| growth of the y strain of trypanosoma cruzi in an hx25-modified defined medium. | | 1977 | 325189 |
| trypanosoma cruzi: modification of macrophage function during infection. | infection of mice with trypanosoma cruzi and subsequent intraperitoneal challenge with heat-killed trypanosomes elicits peritoneal macrophages which display in vitro microbicidal activity against trypomastigotes of t. cruzi. these cells also display other activated properties including rapid spreading, intense membrane activity, secretion of high levels of plasminogen activator, and ingestion mediated by the c3 receptor. an intravenous infection with bcg, followed by an intraperitoneal challenge ... | 1977 | 327012 |
| trypanosoma cruzi: the immunological induction of macrophage plasminogen activator requires thymus-derived lymphocytes. | in this article we describe methods in which unstimulated mouse peritoneal macrophages were induced to secrete high livels of plasminogen activator under in vitro conditions. the exposure of sensitized peritoneal or spleen cell populations from trypanosoma cruzi-infected animals to either viable or heat-killed trypanosomes lead to the release of an inducing factor(s). maximal levels of plasminogen activator secretion are achieved by the incubation of such factors (s) with unstimulated macrophage ... | 1977 | 327013 |
| catabolism of deoxythymidylate in some trypanosomatids. | an initial observation concerning the failure of [3h]thymidine at high specific activity to be incorporated into the dna of crithidia fasciculata for more than a brief initial period has been correlated with the presence at high specific activity in the organism of a thymidine phosphorylase activity with an equilibrium in the direction of catabolism. this enzyme degrades thymidine to thymine which is not utilized by the organism. the enzyme has also been shown to be present in a number of other ... | 1977 | 327402 |
| geographical extension in a new ecological association of panstrongylus humeralis (hemiptera: reduviidae) natural host of trypanosoma cruzi in panama. | | 1977 | 328884 |
| comparative drug sensitivities of culture forms of trypanosoma cruzi and trypanosoma dionisii. | | 1977 | 329144 |
| eosinophils and not lymphoid k cells kill trypanosoma cruzi epimastigotes. | | 1977 | 329145 |
| some observations on the effect of ro 7-1051 on trypanosoma cruzi, particularly in cell culture. | | 1977 | 330210 |
| trypanosoma cruzi. surface change characteristics of cultured epimastigotes, trypomastigotes and amastigotes. | | 1977 | 331436 |
| [effect of parasite density of ooencyrtus trinidadensis (chalcidoidea, encyrtidae), an endophagus parasite of eggs of rhodnius prolixus, vector of chagas' disease in venezuela]. | | 1977 | 331437 |
| effects of 9-hydroxy ellipticine on in vitro transcription of trypanosoma cruzi dnas. | | 1977 | 332175 |
| fine structure of the trypomastigote form of trypanosoma cruzi isolated from acellular culture by passage in column. | | 1977 | 333521 |
| the citrate synthase from trypanosoma cruzi. | | 1977 | 335036 |
| comparative stability of epimastigotes and trypomastigotes in different wash solutions. | | 1977 | 335037 |
| surface charge of trypanosoma cruzi. binding of cationized ferritin and measurement of cellular electrophoretic mobility. | the surface charge of epimastigote and trypomastigote forms of trypanosoma cruzi was evaluated by means of binding of cationized ferritin to the cell surface as visualized by electron microscopy, and by direct measurements of the cellular microelectrophoretic mobility (epm). epimastigote forms had a mean epm of -0.52 micrometer-s-1-v-1-cm and were lightly labeled with cationized ferritin. in contrast, bloodstream trypomastigotes had a much higher epm (-1.14), and the surface was heavily labeled ... | 1977 | 335054 |
| [effect of beta and alpha-lapachone on the production of h202 and on the growth of trypanosoma cruzi]. | addition of beta-lapachone to the epimastigote (culture) form of trypanosoma cruzi, suspended in saline, buffered-isotonic medium (ph 7.2), determined the appearance of large amounts of h2o2 in the suspension medium, as measured spectrophotometrically by formation of the h2o2 horse radish peroxidase complex. under similar conditions, alpha-lapachone did not induce h2o2 formmation. using nadh as electron donor, beta-lapachone (not alpha-lapachone) increased significantly the rate of h2o2 generat ... | 1977 | 339293 |
| a comparative study of the behavior of venezuelan and brazilian strains of trypanosoma (schizotrypanum) cruzi in the venezuelan invertebrate host (rhodnius prolixus). | | 1977 | 339313 |
| the resistance to human plasma of trypanosoma brucei, t. rhodesiense and t. gambiense: iii. clones of two plasma-resistant strains. | tests for resistance to human plasma were made on six clones of a stabilate of trypanosoma rhodesiense (lump 10) which was calculated to contain about 3,000 resistant trypanosomes per million. two of the clones were not resistant and four were only subresistant. tests were also made on 12 lines (clones) of a stabilate of polymorphic trypanosomes isolated from tsetse flies. one of them, etat 10, had infected a laboratory worker and was found to be fully resistant to human plasma; the other lines ... | 1977 | 339423 |
| growth-inhibition drug test with trypanosoma cruzi culture forms. | a 48-hr drug screening test is described which evaluates inhibition of exponential growth of t. cruzi culture forms by electronic cell count. about 80% of drugs active in vivo produced a greater than 50% growth inhibition, whereas among compounds inactive in vivo, only 19.6% induced such inhibition. advantages of this test are low cost, rapid results, small amounts of drugs needed, and feasibility without animal facilities. comparative studies showed that culture forms are not suitable for scree ... | 1977 | 340677 |
| proteolytic activites in cell extracts of trypanosoma cruzi. | cell extracts of culture forms of trypanosoma cruzi are capable of hydrolysing substances belonging to 4 different groups of protease substrates: (a) substrates for trypsin-like enzymes: benzoyl-arginine-p-nitroanilide and benzoylarginine-naphtylamide; (b) substrates for aminopeptidases: leucyl, lysl and glutamyl-beta-naphtylamide; (c) a substrate fochymotrypsin-like enzymes: carbobenzoxy-l-tyrosine-p-nitorphenylester, and (d) a nonspecific substrate for a broad range of proteases: azocasein. so ... | 1977 | 340678 |
| the number of trypomastigotes of trypanosoma cruzi, required to infect rhodnius prolixus. | | 1977 | 341267 |
| trypanosoma cruzi soluble antigen. role in immunofluorescence, complement fixation and inhibition of hemagglutination tests. | | 1977 | 341268 |
| [studies on reservoirs and wild vectors of trypanosoma cruzi. lxvi: evolution cycle of trypanosoma cruzi in hemocel of panstrongylus megistus]. | | 1977 | 343195 |
| [external phenotypic differences between interspecies hybrids and their respective species, vectors of chagas' disease in brazil (triatominae, reduviidae)]. | | 1978 | 345369 |
| observation on the interaction of peritoneal macrophages with trypanosoma cruzi. ii. intracellular fate of bloodstream forms. | | 1977 | 345392 |
| lipopolysaccharide from trypanosoma cruzi. | | 1978 | 345535 |
| protein typing by disc electrophoresis of some species of trypanosomes with special emphasis to trypanosoma cruzi. | proteins extracted from culture forms of t. cruzi, t. cruzi-like strains, t. rangeli, t. conhorrini and t. dionisii were separated by disc electrophoresis. the electrophoretic protein patterns of all strains examined were highly reproducible. the results indicate that each of these strains have their own "fingerprints" and the trypanosoma species could be identified by some typical specific protein bands. however, it was not possible to distinguish between the human strains of t. cruzi and the t ... | 1978 | 347650 |
| cyanide-sensitive and insensitive respiration of trypanosoma cruzi. | | 1978 | 350173 |
| tricarboxylic acid cycle operation at the kinetoplast-mitochondrion complex of trypanosoma cruzi. | | 1978 | 350277 |
| antibody dependent cell-mediated cytotoxicity of trypanosoma cruzi: the release of tritium-labelled rna, dna and protein. | the cytotoxicity of normal rat spleen cells to antibody-coated trypanosoma cruzi epimastigotes has been studied by assaying the release of [3h]-labelled macromolecules from the parasites. the release of thymidine (dna) is slower than the release of uridine (rna), suggesting that the nucleus is broken down more slowly than the cytoplasmic membrane. less than 50% of the leucine (protein) is released when the parasites are lysed, whereas uridine (rna) is almost totally released. in practical terms ... | 1978 | 351526 |
| haemoflagellates: commercially available liquid media for rapid cultivation. | the successful cultivation of a variety of haemoflagellates in three different liquid media is reported. these media include medium 199, grace's insect tissue-culture medium and schneider's drosophila medium, each in combination with 30% (v/v) foetal calf serum. these media were used to cultivate old and new world species of visceral and cutaneous human leishmania, as well as leishmania species isolated from sandflies, rodents, and reptiles. four strains of trypanosoma cruzi, an isolate of t. r- ... | 1978 | 351527 |
| separation with cm-cellulose of the trypomastigote form of trypanosoma cruzi from the forms grown in fibroblast cell cultures. | | 1977 | 352340 |
| trypanosoma cruzi: ultrastructural, cytochemical and freeze-fracture studies of protein uptake. | | 1978 | 352715 |
| trypanosoma cruzi: isolation and characterization of a protease. | | 1978 | 352716 |
| trypanosoma cruzi: in vitro induction of macrophage microbicidal activity. | normal, resident and inflammatory mouse peritoneal macrophages can be induced to display microbicidal activity against trypomastigotes of trypanosoma cruzi by exposure to products from antigen-pulsed, sensitized spleen cell populations. optimal macrophage microbicidal activity was achieved by constant exposure and daily renewal of the spleen cell factors. macrophages obtained after an intraperitoneal injection of mild inflammatory agents were rapidly induced, displaying trypanocidal activity 24 ... | 1978 | 353218 |
| [reservoirs and wild vectors of trypanosoma cruzi. lxvii: various aspects of the behavior of different t. cruzi strains in the coelomic cavity of panstrongylus megistus]. | | 1978 | 353908 |
| blood cultures of trypanosoma cruzi using vacuum culture tubes. | | 1978 | 354116 |
| biochemical characterization of some species of trypansoma (schizotrypanum) from bats (microchiroptera). | trypanosoma (schizotryanum) species isolated from bats (microchirotera) in europe and latin america were examined by determining the buoyant densities of their nuclear and kinetoplastic deoxyribonucleic acid (dna) and the electrophoretic patterns of six isoenzymes. by these criteria they were separated into three distinct groups -- two from europe (t. dionisii and t. vespertilionis) and one from america. t. dionisii was also separable by its morphology in vitro. geographical location and dna buo ... | 1978 | 354417 |
| trypanosoma cruzi: location of a specific antigen on the surface of bloodstream trypomastigote and culture epimastigote forms. | | 1978 | 354954 |
| trypanosoma cruzi: identification of a cell surface polysaccharide. | | 1978 | 354955 |
| trypanosoma cruzi: intracellular stages grown in a cell-free medium at 37 c. | | 1978 | 354956 |
| trypanosoma cruzi: in vitro interactions between cultured amastigotes and human skin-muscle cells. | | 1978 | 354958 |
| [molecular forms of alpha-hydroxyacid dehydrogenase in trypanosoma cruzi]. | | 1978 | 355774 |
| [nucleic acid and proteins content in trypanosoma cruzi epimastigotes]. | the nucleic acids and protein content in epimastigote forms of trypanosoma cruzi during its exponential growth in vitro were studied. the parasites were cultivated in a diphasic blood-agar medium at 28 degrees c. the rna extraction and hydrolisis were performed by the method of fleck and begg and the acid hydrolisis of dna precipitate was done according to webb and lindstrom. schneider's colorimetric method was used for ribose and desoxyribose measurement, employing as reagents orcein and diphen ... | 1978 | 356127 |
| extracellular "in vitro" evolution of metacyclic trypomastigotes isolated from trypanosoma cruzi cultures. | | 1978 | 356159 |
| antitrypanosomal effect of allopurinol: conversion in vivo to aminopyrazolopyrimidine nucleotides by trypanosoma curzi. | the parasite trypanosoma cruzi metabolizes allopurinol by a sequential conversion to allopurinol mononucleotide and aminopurinol mononucleotide. the latter is incorporated into rna. this transformation of a widely used innocuous agent, allopurinol, into a toxic adenine analog appears to account for the antiprotozoan effect of allopurinol. these unique enzymatic activities appear to occur only in t. cruzi and the pathogenic lesihaminae. allopurinol may serve as a model for the synthesis of simila ... | 1978 | 356267 |
| [phospholipids and other lipid components of trypanosoma cruzi. comparative study in tulahuén, es and brazil strains]. | | 1978 | 357886 |
| [hydrogen peroxide metabolism in trypanosoma cruzi]. | | 1978 | 357887 |
| [aerobic degradation of glucose by trypanosoma cruzi]. | | 1978 | 362118 |
| [leucine aminotransferase activity in trypanosoma cruzi]. | | 1978 | 362119 |
| the cell surface of trypanosoma cruzi: cytochemistry and freeze-fracture. | the ultrastructure of epimastigotes of trypanosoma cruzi, obtained from acellular cultures, and bloodstream trypomastigotes, isolated from infected mice, were studied by thin-sectioning and freeze-fracturing techniques. epimastigotes showed a thin (5 nm) surface coat when stained with ruthenium red, while the surface coat of trypomastigotes was more prominent (15 nm thick). both p and e faces of the plasma membrane of t. cruzi had roughly the same number of intramembranous particles (imp) as see ... | 1978 | 363731 |
| immunization and enhancement properties of the circulating antigens of trypanosoma cruzi. | | 1978 | 364787 |
| plasma membrane vesicles isolated from epimastigote forms of trypanosoma cruzi. | membrane vesicles can be obtained from epimastigote forms of trypansoma cruzi by incubating cells with either cross-linking reagents or acid ph. acetate, phtalate or citrate, at ph 4.0, but not at higher ph values, were able to induce plasma membrane vesiculation. vesicles have been purified by sucrose density centrifugation and their membrane origin was demonstrated by the following criteria: (a) vesicles are 5--10 times richer in protein-bound iodine when they are prepared from cells previousl ... | 1979 | 365244 |
| purification of an adenylyl cyclase-containing plasma membrane fraction from trypanosoma cruzi. | a fraction containing plasma membrane fragments has been purified from epimastigote forms of trypanosoma cruzi. cells were broken by sonic vibration under well defined conditions and membranes were isolated by differential centrifugation and equilibrium centrifugation in sucrose gradients. the co-purification (approximately 10-fold) of adenylyl cyclase and plasma membrane-bound radioactive iodine is highly suggestive of the localization of this enzyme in the plasma membrane of t. cruzi. determin ... | 1979 | 365245 |
| trypanosoma cruzi: cultivation in macromolecule-free semisynthetic and synthetic media. | | 1978 | 365557 |
| [differentiation by microimmunofluorescence of t. cruzi and t. cruzi-like strains from t. conorhini and t. rangeli, using protection from the sponge aaptos papillata (author's transl)]. | carbohydrates containing terminal nonreducting d n-acetylgalactosamin or d n-acetylglucosamin were identified at the cell surface of trypanosoma cruzi by an indirect immunofluorescence test using the lectin of the sponge aaptos papillata. with the method described in this study t. cruzi and t. cruzi-like strains are easy to distinguish from t. rangeli, but not from t. conorhini. the immunofluorescence test presented here may be very helpful for certain diagnostic problems. | 1978 | 366941 |
| in vitro and in vivo evaluation of the toxicity of 1,4-naphthoquinone and 1,2-naphthoquinone derivatives against trypanosoma cruzi. | the toxic effect of lapachol, beta-lapachone and several 1,2-naphthoquinone derivatives on the growth, viability and infectivity of trypanosoma cruzi were compared. beta-lapachone was the most active compound in vitro. no inhibition was observed in suspensions which contained inactivated foetal calf serum or rabbit haemoglobin solution. the infectivity of trypomastigotes in mice was not affected when cells were previously incubated with beta-lapachone or one of several other naphthoquinone deriv ... | 1978 | 367298 |
| growth of trypanosoma cruzi in vitro, unaffected by sodium salicylate. | | 1978 | 367301 |
| ceramide and inositol content of the lipopeptidophosphoglycan from trypanosoma cruzi. | | 1978 | 369561 |
| [wild reservoirs and vectors of trypanosoma cruzi. lxx. behavior of t. cruzi strains isolated from wild animals, in the ricman and robson test]. | | 1978 | 370918 |
| [the kinetoplast of trypanosoma cruzi during cell differentiation (proceedings)]. | | 1978 | 373536 |
| selective inhibition of k-dna synthesis of trypanosoma cruzi and leishmania tarentolae by berenil and ethidium bromide [proceedings]. | | 1978 | 373537 |
| [phospholipid metabolism in t. cruzi (proceedings)]. | | 1978 | 373538 |
| drugs inducing generation of free radicals in trypanosoma cruzi [proceedings]. | | 1978 | 373539 |
| [changes in the t. cruzi membrane induced by immune serum. i. phospholipid metabolism (proceedings)]. | | 1978 | 373540 |
| [characterization of sub-cellular fractions of trypanosomatidae (proceedings)]. | | 1978 | 373541 |
| trypanosoma cruzi cell surface proteins: identification of one major glycoprotein. | | 1979 | 374115 |
| evidence for the plasma membrane localization of carbohydrate-containing macromolecules from epimastigote forms of trypanosoma cruzi. | | 1979 | 374124 |
| isolation of trypanosoma cruzi from the blood of infected mice by column chromatography. | trypomastogotes of three strains of trypanosoma cruzi were isolated from the blood of infected mice employing lymphoprep for separation of the red blood cells and a column of deae cellulose for removal of white cells and platelets. an average recovery of 45 to 58 percent of actively motile, infective organisms, free of contaminating blood cells was obtained. protein and carbohydrate assays of the separated organisms revealed significant differences between the tulahuen, a reticulotropic strain, ... | 1979 | 375218 |
| [effect of hormones (progesterone and testosterone) on the pathogenicity of trypanosoma cruzi]. | | 1978 | 375351 |
| [comparative study of the pathogenicity of 4 strains of t. cruzi in white mice]. | | 1978 | 375352 |
| effect of polyamines on protein kinases activities from trypanosoma cruzi. | an interaction of polyamines with protein kinases activities from t. cruzi epimastigotes is demonstrated. spermine and spermidine and less pronounced putrescine are found to inhibit protein kinases activities. in the extract of t. cruzi three protein kinases are distinguished on account of molecular weight (greater than 200 000, 95 000 and 40 000) and preference for acceptor proteins (phosvitin and histones). especially the activity of the high molecular weight protein kinase which phosphorylate ... | 1979 | 375514 |
| activation of macrophages in vivo and in vitro. correlation between hydrogen peroxide release and killing of trypanosoma cruzi. | as reported previously, mouse peritoneal macrophages could be activated to kill intracellular trypomastigotes of trypanosoma cruzi, the agent of chagas' disease, in either of two ways: by immunizing and boosting the mice (3), or by culturing resident or inflammatory macrophages in spleen cell factor(s) (scf) in vitro (2). macrophages activated in vivo became less trypanocidal with time in culture, and cells activated in vitro lost trypanocidal capacity when csf was removed (2). in the present st ... | 1979 | 376774 |
| effect of purification by deae-cellulose column on infectivity of trypanosoma cruzi blood forms. | | 1979 | 376814 |
| characterization by sedimentation analysis of kinetoplast dna from trypanosoma cruzi at different stages of culture. | the kinetoplast dna networks of trypanosoma cruzi exist under two forms which have been studied by equilibrium density centrifugation in cscl gradients containing ethidium bromide and by band sedimentation analysis. the relative proportion of the two forms has been measured and varies significantly between the exponential and stationary phase of growth, suggesting that one of these forms is a replicative intermediate. both forms exhibit very high sedimentation coefficients. the sedimentation vel ... | 1979 | 377236 |
| epoxide hydrase in trypanosoma cruzi epimastigotes. | 1. microsomal fractions from trypanosoma cruzi epimastigotes catalyze the hydration of styrene oxide to styrene glycol. the activity is linear up to 45 min of incubation, is proportional to microsomal protein concentration within certain range, and has an optimum ph of 8.5. 2. double-reciprocal plots indicate a km value of 5.3 . 10(-4) m for styrene oxide and a v of 29.6 pmol of styrene glycol formed/min per mg protein at 37 degrees c. 4-chlorophenyl-2,3-epoxypropyl either (ki = 2.08 . 10(-4) m) ... | 1979 | 378266 |
| trypanosoma cruzi: defined medium for continuous cultivation of virulent parasites. | | 1979 | 378678 |
| [trypanosoma cruzi in cultures of spinal ganglia of chick embryo, seen in intereference contrast and electron microscopy (proceedings)]. | | 1979 | 380424 |
| a re-examination of purine and pyrimidine synthesis in the three main forms of trypanosoma cruzi. | | 1979 | 383542 |
| a morphological study of the interaction between trypanosoma cruzi and rat eosinophils, neutrophils and macrophages in vitro. | the interaction between trypomastigotes of t. cruzi and purified rat eosinophils, neutrophils and macrophages has been studied. macrophages induced by dextran, became infected and the parasites multiplied as amastigotes, eventually being released as trypomastigotes into the medium. eosinophils show an antibody-dependent phagocytosis, while neutrophil phagocytosis is enhanced by antibody. electron microscopy showed that once inside the granulocyte phagosome, the trypomastigotes transform to amast ... | 1979 | 383733 |
| electron microscopic study of trypanosoma cruzi. | | 1979 | 383971 |
| acetate oxidation by bloodstream forms of trypanosoma cruzi. | bloodstream forms of trypanosoma cruzi had a substantial increase in respiration in the presence of acetate. oxidation of acetate took place via the tricarboxylic acid cycle and involved an antimycin a-sensitive respiratory pathway. oxygen uptake in the presence of acetate was a sensitive to antimycin a inhibition as was co2 production. there was a 6--7% residual o2 uptake which was not inhibited by high antimycin concentrations. human anti-t. cruzi sera had no effect on oxygen uptake. | 1979 | 385857 |
| isolation of pure metacyclic trypomastigotes of trypanosoma cruzi from triatomine bugs by use of a deae-cellulose column. | | 1979 | 390110 |
| [effect of antimycin a on the respiration and growth of trypanosoma cruzi]. | | 1979 | 390297 |
| two forms of 'malic' enzyme with different regulatory properties in trypanosoma cruzi. | 1. cell-free extracts from culture epimastigotes of trypanosoma cruzi contained two forms of nadp+-linked 'malic' enzyme (ec 1.1.1.40), i and ii, with the same molecular weight but different electrophoretic mobilities and kinetic and regulatory properties. 2. the apparent km for l-malate was lower for 'malic' enzyme i, with hyperbolic kinetics, whereas the kinetic pattern for 'malic' enzyme ii was slightly sigmoidal (h 1.4). the kinetics for nadph were hyperbolic for 'malic' enzyme i, and very c ... | 1979 | 393256 |
| antibody-induced capping of the intracellular stage of trypanosoma cruzi. | intracellular-stage trypanosoma cruzi were isolated from infected mice. the effect of specific human antibodies on the membrane surface antigens was studied in vitro, using the immunofluorescent technique. the immune serum induced the aggregation of surface antigens, forming polar caps on the amastigotes. | 1979 | 393632 |
| [immunoenzymatic diagnosis (elisa) of human african trypanosomiasis. (a comparative study of several antigens) (author's transl)]. | a comparative study, by elisa, of purified exoantigen and of five crude antigenic extracts from several strains of t.b. gambiense and t.b. brucei has shown a wide range of results. the best sensitivity is obtained with virulent strains from human origin. for more accuracy, an association of such antigens would be preferable. | 1979 | 393937 |
| [lectin receptors on the surface of trypanosoma cruzi epimastigotes]. | | 1979 | 393950 |
| purine metabolism in trypanosomatids. | purine nucleotide biosynthesis was studied in culture forms of trypanosoma cruzi strain y, crithidia deanei (a reduviid trypanosomatid with an endosymbiote) and an aposymbiotic strain of c. deanei (obtained by curing c. deanei with chloramphenicol). trypanosoma cruzi was found to synthesize purine nucleotides only fring incorporated into both adenine and guanine nucleotides. similar results were obtained with guanine, indicating that this flagellate has a system for the interconversion of purine ... | 1979 | 395295 |
| [studies on the persistence of the infectivity of trypanosoma cruzi. ii. persistence of infectivity of t. cruzi in dead bugs (author's transl)]. | investigations into the viability time of t. cruzi in dead bugs experimentally infected with "peru" strain showed a maximum of 9 days for dipetalogaster maximus and 8 for triatoma infestans maintained at ambient temperature (26 degrees c). on refrigeration survival was increased to 60 days. in routine xenodiagnosis in the refrigerator 90 day survival was noted of both t. cruzi and the vector bug. | 1979 | 396604 |
| trypanosoma cruzi: interaction with vertebrate cells. dna synthesis and growth of intracellular amastigotes and their relationship to host cell dna synthesis and growth. | dna synthesis of intracellular trypanosoma cruzi amastigotes, following the infection of bovine embryo skeletal muscle (besm) cells, was studied by autoradiography. after penetration, there was a prereplicative lag period (similar to or approximately 12 h) followed by a synchronous round of dna synthesis which was found to be independent of parasite number/besm cell cand the host cell dna synthesis cycle. parasite reproduction occurred, for the first time, at approximately 21 h postinfection. it ... | 1979 | 397342 |
| [insects and protozoa capable of inhibiting the breeding of triatomidae and the detection of trypanosoma cruzi in fecal material from triatomidae]. | | 1977 | 397544 |
| genetic modification of macrophage functions in relation to antibody responsiveness and resistance to infection. | | 1979 | 397751 |
| [various aspects of the behavior of wild strains of trypanosoma cruzi in mice and calomys callosus (rodentia)]. | | 1979 | 398584 |
| electronmicroscopic observations on lysis of trypanosoma cruzi epimastigote by normal rabbit serum. | the lytic effects of serum from a non-immunized rabbit on epimastigotes of trypanosome cruzi were studied by electronmicroscopy. the first detectable change was the appearance of a fuzzy deposit over the whole surface of the epimastigote. soon after this, pellicular microtubules disappeared without change of axonemal microtubules. circular lesions were observed by negative staining, corresponding to the lesion of antibody-mediated lysis caused by complement. | 1979 | 399585 |
| [chronic chagas myocarditis in mexico. 3d case confirmed by parasitoscopic examination]. | | 1979 | 400305 |
| glutamate dehydrogenase and aspartate aminotransferase in trypanosoma cruzi. | 1. the cultured, epimastigote-form of trypanosoma cruzi contains nadp-linked glutamate dehydrogenase (ec 1.4.1.4), with a molecular weight of about 280,000, similar to the enzyme from plasmodium chabaudi and different from the enzymes from higher animal sources. 2. t. cruzi also contains aspartate aminotransferase (ec 2.6.1.1), with properties similar to those of the enzyme from mammals. 3. the concerted action of the transaminase and glutamate dehydrogenase might be responsible for the producti ... | 1977 | 400947 |
| lipid composition of trypanosoma cruzi. | 1. lipid composition of trypanosoma cruzi epimastigote form in culture consist of 35% of phospholipids and 65% of neutral lipids. 2. among the phospholipids, phosphatidylcholine is the more abundant (44%), followed by phosphatidylethanolamine (28%), phosphatidylinositol (12%), sphingomyelin (4%), and smaller amounts of cardiolipin, phosphatidic acid, lysolecithin, phosphatidylserine (traces), and an unidentified phospholipid (3%). 3. pulse labeling with 32p showed highest specific incorporation ... | 1977 | 400952 |
| inhibition of the nadp-linked glutamate dehydrogenase from trypanosoma cruzi by sulfhydryl reagents. | 1. the nadp-linked glutamate dehydrogenase purified from epimastigotes of trypanosoma cruzi was strongly, but not completely, inhibited by sulfhydryl reagents, in the presence of tris-hcl or phosphate buffers. 2. the enzyme modified by preincubation with o-iodosobenzoate had a kinetic behaviour different from that shown by the enzyme modified with other inhibitors, such as n-ethylmaleimide or p-chloromercuribenzoate. 3. the inhibition by o-iodosobenzoate was additive with the inhibition by the o ... | 1979 | 400963 |
| immunosuppression and circulating trypanosoma cruzi in a kidney transplant recipient. | | 1977 | 402046 |