| purification and characterization of trypsins from the digestive tract of locusta migratoria. | two trypsin-like enzymes were isolated from the digestive tract of the african migratory locust locusta migratoria migratorioides. primary purification was carried out on a deae-cellulose column, from which the two trypsins emerged in the anionic fraction. further purification was achieved by affinity chromatography on a p-aminobenzamidine (paba)-sepharose column, which also separated the two trypsins (tleaff.1. and tleaff.2.), or by hplc on an anion exchange column. the purity and homogeneity o ... | 1989 | 2635697 |
| fusarium moniliforme sheld. a fungus producing a broad spectrum of bioactive metabolites. | fusarium moniliforme sheldon (perfect stage gibberella fujikuroi (saw. wollenweber), an ubiquitous fungus belonging to the section liseola (booth), is one of the widespread, phytopathogenic fungi. this field fungus common on many crops, including rice, oats, wheat, maize, barley and soybeans was well known because of the ability to produce gibberellins in high quantities. today beside of the very important gibberellic acid (ga3) other gibberellins such as ga4 and ga7 are of significance for agri ... | 1989 | 2652945 |
| estimation of nitrogenase activity in the presence of ethylene biosynthesis by use of deuterated acetylene as a substrate. | nitrogenase reduces deuterated acetylene primarily to cis dideuterated ethylene. this can be distinguished from undeuterated ethylene by the use of fourier transform infrared spectroscopy. characteristic bands in the region from 800 to 3,500 cm-1 can be used to identify and quantitate levels of these products. this technique is applicable to field studies of nitrogen fixation where ethylene biosynthesis by plants or bacteria is occurring. we have verified the reaction stoichiometry by using kleb ... | 1989 | 2655535 |
| [evaluation of allergen-specific ige antibodies by a newly developed mast allergy system]. | mast, which stand for multiple antigen simultaneous test, uses enzyme-linked anti-human ige and chemiluminogenic substate to determine ige. this system is characterized by simultaneous analysis of multiple allergen items, up to 35, together with total ige determination. we evaluated usefulness of this mast system using 191 serum samples obtained from patients with bronchial asthma, allergic rhinitis and/or atopic dermatitis. it was found that there were statistically significant correlations bet ... | 1989 | 2686587 |
| lectin-binding sites in neoplastic and non-neoplastic colonic mucosa of 1,2-dimethylhydrazine-treated rats. | qualitative changes of glycoconjugates in luminal surface and goblet cell mucin from colon mucosa of 1,2-dimethylhydrazine (dmh)-treated rats were studied. eight fluoresceinated lectins were used: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), limax flavus (lfa), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), ulex europaeus-i (uea-i) and canavalia ensiformis (con a). the lectin-binding patterns were studied in tumors arising in proximal and distal portions of the ... | 1989 | 2689774 |
| apparent digestibility of nitrogen and amino acids in protein feedstuffs by growing pigs. | eight digestion trials were conducted with pigs fitted with ileal t-cannulas to determine the apparent digestibilities of n and amino acids in common protein feedstuffs. trial-to-trial variation was minimal as determined by the variation in digestibilities for soybean meal, which was evaluated in each trial. apparent digestibilities varied widely; ileal n digestibility ranged from 88 to 63%, and ileal lysine digestibility ranged from 93 to 40%. in general, ring-dried blood meal, corn gluten meal ... | 1989 | 2703444 |
| the major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species. | several plant species are known to synthesize low-molecular-weight nucleus-encoded heat shock proteins (hsps) which localize to chloroplasts. dna sequence analysis of chloroplast hsp cdnas from pea (pisum sativum) and soybean (glycine max) has shown that the carboxyl-terminal halves of these proteins are homologous to low-molecular-weight hsps from a wide range of eucaryotes (e. vierling, r. t. nagao, a. e. derocher, and l. m. harris, embo j. 7:575-581, 1988). we used a pea cdna to construct fus ... | 1989 | 2710111 |
| rgd-dependent linkage between plant cell wall and plasma membrane: consequences for growth. | soybean (glycine max [l.] merr. cv. mandarin) root cells (sb-1 cell line) grown in suspension culture containing glycyl-arginyl-glycyl-aspartyl-seryl-proline (grgdsp) (0.25 mg/ml), a synthetic peptide containing the rgd sequence found in many extracellular matrix adhesive proteins, demonstrated (a) significantly enhanced growth rate, and (b) aberrant cell wall/plasma membrane interactions and organization. substitution of the asp (d) by a glu (e) amino acid in the hexapeptide, or inversion of th ... | 1989 | 2715184 |
| characterization and solubilization of cyclo(his-pro) binding from rat liver membranes. | we have found cyclo(his-pro) binding in rat liver plasma membranes. this study focused on the characterization of solubilized binding for cyclo(his-pro) in rat liver membranes. the cyclo(his-pro) binding of liver membranes was solubilized by digitonin and octyl-glucopyranoside. the efficiency of solubilization with digitonin was greater. however, cyclo(his-pro) binding was not solubilized by triton x-100, chaps, or lubrol. digitonin-solubilized membranes showed cyclo(his-pro) binding with a high ... | 1989 | 2726776 |
| demonstration of sugar residues in the ultimobranchial tubule and thyroid c-cells of the rat using peroxidase labelled lectins. | the distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid c-cells has been studied, using eight peroxidase-labelled lectins (dolichos biblorus, glycine max, bauhinia purpurea, helix pomatia, arachis hipogaea, ulex europaeus i, limax flavus and sophora japonica). the results obtained seem to show that there are receptors for dolichos biflorus, glycine max, bauhinia purpurea, helix pomatia ... | 1989 | 2757235 |
| ruminal metabolism in nonlactating cows fed whole cottonseed or extruded soybeans. | five nonlactating holstein cows (average weight 574 kg) with cannulas in the rumen and duodenum were fed five total mixed diets at 2.14% (dm) of bw for seven 2-wk periods to estimate the ruminal degradation of protein and fatty acids in whole cottonseed (wcs) and extruded soybeans (esb). lanthanum was used as an indigestible marker. ruminal propionic acid (molar proportion) was larger and butyric acid was smaller for wcs diets than for control or esb diets. total vfa (mm) and acetic acid (molar ... | 1989 | 2768119 |
| an immunologically related family of apolipoproteins associated with triacylglycerol storage in the cruciferae. | the major apolipoproteins associated with oil-storage bodies have been isolated from the mature seeds of six different species of the family cruciferae. the apolipoproteins were all of molecular mass 19-20 kda. they were highly abundant in mature seed tissue, accounting for up to 20% total seed proteins, and were localized exclusively on the membranes of oil-storage bodies. antibodies were raised in rabbits and mice against the six purified apolipoproteins. in each case, the antibodies specifica ... | 1989 | 2774566 |
| changes in lectin binding of lumbar dorsal root ganglia neurons and peripheral axons after sciatic and spinal nerve injury in the rat. | the effects of chronic lesions of rat lumbar spinal or sciatic nerves on the binding of glycine max (soybean) agglutinin to galacto-conjugates, in small- and medium-size primary sensory neurons of the l4 and l5 dorsal root ganglia, were examined over a 580-day period. spinal nerve section resulted in a marked decrease in the population of stained neurons within 7 days. however, despite some retrograde morphological changes triggered by axonal injury, the proportion of stained nerve cells was nor ... | 1989 | 2776185 |
| a cacgtg motif of the antirrhinum majus chalcone synthase promoter is recognized by an evolutionarily conserved nuclear protein. | in the chalcone synthase gene of antirrhinum majus (snapdragon), 150 base pairs of the 5' flanking region contain cis-acting signals for uv light-induced expression. a nuclear factor, designated cg-1, specifically recognizes a hexameric motif with internal dyad symmetry, cacgtg, located within this light-responsive sequence. binding of cg-1 is influenced by c-methylation of the cpg dinucleotide in the recognition sequence. cg-1 is a factor found in a variety of dicotyledonous plant species inclu ... | 1989 | 2780550 |
| studies of lectin binding to the human cervix uteri: i. normal cervix. | lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), arachis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga) were used to evaluate cell surface carbohydrates in formalin-fixed paraffin-embedded tissue sections of normal human cervix uteri. consistent patterns of staining of the squamous epithelium were obtained in all 30 cases with bpa, gs ii, mpa, pn ... | 1989 | 2793521 |
| studies of lectin binding to the human cervix uteri: ii. cervical intraepithelial neoplasia and invasive squamous carcinoma. | the cell surface carbohydrate profile of formalin-fixed paraffin-embedded tissue sections of neoplastic cervical squamous epithelium was evaluated using lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), archis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga). three lectins (bpa, con a and pna) showed a similar pattern of staining in both normal squa ... | 1989 | 2793522 |
| bradyrhizobium japonicum glnb, a putative nitrogen-regulatory gene, is regulated by ntrc at tandem promoters. | the glnb gene from bradyrhizobium japonicum, the endosymbiont of soybeans (glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. the b. japonicum glnb gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnb gene products from klebsiella pneumoniae and escherichia coli. the gene is located directly upstream from glna (encoding glutamine synthetase), a linkage not observed in enteric bacteria. the g ... | 1989 | 2793830 |
| rhizobium japonicum usda 191 has two nodd genes that differ in primary structure and function. | several rhizobium genes (designated nod genes) are involved in early steps in nodule formation. here we present the results of dna sequence and functional analysis of two nodd genes from the symbiotic plasmid of usda 191, a fast-growing strain that forms nitrogen-fixing nodules on soybeans. both genes encoded full-length nodd-related polypeptides, which were 69% homologous to each other. one of these genes, nodd1, complemented a rhizobium trifolii nodd::tn5 mutant for clover nodulation; the othe ... | 1988 | 2826389 |
| heterogeneity between soluble human and rabbit splenic alpha 2-adrenoceptors. | the pharmacological and biochemical characteristics of soluble alpha 2-adrenoceptors were investigated to determine whether differences observed in membranes were maintained in solution and to probe the nature of any such differences. alpha 2-adrenoceptors were solubilized from purified plasma membrane preparations of human and rabbit spleen using digitonin. [3h]yohimbine bound to one population of alpha 2-adrenoceptors in the preparations with dissociation constants of 2.4 nm and 7.8 nm respect ... | 1986 | 2876709 |
| role of the bradyrhizobium japonicum ntrc gene product in differential regulation of the glutamine synthetase ii gene (glnii). | we isolated the ntrc gene from bradyrhizobium japonicum, the endosymbiont of soybean (glycine max), and examined its role in regulating nitrogen assimilation. two independent ntrc mutants were constructed by gene replacement techniques. one mutant was unable to produce ntrc protein, while the other constitutively produced a stable, truncated ntrc protein. both ntrc mutants were unable to utilize potassium nitrate as a sole nitrogen source. in contrast to wild-type b. japonicum, the ntrc null mut ... | 1988 | 2903856 |
| effect of supplemental niacin or niacinamide and soybean source on ruminal bacterial fermentation in continuous culture. | effects of niacin or niacinamide in diets containing either soybean meal, raw whole soybeans or whole soybeans extruded at 132 and 149 c on ruminal bacterial fermentation were examined with a dual-flow continuous culture system. in exp. 1, soybean sources each provided 50% of total crude protein in diets comprised of 52% concentrate mix, 36% corn silage and 12% alfalfa hay (dry-matter basis). each diet was supplemented with 0 or 100 mg/kg niacin. niacin supplementation increased (p less than .05 ... | 1985 | 2934360 |
| identification of a nodd-dependent locus in the rhizobium strain ngr234 activated by phenolic factors secreted by soybeans and other legumes. | transfer of the strain ngr234nodd 1 gene into the narrow host range r. trifolii strain anu843 on either a 6.7-kb hindiii or 17-kb xhoi fragment broadens the host range of this bacterium to include the tropical legumes vigna unguiculata, glycine ussuriensis, leucaena leucocephala, and siratro (macroptilium atropurpureum). contrary to previous data (bassam et al. 1986), mutagenesis of the 17-kb xhoi fragment with a mini-mu lac transposon (mu dii1734) showed that a functional nodd 1 gene was essent ... | 1988 | 2980201 |
| slow-growing rhizobium japonicum comprises two highly divergent symbiotic types. | we examined the interrelationships of the genomes of 10 slow-growing strains of rhizobium japonicum to provide a foundation for molecular genetic studies of these agriculturally important endosymbiotic bacteria of commercial soybeans. the degree of base substitution in and around known symbiotic genes (nif and presumptive nod), constitutively expressed genes (glna and reca), and two other cloned sequences was estimated from restriction site variation by using cloned dnas as hybridization probes ... | 1985 | 2989244 |
| expression of symbiotic genes of rhizobium japonicum usda 191 in other rhizobia. | a 200-megadalton plasmid was mobilized from rhizobium japonicum usda 191 to other rhizobium strains either that cannot nodulate soybeans or that form fix- nodules on certain cultivars. the symbiotic properties of the transconjugants indicate that both soybean specificity for nodulation and cultivar specificity for nitrogen fixation are plasmid encoded. | 1985 | 2989250 |
| proton motive force in washed cells of rhizobium japonicum and bacteroids from glycine max. | the components of the proton motive force (delta p), namely the membrane potential and the transmembrane ph gradient, were measured in washed cells of rhizobium japonicum cc705 grown in cultures (5% o2-95% n2) in the presence of 10 mm kno3 and in bacteroids from glycine max. the delta p and its components remained reasonably constant in cells as well as in bacteroids at various stages of growth. the effects of uncouplers and atpase inhibitors on the delta p and its components were determined in ... | 1985 | 2999086 |
| comparison of the effect of cell wall and hull fiber from canola and soybean on the bioavailability for rats of minerals, protein and lipid. | hull or cell wall material, isolated from canola (brassica napus cv. regent) or soybean (glycine max) or cellulose was added to a basal, semipurified diet at a level of 12% and fed to growing male wistar rats. the apparent availability of cu, fe, ca, p and protein were lower when the fiber-containing diets were fed compared to the control diet. the availability of mg was lower when the canola hull (ch) and cellulose (ce) diets were fed, whereas zn availability was lower when the canola cell wall ... | 1986 | 3003294 |
| lectin binding to elastic fibres and associated components during development of the human aorta. | a panel of peroxidase-conjugated lectins was used to stain frozen sections of human foetal, neonatal, infant and adult aortae. two main distinctive staining patterns were observed at the light microscope level, depending on the lectin employed. lectins from concanavalin a, triticum vulgaris, arachis hypogaea and glycine max resulted in staining of the surfaces of aortic elastic lamellae and the interlamellar areas. with lectins from dolichos biflorus and bandeiraea simplicifolia staining was see ... | 1985 | 3009360 |
| isolation of competition-defective mutants of rhizobium fredii. | we coupled tn5 mutagenesis with a competition assay to isolate mutants of rhizobium fredii usda 257 that are defective in competition for nodulation of soybeans. two mutants with single tn5 inserts in the chromosome showed reduced competitiveness in vermiculite but were identical to the wild-type strain in symbiotic properties when inoculated alone. recombination of tn5 and flanking genomic regions cloned from the mutants into the parent strain showed that tn5 was responsible for the mutant phen ... | 1987 | 3025184 |
| [rna-recognizing function of the dna-recognizing structure helix-turn-helix in ribosomal proteins s4 and s7 of escherichia coli and plant chloroplasts]. | the segments which satisfy the necessary requirements for dna-recognizing structure helix-turn-helix coding have been found in amino acid sequences of rna-recognizing e. coli ribosomal proteins s4 and s7. it has appeared that among these segments there are those that in chloroplasts s4 and s7 homologues satisfy these necessary requirements also: s4-97-121 and 108-132 (ec), 91-115 and 102-126 (zea mays); s7-109-133 (ec) 110-134 (euglena gracilis, glycine max). such segments have been confronted w ... | 1988 | 3054493 |
| mechanisms of neutrophil recruitment to the lung by grain dust exposure. | inhalation of grain dusts can cause symptoms of both acute and chronic bronchitis, which has been associated with a neutrophilic inflammatory response in the lung. since this influx of neutrophils may potentially result in bronchial injury, mechanisms of neutrophil recruitment to grain dust were examined. sterile, aqueous grain dust extracts were prepared from settled dusts of grain sorghum, corn, oats, and soybeans. the extracts were evaluated for their ability to directly attract human neutrop ... | 1988 | 3059885 |
| nickel as a micronutrient element for plants. | the detrimental effects of excessive ni on plant growth have been well known for many years. more recent evidence indicates that ni is required in small amounts for normal plant growth and development. ni is an essential component of urease in plants and microorganisms. a deficiency of ni in plants is reported to result in necrotic lesions in leaves in response to toxic accumulations of urea. urease plays an essential role in mobilization of nitrogenous compounds in plants, a process that is esp ... | 1988 | 3076427 |
| flavones and isoflavones as inducing substances of legume nodulation. | rhizobia are soil bacteria that can form symbiotic associations with leguminous plants leading to the fixation of atmospheric nitrogen to ammonia which the plant can use. this is an interaction which involves the exchange of many signals between the plant and the bacterium. to start this interaction, rhizobia have adapted to use flavonoid compounds, released by the plant root, as part of a regulatory system to initiate the transcription of their infection (nodulation, nod) genes. the development ... | 1988 | 3076431 |
| aflatoxin contamination in soybeans: role of proteinase inhibitors, zinc availability, and seed coat integrity. | soybean trypsin inhibitors are thought to ward off pathogens. studies with aflatoxigenic strains of aspergillus flavus and a. parasiticus, frequent soybean contaminants, revealed that trypsin inhibitors do not affect the growth of these fungi and aflatoxin production. further, the availability of zinc, an essential mineral for aflatoxin synthesis that was thought to explain increased aflatoxin accumulation in cooked compared with raw soybeans, was shown to decrease upon cooking. seed coat integr ... | 1986 | 3089160 |
| structure and evolution of the 4.5-5s ribosomal rna intergenic region from glycine max (soya bean). | the nucleotide sequence for the 4.5-5s ribosomal dna region from the chloroplastids of soya beans was determined as the basis of further comparative studies on the structure and evolution of this intergenic region. comparisons with other plant sequences as well as equivalent sequences in eubacteria suggest that the longer internal transcribed spacer regions of plants have evolved, at least in part, by dna sequence duplications and that the presence of the 4.5s rrna in chloroplast may result from ... | 1987 | 3116503 |
| effects of ca2+ on phytoalexin induction by fungal elicitor in soybean cells. | a glucan elicitor from the cell walls of the fungus phytophthora megasperma f.sp. glycinea caused increases in the activities of the phytoalexin biosynthetic enzymes, phenylalanine ammonia-lyase and chalcone synthase, and induced the production of the phytoalexin, glyceollin, in soybean (glycine max) cell suspension cultures when tested in culture medium containing 1.2 mmol/liter ca2+. removal of extracellular ca2+ by treatment with ethylene glycol bis(beta-aminoethyl ether)-n, n'-tetraacetic ac ... | 1987 | 3116938 |
| detection of surface carbohydrates on pneumocystis carinii by fluorescein-conjugated lectins. | lectins react with a wide range of different carbohydrates (table 1). even so-called monospecific anti-h(o) lectins from lotus tetragonolobus, ulex europaeus, and anguilla anguilla react not only with the anti-h determinant but also with several fucosylated carbohydrates. consequently, the type of lectin receptor existing on the surface of pneumocystis carinii should be determined, because only a carbohydrate analysis can demonstrate the kind of carbohydrates which exist on the cell surface of t ... | 1987 | 3125541 |
| ketones as electrophilic substrates of lipoxygenase. | the rate-limiting step of the lipoxygenase reaction involves the abstraction of a hydrogen from the methylene carbon of a 1,4-diene. one possibility for the mechanism of the enzyme is the abstraction of this hydrogen as a proton to generate a carbanionic intermediate or transition state. in order to investigate this possibility, 5-, 8-, 12-, and 15-hydroxy-eicosatetraenoic acid were oxidized to the corresponding ketones and these ketones were assayed as substrates of the 5-, 12-, and 15-lipoxyge ... | 1988 | 3135805 |
| lipoxygenase catalyzed oxygenation of hydroxy fatty acids to lipoxins. | the pure lipoxygenases from rabbit reticulocytes and soybeans convert a variety of substrates (arachidonic acid, 15-hpete, 15-hete, 5-hete, various dihete isomers) to trihydroxy eicosanoids containing a conjugated tetraene system (lipoxins). in general, the methyl esters are better substrates for lipoxin formation than are the free acids. lipoxygenase inhibitors (5,8,11,14-eicosatetraynoic acid, nordihydroguaiaretic acid) strongly inhibit the lipoxin formation. the complete stereochemistry of th ... | 1988 | 3138902 |
| [dynamics of respiration and the microbial biomass in soil supplemented with straw from corn and soybeans]. | soil organic matter level has an important influence on land productivity. this level depends upon the efficiency in partitioning carbon from plant debris into co2 and humified substances by microorganisms. the c/n ratio of the residues is one of the factors that affects this process. to study its influence on the growth and activity of the microflora, a soil from the corn region of buenos aires was supplemented with different amounts of straw from corn and soybean, and respiration and microbial ... | 1988 | 3140295 |
| effects of alpha-tocopherol, its carboxylic acid chromane compound and two novel antioxidant isoflavanones on prostaglandin h synthase activity and autodeactivation. | the natural antioxidant alpha-tocopherol has repeatedly been described to inhibit platelet aggregation and thromboxane formation, whereas its influence on prostaglandin h synthase in vivo and in vitro is a matter of controversy. in the present study the effects of different antioxidative compounds on ram vesicular gland microsomal prostaglandin h synthase activity were investigated in vitro: d,l-alpha-tocopherol, its carboxylic acid chromane compound (trolox), phytol, alpha-tocopherol-acetate an ... | 1988 | 3148864 |
| tracheobronchial epithelium of the sheep: iv. lectin histochemical characterization of secretory epithelial cells. | conventional histochemical characterization of the mucus secretory apparatus is often difficult to reconcile with the biochemical analysis of respiratory secretions. this study was designed to examine the secretory glycoconjugates in airways using lectins with biochemically defined affinities for main sugar residues of mucus. we used five biotinylated lectins--dba (dolichos biflorus) and sba (glycine max) for n-acetyl galactosamine (galnac), bsa i (bandeiraea simplicifolia) and pna (arachis hypo ... | 1988 | 3189886 |
| molybdate transport by bradyrhizobium japonicum bacteroids. | bacteroid suspensions of bradyrhizobium japonicum usda 136 isolated from soybeans grown in mo-deficient conditions were able to transport molybdate at a nearly constant rate for up to 1 min. the apparent km for molybdate was 0.1 microm, and the vmax was about 5 pmol/min per mg (dry weight) of bacteroid. supplementation of bacteroid suspensions with oxidizable carbon sources did not markedly increase molybdate uptake rates. anaerobically isolated bacteroids accumulated twice as much mo in 1 h as ... | 1988 | 3192511 |
| [experimental 3-month study of safety of textured soybeans becsei]. | the authors conducted a three-month safety test of a textured soy (ts) product manufactured by the agricultural plant, boly. young male and female rats received semisynthetic diets containing 8, 18 and 38% of ts protein or casein supplemented with methionine, as the only source of protein. the effect of the diet containing 18% of ts protein on the growth, on certain parameters of protein and fat metabolism and on the zinc and copper status corresponded to that produced by 18% of casein supplemen ... | 1988 | 3206854 |
| wheat germ splicing endonuclease is highly specific for plant pre-trnas. | intron-containing pre-trnas from organisms as different as yeast, nicotiana, xenopus and man are efficiently spliced and processed in a hela cell extract. they are also correctly processed in a wheat germ extract; however, the intron is removed only from the tobacco pre-trna. to determine whether plant pre-trna introns have any specific structural and/or sequence feature we have cloned two intron-containing trnatyr genes from the plant arabidopsis. comparison of these genes, of the nicotiana trn ... | 1988 | 3208752 |
| expression of glycoconjugates on normally developing and immunologically impaired hymenolepis diminuta. | the carbohydrates on the surface of hymenolepis diminuta were analyzed with gold-labelled lectins, and it was found that the surface coat of the anterior body differs from that of the strobila in its lectin-binding properties. binding sites for lectins from abrus precatorius (apa), arachis hypogaea (pna), glycine max (sba) and for wheat germ agglutinin (wga) and succinylated wga were located on the scolex and strobilation zone. lectin-gold particles attached mainly to the electron-dense spines. ... | 1988 | 3237676 |
| purification and characterization of locusta migratoria chymotrypsin. | a chymotrypsin-like enzyme (ctle) was isolated from the digestive tract of the african migratory locust locusta migratoria migratorioides by ion-exchange chromatography on diethylaminoethyl (deae) cellulose followed by affinity chromatography on phenylbutylamine (pba) sepharose. the purity and homogeneity of ctle have been shown by sds-page and on cellulose acetate strips. the enzyme has a molecular weight of 24,000, determined by sds-page and on a sephadex g-75 calibrated column. it has an isoe ... | 1988 | 3246483 |
| lectin histochemistry on mucous substances of the taste buds and adjacent epithelia of different vertebrates. | in the present study carbohydrate residues in taste buds (tbs) and adjacent epithelial formations of a teleostean fish, a frog and the rabbit were detected by means of lectin histochemistry. biotinylated lectins from pisum sativum (psa), arachis hypogaea (pna), dolichos biflorus (dba), triticum vulgaris (wga and succinylated wga), glycine max (sba) and ulex europaeus (uea i) have been applied. the lectins were bound to an avidin-biotin-peroxidase-complex (abc) and visualized by diaminobenzidine/ ... | 1988 | 3259218 |
| pyrene acts as a cocarcinogen with the carcinogens benzo[a]pyrene, beta-propiolactone and radiation in the induction of malignant transformation in cultured mouse fibroblasts; soybean extract containing the bowman-birk inhibitor acts as an anticarcinogen. | pyrene was found to act as a cocarcinogen in the induction of transformation of cultured balb/c3t3 cells by three different types of carcinogens: a direct acting chemical carcinogen, beta-propiolactone, a chemical carcinogen requiring metabolic activation, benzo[a]pyrene, and a physical carcinogen (60co) gamma radiation. since pyrene enhanced transformation in vitro by approximately the same amount for all the carcinogens tested, these results suggest that the carcinogenic action of pyrene is no ... | 1986 | 3267443 |
| carbohydrate-binding specificities of five lectins that bind to o-glycosyl-linked carbohydrate chains. quantitative analysis by frontal-affinity chromatography. | the carbohydrate-binding specificities of lectins purified from agaricus bisporus (aba-i), arachis hypogaea (pna), bauhinia purpurea (bpa), glycine max (sba), and vicia villosa (vva-b4) have been studied by affinity chromatography on columns of the immobilized lectins, and quantitatively analyzed by frontal affinity chromatography. these five lectins could be classified into two groups with respect to their reactivities with typical mucin-type glycopeptides, beta-d-galp-(1----3)-alpha-d-galpnac- ... | 1988 | 3274131 |
| high and low inhibitor soybean meals affect human duodenal proteinase activity differently: in vitro comparison of proteinase inhibition. | the purpose of the study was to investigate the in vitro inhibition of the proteolytic activity in samples of duodenal juice obtained during instillation of raw soybeans in humans. the results suggested the presence of an inhibitor-resistant trypsin. practically no inhibition was obtained with phenylmethylsulfonyl fluoride, aprotinin, human alpha 1-antitrypsin or lima bean trypsin inhibitor. specificity against synthetic substrates was different from that obtained in the absence of raw soybeans. ... | 1988 | 3282043 |
| distribution of k vitamins (phylloquinone and menaquinones) in human placenta and maternal and umbilical cord plasma. | it is a well-known fact that neonatal and infantile vitamin k deficiencies cause melena neonatorum and intracranial hemorrhagic disorders. these disorders occur more frequently in breast-fed babies than in bottle-fed babies, for reasons that are still obscure. endogenous concentrations of k vitamins (phylloquinone and menaquinones) in human placentas and in paired specimens of maternal and umbilical cord plasma were detected by high-performance liquid chromatography with fluorometric detection a ... | 1988 | 3348316 |
| proline metabolism in n2-fixing root nodules: energy transfer and regulation of purine synthesis. | n2-fixing root nodules of soybean (glycine max l. merr.) convert atmospheric n2 to ammonia(um) in an energy-intensive enzymatic reaction. these nodules synthesize large quantities of purines because nitrogen fixed by bacteria contained within this tissue is transferred to the shoots in the form of ureides, which are degradation products of purines. in animal systems, it has been proposed that proline biosynthesis by pyrroline-5-carboxylate reductase (p5cr) is used to generate the nadp+ required ... | 1988 | 3353366 |
| nutritive value of whole soybeans fermented with aspergillus oryzae or rhizopus oligosporus as evaluated by neonatal pigs. | two experiments were conducted to evaluate the nutritive effects of heated whole soybeans fermented with the mold aspergillus oryzae (aos) or rhizopus oligosporus (ros) in diets fed to neonatal pigs from 1 to 22 d of age. in experiment 1, either soybean meal or heated whole soybeans fermented with ros replaced 75% of the dried skim milk protein. the average daily gain (adg) and gain-feed (g-f) ratio of pigs fed the diet containing 100% milk protein were greater than those of pigs fed the diets c ... | 1988 | 3357058 |
| lactational response to soybean meal, heated soybean meal, and extruded soybeans with ruminally protected methionine. | seventy-three high producing holstein cows were arranged in a 3 x 2 factorial to evaluate three protein supplements (soybean meal, heat-treated soybean meal, and extruded blend of soybeans and soybean meal) without or with 15 g/head/d of ruminally protected dl-methionine during wk 4 through 16 postpartum. total mixed diets contained (dm basis) 30% corn silage, 15% alfalfa hay, and 55% of the respective concentrate mix. milk production was higher when cows were fed either heated soybean product i ... | 1988 | 3372801 |
| influence of jet-sploding and extrusion on ruminal and intestinal disappearance of canola and soybeans. | in situ rumen degradation and intestinal digestibility of dm and cp of jet-sploded and extruded protein sources were estimated, using two ruminally and duodenally cannulated lactating holstein cows, in two experiments. one-gram samples of ground (1 mm) whole canola seed, canola meal, extruded canola meal, jet-sploded whole canola seed, protec, soybean meal and extruded soybean meal (experiment 1), and whole canola seed jet-sploded at temperatures ranging from 116 to 177 degrees c (experiment 2) ... | 1988 | 3372817 |
| glycoconjugate expression in human renal oncocytomas. a lectin histochemical study. | a panel of lectins chosen for its specificity for segments of the nephron was used in an avidin-biotin-peroxidase reaction to stain 13 human renal oncocytomas and a specimen of oncocytomatosis. the lectins were derived from lotus tetragonolobus, glycine max, dolichos biflorus, and arachis hypogaea. two patterns predominated: one of staining with the lectins of d biflorus and g max, suggesting distal tubular or collecting-duct differentiation, and one of staining with the lectin of l tetragonolob ... | 1988 | 3395218 |
| high-affinity binding of fungal beta-glucan fragments to soybean (glycine max l.) microsomal fractions and protoplasts. | we have recently reported the existence of binding sites in soybean membranes for a beta-glucan fraction derived from the fungal pathogen phytophthora megasperma f. sp. glycinea, which may play a role in the elicitor-mediated phytoalexin response of this plant [schmidt, w. e. & ebel, j. (1987) proc. natl acad. sci. usa 84, 4117-4121]. the specificity of beta-glucan binding to soybean membranes has now been investigated using a variety of competing polyglucans and oligoglucans of fungal origin. p ... | 1988 | 3402458 |
| utilization by growing and finishing pigs of raw soybeans of low kunitz trypsin inhibitor content. | three trials were conducted to compare acceptance and utilization by growing and finishing pigs of diets containing supplemental protein from either heated, solvent-extracted soybean meal (sbm), raw low-kunitz trypsin inhibitor soybean (lt) or raw commercially grown williams cultivar soybean with high kunitz trypsin inhibitor content (ht). in trial 1, 36 crossbred pigs, averaging 7 kg in weight, were fed 1) corn-sbm, 2)corn-lt or 3) corn-ht diets for 28 d. diets were formulated to be isolysinic ... | 1988 | 3403398 |
| an evaluation of a dry-fat product as a source of supplemental energy in pig diets. | three feeding trials (278 pigs) and one balance trial (14 pigs) were conducted to evaluate acceptance and utilization of diets containing a dry-fat product containing 90% fat. the fat was a mixture of equal parts vegetable and animal fats. in trial 1, 4-wk-old pigs fed diets with 5% dry-fat added had average daily gain (adg), average daily feed intake (adfi) and gain/feed (g/f) values equal to pigs fed the diet containing 4.5% liquid fat, the unprocessed equivalent to the dry-fat. values for g/f ... | 1988 | 3403400 |
| carotenoid composition of serum and egg yolks of hens fed diets varying in carotenoid composition. | high performance liquid chromatography of yolks of hens fed a diet based on yellow corn, alfalfa, and soybeans revealed over 20 cartenoids. lutein, lutein monester, lutein diester, 3'-oxolutein, cryptoxanthin, zeaxanthin, beta-carotene, and zeacarotene were identified by their retention times, visible absorption spectra, behavior on saponification, and their presence or absence when lutein was the primary carotenoid fed. three weeks after placing the hens on a white corn-soy-based diet supplemen ... | 1988 | 3405937 |
| effect of soybean lipid on growth and ruminal nitrogen metabolism in cattle fed soybean meal or ground whole soybeans. | three experiments were conducted to determine the effect of soybean lipid on ruminal proteolysis of soybean meal (sbm) and ground whole soybeans (gsb). experiment 1 was a 92-d growth experiment using 120 calves (206 kg) allotted to 12 pens of 10 calves each. three replicate pens were assigned to each of the treatment supplements: low sbm (lsbm), low gsb (lgsb), high sbm (hsbm) and high gsb (hgsb). calves received ad libitum amounts of corn silage top-dressed with the respective supplement (.81 k ... | 1987 | 3443588 |
| ultrastructural studies of the liver and skeletal muscles of rats fed on soybeans. | | 1987 | 3448423 |
| influence of diets on growth in the rat. | the proportions of amino acids required for growth and maintenance are substantially different. alterations in nutrient profiles of soybeans due to hydrothermic alkaline processing have been reported. the purpose of the present work was to investigate the effects of amino acid losses during hydrothermic alkaline processing of soybeans on growth in the rat. the protein sources of diets a, b and c were untreated soybean cotyledones, hydrothermic alkaline processed soybeans and casein, respectively ... | 1987 | 3452459 |
| in vitro effect of soybean (glycine max l. merr)lectin on lipid peroxidation in pyridoxine deficient albino rats. | | 1987 | 3452602 |
| organization and characterization of genes essential for symbiotic nitrogen fixation from bradyrhizobium japonicum i110. | a total of 96 independent tn5 insertions within a 39-kilobase-pair (kbp) segment of chromosomal dna containing the three structural genes for nitrogenase (nifh, nifd, and nifk) from bradyhizobium japonicum i110 were obtained in escherichia coli and transferred to the wild-type strain by marker exchange. individual transconjugants containing a tn5 insertion were inoculated onto glycine max cv. wilkin (soybeans) and analyzed for their effect on symbiotic nitrogen fixation. in addition to the three ... | 1986 | 3462181 |
| variability in molybdenum uptake activity in bradyrhizobium japonicum strains. | twenty naturally occurring strains of bradyrhizobium japonicum in 11 serogroups were screened for the ability to take up mo as bacteroids from soybean root nodules. the strains varied greatly in their ability to take up mo in a 1-min period. the best strain was usda 136, which had an mo uptake activity of almost 3.0 pmol/min per mg of bacteroid (dry weight). in contrast, the poorest strain, usda 62, had an mo uptake activity of 0.35 pmol of mo per min per mg of bacteroid. there were similarities ... | 1987 | 3473064 |
| lectin binding sites on human endocervix: a comparison with secretory and proliferative endometrium. | endocervix and corresponding endometrium of women of reproductive age were studied histochemically with 13 fluorescein isothiocyanate-labeled lectins to delineate the differences between the epithelial cells in two anatomical sites. lectin from maclura pomifera (mpa), ulex europaeus (uea-i), glycine max (sba), and vicia villosa (vva) bound only to endocervical epithelium and were the only four lectins that distinguished endocervical from endometrial epithelium. these differences were independent ... | 1986 | 3526979 |
| endogenous lectins from cultured soybean cells: isolation of a protein immunologically cross-reactive with seed soybean agglutinin and analysis of its role in binding of rhizobium japonicum. | incubation of rhizobium japonicum with the cultured soybean cell line sb-1, originally derived from the roots of glycine max, resulted in specific adhesion of the bacteria to the plant cells. this binding interaction appears to be mediated via carbohydrate recognition, since galactose can inhibit the heterotypic adhesion but glucose cannot. affinity chromatography, on a sepharose column derivatized with n-caproyl-galactosamine, of the supernatant fraction of a sb-1 cell suspension after enzymati ... | 1986 | 3528167 |
| lectin binding patterns in developing rat colon. | to evaluate qualitative changes in brush-border and goblet cell glycoconjugates during colonic development, rhodamine-conjugated lectins were applied to tissue from fetal (18-22 day), suckling (1-20 day), and weanling (22-26 day) rats. tissue from the proximal and distal colon of each animal was incubated with triticum vulgaris, arachis hypogaea, glycine max, or ulex europeus agglutinin i. formalin-fixed paraffin sections and unfixed frozen sections were coded, read blindly, and graded from nega ... | 1987 | 3557008 |
| protected methionine supplementation with extruded blend of soybeans and soybean meal for dairy cows. | methionine may be the first amino acid limiting milk production in early lactation cows. to evaluate this further, 23 high producing holstein cows (9 multiparous and 14 primiparous) were fed an extruded blend of soybeans and soybean meal (40:60) without or with 15 g of added dl-methionine as 50 g of ruminally protected methionine product during wk 4 to 16 postpartum. cows were fed a 15.8% crude protein total mixed ration consisting of 30% (dry basis) corn silage, 15% alfalfa hay, and 55% concent ... | 1987 | 3571638 |
| aberrant, canavanyl protein formation and the ability to tolerate or utilize l-canavanine. | l-canavanine, 2-amino-4-(guanidinooxy)butyric acid, and l-arginine incorporation into de novo synthesized proteins was compared in six organisms. utilizing l-[guanidinooxy14c]canavanine and l-[guanidino14c]arginine at substrate saturation, the canavanine to arginine incorporation ratio was determined in de novo synthesized proteins. caryedes brasiliensis and sternechus tuberculatus, canavanine utilizing insects; canavalia ensiformis, a canavanine storing plant; and to a lesser extent heliothis v ... | 1987 | 3582574 |
| a locus encoding host range is linked to the common nodulation genes of bradyrhizobium japonicum. | by using cloned rhizobium meliloti, rhizobium leguminosarum, and rhizobium sp. strain mpik3030 nodulation (nod) genes as hybridization probes, homologous regions were detected in the slow-growing soybean symbiont bradyrhizobium japonicum usda 110. these regions were found to cluster within a 25-kilobase (kb) region. specific nod probes from r. meliloti were used to identify noda-, nodb-, nodc-, and nodd-like sequences clustered on two adjacent hindiii restriction fragments of 3.9 and 5.6 kb. a 7 ... | 1987 | 3584066 |
| endogenous lectin from cultured soybean cells. chemical characterization of the lectin of sb-1 cells. | a lectin has been identified in the cell line, sb-1, originally derived from the roots of glycine max. this lectin, which we shall refer to as sb-1 lectin, was isolated on the basis of its carbohydrate-binding activity (affinity chromatography on sepharose column derivatized with n-caproyl-galactosamine) and its immunological cross-reactivity (immunoblotting with rabbit antibodies directed against seed soybean agglutinin (sba]. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) ... | 1987 | 3584143 |
| amino acid sequence of a soybean (glycine max) seed polypeptide having a poly(l-aspartic acid) structure. | a polypeptide of mr 4400 was isolated from soybean (glycine max) seeds by extraction with 60% ethanol followed by ion-exchange and reverse-phase chromatography. the peptide contains an unusually high amount of aspartic acid residues (approximately 25%, and hence is designated as soybean aspartic acid-rich peptide). its complete primary structure was determined by conventional methods to be the following. ser-lys-trp-gln-his-gln-gln-asp-ser-cys-arg-lys-gln-leu-gln-gly-val-asn- leu-thr-pro-cys-glu ... | 1987 | 3611081 |
| histochemical characterization of glycoproteins present in jejunal and colonic goblet cells of pigs on different diets. a biopsy study using chemical methods and peroxidase-labelled lectins. | we examined the glycoprotein composition of intestinal goblet cells in jejunal and colonic biopsies obtained from pigs on different diets. paraffin sections were stained both chemically and with the following horseradish-peroxidase conjugated lectins: canavalia ensiformis (con-a), limulus polyphemus (lpa), lotus tetragonolobus (lta), arachis hypogaea (pna), ricinus communis (rca1), glycine max (sba) and triticum vulgaris (wga). using chemical staining procedures, only small quantitative differen ... | 1987 | 3624000 |
| a mechanism for the hypocholesterolaemic activity of saponins. | 1. saponins are steroid or triterpene glycosides which occur in a number of important food plants, including such staples as soya beans (glycine max) and chickpeas (cicer arietinum). they are known to be hypocholesterolaemic. 2. some saponins form an insoluble complex with cholesterol which prevents its absorption from the small intestine. others cause an increase in the faecal excretion of bile acids, an indirect route for elimination of cholesterol. 3. we have investigated the effects of diffe ... | 1986 | 3676181 |
| high-mr glycoprotein profiles in human milk serum and fat-globule membrane. | gradient-polyacrylamide-gel electrophoresis of human milk serum separated three high-mr glycoprotein bands. the properties of the components corresponding to the three bands (tentatively termed 'components c, b and a' in their order of migration) were compared by staining with four monoclonal antibodies and lectins. components b and c both reacted with the four antibodies, but component a did not. components b and c were stained with peanut (arachis hypogaea) agglutinin (pna) and wheat (triticum ... | 1986 | 3707520 |
| cell surface carbohydrate of leishmania mexicana amazonensis: differences between infective and non-infective forms. | the cell surface carbohydrates of leishmania mexicana amazonensis (amastigotes and promastigotes, both infective and non-infective forms) were comparatively analyzed by agglutination assay employing 28 highly purified lectins, and by binding assay using 125i-labeled lectins. among the d-galnac binding lectins, bandeiraea simplicifolia-i, dolichos biflorus, phaseolus vulgaris and glycine max were highly specific for the amastigotes, while that from maclura aurantiaca selectively agglutinated prom ... | 1986 | 3709546 |
| secretory glycoproteins of major vestibular glands (bartholin's glands) in female calves. studies with usual histochemical methods and with lectin-horseradish peroxidase conjugates in normal animals and in animals implanted with anabolic drugs. | the nature of mucins synthetized by the major vestibular glands of normal female calves and animals treated by anabolic drugs was investigated by usual histochemical methods and by lectin conjugate methods: canavalia ensiformis (con a), limulus polyphemus (lpa), lotus tetragonolobus (lta), arachis hypogaea (pna), ricinus communis (rca i), glycine max (sba) and triticum vulgaris (wga). two main secretory cell types, i.e. acinar and tubular cells, could be distinguished. the former produced sialom ... | 1986 | 3751491 |
| a comparison of lectin binding in rat and human peripheral nerve. | eleven different fluorescein- or peroxidase-conjugated lectins with different sugar-binding affinities were employed to analyze and compare glycoconjugates of rat and human peripheral nerves at the light microscopic level. a majority of lectins showed a distinct binding pattern in different structures of the nerve. lectin binding was similar but not identical in rat and human nerves. limulus polyhemus agglutinin did not stain any structures in rat or human nerves. in both species, all other lect ... | 1986 | 3772078 |
| molecular cloning and expression of rhizobium fredii usda 193 nodulation genes: extension of host range for nodulation. | dna hybridization with the cloned nodulation region of rhizobium meliloti as a probe revealed dna homology with four hindiii fragments, 12.5, 6.8, 5.2, and 0.3 kilobases (kb) in size, of the symbiotic plasmid prjausda193. both hybridization and complementation studies suggest that the common nodulation genes nodabc and nodd of r. fredii usda 193 are present on the 5.2-kb hindiii and 2.8-kb ecori fragments, respectively, of the sym plasmid. both fragments together could confer nodulation ability ... | 1986 | 3782034 |
| genetics and breeding of soybeans lacking the kunitz trypsin inhibitor. | the major trypsin inhibitor present in soybean [glycine max (l.) merrill] seed is the kunitz trypsin inhibitor or soybean trypsin inhibitor a2 (sbti-a2). four forms of sbti-a2 have been identified in the u.s. soybean germplasm collection. three of the forms designated ti(a), ti(b), and ti(c) are electrophoretically distinguishable from one another by their different rf values of 0.79, 0.75, and 0.83, respectively. the three forms are inherited as codominant alleles in a multiple allelic series a ... | 1986 | 3799281 |
| relevance of multiple soybean trypsin inhibitor forms to nutritional quality. | trypsin inhibitors contribute to the antinutritional component of raw soybean meal by inhibiting vertebrate pancreatic serine proteinases in the small intestine, resulting in a range of deleterious physiological effects in the animal. the variation in the nutritional quality of soybean cultivars stems partly from wide-ranging levels of trypsin inhibitor, and from varying proportions of trypsin inhibitors of two classes--the kunitz and the bowman-birk inhibitor classes. the latter class is better ... | 1986 | 3799285 |
| immunological evidence for gap junction polypeptide in plant cells. | a whole cell homogenate prepared from soybean (glycine max (l.) merr. cv. mandarin) root cells (sb-1 cell line) was electrophoresed on a sodium dodecyl sulfate-polyacrylamide gel and transferred to nitrocellulose paper. the nitrocellulose was probed with a monospecific antibody capable of recognizing the mr 27,000 polypeptide of rat liver gap junctions; this antibody was prepared from immune serum raised against gap junctions purified from v79 cells (chinese lung fibroblasts). the immunoblots af ... | 1987 | 3805027 |
| soybean hydrophobic protein. isolation, partial characterization and the complete primary structure. | a 9000-mr protein isolated from a 60% ethanolic extract of soybean (glycine max) seeds has been characterized and fully sequenced. the protein consists of 80 amino acid residues with four disulfide bonds. it contains a large number of hydrophobic residues and lacks methionine, phenylalanine, tryptophan, lysine and histidine residues. the protein readily crystallizes from water but is quite soluble in aqueous organic solvents like 95% 1-propanol. it aggregates to form large molecules (above 80 kd ... | 1987 | 3830151 |
| elicitor-induced phytoalexin synthesis in soybean cells: changes in the activity of chalcone synthase mrna and the total population of translatable mrna. | rapid changes in the mrna activity encoding chalcone synthase, a central enzyme involved in isoflavonoid phytoalexin synthesis, were induced in cultured cells of soybean (glycine max) after treatment with a glucan elicitor from the cell walls of the fungus, phytophthora megasperma f. sp. glycinea, a soybean pathogen. two-dimensional gel electrophoresis of the in vitro- and in vivo-synthesized chalcone synthase showed that it consisted of a group of proteins of similar molecular weights of about ... | 1985 | 3855251 |
| the bowman-birk inhibitor. trypsin- and chymotrypsin-inhibitor from soybeans. | four decades of studies on the isolation, characterization, properties, structure, function and possible uses of the bowman-birk trypsin- and chymotrypsin-inhibitor from soybeans are reviewed. starting from bowman's acetone insoluble factor, designated ai, aa and sbtiaa, the bowman-birk inhibitor (bbi) was found to be a protein molecule consisting of a chain of 71 amino acids cross linked by 7 disulfide bonds, with a tendency to self-associate. bbi possesses two independent sites of inhibition, ... | 1985 | 3886572 |
| the complete amino acid sequence of trypsin inhibitor de-3 from erythrina latissima seeds. | trypsin inhibitor de-3 from erythrina latissima seeds contains 172 amino acids, including 4 half-cystine residues, and resembles the kunitz-type inhibitors. limited hydrolysis of de-3 with trypsin at ph 3 produced two fragments, f1 and f2, containing 63 and 109 amino acids, respectively. amino-terminal sequence studies revealed that f1 was the n-terminal and that f2 was the c-terminal fragment. the complete amino acid sequence of fragments f1 and f2 was then determined on peptides produced by en ... | 1985 | 3902809 |
| an immunofluorescent analysis of lectin binding to normal and regenerating skeletal muscle of rat. | ten different fluorescein-conjugated lectins of various known sugar specificities were used to study cell surface glycoconjugates of normal and regenerating rat skeletal muscle. in normal muscle, canavalia ensiformis agglutinin, triticum vulgaris agglutinin (wheat germ agglutinin, wga), ricinus communis agglutinin-i, and maclura pomifera agglutinin bound strongly to the endomysial region of the myofibers. no binding was observed in the cytoplasm of the myofibers. other lectins (dolichos biflorus ... | 1985 | 3915936 |
| lectin characterization of gonococci from an outbreak caused by penicillin-resistant neisseria gonorrhoeae. | a total of 40 neisseria gonorrhoeae isolates, representing 19 penicillin-resistant isolates (from 8 heterosexual patients and 11 homosexual patients) and 21 penicillin-susceptible isolates (from 15 heterosexual patients and 6 homosexual patients) and obtained from the same geographic area, were examined. lectin agglutination patterns were based on the reactivity of the isolates with the following 14 lectins: concanavalin a, lens culinaris, trichosanthes kinlowii, griffonia simplicifolia i, arach ... | 1985 | 3935658 |
| aflatoxin-induced alterations in glycine max, cv. 'essex' root cell membrane protein content. | aflatoxins (afts) are hepatocarcinogens, mutagens, teratogens and toxins. isolates of afts-producing strains of aspergillus flavus can grow upon both autoclaved soybeans and to a lesser extent field-grown beans. besides inhibiting the elongation of excised, in vitro cultured soybean roots, the afts can impair the roots' ability to remove [14c]-leucine from a culture medium and to incorporate the amino acid into acid-insoluble, cytoplasmic protein. in this connection, exogenous afts once taken-up ... | 1985 | 3935929 |
| enzymatic properties of the lipoxygenase from pea seeds. | a lipoxygenase preparation was obtained from dried green pea seeds. disc electrophoresis with enzyme staining indicated the presence of only one main isoenzyme corresponding to the isoenzyme pl i according to yoon and klein (j. agric. food chem. 24, 955 (1979)), whereas pl ii was absent. the assay for pea lipoxygenase has been optimized by using a final concentration of 0.53 mm potassium linoleate in the presence or absence of 0.2% sodium cholate. without detergent the rho h optimum was 5.9, in ... | 1985 | 3936491 |
| race:cultivar-specific induction of enzymes related to phytoalexin biosynthesis in soybean roots following infection with phytophthora megasperma f. sp. glycinea. | primary roots of soybean [glycine max (l.), cv harosoy 63] seedlings were inoculated with zoospores from either race 1 (incompatible, host resistant) or race 3 (compatible, host susceptible) of phytophthora megasperma f. sp. glycinea (pmg) and the activities of phenylalanine ammonia-lyase (pal), chalcone synthase (chs), isoflavone synthase, and dihydroxypterocarpan 6a-hydroxylase related to phytoalexin (glyceollin) biosynthesis, and of glucose-6-phosphate dehydrogenase (glc-6-pdh) and glutamate ... | 1986 | 3963819 |
| isolation and characterization of a soybean lectin having 4-o-methylglucuronic acid specificity. | a new lectin from soybeans having specificity toward the extracellular 4-o-methyl-d-glucurono-l-rhamnans produced by certain strains of rhizobium japonicum has been purified and characterized. isolation was accomplished initially by isoelectric precipitation of contaminating globulins and subsequently by affinity chromatography on partially hydrolyzed glucuronorhamnan covalently coupled to amino-hexylagarose. residual globulins were removed by adsorption of the lectin on concanavalin a-agarose a ... | 1986 | 3964668 |
| legume agglutinins that bind to rhizobium meliloti. | a protein found in seeds and roots of alfalfa (medicago sativa) was implicated in the specificity of the infection process, based on its binding to the symbiont rhizobium meliloti. we found an agglutinin with similar properties in seeds and roots of sweet clover (melilotis alba). the sweet clover differed from alfalfa in nodulation by a mutant strain of r. meliloti, but the agglutinins were indistinguishable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, rhizobium agglutination, a ... | 1985 | 3988714 |
| assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism. | the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ... | 1985 | 4016573 |
| raw soybeans for gestating swine. | an experiment was conducted to determine the influence of feeding ground raw soybeans to swine during gestation on reproductive performance for three consecutive parities. sixty crossbred gilts were bred and randomly assigned to two dietary treatments with 30 gilts/treatment. the diets were formulated to contain 14% crude protein with either soybean meal (sbm) or raw soybeans (rsb) serving as the source of supplemental protein. all animals were fed in individual gestation stalls and were given 1 ... | 1985 | 4038700 |
| physiological and pathobiological significance of ocular glycoproteins. i. studies using fluorescein labelled glycine max. | cell surface carbohydrates play an important role in several biological, immunological, and neoplastic phenomena including development, growth regulation, cellular locomotion, receptor activation, and tumour metastasis. fluorescein labelled lectins which bind to specific carbohydrate residues in glycoproteins and glycolipids are being increasingly used as chemical probes to study cell components. several different preparations of ocular tissues from human, rabbit, and rat were examined for the d ... | 1985 | 4038883 |
| protein degradation in rumen and amino acid absorption in small intestine of lactating dairy cattle fed heat-treated whole soybeans. | four lactating holstein cows fitted with rumen cannulae and t-type cannulae in proximal duodenum and terminal ileum were used to measure protein degradation in the rumen and amino acid flow and absorption in the small intestine. soybean meal, whole soybeans, and whole soybeans extruded at 132 and 149 degrees c provided 50% of the protein in diets that contained 51% grain, 36% corn silage, and 13% alfalfa hay (dry matter). spot samples of digesta were collected from duodenum and ileum during 96 h ... | 1985 | 4038985 |
| protection against dimethylhydrazine-induced adenomatous tumors of the mouse colon by the dietary addition of an extract of soybeans containing the bowman-birk protease inhibitor. | dimethylhydrazine (dmh) induces adenocarcinomas of the colon and rectum in rodents; this animal cancer model system is thought to closely resemble human colorectal cancer. an extract of soybeans containing the bowman-birk protease inhibitor added to the diet of dmh-treated mice resulted in a significant suppression of adenomatous tumors of the colonic mucosa. non-treated mice maintained on the same diet for most of their natural life-span (1.5 years) showed no observable health problems. | 1985 | 4040444 |