on their hla-dr and -dq regions using molecular techniques and compared them with 104 healthy controls of the same ethnic origin. restriction fragment length polymorphism (rflp) was used to genotype the mhc class ii dr beta, dq alpha and dq beta loci of the subjects. polymerase chain reaction (pcr) using sequence specific primer (ssp) for dq b ... | 1995 | 7600378 |
| four species of house dust mites recovered from houses of patients with allergic respiratory diseases. | arthropods and their products are wide spread and considered one of the common allergens affecting man. allergy to arthropod antigens is the major cause of respiratory allergy as rhinitis and bronchial asthma. the range of reaction depends on several factors which include the type and source of the material, the rate of intensity, the duration of exposure, the human immune response and history of prior exposure. successful management of allergic disease depends on proper identification of the ca ... | 1995 | 7602163 |
| human ige in severe combined immunodeficiency mice reconstituted with peripheral blood mononuclear cells from dermatophagoides pteronyssinus-sensitive patients. | we studied conditions of human ige formation in severe combined immunodeficiency (scid) mice engrafted with peripheral blood mononuclear cells (pbmcs) from allergic patients sensitive to dermatophagoides pteronyssinus (d.pt.). with 10 x 10(6) pbmcs injected intraperitoneally, the hu-scid mice developed an ige response, but only if experimental animals were immunized with the related allergen. two routes of immunization were tested: intraperitoneal and inhalation. in these experimental conditions ... | 1995 | 7613137 |
| immunochemical characterization of recombinant and native tropomyosins as a new allergen from the house dust mite, dermatophagoides farinae. | two predominant mite species, dermatophagoides farinae and dermatophagoides pteronyssinus, are responsible for the immediate hypersensitivity reaction. although a large number of antigens and allergens was detected in a whole mite culture extract, only the major allergens of der i and ii groups have been extensively studied. much information on other important allergens remains to be accumulated. | 1995 | 7622766 |
| food-induced and occupational asthma due to barley flour. | occupational exposure to inhalant allergens may induce asthma but the presence of asthma after the ingestion of the allergen is rarely reported. | 1995 | 7648375 |
| house dust mite fauna in thailand. | house dust mites have been shown to be the most important source of allergen in thailand. the results of our initial mite survey were in conflict with previous data reported 20 years ago. to evaluate the current condition of mite fauna in thailand, we conducted a larger scale survey of house dust mites in dusts from three different parts of the country. a total of 630 mattress dust samples was collected from various types of housing from central, northern and northeastern parts of thailand. hous ... | 1995 | 7648463 |
| bioassay techniques for evaluating pesticides against dermatophagoides spp. (acari: pyroglyphidae). | three bioassay techniques for testing pesticides against the american house dust mite, dermatophagoides farinae hughes, and the european house dust mite, dermatophagoides pteronyssinus (trouessart), were evaluated using cypermethrin as a standard. all 3 bioassay techniques gave linear responses, and can be used for evaluation of different properties of compounds. the slide dip technique tests for contact activity, the plastic petri dish technique for residual activity on a nonporous surface, and ... | 1995 | 7650713 |
| detection of at least one high-molecular-mass, ige-binding component of the dust mite lepidoglyphus destructor. | we studied the allergen composition in an extract of the storage mite lepidoglyphus destructor. monoclonal antibodies (mabs) were raised against l. destructor by a standard hybridoma technique. in the subsequent screening, we concentrated on mabs fulfilling three criteria. first, in elisa, mabs were assessed against a panel of various mite species, and only those reacting exclusively with l. destructor extract were selected for further analyses. secondly, mabs were selected in immunoblotting acc ... | 1994 | 7653740 |
| optimization and characterization of freeze-dried multilamellar liposomes incorporating different standardized allergen extracts. | desensitization therapy for type i allergy is now current practice. liposomes have been proposed as a support for allergens to improve safety and effectiveness. the aim of this work was to optimize liposomal formulations of three different standardized allergen extracts and to test their allergenicity in vitro and in a preclinical trial. allergen extracts (dactylis glomerata, dermatophagoides pteronyssinus, and cat hair and dander) were associated with multilamellar liposomes of varying composit ... | 1994 | 7653744 |
| a study of the binding of immunoglobulin g and immunoglobulin e from children with bronchial asthma to peptides derived from group ii antigen of dermatophagoides pteronyssinus. | the peptides that were produced by the treatment of the purified group ii antigen of dermatophagoides pteronyssinus with cyanogen bromide, lysylendopeptidase, staphylococcus aureus v8 protease, or n-tosyl-l-phenylalanylchloromethyl ketone-treated trypsin were examined for their binding with igg and ige from children with bronchial asthma by immunoblotting analysis. both igg and ige bound to the peptides from the amino terminus to the 76th residue and the carboxyl terminal side from the 13th or 1 ... | 1993 | 7679489 |
| comparative ige responses to extracts of five species of house dust mite, using western blotting. | ige responses towards extracts of dermatophagoides pteronyssinus, d. farinae, d. microceras, euroglyphus maynei and gymnoglyphus longior have been evaluated with sera from a mite sensitive population and the international reference pool of sera, nibsc 82/528, using western blotting. using mite body and faecal extracts 21 of 29 mite protein bands responded to by sera were common to all five species of mite. all sera were unique with respect to proteins bound and species recognized. mite proteins ... | 1993 | 7680599 |
| comparative analysis of biological activities of der p i-derived peptides on fc epsilon receptor-bearing cells from dermatophagoides pteronyssinus-sensitive patients. | the ability of four uncoupled synthetic peptides (p52-71, p117-133, p176-187, p188-199) derived from der p i, a major allergen from the house dust mite dermatophagoides pteronyssinus (dpt) to stimulate fc epsilon r+ cells from dpt-sensitive patients was comparatively analysed. each free peptide may specifically stimulate basophils (fc epsilon ri+ cells) and platelets (fc epsilon rii+ cells) from patients with significant levels of anti-der p i ige antibodies; p52-71 and p117-133 appear the best ... | 1993 | 7682161 |
| effect of salbutamol, fenoterol, and sodium cromoglycate on the release of heparin from sensitized human lung fragments challenged with dermatophagoides pteronyssinus allergen. | heparin from degranulating mast cells influences a wide range of cellular and humoral reactions associated with allergic inflammation and asthma. agents that inhibit mast cell degranulation may therefore compromise the moderating effects of heparin in the tissues and result in worsening inflammation and other associated pathology. this study measures heparin release from allergen-challenged human lung tissue and compares the effect of the mast cell stabilizing beta 2-agonists, salbutamol and fen ... | 1993 | 7683196 |
| effect of passive sensitization on the mechanical activity of human isolated bronchial smooth muscle induced by substance p, neurokinin a and vip. | 1. the effect of passive sensitization on the mechanical activity of human isolated bronchial smooth muscle induced by the following neuropeptides substance p (sp), neurokinin a (nka) and vasoactive intestinal peptide (vip) was studied both in the absence and in the presence of the neutral endopeptidase (nep) inhibitor, phosphoramidon. 2. cumulative concentration-response curves (ccrc) to these neuropeptides were constructed in human passively sensitized isolated bronchial rings and compared to ... | 1993 | 7684296 |
| t cell epitopes of house dust mite major allergen der p ii. | recent work has indicated the significance of il-4- and il-5-secreting allergen-specific human th2 lymphocytes in the control of immune responses to allergens in atopic individuals. the precise allergenic epitopes that activate these allergen-specific th2 cells are, however, hardly known. we analyzed the epitope-specificity of t lymphocytes specific for der p ii, one of the major allergens of house dust mite dermatophagoides pteronyssinus. using a panel of overlapping synthetic peptides that spa ... | 1993 | 7688399 |