population genetic structure of japanese wild soybean (glycine soja) based on microsatellite variation. | the research objectives were to determine aspects of the population dynamics relevant to effective monitoring of gene flow in the soybean crop complex in japan. using 20 microsatellite primers, 616 individuals from 77 wild soybean (glycine soja) populations were analysed. all samples were of small seed size (< 0.03 g), were directly collected in the field and came from all parts of japan where wild soybeans grow, except hokkaido. japanese wild soybean showed significant reduction in observed het ... | 2006 | 16599960 |
generation and analysis of expressed sequence tags from nacl-treated glycine soja. | salinization causes negative effects on plant productivity and poses an increasingly serious threat to the sustainability of agriculture. wild soybean (glycine soja) can survive in highly saline conditions, therefore provides an ideal candidate plant system for salt tolerance gene mining. | 2006 | 16504061 |
entity evidence for differentiation between tia and tib types of soybean kunitz trypsin inhibitor: detection of a novel transitional variant type between tia and tib in wild soybean (glycine soja sieb. & zucc.). | soybean kunit trypsin inhibitor (skti) has several polymorphic types. of these skti, there are large differences of nine amino acid substitutions between tia and tib. so far no transitional type between them has been found. a novel transitional intermediate variant between tia and tib was detected in 11 lines from 720 japanese wild soybeans (glycine soja sieb. & zucc.). this variant showed identical electrophoretic mobility to tib in the davis system polyacrylamide gel electrophoresis (page), bu ... | 2005 | 16228192 |
differential sensitivity to chloride and sodium ions in seedlings of glycine max and g. soja under nacl stress. | high na+ and cl- concentrations in soil cause hyperionic and hyperosmotic stress effects, the consequence of which can be plant demise. ion-specific stress effects of na+ and cl- on seedlings of cultivated (glycine max (l.) merr) and wild soybean (glycine soja sieb. et zucc.) were evaluated and compared in isoosmotic solutions of cl-, na+ and nacl. results showed that under nacl stress, cl- was more toxic than na+ to seedlings of g. max. injury of six g. max cultivars, including 'jackson' (salt ... | 2005 | 16173461 |
genetically modified and wild soybeans: an immunologic comparison. | most traits introduced into genetically engineered crops result from the expression of new proteins. as the first step toward assessing the allergenic potential of genetically modified organism (gmo) food, immunologic and physicochemical characterizations are needed. we prepared crude extract from gmo soybeans, wild soybeans, curd, and soy milk and then performed sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). after acidification with hcl, the samples were separated to glob ... | 2016 | 16119037 |
molecular analysis and physicochemical properties of electrophoretic variants of wild soybean glycine soja storage proteins. | cultivated soybeans (glycine max) are derived from wild soybeans (glycine soja) and can be crossed with them to produce fertile offspring. the latter exhibit greater genetic variation than the former, suggesting a possibility that wild soybeans contain storage proteins with properties different from and better than those of cultivated soybeans. to identify a wild soybean suitable for breeding a new soybean cultivar, we analyzed seed proteins from 390 lines of wild soybeans by electrophoresis. we ... | 2005 | 15853416 |
[changes of polyamines level in glycine soja and glycine max seedlings under nacl stress]. | with internationally common-used glycine max (the salt-tolerant lee68) and glycine soja (the salt-sensitive n23232) as reference, this paper studied the polyamines (pas) contents and polyamine oxidase (pao) activities in the highly salt-tolerant bb52 (glycine soja) seedlings, which showed that under 150mmol x l(-1) nacl stress for 2d, the decrease of put and spd contents was more significant, but that of spd content was less significant in roots of bb52 than in those of lee68 and n23232. for lea ... | 2004 | 15506103 |
multigene family for bowman-birk type proteinase inhibitors of wild soja and soybean: the presence of two bbi-a genes and pseudogenes. | genes for bowman-birk type protease inhibitors (bbis) of wild soja (glycine soja) and soybean (glycine max) comprise a multigene family. the organization of the genes for wild soja bbis (wbbis) was elucidated by an analysis of their cdnas and the corresponding genomic sequences, and compared with the counterparts in the soybean. the cdnas encoding three types of wild soja bbis (wbbi-a, -c, and -d) were cloned. two subtypes of cdnas for wbbi-a, designated wbbi-a1 and -a2, were further identified. ... | 2004 | 15215592 |
[salt tolerance of wild soybeans in jiangsu and its relation with ionic distribution and selective transportation]. | the salt tolerance of 3 wild soybean (glycine soja) populations was compared with their relative germination and seedling emergence. the results showed that the salt tolerance of jiangsu wild soybean (jws) was the strongest. under nacl stress, the dry matter accumulation in wild soybean seedlings decreased, root/shoot ratio increased, and these effects were greater in n23232 (salt-sensitive) than in jws (salt-tolerant). determinations on the ion contents in different organs indicated that the na ... | 2003 | 15031924 |
gmpap3, a novel purple acid phosphatase-like gene in soybean induced by nacl stress but not phosphorus deficiency. | purple acid phosphatases (paps) are commonly found in plants, but the physiological functions of different classes of paps are not thoroughly understood. in the present study, we identified a novel gene, gmpap3, from salt-stressed soybean using suppression subtractive hybridization (ssh) techniques. protein sequence alignment studies and phylogenetic analysis strongly suggested that gmpap3 belongs to the group of plant paps and pap-like proteins that are distinct from those of fungi and animals. ... | 2003 | 14585503 |
identification of putative qtl that underlie yield in interspecific soybean backcross populations. | glycine soja, the wild progenitor of soybean, is a potential source of useful genetic variation in soybean improvement. the objective of our study was to map quantitative trait loci (qtl) from g. soja that could improve the crop. five populations of bc(2)f(4)-derived lines were developed using the glycine max cultivar ia2008 as a recurrent parent and the g. soja plant introduction (pi) 468916 as a donor parent. there were between 57 and 112 bc(2)f(4)-derived lines in each population and a total ... | 2004 | 14504749 |
assignment of molecular linkage groups to soybean chromosomes by primary trisomics. | gene-linkage groups (classical linkage groups, clgs; molecular linkage groups, mlgs) and chromosome relationship in soybean [ glycine max (l.) merr., 2n = 40] is not yet established. however, primary trisomics provide an invaluable cytogenetic tool to associate genes and linkage groups to specific chromosomes. we have assigned 11 mlgs to soybean chromosomes by using primary trisomics (2 x + 1 = 41) and ssr markers. primary trisomics were hybridized with glycine soja sieb. and zucc. (2n = 40) in ... | 2003 | 12783169 |
sire1, an endogenous retrovirus family from glycine max, is highly homogeneous and evolutionarily young. | sire1 is unusual among ty1-copia retrotransposons in that it has an additional open reading frame with structural features similar to retroviral envelope proteins between pol and the 3' ltr. here we report the characterization and comparison of eight different sire1 elements derived from a soybean genomic library, as well as sire1 reverse transcriptases from glycine soja. the dna sequences of the eight sire1 elements are highly homogeneous and share greater than 95% nucleotide identity. partial ... | 2003 | 12777503 |
introgression of a quantitative trait locus for yield from glycine soja into commercial soybean cultivars. | the value of exotic germplasm in broadening the genetic base of most crops has been demonstrated many times. however, the difficulties involved in working with exotic germplasm have limited their utility in plant breeding. unwanted linkages often thwart the successful incorporation of beneficial exotic genes into commercial lines. thus, the use of exotics in traditional breeding makes the process of crop improvement a tedious, time-consuming and expensive endeavor. the availability of molecular ... | 2003 | 12595984 |
diversity of chloroplast dna ssrs in wild and cultivated soybeans: evidence for multiple origins of cultivated soybean. | soybean [ glycine max (l.) merr.] is one of the major crops in the world and was domesticated from a wild progenitor, glycine soja sieb. & zucc., in east asia. in order to address the questions concerning the evolution and maternal lineage of soybean, we surveyed the variation in chloroplast dna simple sequence repeats (cpssr) of 326 wild and cultivated soybean accessions that were collected from various asian countries. twenty-three variants were detected at six cpssrs in the accessions tested. ... | 2002 | 12582476 |
[genetically determined enzyme polymorphism in soy varieties (glycine max) and in wild soy (glycine soja)]. | analysis of 22 genetic-biochemical systems (42 loci) in 18 varieties of domestic soybean (g. max) and in 3 population of wild soybean (g. soja) was carried out. the part of polymorphous loci (p), intraspecies genetic differentiation (genetic distances--dn) were higher in domestic plants in comparison with wild ones (p = 45%, 17%: dn = 0.038-0.269, 0.059-0129). the preferable polymorphism of loci, coding the enzymes of glycolysis and kreb's cycle was revealed in wild species. domestic soybean had ... | 2008 | 10857206 |
phylogenetic relationships of the mitochondrial genomes in the genus glycine subgenus soja. | restriction fragment length polymorphisms (rflps) of mitochondrial dna (mtdna) of wild and cultivated soybeans were analyzed to study their phylogenetic relationships. the observed number of differences in hybridization profiles greatly varied (1-17 patterns) with both the mtdna probes and the restriction enzymes that were used. a cladistic analysis was conducted based on the rflp data. in the parsimonious tree, four distinct groups appeared among 20 accessions of the subgenus soja representing ... | 1998 | 9880923 |
the nuclear ribosomal dna intergenic spacers of wild and cultivated soybean have low variation and cryptic subrepeats. | the intergenic ribosomal dna spacers (igss) from cultivated soybean (glycine max) and wild soybean (glycine soja) were sequenced and compared with six other legumes. these igs sequences were 1821 bp in length in g. soja and g. max cultivars arksoy, ransom, and tokyo, and 1823 bp long in the g. max cultivar columbus. these represent the smallest published plant igs sequences to data. two clones from each of the above five cultivars were sequenced and only 22 sites (1.2%) were polymorphic, thereby ... | 1998 | 9644827 |
ultradian movements of shoots of two species of soybeans glycine soja (sieb. and zucc.) and glycine max (l.) merr. | circumnutation movements of shoot tips of two species of soybean were studied. shoot tips of glycine soja (sieb. and zucc.) moved in a counterclockwise direction with a mean period (+/- sd) of 108 +/- 9 minutes when the shoots were not handled prior to monitoring (n = 17). effects of mechanical stress or stimulation (thigmomorphogenesis) resulted in the lengthening of the mean period by 15% when shoot lengths were measured (handled) prior to monitoring movements (124 +/- 18 minutes, n = 49) or h ... | 1998 | 9493709 |
phylogenetic and genomic relationships in the genus glycine willd. based on sequences from the its region of nuclear rdna. | phylogenetic relationships among all 18 species of the genus glycine were inferred from nucleotide sequence variation in the internal transcribed spacer (its) region of nuclear ribosomal dna. pairwise sequence divergence values ranged from 0.2% (a single nucleotide) between glycine max and glycine soja to 8.6% between glycine hirticaulis and glycine falcata. the length of the its1 and its2 sequences ranged from 215 to 238 nucleotides and from 205 to 222 nucleotides, respectively, and that of 5.8 ... | 1997 | 9061914 |
use of field observations to characterise genotypic flowering responses to photoperiod and temperature: a soyabean exemplar. | thirty-nine accessions of soyabean [glycine max (l.) merrill] and 1 of wild annual soyabean (glycine soja l.) were sown at two sites in taiwan in 1989 and 1990 and on six occasions during 1990 at one site in queensland, australia. on two of the occasions in australia additional treatments extended natural daylengths by 0.5 h and 2 h. the number of days from sowing for the first flower to appear on 50% of the plants in each treatment was recorded (f), and from these values the rate of progress to ... | 1996 | 24162343 |
5s rrna genes in tribe phaseoleae: array size, number, and dynamics. | the organization of 5s rrna genes in plants belonging to tribe phaseoleae was investigated by clamped homogeneous electric field gel electrophoresis and southern blot hybridization. representatives of subtribe glycininae included the diploid species neonotonia wightii and teramnus labialis, as well as three soybean accessions: an elite glycine max (l.) merr. cultivar (bsr101), an unadapted g. max introduction (pi 437.654), and a wild glycine soja (pi 468.916). a cultivar of phaseolus vulgaris (k ... | 1996 | 18469906 |
a major satellite dna of soybean is a 92-base pairs tandem repeat. | we report the cloning, sequencing and analysis of the major repetitive dna of soybean (glycine max). the repeat, sb92, was cloned as several monomers and trimers produced by digestion with xhoi. the deduced consensus sequence of the repeat is 92 base pairs long. genomic sequences do not fluctuate in length. their average homology to the consensus sequence is 92%. the consensus of sb92 contains slightly degenerated homologies for several 6-cutters. therefore, many of them generate a ladder of 92- ... | 1995 | 24174019 |
genetic mapping and variability of seven soybean simple sequence repeat loci. | microsatellites or simple sequence repeats are stretches of short tandemly repeated dna sequence motifs, dispersed throughout the genomes of most eukaryotes. simple sequence repeat polymorphisms (ssrps) have recently been reported in plants. here we present the genetic map position of seven different soybean (glycine max (l.) merr. and glycine soja sieb. and zucc.) ssrps contained in sequenced genes, four of which represent newly mapped positions for these genes. the other three ssrps coincided ... | 1994 | 8001811 |
inheritance of polymorphic markers generated by dna amplification fingerprinting and their use as genetic markers in soybean. | dna amplification fingerprinting (daf) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (aflp) in soybean (glycine max (l.) merr.). the inheritance of aflps was studied using a cross between the ancestral glycine soja pi468.397 and glycine max (l.) merr. line nts382, f1 and f2 progeny. the amplification reaction was carried out with soybean genomic dna and 8 base long oligonucleotide primers. silver ... | 1994 | 7948860 |
genetic and chemical polymorphisms of saponins in soybean seed. | the variation in saponin composition in soybean seeds is explained by different combinations of five genes controlling the utilization of soyasapogenol glycosides as substrates. the function of these genes is variety-specific and organ-specific. phenotypes of over 1000 soybeans were classified into eight saponin types, and the frequency of phenotypes was different between the cultivated [glycine max (l.) merr.] and the wild soybean (g. soja sieb. & zucc.). the aabc saponin type predominated in g ... | 1993 | 7764284 |
rflp mapping in soybean: association between marker loci and variation in quantitative traits. | we have constructed a genetic map for soybean and identified associations between genetic markers and quantitative trait loci. one-hundred-fifty restriction fragment length polymorphisms (rflps) were used to identify genetic linkages in an f2 segregating population from an interspecific cross (glycine max x glycine soja). twenty-six genetic linkage groups containing ca. 1200 recombination units are reported. progeny-testing of f2-derived families allowed quantitative traits to be evaluated in re ... | 1990 | 1979039 |
genetic analysis of soybean hard seededness with molecular markers. | hard seededness in soybean [glycine max (l.) merr.] is a quantitative trait that affects the germination rate, viability, and quality of stored seeds. we have used 72 restriction fragment length polymorphisms (rflps) to identify genomic regions containing quantitative trait loci (qtl) affecting hard seededness in a segregating population from a g. max by a glycine soja (sieb. & zucc.) cross. five independent rflp markers were found to be associated with variation in the hard-seeded trait. these ... | 1990 | 24226449 |
an attempt to elucidate the origin of cultivated soybean via comparison of nucleotide sequences encoding glycinin b4 polypeptide of cultivated soybean, glycine max, and its presumed wild progenitor, glycine soja. | nucleotide sequences of cdnas encoding soybean glycinin b4 polypeptide were compared for three soybean cultivars and two introductions of wild soybean, g. soja. for three g. max cultivars, only two nucleotide substitutions were found, while g. max and g. soja nucleotide sequences had four substitutions. these data give added proof that g. max originated from g. soja. on the other hand, the time required for the accumulation of four nucleotide substitutions (calculated from the parameters of 11s ... | 1989 | 24226018 |
[nucleotide sequences encoding glycinin b4 polypeptide of the cultivated soybean glycine max and its presumed wild-type ancestor glycine soja in connection with the origin of cultivated soybean]. | nucleotide sequences of cdnas encoding soybean glycinin b4 polypeptide were compared in three soybean cultivars and two plant introductions of wild soybean glycine soja. only two nucleotide substitutions were found in three cultivars g. max, as compared with g. max and g. soja having nucleotide sequences which contain four nucleotide substitutions. these data serve as additional evidence, at molecular level, indicating the origin of g. max from g. soja. on the other hand, the time period require ... | 1989 | 2625198 |
the genomic relationship between glycine max (l.) merr. and g. soja sieb. and zucc. as revealed by pachytene chromosome analysis. | this study was conducted with the objective of determining the genomic relationship between cultivated soybean (glycine max) and wild soybean (g. soja) of the subgenus soja, genus glycine. observations on cross-ability rate, hybrid viability, meiotic chromosome pairing, and pollen fertility in f 1 hybrids of g. max × g. soja and reciprocals elucidated that both species hybridized readily and set mature putative hybrid pods, generated vigorous f1 plants, had a majority of sporocytes that showed 1 ... | 1988 | 24232348 |
screening of glycine max and glycine soja genotypes for multiple shoot formation at the cotyledonary node. | to identify genotypes which may give better plant regeneration responses in vitro, multiple shoots were induced from 155 glycine max and 13 glycine soja genotypes from maturity groups '000' to 'vii' on b5 medium supplemented with 1 or 5 μmol benzylaminopurine (bap). the average number of shoots formed show genotype specific and hormone concentration specific responses, with number of shoots ranging from 1 to 12 for different genotypes. the results were reproducible with different seed lots of th ... | 1986 | 24247952 |
discrepancies among published amino acid sequences of soybean leghemoglobins: experimental evidence against cultivar differences as the sources of the discrepancies. | despite discrepancies among charged amino acid residues in published amino acid sequences, isoelectric focusing experiments failed to detect varietal differences in soybean leghemoglobins a, c1, c2, or c3. leghemoglobins from 69 domesticated soybean (glycine max) cultivars and plant introductions and 18 wild soybean (glycine soja) plant introductions were compared; the sources included soybean cultivars used by research groups in obtaining amino acid sequences and most of the ancestors of north ... | 1985 | 4083896 |
structure and chromosomal arrangement of leghemoglobin genes in kidney bean suggest divergence in soybean leghemoglobin gene loci following tetraploidization. | we have determined the structure of one of the leghemoglobin (lb) genes of phaseolus vulgaris (kidney bean) and deduced the chromosomal arrangement of leghemoglobin genes by genomic hybridizations with lb and two other sequences, each specific to the 5' or 3' region of the soybean leghemoglobin loci. by comparing this organization with two other species of legumes, glycine max (soybean) and g. soja (wild soybean), a phylogeny of leghemoglobin gene loci was traced. the intragenic structure of the ... | 1984 | 16453577 |
fast-growing rhizobia isolated from root nodules of soybean. | fast-growing rhizobia have been isolated from soybean root nodules collected in china. these new isolates are physiologically distinct from slow-growing soybean rhizobia. they formed effective nitrogen-fixing associations with wild soybean and an unbred soybean cultivar from china, but were largely ineffective as nitrogen-fixing symbionts with common commercial cultivars of soybeans. | 1982 | 17788491 |
identity recognition in response to different levels of genetic relatedness in commercial soya bean. | identity recognition systems allow plants to tailor competitive phenotypes in response to the genetic relatedness of neighbours. there is limited evidence for the existence of recognition systems in crop species and whether they operate at a level that would allow for identification of different degrees of relatedness. here, we test the responses of commercial soya bean cultivars to neighbours of varying genetic relatedness consisting of other commercial cultivars (intraspecific), its wild proge ... | 2017 | 28280587 |
identification and analysis of nahco3 stress responsive genes in wild soybean (glycine soja) roots by rna-seq. | soil alkalinity is a major abiotic constraint to crop productivity and quality. wild soybean (glycine soja) is considered to be more stress-tolerant than cultivated soybean (g. max), and has considerable genetic variation for increasing alkalinity tolerance of soybean. in this study, we analyzed the transcriptome profile in the roots of an alkalinity tolerant wild soybean variety n24852 at 12 and 24 h after 90 mm nahco3 stress by rna-sequencing. compared with the controls, a total of 449 differe ... | 2016 | 28018382 |
structural and functional characterization of the vq protein family and vq protein variants from soybean. | proteins containing the fxxxvqxhtg or vq motif interact with wrky transcription factors. although vq proteins have been reported in several plants, knowledge about their structures, functions and evolution is still very limited. here, we report structural and functional analysis of the vq protein family from soybean. like arabidopsis homologues, soybean vq proteins bind only group i and iic wrky proteins and a substantial number of their genes are responsive to stress-associated phytohormones. o ... | 2016 | 27708406 |
a novel glycine soja homeodomain-leucine zipper (hd-zip) i gene, gshdz4, positively regulates bicarbonate tolerance and responds to osmotic stress in arabidopsis. | wild soybean (glycine soja) is a highly adaptive plant species which can grow well in saline-alkaline soils. in soybean genome, there exist about 140 hd-zip (homeodomain-leucine zipper) genes. hd-zip transcription factor family is one of the largest plant specific superfamilies and plays important roles in response to abiotic stresses. although hd-zip transcription factors have been broadly reported to be involved in plant resistance to abiotic stresses like salt and drought, their roles in resp ... | 2016 | 27553065 |
gserf6, an ethylene-responsive factor from glycine soja, mediates the regulation of plant bicarbonate tolerance in arabidopsis. | this is an original study focus on erf gene response to alkaline stress. gserf6 functions as transcription factor and significantly enhanced plant tolerance to bicarbonate (hco 3 (-) ) in transgenic arabidopsis . alkaline stress is one of the most harmful, but little studied environmental factors, which negatively affects plant growth, development and yield. the cause of alkaline stress is mainly due to the damaging consequence of high concentration of the bicarbonate ion, high-ph, and osmotic s ... | 2016 | 27125386 |
a glycine soja methionine sulfoxide reductase b5a interacts with the ca(2+) /cam-binding kinase gscbrlk and activates ros signaling under carbonate alkaline stress. | although research has extensively illustrated the molecular basis of plant responses to salt and high-ph stresses, knowledge on carbonate alkaline stress is poor and the specific responsive mechanism remains elusive. we have previously characterized a glycine soja ca(2+) /cam-dependent kinase gscbrlk that could increase salt tolerance. here, we characterize a methionine sulfoxide reductase (msr) b protein gsmsrb5a as a gscbrlk interactor by using y2h and bifc assays. further analyses showed that ... | 2016 | 27121031 |
environmental and historical determinants of patterns of genetic differentiation in wild soybean (glycine soja sieb. et zucc). | wild soybean, the direct progenitor of cultivated soybean, inhabits a wide distribution range across the mainland of east asia and the japanese archipelago. a multidisciplinary approach combining analyses of population genetics based on 20 nuclear microsatellites and one plastid locus were applied to reveal the genetic variation of wild soybean, and the contributions of geographical, environmental factors and historic climatic change on its patterns of genetic differentiation. high genetic diver ... | 2016 | 26952904 |
functional characterization of a glycine soja ca(2+)atpase in salt-alkaline stress responses. | it is widely accepted that ca(2+)atpase family proteins play important roles in plant environmental stress responses. however, up to now, most researches are limited in the reference plants arabidopsis and rice. the function of ca(2+)atpases from non-reference plants was rarely reported, especially its regulatory role in carbonate alkaline stress responses. hence, in this study, we identified the p-type ii ca(2+)atpase family genes in soybean genome, determined their chromosomal location and gen ... | 2016 | 26801329 |
genetic diversity and the conservation priority of glycine soja populations from northern china. | knowledge of the spatial patterns of genetic variation in wild populations has significant implications for in situ conservation and the determination of conservation order. to study the levels of genetic diversity, spatial genetic structures, and genetic distances in glycine soja, 11 natural populations in northern china were analyzed by estimating genetic coefficients using inter-simple sequence repeat (issr) fingerprints via mixed sampling strategies. sixteen issr primers generated 98 reprodu ... | 2015 | 26681007 |
gscml27, a gene encoding a calcium-binding ef-hand protein from glycine soja, plays differential roles in plant responses to bicarbonate, salt and osmotic stresses. | calcium, as the most widely accepted messenger, plays an important role in plant stress responses through calcium-dependent signaling pathways. the calmodulin-like family genes (cmls) encode ca2+ sensors and function in signaling transduction in response to environmental stimuli. however, until now, the function of plant cml proteins, especially soybean cmls, is largely unknown. here, we isolated a glycine soja cml protein gscml27, with four conserved ef-hands domains, and identified it as a cal ... | 2015 | 26550992 |
[distribution characteristics of soil nematodes in reclaimed land of copper-mine-tailings in different plant associations]. | a survey was carried out to investigate soil nematode communities in the plant associations of gramineae (arthraxon lanceolatus, al; imperata cylindrica, ic) and leguminosae (glycine soja, gs) in reclaimed land of copper-mine-tailings and in the plant associations of gramineae (digitaria chrysoblephara, dc-ck) of peripheral control in fenghuang mountain, tongling city. a total of 1277 nematodes were extracted and sorted into 51 genera. the average individual density of the nematodes was 590 indi ... | 2015 | 26094476 |
ectopic expression of a glycine soja myo-inositol oxygenase gene (gsmiox1a) in arabidopsis enhances tolerance to alkaline stress. | myo-inositol participates in various aspects of plant physiology, and myo-inositol oxygenase is the key enzyme of the myo-inositol oxygenation pathway. previous studies indicated that myo-inositol oxygenase may play a role in plant responses to abiotic stresses. in this study, we focused on the functional characterization of gsmiox1a, a remarkable alkaline stress-responsive gene of glycine soja 07256, based on rna-seq data. using quantitative real-time pcr, we demonstrated that gsmiox1a is rapid ... | 2015 | 26091094 |
overexpression of gsgstu13 and scmrp in medicago sativa confers increased salt-alkaline tolerance and methionine content. | tau-class glutathione s-transferases (gstus) are ubiquitous proteins encoded by a large gene family in plants, which play important roles in combating different environmental stresses. in previous studies, we constructed a glycine soja transcriptional profile, and identified three gstus (gsgstu13, gsgstu14 and gsgstu19) as potential salt-alkaline stress-responsive genes. two of them, gsgstu14 and gsgstu19, have been shown to positively regulate plant salt-alkaline tolerance. in this study, we fu ... | 2015 | 26010993 |
wild soybean roots depend on specific transcription factors and oxidation reduction related genesin response to alkaline stress. | soil alkalinity is an important environmental problem limiting agricultural productivity. wild soybean (glycine soja) shows strong alkaline stress tolerance, so it is an ideal plant candidate for studying the molecular mechanisms of alkaline tolerance and identifying alkaline stress-responsive genes. however, limited information is available about g. soja responses to alkaline stress on a genomic scale. therefore, in the present study, we used rna sequencing to compare transcript profiles of g. ... | 2015 | 25874911 |
gsskp21, a glycine soja s-phase kinase-associated protein, mediates the regulation of plant alkaline tolerance and aba sensitivity. | plant skp1-like family proteins, components of the scf complex e3 ligases, are involved in the regulation of plant development and stress responses. little is known about the precise function of skp genes in plant responses to environmental stresses. gsskp21 was initially identified as a potential stress-responsive gene based on the transcriptome sequencing of glycine soja. in this study, we found that gsskp21 protein contains highly conserved skp domains in its n terminus and an extra unidentif ... | 2015 | 25477077 |
comparison of saponin composition and content in wild soybean (glycine soja sieb. and zucc.) before and after germination. | eight wild soybean accessions with different saponin phenotypes were used to examine saponin composition and relative saponin quantity in various tissues of mature seeds and two-week-old seedlings by lc-pda/ms/ms. saponin composition and content were varied according to tissues and accessions. the average total saponin concentration in 1 g mature dry seeds of wild soybean was 16.08 ± 3.13 μmol. in two-week-old seedlings, produced from 1 g mature seeds, it was 27.94 ± 6.52 μmol. group a saponins ... | 2014 | 25127168 |
identification of quantitative trait loci controlling linolenic acid concentration in pi483463 (glycine soja). | the qtls controlling alpha-linolenic acid concentration from wild soybean were mapped on nine soybean chromosomes with various phenotypic variations. new qtls for alpha-linolenic acid were detected in wild soybean. alpha-linolenic acid (ala) is a polyunsaturated fatty acid desired in human and animal diets. some wild soybean (glycine soja) genotypes are high in ala. the objective of this study was to identify quantitative trait loci (qtls) controlling ala concentration in a wild soybean accessio ... | 2014 | 24794978 |
allelic variation of soybean flower color gene w4 encoding dihydroflavonol 4-reductase 2. | flower color of soybean is primarily controlled by six genes, viz., w1, w2, w3, w4, wm and wp. this study was conducted to investigate the genetic and chemical basis of newly-identified flower color variants including two soybean mutant lines, 222-a-3 (near white flower) and e30-d-1 (light purple flower), a near-isogenic line (clark-w4), flower color variants (t321 and t369) descended from the w4-mutable line and kw4 (near white flower, glycine soja). | 2014 | 24602314 |
ectopic expression of gsppck3 and scmrp in medicago sativa enhances plant alkaline stress tolerance and methionine content. | so far, it has been suggested that phosphoenolpyruvate carboxylases (pepcs) and pepc kinases (ppcks) fulfill several important non-photosynthetic functions. however, the biological functions of soybean ppcks, especially in alkali stress response, are not yet well known. in previous studies, we constructed a glycine soja transcriptional profile, and identified three ppck genes (gsppck1, gsppck2 and gsppck3) as potential alkali stress responsive genes. in this study, we confirmed the induced expre ... | 2014 | 24586886 |
physiological mechanisms for high salt tolerance in wild soybean (glycine soja) from yellow river delta, china: photosynthesis, osmotic regulation, ion flux and antioxidant capacity. | glycine soja (bb52) is a wild soybean cultivar grown in coastal saline land in yellow river delta, china. in order to reveal the physiological mechanisms adapting to salinity, we examined photosynthesis, ion flux, antioxidant system and water status in glycine soja under nacl treatments, taking a cultivated soybean, zh13, as control. upon nacl exposure, higher relative water content and water potential were maintained in the leaf of bb52 than zh13, which might depend on the more accumulation of ... | 2013 | 24349468 |
soybean knowledge base (soykb): a web resource for integration of soybean translational genomics and molecular breeding. | soybean knowledge base (http://soykb.org) is a comprehensive web resource developed for bridging soybean translational genomics and molecular breeding research. it provides information for six entities including genes/proteins, micrornas/srnas, metabolites, single nucleotide polymorphisms, plant introduction lines and traits. it also incorporates many multi-omics datasets including transcriptomics, proteomics, metabolomics and molecular breeding data, such as quantitative trait loci, traits and ... | 2014 | 24136998 |
ectopic expression of a wrky homolog from glycine soja alters flowering time in arabidopsis. | flowering is a critical event in the life cycle of plants; the wrky-type transcription factors are reported to be involved in many developmental processes sunch as trichome development and epicuticular wax loading, but whether they are involved in flowering time regulation is still unknown. within this study, we provide clear evidence that gswrky20, a member of wrky gene family from wild soybean, is involved in controlling plant flowering time. expression of gswrky20 was abundant in the shoot ti ... | 2013 | 23991184 |
overexpression of gszfp1 enhances salt and drought tolerance in transgenic alfalfa (medicago sativa l.). | gszfp1 encodes a cys2/his2-type zinc-finger protein. in our previous study, when gszfp1 was heterologously expressed in arabidopsis, the transgenic arabidopsis plants exhibited enhanced drought and cold tolerance. however, it is still unknown whether gszfp1 is also involved in salt stress. gszfp1 is from the wild legume glycine soja. therefore, the aims of this study were to further elucidate the functions of the gszfp1 gene under salt and drought stress in the forage legume alfalfa and to inves ... | 2013 | 23867600 |
expression of wild soybean wrky20 in arabidopsis enhances drought tolerance and regulates aba signalling. | the wrky-type transcription factors are involved in plant development and stress responses, but how the regulation of stress tolerance is related to plant development is largely unknown. gswrky20 was initially identified as a stress response gene using large-scale glycine soja microarrays. quantitative reverse transcription-pcr (qrt-pcr) showed that the expression of this gene was induced by abscisic acid (aba), salt, cold, and drought. overexpression of gswrky20 in arabidopsis resulted in a dec ... | 2013 | 23606412 |
a glycine soja aba-responsive receptor-like cytoplasmic kinase, gsrlck, positively controls plant tolerance to salt and drought stresses. | receptor such as protein kinases are proposed to work as sensors to initiate signaling cascades in higher plants. however, little is known about the precise functions of receptor such as protein kinases in abiotic stress response in plants, especially in wild soybean. here, we focused on characterization of the biological functions of a receptor-like cytoplasmic serine/threonine protein kinase gene, gsrlck, which was previously identified as a putative salt-alkali stress-related gene from the tr ... | 2013 | 23494614 |
gssrk, a g-type lectin s-receptor-like serine/threonine protein kinase, is a positive regulator of plant tolerance to salt stress. | receptor-like protein kinases (rlks) play vital roles in sensing outside signals, yet little is known about rlks functions and roles in stress signal perception and transduction in plants, especially in wild soybean. through the microarray analysis, gssrk was identified as an alkaline (nahco3)-responsive gene, and was subsequently isolated from glycine soja by homologous cloning. gssrk encodes a 93.22kda protein with a highly conserved serine/threonine protein kinase catalytic domain, a g-type l ... | 2013 | 23276523 |
genetic diversity and population structure: implications for conservation of wild soybean (glycine soja sieb. et zucc) based on nuclear and chloroplast microsatellite variation. | wild soybean (glycine soja sieb. et zucc) is the most important germplasm resource for soybean breeding, and is currently subject to habitat loss, fragmentation and population decline. in order to develop successful conservation strategies, a total of 604 wild soybean accessions from 43 locations sampled across its range in china, japan and korea were analyzed using 20 nuclear (nssrs) and five chloroplast microsatellite markers (cpssrs) to reveal its genetic diversity and population structure. r ... | 2012 | 23202917 |
tracing soybean domestication history: from nucleotide to genome. | since the genome sequences of wild species may provide key information about the genetic elements involved in speciation and domestication, the undomesticated soybean (glycine soja sieb. and zucc.), a wild relative of the current cultivated soybean (g. max), was sequenced. in contrast to the current hypothesis of soybean domestication, which holds that the current cultivated soybean was domesticated from g. soja, our previous work has suggested that soybean was domesticated from the g. soja/g. m ... | 2012 | 23136484 |
identification of wild soybean mirnas and their target genes responsive to aluminum stress. | micrornas (mirnas) play important regulatory roles in development and stress response in plants. wild soybean (glycine soja) has undergone long-term natural selection and may have evolved special mechanisms to survive stress conditions as a result. however, little information about mirnas especially mirnas responsive to aluminum (al) stress is available in wild soybean. | 2012 | 23040172 |
over-expression of a novel jaz family gene from glycine soja, increases salt and alkali stress tolerance. | salt and alkali stress are two of the main environmental factors limiting crop production. recent discoveries show that the jaz family encodes plant-specific genes involved in jasmonate signaling. however, there is only limited information about this gene family in abiotic stress response, and in wild soybean (glycine soja), which is a species noted for its tolerance to alkali and salinity. here, we isolated and characterized a novel jaz family gene, gsjaz2, from g. soja. transcript abundance of ... | 2012 | 22943855 |
comparative genomic analysis of soybean flowering genes. | flowering is an important agronomic trait that determines crop yield. soybean is a major oilseed legume crop used for human and animal feed. legumes have unique vegetative and floral complexities. our understanding of the molecular basis of flower initiation and development in legumes is limited. here, we address this by using a computational approach to examine flowering regulatory genes in the soybean genome in comparison to the most studied model plant, arabidopsis. for this comparison, a gen ... | 2012 | 22679494 |
gsapk, an aba-activated and calcium-independent snrk2-type kinase from g. soja, mediates the regulation of plant tolerance to salinity and aba stress. | plant snf1 (sucrose non-fermenting-1) related protein kinase (snrk), a subfamily of serine/threonine kinases, has been implicated as a crucial upstream regulator of aba and osmotic signaling as in many other signaling cascades. in this paper, we have isolated a novel plant specific aba activated calcium independent protein kinase (gsapk) from a highly salt tolerant plant, glycine soja (50109), which is a member of the snrk2 family. subcellular localization studies using gfp fusion protein indica ... | 2012 | 22439004 |
[isolation and functional analysis of gstify11b relevant to salt and alkaline stress from glycine soja]. | using homologous cloning and rt-pcr technology, we isolated a novel tify family gene, gstify11b, from glycine soja l. g07256, a species that is tolerant to saline and alkaline environments. phylogenetic analysis indicated that gstify11b was closely related to attify11a with 56% similarity in amino acid identity. protein sequence analysis showed that gstify11b protein also had conserved tify domain, n-terminal domain, and a c-terminal jas motif. quantitative realtime pcr analysis indicated that t ... | 2012 | 22382065 |
[study of interaction of wild soybean subpopulations (glycine soja) in the valley of the tsukanovka river in the south of far east of russia]. | a comparative study of the genetic structure of natural and anthropogenic populations of g. soja gives significant information about formation of different populations, and allows developing measures for preservation of unique natural gene bank of wild soybean, the species closely related to cultivated soybean. in this study, issr markers were used to carry out a comparative analysis of genetic structure of natural and anthropogenic subpopulations of g. soja for studying possible mutual influenc ... | 2011 | 21950138 |
gsgasa1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of dellas. | low, but not freezing temperatures are a major factor limiting plant vegetative growth and development. it is not clear what signaling mechanism is used to limit plant growth in response to chronic low temperatures, thus it is important to continue to isolate and characterize genes whose activity/expression correlate well with cold responses. in this study, a novel gasa gene was isolated from glycine soja and named gsgasa1. quantitative real-time pcr analysis indicated that gsgasa1 expression re ... | 2011 | 21855169 |
secondary metabolites from glycine soja and their growth inhibitory effect against spodoptera litura. | the wild soybean (glycine soja sieb. et zucc) has been reported to be relatively resistant to insect and pathogenic pests. however, the responsible secondary metabolites in the aerial part of this important plant are largely unknown. from the aerial part of g. soja, 13 compounds were isolated and identified, including seven isoflavonoids (1-7), a cyclitol (8), two sterol derivatives (9 and 10), and three triterpenoids (11-13). compound 7 is a new isoflavonoid, and compounds 9 and 10 are reported ... | 2011 | 21545185 |
[study of genetic diversity and spatial structure of the wild soybean (glycine soja sieb. & zucc.) population from the ekaterinovka in the south of primorskii krai]. | data are presented on the genetic diversity and spatial structure of the natural wild soybean population from the neighborhood of the settlement of ekaterinovka in primorskii krai and on the relationship between the genetic structure of this population and its spatial organization. these data are discussed in comparison with the results of studies of wild soybean populations in the far east region of the russian federation and china. recommendations are given concerning the collection of genetic ... | 2011 | 21542304 |
treatment of bromhidrosis with a glycine-soja sterocomplex topical product. | bromhidrosis is a common problem with a severe negative impact on the patient's social and emotional life. the objective of this open-label study was to evaluate the efficacy and safety of a local product with the main active ingredient of glycine-soja sterocomplex in the treatment of local bromhidrosis. | 2011 | 21332920 |
alkaline-stress response in glycine soja leaf identifies specific transcription factors and aba-mediated signaling factors. | transcriptome of glycine soja leaf tissue during a detailed time course formed a foundation for examining transcriptional processes during nahco(3) stress treatment. of a total of 2,310 detected differentially expressed genes, 1,664 genes were upregulated and 1,704 genes were downregulated at various time points. the number of stress-regulated genes increased dramatically after a 6-h stress treatment. go category gene enrichment analysis revealed that most of the differentially expressed genes w ... | 2011 | 20938706 |
phenolics in the seed coat of wild soybean (glycine soja) and their significance for seed hardness and seed germination. | hardseededness in annual wild soybean (glycine soja sieb. et zucc.) is a valuable trait that affects the germination, viability, and quality of stored seeds. two g. soja ecotypes native to shandong province of china have been used to identify the phenolics in the seed coat that correlate with the seed hardness and seed germination. three major phenolics from the seed coat were isolated and identified as epicatechin, cyanidin 3-o-glucoside, and delphinidin 3-o-glucoside. of the three phenolics, o ... | 2010 | 20879792 |
a new allele of flower color gene w1 encoding flavonoid 3'5'-hydroxylase is responsible for light purple flowers in wild soybean glycine soja. | glycine soja is a wild relative of soybean that has purple flowers. no flower color variant of glycine soja has been found in the natural habitat. | 2010 | 20663233 |
global transcriptome profiling of wild soybean (glycine soja) roots under nahco3 treatment. | plant roots are the primary site of perception and injury for saline-alkaline stress. the current knowledge of saline-alkaline stress transcriptome is mostly focused on saline (nacl) stress and only limited information on alkaline (nahco3) stress is available. | 2010 | 20653984 |
gscbrlk, a calcium/calmodulin-binding receptor-like kinase, is a positive regulator of plant tolerance to salt and aba stress. | calcium/calmodulin-dependent kinases play vital roles in protein phosphorylation in eukaryotes, yet little is known about the phosphorylation process of calcium/calmodulin-dependent protein kinase and its role in stress signal transduction in plants. a novel plant-specific calcium-dependent calmodulin-binding receptor-like kinase (gscbrlk) has been isolated from glycine soja. a subcellular localization study using gfp fusion protein indicated that gscbrlk is localized in the plasma membrane. bin ... | 2010 | 20400529 |
identification of micrornas in wild soybean (glycine soja). | micrornas (mirnas) play important roles in post-transcriptional gene silencing by directing target mrna cleavage or translational inhibition. currently, hundreds of mirnas have been identified in plants, but no report has been published of wild soybean (glycine soja sieb). we constructed a small-rna library consisting of 2 880 sequences with high quality, in which 1 347 were 19-24 nt in length. by utilizing the mirna, rfam and domesticated soybean expressed sequence tag database, we have analyze ... | 2009 | 20021554 |
fine-scale genetic structure enhances biparental inbreeding by promoting mating events between more related individuals in wild soybean (glycine soja; fabaceae) populations. | outcrossing between genetically distant individuals in a plant population enhances allelic heterozygosity-an important source for genetic diversity and adaptive evolution. fine-scale spatial genetic structure (fsgs) can interfere with outcrossing by promoting mating between more related individuals. to test the influence of fsgs on outcrossing, fsgs and outcrossing rates were analyzed with four wild soybean (glycine soja) populations from different habitats, using simple sequence repeat (ssr) fi ... | 2009 | 21628264 |
[molecular genetic analysis of wild soybean (glycine soja sieb. & zucc.) population structure in anthropogenic and natural landscapes of primorskii krai]. | the data are presented on genetic population structure of wild soybean growing in natural and anthropogenically disturbed landscapes of primorskii krai of the russian federation. comparative analysis showed that wild soybean populations exposed to anthropogenic influence exhibited lower genetic diversity than natural populations. recommendations on conservation of the wild plant gene pools using comparative data on population genetic structures are made. | 2008 | 18825957 |
effect of salt stress on seed germination and seedling growth of three salinity plants. | seeds of three halophyte species included limonium sinense kuntze, glycine soja sieb. and sorghum sudanense stapf. were used to investigate the effects of different salinity concentrations on their germination percentage, germination energy, germination index, relative germination rate, relative salt-injury rate, radicle length and hypocotyl length. results showed that seeds of g. soja can germinate well and rapidly at lower level of salt (< 200 mmol l(-1)), 50 mmol l(-1) salt concentrations is ... | 2008 | 18819537 |
proteomic and genetic analysis of glycinin subunits of sixteen soybean genotypes. | we investigated proteomic and genomic profiles of glycinin, a family of major storage proteins in 16 different soybean genotypes consisting of four groups including wild soybean (glycine soja), unimproved cultivated soybean landraces from asia (g. max), ancestors of n. american soybean (g. max), and modern soybean (g. max) genotypes. we observed considerable variation in all five glycinin subunits, g1, g2 g3, g4 and g5 using proteomics and genetic analysis. two-dimensional polyacrylamide gel ele ... | 2016 | 17524657 |
proteomic and genomic characterization of kunitz trypsin inhibitors in wild and cultivated soybean genotypes. | in this study, we investigated protein and genetic profiles of kunitz trypsin inhibitors (ktis) in seeds of 16 different soybean genotypes that included four groups consisting of wild soybean (glycine soja), the cultivated soybean (g. max) ancestors of modern n. american soybean cultivars (old), modern n. american soybean (elite), and asian cultivated soybean landraces that were the immediate results of domestication from the wild soybean. proteins were well separated by two-dimensional polyacry ... | 2007 | 16884824 |
genetic characterization of a novel tib-derived variant of soybean kunitz trypsin inhibitor detected in wild soybean (glycine soja). | a novel variant of soybean kunitz trypsin inhibitor (skti) was detected in 530 lines of wild soybean (glycine soja). this variant showed an intermediate electrophoretic mobility between the tia and tic types. in isoelectric focusing polyacrylamide gel electrophoresis gels containing urea, this variant had a similar isoelectric point as that of tia. the genetic analysis of skti bands in f2 seeds from crosses of the new variant type with tia or tic type showed that this variant type is controlled ... | 2004 | 15060597 |
evolutionary changes in the structures of the cox2 and atp6 loci in the mitochondrial genome of soybean involving recombination across small interspersed sequences. | mitochondrial dna (mtdna) fragments that contained cox2 or atp6 loci were cloned from three accessions of wild soybean (glycine soja) in order to understand the evolutionary changes of mitochondrial genomes in the genus glycine subgenus soja. cox2 was cloned as a single configuration, while atp6 was cloned as either one or two configurations from each accession. structural variations were detected in the 5' upstream region of cox2 and in both the 5' upstream and 3' downstream regions of atp6. th ... | 1998 | 9799364 |
small interspersed sequences that serve as recombination sites at the cox2 and atp6 loci in the mitochondrial genome of soybean are widely distributed in higher plants. | mitochondrial dna (mtdna) fragments that contain cox2 and atp6 were cloned from a wild soybean (glycine soja, accession 'b09002') and from a cultivated soybean (g. max, 'harosoy'). comparison of these dnas revealed that two sets of repeated sequences, namely, 299 bp and 23 bp, were present in the 5' regions of cox2 and atp6. the 299-bp and 23-bp repeats were present close to each other on the 5' flanking region and the 5' part of the coding region of cox2 in both 'harosoy' and 'b09002', as well ... | 1998 | 9508793 |
cooperative action of lipo-chitin nodulation signals on the induction of the early nodulin, enod2, in soybean roots. | various lipo-chitin molecules were tested for their ability to induce the expression of the early nodulin, enod2, in glycine soja roots. when inoculated separately onto g. soja roots, lco-v (c18:1 delta 11,mefuc), lco-v (c18:1 delta 9,mefuc), lco-v (c16:0,mefuc), and lco-iv (c16:0) were unable to induce enod2 expression, even though these compounds had previously been shown to induce root hair curling, the formation of nodule-like primordia, and induction of the early nodulin, enod40. enod2 expr ... | 1996 | 8810072 |
microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean. | the objectives of this study were to (i) assess the extent of genetic variation in soybean microsatellites (simple sequence repeats or ssrs), (ii) assay for amplified sequence length polymorphisms (aslps), and (iii) evaluate the usefulness of ssrs and aslps as genetic markers. five microsatellites detected a total of 79 variants (alleles) in a sample of 94 accessions of wild (glycine soja) and cultivated soybean (g. max). f2 segregation analysis of four of the five microsatellites identified the ... | 1995 | 7672605 |
the glycinin a3b4 mrna from wild soybean glycine soja sieb. et zucc. | | 1995 | 7724684 |
nucleotide sequence of the 5s rrna gene from glycine soja. | | 1992 | 1511128 |
isolation, purification, and partial characterization of formate dehydrogenase from soybean seed. | soybean (glycine soja var beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. the enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. it has activity over a broad ph range, is stable for months at 4 degrees c, and has k(m) values of 0.6 millimolar and 5.7 micromolar for formate and nad, respectively. | 1983 | 16663314 |
a case of polyagglutination with features of th and tk activation associated with an acquired b antigen. | a case is reported of polyagglutination with features of both tk and th activation associated with an acquired b antigen. reactions with polybrene, glycine soja, and bandeiraea simplicifolia ii indicated the presence of tk activation. however, the reactivity of the patient's erythrocytes with arachis hypogoea was greatly reduced by treatment with ficin, indicating the concomitant presence of th activation. | 2004 | 6829061 |
studies on lectins. xlix. the use of glycosyl derivatives of dextran t-500 for affinity electrophoresis of lectins. | p-aminophenyl glycosides and glycosylamines were coupled to periodate oxidized dextran t-500 either directly or through an epsilon-aminocaproic acid spacer. the new glycosylated derivatives of dextran specifically precipitate lectins having the appropriate carbohydrate specificity, and thus were used in the preparation of affinity gels for affinity electrophoresis of lectins. the apparent strength of interaction of several lectins with carbohydrate residues immobilized in this way was less than ... | 1980 | 6161150 |
studies on lectins. xliv. the ph dependence of lectin interactions with sugars as determined by affinity electrophoresis. | the ph dependence of association constants of the lectin-sugar complexes was determined by means of affinity electrophoresis. all the lectins studied (from the seeds of dolichos biflorus, glycine soja, lens esculenta and vicia cracca and of the fruiting body of marasmius oreades) were characterized by a similar course of ph dependence of the association constants, with the maximum values at ph 7--9. for concanavalin a and the l-fucose binding ulex europaeus lectin only the association constants ... | 1979 | 33723 |
influence of enzyme and substrate concentrations on the decomposition of urea by commercial urease and urease obtained from glycine soja beans in phosphate buffer. | | 1961 | 13685889 |
studies on the influence of stannous chloride on the decomposition of urea by soyabean (glycine soja) urease. | | 1960 | 13795787 |
haemagglutinins of glycine soja. | | 1955 | 13272759 |
crossing experiments with glycine soja and g. gracilis. | | 1952 | 12989433 |
rna-seq data comparisons of wild soybean genotypes in response to soybean cyst nematode (heterodera glycines). | soybean [glycine max (l.) merr.] is an important crop rich in vegetable protein and oil, and is a staple food for human and animals worldwide. however, soybean plants have been challenged by soybean cyst nematode (scn, heterodera glycines), one of the most damaging pests found in soybean fields. applying scn-resistant cultivars is the most efficient and environmentally friendly strategy to manage scn. currently, soybean breeding and further improvement in soybean agriculture are hindered by seve ... | 2017 | 28856099 |