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schistosoma mansoni: comparison of a caribbean and african strain and experimental crossing based on compatibility with intermediate hosts and rattus rattus.to elucidate changes relative to compatibility with intermediate and definitive hosts affecting schistosoma mansoni since it was introduced to the new world, the compatibility of s. mansoni from africa (the cameroons), from the caribbean (guadeloupe), and those resulting from experimental crosses with the gastropods biomphalaria glabrata and b. pfeifferi has been studied. results show that s. mansoni, regardless of its origin or its usual snail host, always infects b. pfeifferi more successfully ...19863082664
echinostoma paraensei: hemocytes of biomphalaria glabrata as targets of echinostome mediated interference with host snail resistance to schistosoma mansoni.earlier in vivo work by lie et al. (1977) indicated that the innate resistance of the 10r2 strain of biomphalaria glabrata to pr1 schistosoma mansoni could be interfered with if the snails were infected previously with another trematode, echinostoma paraensei. we have studied this interference phenomenon using in vitro methods in an attempt to understand its mechanistic basis. hemolymph, derived from 10r2 snails infected with e. paraensei for 14-28 days, killed 25% of s. mansoni sporocysts in vi ...19863087765
serological studies of the antigens prepared from biomphalaria glabrata snails infected and uninfected with schistosoma mansoni. i. indirect immunofluorescent antibody test using infected snail and adult worm antigens. 19873106521
[current risks in the introduction of schistosomiasis into french guiana].at the moment, it seems unlikely that there is a possibility for schistosomiasis implantation in french guiana. the reasons to this are both the number of the immigrants infested by schistosoma mansoni which is quite low and the disappearance of the intermediate host biomphalaria glabrata. the disappearance of the species may be a temporary one.19873111733
serological studies of the antigens prepared from biomphalaria glabrata snails infected and uninfected with schistosoma mansoni. ii. double diffusion technique using cercarial, adult worm, infected and uninfected snail antigens. 19873121764
schistosomiasis mansoni in the dominican republic; prevalence and intensity in the city of higuey by coprological and serological methods.the prevalence and intensity of infection with schistosoma mansoni were determined in a sample of the population of the city of higuey, dominican republic. the quantitative formol-ether stool sedimentation technique of ritchie, as modified by knight et al., was performed on stools from 1,025 (65.5%) of 1,563 individuals greater than 1 year old. the highest prevalence rates (21.4%) were observed in women 25-29 years old and in men 15-19 years old (20%). the maximum fecal egg count was in the 15- ...19873124312
[susceptibility of biomphalaria glabrata, b. straminea and b. tenagophila to various strains of schistosoma mansoni]. 19873130655
schistosoma mansoni: characterization of clones maintained by the microsurgical transplantation of sporocysts.five male and 5 female clones of schistosoma mansoni were established and maintained for 3 yr by the serial microsurgical transplantation of sporocysts from infected to uninfected biomphalaria glabrata snails. the clones were initially derived from 10 randomly selected snails with monomiracidial infections. clones were characterized by several criteria, including their infectivities for mice and snails, their cercarial outputs, and their ability to produce immunity in mice. the mean infectivitie ...19883142991
drug resistance in schistosomiasis: a review.drug resistance associated with the treatment of human schistosomiasis appears to be an emerging problem requiring more attention from the scientific community than the subject currently receives. drug-resistant strains of schistosoma mansoni have been isolated by various investigators as a result of laboratory experimentation or from a combination of field and laboratory studies. review of this data appears to indicate that the lack of susceptibility observed for some of the isolated strains ca ...19873151088
why it is difficult to control biomphalaria glabrata, the vector snail of schistosomiasis.the author studied some protective behaviors of biomphalaria glabrata (vertical movements, response to molluscicides and response to water currents) and demonstrated how these activities hamper the control of this snail, which is the main vector of schistosomiasis in brazil.19873151094
[isolation of a human strain of schistosoma mansoni, sambon,1907].in this paper the authors briefly describe a human schistosoma mansoni strain from pará state, brazil. the cirene's strain was capable of infecting 71.4% of the snail vector biomphalaria glabrata (telegrafo's strain) provided by the "evando chagas" institute, belém. the cycle was completed by the infection of six mice. the thoracic and abdominal organs were examined microscopically which demonstrated the passage of the worm into the liver and lungs. the authors discuss the importance of these re ...19873151112
[resistance of biomphalaria peregrina of santa rita do sapucaí, minas gerais, brazil to infection with 3 strains of schistosoma mansoni].the descendants of the planorbid snail biomphalaria peregrina, collected in the region of santa rita do sapucaí, minas gerais, brazil, were exposed to miracidia of three strains of schistosoma mansoni: "le" strain from belo horizonte, state of minas gerais; "sj", strain from são josé dos campos, state of são paulo and "al" strain from state of alagoas. of 300 snails exposed to miracidia of the three strains, none was infected. on the other hand, 300 biomphalaria glabrata of the control groups sh ...19883152277
the production of toxic oxygen metabolites by hemocytes of different snail species.the phagocytic hemocytes of four snail species were investigated for their ability to generate reactive oxygen metabolites upon stimulation by foreign material. hemocytes of the pond snail lymnaea stagnalis and of the garden snail helix aspersa showed a luminol-dependent chemiluminescence (cl) when they phagocytosed zymosan particles. this cl was inhibited by superoxide dismutase (sod) and sodium azide, indicating the involvement of oxygen intermediates. hemocytes of the planorbid snails planorb ...19883169350
strain variation in the infectivity of schistosoma mansoni for biomphalaria glabrata. 19883201064
the host-parasite interface in molluscan schistosomiasis: biotin as a probe for sporocyst and hemocyte surface peptides.hemocytes of biomphalaria glabrata mediate the internal defensive response which, in resistant snail strains, kills sporocysts of schistosoma mansoni. lacking a gut, the sporocyst has only its tegument to interact with the host milieu (hemolymph). we have, therefore, focused our study on the surface-exposed proteins of hemocytes and sporocyst tegument. using gentle biotinylation of living systems, labelled proteins were studied after sds-page electrophoresis and transfer to nitrocellulose. resul ...19883201703
linkage relationships of seven enzyme and two pigmentation loci in the snail biomphalaria glabrata.crossing experiments with inbred stocks of the snail (biomphalaria glabrata) demonstrated that variants at two loci determining pigmentation and seven enzyme-determining loci exhibited normal mendelian segregation ratios in f2 progeny. among 39 pairwise comparisons for joint segregation, there was evidence of genetic linkage between a locus controlling mantle pigmentation (s) and 6-phosphogluconate dehydrogenase (pgd) and confirmation of a previously described linkage between esterase-2 (est-2) ...19883209852
comparison of haemolymph proteins from schistosoma mansoni (trematoda)-susceptible and resistant biomphalaria glabrata (gastropoda).1. whole and haemoglobin (hb)-depleted serum fractions from schistosoma mansoni-resistant (10-r2) and schistosome-susceptible (pr albino, m-line) strains of biomphalaria glabrata were studied using sodium dodecyl sulphate-polyacrylamide gel electrophoresis, immunoblotting and protein assays. 2. whole serum of the pr albino strain had more total protein (11.4 +/- 1.9 mg protein/ml) than the 10-r2 strain (7.8 +/- 3.0 mg protein/ml). 3. there are specific differences in the polypeptide profiles of ...19883224503
influence of experimental illumination and seasonal variation on crossbreeding mating in the snail biomphalaria glabrata.the crossbreeding activities of the schistosoma mansoni vector snail biomphalaria glabrata were counted in a laboratory aquarium throughout the year under two regimes of 12h light: 12h dark from 7 a. m. to 10 p. m. mating increased significantly in autumn and winter and just missed a significant inverse correlation with temperature and a direct one with locomotion. other similar experiments were carried out to compare mating under various illumination conditions in complete daily cycle measureme ...19883249562
schistosoma mansoni: relationship between cercarial production levels and snail host susceptibility.two populations of biomphalaria glabrata snails differing slightly in their susceptibility to schistosoma mansoni infection showed dramatic differences in cercarial output per snail. exposed to five or more miracidia, snails from a group with a 90-100% susceptibility rate (group a) produced nearly twice the number of cercariae as those from a group with a 70-80% susceptibility rate (group b). exposure of individual snails to known numbers of miracidia resulted in higher numbers of primary (mothe ...19883366217
systemic infection of laboratory-reared biomphalaria glabrata (mollusca: gastropoda) with an acid-fast bacillus. 19883373006
secretory protein biosynthesis in snail hemocytes: in vitro modulation by larval schistosome excretory-secretory products.circulating hemocytes of the snail, biomphalaria glabrata, synthesize and secrete a variety of polypeptides when maintained in vitro in serum-free medium containing [35s] methionine. sds-page/fluorographic analysis of supernatants from resistant snail (10-r2-ok strain) hemocyte cultures revealed the presence of numerous labeled polypeptides ranging in mr from 220 to 14 kda. most of these same proteins were also produced by hemocytes of a susceptible b. glabrata strain (m-line), but the overall r ...19883397815
experimental maintenance of the schistosome vector biomphalaria glabrata on an improved alginate gel.an improved alginate formulation containing spirulina algae or freeze-dried lettuce is described for experimental maintenance of biomphalaria glabrata. snail growth, food consumption and assimilation were higher on the gel containing spirulina. the increased growth was not entirely the result of the higher food consumption observed with spirulina, but appeared to be due to nutritional differences between the two nutrient sources. snails responded to decreased dietary spirulina concentration by i ...19883401070
the effect of size of m line biomphalaria glabrata on the course of development of echinostoma paraensei.m line biomphalaria glabrata snails of 4-, 6-, 8-, 10-, 12-, or 20-mm shell diameter were individually exposed to 10 miracidia each of echinostoma paraensei. snails 10 mm in size or larger were found to be significantly less likely to harbor intraventricular sporocysts than snails in smaller size categories. the percentage of snails with intraventricular sporocysts that also developed hemocyte encapsulation responses generally increased with snail size, whereas the number of snails that ultimate ...19873437347
the genetic variation of compatibility in biomphalaria glabrata and schistosoma mansoni.interactions between different biomphalaria glabrata stocks and schistosoma mansoni strains were studied. a series of inbred stocks of b. glabrata were characterized as to genetic variations in susceptibility at different ages to a series of different s. mansoni strains. a series of inbred strains of s. mansoni were characterized as to genetic variations in infectivity for b. glabrata stocks at different ages. also described is a process of selection for substrains from a single s. mansoni isola ...19873437352
[distribution of biomphalaria glabrata (say, 1818) (pulmonata, planorbidae) in the state of são paulo, brazil]. 19873452924
the effect of seasonal temperature and experimental illumination on reproductive rate in the snail biomphalaria glabrata.1. reproduction in the snail biomphalaria glabrata, a vector of schistosoma mansoni, was measured by egg production and other parameters such as number of clutches, eggs per clutch and egg hatching, under laboratory conditions. 2. reproductive rates were highest in terms of total number of eggs, clutches and hatching under constant illumination, followed by conditions similar to the natural rhythm of illumination. the greatest reduction in reproduction occurred in constant darkness. in summary, ...19873455251
behavior of biomphalaria glabrata, the intermediate host snail of schistosoma mansoni, at different depths in water in laboratory conditions.using three columns of different depths (1.10m, 8.40m and 10.40m), we investigated the possibility of biomphalaria glabrata moving towards deep regions. in the 1.10m column, we noted that locomotion can occur in two manners: 1) when the foot is in contact with the substrate: a) sliding descent; b) sliding ascent; c) creeping descent; d) creeping ascent, 2) when the foot is not in contact with the substrate: a) sudden descent without emission of air bules; b) sudden descent with emission of air b ...19873506633
[the influence of anhydrobiosis on the infestation of biomphalaria glabrata by schistosoma mansoni miracidium].410 biomphalaria glabrata (caribbean strain of guadeloupe) have been infected with one miracidium of schistosoma mansoni, 110 snails, used as controls have been kept into water; the survival rate was 96.4% after 4 weeks and 25.4% produced cercariae. 300 snails were kept on wet soil, and submitted for 6 weeks to progressive desiccation. the survival rate was 23.4% but only 9 of them produced cercariae. periodic variations of the production of male and female larvae have been shown by the weekly t ...19873507918
competitive displacement of biomphalaria glabrata by b. straminea.data on the interaction between populations of closely related species of biomphalaria, b. glabrata and b. straminea, are presented in the current paper. laboratory and field observations and experiments have shown that b. straminea has competitive advantages over b. glabrata.19873509179
analysis of the interacting components between larval schistosoma mansoni and schistosome-susceptible and resistant biomphalaria glabrata.cell-free hemolymph (serum) and hemocytes from schistosoma mansoni-susceptible (pr albino m-line) and resistant (10-r2) strains of biomphalaria glabrata were compared by sds-page, immunoblotting and radioiodination. whole serum of both snail strains is dominated by hemoglobin (hb) (mw = 160 kd). sds-page. of hb-depleted serum indicated that the 10-r2 strain has dominant polypeptides in the 50 to 30 kd range whereas pr albino snails have few low mw proteins. antibodies raised to whole pr albino a ...19873509183
population dynamics of aquatic snails in pampulha reservoir.an attempt was made to determine more accurately the density of molluskan populations in the pampulha reservoir, using the quadrate method, intending to detect the fluctuation of the populations density, the habitat conditions and the possible competitive interactions among biomphalaria tenagophila, melanoides tuberculata, pomacea haustrum and biomphalaria glabrata, through the analysis of populational parameters. among the most significative facts observed in the reservoir it has to be mentione ...19873509186
experimental infection of physa heterostropha, helisoma trivolvis, and biomphalaria glabrata (gastropoda) with echinostoma revolutum (trematoda) cercariae.gross and histologic studies were done on laboratory-raised physa heterostropha, helisoma trivolvis, and biomphalaria glabrata snails exposed individually to 100 cercariae of echinostoma revolutum. cercariae showed a predisposition for the kidneys of snails. they entered the nephridiopore, migrated up the tubular kidney, and encysted in the saccular kidney within 2 hr. considerably more cysts were in the kidney of b. glabrata at 24 hr than in h. trivolvis or p. heterostropha kidneys. encysted me ...19873572665
characterization of the 31p nmr spectrum of the schistosome vector biomphalaria glabrata and of the changes following infection by schistosoma mansoni.the 31p nuclear magnetic resonance (nmr) spectrum of the digestive gland-gonad complex (dgg) of the schistosome vector biomphalaria glabrata was characterized and the effects of infection by schistosoma mansoni noted. the in vivo spectrum was comprised of 11 peaks, 5 downfield and 6 upfield of an external 85% phosphoric acid standard. based on a variety of analytical procedures, the upfield peaks from the standard were demonstrated to be composed of carbamoyl phosphate + a mixture of 3 phosphati ...19873572667
alterations in biomphalaria glabrata plasma induced by infection with the digenetic trematode echinostoma paraensei.alterations in the noncellular hemolymph components of m line biomphalaria glabrata snails infected with the trematode echinostoma paraensei for 1, 2, 4, 8, 15, 30, or 60 days were monitored by direct microscopical examination, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) in conjunction with quantitative densitometry. a prominent particulate substance was first noted in the hemolymph of infected snails at 1 day postexposure (pe), persisted through day 15, and subsi ...19873598800
latex of "coroa de cristo" (euphorbia splendens): an effective molluscicide.an aqueous solution of the latex of "coroa de cristo" (euphorbia splendens var. hislopii) showed molluscicide action (ld90) at a concentration lower than 0.5 ppm on biomphalaria glabrata and b. tenagophila reared in laboratory and at a concentration lower than 4.0 ppm for field b. tenagophila.19863613981
effect of schistosoma mansoni on the gross lipid composition of its vector biomphalaria glabrata.1. the total lipid level of the digestive gland-gonad complex of biomphalaria glabrata was reduced by starvation and elevated following infection with schistosoma mansoni. 2. the lipid classes of b. glabrata were phospholipid, monoglyceride, free fatty acid, triglyceride, sterol ester and wax and a single unidentified lipid. the major lipid present in control, starved and infected tissue was sterol. triglyceride was a minor component of control tissue. 3. starvation reduced the relative level of ...19873621903
biomphalaria glabrata: changes in calcium reserves following parasitism by larval schistosoma mansoni.larval schistosoma mansoni have been shown to induce morphological changes to the internal calcium reserves (in particular the calcareous inclusions in type a calcium cells and to the inner, nacreous layer of the shell) of biomphalaria glabrata within 48 h of miracidial penetration. control experiments have shown that these changes were not due to either the experimental procedures used, mechanical damage or to starvation effects. the effects were, however, analogous to experimentally induced ac ...19873696764
transient non-susceptibility to schistosoma mansoni associated with atrial amoebocytic accumulations in the snail host biomphalaria glabrata.in some biomphalaria glabrata-schistosoma mansoni combinations snails are susceptible to infection as juveniles, but have variable susceptibility as adults. these snails become non-susceptible at the onset of egg-laying and typically revert to susceptibility in old age. certain stocks of b. glabrata have the capacity to form amoebocytic accumulations in the atrium, and this ability is under genetic control. the atrial amoebocytic accumulations are transitory, typically appearing at onset of egg- ...19873696776
comparative analysis of the schistosoma mansoni and schistosoma bovis cercarial production under the influence of praziquantel.snails infected with schistosoma mansoni and s. bovis and fed with a food-praziquantel mixture stop shedding cercariae for several days. the cercarial production restarts at different levels after stopping treatment: for some biomphalaria glabrata, the restarting phase of production reaches a high level whereas for other b. glabrata and all of the p. metidjensis, the level of production remains low. histological studies revealed that at the exact moment of this treatment, there is a total destru ...19863716542
quantification of parasite development in the host-parasite system biomphalaria glabrata and schistosoma mansoni.the visceral mass of biomphalaria glabrata uninfected or infected with schistosoma mansoni was serially sectioned. the amount of hepatopancreas tissue and of parasite tissue was quantified. in pool-infected snails the volume of the whole visceral mass increased very significantly until week 6 and then decreased. due to the growing parasites the volume of the visceral mass in infected snails was at most times higher when compared with uninfected snails of the same dimensions. two weeks p.i. there ...19863716543
comparison of starvation and infection by schistosoma mansoni on tissue viability and the 31p nmr spectrum of biomphalaria glabrata. 19863716546
interaction between the biomphalaria glabrata-schistosoma mansoni host-parasite system and the non-target molluscs: influence on cercarial production.the cercarial production of schistosoma mansoni by biomphalaria glabrata (target mollusc: tm) was studied in relation to the presence of non-target molluscs (ntm). nine species of ntm were tested. whatever the type of tm-ntm association (lasting association, during prepatent period and production period, association only during the exposure of the molluscs to the miracidia), the presence of ntm involved a significant increase of s. mansoni cercarial production. this increased cercarial productio ...19863746562
electron microscopic localization of chitin using colloidal gold labelled with wheat germ agglutinin.the lectin wheat germ agglutinin (wga) has a binding site which is able to bind a sequence of three n-acetyl-glucosamine residues. therefore, it has a very strong affinity for the polymers of this sugar, especially chitin. colloidal gold can be labelled with wga and used as a specific electron-dense marker for the electron-microscopic localization of chitin. the specificity of the wga-gold binding can be checked by competitive inhibition with 5-10 mm triacetyl chitotriose. the reliability of thi ...19863754855
shedding patterns of schistosoma mansoni and ribeiroia marini cercariae from a mixed infection of biomphalaria glabrata.when the snail biomphalaria glabrata is doubly infected by two species of trematode, schistosoma mansoni and ribeiroia marini, each species keeps its own cercarial shedding pattern. emergence of s. mansoni cercariae occurs during the photophase while the r. marini cercariae are shed during the night. these results demonstrate the absence of interference between the mechanisms responsible for the cercarial shedding of each species. the only difference noted concerns a two-hour shift in the peak e ...19863794287
increased oviposition and growth in immature biomphalaria glabrata after exposure to schistosoma mansoni.biomphalaria glabrata snails are known to be castrated by infection with the trematode parasite schistosoma mansoni 4-6 weeks post-infection. the pattern of oviposition in the first 35 days post-exposure (p.e.) was investigated, in snails aged 14 weeks and measuring 7-10 mm diameter which had not commenced egg-laying, by counting the numbers of eggs laid in 7-day intervals. a group of exposed snails was compared with a control non-exposed group. the exposed group included both parasitized and no ...19863797059
stimulation of schistosoma mansoni miracidia by a 80 kda glycoprotein from biomphalaria glabrata.soluble extracts of biomphalaria glabrata stimulate in vitro incorporation of methionine in schistosoma mansoni miracidia. evidence is presented that a unique 80 kda glycoprotein representing less than 0.01% of total snail proteins and uniformly distributed in the snail body, is responsible for the observed stimulation. this protein specifically acts on the miracidia and this observation suggests that this glycoprotein influences snail penetration and development of miracidia. however, the prese ...19863807942
[the discovery of biomphalaria glabrata (say, 1818) in 2 municipalities of the state of são paulo, brazil]. 19863809981
effects of environmental calcium on fecundity and cercarial production of biomphalaria glabrata (say) infected with schistosoma mansoni sambon.biomphalaria glabrata were reared in stock culture and subjected to either 7-day or 60-day acclimation periods in complex caco3 media with calcium values ranging from 1.5 mg/l to 75 mg/l. following 60-day acclimation, snails from series i were each exposed to 8 miracidia of schistosoma mansoni. snails of series ii were each exposed to a single miracidium. snails of both experimental regimens were observed for mortality, growth, rate of infection, and number of cercariae shed. series i snails wer ...19863819965
genetics of biomphalaria glabrata: linkage analysis of genes for pigmentation, enzymes, and resistance to schistosoma mansoni.the snail, biomphalaria glabrata, is a major intermediate host of the human blood fluke, schistosoma mansoni, in the americas. the inheritance and linkage relationships of a gene enabling adult snails to resist infection by a puerto rican strain of the parasite were analyzed using two laboratory stocks that differed in susceptibility, pigmentation, and five electrophoretically detectable enzyme markers. segregation ratios in second-generation intraspecific hybrids between susceptible (m-stock) a ...19853866575
effect of high dosages of bacterial challenge on acid phosphatase release from biomphalaria glabrata hemocytes. 19853920329
release pattern of aminopeptidase from biomphalaria glabrata hemocytes subjected to high-level bacterial challenge. 19853923126
assessment of schistosomiasis in the dominican republic.active transmission of intestinal schistosomiasis is currently limited to the southeastern part of the dominican republic. a population-based stool survey in 1980 detected 4 asymptomatic individuals among 114 selected at random in 2 towns and a rural community in el seibo province. the distribution of the transmitting snail, biomphalaria glabrata, considerably exceeds that of schistosoma mansoni, extending to the national district and capital city of santo domingo and well into certain central v ...19853931167
isolation and partial characterization of shared antigens of biomphalaria glabrata and schistosoma mansoni and their evaluation by the elisa and the eitb.serum from humans infected with schistosoma mansoni, when reacted with a biomphalaria glabrata soluble hepatopancreas antigen extract (bgsha) yields 2 lines of precipitation by gel diffusion and 1 by immunoelectrophoresis. the igg from the serum of a human infected with s. mansoni was coupled to cnbr-activated sepharose 4b. bgsha (8.0 mg) was then filtered through the gel and the bound antigens, denoted bgsm, eluted with hcl-glycine, ph 2.6. these bound antigens comprised 2.8% of the total bgsha ...19853932633
molluscicide activity of the "avelós" plant (euphorbia tirucalli, l.) on biomphalaria glabrata, the mollusc vector of schistosomiasis.an aqueous solution of the latex of euphorbia tirucalli collected at sites receiving large amounts of sunlight showed molluscicide action on biomphalaria glabrata, with ld50 obtained at the concentration of 28,0 ppm and ld90 at the concentration of 85,0 ppm. the toxicity of the product for fish was similar to that of bayluscide and of copper sulfate used for comparison. however, the wide distribution of the plant, its easy propagation and the simple procedure for extraction of the active substan ...19853939311
lectin-dependent cell-mediated cytotoxicity in an invertebrate model: con a does not act as a bridge.the plant lectin concanavalin a (con a) has been used in an invertebrate model of lectin-dependent cell-mediated cytotoxicity (ldcc). macrophage-like cells from the susceptible host snail biomphalaria glabrata become cytotoxic effectors when they encounter sporocysts of the parasitic trematode schistosoma mansoni that have been treated with con a. the sugar alpha-methyl mannoside and rabbit anti-con a antibodies fail to block this ldcc. con a is effective only when the target, not the effector c ...19863949370
pathophysiologic alterations in biomphalaria glabrata infected with angiostrongylus costaricensis. 19853981029
biomphalaria glabrata (gastropoda): effect of urethane on the morphology and function of hemocytes, and on susceptibility to schistosoma mansoni (trematoda). 19853998486
[factors responsible for preventing the carrying out of the schistosoma mansoni cycle in the waters of grande terre of guadeloupe (french antilles)].different surveys which have been conducted in ponds and pools in the grande terre of guadalupe, have demonstrated the absence of schistosoma mansoni transmission in spite of apparent favourable ecological conditions. four hypothesises can explain this absence of transmission: 1 - the presence of biomphalaria glabrata populations non-susceptible to the infestation by s. mansoni. 2 - ecological conditions unfavourable to the survival of parasitized snails. 3 - ecological conditions unfavourable t ...19854004031
schistosoma mansoni: lectin-dependent cytotoxicity of hemocytes from susceptible host snails, biomphalaria glabrata.normally benign hemocytes from a strain (m-line) of the snail, biomphalaria glabrata, susceptible to schistosoma mansoni, became cytotoxic toward the sporocyst stage if the parasite was first treated with the lectin, concanavalin a. concanavalin a binding was inhibitable with alpha-methyl mannoside and killing was dose-dependent. maximal levels of concanavalin a-induced cytotoxicity were comparable with levels observed when hemocytes from a resistant snail strain (13-16-r1) encountered untreated ...19854040473
plasma components which mediate cellular defences in the gastropod mollusc biomphalaria glabrata.blood plasmas from certain strains of biomphalaria glabrata are known to have components which facilitate a hemocyte-effected cytotoxic response against encapsulated schistosoma mansoni sporocysts. the possible identity of the factor(s) has been investigated. sporocysts placed in snail plasma rapidly acquire a wide variety of host plasma antigens, at least some of which are displayed on the parasite surface. plasmas from strains of snail resistant to the parasite agglutinate fixed sporocysts, wh ...19854043487
31p nmr studies on adenylates and other phosphorus metabolites in the schistosome vector biomphalaria glabrata.31p nuclear magnetic resonance spectroscopy was applied successfully to whole intact and de-shelled snails and to the isolated digestive gland-gonad tissue complex as well as other tissues of biomphalaria glabrata. several phosphorus metabolites, including atp and adp, were observed. the mean atp/adp ratio calculated for the tissue complex was 3.1 and the atp concentration was 0.73 nmoles/mg tissue fresh weight. assignments for amp, sugar phosphates, and inorganic phosphate peaks were tentativel ...19854057009
biomphalaria glabrata in haiti.biomphalaria glabrata, an intermediate host for schistosoma mansoni, was first reported in haiti in 1891 at one location in the départment du nord and in 1977 it was reported at several sites in one additional watershed. our study identifies two additional locations each on a different watershed plus a third possible site. a wide but discontinuous distribution of the snail on the north coast of haiti is confirmed (no autochthonous infections with s. mansoni have been reported). while there are m ...19854082255
orientation to light of juvenile and adult forms of melanic and albino populations of biomphalaria glabrata(say, 1818). 19854088040
[resistance of biomphalaria schrammi of arcos, minas gerais, brazil, to infection with 2 strains of schistosoma mansoni].the descendants of planorbid snail biomphalaria schrammi crosse, 1864, collected in the region of arcos, state of minas gerais, brazil, were exposed to miracidia of two strains of schistosoma mansoni: the "le" strain from belo horizonte, minas gerais state and the "sj" strain from são josé dos campos, state of são paulo. of the 172 snails exposed to miracidia of both strains, none were infected. on the other hand, 100 biomphalaria glabrata snails of the control group showed infection rates of 88 ...19854088045
[a stock of infected snails for the mass breeding of cercariae and schistosomules].a routine to maintain infected snails biomphalaria glabrata able to produce 1-2 million cercariae per week and a mean shedding of about 3,500 cercariae per snail was quantitatively described. to a monthly production of about 5.3 and 6.5 million cercariae during the years of 1982 and 1983 it was necessary to expose an average of 1,557 and 1,957 snails, respectively. the efficiency described was maintained by infecting 2,000 snails per month (infectivity index of about 60%). a maximal production o ...19854088046
[demonstration of cytoadherence properties of cells from the hemolymph of biomphalaria glabrata parasitized by schistosoma mansoni]. 19734122547
histochemical and electron microscope observations on the reproductive tract of biomphalaria glabrata (australorbis glabratus), intermediate host of schistosoma mansoni. 19694191152
increased alkaline phosphatase in the tissues and hemolymph of the snail biomphalaria glabrata infected with schistosoma mansoni. 19734196899
the effects of desiccation and starvation upon the weight, histology and ultrastructure of the reproductive tract of biomphalaria glabrata, intermediate host of schistosoma mansoni. 19734346561
laboratory observations on the biological control of biomphalaria glabrata by a species of pomacea (ampullariidae).laboratory experiments have shown that medium-sized (30-50 mm in shell height) pomacea sp. snails consumed the egg masses of biomphalaria glabrata deposited either on glass or on the leaves of salvinia, and that they also caused a high mortality rate among the newly hatched b. glabrata. when the two species of snail were kept in the same tank, the pomacea sp. caused a reduction in the b. glabrata egg density in proportion to their relative numbers and the presence of pomacea sp. prevented coloni ...19724537485
molluscicidal activity of trifenmorph in field trials.trifenmorph granules provided 100% control of biomphalaria glabrata and b. tenagophila when applied at 2 kg of active ingredient per hectare, except at one site with a ph of 5.6 where the snail population was reduced by only 50%. granulation was found to facilitate application by either hand or mistblower, and reduces the risk of infection to the spray team. applied to canal margins the granules had a residual toxic effect.19734546869
interference by unsusceptible aquatic animals with the capacity of the miracidia of schistosoma mansoni sambon to infect biomphalaria glabrata (say) under field-simulated conditions in st. lucia, west indies. 19724628268
the influence of temperature on the biology of biomphalaria glabrata (say), intermediate host of schistosoma mansoni on st. lucia, west indies. 19724634780
exposure of caged biomphalaria glabrata (say) to investigate dispersion of miracidia of schistosoma mansoni sambon in outdoor habitats in st. lucia. 19724641399
effects of some physico-chemical factors on the infection of biomphalaria glabrata (say) by miracidia of schistosoma mansoni sambon in st. lucia, west indies. 19724641400
effect of water depth on the infection of biomphalaria glabrata by miracidia of st. lucian schistosoma mansoni under laboratory and field conditions. 19724641401
an investigation of the interactions of some factors influencing the infectivity of schistosoma mansoni miracidia to biomphalaria glabrata. 19734687432
effect of marisa cornuarietis on populations of biomphalaria glabrata in farm ponds of puerto rico. 19734688423
ribeiroia marini: pathogenesis and larval trematode antagonism in the snail, biomphalaria glabrata. 19734706118
field studies on the population dynamics of biomphalaria glabrata, intermediate host of schistosoma mansoni on the west indian island of st. lucia. 19734706569
field studies on the transmission of schistosoma mansoni and on the bionomics of its intermediate host, biomphalaria glabrata, on st. lucia, west indies. 19734706570
field investigations on the effect of other aquatic animals on the infection of biomphalaria glabrata by schistosoma mansoni miracidia. 19734711664
location of biomphalaria glabrata (say) by miracidia of schistosoma mansoni sambon in natural standing and running waters on the west indian island of st. lucia. 19734732026
the effect of photoperiod inversion upon schistosoma mansoni cercarial emergence from biomphalaria glabrata. 19734741637
susceptibility of adult biomphalaria glabrata to schistosoma mansoni infection. 19734745234
primary cultures of embryonic cells from the snail biomphalaria glabrata. 19734745241
rhythmic emergence of schistosoma mansoni cercariae from biomphalaria glabrata: influence of the temperature. 19734755616
alterations in total serum proteins and protein fractions in biomphalaria glabrata parasitized by schistosoma mansoni. 19734761358
the effect of water temperature on the penetration and development of st. lucian schistosoma mansoni miracidia in local biomphalaria glabrata. 19734768714
[study of the susceptibility of biomphalaria glabrata from ourinhos (são paulo), to schistosoma mansoni from belo horizonte (minas gerais) and são josé dos campos (são paulo)]. 19734789392
the effect of parasitism and starvation on carbohydrate reserves of biomphalaria glabrata. 19744819580
dynamics of populations of biomphalaria glabrata and the von foerster equation. 19744831921
14c uptake by schistosoma mansoni from biomphalaria glabrata exposed to 14c-glucose. 19744833175
echinostoma lindoense: larval antigens from the snail intermediate host, biomphalaria glabrata. 19715003359
schistosoma mansoni: alterations in total protein and hemoglobin in the hemolymph of infected biomphalaria glabrata. 19725016592
the effect of sublethal concentrations of the molluscicide triphenyl lead acetate on biomphalaria glabrata (say) and on the development of schistosoma mansoni in the snail. 19725038420
on the reported occurrence of an ecdysone-like steroid in the freshwater snail, biomphalaria glabrata (pulmonata; basommatophora), intermediate host of schistosoma mansoni. 19725039344
incorporation of 59 fe in the snail biomphalaria glabrata parasitized by schistosoma mansoni. 19725042055
rhythmic emergence of schistosoma mansoni cercariae from biomphalaria glabrata: control by illumination. 19725046888
genetic factors in the susceptibility of juvenile biomphalaria glabrata to schistosoma mansoni infection. 19725050093
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