| endophyte-induced verticillium protection in tomato is range-restricted. | endophytes, bacterial, fungal or viral, colonize plants often without causing visible symptoms. more important, they may benefit host plants in many ways, most notably by preventing diseases caused by normally virulent pathogens. previous studies have shown that an isolate of v. dahliae from eggplant, dvd-e6, can colonize tomato endophytically, producing taller and more robust tomato plants while providing protection against a virulent v. dahliae, race 1 (vd1) isolate. expression analyses sugges ... | 2009 | 19649201 |
| risk assessment and chemical decontamination of an oxime carbamate insecticide (methomyl) from eggplant, solanum melongena l. | methomyl, s-methyl (ez)-n-(methylcarbamoyloxy)thioacetimidate, is a systemic insecticide chosen for the management of shoot and fruit borer, leucinodes orbonalis g. codex alimentarious commission has proposed a maximum residue limit (mrl) of 0.2 mg kg( - 1) of methomyl, and there is a need to validate this value on eggplant. first + first-order model can explain the nonlinear dissipation pattern of methomyl conveniently in comparison to first-order kinetics. the preharvest intervals (phi) of 27. ... | 2010 | 19757122 |
| roles of the catalytic subunit of camp-dependent protein kinase a in virulence and development of the soilborne plant pathogen verticillium dahliae. | verticillium dahliae is a soilborne fungus that causes vascular wilt disease in a broad range of hosts and survives for many years in the soil in the form of microsclerotia. although the role of camp-dependent protein kinase a (pka) has been extensively studied in foliar pathogens, there is limited information about its role in soilborne fungal pathogens that infect through the root system. genome database search revealed the presence of two pka catalytic subunit genes in v. dahliae, named vdpka ... | 2010 | 20144723 |
| Functional diversification of the GALA type III effector family contributes to Ralstonia solanacearum adaptation on different plant hosts. | Type III effectors from phytopathogenic bacteria exhibit a high degree of functional redundancy, hampering the evaluation of their precise contribution to pathogenicity. This is illustrated by the GALA type III effectors from Ralstonia solanacearum, which have been shown to be collectively, but not individually, required for disease on Arabidopsis thaliana and tomato. We investigated evolution, redundancy and diversification of this family in order to understand the individual contribution of th ... | 2011 | 21902695 |
| genetic variability and evolutionary analyses of the coat protein gene of tomato mosaic virus. | tomato mosaic virus (tomv), a member of the genus tobamovirus, infects several ornamental and horticultural crops worldwide. in this study, the nucleotide sequences of the coat protein gene of worldwide tomv isolates were analyzed to estimate the genetic structure and diversity of this virus and the involved evolutionary forces. the phylogenetic analysis showed three clades with high bootstrap support: clade i contained three tomv isolates from brazil collected from pepper, clade ii comprised on ... | 2011 | 21881940 |
| cardioprotective properties of raw and cooked eggplant (solanum melongena l). | although eggplants are known to be part of a healthy diet, the effects of this fruit on cardioprotection are not known. the present study examined the role of raw and grilled eggplants on cardioprotection using an isolated perfusion heart model. the animals were fed freeze-dried products of either raw or grilled eggplants for 30 days. after 30 days, isolated working hearts were subjected to 30 min ischemia followed by 2 h of reperfusion. left ventricular function was monitored, and myocardial in ... | 2011 | 21894326 |
| eggplant polyphenol oxidase multigene family: cloning, phylogeny, expression analyses and immunolocalization in response to wounding. | though polyphenol oxidase (ppo) genes from tomato and potato have been extensively studied, information about ppo genes in eggplant (solanum melongena) is lacking. the main objective of this study is to understand the structural and functional aspects of eggplant ppo genes. six eggplant ppo genes (smeppo1-6) cloned by race and genome walking were found to be intronless and correspond to eight eggplant unigenes. comprehensive sequence analyses indicated that the eggplant ppo genes exhibit conside ... | 2011 | 21945722 |
| Expression of a cloned sweet potato catalase SPCAT1 alleviates ethephon-mediated leaf senescence and H(2)O(2) elevation. | In this report a full-length cDNA, SPCAT1, was isolated from ethephon-treated mature L3 leaves of sweet potato. SPCAT1 contained 1479 nucleotides (492 amino acids) in its open reading frame, and exhibited high amino acid sequence identities (ca. 71.2-80.9%) with several plant catalases, including Arabidopsis, eggplant, grey mangrove, pea, potato, tobacco and tomato. Gene structural analysis showed that SPCAT1 encoded a catalase and contained a putative conserved internal peroxisomal targeting si ... | 2012 | 21893366 |
| flow injection assessment of nitrate contents in fresh and cooked fruits and vegetables grown in fiji. | nitrates form part of the essential chemistry of soils and plants. thus, plant roots are able to absorb nitrate directly from the soil. it has been discovered that human nitrate intake is mainly from vegetables. vegetables play an important role in human nutrition since they are an outstanding source for vitamins, minerals, and biologically active compounds. in risk assessment of nitrate contents, this study reports the nitrate levels of 8 commonly consumed fruits and vegetables grown in fiji, t ... | 2011 | 22417577 |
| thermal treatment of eggplant (solanum melongena l.) increases the antioxidant content and the inhibitory effect on human neutrophil burst. | the aim of this study was to compare the amount and activity of phytonutrients in raw, grilled, and boiled eggplant fruit using chemical measures and a biological assay of oxidative bursts in human neutrophils. the thermally treated samples showed various changes in their chemical composition (dry matter, soluble solids, acidity, and the amount of alcohol insoluble substances) due to the cooking processes and were much richer in the main phenolic compounds such as chlorogenic and caffeic acids, ... | 2010 | 20187646 |
| [regional differences in gastric cancer mortality and eating habits of people]. | one year (continuous 2 days for 12 months) food surveys were held at 5 areas, with different gastric cancer risks, in akita and iwate prefectures. each food items was counted down the frequency of intake for one year and food index (fi, intake frequency of certain food item or food group/number of meals x 100) was calculated and coefficient of correlations with standardized mortality rates (smrs) of all causes, cancer of all sites and stomach cancer were also calculated. significant negative cor ... | 2013 | 3747143 |
| low-cost phantoms for training of stereotactic vacuum-assisted biopsy of the breast. | to test low-cost phantoms for training in stereotactic breast biopsy, we prepared eggplant with calcium powder; gelatin and turkey breast with coarse salt, peppercorns, and calcium powder, respectively; and short-bread pastry with salt. three to 12 cores were harvested with an 11-gauge vacuum biopsy unit. mammography images were taken before and after biopsy and from the biopsy cores. the pastry phantom provided the best simulation of microcalcifications for stereotactic biopsy with realistic co ... | 2010 | 20189071 |
| wound-induced proteinase inhibitors from tomato leaves. ii. the cdna-deduced primary structure of pre-inhibitor ii. | a cdna containing the complete amino acid-coding region of wound-induced tomato inhibitor ii was constructed in the plasmid puc9. the open reading frame codes for 148 amino acids including a 25-amino acid signal sequence preceding the n-terminal lysine of the mature inhibitor ii. the inhibitor ii sequence exhibits two domains, one domain having a trypsin inhibitory site and the other a chymotrypsin inhibitory site, apparently evolved from a smaller gene by a process of gene duplication and elong ... | 2013 | 3838986 |
| nitrogen hurdle of host alternation for a polyphagous aphid and the associated changes of endosymbionts. | low proportion of essential amino acids (eaas) is one of the barriers for animals to use phloem as a diet. endosymbionts with eaas synthesis functions are considered crucial for ameliorating the lack of eaas in insects' diets. in this study, we transferred the insects from a cabbage-reared myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach. the performance on these plants was evaluated and the dynamics of endosymbionts in relation to this host alternation ... | 2016 | 27094934 |
| combining hexanoic acid plant priming with bacillus thuringiensis insecticidal activity against colorado potato beetle. | interaction between insect herbivores and host plants can be modulated by endogenous and exogenous compounds present in the source of food and might be successfully exploited in colorado potato beetle (cpb) pest management. feeding tests with cpb larvae reared on three solanaceous plants (potato, eggplant and tomato) resulted in variable larval growth rates and differential susceptibility to bacillus thuringiensis cry3aa toxin as a function of the host plant. an inverse correlation with toxicity ... | 2013 | 23743826 |
| gene expression analysis in cadmium-stressed roots of a low cadmium-accumulating solanaceous plant, solanum torvum. | solanum torvum sw. cv. torubamubiga (tb) is a low cadmium (cd)-accumulating plant. to elucidate the molecular mechanisms of the cd acclimation process in tb roots, transcriptional regulation was analysed in response to mild cd treatment: 0.1 mum cdcl(2) in hydroponic solution. a unigene set consisting of 6296 unigene sequences was constructed from 18 816 tb cdnas. the distribution of functional categories was similar to tomato, while 330 unigenes were suggested to be tb specific. for expression ... | 2009 | 19837731 |
| chromosomal evolution in the plant family solanaceae. | over the past decades, extensive comparative mapping research has been performed in the plant family solanaceae. the recent identification of a large set of single-copy conserved orthologous (cosii) markers has greatly accelerated comparative mapping studies among major solanaceous species including tomato, potato, eggplant, pepper and diploid nicotiana species (as well as tetraploid tobacco). the large amount of comparative data now available for these species provides the opportunity to descri ... | 2010 | 20236516 |
| molecular characterization of fusarium oxysporum f. melongenae by issr and rapd markers on eggplant. | fusarium oxysporum f. melongenae is a major soil-borne pathogen of eggplant (solanum melongena). issr and rapd markers were used to characterize fusarium oxysporum f. melongenae isolates collected from eggplant fields in southern turkey. those isolates were not pathogenic to tomato. pathogens were identified by their morphology, and their identity was confirmed by pcr amplification using the specific primer pf02-3. the isolates were classified into groups on the basis of issr and rapd fingerprin ... | 2010 | 20390339 |
| linkage disequilibrium and genome-wide association analysis for anthocyanin pigmentation and fruit color in eggplant. | the genome-wide association (gwa) approach represents an alternative to biparental linkage mapping for determining the genetic basis of trait variation. both approaches rely on recombination to re-arrange the genome, and seek to establish correlations between phenotype and genotype. the major advantages of gwa lie in being able to sample a much wider range of the phenotypic and genotypic variation present, in being able to exploit multiple rounds of historical recombination in many different lin ... | 2014 | 25311640 |
| comparative transcriptome analysis of eggplant (solanum melongena l.) and turkey berry (solanum torvum sw.): phylogenomics and disease resistance analysis. | eggplant (solanum melongena l.) and turkey berry (s. torvum sw.), a wild ally of eggplant with promising multi-disease resistance traits, are of great economic, medicinal and genetic importance, but genomic resources for these species are lacking. in the present study, we sequenced the transcriptomes of eggplant and turkey berry to accelerate research on these two non-model species. | 2014 | 24885385 |
| genome sequencing of ralstonia solanacearum biovar 3, phylotype i, strains rs-09-161 and rs-10-244, isolated from eggplant and chili in india. | ralstonia solanacearum indian strains rs-09-161 and rs-10-244 were isolated from the coastal region of goa and from the andaman islands. we report the draft genome sequences of these representative isolates infecting solanaceous vegetables in india. | 2014 | 24874667 |
| alpha-solasonine and alpha-solamargine contents of gboma (solanum macrocarpon l.) and scarlet (solanum aethiopicum l.) eggplants. | the gboma (solanum macrocarpon l.) and scarlet eggplants (solanum aethiopicum l.), which form part of the traditional sub-saharan african culture, are two of the many neglected crops with potential for increased cultivation or as a genetic resource for improving agronomic traits of the common eggplant. this work is focused on the analysis of glycoalkaloid levels in s. macrocarpon and s. aethiopicum to assess their safety. liquid chromatography-mass spectrometry was used to quantify glycoalkaloid ... | 2010 | 20397650 |
| qtl mapping in eggplant reveals clusters of yield-related loci and orthology with the tomato genome. | in spite of its widespread cultivation and nutritional and economic importance, the eggplant (solanum melongena l.) genome has not been extensively explored. a lack of knowledge of the patterns of inheritance of key agronomic traits has hindered the exploitation of marker technologies to accelerate its genetic improvement. an already established f2 intraspecific population of eggplant bred from the cross '305e40' x '67/3' was phenotyped for 20 agronomically relevant traits at two sites. up to se ... | 2014 | 24586828 |
| vigor for in vitro culture traits in s. melongena × s. aethiopicum hybrids with potential as rootstocks for eggplant. | hybrids of solanum melongena and s. aethiopicum are of interest as rootstocks of eggplant, as they are highly vigorous and can incorporate resistance to several diseases. however, hybridization between both species is difficult. therefore, protocols for in vitro culture are of great interest for their micropropagation and biotechnological breeding. we assessed the organogenesis response from leaf explants in four interspecific hybrids and in their parents testing two organogenic media: sim-a, co ... | 2014 | 24592179 |
| characterization of the endophytic antagonist py11t-3-1 against bacterial soft rot of pinellia ternata. | biocontrol is an emerging trend aimed at reducing chemical input while increasing plant fitness, productivity and resistance to diseases in sustainable agriculture. an antagonist, py11t-3-1, was herein characterized for potential applications against soil-borne plant diseases. | 2010 | 20438622 |
| diffusion and accumulation in cultivated vegetable plants of di-(2-ethylhexyl) phthalate (dehp) from a plastic production factory. | di-(2-ethylhexyl) phthalate (dehp) concentrations in the atmosphere and in four vegetable crops including brassica chinensis l. (bok choy), brassica campestris l. (field mustard), vigna unguiculata walp. (cowpea), and solanum melongena l. (eggplant) cultivated on land surrounding a plastic production factory were determined. the air dehp concentrations (means) at the sites 0.2, 0.4, 0.8, and 1.6 km away from the plastic production building were about 9.4-12.8, 5.8-9.6, 1.6-5.0, and 0.04-0.27 mic ... | 2010 | 20496185 |
| overexpression of the eggplant (solanum melongena) nac family transcription factor smnac suppresses resistance to bacterial wilt. | bacterial wilt (bw) is a serious disease that affects eggplant (solanum melongena) production. although resistance to this disease has been reported, the underlying mechanism is unknown. in this study, we identified a nac family transcription factor (smnac) from eggplant and characterized its expression, its localization at the tissue and subcellular levels, and its role in bw resistance. to this end, transgenic eggplant lines were generated in which the expression of smnac was constitutively up ... | 2016 | 27528282 |
| new insights on eggplant/tomato/pepper synteny and identification of eggplant and pepper orthologous qtl. | eggplant, pepper, and tomato are the most exploited berry-producing vegetables within the solanaceae family. their genomes differ in size, but each has 12 chromosomes which have undergone rearrangements causing a redistribution of loci. the genome sequences of all three species are available but differ in coverage, assembly quality and percentage of anchorage. determining their syntenic relationship and qtl orthology will contribute to exploit genomic resources and genetic data for key agronomic ... | 2016 | 27486463 |
| identification by computer-simulated rflp of phytoplasmas associated with eggplant giant calyx representative of two subgroups, a lineage of 16sriii-j and the new subgroup 16sriii-u. | symptoms resembling giant calyx, a graft-transmissible disease, were observed on 1-5 % of eggplant (aubergine; solanum melongena l.) plants in production fields in sao paulo state, brazil. phytoplasmas were detected in 12 of 12 samples from symptomatic plants that were analysed by a nested pcr assay employing 16s rrna gene primers r16mf2/r16mr1 followed by r16f2n/r16r2. rflp analysis of the resulting rrna gene products (1.2 kb) indicated that all plants contained similar phytoplasmas, each close ... | 2010 | 20622054 |
| draft genome sequences of nine strains of ralstonia solanacearum differing in virulence to eggplant (solanum melongena). | ralstonia solanacearum displays variability in its virulence to solanaceous crops. we report here the draft genome sequences of eight phylotype i strains and one phylotype iii strain differing in virulence to the resistant eggplant genotype ag91-25. these data will allow the identification of virulence- and avirulence-related genes. | 2016 | 26823572 |
| a competitive index assay identifies several ralstonia solanacearum type iii effector mutant strains with reduced fitness in host plants. | ralstonia solanacearum, the causal agent of bacterial wilt, is a soil bacterium which can naturally infect a wide range of host plants through the root system. pathogenicity relies on a type iii secretion system which delivers a large set of approximately 75 type iii effectors (t3e) into plant cells. on several plants, pathogenicity assays based on quantification of wilting symptoms failed to detect a significant contribution of r. solanacearum t3e in this process, thus revealing the collective ... | 2010 | 20687809 |
| the historical role of species from the solanaceae plant family in genetic research. | this article evaluates the main contributions of tomato, tobacco, petunia, potato, pepper and eggplant to classical and molecular plant genetics and genomics since the beginning of the twentieth century. species from the solanaceae family form integral parts of human civilizations as food sources and drugs since thousands of years, and, more recently, as ornamentals. some solanaceous species were subjects of classical and molecular genetic research over the last 100 years. the tomato was one of ... | 2016 | 27744490 |
| crystal structure of the vicilin from solanum melongena reveals existence of different anionic ligands in structurally similar pockets. | crystal structure of a vicilin, sm80.1, was determined towards exploring its possible physiological functions. the protein was purified from solanum melongena by combination of ammonium sulphate fractionation and size exclusion chromatography. structure was determined ab initio at resolution of 1.5 å by x-ray crystallography showing the three-dimensional topology of the trimeric protein. each monomer of sm80.1 consists of two similar domains with hydrophobic binding pocket and each accommodating ... | 2016 | 27004988 |
| antioxidant and hepatoprotective activities of five eggplant varieties. | eggplant is consumed throughout the world and varies in fruit color, shape, and size. in this study, five varieties of eggplant (purple colored moderate size, white-green colored moderate size, long green, green striped moderate size and pale-green colored small size, respectively, called sm1-sm5) were evaluated for total phenolic and flavonoid content, antioxidant activity and hepatoprotection against cytotoxicity of tert-butyl hydroperoxide (t-buooh) in human hepatoma cell lines, hepg2. total ... | 2010 | 20691749 |
| pirna pathway is not required for antiviral defense in drosophila melanogaster. | since its discovery, rna interference has been identified as involved in many different cellular processes, and as a natural antiviral response in plants, nematodes, and insects. in insects, the small interfering rna (sirna) pathway is the major antiviral response. in recent years, the piwi-interacting rna (pirna) pathway also has been implicated in antiviral defense in mosquitoes infected with arboviruses. using drosophila melanogaster and an array of viruses that infect the fruit fly acutely o ... | 2016 | 27357659 |
| pirna pathway gene expression in the malaria vector mosquito anopheles stephensi. | the ability of transposons to mobilize to new places in a genome enables them to introgress rapidly into populations. the pirna pathway has been characterized recently in the germ line of the fruit fly, drosophila melanogaster, and is responsible for downregulating transposon mobility. transposons have been used as tools in mosquitoes to genetically transform a number of species including anopheles stephensi, a vector of human malaria. these mobile genetic elements also have been proposed as too ... | 2014 | 24947897 |
| morphological and molecular characterization of pratylenchus lentis n. sp. (nematoda: pratylenchidae) from sicily. | pratylenchus lentis n. sp. parasitizing roots of lentil in sicily, italy, is described and illustrated. the new species is characterized by a relatively high lip region with three annuli, mean stylet length of 16 mum, with anteriorly flattened knobs, cylindrical body with a relatively anterior vulva, large and ovoid spermatheca full of sperm, plump tail with truncate, irregularly annulated terminus, and by the presence of males. molecular its-rflp and sequencing analyses of the new species showe ... | 0 | 19440258 |
| duplication and diversification of dipteran argonaute genes, and the evolutionary divergence of piwi and aubergine. | genetic studies of drosophila melanogaster have provided a paradigm for rna interference (rnai) in arthropods, in which the microrna and antiviral pathways are each mediated by a single argonaute (ago1 and ago2) and germline suppression of transposable elements is mediated by a trio of piwi-subfamily argonaute proteins (ago3, aub, and piwi). without a suitable evolutionary context, deviations from this can be interpreted as derived or idiosyncratic. here we analyze the evolution of argonaute gen ... | 2016 | 26868596 |
| a role for drosophila cyclin j in oogenesis revealed by genetic interactions with the pirna pathway. | cyclin j (cycj) is a poorly characterized member of the cyclin superfamily of cyclin-dependent kinase regulators, many of which regulate the cell cycle or transcription. although cycj is conserved in metazoans its cellular function has not been identified and no mutant defects have been described. in drosophila, cycj transcript is present primarily in ovaries and very early embryos, suggesting a role in one or both of these tissues. the cycj gene (cycj) lies immediately downstream of armitage (a ... | 2014 | 24946235 |
| de novo assembly and characterization of the transcriptome of grasshopper shirakiacris shirakii. | the grasshopper shirakiacris shirakii is an important agricultural pest and feeds mainly on gramineous plants, thereby causing economic damage to a wide range of crops. however, genomic information on this species is extremely limited thus far, and transcriptome data relevant to insecticide resistance and pest control are also not available. | 2016 | 27455245 |
| molecular insights on the functional microbial community from organic and mineral growing media and its interaction with agrobacterium rhizogenes. | despite numerous preventative measures, the hairy roots syndrome is an increasing problem in greenhouse horticulture. a recent survey of 177 tomato, cucumber and eggplant growers in flanders (belgium) revealed an increase of this disease in the last two years, with about 26% of all the tomato crops showing the syndrome. in this study, we compared the physicochemical and microbial community characteristics of inorganic and organic growing media in relation to the presence of the causative agent o ... | 2014 | 26080470 |
| genetic diversity of eggplant (solanum melongena) germplasm from turkey assessed by ssr and rapd markers. | eggplant is a major crop in turkey, which produces more of this crop than all of europe; consequently, germplasm resources are of concern for the country. molecular characterization of eggplant genotypes collected from different geographical regions of turkey was carried out using ssr and rapd markers. with amplification of five ssr loci, the number of alleles per microsatellite locus ranged from 2 to 10, with a total of 24 alleles. the greatest number of alleles was found at the emf21h22 locus ... | 2010 | 20714999 |
| a role for vasa in regulating mitotic chromosome condensation in drosophila. | vasa (vas) is a conserved dead-box rna helicase expressed in germline cells that localizes to a characteristic perinuclear structure called nuage. previous studies have shown that vas has diverse functions, with roles in regulating mrna translation, germline differentiation, pole plasm assembly, and piwi-interacting rna (pirna)-mediated transposon silencing. although vas has also been implicated in the regulation of germline proliferation in drosophila and mice, little is known about whether vas ... | 2010 | 21185189 |
| ectopic expression of germline genes drives malignant brain tumor growth in drosophila. | model organisms such as the fruit fly drosophila melanogaster can help to elucidate the molecular basis of complex diseases such as cancer. mutations in the drosophila gene lethal (3) malignant brain tumor cause malignant growth in the larval brain. here we show that l(3)mbt tumors exhibited a soma-to-germline transformation through the ectopic expression of genes normally required for germline stemness, fitness, or longevity. orthologs of some of these genes were also expressed in human somatic ... | 2010 | 21205669 |
| tea-industry waste activated carbon, as a novel adsorbent, for separation, preconcentration and speciation of chromium. | activated carbon was produced from tea-industry wastes (tiwac) and employed as a low cost and effective solid phase material for the separation, preconcentration and speciation of chromium species without using a complexing agent, prior to determination by flame atomic absorption spectrometry (faas). the characterization of tiwac was performed by utilizing several techniques such as fourier transform infrared (ftir) spectroscopy, scanning electron microscopy (sem), and elemental analysis. the ad ... | 2010 | 21296208 |
| immunochromatography for the rapid determination of cadmium concentrations in wheat grain and eggplant. | a simple and quick on-site test for trace levels of cadmium (cd) in food is needed because of the human toxicity of this heavy metal. we developed an immunochromatography kit which uses the antigen-antibody complex reaction between the cd-ethylenediaminetetraacetic acid (cd-edta) complex and an anti-cd-edta antibody. we previously reported the successful use of this kit to determine cd concentrations in brown rice with respect to the international standard: 0.4 mg kg?¹. here, we measured, using ... | 2011 | 21337581 |
| non-coding rnas enter mitosis: functions, conservation and implications. | nuage (or commonly known as chromatoid body in mammals) is a conserved germline-specific organelle that has been linked to the piwi-interacting rna (pirna) pathway. pirnas are a class of gonadal-specific rnas that are ~23-29 nucleotides in length and protect genome stability by repressing the expression of deleterious retrotransposons. more recent studies in drosophila have implicated the pirna pathway in other functions including canalization of embryonic development, regulation of maternal gen ... | 2011 | 21356070 |
| transformation of eggplant (solanum melongena l.) using a binary agrobacterium tumefaciens vector. | kanamycin resistant plants of solarium melongena l. (eggplant) cv. picentia were obtained following the cocultivation of leaf explants with agrobacterium tumefaciens. a disarmed binary vector system containing the neomycin phosphotransferase (nptii) gene as the selectable marker and chloramphenicol acetyltransferase (cat) as a reporter gene was utilized. in vitro grown plants were used as sources of explants to produce transgenic plants on selective medium containing 100 mg/l kanamycin. the tran ... | 1990 | 24226372 |
| efficacy of a pyrimidine derivative to control spot disease on solanum melongena caused by alternaria alternata. | the pyrimidine derivative (4,6-dimethyl-n-phenyldiethyl pyrimidine, dpdp) was tested as a foliar spray fungicide at 50 mg l(-1) for protection of eggplant (solanum melongena) from spot disease caused by alternaria alternata. varied concentrations of dpdp (10-50 mg l(-1)) differentially inhibited mycelial growth, conidial count and conidial germination of a. alternata growth in vitro; the magnitude of inhibition increased with increasing concentration. in vivo, an experiment was conducted in pots ... | 2013 | 25685445 |
| involvement of the chloroplastic isoform of trna ligase in the replication of viroids belonging to the family avsunviroidae. | avocado sunblotch viroid, peach latent mosaic viroid, chrysanthemum chlorotic mottle viroid, and eggplant latent viroid (elvd), the four recognized members of the family avsunviroidae, replicate through the symmetric pathway of an rna-to-rna rolling-circle mechanism in chloroplasts of infected cells. viroid oligomeric transcripts of both polarities contain embedded hammerhead ribozymes that, during replication, mediate their self-cleavage to monomeric-length rnas with 5'-hydroxyl and 2',3'-phosp ... | 2012 | 22623792 |
| tomato (solanum lycopersicum): a model fruit-bearing crop. | introductiontomato (solanum lycopersicum) is one of the most important vegetable plants in the world. it originated in western south america, and domestication is thought to have occurred in central america. because of its importance as food, tomato has been bred to improve productivity, fruit quality, and resistance to biotic and abiotic stresses. tomato has been widely used not only as food, but also as research material. the tomato plant has many interesting features such as fleshy fruit, a s ... | 2008 | 21356708 |
| isolation of bacillus amyloliquefaciens s20 and its application in control of eggplant bacterial wilt. | bacterial strain s20 was isolated and identified as bacillus amyloliquefaciens based on physiological and biochemical characteristics and a 16s rrna gene sequence analysis. strain s20 inhibits the growth of fusarium oxysporum and ralstonia solanacearum. some genes associated with the synthesis of some lipopeptides were detected in strain s20 by pcr. iturins a were identified as the main antagonistic substrates by analysis with electrospray ionization mass spectrometry/collision-induced dissociat ... | 2014 | 24632400 |
| developing novel bacterial based bioformulation having pgpr properties for enhanced production of agricultural crops. | plant growth promoting rhizobacteria (pgpr) are beneficial rhizobacteria which enhance plant growth as well as the productivity by a variety of mechanisms pgpr were isolated from the rhizosphere region of som plants (machilus bombycina king) maintained at the central muga eri research and training institute, lahdoigarh, jorhat. a bacterial based bioformulation was prepared and sprayed over the experimental crops including tomato (solanum lycopersicum), cauliflower (brassica oleracea var botrytis ... | 2015 | 25675713 |
| field performance of bt eggplants (solanum melongena l.) in the philippines: cry1ac expression and control of the eggplant fruit and shoot borer (leucinodes orbonalis guenée). | plants expressing cry proteins from the bacterium, bacillus thuringiensis (bt), have become a major tactic for controlling insect pests in maize and cotton globally. however, there are few bt vegetable crops. eggplant (solanum melongena) is a popular vegetable grown throughout asia that is heavily treated with insecticides to control the eggplant fruit and shoot borer, leucinodes orbonalis (efsb). herein we provide the first publicly available data on field performance in asia of eggplant engine ... | 2016 | 27322533 |
| determination of free inositols and other low molecular weight carbohydrates in vegetables. | different low molecular weight carbohydrates including saccharides, polyalcohols, sugar acids, and glycosides have been identified and quantified in different edible vegetables from asteraceae, amarantaceae, amarylidaceae, brassicaceae, dioscoreaceae, and solanaceae families by gas chromatography-mass spectrometry. apart from glucose, fructose, and sucrose, other saccharides such as sedoheptulose in chicory, spinach, cabbage, purple yam, eggplant, radish, and oak leaf lettuce, rutinose in eggpla ... | 2011 | 21366313 |
| histochemical visualization of ros and antioxidant response to viral infections of vegetable crops grown in azerbaijan. | extremes of environmental conditions, such as biotic stresses, strongly affect plant growth and development and may adversely affect photosynthetic process. virus infection is especially problematic in crops, because unlike other diseases, its impact cannot be reduced by phytosanitary treatments. the vegetable crops (solanum lycopеrsicum l, cucurbita melo l., cucumis sativus l., piper longum l., solánum melongéna l., vicia faba l.) showing virus-like symptoms were collected from fields located i ... | 2014 | 24661407 |
| highly specific detection of five exotic quarantine plant viruses using rt-pcr. | to detect five plant viruses (beet black scorch virus, beet necrotic yellow vein virus, eggplant mottled dwarf virus, pelargonium zonate spot virus, and rice yellow mottle virus) for quarantine purposes, we designed 15 rt-pcr primer sets. primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. all but one primer set successfully amplified the targets, and gradient pcrs indicated that the optimal temperature for the 14 useful primer s ... | 2013 | 25288934 |
| effect of tropical rotation crops on meloidogyne incognita and other plant-parasitic nematodes. | in a field experiment conducted on sandy soil in florida during the 1993 season, rotation crops of castor (ricinus communis), velvetbean (mucuna deeringina), 'mississippi silver' cowpea (vigna unguiculata), american jointvetch (aeschynomene americana), 'dehapine 51' cotton (gossypium hirsutum), and 'sx-17' sorghum-sudangrass (sorghum bicolor x s. sudanense) were effective in maintaining low population densities (<12/100 cm(3) soil) of meloidogyne incognita race 1, whereas high population densiti ... | 1995 | 19277319 |
| garlic, from remedy to stimulant: evaluation of antifungal potential reveals diversity in phytoalexin allicin content among garlic cultivars; allicin containing aqueous garlic extracts trigger antioxidants in cucumber. | garlic has the charisma of a potent remedy and holds its repute of a therapeutic panacea since the dawn of civilization. an integrated approach was adopted to evaluate the genetic diversity among chinese garlic cultivars for their antifungal potency as well as allicin content distribution and, furthermore; a bioassay was performed to study the bio-stimulation mechanism of aqueous garlic extracts (age) in the growth and physiology of cucumber (cucumis sativus). initially, 28 garlic cultivars were ... | 2016 | 27610111 |
| origanum dictamnus oil vapour suppresses the development of grey mould in eggplant fruit in vitro. | grey mould rot (botrytis cinerea) development in vitro or in eggplant (solanum melongena l.) fruit was evaluated after treatment with dittany (origanum dictamnus l.) oil (dit) and storage at 12°c and 95% relative humidity during or following exposure to the volatiles. dit volatiles used in different concentration (0-50-100-250 μl/l) and times of exposure (up to 120 h) examined the effects on pathogen development as well as fruit quality parameters. in vitro, fungal colony growth was inhibited wi ... | 2014 | 25254209 |
| solanum melongena: a potential source of antifungal agent. | the antifungal activity of solanum melongena leaf, extracted with petroleum ether, chloroform, methanol and water was evaluated against three human pathogenic dermatophytes namely trichophyton mentagrophytes, t. rubrum and t. tonsurans and two opportunistic fungi candida albicans and trichosporon beigelii. maximum yield of plant components was 4.32 g, extracted in water and minimum 1.07 g in petroleum ether from 150 g of dry plant material. except water extract, all the extracts possessed signif ... | 2010 | 22815574 |
| protein contact dermatitis: review of 27 cases. | background: protein contact dermatitis (pcd) is a rare and underdiagnosed condition that many dermatologists fail to recognize. nevertheless, increasing awareness of the condition and the substances responsible has led to a rise in the number of published cases in recent years. objective: to determine the clinical characteristics and allergens implicated in pcd in our setting. material and methods: a retrospective observational study of all patients diagnosed with pcd in the last 10years was und ... | 2011 | 21497331 |
| restoring pollen fertility in transgenic male-sterile eggplant by cre/loxp-mediated site-specific recombination system. | this study was designed to control plant fertility by cell lethal gene barnase expressing at specific developmental stage and in specific tissue of male organ under the control of cre/loxp system, for heterosis breeding, producing hybrid seed of eggplant. the barnase-coding region was flanked by loxp recognition sites for cre-recombinase. the eggplant inbred/pure line ('e-38') was transformed with cre gene and the inbred/pure line ('e-8') was transformed with the barnase gene situated between lo ... | 2010 | 21637486 |
| radioactivity and heavy metal concentrations in food samples from rize, turkey. | background: rize in turkey was contaminated by the chernobyl accident in 1986. a comprehensive study was planned and carried out to determine the radioactivity levels and heavy metal concentrations in four food categories collected in rize in 2008, 2009 and 2010. results: tomato showed the highest concentration of (238) u, at 9.43 ± 0.128 bq kg(-1) , whereas the lowest concentration of 0.20 ± 0.02 bq kg(-1) was measured in aubergine samples. the highest concentration of (232) th was measured at ... | 2011 | 21815162 |
| [evaluation of a new medium, eggplant (solanum melongena) agar as a screening medium for cryptococcus neoformans in environmental samples]. | cryptococcus neofomans is an encapsulated yeast-like fungus that causes life-threatening infections, especially in immunosuppresive patients. c.neoformans infection is believed to be acquired via inhalation of aerosolized particles from the environment. avian guano, decaying tree hollows and soil are the related known environmental niches. brown pigmented yeast growth from the precursors in growth media is an important step for the identification and isolation of c.neoformans. seeds of plants in ... | 2014 | 24819266 |
| virus surveys of capsicum spp. in the republic of benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species. | surveys were conducted in 2014 and 2015 in southern and northern benin, respectively, to identify the viruses infecting peppers (capsicum spp.). the samples were screened by elisa for cucumber mosaic virus (cmv), pepper veinal mottle virus (pvmv), potato virus y (pvy) and tomato yellow leaf curl virus (tylcv). a generic reverse transcription pcr (rt-pcr) was used to test for the presence of poleroviruses. elisa tests confirmed the prevalence of all viruses, while the rt-pcr detected pepper vein ... | 2017 | 28220324 |
| molecular identification of cucumber mosaic virus isolates of subgroup ib associated with mosaic disease of eggplant in india. | association of cucumber mosaic virus (cmv) with severe mosaic disease of eggplant (solanum melongena l.) collected from lucknow and kanpur, india was initially detected by host reaction and serological assay and confirmed by rt-pcr employing coat protein gene specific primers. further, molecular identification of the virus isolates was done by cloning and sequence analysis of the complete rna3 genome. based on 97-99 % identities and phylogenetic relationships, the virus isolates infecting eggpla ... | 2014 | 24426321 |
| first report of cucumber mosaic virus subgroups i and ii on soybean, pea, and eggplant in iran. | cucumber mosaic virus (cmv) has the widest host range (> 1000 plant species) of any known plant virus, a large number of vector species, and a wide geographical distribution. a survey was conducted to determine the incidence of cmv of subgroups i and ii on selected host crops in northern iran. a total of 935 leaf samples from 10 host crops (tomato, pea, tobacco, soybean, watermelon, broad bean, squash, cucumber, eggplant, and lettuce) showing virus disease-like symptoms were collected in 12 citi ... | 2012 | 22720706 |
| two new antioxidant malonated caffeoylquinic acid isomers in fruits of wild eggplant relatives. | fruits of the cultivated eggplant species <i>solanum melongena</i> and its wild relative <i>solanum incanum</i> have a high content of hydroxycinnamic acid conjugates, which are implicated in the human health benefits of various fruits and vegetables. monocaffeoylquinic acid esters, in particular 5-<i>o</i>-(-<i>e</i>)-caffeoylquinic acid, are usually predominant in solanaceous fruits and tubers. two closely related caffeoylquinic acid derivatives with longer c<sub>18</sub>-hplc retention times ... | 2011 | 21800866 |
| a plant virus manipulates the behavior of its whitefly vector to enhance its transmission efficiency and spread. | plant viruses can produce direct and plant-mediated indirect effects on their insect vectors, modifying their life cycle, fitness and behavior. viruses may benefit from such changes leading to enhanced transmission efficiency and spread. in our study, female adults of bemisia tabaci were subjected to an acquisition access period of 72 h in tomato yellow leaf curl virus (tylcv)-infected and non-infected tomato plants to obtain viruliferous and non-viruliferous whiteflies, respectively. insects th ... | 2013 | 23613872 |
| eggplant latent viroid: a friendly experimental system in the family avsunviroidae. | eggplant latent viroid (elvd) is the only species of the genus elaviroid (family avsunviroidae). all the viroids in the family avsunviroidae contain hammerhead ribozymes in the strands of both polarities, and are considered to replicate in the chloroplasts of infected cells. this family includes two other genera: avsunviroid and pelamoviroid. | 2016 | 26696449 |
| the transcription initiation sites of eggplant latent viroid strands map within distinct motifs in their in vivo rna conformations. | eggplant latent viroid (elvd), like other members of family avsunviroidae, replicates in plastids through a symmetric rolling-circle mechanism in which elongation of rna strands is most likely catalyzed by a nuclear-encoded polymerase (nep) translocated to plastids. here we have addressed where nep initiates transcription of viroid strands. because this step is presumably directed by sequence/structural motifs, we have previously determined the conformation of the monomeric linear (+) and (-) rn ... | 2016 | 26618399 |
| introgression of bacterial wilt resistance from eggplant to potato via protoplast fusion and genome components of the hybrids. | bacterial wilt resistant somatic hybrids were obtained via protoplast fusion between potato and eggplant and three types of nuclear genomes were identified in the hybrids through gish and ssr analysis. cultivated potato (solanum tuberosum l.) lacks resistance to bacterial wilt caused by ralstonia solanacearum. interspecific symmetric protoplast fusion was conducted to transfer bacterial wilt resistance from eggplant (s. melongena, 2n = 2x = 24) into dihaploid potato (2n = 2x = 24). in total, 34 ... | 2013 | 23912850 |
| [influence of food browning on nutrition. iii. effect of brown substances on digestive enzyme activity (author's transl)]. | in the present study, we separated and partially purified brown substances from eggplants and examined their inhibitory action on trypsin activity. the following results were obtained: 1. the first half of the elute after passing ethanol-extractable brown substances through deae-cellulose column showed no inhibitory action on trypsin, whereas the middle portion of the elute contained a trypsin inhibitor(s). similar results were obtained after fractionation with sephadex g-25. the degree of inhib ... | 2005 | 544414 |
| genomic analysis of the native european solanum species, s. dulcamara. | solanum dulcamara (bittersweet, climbing nightshade) is one of the few species of the solanaceae family native to europe. as a common weed it is adapted to a wide range of ecological niches and it has long been recognized as one of the alternative hosts for pathogens and pests responsible for many important diseases in potato, such as phytophthora. at the same time, it may represent an alternative source of resistance genes against these diseases. despite its unique ecology and potential as a ge ... | 2013 | 23713999 |
| significant effects due to peptone in kelman medium on colony characteristics and virulence of ralstonia solanacearum in tomato. | the study was taken up to assess if the media constituents played any role in governing the variable colony characteristics or pathogenicity of the bacterial wilt pathogen, ralstonia solanacearum cultured on the widely employed kelman medium. the effects due to the constituents 2,3,5-triphenyl tetrazolium chloride (ttc), peptone, casein hydrolysate and glucose on colony characteristics were investigated using -80°c stored culture of strain 'nh-av01' (race 1, biovar 3) isolated from tomato. compa ... | 2014 | 25408775 |
| diversity, biocontrol, and plant growth promoting abilities of xylem residing bacteria from solanaceous crops. | eggplant (solanum melongena l.) is one of the solanaceous crops of economic and cultural importance and is widely cultivated in the state of goa, india. eggplant cultivation is severely affected by bacterial wilt caused by ralstonia solanacearum that colonizes the xylem tissue. in this study, 167 bacteria were isolated from the xylem of healthy eggplant, chilli, and solanum torvum sw. by vacuum infiltration and maceration. amplified rdna restriction analysis (ardra) grouped these xylem residing ... | 2014 | 24963298 |
| genetic variation of eggplant mottled dwarf virus from annual and perennial plant hosts. | the genetic diversity of eggplant mottled dwarf virus (emdv), a member of the family rhabdoviridae, was studied using isolates collected from different herbaceous and woody plant species and remote geographic areas. sequences corresponding to the n, x, p, y, m and g orfs as well as the untranslated regions (utrs) between orfs were determined from all isolates. low genetic diversity was found in almost all genomic regions studied except for the x orf and the utrs, which were more variable, while ... | 2016 | 26660163 |
| development of one-tube real-time qrt-pcr and evaluation of rna extraction methods for the detection of eggplant mottled dwarf virus in different species. | a one-tube real-time qrt-pcr assay was developed, for the detection and quantification of eggplant mottled dwarf virus (emdv), a pathogen affecting cultivated and ornamental plants. the amplification efficiency of the assay was 98% and the linear range of quantification was from 20 to 2×10(8) rna transcripts. total rna extraction methods (three developed methods and one commercially available rna extraction kit) were evaluated using tissues from seven different plant species and synthetic emdv r ... | 2015 | 25445799 |
| a novel strategy for the determination of a rhabdovirus genome and its application to sequencing of eggplant mottled dwarf virus. | a novel strategy employing the rhabdovirus untranslated conserved intergenic regions was developed and applied successfully for the determination of the complete nucleotide sequence of eggplant mottled dwarf virus (emdv). the emdv genome contains seven open reading frames with the same organization as potato yellow dwarf virus (pydv), the type species of the genus nucleorhabdovirus. these two species encode five core genes [nucleocapsid (n), phosphoprotein (p), matrix (m), glycoprotein (g), and ... | 2013 | 23558438 |
| lectins as probes for the assay of rhabdovirus infections in plants. | thirteen different, biotinylated plant lectins were tested for their ability to recognize specifically the glycoproteins of the two different plant rhabdoviruses potato yellow dwarf virus and eggplant mottled dwarf virus. all viruses were propagated on the same plant host species, nicotiana rustica l. the lectin-binding to the viral proteins was tested after electrophoretic separation and transfer to nitrocellulose membranes. besides purified virus also partially pure virus preparations were use ... | 1987 | 3680462 |
| a study of the structure of eggplant mottled dwarf virus. | | 1973 | 4139808 |
| prevalence of entomophthoralean fungi (entomophthoromycota) of aphids (hemiptera: aphididae) on solanaceous crops in argentina. | solanum melongena l. and capsicum annuum l. were sampled in argentina to determine the prevalence of fungal diseased aphids. the pathogens identified were pandora neoaphidis (remaudière & hennebert) humber and zoophthora radicans (brefeld) batko (entomophthorales: entomophthoraceae) on aphids from eggplants; and p. neoaphidis and entomophthora planchoniana cornu (entomophthorales: entomophthoraceae) on aphids from peppers. the highest fungal prevalence was 45.5% (n=2296) and 98.1% (n=3212) from ... | 2014 | 24956128 |
| the wrky transcription factor genes in eggplant (solanum melongena l.) and turkey berry (solanum torvum sw.). | wrky transcription factors, which play critical roles in stress responses, have not been characterized in eggplant or its wild relative, turkey berry. the recent availability of rna-sequencing data provides the opportunity to examine wrky genes from a global perspective. we identified 50 and 62 wrky genes in eggplant (smelwrkys) and turkey berry (storwrkys), respectively, all of which could be classified into three groups (i-iii) based on the wrky protein structure. the smelwrkys and storwrkys c ... | 2015 | 25853261 |
| resequencing of capsicum annuum parental lines (ycm334 and taean) for the genetic analysis of bacterial wilt resistance. | bacterial wilt (bw) is a widespread plant disease that affects a broad range of dicot and monocot hosts and is particularly harmful for solanaceous plants, such as pepper, tomato, and eggplant. the pathogen responsible for bw is the soil-borne bacterium, ralstonia solanacearum, which can adapt to diverse temperature conditions and is found in climates ranging from tropical to temperate. resistance to bw has been detected in some pepper plant lines; however, the genomic loci and alleles that medi ... | 2016 | 27793102 |
| draft genome sequence of an isolate of fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt of eggplant. | here, we present the genome sequence of an isolate (14004) of fusarium oxysporum f. sp. melongenae, an eggplant pathogen. the final assembly consists of 1,631 scaffolds with 53,986,354 bp (g+c content, 46.4%) and 16,485 predicted genes. | 2017 | 28209821 |
| transcriptome analysis and molecular marker discovery in solanum incanum and s. aethiopicum, two close relatives of the common eggplant (solanum melongena) with interest for breeding. | solanum incanum is a close wild relative of s. melongena with high contents of bioactive phenolics and drought tolerance. s. aethiopicum is a cultivated african eggplant cross-compatible with s. melongena. despite their great interest in s. melongena breeding programs, the genomic resources for these species are scarce. | 2016 | 27108408 |
| detailed mapping of a resistance locus against fusarium wilt in cultivated eggplant (solanum melongena). | this is the first report on genetic mapping of a resistance locus against fusarium wilt caused by the plant pathogen fusarium oxysporum f. sp. melongenae in cultivated eggplant. | 2016 | 26582508 |
| identification of mirna from eggplant (solanum melongena l.) by small rna deep sequencing and their response to verticillium dahliae infection. | mirnas are a class of non-coding small rnas that play important roles in the regulation of gene expression. although plant mirnas have been extensively studied in model systems, less is known in other plants with limited genome sequence data, including eggplant (solanum melongena l.). to identify mirnas in eggplant and their response to verticillium dahliae infection, a fungal pathogen for which clear understanding of infection mechanisms and effective cure methods are currently lacking, we deep ... | 2013 | 24015279 |
| zucchini and squash encode two putative nucleases required for rasirna production in the drosophila germline. | rnai is a widespread mechanism by which organisms regulate gene expression and defend their genomes against viruses and transposable elements. here we report the identification of drosophila zucchini (zuc) and squash (squ), which function in germline rnai processes. zuc and squ contain domains with homologies to nucleases. mutant females are sterile and show dorsoventral patterning defects during oogenesis. in addition, oskar protein is ectopically expressed in early oocytes, where it is normall ... | 2007 | 17543859 |
| overexpression of the ascorbate peroxidase gene from eggplant and sponge gourd enhances flood tolerance in transgenic arabidopsis. | previously, we found that the flood resistance of eggplant (solanum melongena) and sponge gourd (luffa cylindrica) enhanced ascorbate peroxidase (apx) activity under flooding, and consequently, both the smapx and lcapx genes were cloned. in this study, the smapx and lcapx genes were transferred under a ubiquitin promoter to arabidopsis (at) via agrobacterium tumefaciens. the expression and amount of apx and apx activities of the smapx and lcapx transgenic lines were significantly higher than tho ... | 2017 | 28150172 |
| a description of the lactic acid bacteria microbiota associated with the production of traditional fermented vegetables in vietnam. | an important part of the daily nourishment in vietnam constitutes of fermented vegetables. bacteria and especially lactic acid bacteria play a central role in the production of many fermented vegetables. the current study was conducted to investigate the diversity of native lactic acid bacteria (lab) populations in 'dua muoi' (mustard and beet fermentation) and 'ca muoi' (eggplant fermentation), three types of popular traditional fermented vegetables of vietnamese origin. to this end a polyphasi ... | 2013 | 23500611 |
| generation of selectable marker-free transgenic eggplant resistant to alternaria solani using the r/rs site-specific recombination system. | marker-free transgenic eggplants, exhibiting enhanced resistance to alternaria solani , can be generated on plant growth regulators (pgrs)- and antibiotic-free ms medium employing the multi-auto-transformation (mat) vector, pmat21 - wasabi defensin , wherein isopentenyl transferase ( ipt ) gene is used as a positive selection marker. | 2014 | 24311155 |
| tropical rotation crops influence nematode densities and vegetable yields. | the effects of eight summer rotation crops on nematode densities and yields of subsequent spring vegetable crops were determined in field studies conducted in north florida from 1991 to 1993. the crop sequence was as follows: (i) rotation crops during summer 1991; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) 'lemondrop l' squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) 'classic' eggplant (solanum melo ... | 1994 | 19279897 |
| effects of tropical rotation crops on meloidogyne arenaria population densities and vegetable yields in microplots. | the effects of 12 summer crop rotation treatments on population densities of meloidogyne arenaria race 1 and on yields of subsequent spring vegetable crops were determined in microplots. the crop sequence was: (i) rotation crops during summer 1991 ; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) eggplant (solanum melongena) during spring 1993. the 12 rotat ... | 1994 | 19279880 |
| assessment of factors influencing the agrobacterium-mediated in planta seed transformation of brinjal (solanum melongena l.). | an efficient and reproducible in planta transformation method was developed for brinjal using seed as an explant. the brinjal seeds were infected with agrobacterium tumefaciens eha 105 harbouring pcambia 1301-bar plasmid, and the transformants were selected against basta®. several parameters influencing the in planta seed transformation such as pre-culture duration, acetosyringone concentration, surfactants, duration of sonication, vacuum pressure and vacuum duration have been evaluated. the put ... | 2013 | 23852797 |
| efficacy of various application methods of fluensulfone for managing root-knot nematodes in vegetables. | fluensulfone is a new nematicide in the flouroalkenyl chemical group. a field experiment was conducted in 2012 and 2013 to evaluate the efficacy of various application methods of fluensulfone for control of meloidogyne spp. in cucumber (cucumis sativus). treatments of fluensulfone (3.0 kg a.i./ha) were applied either as preplant incorporation (ppi) or via different drip irrigation methods: drip without pulse irrigation (drip np), pulse irrigation 1 hr after treatment (drip +1p), and treatment at ... | 2016 | 27418698 |
| asymmetric somatic hybrid plants between an interspecific lycopersicon hybrid and solanum melongena. | asymmetric somatic hybrid plants were obtained by a modified peg/dmso fusion procedure between protoplasts derived from suspension cells of an interspecific tomato hybrid, lycopersicon esculentum x l. pennellii, and mesophyll protoplasts of solanum melongena, eggplant. the tomato hybrid was previously transformed with agrobacterium tumefaciens and contained the kanamycin-resistance marker gene. prior to fusion, the donor protoplasts of the tomato hybrid were gamma irradiated at 9.0 krad. thus, n ... | 1995 | 24194315 |
| transformation of solanum integrifolium poir via agrobacterium tumefaciens: plant regeneration and progeny analysis. | the wild species solanum integrifolium represents a source of pest and disease resistance genes for breeding strategies of the cultivated species solanum melongena. somatic hybridization via protoplast fusion between the two species may provide a valuable tool for transferring polygenic traits into the cultivated species. the availability of s.integrifolium cells carrying dominant selectable markers would facilitate the heterokaryon rescue. an appropriate methodology for in vitro culture and pla ... | 1992 | 24213029 |
| somatic hybridization between selected lycopersicon and solanum species. | mesophyll protoplasts of an interspecific lycopersicon esculentum mill, (tomato) x lycopersicon pennellii hybrid plant (ep) were fused with callus-derived protoplasts of solanum lycopersicoides dun. using a modified peg/dmso procedure. the ep plant was previously transformed by agrobacterium tumefaciens which carried the nptii and nopaline synthase genes. protoplasts were plated at 10(5)/ml in modified km medium and 16 days post-fusion 25 ug/ml kanamycin was added to the culture medium. during s ... | 1991 | 24221398 |