| a new arabinofuranohydrolase from bifidobacterium adolescentis able to remove arabinosyl residues from double-substituted xylose units in arabinoxylan. | an arabinofuranohydrolase (axh-d3) was purified from a cell-free extract of bifidobacterium adolescentis dsm 20083. the enzyme had a molecular mass of approximately 100 kda as determined by gel filtration. it displayed maximum activity at ph 6 and 30 degrees c. using an arabinoxylan-derived oligosaccharide containing double-substituted xylopyranosyl residues established that the enzyme specifically released terminal arabinofuranosyl residues linked to c-3 of double-substituted xylopyranosyl resi ... | 1997 | 9114514 |
| effect of a viable yeast culture on digestibility and rumen fermentation in sheep fed different types of diets. | five mature wethers fitted with rumen fistulas were fed grass hay and a sugarbeet-pulp-based concentrate or maize silage and a cereal-based concentrate (50/50 digestible organic matter basis), or without with 5 g yeast supplement (saccharomyces cerevisiae, biosaf) per day in a latin square design. diets were given for a 28-d adaptation period, followed by a 10-d collection period to determine digestibility and nitrogen retention data. afterwards, rumen samples were taken on 3 consecutive days an ... | 1993 | 8447942 |
| arabinanase a from pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo- mode of action. | pseudomonas fluorescens subsp. cellulosa expressed arabinanase activity when grown on media supplemented with arabinan or arabinose. arabinanase activity was not induced by the inclusion of other plant structural polysaccharides, and was repressed by the addition of glucose. the majority of the pseudomonas arabinanase activity was extracellular. screening of a genomic library of p. fluorescens subsp. cellulosa dna constructed in lambda zapii, for recombinants that hydrolysed red-dyed arabinan, i ... | 1997 | 9163351 |
| the lactational responses of dairy cows to amount of feed and to the source of carbohydrate energy. | an experiment was designed to test whether responses to variation in plane of nutrition conformed to a linear model or to a diminishing response curve model and to examine the influence of type of energy-yielding nutrient used in the ration on the response. lactating friesian cows (n = 18; mean, 126 dim) were used in a latin square experiment with three 4-wk periods. diets consisted of hay and concentrates 40:60 (wt/wt, dm basis). the concentrates were based on grain, sugar beet pulp, or an equa ... | 1995 | 8786258 |
| influence of dietary factors on nitrogen partitioning and composition of urine and feces of fattening pigs. | an experiment was conducted to investigate the influence of dietary factors on the partitioning of nitrogen excretion and on the ph and composition of urine and feces of fattening pigs. sixteen male hybrid pigs of 80 to 90 kg bw were allotted to one of four diets: based on grains, by-products, grains plus tapioca, or grains plus sugar beet pulp. diets were formulated to have similar contents of ne and cp and a similar lysine:ne ratio. diets differed in nonstarch polysaccharide content (nsp) and ... | 1997 | 9078486 |
| new antifungal proteins from sugar beet (beta vulgaris l.) showing homology to non-specific lipid transfer proteins. | two novel, nearly identical antifungal proteins, iwf1 and iwf2, were isolated from the intercellular washing fluid (iwf) of sugar beet leaves. the proteins were purified to homogeneity and their amino acid sequences were determined. they are basic, monomeric proteins of 91 amino acid residues, 89 of which are identical. both proteins show strong in vitro antifungal activity against cercospora beticola, the casual agent of leaf spot disease in sugar beet. based on primary sequence homology, inclu ... | 1996 | 8790287 |
| the effects of physical form of feed, carbohydrate source, and inclusion of sodium bicarbonate on the diet selections of sheep. | we proposed an hypothesis that ruminants attempt to select a diet that promotes high levels of feed intake by maintaining optimal ruminal conditions. three tests of the hypothesis considered whether the diet selection of sheep given either a choice of two high energy density (ed) feeds or a choice between a high and a low ed feed is affected by 1) sodium bicarbonate inclusion (nahco3; 1, 2, and 4% [wt/wt]) in the high [ed] feed); 2) dietary carbohydrate source (barley-based, b and sugar beet/ ba ... | 1996 | 8791195 |
| single-chain fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay. | a recombinant enzyme-linked immunosorbent assay (elisa) system, entirely based on antibody fragments, is described here as an attractive alternative to assays using polyclonal antisera or monoclonal antibodies. two expression vectors have been developed for cloning and production of single-chain fv (scfv) fusion proteins suitable as coating and detecting reagents, respectively, in a highly sensitive double antibody sandwich elisa. the coating reagent is produced from the vector pzip1, as a bival ... | 1997 | 9212874 |
| identification of a bacterial pectin acetyl esterase in erwinia chrysanthemi 3937. | erwinia chrysanthemi causes soft-rot diseases of various plants by enzymatic degradation of the pectin in plant cell walls. the structural complexity of pectin requires the combined action of several pectinases for its efficient breakdown. three types of pectinases have so far been identified in e. chrysanthemi: two pectin methyl esterases (pema, pemb), a polygalacturonase (pehx), and eight pectate lyases (pela, pelb, pelc, peld, pele, pell, pelz, pelx). we report in this paper the analysis of a ... | 1997 | 9218776 |
| medicinal foodstuff. iii. sugar beet. (1): hypoglycemic oleanolic acid oligoglycosides, betavulgarosides i, ii, iii, and iv, from the root of beta vulgaris l. (chenopodiaceae). | betavulgarosides i, ii, iii, and iv, oleanolic acid oligoglycosides having an unique acidic substituent, were isolated from the root of beta vulgaris l. (sugar beet) together with betavulgarosides vi, vii, and viii. the chemical structures of betavulgarosides i, ii, iii, iv were identified from chemical and physicochemical evidence. betavulgarosides ii, iii, and iv were found to exhibit hypoglycemic activity in an oral glucose tolerance test in rats. | 1996 | 8814952 |
| improved delivery of biocontrol pseudomonas and their antifungal metabolites using alginate polymers. | alginate polymer was evaluated as a carrier for seed inoculation with a genetically modified strain pseudomonas fluorescens f113laczy, which protects sugar-beet against pythium-mediated damping-off. f113laczy survived in alginate beads at 5 log10 cfu/bead or higher counts for 8 weeks of storage, regardless of the conditions of incubation. in plant inoculation experiments, colonisation of the growing area of the root by f113laczy, derived from alginate beads placed in the soil next to the seed or ... | 1996 | 8867631 |
| energy metabolism in relation to physical activity in growing pigs as affected by type of dietary carbohydrate. | the effect of dietary carbohydrate source on physical activity in relation to metabolic rate in pigs was studied. six groups of 12 pigs (50-kg, castrated males) were fed one of two diets: a starch diet or a non-starch polysaccharide (nsp) diet. both diets had a similar calculated net energy content. the starch diet contained 13% tapioca, and the nsp diet contained 17% sugar beet pulp silage. pigs were housed in groups and fed at approximately two times maintenance (approximately 900 kj me.kg-.75 ... | 1996 | 8880425 |
| comparison of the influence of dietary fibre sources with different proportions of soluble and insoluble fibre on ca, mg, fe, zn, mn and cu apparent absorption in rats. | the 6 week experiment was performed on growing wistar male rats. in the third and the sixth weeks digestibility trials were performed. the following high fibre preparations were supplemented to the control diet (c): high methoxylated citrus pectin (cr); apple pomace (a), potato fiber "povex" (p) and sugar beet pulp (b). fibre supplementation, except cr, significantly increased ca, mg, fe, mn, zn, cu excretion in faeces and decreased their apparent absorption in both trials. significant correlati ... | 1996 | 8988315 |
| characterization of a new antifungal chitin-binding peptide from sugar beet leaves. | the intercellular washing fluid (iwf) from leaves of sugar beet (beta vulgaris l.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen cercospora beticola (sacc.). among these, a potent antifungal peptide, designated iwf4, was identified. the 30-amino-acid residue sequence of iwf4 is rich in cysteines (6) and glycines (7) and has a highly basic isoelectric point. iwf4 shows homology to the chitin-binding (hevein) domain of chitin-binding pr ... | 1997 | 9008390 |
| the faea genes from aspergillus niger and aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides. | we report the cloning and characterization of a gene encoding a ferulic acid esterase, faea, from aspergillus niger and aspergillus tubingensis. the a. niger and a. tubingensis genes have a high degree of sequence identity and contain one conserved intron. the gene product, faea, was overexpressed in wild-type a. tubingensis and a protease-deficient a. niger mutant. overexpression of both genes in wild-type a. tubingensis and an a. niger protease-deficient mutant showed that the a. tubingensis g ... | 1997 | 9406381 |
| effect of substitution of sugarbeet pulp for barley in diets for finishing rabbits on growth performance and on energy and nitrogen efficiency. | one hundred forty new zealand white x california weanling rabbits were used to study the influence of increasing dietary contents of digestible fiber replacing nonstructural carbohydrates on feed efficiency and growth performance. four diets, formulated by replacing sugarbeet pulp (sbp) for barley grain, and two slaughter weights (2 or 2.5 kg) were used in a 4 x 2 factorial arrangement. inclusion of 15% sbp decreased dietary energy and cp digestibilities by 6% (p < .001), but nonsignificant effe ... | 1993 | 8394306 |
| purification and properties of arfi, an alpha-l-arabinofuranosidase from cytophaga xylanolytica. | an alpha-l-arabinofuranosidase (alpha-l-arabinofuranoside arabinofuranohydrolase [ec 3.2.1.55]; referred to below as arfi) from cytophaga xylanolytica xm3 was purified 85-fold by anion-exchange and hydrophobic interaction column chromatography. the native enzyme had a pi of 6.1 and an apparent molecular mass of 160 to 210 kda, and it appeared to be a trimer or tetramer consisting of 56-kda subunits. with p-nitrophenyl-alpha-l-arabinofuranoside as the substrate, the enzyme exhibited a k(m) of 0.5 ... | 1998 | 9435061 |
| [the mutagenic action of radioactive contaminants of feed and food products on strains ta98 and ta100 of salmonella typhimurium]. | radionuclide-polluted slices of beef (2775 bq/kg), pork (274.8 bq/kg), potato (159.0-444.0 bq/kg), carrot, garden beet, kidney bean and sorrel (44.5-460.0 bq/kg) have been studied for their mutagenic effect on the tester strains ta98 and ta100 of salmonella typhimurium and this index as a rule correlates with the level of the slices pollution. on the other hand the slices of cabbage and samples of rye and wheat (46.0-460.0 bq/kg) as well as fish (2314.0 bq/kg) had no mutagenic effect on these st ... | 1996 | 9044702 |
| ecbi and ecbr: homologs of luxi and luxr affecting antibiotic and exoenzyme production by erwinia carotovora subsp. betavasculorum. | erwinia carotovora subsp. betavasculorum ecb168 causes vascular necrosis and root rot of sugar beet and produces an antibiotic(s) that is antagonistic against other erwinia spp. ecbi- mutants of ecb168, each containing a single transposon insertion in the ecbi gene (for erwinia carotovora subsp. betavasculorum inducer), do not produce detectable levels of extracellular protease or antibiotic(s), and express less pectate lyase activity and virulence than the wild-type strain. a plasmid containing ... | 1997 | 9476353 |
| sugar beet fiber in formula diet reduces postprandial blood glucose, serum insulin and serum hydroxyproline. | the object of the study was to investigate the effects of sugar beet fiber in formula diet on blood glucose, serum insulin and serum hydroxyproline in humans. | 1998 | 9505164 |
| production of gibberellic acid by aspergillus niger using some food industry wastes. | the production of gibberellic acid by aspergillus niger and the possibility of utilizing food industry waste and residues as the sources of carbon in media were investigated. media prepared from molasses, vinasse, whey, sugar-beet waste and fruit pomace were used and ga3 yields were found in concentrations 310, 273.14, 120, 73, 118.13 mg/l in such media, respectively. it was observed that food industry wastes can be used as cheap sources of carbon for gibberellic acid production by aspergillus n ... | 1996 | 9127484 |
| site directed chromosomal marking of a fluorescent pseudomonad isolated from the phytosphere of sugar beet; stability and potential for marker gene transfer. | a plasmid-free, non-pathogenic, ribosomal rna group 1 fluorescent pseudomonad, pseudomonas fluorescens sbw25, was selected from the microflora of sugar beet (beta vulgaris) and modified to contain constitutively expressed marker genes. by site directed homologous recombination a kx cassette [kanamycin resistance (kanr) and catechol 2,3 dioxygenase (xyle)] and a zy cassette [lactose utilization (laczy, beta-galactosidase, lactose permease)] were introduced at least 1 mbp apart on the 6.6 mbp bact ... | 1995 | 8564013 |
| transformation of acinetobacter sp. strain bd413 by transgenic sugar beet dna. | the ability of acinetobacter sp. strain bd413(pfg4 delta nptii) to take up and integrate transgenic plant dna based on homologous recombination was studied under optimized laboratory conditions. restoration of nptii, resulting in kanamycin-resistant transformants, was observed with plasmid dna, plant dna, and homogenates carrying the gene nptii. molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional dna. | 1998 | 9546192 |
| characterization of galactosidases from aspergillus niger: purification of a novel alpha-galactosidase activity. | an enzyme with beta-galactosidase activity and three proteins exhibiting alpha-galactosidase activity were purified from a culture filtrate of aspergillus niger grown on arabinoxylan. beta-galactosidase, optimally active at ph 4 and 60-65 degrees c, was active against p-nitrophenyl-beta-d-galactopyranoside, lactose, and pectic galactan. it was not able to release galactose from sugar beet pectin or lemon pectin. its action on pectic galactan was increased by the presence of beta-galactanase. the ... | 1998 | 9549105 |
| influence of dietary factors on the ph and ammonia emission of slurry from growing-finishing pigs. | we investigated the effects of dietary factors on the ph and the ammonia emission from slurry of growing-finishing pigs. sixteen male hybrid pigs (80 to 90 kg bw) were allotted to one of four diets based on barley-wheat, tapioca, barley-tapioca, and sugar beet pulp. diets were formulated to have similar ne and cp contents and a similar lysine:ne ratio. diets differed in nonstarch polysaccharide content (nsp) and dietary electrolyte balance (deb). urine and feces were daily collected quantitative ... | 1998 | 9581936 |
| sequence comparison and classification of beet luteovirus isolates. | three distinct sequence groups were found among partial nucleotide sequences of 38 isolates of beet western yellows virus (bwyv) and beet mild yellowing virus (bmyv) from europe, iran and the usa. the first group contains both sugar beet and oilseed rape specific isolates, and the differentiating characteristic linked to this host range specificity are 2 single base pair changes in a 1,200 nucleotide region of the genome. it is proposed that the european bwyv strains that can be transferred at l ... | 1995 | 8572940 |
| [a new metabolite with fungistatic and bacteriostatic activity, produced by strain l-30 of flavobacterium sp]. | flavocin, an agent with fungistatic and bacteriostatic activities, was isolated from the culture of flavobacterium sp. l-30. the data on the physico-chemical and biological properties of flavocin a, the major active component, are presented. the component was identified with metabolites of the genus flavobacterium and other genera of nonsporulating bacteria. flavocin was highly efficient in the treatment of various farm crops. the antagonistic effect of flavocin was observed under field conditio ... | 1996 | 9054320 |
| nutritional implications of pectins in chicks in relation to esterification and origin of pectins. | an experiment was conducted to examine the effect of feeding high-methylated (hm) and low-methylated (lm) pectin on performance and physiological characteristics in broiler chicks. two levels each (1.5 and 3%) of hm citrus pectin (hmc), lm citrus pectin (lmc), or hm sugar beet pectin (hms) were added to a semi-purified basal diet. the experiment was conducted in battery brooders, and chicks received the diets as pellets from 6 to 27 d of age. the inclusion of lmc or hms in the diet had little or ... | 1996 | 8893300 |
| oxidized ethyl linoleate induces mucosal hypertrophy of the large intestine and affects cecal fermentation of dietary fiber in rats. | oxidized ethyl linoleate (oel) was prepared by aeration at low temperature. peroxide value (pov, meq/kg lipid) of oel was 1400; the major oxidized compounds were 9-hydroperoxy-cis, trans- and 13-hydroperoxy-trans, cis-octadecadienoate. rats fed fiber-free or sugar-beet fiber (sbf, 100g/kg diet) diets were divided into three groups for each diet, and administered oel (high oel group), oel diluted with ethyl linoleate (low oel group, pov 700) and nonoxidized ethyl linoleate (el group) through gast ... | 1996 | 8613881 |
| purification and characterization of a novel esterase induced by growth of aspergillus niger on sugar-beet pulp. | an inducible esterase has been isolated from a liquid culture of aspergillus niger grown on sugar-beet pulp. the enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. the enzyme was not active on methyl sinapinate, a good substrate for ferulic acid esterase iii, which was purified previously from a. niger [faulds and williamson (1994) microbiology 140, 779-787]. with methyl caffeate as substrate the enzyme had temperat ... | 1996 | 8679110 |
| serological characterization of the 3'-proximal encoded proteins of beet yellows closterovirus. | the 3'-proximal open reading frames (orfs) of beet yellows closterovirus, california isolate (byv-ca), were sequenced and the expression of the corresponding proteins analyzed. the nucleotide sequence of orf 5 (coding for p24) was most conserved compared with orf 7 (coding for p20) and orf 8 (coding for p21) among the isolates analyzed. polyclonal antisera were produced to gst fusion proteins of p24, p20, and p21. accumulation of p24, cp, p20 and p21 was studied in infected tetragonia expansa pl ... | 1998 | 9722879 |
| release of ferulic acid from sugar-beet pulp by using arabinanase, arabinofuranosidase and an esterase from aspergillus niger. | aspergillus niger cinnamoyl esterase (cinnae) is shown to be active towards a wide range of feruloylated oligosaccharides derived from sugar-beet pulp (sbp). the esterase hydrolysed ferulic acid ester-linked to either c-2 of arabinose or c-6 of galactose residues, and demonstrated the highest activity towards the feruloylated arabinose trisaccharide. however, cinnae was able to release only 0.88% of total alkali-extractable ferulic acid from sbp in 24 h when acting alone. to determine whether ce ... | 1996 | 8679111 |
| digestion of feed amino acids in the rumen and small intestine of dairy cows measured with nylon-bag techniques. | the disappearance of total n, non-protein-n and amino acid-n after washing, rumen incubation and intestinal passage of sugarbeet pulp, maize-gluten feed, maize feed meal, palm kernel meal, soyabean hulls, soyabean meal, grass silage, maize silage and concentrate was measured in four dairy cows using nylon-bag techniques. disappearance of amino acid-n after washing varied between feedstuffs from 14 to 69% of feed amino acid-n, and was lower than disappearance of non-protein-n. for sugarbeet pulp, ... | 1997 | 9059232 |
| fermentation of raw poultry byproducts for animal nutrition. | in this study, the fermentation of raw, inedible poultry byproducts mixed with sugarbeet pulp and dextrose and inoculated with lactobacillus plantarum and(or) enteroccocus faecium resulted in a drop of ph in the byproducts to approximately 4.0 to 4.5 within 48 h. to keep the fermented product stable for a period of 21 d, the addition of > or = 3% (wt/wt) of a fermentable carbohydrate was necessary. with a high inoculation level of approximately 10(8) to 10(9) l. plantarum per gram, or with acidi ... | 1993 | 8407654 |
| effects of fertilizer on insecticides adsorption and biodegradation in crop soils. | recent organic fertilizer treatments (cow manure, pig slurry, composts, or green manure) simultaneously increase insecticide adsorption onto soil and the insecticide soil persistence, indicating a mechanism of slow release of insecticide into soil by the organic matter. this occurred in sugar beet crops with aldicarb, thiofanox and imidacloprid; also, in leek, cauliflower and brussels sprouts crops with chlorpyrifos and chlorfenvinphos. in contrast, organic fertilizer treatments applied once or ... | 1996 | 8687996 |
| in vitro fermentation by human faecal bacteria of total and purified dietary fibres from brown seaweeds. | the in vitro degradation of dietary fibre from three brown seaweeds (himanthalia elongata, laminaria digitata and undaria pinnatifida) was studied, using human faecal flora. two sets of fibre were tested: (1) total algal fibres extracted from the whole algae, mainly composed of alginates, and (2) purified fibres (sulphated fucans, na-alginates and laminarans) representative of those contained in the whole brown algae. mannuronate, one algal component, was also investigated. substrate disappearan ... | 1996 | 8785203 |
| effects of the type and level of dietary fibre supplements on nitrogen retention and excretion patterns. | the hypothesis was tested that fermentable dietary fibre (df) sources elevate faecal n excretion at the expense of urinary n without affecting n retention. df that substantially increase fermentation (pectin, sugarbeet and soya bran) or are poorly fermented (crystalline cellulose and maize bran) were fed as supplements to a basal df-free diet at three dose levels: 0, 50 and 100 g supplement/kg basal diet. the diets were fed to juvenile male wistar rats for 2 weeks before a 7 d period when faeces ... | 1996 | 8785218 |
| the impact of diets varying in carbohydrates resistant to endogenous enzymes and lignin on populations of ascaris suum and oesophagostomum dentatum in pigs. | the impact of diets varying in type and level of carbohydrates resistant to endogenous enzymes and lignin on the establishment and location of ascaris suum and oesophagostomum dentatum was investigated experimentally. fifty worm-free pigs, from a specific pathogen-free farm were used. the animals were assigned randomly to 5 diets and infected with 600 infective a. suum eggs and 6000 infective larvae of o. dentatum per pig. the diets consisted of a traditional ground barley plus protein feed (die ... | 1997 | 9172427 |
| influence of particle size on the effectiveness of beet pulp fiber. | sixteen holstein cows in midlactation were used in a design based on a replicated 4 x 4 latin square with the last period removed to determine the influence of particle size of beet pulp neutral detergent fiber (ndf) on its effectiveness as a replacement for alfalfa ndf. diets were a low forage, low fiber diet [12.1 g of alfalfa ndf/100 g of dry matter (dm)], a normal forage diet (low forage plus 7.8 g of additional alfalfa ndf/100 g of dm), and two low forage diets with 5.3 g of ndf/100 g of dm ... | 1997 | 9178130 |
| genetic characterization of populations of a de novo arisen sugar beet pest, aubeonymus mariaefranciscae (coleoptera, curculionidae), by rapd analysis. | the weevil aubeonymus mariaefranciscae is an important pest of sugar beet crops in southern spain that was first described as a new species in 1979. we have studied the genetic variability of this insect by rapd analysis of 122 individuals using eight primers. the high resolution provided by random amplified polymorphic dna (rapds), in combination with efficient genetic distance estimators, allowed a very detailed description of the ecology and evolution of the populations of this insect in the ... | 1997 | 9211731 |
| detection of alien chromatin conferring resistance to the beet cyst nematode (heterodera schachtii schm.) in cultivated beet (beta vulgaris l.) using in situ hybridization. | chromatin originating from wild beets of the genus beta, section procumbentes, has been investigated in nematode-resistant hybrid-derived lines of sugar beet (beta vulgaris l.) by in situ hybridization using satellite, telomeric and ribosomal dna repeats, a yeast artificial chromosome (yac) and total genomic dna as probes. the allen chromosome was detected in three monosomic addition lines (2n = 18 + 1) by genomic in situ hybridization. fluorescence in situ hybridization with a genome-specific s ... | 1997 | 9246412 |
| characterization of six bacteriophages of serratia liquefaciens cp6 isolated from the sugar beet phytosphere | six phages (phicp6-1 to phicp6-6) that are commonly found in the phytosphere of sugar beet (beta vulgaris var. amethyst) were investigated, and their relative impacts on their host (serratia liquefaciens cp6) were compared. there were fundamental differences between the two most abundant predators of cp6 (phicp6-1 and phicp6-4). like phicp6-2 and phicp6-5, phicp6-1 belonged to the family siphoviridae, while phicp6-4 exhibited the morphology of the family podoviridae. the other phages were member ... | 1999 | 10223986 |
| detection and characterization of a distinct type of beet necrotic yellow vein virus rna 5 in a sugarbeet growing area in europe. | a fifth beet necrotic yellow vein virus (bnyvv) rna species has been detected in europe in sugarbeet infected with p-type bnyvv. very little sequence variation was found between two european sources of this rna 5*, but considerable differences were detected between these two european sources on the one hand and the four japanese sources recently analysed by kiguchi et al. on the other. the bnyvv rna 5-encoded 26 k proteins share a stretch of six amino acids (frgpgn) with the bnyvv rna 3-encoded ... | 1997 | 9267459 |
| [investigations in central hessia on the occurrence of estrogenic activity content of cattle feed]. | in 21 farms which requested consultation for reasons of infertility problems feed samples (grass silage, n = 20; cornsilage, n = 19; hay, n = 9; cereal mixture, n = 3, brewers grains, n = 5, ensiled sugar beet tops, n = 1) were drawn for assay of free (aglycon) and glycosidic (glycosid) bound oestrogen activity. following sample preparation and extraction, the oestrogenic activity was determined using a radioreceptorassay and only those samples leading to a 50% displacement of the 3h-estradiol-1 ... | 1997 | 9324454 |
| evidence for signaling between the phytopathogenic fungus pythium ultimum and pseudomonas fluorescens f113: p. ultimum represses the expression of genes in p. fluorescens f113, resulting in altered ecological fitness. | there is increasing evidence that communication between members of the same species, as well as members of different species, is important for the survival of microorganisms in diverse ecological niches, such as the rhizosphere. to investigate whether the phytopathogen pythium ultimum could alter gene expression in the biocontrol strain pseudomonas fluorescens f113, which protects the roots of sugar beet from the fungus, a screening system was developed to detect differential expression of bacte ... | 1997 | 9361412 |
| influence of a blend of fructo-oligosaccharides and sugar beet fiber on nutrient digestibility and plasma metabolite concentrations in healthy beagles. | to evaluate effects of a blend of fructo-oligosaccharides and sugar beet fiber (4:1) at 3 incorporation rates on nutrient digestibility and plasma glucose, insulin, alpha-aminonitrogen, urea, cholesterol, and triglycerides concentrations measured weekly in nonfed dogs and during a 360-minute period after a meal. | 1997 | 9361885 |
| role of the 14-3-3 proteins in the regulation of h+-atpase activity in the plasma membrane of suspension-cultured sugar beet cells under cold stress. | all higher plants possess highly specific binding sites for fusicoccin, a metabolite of the fungus fusicoccum amygdali del. these sites are harboured in the plasma membranes and formed by a 14-3-3 protein dimer associated with the c-terminal autoinhibitory domain of h+-atpase. we considered the fusicoccin binding to plasma membranes to be an indicator of complexation between the 14-3-3 dimer and h+-atpase, we assessed the effect of cold stress on the interaction of these proteins in suspension-c ... | 1999 | 10452522 |
| possible role of xanthobaccins produced by stenotrophomonas sp. strain sb-k88 in suppression of sugar beet damping-off disease. | three antifungal compounds, designated xanthobaccins a, b, and c, were isolated from the culture fluid of stenotrophomonas sp. strain sb-k88, a rhizobacterium of sugar beet that suppresses damping-off disease. production of xanthobaccin a in culture media was compared with the disease suppression activities of strain sb-k88 and less suppressive strains that were obtained by subculturing. strain sb-k88 was applied to sugar beet seeds, and production of xanthobaccin a in the rhizosphere of seedlin ... | 1999 | 10508056 |
| cutting, ageing and expression of plant membrane transporters. | the activity and the expression of sucrose, hexose and amino acid transporters were studied with fresh, cut or aged tissues and plasma membrane vesicles (pmv) of mature sugar beet (beta vulgaris l.) leaves. cutting and ageing both induced an increase of the transcripts coding for sucrose transporters and hexose transporters. no significant effect could be detected on the amino acid transporter transcripts with the probe used (aap1). a polyclonal serum directed against the arabidopsis thaliana su ... | 1997 | 9408174 |
| suppression of the number of aberrant crypt foci of rat colorectum by ingestion of sugar beet fiber regardless of administration of anti-asialo gm1. | anti-asialo gm1 serum (agm1) reduces natural killer (nk) activity in vitro and in vivo. we investigated the effect of ingestion of sugar beet fiber (sbf) on 1,2-dimethylhydrazine (dmh)-induced aberrant crypt foci (acf) and whether the effect was maintained under nk-reducing conditions by agm1 injection. the ingestion of sbf decreased the number of acf in the colorectum at 4 weeks after treatment with dmh. dietary sbf had a suppressive effect on the formation of acf regardless of the administrati ... | 1997 | 9459172 |
| effect of inclusion of sugar beet pulp, pelleting and season on laying hen performance. | 1. the effect of three metabolisable energy (me) concentrations 12.2, 11.3 and 10.5 mj me/kg and two forms of diet (mash and pellet) on egg production in summer and winter were studied. the different me values were obtained by diluting the 12.2 mj me/kg diet with sugar beet pulp of 2.88 mj/kg dm. in a 3rd summer experiment the water intake of hens on these diets was examined. data were analysed by factorial analysis of variance, and the relationship between performance and dietary energy concent ... | 1997 | 9510998 |
| fermentation products of sugar-beet fiber by cecal bacteria lower plasma cholesterol concentration in rats. | plasma cholesterol concentration is reduced by feeding some dietary fibers but the mechanism is not fully understood. we examined whether cecal fermentation products are involved in lowering plasma cholesterol by feeding rats a highly fermentable sugar-beet fiber (sbf) in four separate experiments. these were designed to investigate the effects on plasma cholesterol of oral ingestion of fermentation products on plasma cholesterol, the effects of the products in comparison with that of a short-ch ... | 1998 | 9521629 |
| an alpha-l-arabinofuranosidase from penicillium purpurogenum: production, purification and properties. | penicillium purpurogenum secretes arabinofuranosidase to the growth medium. highest levels of enzyme (1.0 u ml(-1)) are obtained when l-arabitol is used as carbon source, while 0.85 and 0.7 u ml(-1) are produced with sugar beet pulp and oat spelts xylan, respectively. by means of a zymogram, three bands with arabinofuranosidase activity have been detected in the supernatant of a culture grown in oat spelts xylan. one of the enzymes was purified to homogeneity from this supernatant using gel filt ... | 2000 | 10656340 |
| effect of adding sugar beet fibre and wheat bran to a starch diet on the absorption kinetics of glucose, amino-nitrogen and volatile fatty acids in the pig. | the purpose of this study with the pig was to analyse the influence of the type of dietary fibre on quantitative kinetics of the absorption of nutrients deriving from enzymatic digestion in the small intestine and that of volatile fatty acids (vfa) deriving from microbial digestion in the hindgut influenced by the length of adaptation to the diet. two groups of four pigs were fitted with a device for measuring absorption by simultaneous analysis of the differences in the porto-arterial concentra ... | 1998 | 9606749 |
| the influence of sugar-beet fibre, guar gum and inulin on nutrient digestibility, water consumption and plasma metabolites in healthy beagle dogs. | the aim of the present study was to evaluate the effects of three fibres (sugar-beet fibre, guar gum and inulin) incorporated in the basal diet of healthy dogs at 7 per cent of dry matter (dm). parameters examined included stool output, water consumption, nutrient digestibility and fasting and postprandial plasma metabolites. all fibres increased wet faecal output; an increase in faecal dm output being observed with sugar-beet fibre only. sugar-beet fibre and inulin increased daily water consump ... | 1998 | 9625462 |
| improved detection of beet necrotic yellow vein virus in a das elisa by means of antibody single chain fragments (scfv) which were selected to protease-stable epitopes from phage display libraries. | the detection of beet necrotic yellow vein virus (bnyvv) in stored sugar beets by means of monoclonal antibodies or antibody single chain fragments (scfv) often poses problems, because the immunodominant c-terminal epitope of the viral coat protein is readily lost due to proteolysis. clones which produce scfv specific for protease-stable bnyvv epitopes were selected from two naive phage display libraries. fusion proteins of the scfv with a human igg kappa chain (expressed from the newly designed ... | 2000 | 10664416 |
| transformation of acinetobacter sp. strain bd413(pfg4deltanptii) with transgenic plant dna in soil microcosms and effects of kanamycin on selection of transformants. | here we show that horizontal transfer of dna, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. restoration of a 317-bp-deleted nptii gene in acinetobacter sp. strain bd413(pfg4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant dna encoding a functional nptii gene conferring bacterial kanamycin resistance. selective effects of the addition of kanamycin on the population dynamics of aci ... | 2000 | 10698801 |
| from sugar beet molasses to lyphan. integrated quality management from the raw material to the drug. | l-tryptophan is produced at the amino gmbh (frellstedt, frg) via biocatalytical condensation of the amino acid l-serine with indole. as a biocatalyst, tryptophan synthetase is used which is produced in high activities by a natural mutant escherichia coli strain. the enzyme mechanism and specificity and the individual process-parameters for the biotransformation procedure are explained as well as the purification process of educts and products. this includes a detailed description of the quality ... | 1999 | 10721087 |
| generation of monoclonal antibody specific to (1-->5)-alpha-l-arabinan. | a neoglycoprotein (a heptasaccharide of (1-->5)-alpha-l-linked-arabinosyl residues linked to bovine serum albumin) has been used to generate a rat monoclonal antibody specific to a linear chain of (1-->5)-alpha-l-arabinan which is a structural feature of the side chains of pectins. the antibody, designated lm6, detected 100 ng of debranched sugar beet arabinan in an immunodot binding assay and 1 microgram of commercial citrus pectin in a similar assay. hapten inhibition studies indicated that th ... | 1998 | 9675359 |
| dietary carbohydrates alter the fecal composition and ph and the ammonia emission from slurry of growing pigs. | we investigated the effects of dietary carbohydrates on the composition and ph of fecal material and on the ammonia emission from the slurry of growing pigs. thirty-four barrows (bw approximately 40 kg) were randomly allotted to 1 of 10 diets. a basal diet was formulated to meet all requirements for protein, amino acids, minerals, and vitamins. the control diet was composed of the basal diet plus heat-treated cornstarch. in the other diets, the cornstarch in the control diet was replaced with th ... | 1998 | 9690645 |
| genome properties of beet virus q, a new furo-like virus from sugarbeet, determined from unpurified virus. | based solely on the information that beet virus q (bvq) contains tubular particles, the entire nucleotide sequence of its tripartite genome was determined from unpurified virus in ca. 40 ml crude sap from locally infected chenopodium quinoa. a starting sequence for rna 1 was generated using primers corresponding to highly conserved helicase domains in the respective rnas of furo-, pomo-, peclu-, hordei- and tobraviruses, and was extended by a walking random-primed cdna approach. the similarity o ... | 1998 | 9714254 |
| dietary modulation of avian coccidiosis. | during the past several years, our laboratory has been investigating the anticoccidial activities of various natural products that have potential use as dietary supplements for coccidiosis control. sources of fats containing high concentrations of n-3 fatty acids such as menhaden oil and flaxseed oil and flaxseed, when added to starter rations and fed to chicks from one day of age, effectively reduce lesions caused by the caecal parasite eimeria tenella, but not lesions caused by eimeria maxima. ... | 1998 | 9724884 |
| secondary metabolite- and endochitinase-dependent antagonism toward plant-pathogenic microfungi of pseudomonas fluorescens isolates from sugar beet rhizosphere | forty-seven isolates representing all biovars of pseudomonas fluorescens (biovars i to vi) were collected from the rhizosphere of field-grown sugar beet plants to select candidate strains for biological control of preemergence damping-off disease. the isolates were tested for in vitro antagonism toward the plant-pathogenic microfungi pythium ultimum and rhizoctonia solani in three different plate test media. mechanisms of fungal inhibition were elucidated by tracing secondary-metabolite producti ... | 1998 | 9758768 |
| effect of carbon source on production of alpha-l-arabinofuranosidase by aureobasidium pullulans | a color-variant strain of aureobasidium pullulans (nrrl y-12974) produced alpha-l-arabinofuranosidase (alpha-l-afase) when grown in liquid culture on sugar beet arabinan, wheat arabinoxylan, l-arabinose, l-arabitol, xylose, xylitol, oat spelt xylan, corn fiber, or arabinogalactan. l-arabinose was most effective for production of both whole-broth and extracellular alpha-l-afase activity, followed by l-arabitol. oat spelt xylan, sugar beet arabinan, xylose, xylitol, and wheat arabinoxylan were int ... | 1998 | 9767714 |
| in situ population dynamics of bacterial viruses in a terrestrial environment | predation by bacteriophages is thought to control bacterial numbers and facilitate gene transfer among bacteria in the biosphere. a thorough understanding of phage population dynamics is therefore necessary if their significance in natural environments is to be fully appreciated. here we describe the in situ population dynamics of three separate phage populations predating on separate bacterial species, living on the surface of field-grown sugar beet (beta vulgaris var. amethyst), as recorded ov ... | 1999 | 9872776 |
| growth conditions of aspergillus sp. athum-3482 for polygalacturonase production. | a wild type of aspergillus sp. athum-3482 produced extracellular polygalacturonase when grown in liquid medium containing citrus pectin as sole carbon source. a number of factors affecting enzyme activity were investigated. polygalacturonase activities as high as 4.3 u ml-1 (reducing-group-releasing activity) and 17 u ml-1 (viscosity-diminishing activity) were obtained under optimum growth conditions. with sugar-beet as sole carbon source the respective activities were 6.5 u ml-1 and 40 u ml-1, ... | 1997 | 9163956 |
| evaluation of the physical structure of fodder beets, potatoes, pressed beet pulp, brewers grains, and corn cob silage. | the physical structure of fodder beets, raw potatoes, ensiled pressed sugar beet pulp, ensiled brewers grains, and corn cob silage was evaluated in diets containing concentrates and either corn silage or grass silage as the roughage source. in one series of experiments, the chewing activity of eight cows was measured over 4 d. the beet pulp and fodder beets were added to the two roughage sources at two ratios [20:80 and 35:65, dry matter (dm) basis]. potatoes, brewers grains, and corn cob silage ... | 1999 | 10022013 |
| cancer mortality in agricultural regions of minnesota. | because of its unique geology, minnesota can be divided into four agricultural regions: south-central region one (corn, soybeans); west-central region two (wheat, corn, soybeans); northwest region three (wheat, sugar beets, potatoes); and northeast region four (forested and urban in character). cancer mortality (1980-1989) in agricultural regions one, two, and three was compared to region four. using data compiled by the national center for health statistics, cancer mortality was summarized by 5 ... | 1999 | 10064550 |
| short-chain fatty acids suppress cholesterol synthesis in rat liver and intestine. | we previously showed that plasma cholesterol levels decreased following ingestion of a short-chain fatty acid (scfa) mixture composed of sodium salts of acetic, propionic, and butyric acids simulating cecal fermentation products of sugar-beet fiber (sbf). in the present study, we investigated whether hepatic and small intestinal cholesterol synthesis is involved in the cholesterol-lowering effects of scfa and sbf. in vitro (expt. 1) and in vivo (expt. 2) cholesterol synthesis rates and the diurn ... | 1999 | 10222383 |
| feruloyl esterase from aspergillus sp.: purification, properties, and action on natural substrates | an enzyme active toward the methyl ester of ferulic acid was isolated from the fungus aspergillus sp. and purified to homogeneity using ion-exchange and hydrophobic chromatography. the molecular weight of the enzyme is 42 kd, and its pi is 3.7. the enzyme has a ph optimum in the range 4-6 and a temperature optimum in the range 40 to 60 degrees c. using a number of synthetic and natural substrates, the enzyme was identified as a feruloyl esterase. the feruloyl esterase did not hydrolyze wheat str ... | 1999 | 10231593 |
| effect of rumen protected methionine supplementation on early lactational responses of dairy cows fed a grass silage and cereals diet. | to investigate the milk production limiting potential of a diet based on grass silage (40%), hay (15%), dried sugar beet pulp (13%) and grain compound mixture (32%), 28 multiparous cows in early lactation were randomly assigned to two treatment groups: a control group and a group receiving supplementary rumen protected methionine (rpmet) treatment (12 g intestinally available methionine/cow/day, given 1-120 days postpartum; smartamine; rpan's technology). the diet was formulated to meet the requ ... | 1999 | 10344080 |
| ingestion of sugar beet fiber enhances irradiation-induced aberrant crypt foci in the rat colon under an apoptosis-suppressed condition. | the induction of aberrant crypt foci (acf) by irradiation of gamma-rays (60co), and the effect of dietary sugar beet fiber (sbf) on irradiation-induced acf were examined. we found that abdominal irradiation of gamma-rays could induce acf in the rat colon. the irradiation was performed once a week at a dose rate of 2 or 3 gy per irradiation. irradiation-induced acf were observed in the colon at 10 weeks after the first irradiation at dose of 2 gy for six times or 3 gy for four times. dietary sbf ... | 1999 | 10357780 |
| processing, disulfide pattern, and biological activity of a sugar beet defensin, ax2, expressed in pichia pastoris. | ax2 is a 46-amino-acid cysteine-rich peptide isolated from sugar beet leaves infected with the fungus cercospora beticola (sacc.). ax2 strongly inhibits the growth of c. beticola and other filamentous fungi, but has little or no effect against bacteria. ax2 is produced in very low amounts in sugar beet leaves, and to study the protein in greater detail with respect to biological function and protein structural analysis, the methylotrophic yeast pichia pastoris was used for large-scale production ... | 1999 | 10425158 |
| chitinolytic activity of pseudomonas fluorescens isolates from barley and sugar beet rhizosphere. | twelve isolates of pseudomonas fluorescens, isolated from barley and sugar beet rhizosphere as antagonists towards the plant pathogenic microfungi rhizoctonia solani and pythium ultimum, showed chitinolytic activity in batch cultures when grown in media without exogenous chitin. enzyme tests in cultures demonstrated a complete array of chitinolytic enzymes. endochitinase and chitobiosidase activities seemed to be tightly coupled in the isolates, while there was no relationship to n-acetyl-glucos ... | 1999 | 10525178 |
| ecological basis for biocontrol of damping-off disease by pseudomonas fluorescens 54/96 | pseudomonas fluorescens 54/96, originally isolated from the rhizosphere of sugar beet, has been shown to be commercially effective in field trials for the suppression of a number of fungal diseases of seedlings. in vitro and microcosm-based assays revealed that both the timing and method of application of bacteria were important for effective control of pythium ultimum, the causative agent of damping-off disease. following transposon mutagenesis (tn5lac), mutants deficient for the suppression of ... | 1999 | 10540249 |
| microbacterium kitamiense sp. nov., a new polysaccharide-producing bacterium isolated from the wastewater of a sugar-beet factory. | two strictly aerobic, heterotrophic and mesophilic new organisms, strains kitami a1 and kitami c2t, were isolated from the wastewater of a sugar-beet factory in kitami city, hokkaido, japan. in batch cultures, these organisms produced both insoluble and soluble exopolysaccharides (epss) utilizing sucrose as the sole carbon source. the g + c contents of the strains kitami c2t and kitami a1 were 69.2 mol%. both strains had anteiso-c15:0 acid, anteiso-c17:0 acid and iso-c16:0 as major components. t ... | 1999 | 10555312 |
| a modular esterase from penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module. | an esterase was isolated from cultures of the filamentous fungus penicillium funiculosum grown on sugar beet pulp as the sole carbon source. the enzyme (ferulic acid esterase b, faeb) was shown to be a cinnamoyl esterase (ce), efficiently releasing hydroxycinnamic acids from synthetic ester substrates and plant cell walls, and bound strongly to microcrystalline cellulose. a gene fragment was obtained by pcr using partial amino-acid sequences obtained from the pure enzyme and used to a probe a p. ... | 2000 | 11082184 |
| overproduction of spinach betaine aldehyde dehydrogenase in escherichia coli. structural and functional properties of wild-type, mutants and e. coli enzymes. | betaine aldehyde dehydrogenase (badh) catalyzes the last step in the synthesis of the osmoprotectant glycine betaine from choline. although betaine aldehyde has been thought to be a specific substrate for badh, recent studies have shown that human and sugar beet badhs also catalyze the oxidation of omega-aminoaldehydes. to characterize the kinetic and stability properties of spinach badh, five kinds of expression vectors encoding full length, mature, e103q, e103k, and chimera badhs were construc ... | 2000 | 11106411 |
| efficient expression of foreign proteins in roots from tobravirus vectors. | viral vectors were constructed from infectious cdna clones of each of the three tobraviruses, tobacco rattle virus (trv), pea early-browning virus (pebv), and pepper ringspot virus (peprsv). rna2 of each of the three viruses was modified to carry an additional coat protein subgenomic promoter and was used to express green fluorescent protein (gfp) when inoculated to plants. the tobravirus expression vectors have a wide host range and were able to express gfp in, for example, nicotiana species, t ... | 2000 | 10648180 |
| identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes. | isoflavones have drawn much attention because of their benefits to human health. these compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid pathway. to identify the gene encoding this enzyme, we used a yeast expression assay to screen soybean ests encoding cytochrome p450 proteins. we identified two soybean genes encod ... | 2000 | 10657130 |
| beticolins, nonpeptidic, polycyclic molecules produced by the phytopathogenic fungus cercospora beticola, as a new family of ion channel-forming toxins. | beticolins are toxins produced by cercospora beticola, a phytopathogenic fungus responsible for the leaf spot disease of sugar beet. they form a family of 20 nonpeptidic compounds (named b0 to b19) that share the same polycyclic skeleton but differ by isomeric configuration (ortho- or para-) and by a variable residue r (bridging two carbons in one of the six cycles). it has been previously shown that b0 assembles itself into a multimeric structure and forms ion channels into planar lipid bilayer ... | 2000 | 10659710 |
| monitoring gene flow from transgenic sugar beet using cytoplasmic male-sterile bait plants. | one of the most discussed environmental effects associated with the use of transgenic plants is the flow of genes to plants in the environment. the flow of genes may occur through pollen since it is the reproductive system that is designed for gene movement. pollen-mediated gene escape is hard to control in mating plants. pollen from a wind pollinator can move over distances of more than 1000 m. to investigate the efficiency of transgenic pollen movement under realistic environmental conditions, ... | 2000 | 11123616 |
| effect of supplementing a fibre basal diet with different nitrogen forms on ruminal fermentation and microbial growth in an in vitro semi-continuous culture system (rusitec) | incubation trials were carried out with the rumen simulation technique (rusitec) to study the effects of four forms of n on the growth of ruminal micro-organisms and the fermentation variables when an all-fibre basal diet was incubated. the basal diet consisted of 10 g neutral-detergent fibre (ndf) from grass hay plus 2 g ndf from sugarbeet pulp. n forms were isolated soyabean protein, soyabean peptides, amino acids blended to profile soyabean protein and nh3 as nh4cl. half of the daily n supply ... | 1999 | 10743487 |
| characterization of a new antifungal non-specific lipid transfer protein (nsltp) from sugar beet leaves. | a novel protein (iwf5) comprising 92 amino acids has been purified from the intercellular washing fluid of sugar beet leaves using cation exchange chromatography and reversed phase high performance liquid chromatography. based on amino acid sequence homology, including the presence of eight cysteines at conserved positions, the protein can be classified as a member of the plant family of non-specific lipid transfer proteins (nsltps). the protein is 47% identical to iwf1, an antifungal nsltp prev ... | 2000 | 10773337 |
| fermentation of plant cell wall derived polysaccharides and their corresponding oligosaccharides by intestinal bacteria. | new types of nondigestible oligosaccharides were produced from plant cell wall polysaccharides, and the fermentation of these oligosaccharides and their parental polysaccharides by relevant individual intestinal species of bacteria was studied. oligosaccharides were produced from soy arabinogalactan, sugar beet arabinan, wheat flour arabinoxylan, polygalacturonan, and rhamnogalacturonan fraction from apple. all of the tested substrates were fermented to some extent by one or more of the individu ... | 2000 | 10820072 |
| identification of conserved traits in fluorescent pseudomonads with antifungal activity. | a collection of 29 fluorescent pseudomonads, some with known biological control activity against a range of phytopathogenic fungi, were characterized phenotypically and genotypically by comparing carbon source utilization patterns, suppression of pythium ultimum both in planta and in vitro and the potential to produce known secondary metabolites. fatty acid profiling and restriction fragment length polymorphism (rflp) analysis of the ribosomal dna operon (ribotyping) were used to determine the d ... | 2000 | 11200428 |
| fermentations of pectin-rich biomass with recombinant bacteria to produce fuel ethanol. | pectin-rich residues from sugar beet processing contain significant carbohydrates and insignificant amounts of lignin. beet pulp was evaluated for conversion to ethanol using recombinant bacteria as biocatalysts. hydrolysis of pectin-rich residues followed by ethanolic fermentations by yeasts has not been productive because galacturonic acid and arabinose are not fermentable to ethanol by these organisms. the three recombinant bacteria evaluated in this study, escherichia coli strain ko11, klebs ... | 2000 | 10849785 |
| dietary sugar beet fiber prevents the increase in aberrant crypt foci induced by gamma-irradiation in the colorectum of rats treated with an immunosuppressant. | we demonstrated recently that gamma-irradiation can induce aberrant crypt foci (acf) in the rat colorectum. the aim of this study was to evaluate the effect of dietary sugar beet fiber (sbf) on the distribution of the cd8(+) intraepithelial lymphocyte (iel) in the colorectum and on the number of gamma-irradiation-induced acf of rats administered anti-asialo gm1 (alpha agm1) as an immunosuppressant. wistar/st rats fed a fiber-free diet or the diet supplemented with sbf (100 g/kg diet) were admini ... | 2000 | 10867036 |
| simultaneous detection of the establishment of seed-inoculated pseudomonas fluorescens strain dr54 and native soil bacteria on sugar beet root surfaces using fluorescence antibody and in situ hybridization techniques. | colonization at sugar beet root surfaces by seedling-inoculated biocontrol strain pseudomonas fluorescens dr54 and native soil bacteria was followed over a period of 3 weeks using a combination of immunofluorescence (dr54-targeting specific antibody) and fluorescence in situ hybridization (rrna-targeting eubacteria eub338 probe) techniques with confocal laser scanning microscopy. the dual staining protocol allowed cellular activity (ribosomal number) to be recorded in both single cells and micro ... | 2000 | 10922498 |
| overproduction of an inducible extracellular serine protease improves biological control of pythium ultimum by stenotrophomonas maltophilia strain w81. | stenotrophomonas maltophilia w81 can protect sugar beet against pythium:-mediated damping-off disease through the production of an extracellular protease. here, the proteolytic enzyme of w81 was purified by anion-exchange chromatography and characterized as a serine protease. the purified enzyme was fungicidal against pythium: ultimum in vitro. its synthesis was inducible by casein in w81, and mutagenesis of this strain using the luciferase (luxab) reporter transposon tn5-764cd resulted in the i ... | 2000 | 10931911 |
| net energy value of non-starch polysaccharide isolates (sugarbeet fibre and commercial inulin) and their impact on nutrient digestive utilization in healthy human subjects. | the energy value of nsp has been expressed as their metabolizable energy (me) content. the aim of the present study was to determine whether differences in me and net energy (ne) contents were similar for insoluble and soluble nsp. nine healthy young men were offered three diets according to a latin-square design (3 x 3) with three repetitions: diet c (control), diet b (control + 50 g sugarbeet fibre/d) and diet i (control + 50 g commercial inulin/d). after a 16 d adaptation period to nsp isolat ... | 1998 | 9924276 |
| exopolysaccharide-producing bacteria from sugar beets. | six hundred microorganisms were isolated from sugar beets collected from different parts of finland to study their slime production. a total of 170 of them produced exopolysaccharides, of which 35% were heteropolysaccharides. the yield of heteropolysaccharides from sucrose was lower than that of dextrans. five isolates, which were chosen for closer study, were identified as leuconostoc mesenteroides (two species), rahnella aquatilis (two species), and enterobacter amnigenus. | 1999 | 9925632 |
| [cloning and characterization of cmo gene from atriplex hortensis]. | glycine betaine is a widespread osmopretectant existed in many organisms. in higher plant, glycine betaine is synthesized via a two-sep oxidation reaction: choline-->betaine aldehyde-->glycine betaine. the first step, also the speed-limiting step, is catalyzed by choline monooxygenase(cmo). choosing halophyte atriplex hortensis as material, we constructed a salt stress-induced cdna library, and isolated a 1.77 kb length cdna clone with spinach cmo cdna as probe. the sequencing result showed a co ... | 2001 | 11330176 |
| the energetic value of nonstarch polysaccharides in relation to physical activity in group-housed, growing pigs. | the dose response effect of dietary nonstarch polysaccharide (nsp) content on physical activity in relation to metabolic rate in pigs was studied. twelve clusters of 14 pigs (50-kg castrated males) were fed one of four diets, similar in composition, except for the starch and nsp content. the difference in starch and nsp ratio was created by exchanging sugar beet pulp silage (sbps) for tapioca. on a dm basis, diets contained 0, 5, 10, or 15% sbps. pigs were housed in groups and fed at 2.5 times t ... | 1998 | 9928606 |
| dietary sugar beet fiber ameliorates diarrhea as an acute gamma-radiation injury in rats. | gamma radiation induces diarrhea as an acute injury. we have studied whether ingestion of sugar beet fiber influences radiation-induced diarrhea. abdominal irradiation with gamma rays induced diarrhea in male wistar/st rats from 2 to 7 days after a single sublethal dose. the body weight of the irradiated rats was decreased temporarily at 4 days after irradiation regardless of the ingestion of sugar beet fiber. at day 8, it returned to almost the same level as that of unirradiated rats. a change ... | 2000 | 10956431 |
| hepatic ldl receptor mrna in rats is increased by dietary mushroom (agaricus bisporus) fiber and sugar beet fiber. | plasma cholesterol concentration is reduced by feeding some dietary fibers and mushroom fruit body, but the mechanism is not fully understood. we examined the effects of mushroom (agaricus bisporus) fiber and sugar beet fiber on serum cholesterol and hepatic ldl receptor mrna in rats. rats were fed a cholesterol-free diet with 50 g/kg cellulose powder (cp), 50 g/kg mushroom (agaricus bisporus) fiber (msf) or 50 g/kg sugar beet fiber (bf) for 4 wk. there were no significant differences in the bod ... | 2000 | 10958806 |
| viscosinamide-producing pseudomonas fluorescens dr54 exerts a biocontrol effect on pythium ultimum in sugar beet rhizosphere. | growth inhibition of the root pathogen pythium ultimum by the biocontrol agent pseudomonas fluorescens dr54 inoculated on sugar beet seeds was studied in a soil microcosm. plant emergence was followed, together with bacterial rhizosphere colonization, antibiotic production and effects on fungal growth. p. fluorescens dr54 inoculation of the p. ultimum-challenged seeds improved plant emergence after 7 days compared to a control without the biocontrol strain. at this time, p. fluorescens dr54 was ... | 2000 | 10967213 |
| use of cc traps with different trap base colors for silverleaf whiteflies (homoptera: aleyrodidae), thrips (thysanoptera: thripidae), and leafhoppers (homoptera: cicadellidae). | during 1996, 1997, and 1999, studies were conducted in cotton, sugar beets, alfalfa, yardlong bean, and peanut fields to compare insect catches in cc traps equipped with different trap base colors. the studies were conducted in southwestern united states, china, and india. the nine colors, white, rum, red, yellow, lime green, spring green, woodland green (dark green), true blue, and black, varied in spectral reflectance in the visible (400-700 nm) and near-infrared (700-1050 nm) portions of spec ... | 2000 | 10985050 |
| synergy between enzymes from aspergillus involved in the degradation of plant cell wall polysaccharides. | synergy in the degradation of two plant cell wall polysaccharides, water insoluble pentosan from wheat flour (an arabinoxylan) and sugar beet pectin, was studied using several main-chain cleaving and accessory enzymes. synergy was observed between most enzymes tested, although not always to the same extent. degradation of the xylan backbone by endo-xylanase and beta-xylosidase was influenced most strongly by the action of alpha-l-arabinofuranosidase and arabinoxylan arabinofuranohydrolase result ... | 2000 | 10990025 |
| host-correlated morphological variation of myzus persicae (hemiptera: aphididae) populations in greece. | morphological variation in nine characters of 157 clones of myzus persicae (sulzer) was examined by multivariate analysis. the clones were collected from peach, prunus persica, the primary host and the secondary hosts tobacco, nicotiana tabacum, cabbage, brassica oleracea, sugarbeet, beta vulgaris and pepper capsicum annuum. the 156 clones originated from various regions of greece, both in the north, where a large part of the population has an annual bisexual generation on peach, and in more sou ... | 2000 | 10996864 |