| cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase. | cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material. consolidated bioprocessing (cbp), combining cellulase production, saccharification, and fermentation into one step, has been proposed as the most efficient way to reduce the production cost of cellulosic bioethanol. in this study, we developed a cellulolytic yeast consortium for cbp, based on the surface display of c ... | 2013 | 23383678 |
| construction of a novel selection system for endoglucanases exhibiting carbohydrate-binding modules optimized for biomass using yeast cell-surface engineering. | to permit direct cellulose degradation and ethanol fermentation, saccharomyces cerevisiae by4741 (δsed1) codisplaying 3 cellulases (trichoderma reesei endoglucanase ii [eg], t. reesei cellobiohydrolase ii [cbh], and aspergillus aculeatus β-glucosidase i [bg]) was constructed by yeast cell-surface engineering. the eg used in this study consists of a family 1 carbohydrate-binding module (cbm) and a catalytic module. a comparison with family 1 cbms revealed conserved amino acid residues and flexibl ... | 2012 | 23092441 |
| cellulosic ethanol production by combination of cellulase-displaying yeast cells. | as an effort to find suitable endoglucanases to generate cellulolytic yeast strains, two fungal endoglucanases, thermoascus aurantiacus egi and trichoderma reesei egii, and two bacterial endoglucanases, clostridium thermocellum cela and celd, were expressed on the yeast surface, and their surface expression levels, ph- and temperature-dependent enzyme activities, and substrate specificities were analyzed. t. aurantiacus egi showed similar patterns of ph- and temperature-dependent activities to t ... | 2012 | 23040393 |
| application of endo-β-1,4,d-mannanase and cellulase for the release of mannooligosaccharides from steam-pretreated spent coffee ground. | spent coffee ground (scg), a present waste stream from instant coffee production, represents a potential feedstock for mannooligosaccharides (mos) production. mos can be used in nutraceutical products for humans/animals or added to instant coffee, increasing process yield and improving product health properties. the scg was evaluated for mos production by steam pretreatment and enzymatic hydrolysis with a recombinant mannanase and a commercial cellulase cocktail (acremonium, bioshigen co. ltd, j ... | 2014 | 24557953 |
| utilization of recombinant trichoderma reesei expressing aspergillus aculeatus β-glucosidase i (jn11) for a more economical production of ethanol from lignocellulosic biomass. | the capacity of trichoderma reesei cellulase to degrade lignocellulosic biomass has been enhanced by the construction of a recombinant t. reesei strain expressing aspergillus aculeatus β-glucosidase i. we have confirmed highly efficient ethanol production from converge-milled japanese cedar by recombinant t. reesei expressing a. aculeatus β-glucosidase i (jn11). we investigated the ethanol productivity of jn11 and compared it with the cocktail enzyme t. reesei pc-3-7 with reinforced cellobiase a ... | 2015 | 26026380 |
| identification of cadmium-resistant fungi related to cd transportation in bermudagrass [cynodon dactylon (l.) pers]. | phytoremediation utilizing plants and microbes has been increasingly adopted as a green technology for cleaning up heavy metal polluted soils. cd polluted soil and native bermudagrass from liuyang and zhuzhou in hunan province of china were collected to investigate microbial diversity and isolate cd resistant fungi, and then to determine the effect of cd resistant fungi on cd tolerance and transportation of bermudagrass. the functional diversity of microorganisms was evaluated using the biolog e ... | 2014 | 25461949 |
| production of high galacto-oligosaccharides by pectinex ultra sp-l: optimization of reaction conditions and immobilization on glyoxyl-functionalized silica. | a rational optimization for the synthesis of galacto-oligosaccharides (gos) from lactose catalyzed by β-galactosidase from aspergillus aculeatus, included in the commercial product pectinex ultra sp-l, has been performed by using experimental design and surface response methodology. this accurate tool optimized empirical production of the most desired high-gos (tri-gos and tetra-gos) up to 16.4% under the following reaction conditions: 59 °c, 4 u/ml free enzyme concentration, ph 6.5, 250 g/l ini ... | 2017 | 28176525 |
| identification and functional characterization of a fructooligosaccharides-forming enzyme from aspergillus aculeatus. | although fructosyltransferases from aspergillus aculeatus have received a considerable interest for the prebiotics industry, their amino acid sequences and structural features remain unknown. this study sequenced and characterized a fructosyltransferase from a. aculeatus (acft) isolated by heat treatment of pectinex ultra sp-l. the acft enzyme showed two isoforms, low-glycosylated acft1 and high-glycosylated acft2 forms, with similar optimum activity at 60 °c. the purified heat-resistant acft1 a ... | 2016 | 26857855 |
| biodegradation of 1,2,3,4-tetrachlorodibenzo-p-dioxin in liquid broth by brown-rot fungi. | dioxins are a class of extremely hazardous molecules that might pose a threat to the environment. this work evaluated the microbial degradation of 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-tcdd), in liquid broth using three brown-rot fungi and one white-rot fungi as control. a fast and reliable extraction method with recoveries of over 98% together with a validated gc-ms method was developed, and applied to quantify 1,2,3,4-tcdd in liquid broth, mycelia and reaction flask, with detection limi ... | 2013 | 24080442 |
| investigation of a 6-msa synthase gene cluster in aspergillus aculeatus reveals 6-msa-derived aculinic acid, aculins a-b and epi-aculin a. | aspergillus aculeatus, a filamentous fungus belonging to the aspergillus clade nigri, is an industrial workhorse in enzyme production. recently we reported a number of secondary metabolites from this fungus; however, its genetic potential for the production of secondary metabolites is vast. in this study we identified a 6-methylsalicylic acid (6-msa) synthase from a. aculeatus, and verified its functionality by episomal expression in a. aculeatus and heterologous expression in a. nidulans. feedi ... | 2015 | 26374386 |
| xlnr-independent signaling pathway regulates both cellulase and xylanase genes in response to cellobiose in aspergillus aculeatus. | the expression levels of the cellulase and xylanase genes between the host strain and an xlnr disruptant were compared by quantitative rt-pcr (qpcr) to identify the genes controlled by xlnr-independent signaling pathway. the cellulose induction of the fi-carboxymethyl cellulase (cmc1) and fib-xylanase (xynib) genes was controlled by xlnr; in contrast, the cellulose induction of the fiii-avicelase (cbhi), fii-carboxymethyl cellulase (cmc2), and fia-xylanase (xynia) genes was controlled by an xlnr ... | 2012 | 22371227 |
| characterization of aspergillus aculeatus β-glucosidase 1 accelerating cellulose hydrolysis with trichoderma cellulase system. | aspergillus aculeatus β-glucosidase 1 (aabgl1), which promotes cellulose hydrolysis by trichoderma cellulase system, was characterized and compared some properties to a commercially supplied orthologue in a. niger (anbgl) to elucidate advantages of recombinant aabgl1 (raabgl1) for synergistic effect on trichoderma enzymes. steady-state kinetic studies revealed that raabgl1 showed high catalytic efficiency towards β-linked glucooligosaccharides. up to a degree of polymerization (dp) 3, raabgl1 pr ... | 2015 | 25642400 |
| unravelling the nanostructure of strawberry fruit pectins by endo-polygalacturonase digestion and atomic force microscopy. | pectins analysed by afm are visualized as individual chains, branched or unbranched, and aggregates. to investigate the nature of these structures, sodium carbonate soluble pectins from strawberry fruits were digested with endo-polygalacturonase m2 from aspergillus aculeatus and visualized by afm. a gradual decrease in the length of chains was observed as result of the treatment, reaching a minimum ln value of 22nm. the branches were not visible after 2h of enzymatic incubation. the size of comp ... | 0 | 28159266 |
| simultaneous saccharification and viscosity reduction of cassava pulp using a multi-component starch- and cell-wall degrading enzyme for bioethanol production. | in this study, an efficient ethanol production process using simultaneous saccharification and viscosity reduction of raw cassava pulp with no prior high temperature pre-gelatinization/liquefaction step was developed using a crude starch- and cell wall-degrading enzyme preparation from aspergillus aculeatus bcc17849. proteomic analysis revealed that the enzyme comprised a complex mixture of endo- and exo-acting amylases, cellulases, xylanases, and pectina ses belonging to various glycosyl hydrol ... | 2017 | 28868217 |
| characterization of mechanisms underlying degradation of sclerotia of sclerotinia sclerotiorum by aspergillus aculeatus asp-4 using a combined qrt-pcr and proteomic approach. | the biological control agent aspergillus aculeatus asp-4 colonizes and degrades sclerotia of sclerotinia sclerotiorum resulting in reduced germination and disease caused by this important plant pathogen. molecular mechanisms of mycoparasites underlying colonization, degradation, and reduction of germination of sclerotia of this and other important plant pathogens remain poorly understood. | 2017 | 28859614 |
| does osmotic stress affect natural product expression in fungi? | the discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. however, most of the reported marine-derived species appear to be terrestrial in origin yet at the same time, facultatively halo- or osmotolerant. an unanswered question regarding the apparent chemical productivity of marine-derived fungi is whether the common practice of fermenting strains in seawa ... | 2017 | 28805714 |
| metabolomic tools to assess the chemistry and bioactivity of endophytic aspergillus strain. | endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology that may find applications as pharmaceutical and agrochemical drugs. in this study, a combination of metabolomics and bioactivity-guided approaches were employed to isolate anticancer secondary metabolites from an endophytic aspergillus aculeatus. the endophyte was isolated from the egyptian medicinal plant terminalia laxiflora and identified using molecular biological methods. metabolomics an ... | 2017 | 28672096 |
| dipeptidyl peptidase iv is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in aspergillus aculeatus. | we screened for factors involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes from approximately 12,000 aspergillus aculeatus t-dna insertion mutants harboring a transcriptional fusion between the fiii-avicelase gene (cbhi) promoter and the orotidine 5'-monophosphate decarboxylase gene. analysis of 5-fluoroorodic acid (5-foa) sensitivity, cellulose utilization, and cbhi expression of the mutants revealed that a mutant harboring t-dna at the dipeptidyl peptida ... | 2017 | 28290772 |
| effects of cadmium-resistant fungi aspergillus aculeatus on metabolic profiles of bermudagrass [cynodondactylon (l.)pers.] under cd stress. | plants' tolerance to heavy metal stress may be induced by the exploitation of microbes. the objectives of this study were to investigate the effect of cadmium (cd)-resistant fungus, aspergillus aculeatus, on tolerance to cd and alteration of metabolites in bermudagrass under cd stress, and identify the predominant metabolites associated with cd tolerance. two genotypes of bermudagrass with contrasting cd tolerance (cd-sensitive 'wb92' and cd-tolerant 'wb242') were exposed to 0, 50, 150 and 250 m ... | 2017 | 28273510 |
| secalonic acid- f inhibited cell growth more effectively than 5-fluorouracil on hepatocellular carcinoma in vitro and in vivo. | hepatocellular carcinoma (hcc), one of the most common types of liver cancer, could be treated with 5-fluorouracil (5-fu). due to its side effects, 5-fu is more often used as the co-administration drug in clinical practice. secalonic acid-f (saf), isolated from a fungal strain identified in our lab as aspergillus aculeatus, showed potent biological activities. the goal of this study was to evaluate the inhibitory effects of saf on hepatocellular carcinoma and to compare it with that of 5-fu. res ... | 2017 | 28253713 |
| heterologous expression of aspergillus aculeatus endo-polygalacturonase in pichia pastoris by high cell density fermentation and its application in textile scouring. | removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. in this study, an endo-polygalacturonase (endopg) from aspergillus aculeatus produced in pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation. | 2017 | 28209146 |
| amelioration of salt stress on bermudagrass by the fungus aspergillus aculeatus. | there is considerable evidence that plant abiotic-stress tolerance can be evoked by the exploitation of a globally abundant microbe. a. aculeatus, which was initially isolated from the rhizosphere of bermudagrass, has been shown to increase heavy metal tolerance in turfgrasses. here, we report on the potential of a. aculeatus to induce tolerance to salt stress in bermudagrass. physiological markers for salt stress, such as plant growth rate, lipid peroxidation, photosynthesis, and ionic homeosta ... | 2017 | 28134574 |
| structure and antitumor activity of the extracellular polysaccharides from aspergillus aculeatus via apoptosis and cell cycle arrest. | two extracellular polysaccharides, designated as wpa and wpb, were isolated from the fungus aspergillus aculeatus using q-sepharose fast flow and sephacryl s-300 column chromatography. wpa composed of mannose and galactose in a molar ratio of 3.9:1.0, and wpb mainly contained mannose. the molecular weight of wpa and wpb was about 28.1 kda and 21.0 kda, respectively. on the basis of methylation and nmr analysis, the possible main chain of wpa was [→5)-β-d-galf-(1 → 2,6)-α-d-manp(1→], and wpb was ... | 2016 | 27557923 |
| crystal structure and genetic modifications of fi-cmcase from aspergillus aculeatus f-50. | cellulose is the major component of the plant cell wall and the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in many commercial applications. endo-1,4-β-d-glucanase (ec 3.2.1.4; endoglucanase), which catalyzes the random hydrolysis of 1,4-β-glycosidic bonds of the cellulose main chain to cleave cellulose into smaller fragments, is the key cellulolytic enzyme. an endoglucanase isolated from aspergillus aculeatus f-50 (fi-cmcase), which is classified ... | 2016 | 27470581 |
| site-saturation mutagenesis for β-glucosidase 1 from aspergillus aculeatus to accelerate the saccharification of alkaline-pretreated bagasse. | aspergillus aculeatus β-glucosidase 1 (aabgl1) is one of the best cellobiose hydrolytic enzymes without transglycosylation products, among β-glucosidase from various origins, for use in cellulosic biomass conversion with trichoderma cellulases. however, in our previous report, it was demonstrated that aabgl1 has lower catalytic efficiency toward cellobiose, which is a major end product from cellulosic biomasses by trichoderma reesei cellulases, than do gentiobiose and laminaribiose. thus, we exp ... | 2016 | 27444432 |
| optimization of process parameters for enhanced production of jamun juice using pectinase (aspergillus aculeatus) enzyme and its characterization. | jamun fruit comprises of seed and thick pulp. the pectin-protein bond of the thick pulp creates difficulty in making juice. clear jamun juice is not available in the market, so there is a need for extraction of juice with maximum yield. the goal of this research is to obtain high yield of clarified juice with the help of pectinase (aspergillus aculeatus) enzyme. the study was conducted at different enzyme concentration (0.01-0.1%), time duration (40-120 min), and temperature (30-50 °c). various ... | 2016 | 28330313 |
| a high performance trichoderma reesei strain that reveals the importance of xylanase iii in cellulosic biomass conversion. | the ability of the trichoderma reesei x3ab1strain enzyme preparations to convert cellulosic biomass into fermentable sugars is enhanced by the replacement of xyn3 by aspergillus aculeatus β-glucosidase 1 gene (aabg1), as shown in our previous study. however, subsequent experiments using t. reesei extracts supplemented with the glycoside hydrolase (gh) family 10 xylanase iii (xyn iii) and gh family 11 xyn ii showed increased conversion of alkaline treated cellulosic biomass, which is rich in xyla ... | 2016 | 26672453 |
| synergistic action of recombinant accessory hemicellulolytic and pectinolytic enzymes to trichoderma reesei cellulase on rice straw degradation. | synergism between core cellulases and accessory hydrolytic/non-hydrolytic enzymes is the basis of efficient hydrolysis of lignocelluloses. in this study, the synergistic action of three recombinant accessory enzymes, namely gh62 α-l-arabinofuranosidase (ara), ce8 pectin esterase (pet), and gh10 endo-1,4-beta-xylanase (xyl) from aspergillus aculeatus expressed in pichia pastoris to a commercial trichoderma reesei cellulase (accellerase® 1500; acr) on hydrolysis of alkaline pretreated rice straw w ... | 2015 | 26433794 |
| a crispr-cas9 system for genetic engineering of filamentous fungi. | the number of fully sequenced fungal genomes is rapidly increasing. since genetic tools are poorly developed for most filamentous fungi, it is currently difficult to employ genetic engineering for understanding the biology of these fungi and to fully exploit them industrially. for that reason there is a demand for developing versatile methods that can be used to genetically manipulate non-model filamentous fungi. to facilitate this, we have developed a crispr-cas9 based system adapted for use in ... | 2015 | 26177455 |
| crystallization and preliminary x-ray diffraction analysis of an endo-1,4-β-d-glucanase from aspergillus aculeatus f-50. | cellulose is the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in a wide variety of industries. endo-1,4-β-d-glucanase (ec 3.2.1.4; endoglucanase), which can catalyze the random hydrolysis of β-1,4-glycosidic bonds to cleave cellulose into smaller fragments, is a key cellulolytic enzyme. an endoglucanase isolated from aspergillus aculeatus f-50 (fi-cmcase) that was classified into glycoside hydrolase family 12 has been found to be effectively expres ... | 2015 | 25849498 |
| asperaculanes a and b, two sesquiterpenoids from the fungus aspergillus aculeatus. | six sesquiterpenoids 1-6, including two new ones, an ent-daucane-type sesquiterpenoid, asperaculane a (1), and a nordaucane one, asperaculane b (2), and four known nordaucane derivatives, aculenes a-d 3-6, together with the known secalonic acid d (7), were isolated from a fermentation culture of the fungus aspergillus aculeatus. their structures and absolute configurations were established by analyses of their spectroscopic data, including 1d and 2d-nmr spectra, hr-esims, electronic circular dic ... | 2014 | 25547729 |
| effects of clbr overexpression on enzyme production in aspergillus aculeatus vary depending on the cellulosic biomass-degrading enzyme species. | clbr is a zn(ii)2cys6 transcriptional activator that controls the expression of cellulase-related genes in response to avicel and cellobiose in aspergillus aculeatus. a clbr-overexpressing strain (clbr-oe) that expresses the clbr gene at levels sevenfold higher than the control strain sustainably produced xylanolytic and cellulolytic activities during 10-day cultivation of a. aculeatus, enabling synchronization of xylanolytic and cellulolytic activities at a maximum level. however, clbr overexpr ... | 2015 | 25410617 |
| nickel oxide nanoparticles film produced by dead biomass of filamentous fungus. | the synthesis of nickel oxide nanoparticles in film form using dead biomass of the filamentous fungus aspergillus aculeatus as reducing agent represents an environmentally friendly nanotechnological innovation. the optimal conditions and the capacity of dead biomass to uptake and produce nanoparticles were evaluated by analyzing the biosorption of nickel by the fungus. the structural characteristics of the film-forming nickel oxide nanoparticles were analyzed by scanning electron microscopy (sem ... | 2014 | 25228324 |
| [biological conversion of stevioside to steviol by aspergillus aculeatus and the purification of rebaudioside a]. | the purpose of this research was to apply aspergillus aculeatus solid fermentation extracts to convert stevioside and rebaudioside c, followed by identifying and purifying the new conversion product. | 2014 | 24783855 |
| a new steroid with long cross-conjugation structure from the marine-derived fungus aspergillus aculeatus. | a new steroid with a long cross-conjugation structure, 15a-hydroxy-(22e, 24r)-ergosta-3, 5, 8 (14), 22-tetraen-7-one (1), was isolated from the marine-derived fungus aspergillus aculeatus. its structure was established by the extensive spectroscopic analyses, and its cytotoxicities against p388, hl-60, and pc-3 cell lines were measured in vitro. | 2014 | 24783508 |
| fructooligosaccharides synthesis by highly stable immobilized β-fructofuranosidase from aspergillus aculeatus. | the enzymatic synthesis of fructooligosaccharides (fos) was carried out using a partially purified β-fructofuranosidase from the commercial enzyme preparation viscozyme l. partial purification of β-fructofuranosidase from viscozyme l was done by batch adsorption using ion-exchange resin deae-sepharose, showing a 6-fold increase in specific activity. the biocatalyst was then covalently immobilized on glutaraldehyde-activated chitosan particles. thermal stability of the biocatalyst was evaluated a ... | 2014 | 24528719 |
| dietary sugars analysis: quantification of fructooligossacharides during fermentation by hplc-ri method. | in this work, a simple chromatographic method is proposed and in-house validated for the quantification of total and individual fructooligossacharides (e.g., 1-kestose, nystose, and 1(f)-fructofuranosylnystose). it was shown that a high-performance liquid chromatography with refractive index detector could be used to monitor the dynamic of fructooligossacharides production via sucrose fermentation using aspergillus aculeatus. this analytical technique may be easily implemented at laboratorial or ... | 2014 | 25988114 |
| the characterisation of xyloglucanase inhibitors from humulus lupulus. | phytopathogenic fungi secrete a powerful arsenal of enzymes that are potentially active against each polysaccharide component of the plant cell wall. to defend themselves, plants synthetise a variety of molecules that inhibit the activity of cell wall-degrading enzymes. xyloglucan-specific endoglucanase inhibitor proteins (xegips) act specifically against the members of fungal glycoside hydrolase family 12 (gh12 in the cazy database). in the present study, we describe the identification of three ... | 2013 | 23561301 |
| crystal structures of glycoside hydrolase family 3 β-glucosidase 1 from aspergillus aculeatus. | gh3 (glycoside hydrolase family 3) bgls (β-glucosidases) from filamentous fungi have been widely and commercially used for the supplementation of cellulases. aabgl1 (aspergillus aculeatus bgl1) belongs to the gh3 and shows high activity towards cellooligosaccharides up to high degree of polymerization. in the present study we determined the crystal structure of aabgl1. in addition to the substrate-free structure, the structures of complexes with glucose and various inhibitors were determined. th ... | 2013 | 23537284 |
| hydrolysis of various thai agricultural biomasses using the crude enzyme from aspergillus aculeatus iizuka fr60 isolated from soil. | in this study, forty-two fungi from soil were isolated and tested for their carboxymethyl cellulase (cmcase) and xylanase activities. from all isolates, the fungal isolate fr60, which was identified as aspergillus aculeatus iizuka, showed high activities in both cmcase and xylanase with 517 mu/mg protein and 550 mu/mg protein, respectively. the crude enzyme from a. aculeatus iizuka fr60 could hydrolyze several agricultural residues such as corncob, and sweet sorghum leaf and stalk at comparable ... | 2012 | 24031852 |
| mass production of rubusoside using a novel stevioside-specific β-glucosidase from aspergillus aculeatus. | rubusoside (r) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. however, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable r-production method. a stevioside (st)-specific β-glucosidase (ssgase) was selected and purified 7-fold from aspergillus aculeatus viscozyme l by a two-step column chromatography procedure. the 79 kda protein was stable f ... | 2012 | 22530920 |
| purification and characterization of a naringinase from aspergillus aculeatus jmudb058. | a naringinase from aspergillus aculeatus jmudb058 was purified, identified, and characterized. this naringinase had a molecular mass (mw) of 348 kda and contained four subunits with mws of 100, 95, 84, and 69 kda. mass spectrometric analysis revealed that the three larger subunits were β-d-glucosidases and that the smallest subunit was an α-l-rhamnosidase. the naringinase and its α-l-rhamnosidase and β-d-glucosidase subunits all had optimal activities at approximately ph 4 and 50 °c, and they we ... | 2013 | 23289582 |
| reversible impairment of the ku80 gene by a recyclable marker in aspergillus aculeatus. | auxotrophic mutants of aspergillus can be isolated in the presence of counter-selective compounds, but the process is laborious. we developed a method to enable reversible impairment of the ku80 gene (aaku80) in the imperfect fungus aspergillus aculeatus. aaku80 was replaced with a selection marker, orotidine 5'-phosphate decarboxylase (pyrg), followed by excision of pyrg between direct repeats (dr) to yield the aaku80 deletion mutant (mr12). the gene-targeting efficiency at the ornithine carbam ... | 2013 | 23311774 |