| drug-induced alterations in the ultrastructural organization of histoplasma capsulatum and blastomyces dermatitidis. | | 1972 | 4119289 |
| ultrastructural studies on the cleistothecium of ajellomyces dermatitidis. | | 1973 | 4127194 |
| the fine structure of the microconidium of blastomyces dermatitidis. | | 1974 | 4134814 |
| isolation of skin test-active preparations from yeast-phase cells of blastomyces dermatitidis. | cell wall and cytoplasmic fractions were isolated from mechanically disrupted yeast-phase cells of blastomyces dermatitidis in an effort to obtain a reliable skin-test antigen. the biological activities of these fractions were compared with those of two blastomycin preparations. the cytoplasmic antigens exhibited a low index of specificity yet exceeded the specificities of the blastomycins. the skin test-active component(s) of the cytoplasmic material had a molecular weight between 10,000 and 30 ... | 1974 | 4135392 |
| yeast- and mycelial-phase antigens of blastomyces dermatitidis: comparison using disc gel electrophoresis. | the disc gel electrophoretic patterns obtained with skin test-active (mycelial and yeast) antigens of blastomyces dermatitidis were compared. as would be expected, the blastomycins (mycelial-phase) and the cytoplasmic ultrafiltrates (yeast-phase) were heterogeneous mixtures containing proteins, glycoproteins, lipoproteins, and carbohydrates. the skin test-active cytoplasmic ultrafiltrates and the blastomycins contained glycoproteins that had similar r(f) values which allows the possibility that ... | 1974 | 4135463 |
| osteomyelitis caused by concurrent infection with mycobacterium tuberculosis and blastomyces dermatitidis. | | 1974 | 4204542 |
| extracellular enzymes of blastomyces dermatitidis. | | 1969 | 4313407 |
| optimal combination and concentration of antibiotics in media for isolation of pathogenic fungi and nocardia asteroides. | strains of blastomyces dermatitidis, sporothrix schenckii, histoplasma capsulatum, cryptococcus neoformans, nocardia asteroides, and coccidioides immitis were tested for in vitro susceptibility to polymyxin, gentamicin, kanamycin, chloramphenicol, and neomycin at concentrations of 1, 2, 4, 8, and 16 mug/ml. polymyxin was the most inhibitory and gentamicin was the least inhibitory of the five antibiotics. two histoplasma mycelial strains were partially inhibited by 2 and 8 mug of gentamicin per m ... | 1971 | 4323185 |
| optimal combination and concentration of antibiotics in media for isolation of pathogenic fungi and nocardia asteroides. | | 1971 | 4323647 |
| toxicity of blastomyces dermatitidis. | | 1971 | 4331601 |
| passive transfer of delayed hypersensitivity to blastomyces dermatitidis between mice. | this study further characterized the delayed hypersensitivity state induced in animals by blastomyces dermatitidis exposure. passive transfer of delayed hypersensitivity by transfer of cells and inhibition of migration of peritoneal exudate cells were studied, using sensitized mice of two inbred strains. donor mice were subcutaneously inoculated with viable b. dermatitidis yeast cells. after 15 days, spleen cells or serum from these animals were injected intravenously into normal recipients of t ... | 1974 | 4426704 |
| observations of a naturally-occurring lattice array of ribosomes in ultrathin section of ajellomyces dermatitidis. | | 1974 | 4437590 |
| the uptake and incorporation of leucine and cystine by the mycelial and yeastlike phases of blastomyces dermatitidis. | | 1974 | 4444728 |
| a new hypothesis on the epidemiology of blastomycosis and the ecology of blastomyces dermatitidis. | | 1973 | 4516564 |
| morphological conversion of blastomyces dermatitidis in tap-water. | strains of b. dermatitidis not only survived but multiplied in sterile water from 2 separate locations in the usa. regardless of the growth phase at which the water was inoculated, the resultant growth phase that predominated was temperature-dependent. at 37 degrees c the organism grew primarily as a yeast; at room temperature, growth was mycelial. complex media may be unnecessary for converting this organism to the yeast phase, and water should be further investigated as a substrate, from both ... | 1974 | 4549493 |
| composition of the alkali resistant cell wall material of dimorphic blastomyces dermatitidis. | | 1972 | 4557874 |
| exogenous mycotic keratitis caused by blastomyces dermatitidis. | | 1973 | 4574747 |
| skin testing of guinea pigs and footpad testing of mice with a new antigen for detecting delayed hypersensitivity to cryptococcus neoformans. | this study was undertaken to evaluate the potential of a cryptococcal culture filtrate antigen, cryptococcin c184, for detecting delayed hypersensitivity in cryptococcus neoformans-injected animals. the antigen was tested on guinea pigs which had received saline or c. neoformans and on animals sensitized to histoplasma capsulatum, blastomyces dermatitidis, candida albicans, or sporothrix schenckii. a delayed-type hypersensitivity response was elicited by cryptococcin c184 in c. neoformans-inject ... | 1974 | 4593343 |
| antigenic relationship between american and african isolates of blastomyces dermatitidis as determined by immunofluorescence. | the antigenic relationship between american and african isolates of blastomyces dermatitidis was investigated through the use of the fluorescent antibody technique. preliminary results suggest that the american and african isolates studies are not antigenically identical. the american isolates share antigens with the african ones; however, it appears that they also possess distinct antigens. additional studies are necessary to confirm these findings and to draw any conclusion whether the african ... | 1974 | 4596385 |
| effect of chitinase complex on the antigenicity and chemistry of yeast-form cell walls and other fractions of histoplasma capsulatum and blastomyces dermatitidis. | | 1974 | 4604380 |
| cell wall composition of two strains of blastomyces dermatitidis exhibiting differences in virulence for mice. | cell walls isolated from two strains of blastomyces dermatitidis were examined. whereas strain ga-1 was practically avirulent for mice, strain kl-1 produced death by 21 days in 50% of the mice inoculated. analyses of the trypsin-treated cell walls of the two strains revealed a higher chitin and protein content in strain kl-1, whereas a higher polysaccharide content was observed in the cell walls of strain ga-1. extraction of the walls with 1 n naoh revealed a threefold difference in the amount o ... | 1972 | 4636784 |
| sexual stage of histoplasma capsulatum. | twelve primary subcultures of histoplasma capsulatum, paired in all possible combinations on agar containing yeast extract and alphacel, produced fertile cleistothecia, resembling those of ajellomyces dermatitidis (blastomyces dermatitidis). | 1972 | 4651892 |
| cerebellar abscess due to blastomyces dermatitidis. | a case of cerebellar abscess due to blastomyces dermatitidis is reported and the literature of central nervous system blastomycosis is reviewed. the case is of interest for two reasons: 1. no obvious site of primary blastomycotic infection was found, despite an extensive search. 2. the focal blastomycotic involvement of the cns was not associated with meningitis. only two other such cases are explicitly reported in the literature. | 1972 | 4665097 |
| ultrastructural comparison of coils and ascospores of emmonsiella capsulata and ajellomyces dermatitidis. | | 1973 | 4686217 |
| role of delayed hypersensitivity in blastomycosis of mice. | c57bl/6j mice rendered hypersensitive to blastomyces dermatitidis were protected from the lethal effects of a blastomyces infection. this protection was observed following a lethal intraperitoneal challenge with viable cells 15 days after subcutaneous inoculation with 3.9 x 10(4) viable cells. delayed hypersensitivity was induced in c57 mice by two injections of merthiolate-killed cells in adjuvant or by a single injection of viable cells. development of hypersensitivity was determined at approp ... | 1973 | 4713689 |
| blastomyces dermatitidis in cytologic preparations. | | 1973 | 4715963 |
| amphotericin pharmacophobia. | five cases are described in which fear of the possibly hazardous effects of giving amphotericin to patients with kidney disease resulted in death from progressive infection by an amphotericin-sensitive fungus (cryptococcus neoformans in three cases, blastomyces dermatitidis in one case, and histoplasma capsulatum in one case). | 1973 | 4758449 |
| soil streptomycetes and bacteria related to lysis of blastomyces dermatitidis. | | 1973 | 4765981 |
| geographic distribution of "plus" and "minus" isolates of blastomyces (ajellomyces) dermatitidis in north america. | | 1973 | 4780929 |
| histologic reactions to cell walls of an avirulent and a virulent strain of blastomyces dermatitidis. | | 1974 | 4810940 |
| development and use of a polyvalent conjugate to differentiate histoplasma capsulatum and histoplasma duboisii from other pathogens. | previous investigations have demonstrated the existence of five histoplasma capsulatum serotypes. available specific fluorescent-antibody reagents stain only four of the five serotypes. antibodies produced against the most complete h. capsulatum serotype were labeled with fluorescein isothiocyanate to develop a reagent specific for h. capsulatum that was reactive with all the known serotypes. the unadsorbed reagent not only stained all the h. capsulatum serotypes, but it also stained cultures of ... | 1968 | 4869210 |
| in vitro antifungal activity of clotrimazole (bay b 5097). | the in vitro antifungal activity of clotrimazole (bay b 5097) was compared with those of amphotericin b, griseofulvin, nystatin, and pyrrolnitrin. the inhibitory activity of clotrimazole against most systemic pathogens was comparable to that of amphotericin b; minimal inhibitory concentrations of the two drugs for blastomyces dermatitidis, histoplasma capsulatum, sporothrix schenckii, cryptococcus neoformans, and coccidioides immitis were in the range of 0.20 to 3.13 and 0.10 to 6.25 mug/ml, res ... | 1971 | 4949484 |
| serological response of rhesus monkeys to histoplasma, blastomyces, and coccidioides antigens. | fifteen adult rhesus monkeys were inoculated with nonviable histoplasma capsulatum, blastomyces dermatitidis, or coccidioides immitis. antibody assays were made periodically during a 2-year period by use of a complement-fixation (cf) test employing four antigens and a latex-agglutination test. selected sera were also studied in an immunodiffusion test and a coccidioidin-precipitin test. the serological patterns obtained with the anti-histoplasma, anti-blastomyces, and anti-coccidioides monkey se ... | 1967 | 4961413 |
| cytolytic plasma factor in experimental coccidioidomycosis. | peripheral blood leukocytes from guinea pigs infected with coccidioides immitis 5 weeks previously were lysed significantly when incubated at 37 c for 3.5 hr with a concentrated c. immitis culture filtrate, but not with a concentrated aspergillus fumigatus or blastomyces dermatitidis culture filtrate. mononuclear leukocytes and presumably granulocytes were both lysed. characterization of the factor responsible for this leukocytolysis reaction revealed that it was contained in plasma and serum bu ... | 1967 | 4963788 |
| protein constituents of cell walls of the dimorphic phases of blastomyces dermatitidis. | | 1972 | 5022061 |
| deoxyribonucleic acid base composition of the yeastlike and mycelial phases of histoplasma capsulatum and blastomyces dermatitidis. | the base composition in moles percent guanine plus cytosine (%gc) of both nuclear and mitochondrial deoxyribonucleic acid (dna) isolated from the yeastlike and mycelial phases of the dimorphic fungal pathogens histoplasma capsulatum and blastomyces dermatitidis was determined by techniques of thermal denaturation and cscl buoyant density gradient equilibrium centrifugation. the mean observed values for gc content of nuclear dna from h. capsulatum and b. dermatitidis were 47.3 and 48.2%, respecti ... | 1972 | 5053471 |
| frequency of isolation of histoplasma capsulatum and blastomyces dermatitidis from dogs in kentucky. | | 1972 | 5058530 |
| the readily extracted lipids of histoplasma capsulatum and blastomyces dermatitidis. | | 1971 | 5089693 |
| monosaccharide and chitin content of cell walls of histoplasma capsulatum and blastomyces dermatitidis. | cell walls of histoplasma capsulatum and blastomyces dermatitidis, obtained by mechanical breakage of yeast- and mycelial-phase cultures, were lipid-extracted and then fractionated with ethylenediamine. unextracted cell walls, lipid-extracted cell walls, and the three fractions resulting from ethylenediamine treatment were examined for monosaccharide and chitin content. the yeast-phase cell walls of five strains of h. capsulatum fell into two categories, designated chemotypes i and ii, one of wh ... | 1971 | 5095293 |
| genetic analysis on the incompatibility system of ajellomyces dermatitidis. | | 1971 | 5135086 |
| [north american blastomycosis. experimental study of a strain of blastomyces dermatitidis isolated from an african subject]. | | 1971 | 5149996 |
| occurrence of serotypes among histoplasma capsulatum strains. | kaufman, leo (communicable disease center, atlanta, ga.), and sharon blumer. occurrence of serotypes among histoplasma capsulatum strains. j. bacteriol. 91:1434-1439. 1966.-extensive studies with a fluorescent antibody specific for the yeast form of histoplasma capsulatum indicated that, although most strains of h. capsulatum stained intensely, others stained weakly and some did not stain. these observations suggested that antigenic variants of the fungus existed. to test this hypothesis, recipr ... | 1966 | 5326107 |
| the susceptibility of blastomyces dermatitidis to drying. | | 1967 | 5338714 |
| [antigenic study of agents causing deep fungal infections by comparative analysis of culture media. i. histoplasma capsulatum and h. duboisii. relationship with h. farciminosum, gymnoascus demonbreunii, blastomyces dermatitidis and paracoccidioides brasiliensis]. | | 1969 | 5371826 |
| blastomyces dermatitidis meningoencephalitis in a dog. | | 1969 | 5388553 |
| ultrastructural changes during the yeastlike to mycelial-phase conversion of blastomyces dermatitidis and histoplasma capsulatum. | fine details of the sequential anatomical events occurring during yeast to mold morphogenesis of the dimorphic fungal pathogens blastomyces dermatitidis and histoplasma capsulatum as seen in ultrathin sections are described and illustrated by electron micrographs. discrete intracytoplasmic membrane systems intimately associated with the plasma membrane were observed to be formed within 6 to 8 hr after induction of the conversion process. within 12 to 18 hr, an intermediate or transitional cell w ... | 1970 | 5413831 |
| filamentous forms of blastomyces dermatitidis in mouse lung. light and electron microscopy. | | 1970 | 5418299 |
| comparison of infection of laboratory animals with blastomyces dermatitidis using different routes of inoculation. | | 1970 | 5418318 |
| electronmicroscopy of self-parasitism by histoplasma capsulatum and blastomyces dermatitidis. | | 1970 | 5470550 |
| susceptibility of laboratory animals to blastomyces dermatitidis using different routes of inoculation. | | 1970 | 5496705 |
| comparison of macrocomplement and microcomplement fixation techniques used in fungus serology. | a comparative evaluation was performed on the micro- and macrocomplement fixation (cf) tests that are used as standard procedures in the serodiagnosis of blastomycosis, coccidioidomycosis, and histoplasmosis. tests with 937 sera from suspected and culturally proven cases of these diseases against yeast-form antigens of blastomyces dermatitidis and histoplasma capsulatum and against the soluble antigens, coccidioidin and histoplasmin, revealed that the microtiters were within +/- 1 dilution of th ... | 1970 | 5498606 |
| evaluation of systemic antifungal agents in x-irradiated mice. | the effect of x irradiation on the survival time of animals experimentally infected with pathogenic fungi was studied, and the activity of antifungal agents in pre-irradiated hosts was evaluated. a 24-hr preinfection dose of x irradiation decreased the survival time of mice infected with cryptococcus neoformans and histoplasma capsulatum to a greater extent than candida albicans or blastomyces dermatitidis infections. exposure to 400 r caused a significant reduction in the variation (s(2)) survi ... | 1970 | 5498613 |
| blastomycosis-epidemiology and biology of its etiologic agent ajellomyces dermatitidis. | | 1970 | 5535368 |
| polymorphism of malate dehydrogenase in cytoplasmic and mitochondrial fractions of blastomyces dermatitidis. | | 1971 | 5553909 |
| cell wall composition of the yeastlike and mycelial forms of blastomyces dermatitidis. | the thermally induced changes in the cell wall polysaccharides of blastomyces dermatitidis strain bd64, which produces a yeastlike form (y form) at 37 c and a mycelial form (m form) at 20 c, were examined. the cell walls of the y and m forms contained 36 and 51% of hexoses, respectively. the m-form cell wall contained glucose, galactose, and mannose in a molar ratio of 1:0.1:0.2. the y-form cell wall contained mainly glucose and a very small amount of galactose and mannose. the glucans of the ce ... | 1971 | 5557599 |
| blastomyces dermatitidis infection in the congo. report of a second autochthonous case. | | 1968 | 5637024 |
| the ascigerous stage of blastomyces dermatitidis. | | 1968 | 5644961 |
| mycelial phase of paracoccidioides brasiliensis and blastomyces dermatitidis: an electron microscope study. | a comparative study of the mycelial phase of paracoccidioides brasiliensis and blastomyces dermatitidis reveals that both fungi are very much alike, containing multiple nuclei and nuclear pores, mitochondria, ribosomes, scarce endoplasmic reticulum, intracytoplasmic membrane systems, glycogen, and vacuoles. shadowed cell walls show fine fibrillar surfaces that contrast with those in the yeast phase. the intracytoplasmic membrane system is continuous with the plasma membrane and is similar to bac ... | 1968 | 5674059 |
| respiratory infection of laboratory animals with conidia of blastomyces dermatitidis. | | 1968 | 5700465 |
| [1st case of blastomycosis due to blastomyces dermatitidis observed in mozambique. cure by amphotericin b]. | | 1968 | 5755458 |
| [blastomycosis caused by blastomyces dermatitidis. its existence in africa]. | | 1968 | 5758395 |
| protoplasts of systemic dimorphic fungal pathogens: histoplasma capsulatum and blastomyces dermatitidis. | | 1969 | 5768613 |
| in vitro and in vivo activity of hamycin against blastomyces dermatitidis. | clinical responses of patients with blastomycosis to treatment with hamycin have been variable. an explanation for this was sought in a series of studies in which in vitro and in vivo susceptibilities to hamycin of five strains of blastomyces dermatitidis were compared. minimal inhibitory concentrations of hamycin for the five strains indicated uniformly high levels of in vitro susceptibility (0.008 to 0.016 mug/ml). in vivo activity was measured in infected mice treated intraperitoneally for a ... | 1969 | 5773006 |
| cell wall composition of the yeast and mycelial forms of paracoccidioides brasiliensis. | isolation and chemical analyses of the cell walls of the yeast (y form) and mycelial forms (m form) of paracoccidioides brasiliensis and blastomyces dermatitidis revealed that their chemical composition is similar and depends on the form. lipids, chitin, glucans, and proteins are the main constituents of the cell walls of both forms of these fungi. there is no significant difference in the amount of lipids (5 to 10%) and glucans (36 to 47%) contained by the two forms. in both fungi, the y form h ... | 1969 | 5776517 |
| systemic antifungal activity of pyrrolnitrin. | the antifungal activity of pyrrolnitrin, previously shown to be effective against superficial infections, was evaluated against experimental systemic mycoses. pyrrolnitrin was inhibitory in vitro at <0.78 to 100 mug/ml to candida albicans, cryptococcus neoformans, blastomyces dermatitidis, sporotrichum schenckii, and histoplasma capsulatum. pyrrolnitrin activity was reduced about 90% in sera. after multiple subcutaneous doses of pyrrolnitrin at 20 mg/kg, activity was recovered in mouse blood and ... | 1969 | 5785951 |
| [fungistatic and fungicidal effect of phenol, lysol and formaldehyde on the fungi coccidioides immitis 7-86, histoplasma capsulatum 6652, and blastomyces dermatitidis 6064]. | | 1969 | 5787564 |
| diagnostic possibilities of utilizing precipitation in agar for the identification of histoplasma capsulatum, coccidioides immitis, blastomyces dermatitidis and paracoccidioides brasiliensis. | | 1966 | 5950913 |
| a comparison of the relative suceptibilities of laboratory animals to infection with the mycelial phase of blastomyces dermatitidis. | | 1966 | 5977761 |
| endogenous mycotic panophthalmitis caused by blastomyces dermatitidis. report of a case and a review of the literature. | | 1967 | 6019015 |
| blastomyces dermatitidis: production of the sexual stage. | growth of five combinations of strains of blastomyces dermatitidis in paired cultures on yeast-extract agar, plus bone meal, yielded the perfect stage for the first time. these cleistothecia, apparently of a new genus, produced eight spored asci. monoascospore cultures developed hyphae and conidia typical of b. dermatitidis. mice were successfully infected with inocula from a monoascospore culture. | 1967 | 6021679 |
| experimental infection of bats (tadarida brasiliensis) with blastomyces dermatitidis. | | 1967 | 6025762 |
| cell wall changes during the budding process of paracoccidioides brasiliensis and blastomyces dermatitidis. | the difference between the budding process of paracoccidioides brasiliensis and blastomyces dermatitidis is reported herein. a characteristic feature in p. brasiliensis is that the optical density of the cell wall increases at the site where budding begins and at the neck of the dividing cell, whereas b. dermatitidis does not undergo this alteration. the neck which is formed between the mother and daughter cell at the site of division is much wider in b. dermatitidis than in p. brasiliensis. the ... | 1967 | 6027991 |
| effect of continuous and intermittent elevated temperatures on the in vitro growth of blastomyces dermatitidis. | | 1967 | 6036224 |
| case report: blastomycosis causing sciatic neuritis. | a patient presented with pain in the right lower back, radiating down the right leg. initial pelvic x-rays did not reveal any lesion. a follow up computerized tomography (ct) scan and technitium scan showed a sharply lytic lesion of the right ilium extending to the right sacroiliac joint. open biopsy revealed a granulomatous inflammation with many budding yeast from consistent with blastomyces dermatitidis. subsequent culture confirmed this identification. there was no other site of fungal infec ... | 1984 | 6088984 |
| in vitro studies with r 51,211 (itraconazole). | the in vitro activity of r 51,211 (itraconazole, accepted generic name; janssen pharmaceutica, beerse, belgium), a new orally active triazole, was compared with those of two existing orally active azoles, ketoconazole and bay n 7133, and a topical agent, ro 14-4767/002. an agar dilution procedure (kimmig agar) was performed with 148 isolates of pathogenic fungi. incubation was at 30 degrees c from 48 h to 7 days. r 51,211 was dissolved in 0.2 n hcl in absolute ethanol, ketoconazole was dissolved ... | 1984 | 6089654 |
| cyclic adenosine 3',5' monophosphate (c-amp) as a factor in phase morphogenesis of blastomyces dermatitidis. | | 1984 | 6090894 |
| new, special stain for histopathological diagnosis of cryptococcosis. | the masson-fontana silver stain for melanin was employed for the differentiation of pathogenic fungal species in human or mouse tissues. the fungi studied were candida albicans, candida tropicalis, candida glabrata (torulopsis glabrata), cryptococcus neoformans, cryptococcus bacillisporus, coccidioides immitis, blastomyces dermatitidis, histoplasma capsulatum, paracoccidioides brasiliensis, sporothrix schenckii, rhizopus rhizopodiformis, and aspergillus fumigatus. the tissue sections stained wit ... | 1981 | 6162857 |
| antigens from histoplasma capsulatum and blastomyces dermatitidis. i. immunological comparative studies from polysaccharide-protein complexes of both fungi. | certain groups of fungi share chemical structures which makes difficult isolation and differentiation of specific antigens by the usual methods of extraction and purification. therefore, we have oriented our studies to the immunological and biochemical characterization of differences and similarities of molecular structures from fungi, etiologic agents of systemic mycoses, hoping to establish criteria for the utilization and handling of these antigens. a deproteinized polysaccharide-protein comp ... | 1982 | 6178974 |
| acid-fastness of fungi in blastomycosis and histoplasmosis. | in 60% of the infections caused by blastomyces dermatitidis and in 47% of the infections caused by histoplasma capsulatum, some of the organisms are acid-fast in tissue sections. treatment with 1n hydrochloric acid at 60 degrees c abolishes the acid-fastness of h capsulatum, which indicates that the acid-fastness may be related to mycolic acid. organisms of b dermatitidis, however, remain acid-fast in 83% of the sections treated with acid. the presence of acid-fastness in small cells can aid in ... | 1982 | 6180697 |
| relationship of progesterone- and estradiol-binding proteins in coccidioides immitis to coccidioidal dissemination in pregnancy. | pregnancy is a major risk factor for coccidioidal dissemination. because rates of coccidioides immitis growth and endospore release are stimulated in vitro by levels of unbound progesterone and 17 beta-estradiol that are achievable, in vivo, in the sera of pregnant women (i.e., 10(-9) to 10(-8) m), a specific-hormone-binding system in c. immitis was sought. fungal cytosols were incubated with tritiated steroids plus or minus radioinert steroids to identify specific binding systems. all five stra ... | 1983 | 6188699 |
| ultrastructural aspects of cytoplasmic ribosomes from histoplasma capsulatum and blastomyces dermatitidis as revealed by heavy metal staining. | cytoplasmic ribosomes were isolated and purified from sonicates of the mycelial and yeastlike growth forms of the pathogenic dimorphic fungi, histoplasma capsulatum and blastomyces dermatitidis. similar ribosomal fractions were prepared from neurospora crassa and saccharomyces cerevisiae. these latter organisms were selected as typical filamentous and yeastlike monophasic fungi, and their ribosomes were used as reference standards. high resolution electron microscopy permitted a comparison of bo ... | 1984 | 6206396 |
| specificity of antigens from pathogenic aspergillus species. i. studies with elisa and immunofluorescence. | studies were made by enzyme linked immunosorbent assay (elisa) and indirect fluorescent antibody (ifa) tests on the reactivities and specificities of 13 antigens prepared from four species of aspergillus against antisera from immunized rabbits and 64 sera from patients with aspergillosis, other systemic mycoses and nocardiosis. although reactions in both serological tests were invariably strongest with homologous antigen: antibody systems, antisera from rabbits immunized with a. fumigatus, blast ... | 1984 | 6209813 |
| hydroxamate siderophore production by opportunistic and systemic fungal pathogens. | it has been suggested that siderophores may function as virulence factors. there have been few studies on production of siderophores by opportunistic and pathogenic fungi. we examined siderophore production by absidia corymbifera, aspergillus niger, rhizopus arrhizus, rhizopus oryzae, blastomyces dermatitidis, histoplasma capsulatum, sporothrix schenickii, candida albicans, and trichophyton mentagrophytes. fungi were cultured at 37 and 27 degrees c in a chemically defined low-iron media (0.2 mic ... | 1983 | 6221998 |
| chronic mucocutaneous candidosis and other superficial and systemic mycoses successfully treated with ketoconazole. | four patients with chronic mucocutaneous candidosis from early infancy were treated successfully with ketoconazole given orally. all thrush lesions were clinically and mycologically cured within a few days of treatment with 100-400 micrograms of ketoconazole daily; skin lesions were cured within a few weeks, and nails were cured after about three months of treatment. delayed cutaneous hypersensitivity to candidin was acquired by the third month. cellular and humoral immunologic responses were re ... | 1980 | 6255540 |
| laboratory and clinical assessment of ketoconazole in deep-seated mycoses. | forty-eight cases of deep mycoses were studied and treated with ketoconazole, each with in vitro evaluation of the minimum inhibitory concentrations (mic) of the causative fungi, in vivo pharmacokinetic, clinical, and mycologic evaluations, several months to two years after the treatment was stopped. excellent results were obtained in six cases of chronic mucocutaneous candidiasis, with restoration of immunologic disturbances; 23 cases of systemic candidiasis, including new aspects of heroin add ... | 1983 | 6295150 |
| molecular aspects of fungal dimorphism. | the approach to this article will be the review of experimental developments achieved in recent years in the fields of biochemistry, molecular biology, ultrastructure, etc. and that have suggested models and hypothesis in order to understand the regulation of the molecular mechanisms involved in fungal differentiation. since fungal dimorphism has been seen by many investigators as a useful model of differentiation in eukaryotic systems, and also because of the prevalence of dimorphism among huma ... | 1984 | 6329600 |
| detection of fungi in cerebrospinal fluid. | with the exception of cryptococcus neoformans, fungi are rarely detected in cerebrospinal fluid obtained from patients having or suspected of having fungal meningitis. a review of the literature reveals that several fungi have been either isolated, observed, or both in cerebrospinal fluid specimens. these fungi include acremonium species, aspergillus amstelodami, a. flavus, a. fumigatus, a. oryzae, a. terreus, blastomyces dermatitidis, candida albicans, c. tropicalis, c. viswanathii, coccidioide ... | 1983 | 6349340 |
| effect of formalin on the survival of systemic fungi in tissue. | stock cultures of blastomyces dermatitidis, candida albicans, cryptococcus neoformans, histoplasma capsulatum and sporothrix schenckii were grown in yeast phase on sabouraud dextrose agar. standardized suspensions (10(8) colony forming units/ml) were made and 1. the organisms treated for various times with 10% formalin/acetate buffer; 2. aliquots injected into the tail veins of swiss-webster mice. minimal fungistatic and fungicidal concentrations of formalin were also determined. five minutes or ... | 1983 | 6350884 |
| antifungal action of rifampin. | rifampin and amphotericin b were found to be synergistic in vitro against saccharomyces cerevisiae, histoplasma capsulatum, and several species of aspergillus, the uptake of rifampin by the fungi was increased in the presence of amphotericin b, and this increase probably formed the basis for the synergistic activity observed. in vivo, the combination proved more effective than either drug alone against experimental infections with h. capsulatum, blastomyces dermatitidis, and aspergillus species. | 1983 | 6356283 |
| effects of culture filtrates of blastomyces dermatitidis on neutrophil locomotion. | a potent chemotactic activity for neutrophils is detectable in liquid culture filtrates of blastomyces dermatitidis. the production of this activity is medium-dependent and culture age-dependent. the highest levels of cytotaxin were produced in filtrates of b. dermatitidis grown in tissue culture medium 199 for three or more weeks. this factor(s) stimulates directed as well as random migration. it functions directly and independently of serum. it is stable at -20 degrees c and 56 degrees c, and ... | 1984 | 6374935 |
| immunological activation of polymorphonuclear neutrophils for fungal killing: studies with murine cells and blastomyces dermatitidis in vitro. | the interaction of elicited murine polymorphonuclear neutrophils (pmn) and the thermally dimorphic fungal pathogen blastomyces dermatitidis in vitro was studied. the pmn elicited intraperitoneally with thioglycollate, in normal mice or mice immune to b dermatitidis, failed to reduce colony forming units (cfu) of b dermatitidis in the inoculum in a 4-hr in vitro assay, even in the presence of 10% fresh immune serum. in contrast, pmn elicited intraperitoneally in immune mice by injection of nonvia ... | 1984 | 6384406 |
| the epidemiology and ecology of blastomycosis, coccidioidomycosis and histoplasmosis. | blastomycosis, coccidioidomycosis and histoplasmosis are the major pulmonary mycoses of humans. each is considered to be a primary pulmonary disease acquired by inhalation of infectious spores from the environment. blastomycosis occurs predominantly in the mississippi river valley and southeastern portions of the u.s.a. clinical cases have also been noted from a few other areas of the world. the etiologic agent, blastomyces dermatitidis, is not readily recoverable from nature, but the disease is ... | 1984 | 6385560 |
| fungal splenic abscess. | nineteen patients with fungal splenic abscesses included 16 with leukemia, one drug user, one patient with cushing's syndrome, and one without predisposing causes. fifteen had persistent fever; six had esophageal or mucocutaneous candidiasis. abdominal pain occurred in seven patients, splenomegaly occurred in six, and left abdominal mass occurred in one. three chest x-ray films showed left pleural effusions; two had elevated left hemidiaphragms, two had left-sided infiltrates, and one was normal ... | 1984 | 6385895 |
| disseminated inoculation blastomycosis in a renal transplant recipient. | we report a case of inoculation blastomycosis with dissemination to the lungs. the patient, a renal allograft recipient, was inoculated accidentally at the veterinarian's office where she worked. a nodular lesion appeared at the inoculation site, accompanied by lymphadenopathy and fever. pulmonary infiltrates developed. blastomyces dermatitidis was identified by both biopsy and culture from both inoculation site and lungs. although not previously reported, inoculation blastomycosis can dissemina ... | 1984 | 6391311 |
| evaluation of a commercial exoantigen test system for the rapid identification of systemic fungal pathogens. | seventy-nine mycelial-form stock cultures of blastomyces dermatitidis, coccidioides immitis, histoplasma capsulatum, and morphologically similar fungi were extracted and tested by using commercial macroimmunodiffusion exoantigen test kits and the centers for disease control (cdc) reference system for identifying fungal isolates. results showed 100% correlation between the two systems. specific exoantigens of c. immitis and h. capsulatum extracted from agar slant cultures (slant extraction method ... | 1983 | 6402920 |
| counterimmunoelectrophoresis (immunoelectroosmosis) and serum electrophoretic pattern in serologic diagnosis of canine blastomycosis. | counterimmunoelectrophoresis (ciep) with blastomyces and histoplasma antigens was used in a serologic study of 181 dogs clinically suspected of having blastomycosis and of 8 dogs with confirmed blastomycosis or histoplasmosis. thirteen of the 181 dogs, positive by ciep, were euthanatized, and the diagnosis was confirmed by cultivation and/or microscopic detection of blastomyces dermatitidis. additional ciep-positive dogs were confirmed by staining of aspirates collected in vivo. radiographic sup ... | 1983 | 6402960 |
| exoantigen tests for the immunoidentification of fungal cultures. | exoantigen tests for the immunoidentification of fungal pathogens are playing a new and significant role in the diagnostic laboratory. properly performed and controlled exoantigen tests lead to rapid, accurate identification of cultures of many fungal pathogens. the tests are particularly valuable in identifying dimorphic pathogens that are difficult to convert or with atypical cultures. we review the value of exoantigen tests for identifying mycelial form fungi: aspergillus spp. blastomyces der ... | 1983 | 6410243 |
| detection of two blastomyces dermatitidis serotypes by exoantigen analysis. | we evaluated the diagnostic value of antibody to blastomyces dermatitidis a precipitin arcs for identifying b. dermatitidis cultures by the exoantigen technique with 88 isolates from the united states and canada, 12 from africa, and 1 each from india and israel. in addition, we studied 190 mycelial-form isolates of other fungi which could be confused antigenically or morphologically with b. dermatitidis. antigen extracts from all of the north american isolates, the indian and israeli isolates, a ... | 1983 | 6411760 |
| blastomyces dermatitidis in india: first report of its isolation from clinical material. | the isolation of blastomyces dermatitidis in india is reported from the bronchial aspirate of a female asthmatic patient who had never travelled abroad. the patient was a resident of rodhain, a small town in the district of badaun (uttar pradesh), situated about 250 km south-east of delhi. apart from its demonstration by culture and direct microscopy of a bronchial aspirate, b. dermatitidis was seen microscopically on two occasions in koh wet mounts and smears of sputum stained with periodic aci ... | 1983 | 6415825 |
| immunohistopathological studies of blastomycosis: the use of labeled specific antigens in a hamster model of disease. | we applied a modified immunofluorescence and immunoperoxidase method, utilizing labeled blastomyces dermatitidis antigens, to look for specific antibody-bearing b/plasma cells in the tissue infiltrates of blastomycosis lesions induced in hamsters. no specific anti-blastomyces antibodies were detectable by this method, although such antibodies were present in blood samples as demonstrated by routine immunodiffusion techniques. these studies suggest that humoral immune reactions do not play a majo ... | 1984 | 6427614 |