| inhibition of listeria monocytogenes by freeze-dried piscicocin cs526 fermentate in food. | the effectiveness of freeze-dried powder, fermented with bacteriocin producing carnobacterium piscicola cs526, was evaluated for the inhibition of listeria monocytogenes in a food model. | 2007 | 17257251 |
| limitations in the use of drosophila melanogaster as a model host for gram-positive bacterial infection. | to examine sensitivities of various drosophila melanogaster strains towards human pathogenic and nonpathogenic gram-positive bacteria. | 2007 | 17257264 |
| short communication: carnobacterium maltaromaticum: the only carnobacterium species in french ripened soft cheeses as revealed by polymerase chain reaction detection. | the pcr technique using cb1-cb2r and species-specific primers was applied to various french soft flowered or washed rind cheeses to identify carnobacterium species. thirty cheeses made from cow's, ewe's, or goat's milk (raw or pasteurized), of which 20 were appellation d'origine contrôlée, were analyzed in the autumn and spring. the results revealed that, irrespective of the season, the carnobacterium genus was initially detected in 5 cheeses and was detected in 5 others after an enrichment peri ... | 2007 | 17297087 |
| diversity of lactic acid bacteria associated with fish and the fish farm environment, established by amplified rrna gene restriction analysis. | lactic acid bacteria have become a major source of concern for aquaculture in recent decades. in addition to true pathogenic species of worldwide significance, such as streptococcus iniae and lactococcus garvieae, several species have been reported to produce occasional fish mortalities in limited geographic areas, and many unidentifiable or ill-defined isolates are regularly isolated from fish or fish products. to clarify the nature and prevalence of different fish-associated bacteria belonging ... | 2007 | 17337536 |
| rapid identification and classification of bacteria by 16s rdna restriction fragment melting curve analyses (rfmca). | the aim of this work was to evaluate restriction fragment melting curve analyses (rfmca) as a novel approach for rapid classification of bacteria during food production. rfmca was evaluated for bacteria isolated from sous vide food products, and raw materials used for sous vide production. we identified four major bacterial groups in the material analysed (cluster i-streptococcus, cluster ii-carnobacterium/bacillus, cluster iii-staphylococcus and cluster iv-actinomycetales). the accuracy of rfmc ... | 2007 | 17367680 |
| isolation and characterization of lactic acid bacteria from lakes. | lactic acid bacteria (lab) were isolated from lake-water samples collected at 7 lakes distributed in yamanashi prefecture, japan. sampling was performed year round. 112 cultures were isolated and divided into classes by phenotype and then into groups by restriction fragment length polymorphism analysis and sequencing of 16s ribosomal dna. phenotypic and biochemical characteristics identified eleven different bacterial groups (a to k), and the results showed that the isolates represented seven ge ... | 2007 | 17440921 |
| identification of new genes associated with intermediate resistance of enterococcus faecalis to divercin v41, a pediocin-like bacteriocin. | it has been suggested that resistance to class iia bacteriocins occurs at either a low or a high level. in listerial strains, low-level resistance (2-4-fold) to class iia bacteriocins is attributed to alterations in membrane lipid composition. in listeria monocytogenes and enterococcus faecalis, high-level resistance (1000-fold) correlates with inactivation of the mptacd operon, which encodes the eii(man)(t) mannose permease of the phosphotransferase system (pts). previous studies reported that ... | 2007 | 17464076 |
| action of divergicin m35, a class iia bacteriocin, on liposomes and listeria. | the mode of action of divergicin m35, a class iia bacteriocin, was studied against listeria monocytogenes with sensitive (divs) and resistant (divm) phenotypes, as well as on synthetic phospholipid liposomes. | 2007 | 17578415 |
| the bacterial flora of vacuum-packed cold-smoked salmon stored at 7 degrees c, identified by direct 16s rrna gene analysis and pure culture technique. | the indigenous flora of freshly chilled cold-smoked salmon just after the vacuum packaging, and the spoilage flora after storage, in vacuum package at 7 degrees c for 19 days, were to be investigated with two different sampling strategies. | 2007 | 17584457 |
| molecular dynamics simulation study of interaction between a class iia bacteriocin and its immunity protein. | molecular dynamics (md) simulations of carnobacteriocin b2 (cbnb2), a structurally well-characterized class iia bacteriocin, and its immunity protein (imb2) in lipid bilayer environment have been conducted to explore the interaction between them. six 30-ns simulations were conducted in dppc or popg bilayer systems. in these simulations, imb2 was placed in the aqueous layer with different orientations facing cbnb2 to sample all the faces of imb2. the md results indicate that (i) while cbnb2 remai ... | 2007 | 17586105 |
| development of innovative pediocin pa-1 by dna shuffling among class iia bacteriocins. | pediocin pa-1 is a member of the class iia bacteriocins, which show antimicrobial effects against lactic acid bacteria. to develop an improved version of pediocin pa-1, reciprocal chimeras between pediocin pa-1 and enterocin a, another class iia bacteriocin, were constructed. chimera ep, which consisted of the c-terminal half of pediocin pa-1 fused to the n-terminal half of enterocin a, showed increased activity against a strain of leuconostoc lactis isolated from a sour-spoiled dairy product. t ... | 2007 | 17601819 |
| class i/class iia bacteriocin cross-resistance phenomenon in listeria monocytogenes. | variants resistant to nisin a (va), nisin z (vz), pediocin pa-1 (vp), divergicin m35 (vd) and to bacteriocin-like compounds produced by bifidobacterium thermophilum subsp. infantis rbl67 (vb) were developed from listeria monocytogenes lsd530. lactic acid production, specific growth rate, potassium ion efflux, susceptibility to 13 antibiotics, cell-envelope fatty acid composition and bacteriocin cross-resistance were evaluated. lactic acid production decreased to 75% or less of that by strain lsd ... | 2007 | 17613369 |
| carnobacterium: positive and negative effects in the environment and in foods. | the genus carnobacterium contains nine species, but only c. divergens and c. maltaromaticum are frequently isolated from natural environments and foods. they are tolerant to freezing/thawing and high pressure and able to grow at low temperatures, anaerobically and with increased co(2) concentrations. they metabolize arginine and various carbohydrates, including chitin, and this may improve their survival in the environment. carnobacterium divergens and c. maltaromaticum have been extensively stu ... | 2007 | 17696886 |
| antimicrobial activity of divercin rv41 produced and secreted by lactococcus lactis. | divercin v41 is a class iia bacteriocin produced by carnobacterium divergens v41 with a strong anti-listeria activity. we have previously produced a recombinant form of divercin v41 (dvnrv41) in escherichia coli strain origami, by cloning a synthetic gene that codes for a mature divercin rv41 peptide. in this work we describe the inducible expression and secretion of dvnrv41 in the food-grade lactic acid bacterium, lactococcus lactis. the production of dvnrv41 by recombinant l. lactis was confir ... | 2007 | 17827978 |
| spoilage of value-added, high-oxygen modified-atmosphere packaged raw beef steaks by leuconostoc gasicomitatum and leuconostoc gelidum. | moisture-enhancing and marinating of meats are commonly used by the meat industry to add value to raw, retail products. recently in finland, certain value-added beef steak products have proven to be unusually susceptible to microbial spoilage leading to untoward quality deteriorations during producer-defined shelf-life. this study was conducted to evaluate the role of lactic acid bacteria (lab) in the premature spoilage of value-added beef packaged under high-oxygen modified atmospheres. spoilag ... | 2007 | 17913272 |
| structure function relationship of inducer peptide pheromones involved in bacteriocin production in carnobacterium maltaromaticum and enterococcus faecium. | the production of several bacteriocins in lactic acid bacteria is regulated by inducer peptide pheromones that specifically interact with their cognate bacterial receptor. these peptide pheromones are between 19 and 27 aa long and contain a conserved (v/i)-x-x-x-f sequence followed by positively charged residues in the c-terminal domain. cbax and entf are peptide pheromones that share similarity and are involved in the production of carnobacteriocin a in carnobacterium maltaromaticum lv17a and e ... | 2007 | 17975073 |
| quality changes during refrigerated storage of ma-packaged pre-rigor fillets of farmed atlantic cod (gadus morhua l.) using traditional map, co2 emitter, and vacuum. | quality changes during 3 wk of refrigerated storage (1.3 degrees c) were studied on pre-rigor filleted farmed atlantic cod packed in modified atmosphere (map, 60% co2 and 40% o2) or vacuum. the packages of map contained either a co2 emitter and low gas volume to product volume (g/p ratio) of 1.3, or a 3.9 g/p ratio and no emitter. the co2 level remained stable or increased in the packages with co2 emitter, whereas the co2 level in the packages with no co2 emitter decreased to 40% after 4 d of re ... | 2007 | 18034737 |
| evaluation of the spoilage lactic acid bacteria in modified-atmosphere-packaged artisan-type cooked ham using culture-dependent and culture-independent approaches. | to investigate microbial diversity and population dynamics of spoilage-sensitive modified-atmosphere-packaged (map) artisan-type cooked ham in relation to storage temperature. | 2008 | 18070037 |
| genetic and biochemical characterization of cad-1, a chromosomally encoded new class a penicillinase from carnobacterium divergens. | carnobacterium divergens clinical isolates bm4489 and bm4490 were resistant to penicillins but remained susceptible to combinations of amoxicillin-clavulanic acid and piperacillin-tazobactam. cloning and sequencing of the responsible determinant from bm4489 revealed a coding sequence of 912 bp encoding a class a beta-lactamase named cad-1. the bla(cad-1) gene was assigned to a chromosomal location in the two strains that had distinct pulsed-field gel electrophoresis patterns. cad-1 shared 53% an ... | 2008 | 18070972 |
| effect of low temperature on stability of theta-type plasmids in carnobacterium maltaromaticum. | the heterologous production of useful peptides such as bacteriocins by lactic acid bacteria (lab) has been studied for use in the biopreservation of foods. recombinant plasmids can suffer drawbacks such as segregational instability affecting the production of these peptides in certain environments such as absence of selective pressure or low temperature. the link between growth temperature characteristics of parental strains and stability of theta-type plasmids at a low temperature was investiga ... | 2008 | 18179588 |
| analysis of the intestinal microflora in hepialus gonggaensis larvae using 16s rrna sequences. | gut microbial diversity provides insight into the basic function of a gut microbial ecosystem. in this study, restriction fragment length polymorphism 16s rrna sequences was used to detect the intestinal microbial diversity of hepialus gonggaensis larvae. the total dna of microorganisms was extracted from the intestinal contents and 16s rrna was amplified. a nearly full-length of 16s rrna sequence library was constructed. the fingerprints of the microorganisms were analyzed by isolating plasmid ... | 2008 | 18183461 |
| binding of nontarget microorganisms from food washes to anti-salmonella and anti-e. coli o157 immunomagnetic beads: most probable composition of background eubacteria. | we present herein the composition of bacterial communities occurring in ground chicken and the changes which arise in these populations based upon nonselective partitioning by commercially-available dynal anti-salmonella and anti-e. coli o157 immunomagnetic beads (imb). our enumeration and colony selection protocol was based upon a 6 × 6 drop plate method (n = 18 for each 25-g sub-sampling) using a dilution which resulted in ca. 4-8 colonies per drop. an average of 82 ± 13 colonies were selected ... | 2008 | 18311564 |
| optimization of the production and purification processes of carnobacteriocins cbn bm1 and cbn b2 from carnobacterium maltaromaticum cp5 by heterologous expression in escherichia coli. | an optimization of the production and purification processes of carnobacteriocins cbn bm1 and cbn b2 from carnobacterium maltaromaticum cp5, by heterologous expression in escherichia coli is described. the genes encoding mature bacteriocin were cloned into an e. coli expression system and expressed as a fusion protein with a thermostable thioredoxin. recombinant e. coli were cultivated following a fed-batch fermentation process with ph, temperature and oxygenation regulation. the overexpression ... | 2008 | 18316133 |
| m35 oxidation induces abeta40-like structural and dynamical changes in abeta42. | oxidizing a single m35 residue of abeta leads to delayed aggregation and reduced toxicity. to understand the molecular mechanism of this effect, we examined the structural and dynamical consequences of m35 oxidation. we found the mobility of the c-terminal residues of abeta42 is greatly enhanced upon m35 oxidation. in contrast, methyl groups in the central hydrophobic cluster become less flexible. taken together, we conclude that abeta42ox undergoes abeta40-like structural and dynamical changes, ... | 2008 | 18376837 |
| effect of antimicrobial peptides divergicin m35 and nisin a on listeria monocytogenes lsd530 potassium channels. | the aim of this work was to study the effect of antimicrobial peptides: divergicin m35 and nisin a on listeria monocytogenes lsd 530 potassium (k+) channels: atp-sensitive (k atp), calcium-activated (bk ca), and depolarization-activated (kv) types. increase on k+ efflux and inhibition of cellular growth were observed after adding k+ channel activators pinacidil, ns1619, and cromakalim to divergicin m35. increase in k+ efflux from log-phase cells was about 18 +/- 1.1, 11 +/- 0.63, and nmol mg(-1) ... | 2008 | 18379845 |
| alpha-chitinase activity among lactic acid bacteria. | chitin is a polysaccharide widely distributed in nature. among 115 strains from 29 species of lactic acid bacteria only strains belonging to carnobacterium divergens and carnobacterium maltaromaticum hydrolyzed alpha-chitin. this activity was not affected by temperature (10 degrees c versus 30 degrees c) and in most cases not subject to glucose catabolite repression. | 2008 | 18424038 |
| microbial changes and growth of listeria monocytogenes during chilled storage of brined shrimp (pandalus borealis). | thirteen storage trials and ten challenge tests were carried out to examine microbial changes, spoilage and the potential growth of listeria monocytogenes in brined shrimp (pandalus borealis). shrimp in brine as well as brined and drained shrimp in modified atmosphere packaging (map) were produced and studied. different recipes were used to study the effect of preserving parameters (organic acids, ph and nacl) on growth of microorganisms and shelf life at 7-8 degrees c or 12 degrees c. particula ... | 2008 | 18456355 |
| isolation and characterization of carnocyclin a, a novel circular bacteriocin produced by carnobacterium maltaromaticum ual307. | carnobacterium maltaromaticum ual307, isolated from fresh pork, exhibits potent activity against a number of gram-positive organisms, including numerous listeria species. three bacteriocins were isolated from culture supernatant, and using matrix-assisted laser desorption ionization-time of flight mass spectrometry and edman sequencing, two of these bacteriocins were identified as piscicolin 126 and carnobacteriocin bm1, both of which have previously been described. the remaining bacteriocin, wi ... | 2008 | 18552180 |
| study of the bacterial ecosystem in tropical cooked and peeled shrimps using a polyphasic approach. | the characterization of the microbial ecosystem of cooked tropical shrimps was carried out using a polyphasic approach. first, culture-dependent methods were used for bacterial enumeration and the phenotypic and molecular identification of bacterial isolates. then, culture-independent methods, including pcr-ttge (v3 region of the 16s rrna gene), provided a fingerprinting of bacterial dna directly extracted from shrimps. two batches of cooked and peeled tropical shrimps were stored at 5 and 15 de ... | 2009 | 18573557 |
| [analysis of the bacterial diversity in intestines of hepialus gonggaensis larvae]. | we investigated the intestinal microbial diversity in the larval gut of hepialus gonggaensis, an economically important insect. | 2008 | 18652293 |
| isolation of a carnobacterium maltaromaticum- like bacterium from systemically infected lake whitefish (coregonus clupeaformis). | herein we report on the first isolation of a carnobacterium maltaromaticum-like bacterium from kidneys and swim bladders of lake whitefish (coregonus clupeaformis) caught from lakes michigan and huron, michigan. isolates were gram-positive, nonmotile, facultatively anaerobic, asporogenous rods that did not produce catalase, cytochrome oxidase, or h2s, and did not grow on acetate agar. except for carbohydrate fermentation, many phenotypic characteristics of lake whitefish isolates coincided with ... | 2008 | 18793198 |
| short communication: impact of ph and temperature on the acidifying activity of carnobacterium maltaromaticum. | the acidifying activity of carnobacterium maltaromaticum lma28, a strain isolated from french soft cheese, was studied in trypticase soy broth with yeast extract (tsb-ye) medium and in milk. in tsb-ye supplemented with lactose, glucose, or galactose, lactose and glucose were metabolized with a maximum growth rate of 0.32 h(-1) and galactose was not metabolized. during hydrolysis of lactose, the galactose moiety was not excreted. the major product was l(+) lactic acid, with no significant differe ... | 2008 | 18832202 |
| screening and characterisation of potentially pathogenic bacteria associated with atlantic cod gadus morhua larvae: bath challenge trials using a multidish system. | in intensive aquaculture systems, high concentrations of nutrients and high densities of fish larvae provide favorable conditions for opportunistic pathogenic bacteria to flourish. we screened potentially pathogenic bacterial strains isolated from moribund atlantic cod gadus morhua larvae, pollack pollachius pollachius, coalfish pollachius virens, atlantic halibut hippoglossus hippoglossus, rotifers, algae and water samples from different hatcheries. three identical challenge experiments tested ... | 2008 | 18998585 |
| divercin v41 from gene characterization to food applications: 1998-2008, a decade of solved and unsolved questions. | the emergence of an increasing number of antibiotic resistant human clinical bacteria has been a great cause of concern for the last decades. as an example, staphylococcus aureus isolates in the hospital environment are becoming more and more resistant to antibiotics including vancomycin which is considered as a last line of defence in treatment of staphylococcus aureus-resistant methicillin. on the other hand, food safety is threatened by development of pathogenic bacteria including listeria mo ... | 2009 | 19018960 |
| large scale purification protocol for carnocin kz 213 from carnobacterium piscicola. | large scale purification of a class iia bacteriocin has been developed to recover pure carnocin kz 213 produced by carnobacterium piscicola 213. most previous protocols reported in the literature for the purification of small peptides have used reversed phase chromatography but scale-up is difficult. the first step of this new protocol is hydrophobic interaction chromatography, the second and third steps are cation exchange chromatography. the protocol leads to a complete recovery of carnocin kz ... | 2009 | 19066731 |
| identification of a new molecular target of class iia bacteriocins in listeria monocytogenes egde. | it was shown recently (calvez et al. 2007) that glpq and pde genes are involved in intermediate resistance of enterococcus faecalis jh2-2 to dvnv41, a class iia bacteriocin produced by carnobacterium divergens v41. the listerial orthologs of lpq and pde genes might be lmo0052 and lmo1292 genes, respectively. here, the role of lmo0052 and lmo1292 genes in resistance of listeria monocytogenes egde to dvnv41 and mesy105 was investigated. l. monocytogenes egde was inactivated in lmo0052 and/or lmo12 ... | 2008 | 19085076 |
| carnobacterium species: effect of metabolic activity and interaction with brochothrix thermosphacta on sensory characteristics of modified atmosphere packed shrimp. | the importance of carnobacteria as spoilage microorganisms or potential protective cultures in food is not resolved, and little is known about their metabolism during growth in specific products. this study used chromatographic techniques including gc-ms and hplc to evaluate the spoilage metabolism of carnobacterium divergens, carnobacterium maltaromaticum, and carnobacterium mobile. metabolic activity was studied in cooked and peeled modified atmosphere packed (map) shrimp at 5 degrees c as car ... | 2006 | 19127732 |
| insights into structure-activity relationships in the c-terminal region of divercin v41, a class iia bacteriocin with high-level antilisterial activity. | divercin v41 (dvnv41) is a class iia bacteriocin with potent antilisterial activity isolated from carnobacterium divergens v41. previously, we expressed from a synthetic gene, in escherichia coli origami, a recombinant dvnv41 designated dvnrv41, which possesses four additional amino acids (amdp) in the n-terminal region that result from enzymatic cleavage and retains the initial dvnv41 activity. to unravel the relationship between the structure of dvnrv41 and its particularly elevated activity, ... | 2009 | 19181835 |
| selected nondigestible carbohydrates and prebiotics support the growth of probiotic fish bacteria mono-cultures in vitro. | to search for nondigestible but fermentable (ndf) carbohydrates and prebiotics with a potency to promote the growth of selected bacteria in vitro. | 2009 | 19191975 |
| lactic acid bacteria associated with a heat-processed pork product and sources of variation affecting chemical indices of spoilage and sensory characteristics. | to evaluate the potential for developing a quality index for a danish modified atmosphere packaged (map) heat-processed and naturally contaminated pork meat product stored at 5 degrees c. | 2009 | 19200320 |
| mesophilic and psychrotrophic bacteria from meat and their spoilage potential in vitro and in beef. | mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (voc) by gas chromatography-mass spectrometry (gc/ms). fifty mesophilic and twenty-nine psychrotrophic isolates were analyzed by random amplified polymorphic dna-pcr, and representative strains were identified by 16s rrna gene sequencing. carnobacterium maltaromaticum ... | 2009 | 19201980 |
| comparison of culture-dependent and independent techniques for characterisation of the microflora of peroxyacetic acid treated, vacuum-packaged beef. | the diversity of microflora associated with peroxyacetic acid (poaa) treated and untreated beef was investigated by 16s rdna gene cloning, dgge analysis and conventional bacterial cultivation. following vacuum packaging, poaa treated and untreated meat samples were stored for up to 18 weeks at -1.5 degrees c. each culture independent method showed carnobacterium spp. to predominate on both poaa treated and untreated meat. however, 16s rdna gene analysis also detected the presence of psychrotoler ... | 2009 | 19269570 |
| interactions between two carnobacteriocins cbn bm1 and cbn b2 from carnobacterium maltaromaticum cp5 on target bacteria and caco-2 cells. | two purified class iia carnobacteriocins cbn bm1 and cbn b2, from carnobacterium maltaromaticum cp5, were evaluated for antimicrobial activity against pathogenic, spoilage and lactic acid bacteria. then, the presence of a synergistic mode of action of these two carnobacteriocins on listeria sp., enterococcus sp. and carnobacterium sp. was investigated. a synergistic mode of action between cbn bm1 and cbn b2 on sensitive target bacteria was demonstrated using the fic index method. combinations of ... | 2009 | 19271288 |
| development of a new method for the detection of lactic acid bacteria capable of protecting ham against enterobacteriaceae. | challenge trials seem to be the best assessment approach to evaluate the potency of food protective cultures. however, this method is time consuming and often difficult to implement. here, we describe the development of the 'sequential culturing method', a new method for the screening of strains as protective cultures. | 2009 | 19291212 |
| characterization of the bacterial spoilage flora in marinated pork products. | to investigate the microbiota in marinated, vacuum-packed pork and to characterize isolated bacteria with regard to their spoilage potential. | 2009 | 19298510 |
| fate of food-associated bacteria in pork as affected by marinade, temperature, and ultrasound. | the aim of this study was to test the effect of ultrasound, red wine, and yogurt marination on brochotrix thermosphacta, carnobacterium maltaromaticum, listeria monocytogenes, and campylobacter jejuni on pork meat. two different marination procedures of the pork medallions were tested: (i) submersion of meat medallions in red wine during the entire experiment and (ii) vacuum packaging of meat medallions after different forms of marination. in the submersion procedure, the meat was either submerg ... | 2009 | 19343943 |
| psychrotrophic lactic acid bacteria used to improve the safety and quality of vacuum-packaged cooked and peeled tropical shrimp and cold-smoked salmon. | previously isolated lactic acid bacteria (lab) from seafood products have been investigated for their capacity to increase the sensory shelf life of vacuum-packaged shrimp and cold-smoked salmon and to inhibit the growth of three pathogenic bacteria. two different manufactured batches of cooked, peeled, and vacuum-packaged shrimp were inoculated with seven lab strains separately at an initial level of 5 log cfu g-t, and the spoilage was estimated by sensory analysis after 7 and 28 days of storag ... | 2009 | 19350982 |
| the circular bacteriocin, carnocyclin a, forms anion-selective channels in lipid bilayers. | bacterial resistance to conventional antibiotics is a major challenge in controlling infectious diseases and has necessitated the development of novel approaches in antimicrobial therapy. one such approach is the use of antimicrobial peptides, such as the bacterially produced bacteriocins. carnocyclin a (ccla) is a 60-amino acid circular bacteriocin produced by carnobacterium maltaromaticum ual307 that exhibits potent activity against many gram-positive bacteria. lipid bilayer and single channel ... | 2009 | 19463781 |
| microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes. | in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ... | 2009 | 19477544 |
| interaction of an antimicrobial peptide with a model lipid bilayer using molecular dynamics simulation. | we present results of molecular dynamics simulations of the interaction of a positively charged antimicrobial peptide, carnobacteriocin b2, with a mixed (anionic-zwitterionic) lipid bilayer carrying a net negative charge. when the peptide is initially immersed in an aqueous medium, it approaches the bilayer surface because of electrostatic attraction. insertion of the single peptide in the bilayer, however, is not spontaneous. simulations are also conducted by employing initial configurations wh ... | 2009 | 19505152 |
| a novel packaging method with a dissolving co(2) headspace combined with organic acids prolongs the shelf life of fresh salmon. | the aim of this study was to evaluate a novel packaging method for fresh fish and to determine its effect on the bacterial growth in fresh salmon. fresh salmon was packed with a small amount of 100% co(2) (gas/product ratio 0.2/1.0 v/v) and a brine solution containing various combinations of citric acid (3% w/w, ph 5), acetic acid (1% w/w, ph 5) and cinnamaldehyde (200 microg/ml). total bacterial counts, counts of sulphur reducing bacteria, lactic acid bacteria and enterobacteriaceae as well as ... | 2009 | 19523706 |
| selection and evaluation of seafood-borne psychrotrophic lactic acid bacteria as inhibitors of pathogenic and spoilage bacteria. | in this study, inhibitory psychrotrophic lactic acid bacteria were isolated and investigated for future use in biopreservation of seafood products. screening of 5575 colonies isolated from various seafood products resulted in the selection of 132 colonies presenting inhibitory properties. among them, 52 isolates had characteristics of lab and showed growth at 15 degrees c but not at 30 degrees c. the inhibition spectrum of these 52 isolates against 14 target strains (gram-positive and -negative) ... | 2009 | 19527840 |
| characterization of probiotic carnobacteria isolated from rainbow trout (oncorhynchus mykiss) intestine. | to characterize two probiotic carnobacterial isolates, carnobacterium maltaromaticum (b26) and c. divergens (b33), derived from rainbow trout (oncorhynchus mykiss) intestine. | 2008 | 19552776 |
| bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of german red smear soft cheese. | the phylogenetic position and physiological characters of six hitherto-unknown lactic acid bacterial isolates, which form part of the surface microbiota of german red smear soft cheese, are reported. the coccoid cells are aerotolerant, gram-positive, catalase-negative and non-motile. the cell-wall peptidoglycan contains alanine, glutamic acid, lysine and aspartic acid and is of the a4alpha type (l-lys-d-asp). the sequences of the 16s rrna, groel and rpob genes of the six isolates are identical a ... | 2009 | 19622668 |
| carnobacterium jeotgali sp. nov., isolated from a korean traditional fermented food. | a gram-positive, facultatively anaerobic bacterium, designated strain ms3(t), was isolated from a traditional korean fermented food made with freshwater shrimp. strain ms3(t) was able to grow at 4-37 degrees c, at ph 5.5-9.0 and in the presence of 0-5 % (w/v) nacl. optimal growth occurred at 30 degrees c, at ph 8.5 and in the presence of 2 % nacl. the strain was catalase- and oxidase-negative. it was able to metabolize various carbohydrates as energy sources. 16s rrna gene sequence analysis show ... | 2009 | 19643884 |
| survival of listeria monocytogenes, listeria innocua, and lactic acid bacteria in chill brines. | listeria monocytogenes is the pathogen of concern in ready-to-eat (rte) meat products. salt brines are used to chill processed meats. l. monocytogenes and lactic acid bacteria (lab) can grow under saline conditions, and may compete with each other for nutrients. the objective of this study was to determine the effect of lactic acid bacteria (enterococcus faecalis, carnobacterium gallinarum, and lactobacillus plantarum) on the survival of l. monocytogenes and listeria innocua in brines stored und ... | 2009 | 19646051 |
| effects of bacterial treatment at early stages of atlantic cod (gadus morhua l.) on larval survival and development. | to assess the effects of bacterial treatment at the earliest stages of cod rearing on the microbial load, larval development and performance, testing three bacterial strains (carnobacterium divergens v41, arthrobacter sp. and enterococcus sp.) in vivo that were previously shown to have inhibitory potential towards fish pathogens in vitro. | 2010 | 19664064 |
| the three-dimensional structure of carnocyclin a reveals that many circular bacteriocins share a common structural motif. | carnocyclin a (ccla) is a potent antimicrobial peptide from carnobacterium maltaromaticum ual307 that displays a broad spectrum of activity against numerous gram-positive organisms. an amide bond links the n and c termini of this bacteriocin, imparting stability and structural integrity to this 60-amino acid peptide. ccla interacts with lipid bilayers in a voltage-dependent manner and forms anion selective pores. several other circular bacteriocins have been reported, yet only one (enterocin as- ... | 2009 | 19692336 |
| culturable prokaryotic diversity of deep, gas hydrate sediments: first use of a continuous high-pressure, anaerobic, enrichment and isolation system for subseafloor sediments (deepisobug). | deep subseafloor sediments may contain depressurization-sensitive, anaerobic, piezophilic prokaryotes. to test this we developed the deepisobug system, which when coupled with the hyacinth pressure-retaining drilling and core storage system and the press core cutting and processing system, enables deep sediments to be handled without depressurization (up to 25 mpa) and anaerobic prokaryotic enrichments and isolation to be conducted up to 100 mpa. here, we describe the system and its first use wi ... | 2009 | 19694787 |
| comparison of different application strategies of divergicin m35 for inactivation of listeria monocytogenes in cold-smoked wild salmon. | cold-smoked salmon treated with divergicin m35-producing carnobacterium divergens m35, c. divergens atcc 35677 (a non-producer of bacteriocin), purified divergicin m35 or supernatants of c. divergens m35 culture in snow crab hepatopancreas (sch) medium or mrs broth was challenged with listeria monocytogenes (up to 10(3) cfu/g). samples were stored at 4 degrees c for up to four weeks. l. monocytogenes, total bacterial and lactic acid bacterial counts were determined along with changes in total vo ... | 2009 | 19835762 |
| effects of temperature and ph on the growth of bacteria isolated from blown packs of vacuum-packaged beef. | bacteria recovered from the microflora of blown packs of vacuum-packaged beef were identified as leuconostoc mesenteroides, lactococcus lactis, carnobacterium maltaromaticum, and clostridium estertheticum, with l. mesenteroides predominant. isolates of these lactic acid bacteria all grew in peptone yeast extract glucose starch broth (pygsb) at temperatures between -2 and 30 degrees c but generally grew more slowly and over a more restricted temperature range in meat juice medium (mjm). a c. este ... | 2009 | 19903404 |
| diversity of lactic acid bacteria from modified atmosphere packaged sliced cooked meat products at sell-by date assessed by pcr-denaturing gradient gel electrophoresis. | the predominant lactic acid bacteria (lab) microbiota associated with three types of modified atmosphere packaged (map) sliced cooked meat products (i.e. ham, turkey and chicken) was analyzed at sell-by date using a combination of culturing and molecular population fingerprinting. likewise routine analyses during industrial map production, meat samples were plated on the general heterotrophic plate count agar (pca) and on the lab-specific de man, rogosa, sharpe (mrs) agar under different tempera ... | 2010 | 19913685 |
| technology-induced selection towards the spoilage microbiota of artisan-type cooked ham packed under modified atmosphere. | the microbiota associated with a highly-perishable belgian artisan-type cooked ham was analyzed through plating and (gtg)(5)-fingerprinting of isolates throughout its processing chain. the raw tumbled meat was characterized by the presence of a versatile microbiota around 4.8 log(cfu g(-1)), consisting of lactic acid bacteria, staphylococci, brochothrix thermosphacta, gram-negative bacteria, and yeasts. pasteurisation of the ham logs reduced bacterial counts below 2 log(cfu g(-1)) and subsequent ... | 2010 | 19913696 |
| isolation of carnobacterium sp. from a human blood culture. | carnobacterium species have been isolated from the environment and are not regarded as human pathogens, although they are known to cause disease in fish. only two reports describing isolation of carnobacterium species from human pus were found in the literature. we report what we believe to be the first isolation of carnobacterium sp. from a human blood culture. | 2010 | 20075110 |
| surface microbiota analysis of taleggio, gorgonzola, casera, scimudin and formaggio di fossa italian cheeses. | the composition of the bacterial consortia of the smear italian cheeses and their role on quality and safety is still poorly understood. the objective of this study was to identify and characterize the bacterial communities present on the surface of five traditional italian cheeses, casera valtellina, scimudin, formaggio di fossa, gorgonzola and taleggio. dgge analysis performed using total dna obtained from cheese surfaces enabled us to identify the dominant bacterial populations. bands showing ... | 2010 | 20167385 |
| probiotics and immunity: a fish perspective. | probiotics are usually live microorganisms which when administered in adequate amounts confer a health benefits on host. nowadays, probiotics are also becoming an integral part of the aquaculture practices to obtain high production. the common probiotics that are used for aquaculture practices include lactobacillus, lactococcus, leuconostoc, enterococcus, carnobacterium, shewanella, bacillus, aeromonas, vibrio, enterobacter, pseudomonas, clostridium, and saccharomyces species. the involvement of ... | 2010 | 20219683 |
| [changes of intestinal microflora in hepialus gonggaensis larvae after feeding with carnobacterium sp. hg4-03 as a probiotic strain]. | we investigated the influence of carnobacterium sp. hg4-03 on intestinal microflora of the 4th instar hepialus gonggaensis larvae, an economically important insect. | 2010 | 20387469 |
| evaluation of microbial dynamics during the ripening of a traditional taiwanese naturally fermented ham. | isolation and identification of the autochthonous starter from a naturally fermented meat allows control of the fermentation process and promises microbiological safety for this specialty. thus the purpose of this study was to identify the lactic acid bacteria and coagulase-negative cocci present in a traditional taiwanese naturally fermented ham (tnfh) and to study the microbial dynamics at different ripening stages; the approach was a combination of conventional microbiological cultivation, po ... | 2010 | 20417394 |
| evaluation of natural antimicrobials on typical meat spoilage bacteria in vitro and in vacuum-packed pork meat. | the aim of this study was to investigate the inhibitory effect of natural antimicrobials on the growth of typical spoilage bacteria from marinated pork. minimum inhibitory concentrations (mic) of thymol, cinnamaldehyde, allyl isothiocyanate, citric acid, ascorbic acid, a rosemary extract, and a grapefruit seed extract against lactobacillus algidus, leuconostoc mesenteroides, leuconostoc carnosum, carnobacterium maltaromaticum, carnobacterium divergens, brochothrix thermosphacta, and serratia pro ... | 2010 | 20492248 |
| carnobacterium maltaromaticum: identification, isolation tools, ecology and technological aspects in dairy products. | carnobacterium species constitute a genus of lactic acid bacteria (lab) present in different ecological niches. the aim of this article is to summarize the knowledge about carnobacterium maltaromaticum species at different microbiological levels such as taxonomy, isolation and identification, ecology, technological aspects and safety in dairy products. works published during the last decade concerning c. maltaromaticum have shown that this non-starter lab (nslab) could present major interests in ... | 2010 | 20510773 |
| defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions. | a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ... | 2010 | 20537393 |
| bacterial species associated with traditional starter cultures used for fermented bamboo shoot production in manipur state of india. | soidon is a non-salted acidic fermented food prepared from the succulent bamboo shoot tip of schizostachyum capitatum munro by using a traditional liquid starter called "soidon mahi" in manipur state of india. in this study, 163 bacterial isolates associated with this starter samples were identified and their population distribution was investigated by amplified ribosomal dna restriction analysis (ardra), 16s rdna sequencing and randomly amplified polymorphic dna (rapd) analysis. this acidic sta ... | 2010 | 20696489 |
| a new α-galactosidase from symbiotic flavobacterium sp. tn17 reveals four residues essential for α-galactosidase activity of gastrointestinal bacteria. | the α-galactosidase gene, gala17, was cloned from flavobacterium sp. tn17, a symbiotic bacterium isolated from the gut of batocera horsfieldi larvae. the 2,205-bp full-length gene encodes a 734-residue polypeptide (gala17) containing a putative 28-residue signal peptide and a catalytic domain belonging to glycosyl hydrolase family 36 (gh 36). the deduced amino acid sequence of gala17 was most similar to a putative α-galactosidase from pedobacter sp. bal39 (edm38577; 66.6% identity) and a charact ... | 2010 | 20714719 |
| biphasic toxicodynamic features of some antimicrobial agents on microbial growth: a dynamic mathematical model and its implications on hormesis. | in the present work, we describe a group of anomalous dose-response (dr) profiles and develop a dynamic model that is able to explain them. responses were obtained from conventional assays of three antimicrobial agents (nisin, pediocin and phenol) against two microorganisms (carnobacterium piscicola and leuconostoc mesenteroides). | 2010 | 20723220 |
| changes in the bacterial communities of vacuum-packaged pork during chilled storage analyzed by pcr-dgge. | in this study, pcr-denaturing gradient gel electrophoresis (dgge) was used to investigate the bacterial communities of vacuum-packaged pork during chilled storage. eight kinds of lactic acid bacteria (lab) were identified from the strains isolated from mrs plates by pcr-dgge of the v3 region, and lactobacillus sakei was the representative isolate at the end of the monitoring. by means of the direct meat analysis of pcr-dgge, lab increased gradually and carnobacterium sp./car. divergens, lactobac ... | 2010 | 20800372 |
| interactions between bacterial isolates from modified-atmosphere-packaged artisan-type cooked ham in view of the development of a bioprotective culture. | growth and metabolite production of three dominant bacterial isolates (carnobacterium divergens 3m14, leuconostoc carnosum 3m42, and brochothrix thermosphacta rms6) from modified-atmosphere-packaged (map), artisan-type cooked ham were assessed for their interactions in view of the development of a bioprotective culture. during monoculture experiments in cooked ham simulation medium, leuc. carnosum 3m42 converted the available glucose into lactic acid and ethanol, whereas the two other strains pr ... | 2010 | 20832689 |
| spoilage-related microbiota associated with chilled beef stored in air or vacuum pack. | in order to study the spoilage-related microbiota of beef at species level, a combination of culture-independent and culture-dependent methods was used to analyse nine different beef samples stored at 4°c in air or in vacuum pack. plate counts on selective agars after 0, 7 and 20 days of storage showed that vacuum packaging reduced the viable counts of brochothrix thermosphacta, pseudomonas spp. and enterobacteriaceae, whereas the growth of lactic acid bacteria (lab) was unaffected. storage in v ... | 2010 | 21056779 |
| tetracycline resistance associated with commensal bacteria from representative ready-to-consume deli and restaurant foods. | proper knowledge of antibiotic resistance (ar) dissemination is essential for effective mitigation. this study examined the profiles of tetracycline-resistant (tetr) commensal bacteria from representative ready-to-consume food samples from salad bars at local grocery stores and restaurants. out of 900 tetr isolates examined, 158 (17.6%) carried one or more of tetm, tetl, tets, and tetk genes by conventional pcr, 28 harbored more than one tetr determinants. the most prevalent genotype was tetm, w ... | 2010 | 21067672 |
| characterization of psychrotrophic bacterial communities in modified atmosphere-packed meat with terminal restriction fragment length polymorphism. | characterization of psychrotrophic lactic acid bacteria (lab) and brochothrix thermosphacta communities is needed to understand the microbial ecology of spoilage of modified atmosphere-packed (map) meats. to overcome the limitations of the currently used methods for the characterization of psychrotrophic bacterial communities in meat, we developed a culture-independent, 16s rrna gene-targeted terminal restriction fragment length polymorphism (t-rflp) method. an identification library consisting ... | 2010 | 21093087 |
| effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan. | chitosan is a biopolymer with antimicrobial activity and film-forming properties. in this study, the effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan was evaluated. a chitosan was selected from eight types (four non-commercial and four commercial) based on its antimicrobial activity against salmonella enterica serovar enteritidis (s. enteritidis). for this purpose, a contact plate method was developed and chitosans were applied at a concen ... | 2010 | 21146239 |
| the activity of bacteriocins from carnobacterium maltaromaticum ual307 against gram-negative bacteria in combination with edta treatment. | bacteriocins from gram-positive bacteria are potent antimicrobial peptides that inhibit pathogenic and food-spoilage bacteria. they are usually ineffective against gram-negative bacteria because they cannot penetrate the outer membrane (om). disruption of the om of some gram-negative bacteria was reported to sensitize them to certain bacteriocins. this study evaluates the activity of three purified bacteriocins [carnocyclin a (ccla), carnobacteriocin bm1 (cbnbm1) and piscicolin 126 (pisa)] produ ... | 2011 | 21255070 |
| production of biogenic amines by lactic acid bacteria and enterobacteria isolated from fresh pork sausages packaged in different atmospheres and kept under refrigeration. | the occurrence of in vitro amino acid activity in bacterial strains associated with fresh pork sausages packaged in different atmospheres and kept in refrigeration was studied. the presence of biogenic amines in decarboxylase broth was confirmed by ion-exchange chromatography and by the presence of the corresponding decarboxylase genes by pcr. from the 93 lactic acid bacteria and 100 enterobacteria strains analysed, the decarboxylase medium underestimates the number of biogenic amine-producer st ... | 2011 | 21316866 |
| effects of three heavy metals on the bacteria growth kinetics: a bivariate model for toxicological assessment. | the effects of three heavy metals (co, ni and cd) on the growth kinetics of five bacterial strains with different characteristics (pseudomonas sp., phaeobacter sp. strain 27-4, listonella anguillarum, carnobacterium piscicola and leuconostoc mesenteroides subsp. lysis) were studied in a batch system. a bivariate model, function of time and dose, is proposed to describe simultaneously all the kinetic profiles obtained by incubating a microorganism at increasing concentrations of individual metals ... | 2011 | 21360150 |
| use of carnobacterium maltaromaticum cultures and hydroalcoholic extract of lippia sidoides cham. against listeria monocytogenes in fish model systems. | minimally processed refrigerated ready-to-eat fishes may offer health risk of severe infection to susceptible individuals due to contamination by the psychrotolerant bacterium l. monocytogenes. in this work, inhibition of l. monocytogenes by a plant extract and lactic acid bacteria (lab) was studied in model fish systems kept at 5 °c for 35 days. for that, fillets of tropical fish "surubim" (pseudoplatystoma sp.) and hydroalcoholic extract of the plant lippia sidoides cham. ("alecrim pimenta") w ... | 2011 | 21419508 |
| gluconeotrehalose is the principal organic solute in the psychrotolerant bacterium carnobacterium strain 17-4. | a high proportion of microorganisms that colonise cold environments originate from marine sites; hence, they must combine adaptation to low temperature with osmoregulation. however, little or nothing is known about the nature of compatible solutes used by cold-adapted organisms to balance the osmotic pressure of the external medium. we studied the intracellular accumulation of small organic solutes in the arctic isolate carnobacterium strain 17-4 as a function of the growth temperature and the n ... | 2011 | 21509422 |
| sensory characteristics of spoilage and volatile compounds associated with bacteria isolated from cooked and peeled tropical shrimps using spme-gc-ms analysis. | the spoilage potential of six bacterial species isolated from cooked and peeled tropical shrimps (brochothrix thermosphacta, serratia liquefaciens-like, carnobacterium maltaromaticum, carnobacterium divergens, carnobacterium alterfunditum-like and vagococcus penaei sp. nov.) was evaluated. the bacteria were inoculated into shrimps, packaged in a modified atmosphere and stored for 27days at 8°c. twice a week, microbial growth, as well as chemical and sensory changes, were monitored during the sto ... | 2011 | 21531471 |
| complete genome sequence of carnobacterium sp. 17-4. | members of the carnobacteria have been extensively studied as probiotic cultures in aquacultures and protective cultures in seafood, diary and meat. we report on the finished genome sequence of carnobacterium sp. 17-4, which has been isolated from permanently cold seawater. the genetic information reveals a new circular bacteriocin biosynthesis cluster. | 2011 | 21551290 |
| characterisation of the staphylococcal bacteriophage lysin chap(k). | aims: to develop an efficient purification strategy for the bacteriophage lysin chap(k) . to evaluate its antibacterial spectrum(,) enzymatic properties, optimal reaction conditions and lytic activity against live s. aureus. methods and results: recombinant chap(k) was purified to homogeneity by cation exchange chromatography, with yields of up to 10 mg from 1 litre of e. coli culture. the lytic spectrum of chap(k) includes all staphylococcal species and also members of the genera micrcococcus ... | 2011 | 21812876 |
| microbial metabolites and bacterial diversity in beef stored in different packaging conditions monitored by pyrosequencing, pcr-dgge, spme-gc/ms and 1hnmr. | beef chops were stored at 4°c in different conditions: air (a); modified atmosphere packaging (map); vacuum (v); bacteriocin-activated antimicrobial packaging (av). after 0 to 45 days of storage, analyses were performed to determine loads of spoilage microorganisms, microbial metabolites by spme-gc/ms and (1)hnmr, microbial diversity by pcr-dgge and pyrosequencing. the microbiological shelf-life of meat increased with increasing selectivity of storage conditions. the culture-independent analysis ... | 2011 | 21803905 |
| spoilage-related activity of carnobacterium maltaromaticum strains in air-stored and vacuum-packed meat. | one hundred and three isolates of carnobacterium spp. from raw meat were analyzed by rapd-pcr and identified by 16s rrna gene sequencing. forty-five strains of c. maltaromaticum were characterized for their growth capability at different temperatures, nacl concentrations and ph values and for in vitro lipolytic and proteolytic activities. moreover, their spoilage potential in meat was investigated by analyzing the release of volatile organic compounds (vocs) in meat stored in air or vacuum pack. ... | 2011 | 21784913 |
| the genome sequence of the lactic acid bacterium, carnobacterium maltaromaticum atcc 35586 encodes potential virulence factors. | the genus carnobacterium belongs to the lactic acid bacteria and carnobacterium maltaromaticum is commonly found in modified atmosphere packed and vacuum packed fish and meat products as well as in live fish. this species has been described as a fish pathogenic organism but human clinical isolates have only been obtained at one occasion. to investigate the virulence potential we sequenced the entire genome of strain atcc 35586, isolated from a diseased salmon. when comparing the translated gene ... | 2011 | 21704418 |
| The spoilage of air-packaged broiler meat during storage at normal and fluctuating storage temperatures. | Bacterial diversity and the major flora present on air-packaged broiler meat during storage at normal (4°C) and fluctuating storage temperatures (0-4°C and 4-10°C) were investigated using culture-dependent and culture-independent approaches. Culture-dependent analysis revealed that the growth of microflora was retarded when broiler meat was stored at lower temperatures (0-4°C). Denaturing gradient gel electrophoresis profiles showed that Staphylococcus spp., Pseudomonas spp., Acinetobacter spp., ... | 2012 | 22184446 |
| effect of carnobacterium piscicola on aroma formation in sausage mince. | the metabolites from leucine degradation play a key role in sausage aroma. leucine catabolism is particularly important in carnobacterium piscicola. the purpose of the study was to determine the effect of c. piscicola inoculation on the aroma of a sausage model and on leucine catabolism. sensory analysis and implantation of carnobacterium were performed. aromatic compound production from (3)h leucine added to the sausage model was measured by high-performance liquid chromatography. after 18 days ... | 2003 | 22062397 |
| evaluation of toxic effects of several carboxylic acids on bacterial growth by toxicodynamic modelling. | effects of organic acids on microbial fermentation are commonly tested in investigations about metabolic behaviour of bacteria. however, they typically provide only descriptive information without modelling the influence of acid concentrations on bacterial kinetics. | 2011 | 22118421 |
| Bacterial infections of Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to gamete collecting weirs in Michigan. | Herein, we describe the prevalence of bacterial infections in Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to spawn in two tributaries within the Lake Michigan watershed. Ten bacterial genera, including Renibacterium, Aeromonas, Carnobacterium, Serratia, Proteus, Pseudomonas, Hafnia, Salmonella, Shewanella and Morganella, were detected in the kidneys of Chinook salmon (n = 480) using culture, serological and molecular analyses. Among these, Aeromonas salmonicida was detected at ... | 2012 | 22168454 |
| Study of the microbial ecology of wild and aquacultured Tunisian fresh fish. | Eighty samples of fresh fish were collected in Tunisia and analyzed for microbial load. Quality and hygienic safety of the meat and intestines of wild and aquacultured fresh fish were determined. The mesophilic aerobic plate count and populations of psychrotrophic lactic acid bacteria (LAB) and other psychrotrophic bacteria ranged from 5.67 to 7.29, 4.51 to 6, and 5.07 to 6.21 log CFU/g, respectively. For all microbiological determinations, bacterial counts were lower in meat than in the intest ... | 2011 | 22004828 |
| the culturable intestinal microbiota of triploid and diploid juvenile atlantic salmon (salmo salar) - a comparison of composition and drug resistance. | with the increased use of ploidy manipulation in aquaculture and fisheries management this investigation aimed to determine whether triploidy influences culturable intestinal microbiota composition and bacterial drug resistance in atlantic salmon (salmo salar). the results could provide answers to some of the physiological differences observed between triploid and diploid fish, especially in terms of fish health. | 2011 | 22094054 |
| novel combinations of chitosan, carnocin and sulphite for the preservation of chilled pork sausages. | the aim of this study was to develop novel preservation systems for fresh pork sausages based on combinations of chitosan (polymeric ß-1,4-n-acetylglucosamine) carnocin (a bacteriocin produced by carnobacterium piscicola) and low concentrations of sulphite. two pilot-scale trials showed that 0.6% chitosan combined with low sulphite (170 ppm) retarded the growth of spoilage organisms more effectively (3-4 log cfu/g) than high levels (340 ppm) of sulphite alone at 4 °c for up to 24 days. microbial ... | 2002 | 22061408 |
| rapid species identification of seafood spoilage and pathogenic gram-positive bacteria by maldi-tof mass fingerprinting. | the rapid identification of food pathogenic and spoilage bacteria is important to ensure food quality and safety. seafood contaminated with pathogenic bacteria is one of the major causes of food intoxications, and the rapid spoilage of seafood products results in high economic losses. in this study, a collection of the main seafood pathogenic and spoilage gram-positive bacteria was compiled, including bacillus spp., listeria spp., clostridium spp., staphylococcus spp. and carnobacterium spp. the ... | 2011 | 22009363 |
| tyramine production by a strain of carnobacterium divergens inoculated in meat-fat mixture. | this study determined if a strain of carnobacterium divergens producing tyramine in laboratory medium is able to produce this amine in meat. thus the strain was inoculated into a sterile meat-fat mixture of initial ph 5.3 or 4.9 and incubated at 15°c or 25°c. amine production in these samples was measured at 12 days and compared to that in non-inoculated samples. non-inoculated samples and those inoculated with c. divergens had the same low content of tryptamine, putrescine, cadaverine, histamin ... | 1999 | 22062144 |