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integrative "omic" analysis reveals distinctive cold responses in leaves and roots of strawberry, fragaria × ananassa 'korona'.to assess underlying metabolic processes and regulatory mechanisms during cold exposure of strawberry, integrative "omic" approaches were applied to fragaria × ananassa duch. 'korona.' both root and leaf tissues were examined for responses to the cold acclimation processes. levels of metabolites, proteins, and transcripts in tissues from plants grown at 18°c were compared to those following 1-10 days of cold (2°c) exposure. when leaves and roots were subjected to gc/tof-ms-based metabolite profi ...201526528299
mapping qtl associated with verticillium dahliae resistance in the cultivated strawberry (fragaria × ananassa).a biparental cross of octoploid strawberry segregating for resistance to verticillium dahliae, the causative agent of verticillium wilt, was screened under field conditions for three seasons. average wilt scores were significantly associated with multiple qtl, which were mostly significant across all years. markers significantly associated with the traits were used to screen material with known wilt resistance and susceptibility phenotypes. a clear and statistically significant relationship was ...201526504565
transcriptome profiling of postharvest strawberry fruit in response to exogenous auxin and abscisic acid.auxin and abscisic acid regulate strawberry fruit ripening and senescence through cross-talk of their signal transduction pathways that further modulate the structural genes related to physico-chemical properties of fruit. the physiological and transcriptomic changes in harvested strawberry fruits in responses to iaa, aba and their combination were analyzed. exogenous iaa delayed the ripening process of strawberries after harvest while aba promoted the postharvest ripening. however, treatment wi ...201626373937
temperature affects long-term productivity and quality attributes of day-neutral strawberry for a space life-support system.strawberry (fragaria x ananassa l.) is a promising candidate crop for space life-support systems with desirable sensory quality and health attributes. day-neutral cultivars such as 'seascape' are adaptable to a range of photoperiods, including short days that would save considerable energy for crop lighting without reductions in productivity or yield. since photoperiod and temperature interact to affect strawberry growth and development, several diurnal temperature regimes were tested under a sh ...201526177848
central role of fagamyb in the transition of the strawberry receptacle from development to ripening.the receptacle of the strawberry (fragaria × ananassa) fruit accounts for the main properties of the ripe fruit for human consumption. as it ripens, it undergoes changes similar to other fruits in sugar : acid ratio, volatile production and cell wall softening. however, the main regulators of this process have not yet been reported. the white stage marks the initiation of the ripening process, and we had previously reported a peak of expression for a fagamyb gene. transient silencing of fagamyb ...201526010039
identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (fragaria × ananassa) fruits.new modulators of the strawberry flavonoid pathway were identified through correlation network analysis. the transcriptomes of red, ripe fruit from two parental lines and 14 of their progeny were compared, and uncharacterized transcripts matching the expression patterns of known flavonoid-pathway genes were identified. fifteen transcripts corresponded with putative transcription factors, and several of these were examined experimentally using transient expression in developing fruits. the result ...201525979996
an r2r3-myb transcription factor regulates eugenol production in ripe strawberry fruit receptacles.eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. in ripe strawberry (fragaria × ananassa) fruit receptacles, eugenol is biosynthesized by eugenol synthase (faegs2). however, the transcriptional regulation of this process is still unknown. we have identified and functionally characterized an r2r3 myb transcription factor (emission of benzenoid ii [faeobii]) that seems to be the orthologous gene of pheobii from petunia hybrida, which contributes to the regulat ...201525931522
'niigata s3' is a new strawberry cultivar suitable for forcing culture under low temperature and insolation conditions.'niigata s3' is a new strawberry (fragaria × ananassa duch.) cultivar that is early flowering and possesses high soluble solid content and good coloration. it was selected from a cross between kei812 (seed parent) and 'asuka-ruby' (pollen parent). the first harvest date of 'niigata s3' was december 27, 34 days earlier than 'echigohime' and 9 days earlier than 'asuka-ruby' (means of 2007 and 2008). the marketable yield of 'niigata s3' was 85% of 'echigohime', 107% of 'asuka-ruby', while the early ...201425914600
recently fixed carbon allocation in strawberry plants and concurrent inorganic nitrogen uptake through arbuscular mycorrhizal fungi.most crop species form a symbiotic association with arbuscular mycorrhizal (am) fungi, receiving plant photosynthate and exchanging nutrients from the soil. the plant carbon (c) allocation to am fungi and the nitrogen feedback are rarely studied together. in this study, a dual (13)co2 and (15)nh4(15)no3 pulse labeling experiment was carried out to determine the allocation of recent photosynthates to mycorrhizal hyphae and the translocation of n absorbed by hyphae to strawberry plants. plants wer ...201525841208
strawberry as a health promoter: an evidence based review.since a high intake of fruits and vegetables is inversely related to the incidence of several degenerative diseases, the importance of a balanced diet in relation to human health has received increased consumer attention worldwide. strawberries (fragaria x ananassa, duch.) are a rich source of a wide variety of nutritive compounds such as sugars, vitamins, and minerals, as well as non-nutritive, bioactive compounds such as flavonoids, anthocyanins and phenolic acids. all of these compounds exert ...201525803191
developmental stages of cultivated strawberry flowers in relation to chilling sensitivity.environmental factors affecting flower development may limit the yields of fruiting crops worldwide. in temperate regions, chilling temperatures during flower development can compromise fruit production, but their negative effects vary depending on the differing susceptibilities of each developmental stage. the cultivated strawberry (fragaria× ananassa duch.) is widely grown worldwide but financial returns are influenced by sudden shifts to chilling temperatures occurring during the cropping cyc ...201525661200
effect of strawberry (fragaria × ananassa) leaf extract on diabetic nephropathy in rats.diabetic nephropathy is a clinical syndrome characterized by albuminuria, hypertension and progressive renal insufficiency. the aim of this study was to investigate the effect of strawberry (fragaria × ananassa) leaf extract on diabetic nephropathy in rats. streptozotocin (stz) diabetic rats were orally treated with three doses (50, 100 and 200 mg/kg) of strawberry leaf extract for 30 days. nephropathy biomarkers in plasma and kidney were examined at the end of the experiment. the three doses of ...201525645466
sucrose nonfermenting1-related protein kinase2.6, an ortholog of open stomata1, is a negative regulator of strawberry fruit development and ripening.whereas the regulatory mechanisms that direct fruit ripening have been studied extensively, little is known about the signaling mechanisms underlying this process, especially for nonclimacteric fruits. in this study, we demonstrated that a sucrose nonfermenting1-related protein kinase2, designated as fasnrk2.6, is a negative regulator of fruit development and ripening in the nonclimacteric fruit strawberry (fragaria × ananassa) and can also mediate temperature-modulated strawberry fruit ripening ...201525609556
variation in nutritional quality and chemical composition of fresh strawberry fruit: combined effect of cultivar and storage.bioclimatic air ionisation system (bi) works by neutralising air pollutants and microorganisms by means of oxidation with "activated oxygen". we investigated the effects of storage on changes in weight loss, chemical and sensory fruit properties in eight cultivars of strawberries (fragaria x ananassa duch.). all cultivars were evaluated for their standard parameters of quality (soluble solids content, total acidity, vitamin c content, total antioxidant activity - tac, total phenolic and anthocya ...201525575487
nontargeted metabolite profiles and sensory properties of strawberry cultivars grown both organically and conventionally.strawberry (fragaria × ananassa duch.) contains many secondary metabolites potentially beneficial for human health, and several of these compounds contribute to strawberry sensory properties, as well. in this study, three strawberry cultivars grown both conventionally and organically were subjected to nontargeted metabolite profiling analysis with lc-qtof-esi-ms and to descriptive sensory evaluation by a trained panel. combined metabolome and sensory data (pls model) revealed that 79% variation ...201525569122
effects of preharvest ultraviolet-c irradiation on fruit phytochemical profiles and antioxidant capacity in three strawberry (fragaria × ananassa duch.) cultivars.ultraviolet-c (uv-c) has proven effective in extending shelf-life, reducing disease incidence and increasing the levels of health-promoting compounds in several crops. while most studies were conducted at the postharvest stage, our study examined the effect of preharvest uv-c application in three strawberry cultivars (fragaria × ananassa duch. 'albion', 'charlotte' and 'seascape'). uv-c treatment was applied from the onset of flowering until the fruits reached commercial maturity on plants grown ...201525546470
light and abscisic acid independently regulated famyb10 in fragaria × ananassa fruit.light and aba independently regulated anthocyanin biosynthesis via activation of famyb10 expression. famyb10 accelerated anthocyanin synthesis of pelargonidin 3-glucoside and cyanidin 3-glucoside during strawberry fruit ripening. light is an integral factor in fruit ripening. ripening in non-climacteric fruit is also effected by the plant hormone abscisic acid (aba). however, how light and/or aba regulate fruit ripening processes, such as strawberry color development remains elusive. results of ...201525534946
effects of salinity and nitrogen supply on the quality and health-related compounds of strawberry fruits (fragaria × ananassa cv. primoris).different nitrogen inputs and/or development under adverse water conditions (water stress/low quality and/or high salinity/electrical conductivity), such as those prevailing in almeria (mediterranean coast, south-east spain), may affect overall fruit and vegetable quality. this study evaluated the influence of salinity and nitrogen reduction in hydroponic nutrient solution on strawberry fruit quality and nutritional compounds (fragaria × ananassa duch., cv. primoris).201525471904
strawberry (fragaria × ananassa).genetic transformation in strawberry (fragaria spp.) can be achieved by using the agrobacterium-mediated procedure on leaves from in vitro proliferated shoots. regardless of the sufficient regeneration levels achieved from leaf explants of some commercial strawberry genotypes, the regeneration of transformed strawberry plants remains difficult and seems to be strongly genotype dependent. in fact, the main factors that play an important role in the success of strawberry genetic transformation are ...201525416261
colored plastic mulch microclimates affect strawberry fruit yield and quality.significant reduction of strawberry (fragaria × ananassa, duch.) fruit yield and quality, as a consequence of conventional cultivation method, is common in the caspian sea region, iran. recently, growers started using plastic mulches to overcome these shortcomings. plastic mulches have different thermal and radiation properties and could affect strawberry fruit yield and quality. in the present study, the effect of different colored plastic mulches (black, red, and white) along with conventional ...201525348886
fapod27 functions in the metabolism of polyphenols in strawberry fruit (fragaria sp.).the strawberry (fragaria × ananassa) is one of the most preferred fresh fruit worldwide, accumulates numerous flavonoids but has limited shelf life due to excessive tissue softening caused by cell wall degradation. since lignin is one of the polymers that strengthen plant cell walls and might contribute to some extent to fruit firmness monolignol biosynthesis was studied in strawberry fruit. cinnamoyl-coa reductase (ccr), cinnamyl alcohol dehydrogenase (cad), and a peroxidase (pod27) gene were s ...201425346738
evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis.this study evaluated the effect of the solvent on the extraction of antioxidant compounds from black mulberry (morus nigra), blackberry (rubus ulmifolius) and strawberry (fragaria x ananassa). different extracts of each berry were evaluated from the determination of total phenolic content, anthocyanin content and antioxidant capacity, and data were applied to the principal component analysis (pca) to gain an overview of the effect of the solvent in extraction method.201425246942
inoculation of drought-stressed strawberry with a mixed inoculum of two arbuscular mycorrhizal fungi: effects on population dynamics of fungal species in roots and consequential plant tolerance to water deficiency.the effect of inoculation with two arbuscular mycorrhizal fungi (amf) on growth and drought tolerance of cultivated strawberry (fragaria × ananassa) was studied. three treatments (a single treatment either of funneliformis mosseae beg25, funneliformis geosporus beg11 or a 50:50 mixed inoculation treatment of both species) were compared to uninoculated plants. species-specific primers for qpcr quantification of f. geosporus and f. mosseae dna were developed to quantify the relative abundance of e ...201525186649
polyphenols in strawberry (fragaria × ananassa) leaves induced by plant activators.strawberry leaves contain high amounts of diverse phenolic compounds potentially possessing defensive activities against microbial pathogens and beneficial properties for human health. in this work, young strawberry plants were treated with two plant activators, s-methylbenzo-1,2,3-thaidiazole-7-carbothiate (bth) and birch wood distillate. phenolic compounds from activator-treated and control leaves were subjected to quantitative analyses by hplc-dad, hplc-esi-ms, and microqtof esi-ms. thirty-tw ...201424819677
identification of a strawberry flavor gene candidate using an integrated genetic-genomic-analytical chemistry approach.there is interest in improving the flavor of commercial strawberry (fragaria × ananassa) varieties. fruit flavor is shaped by combinations of sugars, acids and volatile compounds. many efforts seek to use genomics-based strategies to identify genes controlling flavor, and then designing durable molecular markers to follow these genes in breeding populations. in this report, fruit from two cultivars, varying for presence-absence of volatile compounds, along with segregating progeny, were analyzed ...201424742080
the effect of floral resources on parasitoid and host longevity: prospects for conservation biological control in strawberries.the strawberry tortricid, acleris comariana lienig and zeller (lepidoptera: tortricidae) is an important pest in danish strawberry production. its most common parasitoid is copidosoma aretas (walker) (hymenoptera: chalcidoidea: encyrtidae). to identify selective flowering plants that could be used to increase functional biodiversity, the longevity of c. aretas and its host a. comariana was assessed on 5 flowering species: buckwheat, fagopyrum esculentum moench (caryophyllales: polygonaceae); bor ...201324738921
biology of the two-spotted spider mite on strawberry plants.the two-spotted spider mite, tetranychus urticae koch, is commonly found on strawberry crops (fragaria x ananassa). strawberry plants have defensive mechanisms, which in turn influence the behavior of herbivores. the oviposition and development of the two-spotted spider mite were evaluated on the leaf disks of the cultivars 'aromas,' 'camarosa,' 'camino real,' 'diamante,' 'diamante 10,' 'diamante 50,' 'festival,' and 'seascape.' it was observed that on cultivars such as 'aromas,' 'camarosa,' and ...201427193526
potential role of pectate lyase and ca(2+) in the increase in strawberry fruit firmness induced by short-term treatment with high-pressure co2.postharvest treatment with high-pressure co2 helps to control decay and increase firmness in strawberries. increases in firmness occurred through modification of calcium binding to cell wall. however, the mechanism(s) involved in ca(2+) migration to pectic polymers and other physiological events associated with the maintenance of increased firmness are not clearly understood. the focus of this study was to find potential mechanism(s) that are associated with calcium movement, increases in firmne ...201424592970
doxorubicin-induced oxidative stress in rats is efficiently counteracted by dietary anthocyanin differently enriched strawberry (fragaria × ananassa duch.).this study investigated the effects of two different strawberry cultivars, adria and sveva, against doxorubicin (dox)-induced toxicity in rats. a controlled dietary intervention was conducted over 16 weeks with four groups: (i) normal diet; (ii) normal diet + dox injection; (iii) adria supplementation + dox injection; and (iv) sveva supplementation + dox injection. sveva presented higher total antioxidant capacity value and phenol and and vitamin c levels than adria, which in turn presented high ...201424580025
strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.fresh strawberries (fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor. in this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating. analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach. sweetness intensity, flavor intensity, and texture liki ...201424523895
sodium hydrosulfide induces systemic thermotolerance to strawberry plants through transcriptional regulation of heat shock proteins and aquaporin.temperature extremes represent an important limiting factor to plant growth and productivity. the present study evaluated the effect of hydroponic pretreatment of strawberry (fragaria x ananassa cv. 'camarosa') roots with an h2s donor, sodium hydrosulfide (nahs; 100 μm for 48 h), on the response of plants to acute heat shock treatment (42°c, 8 h).201424499299
strawberry and human health: effects beyond antioxidant activity.the usefulness of a diet rich in vegetables and fruits on human health has been widely recognized: a high intake of antioxidant and bioactive compounds may in fact play a crucial role in the prevention of several diseases, such as cancer, cardiovascular, neurodegenerative, and other chronic pathologies. the strawberry (fragaria × ananassa duch.) possesses a remarkable nutritional composition in terms of micronutrients, such as minerals, vitamin c, and folates, and non-nutrient elements, such as ...201424450925
somatic embryo-like structures of strawberry regenerated in vitro on media supplemented with 2,4-d and bap.somatic embryo-like structures (sels) were produced in vitro from leaf disk and petiole explants of two cultivars of strawberry (fragaria x ananassa duch) on murashige and skoog medium with different concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-d), 6-benzylaminopurine (bap) and sucrose to check the embryonic nature of these structures histologically. a large number of sels could be regenerated in both cultivars on media with 2-4 mg l(-1) 2,4-d in combination with 0.5 -1 ...201324377134
heteromerization of pip aquaporins affects their intrinsic permeability.the plant aquaporin plasma membrane intrinsic proteins (pip) subfamily represents one of the main gateways for water exchange at the plasma membrane (pm). a fraction of this subfamily, known as pip1, does not reach the pm unless they are coexpressed with a pip2 aquaporin. although ubiquitous and abundantly expressed, the role and properties of pip1 aquaporins have therefore remained masked. here, we unravel how fapip1;1, a fruit-specific pip1 aquaporin from fragaria x ananassa, contributes to th ...201424367080
improved regeneration and transformation protocols for three strawberry cultivars.strawberry (fragaria × ananassa) is an economically important soft fruit crop with polyploid genome which makes the breeding of new cultivars difficult. simple and efficient method for transformation and regeneration is required for cultivars improvement in strawberry. in the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry plants, i.e., festival, sweet charly and florida via direct organogenesis using the in vitro juvenile leaves as explants. e ...201724322545
can mass trapping reduce thrips damage and is it economically viable? management of the western flower thrips in strawberry.the western flower thrips frankliniella occidentalis (pergande) (thysanoptera: thripidae) is a cosmopolitan, polyphagous insect pest that causes bronzing to fruit of strawberry (fragaria x ananassa). the main aim of this study was to test whether mass trapping could reduce damage and to predict whether this approach would be economically viable. in semi-protected strawberry crops, mass trapping of f. occidentalis using blue sticky roller traps reduced adult thrips numbers per flower by 61% and f ...201324282554
dissection of the octoploid strawberry genome by deep sequencing of the genomes of fragaria species.cultivated strawberry (fragaria x ananassa) is octoploid and shows allogamous behaviour. the present study aims at dissecting this octoploid genome through comparison with its wild relatives, f. iinumae, f. nipponica, f. nubicola, and f. orientalis by de novo whole-genome sequencing on an illumina and roche 454 platforms. the total length of the assembled illumina genome sequences obtained was 698 mb for f. x ananassa, and ∼200 mb each for the four wild species. subsequently, a virtual reference ...201424282021
myb10 plays a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening of fragaria x ananassa fruits.this work characterized the role of the r2r3-myb10 transcription factor (tf) in strawberry fruit ripening. the expression of this tf takes place mainly in the fruit receptacle and is repressed by auxins and activated by abscisic acid (aba), in parallel to the ripening process. anthocyanin was not produced when famyb10 expression was transiently silenced in fruit receptacles. an increase in famyb10 expression was observed in water-stressed fruits, which was accompanied by an increase in both aba ...201424277278
conserved changes in the dynamics of metabolic processes during fruit development and ripening across species.computational analyses of molecular phenotypes traditionally aim at identifying biochemical components that exhibit differential expression under various scenarios (e.g. environmental and internal perturbations) in a single species. high-throughput metabolomics technologies allow the quantification of (relative) metabolite levels across developmental stages in different tissues, organs, and species. novel methods for analyzing the resulting multiple data tables could reveal preserved dynamics of ...201424243932
genome-wide identification and analysis of fk506-binding protein family gene family in strawberry (fragaria × ananassa).the fk506 binding proteins (fkbps) are abundant and ubiquitous proteins belonging to the large peptidyl-prolylcis-trans isomerase superfamily. fkbps are known to be involved in many biological processes including hormone signaling, plant growth, and stress responses through a chaperone or an isomerization of proline residues during protein folding. the availability of complete strawberry genome sequences allowed the identification of 23 fkbp genes by hmmer and blast analysis. chromosome scaffold ...201424230972
shallot aphids, myzus ascalonicus, in strawberry: biocontrol potential of three predators and three parasitoids.the parasitization capacity of 3 parasitoids and the predation capacity of 3 predators towards the shallot aphid, myzus ascalonicus doncaster (homoptera: aphididae), on strawberry, fragaria x ananassa duchesne (rosales: rosaceae) cv. honeoye, were examined in laboratory experiments. in petri dish assays, both aphidius colemani viereck (hymenoptera: aphidiidae) and a. ervi haliday readily stung shallot aphids, with no significant difference in stinging frequency between the two species. a. ervi i ...201324224712
determination of free amino acids and 18 elements in freeze-dried strawberry and blueberry fruit using an amino acid analyzer and icp-ms with micro-wave digestion.the objective of this study was to investigate the level of 18 trace elements of two freeze-dried samples from the blueberry (vaccinium corymbosum) and the strawberry (fragaria × ananassa). the total free amino acid composition in the blueberry and strawberry was determined by an amino acid analyzer. eleven free amino acids were found in both berries. the trace elements in each dried fruit sample were determined by icp-ms with microwave digestion. the linearity range of the standard curves was 0 ...201424206704
premature and ectopic anthocyanin formation by silencing of anthocyanidin reductase in strawberry (fragaria × ananassa).strawberry (fragaria × ananassa) is a fruit crop with a distinct biphasic flavonoid biosynthesis. whereas, in the immature receptacle, high levels of proanthocyanidins accumulate, which are associated with herbivore deterrence and pathogen defense, the prominent color-giving anthocyanins are primarily produced in ripe 'fruits' helping to attract herbivores for seed dispersal. here, constitutive experimental down-regulation of one branch of proanthocyanidin biosynthesis was performed. as a result ...201424117941
phototropin 2 is involved in blue light-induced anthocyanin accumulation in fragaria x ananassa fruits.anthocyanins are widespread, essential secondary metabolites in higher plants during color development in certain flowers and fruits. in strawberries, anthocyanins are also key contributors to fruit antioxidant capacity and nutritional value. however, the effects of different light qualities on anthocyanin accumulation in strawberry (fragaria x ananassa, cv. sachinoka) fruits remain elusive. in the present study, we showed the most efficient increase in anthocyanin content occurred by blue light ...201323982948
identification and validation of reference genes for transcript normalization in strawberry (fragaria × ananassa) defense responses.strawberry (fragaria spp) is an emerging model for the development of basic genomics and recombinant dna studies among rosaceous crops. functional genomic and molecular studies involve relative quantification of gene expression under experimental conditions of interest. accuracy and reliability are dependent upon the choice of an optimal reference control transcript. there is no information available on validated endogenous reference genes for use in studies testing strawberry-pathogen interacti ...201323940602
protein synthesis inhibition activity by strawberry tissue protein extracts during plant life cycle and under biotic and abiotic stresses.ribosome-inactivating proteins (rips), enzymes that are widely distributed in the plant kingdom, inhibit protein synthesis by depurinating rrna and many other polynucleotidic substrates. although rips show antiviral, antifungal, and insecticidal activities, their biological and physiological roles are not completely understood. additionally, it has been described that rip expression is augmented under stressful conditions. in this study, we evaluated protein synthesis inhibition activity in part ...201323892598
evaluation of the sensory characteristics of strawberry cultivars throughout the harvest season using projective mapping.strawberry breeding programs should be able to rely on valid and simple methodologies for evaluating sensory quality of new cultivars. in this context, the aim of the present work was to evaluate the applicability of a simple sensory methodology, named projective mapping, to evaluate the sensory characteristics of strawberry cultivars and advanced selections throughout the harvest season. three strawberry cultivars and two advanced selections were evaluated by an untrained assessor panel using p ...201423868389
metabolic interaction between anthocyanin and lignin biosynthesis is associated with peroxidase faprx27 in strawberry fruit.plant phenolics have drawn increasing attention due to their potential nutritional benefits. although the basic reactions of the phenolics biosynthetic pathways in plants have been intensively analyzed, the regulation of their accumulation and flux through the pathway is not that well established. the aim of this study was to use a strawberry (fragaria × ananassa) microarray to investigate gene expression patterns associated with the accumulation of phenylpropanoids, flavonoids, and anthocyanins ...201323835409
[luminescence characteristics of strawberry leaves in early stages of their injury by arachnoidal mite].in this work, we have investigated how the luminescence characteristics and a total content of antioxidants in leaves of strawberry (fragaria x ananassa duch.) change after infestation of plants by spider mite (tetranychus atlanticus mcgregor). at the earlier stage of plant injury, the chlorophyll concentration remained unchanged. however, the total amount of antioxidants decreased and the intensity of high-temperature band (60 divided by 100 degrees c) of thermoluminescence increased, indicatin ...201323755560
antisense expression of peach mildew resistance locus o (ppmlo1) gene confers cross-species resistance to powdery mildew in fragaria x ananassa.powdery mildew (pm) is one of the major plant pathogens. the conventional method of pm control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. pm remains a major scourge for rosaceae crops where breeding approaches mainly resort to gene-for-gene resistance. we have tested an alternate source of pm resistance in rosaceae. mildew resistance locus o (mlo) has been well studied in barley due to its role in imparting broad spectrum re ...201323728780
induced leaf intercellular co₂, photosynthesis, potassium and nitrate retention and strawberry early fruit formation under macronutrient limitation.relationships between induced high leaf intercellular co₂ concentrations, leaf k⁺ and no₃⁻ ion movement and early fruit formation under macronutrient limitation are not well understood. we examined the effects and interactions of reduced k/n input treatments on leaf intercellular co₂, photosynthesis rate, carboxylation and water use efficiency, berry formation as well as leaf/fruit k⁺, no₃⁻ and photosynthate retention of strawberry (fragaria × ananassa duch.) to enhance low-input agriculture. th ...201323686470
blotting assisted by heating and solvent extraction for desi-ms imaging.imprints of potato sprout (solanum tuberosum l.), gingko leaves (gingko biloba l.) and strawberries (fragaria x ananassa duch.) were successfully imaged by desorption electrospray ionization mass spectrometry (desi-ms) on tlc plates through blotting assisted by heating and/or solvent extraction. ion images showing the distribution of significant compounds such as glycoalkaloid toxins in potato sprout, ginkgolic acids and flavonoids in ginkgo leaves, and sugars and anthocyanidin in strawberry wer ...201323605686
hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways.hydrogen sulfide (h2s) has been recently found to act as a potent priming agent. this study explored the hypothesis that hydroponic pretreatment of strawberry (fragaria × ananassa cv. camarosa) roots with a h2s donor, sodium hydrosulfide (nahs; 100 μm for 48 h), could induce long-lasting priming effects and tolerance to subsequent exposure to 100mm naci or 10% (w/v) peg-6000 for 7 d. hydrogen sulfide pretreatment of roots resulted in increased leaf chlorophyll fluorescence, stomatal conductance ...201323567865
bioactive compounds and antioxidant capacity of camarosa and selva strawberries (fragaria x ananassa duch.).strawberries represent an important source of bioactive compounds due to their vitamin c and phenolic compound levels, which present high antioxidant effects, beneficial for the maintenance of consumer's health. argentina is the second largest strawberry producer in the common market of the southern cone (mercosur), covering the main export destinations of argentinian strawberries, i.e., canada, united states, and european union. information about the bioactive compound occurrence and antioxidan ...201328239102
sucrose functions as a signal involved in the regulation of strawberry fruit development and ripening.fleshy fruits are classically divided into climacteric and nonclimacteric types. it has long been thought that the ripening of climacteric and nonclimacteric fruits is regulated by ethylene and abscisic acid (aba), respectively. here, we report that sucrose functions as a signal in the ripening of strawberry (fragaria × ananassa), a nonclimacteric fruit. pharmacological experiments, as well as gain- and loss-of-function studies, were performed to demonstrate the critical role of sucrose in the r ...201323425297
genetic and environmental effects on tannin composition in strawberry (fragaria × ananassa) cultivars grown in different european locations.strawberry cultivars grown at different locations in europe showed genotype- and environment-dependent variation in total phenol and anthocyanin contents. this study focused on the compositional diversity of tannins from these cultivars using a high-throughput liquid chromatography-mass spectrometric (lc-ms) technique. cultivars grown in germany showed differences in the relative amounts of proanthocyanidins (pacs) and ellagitannins (ets). cultivars grown at three different european locations sh ...201323301905
physical attributes and chemical composition of organic strawberry fruit (fragaria x ananassa duch, cv. albion) at six stages of ripening.organic strawberry fruits (cv. 'albion') were harvested at six different ripening stages and evaluated for physical and chemical parameters. biometrical characteristics and moisture content did not change significantly during ripening. total soluble solids, ph and colour development increased while titratable acidity and fruit firmness decreased 14.7% and 91%, respectively. fructose, glucose, and sucrose followed similar tendencies. final contents of these sugars were 2323.4, 1988.5, and 1578.4 ...201323265501
biology and fertility life table of the green aphid chaetosiphon fragaefolli on strawberry cultivars.our objective was to study the biology and develop a fertility life table for the aphid chaetosiphon fragaefolli (cockerell) (hemiptera: aphididae) on leaves of strawberry, fragaria × ananassa, duchesne ex rozier (rosales: rosaceae), of the cultivars albion, aromas, camarosa, camino real, diamante, earlibrite, and saborosa. this study was conducted under controlled conditions: 25 ± 1 (°)c, 70 ± 10% rh, and 12:12 l:d . arenas were set up consisting of leaves inside petri dishes containing 3% agar ...201222958325
solution structure of the strawberry allergen fra a 1.the pr10 family protein fra a 1e from strawberry (fragaria x ananassa) is down-regulated in white strawberry mutants, and transient rnai (rna interference)-mediated silencing experiments confirmed that fra a 1 is involved in fruit pigment synthesis. in the present study, we determined the solution structure of fra a 1e. the protein fold is identical with that of other members of the pr10 protein family and consists of a seven-stranded antiparallel β-sheet, two short v-shaped α-helices and a long ...201222913709
effects of elevated co2 and temperature on yield and fruit quality of strawberry (fragaria × ananassa duch.) at two levels of nitrogen application.we investigated if elevated co(2) could alleviate the negative effect of high temperature on fruit yield of strawberry (fragaria × ananassa duch. cv. toyonoka) at different levels of nitrogen and also tested the combined effects of co(2), temperature and nitrogen on fruit quality of plants cultivated in controlled growth chambers. results show that elevated co(2) and high temperature caused a further 12% and 35% decrease in fruit yield at low and high nitrogen, respectively. the fewer infloresce ...201222911728
the potential impact of strawberry on human health.the strawberry (fragaria x ananassa, duch.) represents a relevant source of micronutrients, such as minerals, vitamin c, folate and phenolic substances, most of which are natural antioxidants and contribute to the high nutritional quality of the fruit. all these compounds are essential for health and, in particular, strawberry phenolics are best known for their antioxidant and anti-inflammatory action, and possess directly and indirectly antimicrobial, anti-allergy and anti-hypertensive properti ...201322788743
proteomic study of low-temperature responses in strawberry cultivars (fragaria x ananassa) that differ in cold tolerance.to gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of octoploid strawberry (fragaria × ananassa) cultivars that differ in freezing tolerance was conducted. four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar ('jonsok') and least-tolerant cultivar ('frida') were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to iden ...201222689892
phenolic compounds in strawberry (fragaria x ananassa duch.) fruits: composition in 27 cultivars and changes during ripening.phenolic compounds in fruits of 27 cultivars of strawberry (fragaria x ananassa duch.) grown in norway were characterised and quantified by hplc-dad-ms(n). total phenolic content, calculated as the sum of the individual compounds, varied 2.3-fold among cultivars, i.e., from 57 to 133mg/100g of fw. there were significant differences among cultivars in concentration of all phenolic compounds. the highest variation between cultivars was found for cinnamoyl glucose (0.6-24.9mg/100g of fw). concentra ...201226434267
development of colour and firmness in strawberry crops is uv light sensitive, but colour is not a good predictor of several quality parameters.strawberry (fragaria × ananassa duchesne var. elsanta) plants were grown in polytunnels covered with three polythene films that transmitted varying levels of ultraviolet (uv) light. fruit were harvested under near-commercial conditions and quality and yield were measured. during ripening, changes in the colour parameters of individual fruit were monitored, and the accuracy of using surface colour to predict other quality parameters was determined by analysing the correlation between colour and q ...201222517238
isolation and characterization of two yucca flavin monooxygenase genes from cultivated strawberry (fragaria × ananassa duch.).in strawberry (fragaria × ananassa duch.), auxin has been recognized as the main signal molecule coordinating the growth and initiation of ripening of fruits. the molecular mechanism regulating auxin biosynthesis in strawberry remains unknown. this project reports two yucca flavin monooxygenase genes fayuc1-2 isolated from cultivated strawberry. fayuc1 and fayuc2 are most homologous to atyuc6 and atyuc4, respectively. significant expression of fayuc1-2 is found in vegetative meristems and reprod ...201222491872
genetic analysis of strawberry fruit aroma and identification of o-methyltransferase faomt as the locus controlling natural variation in mesifurane content.improvement of strawberry (fragaria × ananassa) fruit flavor is an important goal in breeding programs. to investigate genetic factors controlling this complex trait, a strawberry mapping population derived from genotype '1392', selected for its superior flavor, and '232' was profiled for volatile compounds over 4 years by headspace solid phase microextraction coupled to gas chromatography and mass spectrometry. more than 300 volatile compounds were detected, of which 87 were identified by compa ...201222474217
nitrogen availability drives the effect of glomus intraradices on the growth of strawberry (fragaria x ananassa duch.) plants.on the one hand, the critical nitrogen (n) content curve allows the minimal n content necessary for maximum growth rate at any stage of crop development to be predicted. on the other hand, arbuscular mycorrhizal fungi (amf) transfer n from the soil to the plants and its growth and activity depends on the availability of soil n. our objective was to investigate how the availability of n in the soil affects growth and the accumulation of n in inoculated strawberry plants. root colonisation, dry ma ...201222331548
purification and identification of an ige suppressor from strawberry in an in vitro immunization system.we purified and identified an ige suppressor from the strawberry 'toyonoka', based on the decrease of ige production in in vitro immunization (ivi). gel filtration experiment indicated that fractions in a 15-48 kda range and <10 kda have an ige suppressive activity. furthermore, the fraction in 15-48 kda was subjected to chromatofocusing and found to have activities at isoelectric points, pi 6.0, 7.0, and 8.0-9.2. we focused on the active fractions of pi 8.0-9.2 and the purified a large amount o ...201222328134
photoprotective potential of strawberry (fragaria × ananassa) extract against uv-a irradiation damage on human fibroblasts.exposure to uv-a radiation is known to induce discrete lesions in dna and the generation of free radicals that lead to a wide array of skin diseases. strawberry (fragaria × ananassa) contains several polyphenols with strong antioxidant and anti-inflammatory activities. because the major representative components of strawberry are anthocyanins, these may significantly contribute to its properties. to test this hypothesis, methanolic extracts from the sveva cultivar were analyzed for anthocyanin c ...201222304566
a microsatellite linkage map for the cultivated strawberry (fragaria × ananassa) suggests extensive regions of homozygosity in the genome that may have resulted from breeding and selection.the linkage maps of the cultivated strawberry, fragaria × ananassa (2n = 8x = 56) that have been reported to date have been developed predominantly from aflps, along with supplementation with transferrable microsatellite (ssr) markers. for the investigation of the inheritance of morphological characters in the cultivated strawberry and for the development of tools for marker-assisted breeding and selection, it is desirable to populate maps of the genome with an abundance of transferrable molecul ...201222218676
dynamics of phytohormone and dna methylation patterns changes during dormancy induction in strawberry (fragaria × ananassa duch.).changes in endogenous phytohormone levels, dna methylation patterns, and expression levels of related genes during induction of dormancy in two strawberry cultivars, darselect and all star, were studied under controlled environmental conditions. at 12°c, regardless of day length, potted, runner-derived plants of both cultivars gradually exhibited morphological traits typical of dormancy after treatment for 8 weeks. these morphological changes were accompanied by a synchronous significant decline ...201221935696
first record of spintherophyta semiaurata (klug) (coleoptera: chrysomelidae) damaging strawberry flowers.spintherophyta semiaurata (klug) is reported for the first time damaging flowers of strawberry (fragaria x ananassa) in the municipality of são josé dos pinhais, state of parana, brazil.201121710039
quantitative trait loci and underlying candidate genes controlling agronomical and fruit quality traits in octoploid strawberry (fragaria × ananassa).breeding for fruit quality traits in strawberry (fragaria × ananassa, 2n = 8x = 56) is complex due to the polygenic nature of these traits and the octoploid constitution of this species. in order to improve the efficiency of genotype selection, the identification of quantitative trait loci (qtl) and associated molecular markers will constitute a valuable tool for breeding programs. however, the implementation of these markers in breeding programs depends upon the complexity and stability of qtls ...201121667037
variation in the amounts of selected volatiles in a model population of fragaria × ananassa duch. as influenced by harvest year.volatile metabolites are a basis for sensory and resistance traits of fragaria × ananassa . stability of expression is important for the selection of cultivars. for the first time, the stability of volatiles in a strawberry population after cross-combination of two distinct cultivars ('mieze schindler' × 'elsanta') has been investigated. in this work, environmentally caused variations in the synthesis of 18 volatiles were studied over two years using a model population of 158 clones. the stabili ...201121218780
a sepallata gene is involved in the development and ripening of strawberry (fragaria x ananassa duch.) fruit, a non-climacteric tissue.climacteric and non-climacteric fruits have traditionally been viewed as representing two distinct programmes of ripening associated with differential respiration and ethylene hormone effects. in climacteric fruits, such as tomato and banana, the ripening process is marked by increased respiration and is induced and co-ordinated by ethylene, while in non-climacteric fruits, such as strawberry and grape, it is controlled by an ethylene-independent process with little change in respiration rate. t ...201121115665
solar uvb response of bioactives in strawberry (fragaria × ananassa duch. l.): a comparison of protected and open-field cultivation.strawberries (fragaria × ananassa duch. cvs. everest, elsanta) were grown in a tunnel covered with two films, which were distinguished in their ultraviolet transparency, as well as under open-field conditions. one applied film was not transparent for uvb radiation, and the second film transmitted 70% of uvb radiation. during the present study, the nutritional value and quality parameters of the fruits were evaluated. strawberries were uv-unresponsive in view of the content of ascorbic acid and s ...201021086998
variation in preference and performance of frankliniella occidentalis (thysanoptera: thripidae) on three strawberry cultivars.western flower thrips, frankliniella occidentalis (pergande) (thysanoptera: thripidae), is a major pest of strawberry, causing substantial yield loss through direct feeding on the flowers and fruit. insecticides are the main method used for its control; however, f. occidentalis has developed resistance to insecticides from all major chemical classes. chemical control is not a long-term strategy and integrated pest management is required. this study determined whether f. occidentalis damage could ...201021061975
metabolic profiling of strawberry (fragaria x ananassa duch.) during fruit development and maturation.strawberry (fragaria × ananassa duch), a fruit of economic and nutritional importance, is also a model species for fleshy fruits and genomics in rosaceae. strawberry fruit quality at different harvest stages is a function of the fruit's metabolite content, which results from physiological changes during fruit growth and ripening. in order to investigate strawberry fruit development, untargeted (gc-ms) and targeted (hplc) metabolic profiling analyses were conducted. principal component analysis ( ...201121041374
incorporation of intraguild predation into a pest management decision-making tool: the case of thrips and two pollen-feeding predators in strawberry.action thresholds are traditionally based on the density of pests and the economic damage they cause to crops. pest damage assessments are usually made in a "sterile" environment, devoid of extenuating factors such as predators, parasitoids, and alternative food sources. recently, the effects of a predator or parasitoid species have been considered. however, interactions between natural enemy species (intraguild predation and interference), which are common in agricultural fields, have not been ...201020857715
characterization of faspt, a spatula gene encoding a bhlh transcriptional factor from the non-climacteric strawberry fruit.the involvement of basic-helix-loop-helix (bhlh) transcription factors in essential physiological and developmental processes is well established. although a lot of animal bhlh proteins were characterized functionally, much less bhlhs of plant origin have been studied so far. using a cdna-aflp approach, a ripening-related spatula gene was identified from strawberry fruit (fragaria × ananassa duch.), which encodes a bhlh protein. it is an orthologue of an arabidopsis spatula protein, which has an ...201620822914
assessment of the influence of different sample processing and cold storage duration on plant free proline content analyses.a method which is widely accepted for the analysis of free proline content in plant tissues is based on the use of 3% sulfosalicylic acid as an extractant, followed by spectrophotometric quantification of a proline-ninhydrin complex in toluene. however, sample preparation and storage may influence the proline actually measured. this may give misleading or difficult to compare data.201320603866
root colonisation by the arbuscular mycorrhizal fungus glomus intraradices alters the quality of strawberry fruits (fragaria x ananassa duch.) at different nitrogen levels.arbuscular mycorrhizal fungi (amf) increase the uptake of minerals from the soil, thus improving the growth of the host plant. nitrogen (n) is a main mineral element for plant growth, as it is an essential component of numerous plant compounds affecting fruit quality. the availability of n to plants also affects the amf-plant interaction, which suggests that the quality of fruits could be affected by both factors. the objective of this study was to evaluate the influence of three n treatments (3 ...201020572056
effect of different cultural systems on antioxidant capacity, phenolic content, and fruit quality of strawberries (fragaria x aranassa duch.).the effect of cultivation practices for controlling strawberry black root rot (brr) on fruit quality, antioxidant capacity, and flavonoid content in two strawberry cultivars allstar and chandler (fragaria x ananassa duch.) was evaluated. strawberry fruits used in this study were from plants grown in soils which had a prior history of brr and red stele, and had not been fumigated during the seven years prior to the study. results from this study showed that fruit from plants grown in compost sock ...200920560628
effect of bth on anthocyanin content and activities of related enzymes in strawberry after harvest.the effect of benzo-thiadiazole-7-carbothioic acid s-methyl ester (bth) at 0.2 g l(-1) on anthocyanin content and the enzymes involved in its metabolism such as glucose-6-phosphate dehydrogenase (g6pdh), shikimate dehydrogenase (skdh), tyrosine ammonia lyase (tal), phenylalanine ammonia lyase (pal), cinnamate-4-hydroxylase (c4h), 4-coumarate/coenzyme a ligase (4-cl), and dihydroflavonol 4-reductase (dfr) in strawberry (fragaria x ananassa duch.) fruit was investigated in this study. the result s ...201020377227
fare1: a transcriptionally active ty1-copia retrotransposon in strawberry.retrotransposons are ubiquitous in the plant kingdom and constitute a large fraction of many plant genomes. although most retrotransposons from plants were thought to be transcriptionally silent in somatic tissues, evidence of activity under certain conditions is available in some cases. in this study, a complete ltr retrotransposon was isolated from the cultivated strawberry (fragaria x ananassa) genome using genome walking. the element, named fare1, has all the features of a typical ty1-copia ...201020020171
the polygalacturonase fapg1 gene plays a key role in strawberry fruit softening.the loss of firm texture is one of the most characteristic physiological processes that occur during the ripening of fleshy fruits. it is generally accepted that the disassembly of primary cell wall and middle lamella is the main factor involved in fruit softening. in this process, polygalacturonase (pg) has been implicated in the degradation of the polyuronide network in several fruits. however, the minor effect of pg downregulation on tomato softening, reported during the nineties, minimized t ...200919820312
effects of sucrose preculture on cryopreservation by droplet-vitrification of strawberry cultivars and morphological stability of cryopreserved plants.the droplet-vitrification method was applied to shoot tips of micropropagated strawberry plants (fragaria x ananassa duch. cvs. 'senga sengana', 'korona' and 'aroma'). shoot tips of 2 - 3 mm in length were precultured in sucrose (0.1, 0.25, 0.5, 0.75, 1.0 m) enriched media for 24 and 48 h. subsequently, they were transferred into 6 microl droplets of pvs2 vitrification solution for 20 min and plunged into liquid nitrogen. rapid rewarming was done in liquid medium at room temperature. the highest ...200919750244
genotyping of strawberry (fragaria x ananassa duch.) cultivars by dna markers: interlaboratory study.fourteen japanese laboratories validated the reproducibility of genotyping by 25 cleavage amplified polymorphic sequence (caps) markers for discrimination of strawberry (fragaria x ananassa duch.) cultivars. both the sensitivity and specificity rate of 12 markers were 100%, those of another 12 were >95%, and those of 1 were >90%. these results indicate that the method of genotyping by the caps markers was highly reproducible and could provide a useful basis for practical identification of strawb ...200919610383
isolation of dna-methyltransferase genes from strawberry (fragaria x ananassa duch.) and their expression in relation to micropropagation.dna methylation can control gene expression and may also play a role in plant development. methylation of cytosine residues in dna is enzymatically catalyzed by dna methyltransferases. in this study, full-length genomic genes and cdnas of methyltransferase (met1) and domain-rearranged methyltransferase (drm) were isolated from strawberry (fragaria x ananassa duch.). two genomic clones (famet1a and famet1b) encoding met1 had open-reading frame of 4,695 and 4,671 nucleotides with two introns, resp ...200919565244
strawberry (fragaria x ananassa duch.) yield as affected by the soil ph.two-year trials (20062007) suggested that the use of calcium oxide (cao) on acid soils increased soil ph and yields in strawberry cultivars marmolada, selena and senga sengana, under the environmental conditions of cacak (western serbia). the highest yield was obtained when cao was applied at 750 kg ha-1 rate. further increase in rate up to 1,500 kg ha-1 did not show corresponding increase in yield; the result was a slight yield drop compared to the peak yield shown at 750 kg ha(1) rate. overall ...200919488630
nmr and uplc-qtof-ms/ms characterisation of novel phenylethanol derivatives of phenylpropanoid glucosides from the leaves of strawberry (fragaria x ananassa cv. jonsok).strawberry (fragaria x ananassa) is rich in polyphenols, particularly anthocyanins, flavonols, condensed tannins and ellagic tannins. in addition to the fruits, the leaves of strawberry also contain a wide range of phenolic compound classes, but have not been investigated to the same extent as the fruit.201319425115
developmental, genetic and environmental factors affect the expression of flavonoid genes, enzymes and metabolites in strawberry fruits.the influence of internal (genetic and developmental) and external (environmental) factors on levels of flavonoid gene transcripts, enzyme activity and metabolites was studied in fruit of six cultivated strawberry (fragaria x ananassa duch.) genotypes grown at two italian locations. gene expression and enzyme activity showed development- and genotype-associated patterns, revealing gene coordination. analysis clarified the regulation mechanism of the hydroxylation status of the b-ring of the majo ...200919422609
microrna expression profiles in conventional and micropropagated strawberry (fragaria x ananassa duch.) plants.micrornas (mirnas) are a class of small non-coding rnas which play a critical role in plant growth and development. to detect strawberry mirnas and discover the expression difference between conventional and micropropagated strawberry plants, we carried out the detection and quantification of strawberry mirnas by microarray. the main findings were that 74 mirnas were checked in strawberry plants and four mirna genes displayed clear expression difference between conventional and micropropagated s ...200919277667
recovery of plants from pea and strawberry meristems cryopreserved for 28 years.meristems isolated from in vitro strawberry shoot cultures (fragaria x ananassa duch. cv. redcoat) and in vitro germinated pea seedlings (pisum sativum l. cv. century) were treated with cryoprotectants, slowly frozen to -40 degree c (0.6 degree c per min for pea and 0.84 degree c per min for strawberry), and stored in liquid nitrogen in 1979. in 2007, several ampoules containing cryopreserved meristems of each species were thawed, cultured in vitro, and scored for viability. meristems which diff ...201319274310
gibberellin mediates daylength-controlled differentiation of vegetative meristems in strawberry (fragaria x ananassa duch).differentiation of long and short shoots is an important developmental trait in several species of the rosaceae family. however, the physiological mechanisms controlling this differentiation are largely unknown. we have studied the role of gibberellin (ga) in regulation of shoot differentiation in strawberry (fragaria x ananassa duch.) cv. korona. in strawberry, differentiation of axillary buds to runners (long shoot) or to crown branches (short shoot) is promoted by long-day and short-day condi ...200919210764
evolution of selected volatiles in chitosan-coated strawberries ( fragaria x ananassa ) during refrigerated storage.the effect of chitosan coating on the evolution of several volatile compounds relevant to the strawberry ( fragaria x ananassa cv. camarosa) aroma profile has been investigated. strawberries dipped in chitosan acetate solution at 1 or 1.5% (w/w) and uncoated controls were stored at 10 degrees c for 1 week. significant differences in aroma profile between coated and uncoated samples were observed. most importantly, the buildup of the off-flavors acetaldehyde and ethanol was largely delayed in coa ...200919191671
reconfiguration of the achene and receptacle metabolic networks during strawberry fruit development.the anatomy of strawberry (fragaria x ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. it can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in the process. by combining gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry with the aim of addressing the metabolic regulation underlying fruit seed ...200818715960
antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening.cell wall disassembly in softening fruits is a complex process involving the cumulative action of many families of wall-modifying proteins on interconnected polysaccharide matrices. one strategy to elucidate the in vivo substrates of specific enzymes and their relative importance and contribution to wall modification is to suppress their expression in transgenic fruit. it has been reported previously that inhibiting the expression of pectate lyase genes by antisense technology in strawberry (fra ...200818522930
functionality of bioactive compounds in brazilian strawberry (fragaria x ananassa duch.) cultivars: evaluation of hyperglycemia and hypertension potential using in vitro models.fruits of seven fully ripened strawberry cultivars grown in brazil (dover, camp dover, camarosa, sweet charlie, toyonoka, oso grande, and piedade) were evaluated for total phenolics, antioxidant activity based on dpph radical scavenging assay, and functionality such as inhibition of alpha-amylase, alpha-glucosidase, and angiotensin i-converting enzyme (ace) relevant for potentially managing hyperglycemia and hypertension. the total phenolics content ranged from 966 to 1571 microg of gallic acid/ ...200818522404
fermentation and malate metabolism in response to elevated co2 concentrations in two strawberry cultivars.concentrations of acetaldehyde, ethanol, ethyl acetate (ea), organic acids and activities and gene expression of alcohol dehydrogenase (adh; ec 1.1.1.1), pyruvate decarboxylase (pdc; ec 4.1.1.1), alcohol acyltransferase (aat; ec 1.4.1.14), malate dehydrogenase (mdh; ec 1.1.1.37), malic enzyme (me; ec 1.1.1.40) and glutamate dehydrogenase (ec 1.4.1.14) were investigated in two strawberry (fragaria x ananassa duch) cultivars with different responses to co(2) during storage. 'jewel' fruit treated w ...200818494736
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