| immunoglobulin genes, hla-b8/dr3, and immune responsiveness to primary immunogen and mitogens in normal subjects. | the genetic regulation of immune responsiveness by genes from two independent, highly polymorphic genetic systems, namely immunoglobulin allotypes and human leukocyte antigens (hla), was studied in 35 healthy caucasian volunteers. the in vivo igg class antibody response to the primary test immunogen alpha-helix pomatia hemocyanin (hph) was increased in subjects with the gm1,17;..;21 haplotype compared to that of the non-gm1,17;..;21 group. the igm-class response tended to be higher in the former ... | 1988 | 3259481 |
| inhibition of phosphatase and sulfatase by transition-state analogues. | the inhibition constants for vanadate, chromate, molybdate, and tungstate have been determined with escherichia coli alkaline phosphatase, potato acid phosphatase, and helix pomatia aryl sulfatase. vanadate was a potent inhibitor of all three enzymes. inhibition of both phosphatases followed the order wo4(2-) greater than moo4(2-) greater than cro4(2-). the ki values for potato acid phosphatase were about 3 orders of magnitude lower than those for alkaline phosphatase. aryl sulfatase followed th ... | 1988 | 3280015 |
| detection of ocular mucus in normal human conjunctiva and conjunctiva from patients with cicatricial pemphigoid using lectin probes and histochemical techniques. | conjunctival biopsies from patients with cicatricial pemphigoid affecting the conjunctiva and patients undergoing cataract surgery (normal conjunctiva) were snap-frozen, cryostat sectioned and incubated with fluorescein-conjugated lectins; peanut agglutinin (pna), helix pomatia agglutinin (hpa), soybean agglutinin (sba), wheat germ agglutinin (wga) and succinylated wheat germ agglutinin (s-wga). controls consisted of preincubating the lectins with the appropriate blocking sugars before applying ... | 1988 | 3289955 |
| helix pomatia and ulex europeus lectin binding in human breast carcinoma. | formalin fixed, paraffin embedded tissue from 100 consecutive cases of breast carcinoma were studied for binding with helix pomatia (hpa) and ulex europeus (uea1) lectins. serial sections were pretreated with trypsin or neuraminidase to determine the effect of these enzymes on lectin binding. the lectins were visualized by the peroxidase antiperoxidase technique and the cell staining proportion assessed in a semi-quantitative manner under the light microscope. correlating staining with prognosti ... | 1987 | 3309230 |
| the subcellular localization of apomucin and nonreducing terminal n-acetylgalactosamine in porcine submaxillary glands. | antibodies prepared against enzymatically deglycosylated porcine submaxillary gland mucin (apomucin), which were unreactive with native mucin and its partially deglycosylated derivatives, were used to immunolocalize apomucin in situ. electron microscopy of sections of lowicryl k4m-embedded tissue reacted successively with antibodies and protein a-gold complexes showed apomucin exclusively in mucous cells within the rough endoplasmic reticulum, transitional elements of the endoplasmic reticulum, ... | 1988 | 3339018 |
| cytochemical localization of blood group substances in human salivary glands using lectin-gold complexes. | we investigated localization of blood group antigens and their related substances in human labial salivary and submandibular glands by application of a post-embedding cytochemical staining procedure using lectin- or glycoprotein-gold complexes. surgical tissue was obtained from 10 patients. blood group-specific lectins, such as dolichos biflorus agglutinin or helix pomatia agglutinin (group a-specific), griffonia simplicifolia agglutinin-i b4 (group b-specific), and ulex europaeus agglutinin i ( ... | 1988 | 3346537 |
| ultrastructural studies of glycoconjugates in brain micro-blood vessels and amyloid plaques of scrapie-infected mice. | lectin or glycoprotein-gold complexes and samples of scrapie-infected mouse brain embedded in lowicryl k4m were used for ultrastructural localization of glycoconjugates. the lectins tested recognize the following residues: beta-d-galactosyl [rca, ricinus communis agglutinin (aggl.) 120], n-acetyl and n-glycolyl neuraminic acid (lfa, limax flavus aggl.), n-acetyl-d-glucosaminyl and sialyl (wga, wheat germ aggl.), n-acetyl-d-galactosaminyl (hpa, helix pomatia aggl., and dba, dolichos biflorus aggl ... | 1988 | 3348084 |
| lectin-gold labeling of glycoconjugates in normal and brattleboro rat papilla: effect of vasopressin. | some reports suggest that the plasma membrane glycocalyx of collecting duct epithelial cells, as well as interstitial glycoconjugates, may be involved in vasopressin action and urinary concentration. in view of this, we have used the lectin-gold technique to map and quantify helix pomatia lectin (hpl)-binding sites in the inner medulla of kidneys from normal long-evans rats, vasopressin-deficient brattleboro rats, and brattleboro rats treated for up to 5 wk with exogenous vasopressin. the result ... | 1988 | 3348385 |
| glycolipid-lectin interactions: reactivity of lectins from helix pomatia, wisteria floribunda, and dolichos biflorus with glycolipids containing n-acetylgalactosamine. | the autoradiographic detection of 125i-labeled lectins binding to glycolipids on thin-layer chromatograms can be used to rapidly analyze total glycolipid extracts of cells or tissues for specific oligosaccharide structures. the helix pomatia lectin which binds with high affinity to terminal alpha-linked galnac residues did not bind to globoside (terminal beta 1-3galnac) but did bind the ganglioside gm2 and its asialo derivative which have terminal beta 1-4galnac residues. the lectin from dolicho ... | 1988 | 3355161 |
| biosynthesis of the human sucrase-isomaltase complex. differential o-glycosylation of the sucrase subunit correlates with its position within the enzyme complex. | the biosynthesis and maturation of human sucrase-isomaltase (si, ec 3.2.1.48-10), was studied in cultured small intestinal biopsy specimens and mucosa explants. pulse-chase experiments with [35s]methionine revealed one high mannose intermediate of mr = 210,000 (pro-sih) which was processed at a slow rate to an endo h-resistant, mature form of mr = 245,000 (pro-sic). the fully core-glycosylated form (mr = 212,000) was detected only when 1-deoxynojirimycin was added to the culture medium, thus ind ... | 1988 | 3366777 |
| purification of forssman and human blood group a glycolipids by affinity chromatography on immobilized helix pomatia lectin. | a method for the affinity purification of intact glycolipids having nonreducing terminal alpha 1-3 linked n-acetylgalatosamine residues has been developed. this technique relies on the retention of the carbohydrate-binding specificity of immobilized helix pomatia lectin in aqueous solutions of tetrahydrofuran. both forssman glycolipid and a mouse blood group a-active hexaosylceramide were bound by columns of the lectin equilibrated in a solvent containing 95% tetrahydrofuran and 5% water. after ... | 1988 | 3389512 |
| identification of ecdysone 25-o-beta-d-glucopyranoside as a new metabolite of ecdysone in the nematode parascaris equorum. | a major metabolite of [3h]ecdysone injected into adults of the nematode parascaris equorum maintained in vitro for 48 h was secreted into the culture medium. the compound could be hydrolysed with a crude hydrolase preparation from helix pomatia, yielding ecdysone. sufficient quantity of this material for identification was produced by administration of ecdysone to the parasites. the resulting compound was purified by h.p.l.c. and identified as ecdysone 25-o-beta-d-glucopyranoside by n.m.r. spect ... | 1987 | 3435447 |
| ultrastructural localization of helix pomatia lectin-binding sites in mouse lung elastic fibers. | helix pomatia (snail) lectin complexed with colloidal gold (hpl-gold) recognized binding sites on elastic fibers in plastic embedded sections of lung tissue from mice of several ages. deposition of the lectin-gold particles was examined by electron microscopy. structures such as the elastic laminae of pulmonary vessels and elastic fibers throughout the lung was specifically and intensely decorated by the hpl-gold complex and easily visualized. the binding of the hpl-gold particles was primarily ... | 1987 | 3436842 |
| characterization of the soluble immune complexes that are detected by three different techniques. | three different tests which are based on different principles were used for the detection of soluble immune complexes (ic): (i) a peg precipitation test, which is based on the solubility characteristics of ic; (ii) a solid-phase c1q binding assay, which is based on the complement binding property of ic, and uses peroxidase-linked anti-human igg to detect the bound ic (c1q-elisa); and (iii) the indirect granulocyte phagocytosis test (igpt) which is based on the fc r- and possibly the c3 r-binding ... | 1986 | 3484441 |
| the carbohydrate moieties of suppressor igg-binding factor released by murine t cells. | the carbohydrate moieties of murine igg-binding factor (igg-bf) were studied using lectins binding n-glycosylated sequences such as concanavalin a (con a), lens culinaris agglutinin (lca), and wheat germ agglutinin (wga), and lectins binding o-glycosylated sequences such as peanut agglutinin (pna) and helix pomatia agglutinin (hpa). sources of igg-bf were: (1) supernatants from t2d4, a t cell hybridoma constitutively producing igg-bf, and (2) factor purified by affinity chromatography on rabbit ... | 1987 | 3500399 |
| fluorochrome-coupled lectins reveal distinct cellular domains in human epidermis. | the distribution of saccharide moieties in human interfollicular epidermis was studied with fluorochrome-coupled lectins. in frozen sections concanavalin a (con a), lens culinaris agglutinin (lca), ricinus communis agglutinin i (rcai), and wheat germ agglutinin (wga) stained intensively both dermis and viable epidermal cell layers, whereas peanut agglutinin (pna) bound only to living epidermal cell layers. ulex europaeus agglutinin i (ueai) bound to dermal endothelial cells and upper cell layers ... | 1986 | 3512692 |
| biochemical properties of larval excretory-secretory glycoproteins of the parasitic nematode toxocara canis. | toxocara canis larvae, infective to man, secrete a number of antigenic macromolecules into culture medium over prolonged periods of time. these antigens have been collected and characterised with respect to molecular weight by sodium dodecyl sulphate-polyacrylamide gel electrophoresis following extrinsic and intrinsic radiolabelling, or electrophoresis followed by gel staining or immunoblotting. panels of enzymes and lectins have been applied to examine protease sensitivity and carbohydrate comp ... | 1986 | 3515176 |
| cytotoxic activity in the coelomic fluid of the annelid eisenia foetida sav. | the annelid eisenia foetida not only causes hemolysis of red blood cells of several vertebrate species, but also has a toxic effect on a variety of cell types, such as chicken fibroblasts, guinea-pig polymorphonuclear leukocytes and insect hemocytes. however, it has no influence on the vitality of the coelomocytes of lumbricus terrestris and other lumbricides, nor on the hemocytes of the snail helix pomatia, the mussels anodonta cygnea and unio tumidus, free cells of the turbellarian euplanaria ... | 1987 | 3571568 |
| the golgi apparatus in the acinar cells of the developing embryonic pancreas: ii. localization of lectin-binding sites. | the reaction patterns of the golgi apparatus following staining with the lectins concanavalin a (cona), ricinus communis i agglutinin (rca i), and helix pomatia lectin (hpa) were studied in the pancreas acinar cells of rat embryos in the course of cell differentiation from day 13 through day 20 of gestation. the binding reactions were localized by means of pre-embedment incubation of 10-microns-thick cryosections of pancreas tissue, prefixed in a mixture of 4% formaldehyde/0.5% glutaraldehyde, u ... | 1987 | 3578086 |
| litomosoides carinii: characterization of surface carbohydrates of microfilariae and infective larvae. | the carbohydrate moieties of microfilariae (mf) and infective larvae (l3) have been investigated by lectin-binding technique. mf derived from three sources, namely, uteri (in utero), released in vitro from adults and from blood of rodents infected with litomosoides carinii were examined by using fluoresceinated lectins. wheat germ agglutinin (wga) bound to these mf and the binding was inhibited by n-acetyl glucosamine. in addition to wga, concanavalin a (con a) and lentil lectin (lch) bound to i ... | 1987 | 3602835 |
| complete amino-acid sequence of a functional unit from a molluscan hemocyanin (helix pomatia). | from the beta c-hemocyanin (beta c-hc) of the vineyard snail, helix pomatia, the functional unit d (mr approximately equal to 50,000-55,000) was isolated by limited proteolysis and gel chromatography. a small quantity of functional unit d was obtained intact, but the major part in the form of two peptides (mr approximately equal to 43,000 and 10,000, respectively) connected by a disulfide bridge. after reduction and carboxymethylation, these were separated from each other and cleaved by conventi ... | 1987 | 3620107 |
| analysis of urinary aldosterone metabolites in the guinea-pig. | analysis of urinary metabolites of [1, 2-3h]-aldosterone was performed in the male guinea-pig. separation of urinary metabolites was carried out by countercurrent distribution followed by deae-sephadex a-25 column chromatography. a major component was obtained which was both hydrolyzable with sulphatase from helix pomatia and solvolyzable. paper chromatography of freed steroids revealed the presence of at least two components and the major aglycone cochromatographed with 3 beta, 5 alpha-tetrahyd ... | 1987 | 3622385 |
| echinococcus granulosus: occurrence of ecdysteroids in protoscoleces and hydatid cyst fluid. | both free ecdysteroids and hydrolysable polar conjugated ecdysteroids were detected in protoscoleces of echinococcus granulosus from the equine host, and in hydatid cyst fluid from the same source. comparisons were made of hydatid cyst fluid from e. granulosus infections of three intermediate host species: horses, sheep and humans. ecdysone and 20-hydroxyecdysone were identified in both protoscoleces and hydatid cyst fluids by high-performance liquid chromatography monitoring fractions by radioi ... | 1987 | 3627169 |
| differences in glycocalyx composition between cells of the cortical thick ascending limb of henle and the macula densa revealed by lectin-gold cytochemistry. | the glycocalyx composition of cells from the macula densa and the cortical thick ascending limb of henle was examined in rabbit kidney by means of the lectin-gold technique. a quantitative evaluation at the ultrastructural level showed that macula densa cells had a considerably greater affinity for helix pomatia lectin than adjacent cells of the thick ascending limb. wheat germ lectin and concanavalin a bound equally well to both cell types. this difference in plasma membrane glycocalyx composit ... | 1987 | 3631539 |
| neurite-promoting activity from fetal skeletal muscle: partial purification of a high-molecular-weight form. | neurite extension from sensory neuroblasts dissociated from chick embryo dorsal root ganglia can be stimulated by precoating the polylysine culture surface with extracts of skeletal muscle from bovine fetuses. the active factor(s) may be partially purified from cytosolic extracts of muscle by chromatography on sepharose 6b and affinity chromatography on wheat germ agglutinin or helix pomatia agglutinin columns. extract concentrations of 10-50 micrograms protein per 1 ml were active in promoting ... | 1986 | 3701885 |
| [changes in the chemosensitive properties of the central neurons of the snail helix pomatia and of the mouse as affected by opioid peptides]. | in experiments on neurones of the isolated ganglia of the snail and surviving hippocampal slices from mice, studies have been made of the effect of met- and leu-enkephalins on neuronal sensitivity to acetylcholine and norepinephrine. it was shown that in some cases the enkephalins do not affect bioelectrical activity of the neurones, but changed their responses to acetylcholine and norepinephrine. it is concluded that during investigation of the effect of enkephalins on the nervous cells, alongs ... | 1986 | 3716685 |
| insulin-like growth factor-binding protein from human plasma. purification and characterization. | insulin-like growth factor (igf)-binding protein (bp) has been purified from cohn fraction iv of human plasma by acidification, ion exchange to remove endogenous ligands, and affinity chromatography on agarose-igf-ii. the pure protein appeared as a single peak by high performance reverse-phase and gel permeation chromatography (molecular mass, 45-50 kda), but on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gave a major band at 53 kda and a minor band at 47 kda, unreduced, or 43 and ... | 1986 | 3722172 |
| [effect of enkephalins on central neuronal activity in the snail helix pomatia and the white mouse]. | | 1986 | 3739467 |
| changes in the distribution of endothelial surface glycoconjugates associated with altered permeability of brain micro-blood vessels. | lectin-binding sites located on the endothelial cell (ec) surfaces in unaltered, leaking and resorbing micro-blood vessels (mbvs) in cryo-injured cat brain were studied. lectin or glycoprotein-gold complexes and brain samples embedded in hydrophilic resin lowicryl k4m were used. the lectins tested recognize the following residues: beta-d-galactosyl (ricinus communis agglutinin 120, rca and peanut agglutinin, pna), sialyl (limax flavus agglutinin), n-acetyl-d-galactosaminyl (helix pomatia aggluti ... | 1986 | 3739620 |
| histochemistry of glycosaminoglycans in cartilage ground substance. alcian-blue staining and lectin-binding affinities in semithin epon sections. | the critical-electrolyte-concentration staining method using alcian blue (ab) was applied to etched semithin epon-embedded sections. the distribution of various glycosaminoglycans (gags) was studied in hyaline, elastic, cellular and fibrous cartilage obtained from humans and rodents. the staining patterns in semithin sections were found to correspond to those obtained using paraffin-embedded material. lectin histochemistry was performed on consecutive sections. the following peroxidase-labelled ... | 1986 | 3744895 |
| ultrastructural localization of lectin receptors on the luminal and abluminal aspects of brain micro-blood vessels. | lectin- or glycoprotein-colloidal gold complexes were used for detection of specific monosaccharide residues in mouse brain micro-blood vessels (mbvs). the lectins tested recognize the following residues: beta-d-galactosyl (ricinus communis agglutinin-120, rca-1), alpha-n-acetylgalactosaminyl (helix pomatia agglutinin, hpa), alpha-d-mannosyl and alpha-d-glucosyl (concanavalin a, con a), sialoglycoconjugates (limax flavus agglutinin, lfa), n-acetylglucosaminyl and sialyl (wheat germ agglutinin, w ... | 1986 | 3753715 |
| lectin-dependent recognition of foreign cells by hemocytes of the mussel, mytilus edulis. | phagocytosis of human erythrocytes (rbc) by hemocytes of the mussel mytilus edulis was found to be influenced by four heterologous lectins. the effects were examined in the absence of ca++ ions under three experimental conditions: when the lectins were bound to 1) both hemocytes and rbc, 2) only hemocytes, but not to rbc, and 3) only rbc, but not to hemocytes. the lectins used included: albumen gland agglutinin from helix pomatia (hpa), wheat germ agglutinin (wga), ricinus-120 (ric-120) and conc ... | 1986 | 3754847 |
| characterization of surface glycoproteins on schistosoma mansoni adult worms by lectin affinity chromatography. | adult schistosoma mansoni were radiolabeled by direct radioiodination using the bolton-hunter reagent or by metabolic labeling using radioactive hexose precursors. tegumental material was extracted by freeze-thaw or by incubation in the non-ionic detergent nonidet p-40, then applied to chromatography columns containing the following immobilized lectins: con a, lentil lectin, wheat germ agglutinin, soybean agglutinin and the agglutinins from ricinus communis and helix pomatia. sds-page analysis o ... | 1986 | 3755470 |
| hydrolysis of conjugated steroids by beta-glucuronidase from ampullaria and application to the determination of urinary 17-hydroxycorticosteroids. | the usefulness of ampullaria beta-glucuronidase as a reagent for the hydrolysis of steroid glucuronides was examined. the purified enzyme showed an optimum ph of 4, and was stable between ph 4.5 and 7.5. the enzyme from ampullaria and helix pomatia hydrolyzed various artificial substrates and conjugated steroids. affinity of the ampullaria enzyme for 17-hydroxycorticosteroid glucuronide was 7-fold the affinity of the helix pomatia enzyme. hydrolysis of urinary steroid conjugates was completed by ... | 1986 | 3791634 |
| glycosylation of developing human glomeruli: lectin binding sites during cell induction and maturation. | expression of cellular glycoconjugates during differentiation of human fetal kidney was studied using fluorochrome-labeled lectins. each lectin revealed a characteristic binding pattern during the phenotypic change of the nephrogenic mesenchyme and during distinct stages of nephron development. the uninduced mesenchymal cells were positive for pisum sativum (psa), concanavalin a (cona), wistaria floribunda (wga), and ricinus communis (rca-i) lectins. however, these lectins failed to react with t ... | 1987 | 3794308 |
| lectin histochemistry of galactose and n-acetyl-galactosamine glycoconjugates in normal gastric mucosa and gastric cancer and the relationship with abo and secretor status. | the histochemical binding of four lectin-peroxidase conjugates to normal human gastric mucosa and gastric carcinoma is described. the lectins were peanut agglutinin (pna) which is specific for galactose residues and soy bean agglutinin (sba), dolichos biflorus agglutinin (dba) and helix pomatia agglutinin (hpa) which are specific for n-acetylgalactosamine. binding of pna to surface mucous cells or normal gastric mucosa occurred in non-secretors but not secretors and was independent of abo blood ... | 1986 | 3794866 |
| glycoconjugate cytochemistry of the rat small intestine using helix pomatia agglutinin and colloidal gold conjugates. | | 1986 | 3805962 |
| preparation of protein colloidal gold complexes in the presence of commonly used buffers. | in the presence of various commonly used buffers, phosphate-buffered saline (pbs), tris-buffered saline (tbs), na-cacodylate, bovine serum albumin and a wide range of cytochemically active proteins (monoclonal and polyclonal igg, concanavalin a, ricinus communis lectin i, helix pomatia lectin, protein a) were complexed to colloidal gold of different particle sizes (6 nm, 9 nm, 22 nm). the resulting complexes were active in cytochemical labelling. complex-formation in the presence of electrolyte ... | 1986 | 3816820 |
| lectin-binding to hemocytes of mytilus edulis. | lectin receptors for wga, ricinus 60, ricinus 120, and for the agglutinin from the albumin gland of helix pomatia were detected on the surface of mytilus hemocytes by an agglutination assay. when hemocyte monolayers on slides were incubated with these lectins, strong rosette formation was obtained after addition of human erythrocytes. the lowest lectin concentration which caused formation of rosettes was determined to be 5 micrograms/ml of wga or of helix agglutinin, and 20 micrograms/ml of rici ... | 1985 | 3839466 |
| phylogenetic aspects of cardiac hormones as revealed by immunocytochemistry, electronmicroscopy, and bioassay. | a hormone family of cardiac peptides has recently been isolated and biochemically and pharmacologically characterized by the relaxation of vascular smooth muscle, diuretic and natriuretic activities. the cardiac hormones are stored in specific granules of the atrial myoendocrine cells. since data is available only from mammals (rat, pig, man) we started a phylogenetic study by investigating representatives of the higher vertebrate classes (birds, reptiles, amphibians, bony fish) as well as an in ... | 1985 | 3841692 |
| [lectin-inducible cell cytotoxicity and the production of the cytotoxic factor of natural killer cells during immobilization stress in mice]. | mice exposed to immobilization stress manifested a dramatic lowering of the activity of normal killers against yac-1 tumor as well as against the lectin-induced cellular cytotoxicity mediated by helix pomatia agglutinin and detected by the cytolytic test against xenogeneic tumor cells k 562. exposure to stress did not provoke any change in the production of cytotoxic factor of normal killers, whereas dexamethasone (5 x 10(-7) m) in vitro inhibited the production of this factor. | 1985 | 3860263 |
| biosynthesis of the major human red cell sialoglycoprotein, glycophorin a. o-glycosylation. | the biosynthesis of the major human red cell sialoglycoprotein, glycophorin a, was studied in the erythroleukemia cell line k562 with emphasis on o-glycosylation. the cells were pulse-chase labeled with [35s] methionine, and either directly immune precipitated with anti-glycophorin a antiserum or detergent-solubilized extracts first passed through columns containing the n-acetylgalactosamine-specific lectin from helix pomatia or the glucose/mannose specific lectin from lentil beans. from the sug ... | 1985 | 3861610 |
| mutagenicity of beta-glucuronidase in the ames test. | the enzyme preparation beta-glucuronidase/arylsulphatase from helix pomatia (boehringer) caused base-pair substitutions in salmonella typhimurium ta100 and ta1535 strains within the dose range of 0.50-50 microliter per plate. no effect was observed in the ta98 strain. the presence of s9 mix did not substantially affect the mutagenic potential of beta-g. the number of induced revertants decreased continually from experiment to experiment carried out in the course of 12 weeks. | 1985 | 3892278 |
| horseshoe crab (limulus polyphemus) lectin but not garden snail (helix pomatia) lectin elicits insulin-like activities in vitro. | lectins from the horseshoe crab (limulus polyphemus) and the garden snail (helix pomatia) were tested for insulinomimetic activities in isolated rat epididymal adipocytes. the sialic acid binding horseshoe crab lectin suppressed epinephrine-induced lipolysis and augmented lipogenesis from d-[3-3h]-glucose while the n-acetylgalactosamine binding snail lectin was inactive. the results suggest that the insulin receptor on rat adipocytes contains sialic acid in its carbohydrate moiety but does not p ... | 1985 | 3893876 |
| intermediate filaments in non-neuronal cells of invertebrates: isolation and biochemical characterization of intermediate filaments from the esophageal epithelium of the mollusc helix pomatia. | to screen invertebrate tissues for the possible expression of intermediate filaments (ifs), immunofluorescence microscopy with the monoclonal antibody anti-ifa known to detect all mammalian if proteins was used (pruss, r. m., r. mirsky, m. c. raff, r. thorpe, a. j. dowding, and b. h. anderton. 1981. cell, 27:419-428). in a limited survey, the lower chordate branchiostoma as well as the invertebrates arenicola, lumbricus, ascaris, and helix pomatia revealed a positive reaction primarily on epithe ... | 1985 | 3894375 |
| lectin-gold cytochemistry reveals intercalated cell heterogeneity along rat kidney collecting ducts. | the lectin-gold technique was used to detect helix pomatia and dolichos biflorus lectin binding sites directly on semithin and thin sections of rat kidney collecting ducts. intercalated cell apical plasma membranes and the membranes of apical cytoplasmic vesicles were heavily labeled in the cortex and outer stripe of the outer medulla but were negative or very weakly labeled in the inner stripe and inner medulla. in contrast, clear cell apical membranes were labeled along the entire length of th ... | 1985 | 3919593 |
| binding of lectins to streptococcus mitis cells. studies of the specificity of ligand mediated aggregation. | previous studies have shown that the mechanism of spontaneous aggregation of streptococcus mitis atcc 903 depends on a lectin-ligand type interaction. to study the specificity of the ligand, the binding of a number of lectins of different sugar specificities to the surface of untreated, trypsin and beta-galactosidase-treated bacteria was studied by assessing aggregation. untreated bacteria were rapidly aggregated by concanavalin a (con a), wheat-germ agglutination (wga) and helix pomatia lectin ... | 1985 | 3920867 |
| patterns of urinary oestrogen excretion in female golden lion tamarins (leontopithecus rosalia). | daily urine samples were collected from 5 female golden lion tamarins (leontopithecus rosalia) over a period of 3 or more months, and urinary oestrogen concentrations were determined by radioimmunoassay. four females exhibited regular patterns of oestrogen excretion, with a peak-to-peak periodicity of 19.6 +/- 1.4 days. levels of oestrogen excretion tended to vary between, but not within, individual females. post-partum oestrogen patterns included periods of clear oestrogen cyclicity before conc ... | 1985 | 3934366 |
| polyethylene glycol-enhanced nephelometric quantification of bovine submaxillary mucin with helix pomatia lectin. | a new principle for quantification of intact mucin has been developed. the method is reproducible, simple, and sensitive at the submicrogram level and can be performed within an hour. the procedure involves incubation of mucin (bovine submaxillary mucin) and polyethylene glycol with the lectin from the snail helix pomatia and the complexes formed are quantitated by nephelometry. polyethylene glycol was found to enhance the affinitin reaction in a dose-dependent fashion. increasing concentrations ... | 1986 | 3944416 |
| duck lymphocytes. i. purification and preliminary observations on surface markers. | high yields of lymphocytes were obtained by centrifuging duck blood, collected into an equal volume of heparinized phosphate-buffered saline, over ficoll-diatrizoate, sg. 1.077, for 25 min at 200 x g. these lymphocytes did not form e rosettes under a variety of conditions, or ea rosettes with sheep erythrocytes sensitized with rabbit antibodies. high proportions (50-80%) of blood and organ (thymus, bursa of fabricius, spleen, cervical lymph node) lymphocytes had surface immunoglobulin (smig). th ... | 1986 | 3944475 |
| presence of the tn antigen on hematopoietic progenitors from patients with the tn syndrome. | the tn syndrome is an acquired clonal disorder characterized by the exposure of a normally hidden determinant, the tn antigen, on the surface of human erythrocytes, platelets, granulocytes, and lymphocytes. two distinct populations, tn positive (tn+) and tn negative (tn-), of mature hemopoietic cells are present in tn patients. to determine whether the tn antigen is already expressed on erythroid, myeloid, and pluripotent progenitors, light-density mononuclear blood cells from two patients with ... | 1985 | 3973016 |
| interaction of lectins with membrane receptors on erythrocyte surfaces. | the interactions of human genotype ao erythrocytes (red blood cells) (rbcs) with n-acetylgalactosamine-reactive lectins isolated from helix pomatia (hpa) and from dolichos biflorus (dba) were studied. binding curves obtained with the use of tritium-labeled lectins showed that the maximal numbers of lectin molecules capable of binding to human genotype ao rbcs were 3.8 x 10(5) and 2.7 x 10(5) molecules/rbc for hpa and dba, respectively. the binding of one type of lectin may influence the binding ... | 1985 | 4019586 |
| interaction energies in lectin-induced erythrocyte aggregation. | two n-acetylgalactosamine-reactive lectins, helix pomatia (hpa) and dolichos biflorus (dba), were used to study the energies involved in cell-cell interactions through the specific binding of these lectins to their membrane receptors on genotype ao human erythrocytes (red blood cells) (rbcs). the energy required to dissociate a unit of aggregated membrane area (gamma d) of two rbcs bridged by lectin molecules was determined from the shear force needed to dissociate two-cell aggregates in a flow ... | 1985 | 4019587 |
| isolation and characterization of two particle-bound oligoaminopeptidases from human meconium that are different from oligoaminopeptidase of adult small intestine. | two oligoaminopeptidases (substrate l-leucyl-beta-naphthylamide) have been separated by ion exchange chromatography after triton solubilization of meconial particles. the quantitatively major form (oligoaminopeptidase ii) has been purified to apparent homogeneity. the two meconial oligoaminopeptidases differ from each other in their polyacrylamide gel electrophoretic mobility and isoelectric points. both meconial enzymes differ from the adult enzyme by having more acidic isoelectric points and d ... | 1985 | 4067779 |
| glycoconjugate cytochemistry of the rat fundic gland using lectin/colloidal-gold conjugates and lowicryl k4m. helix pomatia lectin is a specific marker for mucous neck cells in fundic glands of the rat gastric mucosa. | the fundic gland of the rat stomach was studied using the low-temperature embedding resin lowicryl k4m and postembedding staining with lectin/colloidal-gold (cg) conjugates. intense labeling with ricinus communis agglutinin i was observed not only in mucous-producing cells but also in parietal cells. in contrast, helix pomatia agglutinin (hpa) only labeled mucous neck cells and intermediate cells between mucous neck cells and chief cells. the other epithelial cells present in the rat fundic glan ... | 1985 | 4086336 |
| [reaction of anti-a-hemagglutinin from helix pomatia with algae (chlorella pyrenoidosa) and demonstration of heterophilic agglutinins against algae in human sera]. | | 1974 | 4138180 |
| properties of an antigenic polysaccharide from corynebacterium parvum. | corynebacterium parvum strain 10390 is an antitumor agent and stimulant of the reticuloendothelial system and produces a soluble antigen towards the end of its growth cycle. this material, which is a cell wall component and can also be released from the organism by acid or alkaline hydrolysis, has been purified. it is an acidic polysaccharide of molecular weight 100,000 to 150,000 and contains galactose, glucose, fucose, n-acetylgalactosamine, n-acetylglucosamine, uronic acids, sialic acids, and ... | 1974 | 4138438 |
| [stimulation of experimentally inducdd tissue granulation by agglutinin obtained from helix pomatia l. albumin glands (anti-aph)--part ii (author's transl)]. | | 1974 | 4141666 |
| comparative studies of anti-a agglutinins from various snails of the genus helix (helix pomatia and helix aspersa). | | 1973 | 4196846 |
| cross-reactive human blood group h-active polysaccharide from helix pomatia. i. detection with catfish anti-h and eel sera. | | 1973 | 4201825 |
| [agglutination of streptococci of the group c by an agglutinin from helix pomatia]. | | 1967 | 4232426 |
| purification and characterization of a blood-group a reactive hemagglutinin from the snail helix pomatia and a study of its combining site. | | 1969 | 4241272 |
| [new polyvalent protease inhibitors for trypsin, chymotrypsin, plasmin and kallikreins from snails (helix pomatia)]. | | 1972 | 4260409 |
| [molecular weight determination on the protectine anti-hp from the albumin gland of helix pomatia (author's transl)]. | | 1974 | 4283003 |
| [investigations about an alleged lysozym-like behavior of the protectine anti-ahp from the albumen gland of the edible snail (helix pomatia) (author's transl)]. | | 1972 | 4283254 |
| the reaction of products of sequential periodate oxidation of human erythrocyte glycoproteins with hemagglutinin from helix pomatia. | | 1972 | 4351954 |
| precipitation of lipopolysaccharides from rough mutants of salmonella typhimurium by an a-hemagglutinin from helix pomatia. | | 1972 | 4557086 |
| [utilisation of the helix pomatia juice for fractionation of the cell wall of a wild strain and a mutant strain of saccharomyces cerevisiae hansen]. | | 1973 | 4585301 |
| routine identification of group-c streptococci by means of an agglutinin (protectin) from the albumen gland of the edible snail, helix pomatia. | | 1973 | 4632252 |
| [occurrence and partial purification of an unspecific cholinesterase in the albumin gland extract of helix pomatia]. | | 1972 | 4652484 |
| binding of helix pomatia a hemagglutinin to human erythrocytes and their cells. influence of multivalent interaction on affinity. | | 1973 | 4728059 |
| the human immune response to alpha-haemocyanin of helix pomatia. | | 1973 | 4749168 |
| immunological characterization of blood group a reactive protectins from helix pomatia. | | 1973 | 4760392 |
| [various comparative elements of the desamidinase systems in porcine renal tissue and in the hepatopancreatic tissue of helix pomatia lin]. | | 1973 | 4803138 |
| [agglutination of escherichia coli with an agglutinin from helix pomatia]. | | 1966 | 4862038 |
| studies on specificity and binding properties of the blood group a reactive hemagglutinin from helix pomatia. | | 1971 | 4931750 |
| a new polyvalent proteinase-inhibitor occurring in the albumin gland of helix pomatia. | | 1971 | 5173510 |
| glucose-1-phosphate-uridyl transferase of helix pomatia. | | 1967 | 5587408 |
| [comparative studies with protein gland extracts of helix pomatia and helix hortensis]. | | 1967 | 5627945 |
| a new variant of blood group a. apae. | a new variant of blood group a, termed apae, was found as an inherited characteristic in three unrelated white families. apae was distinguished from other a variants by its agglutination and absorption-elution reactions with only certain human anti-a and -a,b serums, by the red cells that reacted with anti-a from helix pomatia without the need for enzyme treatment, by enhancement of the latter reaction with bromelinization, and by the presence in the serum of an anti-a of normal avidity and tite ... | 1987 | 3603659 |
| further studies on the surface saccharides in trichomonas vaginalis strains by fluorescein-conjugated lectins. | fluorescence emitted by individual cells of several trichomonas vaginalis strains, nearly all of which were cloned, incubated with fluorescein-conjugated lectins in the absence (experimental) or presence (control) of inhibitory sugars, or else in phosphate-buffered saline alone (autofluorescence) was measured with a leitz mpv compact microfluorometer. irrespective of whether the organisms were postfixed in formalin or glutaraldehyde, the relative fluorescence emitted by the cells was closely com ... | 1985 | 3927600 |
| lectin histochemistry of scrapie amyloid plaques. | peroxidase-labeled lectins were used for detection of specific monosaccharide residues in amyloid plaques in brains of scrapie-infected mice. the lectins tested recognize the following residues: beta-d-galactosyl (ricinus communis agglutinin 120, rca-1), alpha-d-galactosyl and alpha-d-galactopyranoside (bandeirea simplicifolia aggl., bsa), alpha-d-mannosyl and alpha-d-glucosyl (concanavalin a, con a), n-acetylglucosaminyl and sialyl (wheat germ aggl., wga), sialoglycoconjugates (limulus polyphem ... | 1986 | 3962600 |
| a new surface marker on equine peripheral blood lymphocytes. i. subpopulations of lymphocytes with receptors for helix pomatia a hemagglutinin (hp). | untreated and neuraminidase-treated equine peripheral blood lymphocytes were analysed for binding of the a hemagglutinin of the snail helix pomatia (hp). for optimal staining by direct immunofluorescence, the concentration of neuraminidase had to be increased as compared to that needed for other species. moreover, higher concentrations of hp were required for optimal staining of equine lymphocytes as compared to lymphocytes from other species. even so, the maximal number of equine lymphocytes ex ... | 1985 | 3976169 |
| a new surface marker on equine peripheral blood lymphocytes. ii. characterization and separation of purified blood lymphocytes with receptors for helix pomatia a hemagglutinin (hp). | in a preceding report we have shown that the lectin helix pomatia a hemagglutinin (hp) binds to two subpopulations of neuraminidase-treated equine peripheral blood lymphocytes (pbl), constituting about 20% and 75% of pbl, respectively. the aim of the present study was to further characterize these hp+ cells in regard to other surface markers such as receptors for guinea pig erythrocytes (gpr+ cells), membrane-bound immunoglobulins (sig+ cells), receptors for activated complement (c3r+ cells) and ... | 1985 | 3976170 |
| ontogenetic analysis of some surface markers on pig lymphocytes using fluorescence-activated cell sorter. | surface markers were demonstrated on pig lymphocytes using anti-t cell-igg and anti-helix pomatia (hp) igg during prenatal and postnatal development. a fluorescence-activated cell sorter analysis of t-cell surface markers was accompanied by an image analysis to prove the association of t antigenic determinants with the plasma membrane only. we found development-dependent changes in both anti-t cell and hp surface markers in both primary and secondary lymphatic organs. the number of t-positive (t ... | 1985 | 4007711 |
| identification of a novel ganglioside on erythrocytes with blood group cad specificity. | the blood group cad antigen is a carbohydrate structure well characterized on the sialoglycoproteins of the red cell membrane from some rare individuals (blanchard, d., cartron, j. p., fournet, b., montreuil, j., van halbeck, h., and vliegenthart, j.f.g. (1983) j. biol. chem. 258, 7691-7695). however, protease treatment of whole cells did not destroy their antigenic activity which indicated that glycolipid might also be involved in the antigenic reaction. a crude ganglioside fraction was prepare ... | 1985 | 4008478 |
| reactivity of five n-acetylgalactosamine-recognizing lectins with preimplantation embryos, early postimplantation embryos, and teratocarcinoma cells of the mouse. | the expression of receptors for n-acetylgalactosamine-recognizing lectins, namely helix pomatia agglutinin (hpa), sophora japonica agglutinin (sja), bauhinia purpurea agglutinin (bpa), vicia villosa agglutinin (vva), and wistaria floribunda agglutinin (wfa) was studied in early mouse embryos and teratocarcinoma cells. each of these lectins as well as dolichos biflorus agglutinin (dba) bound differently to early embryonic cells, with the exception of vva and wfa which showed indistinguishable rea ... | 1985 | 4018458 |
| localization of binding sites for concanavalin a, ricinus communis i and helix pomatia lectin in the golgi apparatus of rat small intestinal absorptive cells. | binding sites for concanvalin a (con a), ricinus communis i agglutinin (rca i), and helix pomatia lectin (hpa) were localized in the golgi apparatus of rat small intestinal absorptive cells. a preembedment technique, a modification of the one originally used by bernhard and avrameas (exp cell res 64:232, 1971), was employed, with horse-radish peroxidase being used for cytochemical visualization. incubations were performed on 10 microns thick cryosections of duodenal segments that were fixed in a ... | 1985 | 4020101 |
| the importance of tumor markers in oral pathology. ii. cell membrane and cytoplasmic antigens as tumour markers. | important tumour markers in tumours of the oral mucosa and salivary glands are intermediate filaments of cytoskeleton, oncofetal and proliferative antigens, lectin receptors and blood group substances, enzymes, metalloproteins and viral antigens. the special occurrence of the following tumour markers was demonstrated: keratin, vimentin, carcinoembryonic antigen (cea), tissue polypeptide antigen (tpa), lectins (helix pomatia antigen = hpa, peanut agglutinin = pna), thomsen-friedenreich-antigen, b ... | 1985 | 4040631 |
| ultrastructural localization of glycocalyx domains in human kidney podocytes using the lectin-gold technique. | the lectin-gold technique was used to reveal helix pomatia lectin (hpl)-binding sites in human kidney glomeruli. hpl-binding sites were associated mainly with podocyte foot process bases, and extended over the lamina rara externa of the glomerular basement membrane. in addition, kidneys from blood group a1 donors showed labeling of capillary endothelial cells and erythrocytes. after neuraminidase digestion of the sections prior to incubation with hpl-gold complexes, hpl-binding sites appeared on ... | 1985 | 4046556 |
| [a combined radioimmunoassay method for the determination of 17 alpha-hydroxypregnenolone, 17 alpha-hydroxypregnenolone sulfate and 17 alpha-hydroxyprogesterone in human blood]. | it is well recognized that in the fetal adrenal cortex, 3 beta-hydroxysteroid dehydrogenase (3 beta-hsd) activity is lower and in fetal tissues, steroid sulfokinase (sk) is higher than in the adult. in order to clarify a part of the development processes of the adrenal cortex or steroidgenesis in humans, a combined radioimmunoassay (ria) method to estimate serum 17 alpha-hydroxypregnenolone (17-oh-delta 5 p), 17 alpha-hydroxypregnenolone sulfate (17-oh-delta 5 p-s) and 17 alpha-hydroxyprogestero ... | 1985 | 4054406 |
| lectin binding affinities of induced pancreatic lesions in the hamster model. | we examined the binding pattern of nine lectins to n-nitrosobis(2-oxopropyl)amine (bop)-induced pancreatic lesions in syrian hamsters. these lectins were arachis hypogaea (pna), dolichos biflorus (dba), griffonia simplicifolia i (gs-i), helix aspersa (haa), helix pomatia (hpa), sophora japonica (sja), ricinus communis i (rca-i), triticum vulgaris (wga) and ulex europaeus i (uea-i). all of the lectins reacted in untreated control hamsters to varying intensities with cytoplasmic components of acin ... | 1985 | 4064253 |
| a new surface marker on t lymphocytes of human peripheral blood. | neuraminidase treatment of human peripheral blood lymphocytes uncovers cell surface receptors that bind purified a hemagglutinin from the snail helix pomatia. no hemagglutinin was bound to untreated lymphocytes. binding studies with (125)i-labeled hemagglutinin suggested that the number of receptors on neuraminidase-treated lymphocytes was approximately 1.10(6)/cell. the apparent association constant for hemagglutinin binding to lymphocytes, as calculated from scatchard's plots, was 5-7.10(8) li ... | 1973 | 4583071 |
| polymorphism of esterases and tetrazolium oxidases in the roman snail, helix pomatia: a study of populations from sweden and germany. | | 1973 | 4726086 |
| modification of the composition and structure of the yeast cell wall by culture in the presence of sulfur amino acids. | growth of candida utilis and saccharomyces cerevisiae in a medium supplemented with sulfur amino acids led to synthesis and accumulation of s-adenosylmethionine, accompanied by a reduction in the cell yield, an increased sensitivity of the cell wall to snail gut enzymes (helix pomatia), as judged by spheroplast formation, and by a modification of the chemical composition of both the intact cells and their isolated walls. walls of supplemented cultures of c. utilis were three times as sensitive t ... | 1971 | 4934069 |
| purification of snail hemagglutinin from helix pomatia and its serological characteristics. | | 1971 | 5002618 |
| the composition of the cell wall of fusicoccum amygdali. | 1. the cell wall of fusicoccum amygdali consisted of polysaccharides (85%), protein (4-6%), lipid (5%) and phosphorus (0.1%). 2. the main carbohydrate constituent was d-glucose; smaller amounts of d-glucosamine, d-galactose, d-mannose, l-rhamnose, xylose and arabinose were also identified, and 16 common amino acids were detected. 3. chitin, which accounted for most of the cell-wall glucosamine, was isolated in an undegraded form by an enzymic method. chitosan was not detected, but traces of gluc ... | 1971 | 5144751 |
| bilirubin glucuronyltransferase. specific assay and kinetic studies. | 1. bilirubin glucuronide was synthesized in vitro in a system containing a rat liver microsomal fraction, udp-glucuronic acid, mg(2+) and bilirubin. the enzymic synthesis was accomplished without the addition of a bilirubin carrier. 2. azobilirubin and azobilirubin glucuronide were separated by t.l.c. and paper chromatography and the measurement of the conjugate provided a specific assay for bilirubin udp-glucuronyltransferase (ec 2.4.1.17). 3. this diazo compound was labelled when [u-(14)c]udp- ... | 1971 | 5158913 |
| the hydrolysis of glycosyl fluorides by glycosidases. | 1. alpha-d- and beta-d-glucopyranosyl, alpha-d- and beta-d-galactopyranosyl, alpha-d-mannopyranosyl and alpha-d-xylopyranosyl fluorides were hydrolysed specifically by the respective glycosidases from several sources. 2. use of specific inhibitors with a mixture of glycosidases from helix pomatia intestinal juice showed that each glycosyl fluoride was hydrolysed only by the respective glycosidase. alpha-d-glucopyranosidase and alpha-d-xylopyranosidase activities were shown to be due to different ... | 1967 | 5584010 |
| the structure of a glycopeptide isolated from the yeast cell wall. | 1. glycopeptides containing mannose were extracted from isolated yeast cell walls by ethylenediamine and purified by treatment with pronase and fractionation on a sephadex column. 2. a glycopeptide that appeared homogeneous on electrophoresis and ultracentrifugation had a molecular weight of 76000, and contained a high-molecular-weight mannan and approx. 4% of amino acids. 3. the amino acid composition of the peptide was determined. it was rich in serine and threonine and also contained glucosam ... | 1968 | 5685868 |