| flavones with antibacterial activity against cariogenic bacteria. | methanolic extracts obtained from 13 plants were studied for their antibacterial activity against cariogenic bacteria. among them, the extract from artocarpus heterophyllus showed the most intensive activity. serial chromatographic purifications offered two active compounds which were identified as 6-(3-methyl-1-butenyl)-5,2',4'-trihydroxy-3-isoprenyl-7-methoxy flavone and 5,7,2',4'-tetrahydroxy-6-isoprenylflavone. both isolates completely inhibited the growth of primary cariogenic bacteria at 3 ... | 1996 | 8953432 |
| a novel mode of carbohydrate recognition in jacalin, a moraceae plant lectin with a beta-prism fold. | jacalin, a tetrameric two-chain lectin (66,000 mr) from jackfruit seeds, is highly specific for the tumour associated t-antigenic disaccharide. the crystal structure of jacalin with methyl-alpha-d-galactose reveals that each subunit has a three-fold symmetric beta-prism fold made up of three four-stranded beta-sheets. the lectin exhibits a novel carbohydrate-binding site involving the n terminus of the alpha-chain which is generated through a post-translational modification involving proteolysis ... | 1996 | 8673603 |
| maturation of immunoglobulin-a binding proteins in the seeds of jackfruit. | the accumulation of proteins in the jackfruit seeds was monitored by extracting seeds proteins at various stages of growth. the early developing seed had several protein bands distributed over a molecular weight range of 10-67 kda. but the mature seeds contained fewer bands with two of them being prominent at 12 and 15.4 kda. upon isoelectric focusing, the mature seed proteins were resolved into several bands distributed over a ph range of 4-9. while the mature seed proteins contained powerful h ... | 1995 | 7549923 |
| the isolation and characterisation of jacalin [artocarpus heterophyllus (jackfruit) lectin] based on its charge properties. | jackfruit extracts contain a protein termed jacalin which possesses diverse biological properties. a detailed analysis of its charge properties has been lacking. the present investigation was initiated to study isoelectric properties of jacalin in detail and to isolate a single isoform of jacalin. jacalin was isolated from jackfruit extracts by affinity chromatography on immunoglobulin-a immobilised to sepharose 4b. various techniques such as ion-exchange chromatography, isoelectric focusing (ie ... | 1995 | 7767783 |
| the composition and properties of jacalin, a lectin of diverse applications obtained from the jackfruit (artocarpus heterophyllus) seeds. | | 1994 | 8070846 |
| a novel lectin-based enzyme-linked immunosorbent assay for the measurement of iga1 in serum and secretory iga1 in secretions. | jackfruit lectin (jacalin) specifically binds iga1 and secretory iga1 (siga1). jacalin was successfully purified, and used as the immobilized phase in novel noncompetitive enzyme-linked immunosorbent assays (elisas) for the measurement of iga1 in serum and siga1 in saliva, using jacalin affinity purified material as standards. a non-competitive elisa for the measurement of total siga was also developed using commercially available reagents. | 1993 | 8222271 |
| isolation and characterization of cdna clones encoding jacalin isolectins. | four jacalin cdna clones (pskcja1, pskcja3, pskcja15, and pskcja17) have been obtained from an artocarpus integrifolia (jackfruit) seed cdna library. these clones share over 94% sequence homology, and their deduced polypeptide sequences confirm the existence of multiple jacalin isolectins in jackfruit seeds. the deduced amino acid sequences show that jacalin appears to be initially synthesized as a prepropeptide with the following structure: n-signal (21 residues)-->propeptide (39 residues)-->be ... | 1993 | 7680651 |
| structural and electron-microscopic studies of jacalin from jackfruit (artocarpus integrifolia) show that this lectin is a 65 kda tetramer. | the 133-amino-acid sequences of the alpha-subunit of jacalin (a lectin from artocarpus integrifolia) and of the slightly larger alpha'-subunit were determined. the alpha'- and alpha-subunits, in the approximate ratio of 1:3, were found to be virtually identical in their primary structures, except for one valine for isoleucine substitution at position 113. although both alpha'- and alpha-chains were glycosylated, the extent of glycosylation in the alpha'-chain was much greater than that in the al ... | 1992 | 1520261 |
| preparation and x-ray characterization of four new crystal forms of jacalin, a lectin from artocarpus integrifolia. | four new crystal forms of the anti-t lectin from jackfruit (artocarpus integrifolia) have been prepared and characterized. three of them, two monoclinic (p21, a = 59.4 a, b = 83.3 a, c = 63.5 a, beta = 107.7 degrees; c2, a = 106.1 a, b = 53.9 a, c = 128.0 a, beta = 95.0 a) and one orthorhombic (c222(1), a = 98.1 a, b = 67.3 a, c = 95.1 a) were grown with 2-methylpentan-2,4-diol (mpd) as the precipitant while the fourth, an hexagonal form (p6(1)22, a = b = 129.6 a, c = 157.9 a), was obtained in t ... | 1991 | 1942030 |
| autoantibodies to lactate dehydrogenase in serum identified by use of immobilized protein g and immobilized jacalin, a jackfruit lectin. | in this method for identifying autoantibodies to lactate dehydrogenase (anti-lds) in serum, we used immobilized protein g to bind igg-complexed ld and immobilized jacalin to bind iga-complexed ld, leaving non-complexed ld in solution. the non-complexed ld and total ld were kinetically measured. we report results as ld bound to immobilized protein g and ld bound to immobilized jacalin. using sera demonstrating igg and iga anti-lds by immunoelectrophoresis (iep), respectively, we optimized the met ... | 1989 | 2582615 |
| a basis for estimation of consumption: literature values for selected food volatiles. part iii. | quantitative data on volatile compounds have been reported in 16 food items. no publications reporting quantitative data were found for two of these 16 food products, i.e. avocado and jackfruit. about 550 volatile compounds have been assayed globally in the other 14 food products. mango and raspberry were the products with the greatest number of volatile compounds; the most representative substances were benzaldehyde, ethyl acetate, limonene, and 2-phenylethanol. | 2004 | 2647533 |
| elimination of undesirable immunoglobulin contaminants including aggregated igg from gamma-globulin preparations by jackfruit lectin affinity chromatography. | jackfruit lectin, jacalin, has been characterized to combine with iga of only iga1 subclass but not with iga2 subclass and other immunoglobulins. however, we found in the present study that a lectin from another batch of jackfruit (jacalin-h) showed a different nature in binding reaction. jacalin-h combined with immunoglobulins of every class or subclass except monomer igg. more interestingly, the jacalin-h also combined with aggregated igg. by means of the jacalin-h affinity chromatography, we ... | 1988 | 2454765 |
| jacalin, a jackfruit lectin, precipitates iga1 but not iga2 subclass on gel diffusion reaction. | precipitation reaction in agarose gel between jacalin, a lectin from jackfruit seeds, and purified immunoglobulins of various classes and their components was studied. single precipitation arcs were observed with monomeric and dimeric iga1 subclass as well as secretory iga1, but not with iga2 of both allotypes, igg, igm, igd, ige, free secretory component or with the j chain. thus, jacalin can be used for differentiating iga1 from iga2 subclass by a simple agarose gel diffusion. | 1986 | 3082992 |