| lectin histochemistry of 1,2-dimethylhydrazine-induced rat colon neoplasia. | lectins linked to fluorescein were used as carbohydrate probes to examine the goblet cell mucin and epithelial cell surface glycoconjugate alterations in an experimental rodent model of colonic neoplasia induced with parenteral 1,2-dimethylhydrazine dihydrochloride. lectins derived from triticum vulgare (wga), ricinus communis (rca1), and limulus polyphemus (lpa) showed reduced labeling of goblet cell mucin in these tumors, while binding with peanut lectin from arachis hypogaea (pna), a lectin o ... | 1983 | 6688437 |
| lectins as markers of human epidermal cell differentiation. | the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ... | 1982 | 6816653 |
| binding of fitc-coupled peanut-agglutinin (fitc-pna) to embryonic chicken retinas reveals developmental spatio-temporal patterns. | fitc-labeled peanut agglutinin (pna), a lectin from peanut (arachis hypogaea) which binds specifically to d-galactose, was used as a probe to trace the distribution of pna-lectin receptors during early development of chick retina. we found that specific fluorescence within the inner plexiform layer (ipl) and the outer plexiform layer (opl) appears at different developmental stages. the specific fluorescence of the ipl first appears near the central area of the ventro-posterior quadrant of the 7- ... | 1983 | 6871724 |
| production of bacillus sphaericus strain 1593 primary powder on media made from locally obtainable nigerian agricultural products. | five media, formulated from dried cow blood, mineral salts, and seeds from four species of legumes, were assessed for growth, sporulation, and insecticidal properties of bacillus sphaericus strain 1593. bacterial powders, prepared from broth, were assayed against culex quinquefasciatus, anopheles gambiae, and aedes aegypti. good growth and sporulation were obtained with all the media. the highest number of viable cells and spores per mililitre (8.6 x 10(8) and 8.1 x 10(8] were obtained in media ... | 1983 | 6883226 |
| alterations in human colonic mucin occurring with cellular differentiation and malignant transformation. | the binding of fluorescein isothiocyanate (fitc)-conjugated lectins to mucin in the human colon was studied by using fluorescence microscopy. in normal mucosa, lectins that preferentially bind to exposed n-acetyl-galactosamine residues (dolichos biflorus agglutinin and soybean agglutinin) bound selectively to the goblet cell mucin of well-differentiated cells in the upper colonic crypt. by contrast, lectins that require exposed non-reducing galactose residues for binding (ricinus communis agglut ... | 1982 | 6952252 |
| the major sialoglycoprotein of human t-lymphocytes. | human, peripheral-blood t-lymphocytes and human, t-lymphoblastoid cells of a molt 4b cell-line were surface-labeled by lactoperoxidase-catalyzed iodination, periodate and sodium borotritide, and galactose oxidase and sodium borotritide, and analyzed by dodecyl sodium sulfate-polyacrylamide gel-electrophoresis. both types of cells were found to show a major, cell-surface sialoglycoprotein with an apparent mol. wt. of 95,000. after neuraminidase treatment, this glycoprotein showed a higher mol. wt ... | 1980 | 6965465 |
| lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins. | in cellular immunology, peanut (arachis hypogaea) lectin has been used to selectively agglutinate immature lymphoid cells and soybean (glycine max-lectin to agglutinate b lymphocytes. we have used affinity chromatography to study the surface glycoproteins of rat and mouse lymphoid cells which bind to these lectins. thymocyte and t and b lymphocyte glycoproteins were analysed either without modification (native) or after the removal of sialic acid with neuraminidase (asialo). the only native glyc ... | 1982 | 6980823 |
| the role of lectins in blood group serology. | many lectins display blood group activity, and extracts from dolichos biflorus (anti-a1), ulex europaeus (anti-h), and vicia graminea (anti-n) seeds provide an alternative to human sera as a source of blood-typing reagents. however, the major application of lectins in blood group serology undoubtedly lies in the recognition and elucidation of red celll polyagglutination. in this respect, lectins from arachis hypogaea (anti-t/tk), salvia sclarea (anti-tn). salvia horminum (anti-tn + cad). dolicho ... | 1980 | 6998652 |
| extent of desialation of blood group mm, nn, and mn antigens required for reactivity with human anti-t antibody and arachis hypogaea lectin. | immunochemical activity of the arachis hypogaea lectin has been equated, in spite of its different hapten combining requirements, with that of the human anti-t (thomsen-friedenreich) antibody population which is of importance in cancer immunology. we show here by gradual desialation of isolated human blood group mm and nn antigens at ph 2.0, 56 degrees c, that a. hypogaea lectin is, in addition, substantially more tolerant to neuac in the vicinity of t antigen's immunodominant repeating structur ... | 1982 | 7061447 |
| purification and characterization of protease inhibitors from peanuts (arachis hypogaea). | five protease inhibitors were isolated from peanut seeds and named a-i, a-ii, b-i, b-ii, and b-iii. these inhibitors seemed to be bowman-birk type inhibitors judging from their low molecular weights and high cystine contents. all the inhibitors inhibited both bovine trypsin and chymotrypsin at ratios of 1:2 and 1:1, respectively, but not simultaneously. the complexes of the inhibitors and trypsin no longer inhibit chymotrypsin. on the other hand, their complexes with chymotrypsin inhibit trypsin ... | 1982 | 7096296 |
| circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols. | the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ... | 1982 | 7150429 |
| the surface glycoproteins of human skin fibroblasts detected after electrophoresis by the binding of peanut (arachis hypogaea) agglutinin and ricinus communis (castor-bean) agglutinin i. | a new methodology was developed to study the cell-surface glycoproteins of cultured human skin fibroblasts. this was based on the binding of a variety of biotinyl-lectins to nitrocellulose electrophoretic transfers of total fibroblast lysates after separation in sodium dodecyl sulphate/polyacrylamide gels, followed by reaction with avidin-biotinyl-peroxidase complexes and detection with 3,3'-diaminobenzidine. the technique proved to be very sensitive and a large number of glycoproteins were dete ... | 1982 | 7159403 |
| interaction of lectins from soybean and peanut with rhizobia that nodulate soybean, peanut, or both plants. | four of 14 strains of rhizobium japonicum from soybean nodulated peanut (arachis hypogaea l. cultivar jumbo virginia), and 3 of 8 rhizobium sp. strains from peanut nodulate soybean (glycine max (l.) merr. cultivar harosoy 63). cells of three peanut rhizobia bound fluorescent- and radioisotope-labeled soybean lectin. two of these strains failed to nodulate soybean, and conversely, two peanut strains that nodulated soybean did not bind to soybean lectin. both culture medium and age had pronounced ... | 1980 | 7195297 |
| simple test for detection of virus neuraminidase and antineuraminidase using lectins (lectin-neuraminidase test system). | the fast and sensitive detection of virus neuraminidase effectiveness (influenza virus a and b or mumps virus) and of antibodies against virus neuraminidase in human serum is described. lectin mainly obtained from arachis hypogaea and helix pomatia is used in the lectin-neuraminidase test (ln-test). the lectins are capable of agglutinating erythrocytes after virus incubation because virus neuraminidase reveals the t-antigen to be situated on erythrocytes. the presence of anti-neuraminidase in hu ... | 1980 | 7223125 |
| [neuraminidase-producing pneumococci in the pathogenesis of hemolytic-uremic syndrome]. | hemolytic-uremic syndrome (hus) accompanied by pneumococcal infections forms a characteristic subgroup of hus. pneumococcal neuraminidase splits off neuraminic acid from the glycoproteins present on the surface of red cells, thrombocytes and endothelial cells, and thus exposes the hidden thomsen cryptantigen (t-ag). the t-ag can then react with a complement-fixing antibody of the igm class which is present in all human plasmas after the age of 6 months. early diagnosis of t-transformation should ... | 1980 | 7280595 |
| [experiments to elucidate the role of aspergillus fungi in the hemorrhagic syndrome in poultry]. | attempts were made to assess the role played by fungi of the aspergillus genus in the occurrence of the hemorrhagic syndrome in birds. groups of test birds were given continuously a forage mixture containing 30 per cent of ground nut cake which had aflatoxin at the rate of 1.5 ppm, a forage mixture 30 per cent of which was preliminarily contaminated with spores of asp. fumigatus or asp. flavus, and subtoxic amounts of nonpurified aflatoxin. growth retardation was established along with the devel ... | 1981 | 7331224 |
| peanut lectin binding properties of germinal centres of mouse lymphoid tissue. | peanut (arachis hypogaea) lectin (pnl) has been shown to agglutinate the 90% of cells from murine thymus which are supposed to be immature cortical thymocytes. further studies on the numbers of thymocytes binding fluorescein isothiocyanate conjugated pnl (fitc-pnl) confirmed the large proportion of pnl binding cells. in other organs such as bone marrow, spleen and peripheral lymph nodes, smaller proportions of pnl positive cells have been recorded. pnl-positive cells outside the thymus have been ... | 1980 | 7360273 |
| evidence of accumulation of ceramides containing [14c]nervonic acid in the rat lung following injection of [14c]erucic acid. | a mixture of albumin-bound [14c]erucate and [3h]oleate was injected into rats fed a stock pellet diet containing 4% by weight of lipid. chylomicrons containing the same labelled fatty acids were also injected into rats fed diets containing 15% by weight of rapeseed oil (48% of erucic acid), canbra oil (less than 5% of erucic acid) or ground nut oil (no erucic acid). lung lipids were analyzed at various times after injection. in all cases, except in the rapeseed oil diet group, 14c radioactivity ... | 1980 | 7370286 |
| preneoplastic and neoplastic lesions of rat hereditary renal cell tumors express markers of proximal and distal nephron. | long-evans (eker) rats carry a mutation that predisposes them to develop spontaneous renal cell tumors of two morphologic patterns: solid chromophilic masses or cystic lesions lined by eosinophilic cells. previous studies have suggested that these tumors arise from the proximal tubules. in the present study, lectin-binding characteristics and cytokeratin expression of various stages of hereditary rat renal epithelial neoplasia were examined to localize the portion of the nephron from which tumor ... | 1995 | 7483212 |
| intermediate filament expression and lectin histochemical features of canine transmissible venereal tumour. | immunocharacterization of intermediate filament proteins and lectin-binding studies were carried out in canine transmissible venereal tumour (tvt), a unique neoplasia sharing some epidemiological features with kaposi's sarcoma in humans. neoplastic cells express vimentin, but neither cytokeratin nor desmin. regarding lectins, tvt cells express receptors for triticum vulgaris (wga), concanavalia ensiformis (con a) and ricinus communis i (rca-i). they appear to be negative for ulex europaeus-i (ue ... | 1995 | 7488385 |
| echinostoma caproni and e. trivolvis alter the binding of glycoconjugates in the intestinal mucosa of c3h mice as determined by lectin histochemistry. | mouse (c3h) mucosal glycoconjugates were examined in normal small intestines and intestines infected with echinostoma caproni or e. trivolvis using six different fluorescein-conjugated lectins: triticum vulgaris agglutinin (wga), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin i (rca-i), glycine max soybean agglutinin (sba), dolichos biflorus agglutinin (dba), and arachis hypogaea peanut agglutinin (pna). the expression of lectin-binding sites and the intensity of the binding of ... | 1993 | 7507137 |
| porcine mitogen-induced interferon. | induction of porcine interferon (poifn) with different lectins (phytohaemagglutinin, lens culinaris lectin, arachis hypogea lectin) in various concentrations (500, 50 and 5 micrograms/ml) was studied. kinetics of poifn appearance was analyzed by antiviral and antiproliferative tests in human transformed and nontransformed cells. the relation between antiviral and antiproliferative activity of poifn in terms of respective units was determined. poifn was characterized in comparison with human inte ... | 1994 | 7526670 |
| lectin binding distinguishes between neuroendocrine and neuronal derivatives of the sympathoadrenal neural crest. | lectin cytochemistry was used to identify surface epitopes selectively expressed by chromaffin cell chemoreceptors (glomus cells) in the rat carotid body. unexpectedly, these studies revealed that binding sites for peanut agglutinin (pna; arachis hypogea) were highly expressed by all neuroendocrine-derivatives of the sympathoadrenal neural crest, including glomus cells, small, intensely fluorescent cells, and adrenal chromaffin cells in situ. in contrast, principal sympathetic neurons did not ex ... | 1995 | 7535839 |
| genetic and phenetic analyses of bradyrhizobium strains nodulating peanut (arachis hypogaea l.) roots. | seventeen bradyrhizobium sp. strains and one azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16s rrna gene (rdna), (ii) randomly amplified dna polymorphisms (rapd) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv) utilization of 21 aliphatic and 22 aromatic substrates, and (v) intrinsic resistances to seven antibiotics. partial 16s rdna analysis revealed the presence of only two rdna homolog ... | 1995 | 7538280 |
| localization of peanut (arachis hypogaea) root lectin (pra ii) on root surface and its biological significance. | the glucose-specific peanut root lectin, pra ii, is localized on the surface of 7-day-old peanut seedling root and in root cortical parenchymatous cells. the lectin is eluted from intact roots upon washing with buffer containing glucose. rabbit erythrocytes bind to the root surface and the cortical cells; the binding is inhibited by antibodies raised against pra ii, peanut-specific rhizobium cells and by glucose. lipopolysaccharides isolated from host-specific rhizobium strain inhibit the haemag ... | 1995 | 7540901 |
| [h2o2 generation and human neutrophil aggregation as affected by lectins]. | the effects of eight plant lectins on human neutrophils aggregation and h2o2 release were studied. both processes were stimulated by lectins from viscum album, triticum vulgaris, phaseolus vulgaris and canavalia ensiformis while lectins from solanum tuberosum, sambucus nigra and glycine mas displayed only aggregating activity and arachis hypogaea lectin was not effective. heating (46 degrees c) and uv-radiation during 4-5 min were found to suppress completely h2o2 release from neutrophils keepin ... | 1995 | 7557235 |
| glycoconjugate expression of chondrocytes and perichondrium during hyaline cartilage development in the rat. | alterations in the expression of glycoconjugate structures during cartilage development in the chondrocranium, nasal skeleton, meckel's cartilage, limb buds, vertebral bodies and ribs were investigated comparatively in 13 to 21-d-old rat embryos. the binding patterns of 24 biotinylated lectins were analysed in serial sections and compared with results obtained using histochemical methods. proteoglycan distribution, assessed by conventional staining procedures, was not associated with lectin bind ... | 1995 | 7591987 |
| effect of dietary fat on diethylnitrosamine induced hepatocarcinogenesis in wistar rats. | in a diethylnitrosamine (den) induced hepatoma model, the effect of fish oil, a rich source of eicosapentaenoic acid (epa) and docosahexaenoic acid (dha), and ground nut oil, a rich source of oleic acid (oa) and linoleic acid (la), was studied. both 20% fish oil and ground nut oil inhibited den-induced hepatocarcinogenesis in rats. contrary to general belief, an inverse correlation was observed between the development of neoplastic nodules and the amount of lipid peroxides formed in the high fat ... | 1995 | 7656238 |
| laboratory evaluation of chemical control of aflatoxin production in unshelled peanuts (arachis hypogaea l.). | propionic acid (ammonium salt) at 3000 mg/kg (pa1) and 5000 mg/kg (pa2) of unshelled peanuts (up); grapefruit seed extract at 5000 mg/kg (gf1) and 10,000 mg/kg (gf2); sodium orthophenylphenate at 2500 mg/kg (sop1) and 5000 mg/kg (sop2); thiabendazole 1000 mg/kg (tbz1) and 5000 mg/kg (tbz2) were studied in the laboratory, to verify their efficiency in controlling fungal growth and aflatoxin (af) production on moist up (16-18% moisture content). moist up were put into polyethylene bags with cotton ... | 1995 | 7664926 |
| idiotypes of pre-existing human anti-carcinoma anti-t and anti-tn antibodies. | all humans normally possess antibodies, predominantly igm, that react specifically with the thomsen-friedenreich (t) and the tn antigens which are present in immunoreactive form on > 85% of all human carcinomas, but not in healthy and otherwise diseased tissues. we report here a serological study of idiotype expression and antigen reactivity of the anti-t and anti-tn antibodies. idiotypy was analyzed with rabbit antibodies raised against, and made specific for, affinity-purified polyclonal anti- ... | 1993 | 7680895 |
| determination of the sialic acid linkage specificity of sialidases using lectins in a solid phase assay. | a procedure for the determination of activity and linkage specificity of sialidases is described. the sialoglycoprotein fetuin is coated onto a microtiter plate and incubated with sialidases from different sources. enzymatic activities and linkage specificities are then determined by a sandwich method which measured the binding of different lectins to fetuin. the lectins used were peanut agglutinin (pna) from arachis hypogaea, which binds specifically the galactose beta-1-3-n-acetylgalactosamine ... | 1993 | 7686353 |
| relationship between ricinus communis agglutinin-1 binding and nucleolar organizer regions in human gliomas. | histochemical staining using lectins from ricinus communis (rca-1), arachis hypogaea, and canavalia ensiformis was investigated in 40 human gliomas, three central neurocytomas, one human neuroblastoma cell line (imr-32), and two normal brain tissues. staining was uniform in low-grade gliomas, but heterogeneous in high-grade gliomas, particularly with rca-1. the correlation between rca-1 reactivity and cellular proliferative potential was investigated in 10 high-grade gliomas using a combined sta ... | 1993 | 7689179 |
| characterization of glycoconjugate expression during development of meckel's cartilage in the rat. | the staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. a ubiquitous distribution of binding sites was demonstrated after incubation with con a (canavalia ensiformis), dsl (datura stramonium; except bone matrix), and wga (triticum vulgare). ecl (erythrina cristagalli), gsl i (griffonia simplicifolia), sja (saphora japonica), vvl (vicia villosa), dba (dolichus biflorus), uea ... | 1995 | 7717533 |
| changes in glycan distribution within the porcine interhaemal barrier during gestation. | changing patterns of glycan distribution are described in porcine placentae at 15, 19, 26, 43, 58, 69 and 109 days gestation, using a carefully selected panel of lectins that allowed partial analysis of saccharide classes and sequences. the lectins used were from galanthus nivalis, pisum sativum, phaseolus vulgaris (leukohaemagglutinin), triticum vulgaris, tetragonolobus purpureus, ulex europaeus-1, arachis hypogaea, erythrina cristagalli, glycine max, maclura pomifera, wisteria floribunda, doli ... | 1995 | 7736552 |
| [lectin-binding proteins of plasma membranes of a human trophoblast]. | spectra of lectin-binding proteins of the human trophoblast were studied by the method affinity blotting using a set of loctins conjugated with peroxidase. the set included lectins lens culinaris (lcl), canavalia ensiformis (con a), ricinus communis (rca 1), arachis hypogaea (pna), triticum vulgaris (wga), laburnum anagyroides (lal). presence of n- and o-glycans in the composition of proteins of plasmatic membranes of a trophoblast was shown, partial characteristics of their oligosugar chains be ... | 1994 | 7747337 |
| histochemical study of lectin binding in the human fetal minor salivary glands. | the emerging synthesis of glycoconjugates containing specific oligosaccharides in developing human fetal labial and lingual salivary glands has been investigated by lectin histochemistry. an avidin-biotin technique was used to study the binding of lectins from ulex europeus i (uea-i), dolichos biflorus (dba), glycine maximus (sba), helix pomatia (hpa), arachis hypogaea (pna) and triticum vulgare (wga) to specific sugars on sections of tissue from labial glands, glands of blandin and nuhn, glands ... | 1995 | 7776265 |
| presence of vicia graminea lectin- or vicia unijuga lectin-binding (vgu) glycoproteins, vgu glycoproteins with thomsen-friedenreich (t) activity and t-active glycoproteins in human meconium. | perchloric acid-soluble fraction prepared from a mixture of meconiums of 22 newborn babies was subjected to a systematic affinity chromatography using vicia unijuga lectin-cellulofine column and arachis hypogaea lectin-agarose column and separated into four fractions, vgu glycoprotein, vgu glycoprotein with t activity, t-active glycoprotein and another glycoprotein fractions. hplc analysis showed that the first fraction contained three glycoproteins with molecular weight (mw) of about 19,400 kda ... | 1995 | 7780836 |
| use of lectins to evaluate the effects of gaas softlaser on dog tendon. | 1. lectins labeled with colloidal gold particles were used for the ultrastructural evaluation of the biological effects of gaas softlaser irradiation on the healing of dog tendon wounds. 2. six dogs were submitted to tenotomy and tenorrhaphy on the right and left hind legs. all animals received laser irradiation (4 j/cm2) daily for ten days only on the left leg, and the right leg of the same animal was used as control. biopsies were taken 11, 22 and 40 days after surgery. 3. laser-irradiated and ... | 1994 | 7787808 |
| putative glycoprotein and glycolipid polymorphonuclear leukocyte receptors for the actinomyces naeslundii wvu45 fimbrial lectin. | recognition of receptors on sialidase-treated polymorphonuclear leukocytes (pmns) by the gal/galnac lectin associated with the type 2 fimbriae of certain strains of actinomyces results in activation of the pmns, phagocytosis, and destruction of the bacteria. in the present study, plant lectins were utilized as probes to identify putative pmn receptors for the actinomyces lectin. the gal-reactive lectin from ricinus communis (rcai), the gal/galnac-reactive lectins from r. communis (rcaii) and bau ... | 1995 | 7790078 |
| a sheep hydatid cyst glycoprotein as receptors for three toxic lectins, as well as abrus precatorius and ricinus communis agglutinins. | the binding properties of a glycoprotein with blood group p1 specificity isolated from sheep hydatid cyst fluid with gal and galnac specific lectins was investigated by quantitative precipitin and precipitin inhibition assays. the glycoprotein completely precipitated ricinus communis agglutinin (rca1), abrus precatorius agglutinin (apa) and mistletoe toxic lectin-i (ml-i). only 1.0 microgram of p1 glycoprotein was required to precipitate 50% of 5.1 micrograms ml-i nitrogen. it also reacted well ... | 1995 | 7827100 |
| recognition of a cd4+ mouse medullary thymocyte subpopulation by amaranthus leucocarpus lectin. | we have used the gal beta(1-->3)galnac-specific amaranthus leucocarpus lectin to isolate a thymus cell subpopulation which is different from that sorted with arachis hypogaea lectin. the cells recognized by a. leucocarpus lectin were predominantly cd4+, whereas a minor proportion of cd8+ cells (approximately 11%) were also identified. the a. leucocarpus-positive cells were located in the thymus medulla and the cortico-medullary junction. the cortex was negative for a. leucocarpus cells. | 1994 | 7835965 |
| lectin-histochemical analysis of glycans in ovine and bovine near-term placental binucleate cells. | chorionic binucleate cells (bnc) occur in several ruminants including cow, deer, goat and sheep. they migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. we have compared the bnc of near-term, resin-embedded, ovine and bovine placentae using 15 biotinylated lectins and an avidinperoxidase revealing system. there was pronounced conservation of saccharides between the two species. several sub-types of n-gl ... | 1994 | 7850870 |
| tubular atrophy in the end-stage kidney: a lectin and immunohistochemical study. | atrophic tubules in end-stage renal disease (esrd) may have various morphologic appearances: some show microscopic features of "classic" atrophic tubules (thick, wrinkled tubular basement membrane and simplified epithelium), others show "thyroidization" (round tubules with simplified epithelium and casts), and many have the appearance of "endocrine" tubules (small tubules with narrow lumina, clear cells, and relatively thin basement membranes). other tubules in esrd may be enlarged and dilated w ... | 1994 | 7906246 |
| in situ characterization of the human hyalocyte. | to provide more definitive evidence regarding the cellular origin of hyalocytes and to establish a basis for their identification in tissue specimens. | 1994 | 7945040 |
| differential lectin binding of onchocerca lienalis and onchocerca ochengi infective larvae following their development in simulium ornatum s.l. | lectins have been used to investigate species specific differences in carbohydrate moieties on the surface of the infective larvae of two onchocerca species following their development in simulium ornatum. of the seven fitc-labelled lectins used in this study only two, arachis hypogea (pna) and helix pomatia (hpa), bound to the surface of o. lienalis, whereas no lectin binding could be detected on the surface of o. ochengi infective larvae. this indicates that in principal lectins might be a pot ... | 1994 | 8066375 |
| segmental differentiation of permeability, protein glycosylation, and morphology of cultured bovine lung vascular endothelium. | the barrier function, surface biochemistry, and morphology of confluent monolayers of endothelial cells isolated from different segments of the bovine lung vasculature [microvessels (blmvec), vein (bpvec) and artery (bpaec)] were grown in culture and compared. a number of common cell surface proteins were identified along with two proteins of 46 and 48 kda found exclusively on bpvec. lectin affinity chromatography revealed multiple glycosylation differences. the lectins, arachis hypogaea (aha) a ... | 1994 | 8123001 |
| characterization of a calcium-dependent protein kinase from arachis hypogea (groundnut) seeds. | a calcium-dependent protein serine/threonine kinase (gncdpk) has been detected in groundnut (arachis hypogea) seeds that specifically phosphorylates a peptide (mlcpep) representing the phosphate-accepting domain of smooth muscle myosin light chains. gncdpk has been purified to near homogeneity from the soluble fraction of groundnut seeds by ammonium sulfate precipitation, q sepharose, blue sepharose, and sephacryl 300 chromatography. the molecular weight of gncdpk is estimated to be 53,000. enzy ... | 1994 | 8165262 |
| production of extracellular acidic lipase by rhizopus arrhizus as a function of culture conditions. | thermophilic strain of rhizopus arrhizus accumulates an acidic lipase in culture fluid when grown in a medium containing ground nut oil, milk powder and inorganic salts. addition of 2.0% ground nut oil yielded the highest productivity of enzyme. soyabean meal and arabinose were found to be the best nitrogen and carbon sources for enzyme production respectively. addition of metal ions such as mncl2, sncl2 and cacl2 increased the enzyme productivity by 4 fold. the enzyme productivity in the fermen ... | 1993 | 8181953 |
| lectins as markers of rumen epithelial cell differentiation. | lectins of different carbohydrate specificities (gna (galanthus nivalis), con a (canavalia ensiformis), vfl (vicia faba), psl (pisum sativum), lca (lens culinaris), pna (arachis hypogaea; with or without prior neuraminidase treatment), wga (triticum vulgare), sba (glycine max), uea-i (ulex europaeus), lpa (limulus polyphemus), bs-i b4 (bandeiraea simplicifolia, isolectin b4)) were explored for use as differentiation markers of rumen epithelial cells in vivo and in vitro. lectins specific for man ... | 1994 | 8206789 |
| schistosoma mansoni: surface membrane isolation with lectin-coated beads. | lectins from lens culinaris and arachis hypogaea immobilized on polyacrylamide beads were used for selective isolation of glycosylated surface membrane domains of adult schistosoma mansoni worms, and the method was compared with the membrane isolation procedure developed with polycationic (affi-gel) beads. the lentil lectin proved to be suitable for interaction with surface membrane components: an increment in the specific activities of tegumental phosphohydrolases was observed in the bound frac ... | 1993 | 8231898 |
| immunohistochemical and lectin dissection of the human nephron in health and disease. | many renal diseases involving the tubular epithelium appear to preferentially affect certain nephron segments. while major portions of the nephron, such as proximal and distal convoluted tubules and collecting ducts, can be identified in the normal kidney, the distinction of diseased nephron segments can be difficult in tissue sections. thus, to identify which nephron segments are involved in pathologic changes is usually impossible by routine histologic examination alone. recently antibody and ... | 1993 | 8250694 |
| crystallographic refinement of bowman-birk type protease inhibitor a-ii from peanut (arachis hypogaea) at 2.3 a resolution. | the crystal structure of bowman-birk type protease inhibitor a-ii from peanut was refined at 2.3 a resolution using a restrained least-squares method. the crystallographic r-factor is 0.196 for 7697 reflections with f > 3 sigma (f) in the range from 6.0 to 2.3 a resolution. two molecules in an asymmetric unit are independently refined and, their structures are compared with each other. the inhibitor molecule has an elongated shape with two reactive sites, one at both ends of the longest dimensio ... | 1993 | 8254669 |
| analysis of the ability of three probes targeting the outer acrosomal membrane or acrosomal contents to detect the acrosome reaction in human spermatozoa. | a comparative analysis has been undertaken of the behaviour of probes targeting the outer acrosomal membrane (arachis hypogaea lectin) or constituents of the acrosomal vesicle (pisum sativum lectin or monoclonal antibody crb9) following the induction of the acrosome reaction in human spermatozoa with the ionophore a23187. the results obtained with these two classes of reagent were highly correlated (r = approximately 0.8), although the absolute rates of acrosome reaction were significantly diffe ... | 1993 | 8300825 |
| lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. lectin binding pattern of normal peripheral nerve in man. | the binding patterns of lectins to normal peripheral nerves were examined. twelve biotinylated lectins were used in this study; canavalia ensiformis (con a), pisum sativum (psa), lens culinaris (lca), ricinus communis 1 (rca-1), arachis hypogaea (pna), glycine max (sba), sophora japonica (sja), bandeiraea simplicifolia 1 (bsl-1), triticum vulgaris (wga), succinylated wga (s-wga), ulex europaeus 1 (uea-1) and helix pomatia (hpa). cytoplasm of schwann cells and perineurial cells was stained by con ... | 1993 | 8310810 |
| changes of lectin staining pattern of the golgi stack during differentiation of the ameloblast in developing rat molar tooth germs. | changes of lectin staining patterns in the golgi stack during cell differentiation were examined in the ameloblasts of developing rat molar tooth germs, using hrp-labeled lectins: canavalia ensiformis (con a), griffonia simplicifolia i (gs-i), glycine max (sba), ulex europeus i (uea-i), triticum vulgaris (wga), and arachis hypogaea (pna). the golgi stacks of the inner enamel epithelial cells and the presecretory ameloblasts were stained with the lectins, although the staining strength and patter ... | 1993 | 8338238 |
| effect of hypoxia on endothelial cell surface glycoprotein expression: modulation of glycoprotein iiia and other specific surface glycoproteins. | exposure to hypoxia alters many aspects of endothelial cell metabolism and function; however, changes in surface glycoconjugates under these conditions have not been extensively evaluated. in the current studies, we examined surface glycoproteins of cultured bovine aortic (baec) and pulmonary arterial (bpaec) endothelial cells under standard culture conditions (21% oxygen) and following exposure to hypoxia (0% oxygen) for varying time periods (30 min to 18 h) using a system of biotinylation, lec ... | 1993 | 8375475 |
| differential distribution of glycoconjugates in human reproductive tract. | to study the distribution of glycoconjugates in the epithelia of various regions of the human female reproductive tract and to examine whether lectins can be used as specific probes to define cell populations in the human female reproductive tract. | 1993 | 8419223 |
| disease resistance results from foreign phytoalexin expression in a novel plant. | although phytoalexins have long been inferred to be important in the defence of plants against fungal infection, there are few reports showing that they provide resistance to infection. several plants, including grapevine, synthesize the stilbene-type phytoalexin resveratrol when attacked by pathogens. stilbenes with fungicidal potential are formed in several unrelated plant species, such as peanut (arachis hypogaea), grapevine (vitis vinifera) and pine (pinus sylvestris). stilbene biosynthesis ... | 1993 | 8421520 |
| changes in the lectin binding sites on the testicular, epididymal, vas, and ejaculated spermatozoon surface of dog. | changes in the localization of sperm surface glycocomponents of testicular, epididymal, vas deferens, and ejaculated spermatozoa of dog (canis domesticus) were studied employing fluorescein isothiocyanate conjugated lectins viz., concanavalin a (cona), triticum vulgaris (wga), maclura pomifera (mpa), and arachis hypogaea (pna) agglutinins. the plasma membrane clothing the acrosome of the testicular, epididymal, and vas deferens spermatozoa shows reactivity with all the lectins used. however, in ... | 1993 | 8427417 |
| plasma membrane alterations of maturing goat (capra indicus) spermatozoa: lectin-binding and freeze-fracture study. | a qualitative and quantitative analysis of lectin-binding sites has been undertaken on spermatozoa recovered from different regions of the epididymis of the goat (capra indicus) using fluorescein isothiocyanate-linked lectins (bauhinia purpurea bpa, concanavalin a con a, dolichos biflorus dba, maclura pomifera mpa, arachis hypogaea or peanut agglutinin pna, glycine max or soyabean agglutinin sba, ulex europaeus uea, and triticum vulgaris or wheat-germ agglutinin wga), in conjunction with scannin ... | 1993 | 8443831 |
| distribution of lectin binding sites in the oviducts of cycling and hormone-treated pigs. | gamete transport, fertilization, and early embryonic development take place in different regions of the oviduct and under different hormonal conditions. the objective of this study was to use lectins to detect variation in the distribution of glycosylated molecules on the surface of the epithelia that influence these events. oviducts were collected from gilts on day 1 (estrus) and day 16 (diestrus) of the estrous cycle, and from gilts that were bilaterally ovariectomized on day 4 of the estrous ... | 1993 | 8487017 |
| photoreceptor degeneration and altered distribution of interphotoreceptor matrix proteoglycans in the mucopolysaccharidosis vii mouse. | recent studies suggest that chondroitin sulfate proteoglycans in the retinal interphotoreceptor matrix (ipm) play a role in maintaining photoreceptor viability. we report herein studies on the retinas from mice with mucopolysaccharidosis type vii (mps vii), a storage disorder resulting from virtual absence of beta-glucuronidase, an enzyme that is involved in the lysosomal degradation of chondroitin sulfate and other beta-glucuronide-containing proteoglycans. the distribution of ipm chondroitin s ... | 1993 | 8500564 |
| improvement of the traditional method of ogiri production and identification of the micro-organisms associated with the fermentation process. | fermented products were developed from different proportions of melon (citrullus vulgaris schrad) and groundnut (arachis hypogea) seeds after a 96 hour fermentation period. proximate analysis, carried out on both fermented and unfermented samples, indicated that only the total carbohydrate content was appreciably reduced by the fermentation process. micro-organisms responsible for fermentation were identified by gram staining and lactophenol staining, as bacteria and fungi. identified bacteria w ... | 1993 | 8506243 |
| capacitated and acrosome reacted spermatozoa of goat (capra indicus): a fluorescence and electron microscopic study. | plasma membrane alterations accompanying in vitro capacitation and acrosome reaction of goat spermatozoa were studied using lectin labelling, scanning electron microscopy, and freeze-fracture methods. fluorescein isothiocyanate linked lectins namely; canavalia ensiformis (cona), maclura pomifera (mpa), arachis hypogaea (pna), glycine max (sba) and triticum vulgaris (wga) agglutinin were used to examine the distribution of surface carbohydrates during these two events. the head and the sperm tail ... | 1993 | 8517552 |
| neurons with perineuronal sulfated proteoglycans in the human visual cortex, with special reference to their reactions to lectins. | the human visual cortex, especially its ganglionic lamina, was found to contain many neurons with perineuronal sulfated proteoglycans which were stained with cationic iron colloid and aldehyde fuchsin. it also contained many neurons with surface glycoproteins labeled with lectin vicia villosa agglutinin (vva) or glycine max agglutinin (sba). double staining frequently showed that the neurons stained with cationic iron colloid were not labeled with lectin vva or sba. hyaluronidase and chondroitin ... | 1995 | 8527242 |
| carbohydrate and peptide antigens in macrophage populations derived from human bone marrow and milk: an immunomorphological and immunochemical analysis. | an immunomorphological and immunochemical study was performed to elucidate the pattern of carbohydrate antigens and their relationships to the cluster differentiation (cd) 68 epitopes on macrophages derived from human bone marrow and milk. core and backbone antigens recognized by lectins from bauhinia purpurea (bpa), helix pomatia (hpa), arachis hypogaea (pna), glycine max. (sba), griffonia simplicifolia (gsa-i-b4), lycopersicon esculentum (lea) and erythrina cristagalli (eca) were expressed by ... | 1995 | 8550384 |
| lectin binding to extracellularly melanized microfilariae of brugia malayi from the hemocoel of anopheles quadrimaculatus. | binding patterns of fluorescein isothiocyanate (fitc)- and gold-conjugated lectins to extracellularly melanized sheathed and exsheathed microfilariae of subperiodic brugia malayi, isolated from and in situ in the abdominal hemocoel of anopheles quadrimaculatus 72-hr postinfection, were examined. five fitc-conjugated lectins [helix pomatia agglutinin (hpa), arachis hypogaea (peanut agglutinin-pna), triticum vulgaris (wheat germ agglutinin-wga), lens culinaris (lentil-lch), and concanavalin a (con ... | 1995 | 8568282 |
| characterization of two class ii chitinase genes from peanut and expression studies in transgenic tobacco plants. | two different genes encoding class ii chitinases from peanut (arachis hypogaea l. cv. nc4), a.h.chi2;1 and a.h.chi2;2, have been cloned. in peanut cell suspension cultures, mrna levels of a.h.chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from botrytis cinerea. the second gene, a.h.chi2;1, was only expressed after treatment with the fungal spores. transgenic tobacco plants containing the complete peanut a.h.chi2;1 gene exhibited essentially the same expres ... | 1996 | 8616259 |
| differential in vitro and in vivo behavior of three strains of trypanosoma cruzi in the gut and hemolymph of rhodnius prolixus. | a comparison was made of the agglutination and lysis of three strains of trypanosoma cruzi in gut extracts and hemolymph of rhodnius prolixus and the results obtained were correlated with the success or failure of the parasite strain to infect the digestive tube or to survive in the hemocel after inoculation. both t. cruzi strains dm28c and cl urine 35 days after feeding with parasites. concomitantly, both of these strains were agglutinated but not lysed by the crop extracts. in contrast, t. cru ... | 1996 | 8617337 |
| fluorescence-labeled fucolectins are superior markers for flow cytometric quantitation of the human sperm acrosome reaction. | to evaluate the binding of three fluorescein (fitc)-labeled fucose-specific lectins, anguilla anguilla agglutinin (aaa), tetragonolobus purpureas agglutinin (tpa), and ulex europaeus-1 agglutinin (uea-1), to unfixed, acrosome-intact and acrosome-reacted (ar) human sperm by flow cytometry and to compare the results with those found using fitc-labeled pisum sativum agglutinin (psa) and arachis hypogaea agglutinin (pna). | 1996 | 8654649 |
| lectin histochemistry of pregnant rat uterine tissues. | glycoconjugate residues were examined in the rat uterus at days 10, 12 and 15 of pregnancy using 17 biotinylated lectins with specificities for a variety of carbohydrate moieties. a wide variety of glycoconjugate residues were detected in the cytoplasm of some antimesometrial and mesometrial decidual cells with lectins from canavalia ensiformis (con a), lens culinaris (lch) culinaris (lch), pisum sativum (psa), griffonia simplicifolia ii (gs-ii), triticum vulgaris (wga), griffonia simplicifolia ... | 1996 | 8655407 |
| increase in lectin binding sites on epithelial cells by chronic bladder infection in rats. | using lectin histochemistry we assessed whether chronic bladder infection modifies carbohydrate residues of glycoconjugates on uroepithelial cells in rats. the bladder infection was produced by implanting a knotted silk thread with escherichia coli into the bladder. one or 4 weeks after the implantation the bladder was excised, incubated with sixteen biotinylated lectins and stained. the bladder epithelia as a whole stained more strongly positive for eight lectins in the infected rats than in th ... | 1996 | 8659017 |
| a histological, lectin and s-100 histochemical study of the developing prenatal human sublingual salivary gland. | lectin and s-100 protein histochemistry during fetal growth and development (10-38th gestational weeks) of these glands was studied. the histological development of glandular structures followed the known pattern for other salivary glands. using biotinylated lectins ulex europeus-i, dolichos biflorus, glycine maximus (soyabean), helix pomatia, arachis hypogaea (peanut) and triticum vulgare (wheatgerm), the binding level and, by implication, the concentration of associated specific oligosaccharid ... | 1995 | 8670026 |
| binding properties of a blood group le(a+) active sialoglycoprotein, purified from human ovarian cyst, with applied lectins. | studies on the structures and binding properties of the glycoproteins, purified from human ovarian cyst fluids, will aid the understanding of the carbohydrate alterations occurring during the biosynthesis of blood group antigens and neoplasm formation. these glycoproteins can also serve as important biological materials to study blood group a, b, h, le(a), le(b), le(x), le(y), t and tn determinants, precursor type i and ii sequences and cold agglutinin i and i epitopes. in this study, the bindin ... | 1996 | 8672551 |
| characterization of the mucins produced by normal human colonocytes in primary culture. | mature goblet cells filled with mucin ready for secretion represent about one third of the cells in primary cultures of human colonocytes. in the present study characterization of the mucins produced by cultured human colonocytes was made by histochemical methods by lectin and monoclonal antibody binding. two monoclonal antibodies and three lectins (dolichos biflorus (dba), helix pomatia (hpa) and arachis hypogea (pna) recognizing epitopes or sugar haptens characteristic of different stages of m ... | 1996 | 8739831 |
| lectin-binding sites on human sperm during acrosome reaction: modifications judged by electron microscopy/flow cytometry. | biochemical surface modifications occur during the capacitation and acrosome reaction of human sperm and among those, variations in the expression of carbohydrates moieties. a sequential study was performed with electronic microscopy and flow cytometry techniques, where the binding of 4 lectins was assessed on normal human sperm samples during in vitro induction of the acrosome reaction with calcium ionophore a-23187. triticum vulgaris agglutinin (wga) was shown to bind strongly the whole surfac ... | 1996 | 8743350 |
| cellular heterogeneity in the membrana granulosa of developing rat follicles: assessment by flow cytometry and lectin binding. | the hormone-mediated maturation of ovarian follicles is apparently accompanied by position-specific differentiation of cells of the membrana granulosa. we have assessed the extent of this cellular heterogeneity by flow cytometry using a variety of fluorescein isothiocyanate-labeled lectins as probes. follicular development was stimulated in immature rats by treatment with either diethylstilbestrol (des) or equine cg (ecg). lectin binding to monodispersed rat granulosa cells was then analyzed by ... | 1996 | 8770935 |
| aging of the human endometrium: peri-implantation phase endometrium does not show any age-dependent variation in lectin binding. | to evaluate if the peri-implantation endometrium shows age variations in lectin patterns, which suggest possible age variations in embryo-maternal recognition. | 1996 | 8801134 |
| sperm capacitation and the acrosome reaction are compromised in teratospermic domestic cats. | the efficiency of sperm capacitation and of the acrosome reaction was studied in the teratospermic domestic cat to evaluate further the etiology of compromised zona pellucida penetration and oocyte fertilization. specific objectives were to compare normospermic and teratospermic cat ejaculates for 1) the kinetics and timing of sperm capacitation in vitro as determined by an ionophore-induced acrosome reaction; 2) the incidence of spontaneous acrosomal loss; 3) the ability of capacitated, swim-up ... | 1996 | 8835386 |
| lectin binding to renaut bodies. | localization of sugar residues of renaut bodies in human sural nerves was studied using lectin histochemistry. lens culinaris specific to mannose and triticum vulgaris specific to glcnac bound to the capsular and core portions of renaut bodies strongly. arachis hypogaea specific to galb(1-3)galnac and ulex europaeus i specific to l-fucose bound to renaut bodies granularly. lectins specific to galactose or terminal galnac bound to nowhere. the perineurium showed similar lectin binding to renaut b ... | 1995 | 8848942 |
| changes in en(a-) human red blood cell membranes during in vivo ageing. | the human red blood cells with phenotype en(a-) were characterized by the lack of mn antigens. the red blood cells with phenotype en(a-) which were found in a japanese family were tested to clarify the changes in membrane surfaces of the red blood cells during in vivo ageing. the contents of sialic acid, glucose, mannose, galactose, fucose, n-acetylglucosamine and n-acetylgalactosamine of the red blood cell membranes obtained from the old red blood cells with phenotype en(a-) were significantly ... | 1996 | 8866734 |
| differential reactivities of the arachis hypogaea (peanut) and vicia villosa b4 lectins with human ovarian carcinoma cells, grown either in vitro or in vivo xenograft model. | pna and vva b4 recognize the tumor-associated t antigen and its immediate precursor tn, respectively. we found that both lectins are highly reactive in vitro, with human ovarian carcinoma cell lines, but only vva b4 bound significantly to breast and oral cancer cells. this binding is inhibited by specific monosaccharides. the lectin binding receptors were purified, revealing a glycoprotein of 32 kda for pna, and two glycoproteins of 35 and 38 kda for vva b4. in vivo localization of pna was almos ... | 1996 | 8898074 |
| glycoproteins in human parotid saliva assessed by lectin probes after resolution by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. | human parotid salivary glycoproteins separated by gradient sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and electroblotted onto nitrocellulose have been investigated using a battery of biotinylated lectin probes of characterized sugar specificity. lectin binding, detected on blots using avidin-biotin complex (abc) and a chemiluminescence generating substrate, was recorded on photographic film and compared with the original fluorescein isothiocyanate (fitc) stained blots ... | 1996 | 8907524 |
| the satellite rnas associated with the groundnut rosette disease complex and pea enation mosaic virus: sequence similarities and ability of each other's helper virus to support their replication. | pea enation mosaic virus (pemv) and the causal agents of groundnut rosette disease are diverse examples of disease complexes involving two rna species, one of which is related to the genomes of luteoviruses and the other to those of umbraviruses. in both complexes, these viral rna components may be supplemented with satellite rnas that are dependent on the umbravirus component for replication and systemic movement, and on the luteovirus component for encapsidation and vector transmission. sequen ... | 1996 | 8922480 |
| lectins and antibodies against blood-group antigens as tools for studying the cellular source of glycoproteins in human bronchial fluid: a comparison of morphological and biochemical observations. | we used nine lectins and three antibodies directed against abo blood-group antigens in morphological and western-blot experiments to investigate the source of secretory products of human large airways. in tissue sections, the lectins from griffonia simplicifolia (type i b4), dolichos biflorus, and helix pomatia, and the antibodies to the a, b, and/or h-antigen bound to mucous gland cells and to goblet cells; the binding of these substances was dependent on secretor status and abo blood group. th ... | 1996 | 8929348 |
| lectin histochemistry of the submandibular and sublingual salivary glands in rats. | tissue sections from rat submandibular and sublingual glands were studied with lectin probes to identify terminal sugars of the glycoconjugates in various cell types of the salivary glands. the lectins used in the study were canavalia ensiformis (con a), triticum vulgaris (wga), succinyl wga (s-wga) ricinus communis i (rca-i), arachis hypogaea (pna), and ulex europeaus (uea-i). the cytoplasm and cell membrane of both the serous and mucous acinar cells present high similarity in the distribution ... | 1996 | 8930643 |
| distribution of lectin binding sites in human bone marrow. identification by use of an ultrastructural postembedding technique. | the purpose of this ultrastructural study was to detect various carbohydrate residues on mature elements of the major human haematopoietic cell lines (granulopoiesis, erythropoiesis and megakaryopoiesis), sinus endothelium and plasma cells under comparable experimental conditions. marrow specimens were processed according to a modified postembedding technique with unicryl as embedding resin. a broad panel of 10 digoxigenin (dig)-conjugated lectins was applied for staining and specificity was eva ... | 1996 | 8933737 |
| genetic diversity among bradyrhizobium isolates that effectively nodulate peanut (arachis hypogaea). | symbiotic gene diversity and other measures of genetic diversity were examined in bradyrhizobium isolates that form an effective symbiosis with peanut (arachis hypogaea). initially, restriction fragment length polymorphism (rflp) analysis using a nitrogenase (nif) gene probe was performed on 33 isolates along with one bradyrhizobium elkanii and two bradyrhizobium japonicum strains. considerable diversity was observed among the rflp patterns of many of the isolates, especially those from south am ... | 1996 | 8941988 |
| differential histochemical peanut agglutinin stain in benign and malignant human prostate tumors: relationship with prostatic specific antigen immunostain and nuclear dna content. | the histochemical binding pattern of the peanut (arachis hypogaea) lectin (pna) was quantitatively described by means of computer-assisted microscope analysis in 28 benign prostatic hyperplasias (bph), 15 prostatic intraepithelial neoplasias (pin), and 119 prostatic adenocarcinomas. pna exhibits nonimmune but selective binding to glycoproteins with beta-d-galactosyl(1,3)-n-acetyl-d-galactosamine residues. we also investigated whether a relationship existed between the number of histochemical-rel ... | 1996 | 8958309 |
| induction of acrosome reaction in human spermatozoa accelerates the time of pronucleus formation of hamster oocytes after intracytoplasmic sperm injection. | to assess the relationship between the incidence of acrosome reaction (ar) and the timing of pronucleus (pn) formation after intracytoplasmic sperm injection (icsi). | 1997 | 9022609 |
| immune responses following cocktails of inactivated measles vaccine and arachis hypogaea l. (groundnut) or cocos nucifera l. (coconut) oils adjuvant. | haemagglutination inhibition (hai), geometric mean titre (gmt) and lymphocyte transformation test (ltt) were measured following inoculation of erythrocebus patas with inactivated measles vaccine (imv) and oils of arachis hypogaea l (go), cocos nucifera l (co), freund's complete and incomplete adjuvants (fca, fia). the gmt of hai were: go + imv = 8.50 +/- 4.15: co + imv = 8.08 +/- 4.03; fca + imv = 7.67 +/- 4.08. fia + imv = 7.08 +/- 4.08 compared with controls of live measles vaccine (lmv) = 7.3 ... | 1996 | 9032902 |
| expression of a bacillus thuringiensis cryia(c) gene in transgenic peanut plants and its efficacy against lesser cornstalk borer. | the invasion of peanut (arachis hypogaea l.) pods and seeds by aflatoxin-forming species of aspergillus is linked to injury by the lesser cornstalk borer and frequently causes a severe reduction in crop quality. the lesser cornstalk borer is susceptible to the lepidopteran-active bacillus thuringiensis insecticidal crystal protein. we have introduced a codon-modified bacillus thuringiensis cryia(c) gene into peanut using microprojectile bombardment. the toxin-coding region of a bt cryia(c) gene ... | 1997 | 9090064 |
| acrosome-intact boar spermatozoa initiate binding to the homologous zona pellucida in vitro. | the acrosome reaction is a prerequisite for zona pellucida penetration by mammalian spermatozoa. in some species, the sperm undergo the acrosome reaction before binding to the zona pellucida, and in other species only acrosome-intact sperm can initiate binding to the zona. in the present investigation, we addressed the question whether acrosome-intact or acrosome-reacted boar sperm initiate binding to the pig zona pellucida by studying the acrosomal status of sperm bound to zonae pellucidae. our ... | 1997 | 9116143 |
| lectin-binding glycoconjugates in the subfornical organ of the rat. | in the rat subfornical organ (sfo), lectin-binding glycoconjugates were histochemically examined by 9 biotinylated lectins using a streptavidin-biotin peroxidase system. the strong or moderate binding of canavalia ensiformis (con a), lens culinaris (lca) and phaseolus vulgaris erythroagglutinin (epha) indicated overall distributions of high mannose, intermediate, hybrid n-linked, and non-bisected, bi/triantennate n-linked complex oligosaccharides in the rat sfo. nonsialylated terminal n-acetylgl ... | 1997 | 9128064 |
| contribution of quantitative lectin histochemistry to characterizing well-differentiated, dedifferentiated and poorly differentiated liposarcomas. | to find new diagnostic markers in the group of lipomatous tumors. | 1997 | 9196804 |
| presence of vicia graminea lectin- and vicia unijuga lectin-binding (vgu) glycoprotein in human amniotic fluid and some of its chemical and serological properties. | vicia graminea- and vicia unijuga-binding glycoprotein (vgu glycoprotein) has been reported as a malignant tumor-associated antigen, which is found in various kinds of malignant tumor-tissues and ascitic and cyst fluids of malignant tumor patients, but not found in 20 kinds of normal human tissues. the glycoprotein reacts with anti-n lectins of vicia-graminea (vga) and vicia unijuga (vua), but does not react with anti-blood group m and n sera. the existence of vgu glycoprotein in human amniotic ... | 1997 | 9202424 |
| assessment of the acrosomal status of membrane-intact ram spermatozoa after freezing and thawing, by simultaneous lectin/hoechst 33258 staining. | we have evaluated the effect of freezing and thawing on the acrosomal status of ram spermatozoa, especially those that withstood cryopreservation as assessed by membrane integrity. to this end, we performed simultaneous lectin/hoechst 33258 staining, and compared the ability of three fluoresceinated lectins. ram spermatozoa were treated with fluorescein isothiocyanate-labelled pisum sativum lectin (psa), fluorescein isothiocyanate-labelled arachis hypogea lectin (pna) and fluorescein isothiocyan ... | 1997 | 9231240 |
| investigation of vicia graminea lectin- and vicia unijuga lectin-binding glycoproteins as novel oncofetal antigens in cyst and ascitic fluids of human ovarian tumors in benign, dysplastic, and malignant stages. | perchloric acid-soluble fractions (pasfs) were obtained from cyst fluids of human benign ovarian mucinous cystadenoma, benign dermoid cyst, "borderline" mucinous cystadenoma, and malignant ovarian clear cell carcinoma, and from fluids of malignant embryonal carcinoma and malignant serous cystadenocarcinoma. pasfs from benign lesions did not react with arachis hypogaea lectin (pna), vicia graminea lectin (vga), or vicia unijuga lectin (vua). pasfs from "borderline" lesions reacted with pna but di ... | 1997 | 9232320 |
| histochemical study of the epithelia of nasal polyps by biotinylated lectins and neoglycoprotein. a comparison with the normal human respiratory epithelium. | formalin-fixed, paraffin-embedded sections from human nasal polyps and the normal respiratory epithelium were glycohistochemically investigated. three biotinylated lectins were used: peanut (arachis hypogaea) agglutinin (pna) which, binds to terminal galactose (beta 1-3) n-acetylgalactosamine residues that can be unmasked by a neuraminidase digestion; wheat germ (triticum vulgare) agglutinin (wga), which binds to n-acetylglucosamine and n-acetylneuraminic acids; and gorse seed (ulex europaeus) a ... | 1997 | 9253585 |
| cbp70, a glycosylated nuclear lectin. | some years ago, a lectin designated cbp70 that recognized glucose (glc) but had a stronger affinity for n-acetylglucosamine (glcnac), was first isolated from hl60 cell nuclei. recently, a cytoplasmic form of this lectin was described, and one 82 kda nuclear ligand was characterized for the nuclear cbp70. in the present study, the use of pronase digestion and the trifluoromethanesulphonic acid (tfms) procedure strongly suggest that the nuclear and the cytoplasmic cbp70 have a same 23 kda polypept ... | 1997 | 9257193 |