| repellency and toxicity of aromatic plant extracts against the mosquito culex pipiens molestus (diptera: culicidae). | the insecticidal activities of essential oil extracts from leaves, flowers and roots of aromatic plants against fourth-instar larvae of the mosquito culex pipiens molestus forskal were determined. extracts of foeniculum vulgare mill were the most toxic, followed by those of ferula hermonis boiss, citrus sinensis osbeck, pinus pinea l, laurus nobilis l and eucalyptus spp with lc50 values of 24.5, 44.0, 60.0, 75.0, 117.0 and 120.0 mg litre(-1), respectively. combination tests between the lc50 and ... | 2005 | 15662650 |
| xiphidorus amazonensis n. sp. (nematoda: longidoridae) from the brazilian amazon basin. | xiphidorus amazonensis n. sp. was found in the rhizospheres of jatropha curcas, musa sp., anona muricata, cassia tora, panicum laxum, paspalum fasciculatum, aeschynomene sensitiva, saccharum officinarum, manihot esculenta, abelmoschus esculentus, tamarindus indica, mangifera indica, vigna unguiculata, zea mays, commelina sp., cyperus rotundus, fimbristylis miliacea, citrus sinensis, and eichhornia crassipes on the amazon river island of xiborena, approximately 40 km southeast of manaus, capital ... | 1985 | 19294098 |
| variation in radopholus citrophilus population densities in the citrus rootstock carrizo citrange. | seedlings of the hybrid citrus rootstock, carrizo citrange (citrus sinensis x poncirus trifoliata) do not uniformly limit development of the citrus burrowing nematode, radopholus citrophilus. variation in nematode population densities in roots of seedlings germinating from the same seed suggests that factors responsible for nematode incompatibility are not functional or are not inherited uniformly among progeny. seeds which produced a single seedling were more likely to produce plants which supp ... | 1986 | 19294135 |
| isolation of xanthyletin, an inhibitor of ants' symbiotic fungus, by high-speed counter-current chromatography. | xanthyletin, an inhibitor of symbiotic fungus (leucoagaricus gongylophorus) of leaf-cutting ant (atta sexdens rubropilosa), as well as suberosin, seselin and xanthoxyletin were isolated from citrus sinensis grafted on citrus limonia. a two-phase solvent system composed of hexane/ethanol/acetonitrile/water (10:8:1:1, v/v) was used for the high-speed counter-current chromatographic isolation of xanthyletin with high yield and over 99% purity as determined by liquid and gas chromatography with mass ... | 2009 | 19296958 |
| in vitro antilisterial effects of citrus oil fractions in combination with organic acids. | the objectives of this study were to screen activity of citrus essential oil fractions (eos) alone and in combination with organic acids against 2 species of listeria. five citrus eos were initially screened by disc diffusion assay for antibacterial activity. cold pressed terpeneless valencia orange oil (cp terpeneless oil) had the strongest bacteriostatic (mic) and bactericidal (mbc) properties at 0.55% and 1.67%, respectively. four organic acids were tested for effectiveness against listeria. ... | 2009 | 19323760 |
| host range and genetic relatedness of colletotrichum acutatum isolates from fruit crops and leatherleaf fern in florida. | isolates of colletotrichum acutatum were collected from anthracnose-affected strawberry, leatherleaf fern, and key lime; ripe-rot-affected blueberry; and postbloom fruit drop (pfd)-affected sweet orange in florida. additional isolates from ripe-rot-affected blueberry were collected from georgia and north carolina and from anthracnose-affected leatherleaf fern in costa rica. pathogenicity tests on blueberry and strawberry fruit; foliage of key lime, leatherleaf fern, and strawberry; and citrus fl ... | 2009 | 19351258 |
| radical scavenging activity of orange and tangerine varieties cultivated in brazil. | four citrus fruit varieties cultivated in brazil (two kinds of sweet orange and two kinds of tangerine) were analyzed for physicochemical characteristics contents of total phenolics, total carotenoids and ascorbic acid, and antioxidant activities. the antioxidant activities of aqueous, methanolic, and acetone extracts of the citrus fruit juices were assessed on the basis of their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (dpph( )). the cravo tangerine has the highest content of citric ac ... | 2009 | 19381992 |
| effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant staphylococcus aureus. | ethanolic extracts and some fractions from 10 indian medicinal plants, known for antibacterial activity, were investigated for their ability to inhibit clinical isolates of beta-lactamase producing methicillin-resistant staphylococcus aureus (mrsa) and methicillin-sensitive s. aureus (mssa). synergistic interaction of plant extracts with certain antibiotics was also evaluated. the mrsa test strains were found to be multi-drug resistant and also exhibited high level of resistance to common beta-l ... | 2005 | 15812867 |
| sequencing and computational analysis of complete genome sequences of citrus yellow mosaic badna virus from acid lime and pummelo. | citrus yellow mosaic badna virus (cmbv), a member of the family caulimoviridae, genus badnavirus, is the causative agent of citrus mosaic disease in india. although the virus has been detected in several citrus species, only two full-length genomes, one each from sweet orange and rangpur lime, are available in publicly accessible databases. in order to obtain a better understanding of the genetic variability of the virus in other citrus mosaic-affected citrus species, we performed the cloning an ... | 2009 | 19444599 |
| construction and characterization of two citrus bac libraries and identification of clones containing the phytoene synthase gene. | two deep-coverage bacterial artificial chromosome (bac) libraries of citrus sinensis (l.) osbeck 'cara cara' navel orange and citrus reticulata (l.) blanco 'egan no. 1' ponkan mandarin, which belong to the two most important species of the citrus genus, have been constructed and characterized to facilitate gene cloning and to analyze variety-specific genome composition. the c. sinensis bac library consists of 36 000 clones with negligible false-positive clones and an estimated average insert siz ... | 2009 | 19448728 |
| pathotypes and genetic relationship of worldwide collections of elsinoë spp. causing scab diseases of citrus. | two scab diseases are recognized currently on citrus: citrus scab, caused by elsinoë fawcettii, and sweet orange scab, caused by e. australis. because the two species cannot be reliably distinguished by morphological or cultural characteristics, host range and molecular methods must be used to identify isolates. four pathotypes of e. fawcettii and two of e. australis have been described to date based on host range. the host specificity and genetic relationships among 76 isolates from argentina, ... | 2009 | 19453232 |
| enantiomeric composition of (3r)-(-)- and (3s)-(+)-linalool in various essential oils of indian origin by enantioselective capillary gas chromatography-flame ionization and mass spectrometry detection methods. | enantiomeric ratios of linalool have been determined in various authentic essential oils of indian origin using 10% heptakis(2,3-di-o-methyl-6-o-tert-butyldimethylsilyl)-beta-cyclodextrin as a chiral stationary phase. a complete enantiomeric excess (ee) for (3s)-(+)-linalool was characteristic of lippia alba and cinnamomum tamala leaf oils while less than 90% excess was noticed in zanthoxylum armatum leaf, zingiber roseum root/rhizome and citrus sinensis leaf oils. on the contrary, an enantiomer ... | 2009 | 19476006 |
| toward validation of process criteria for high-pressure processing of orange juice with predictive models. | mathematical models were developed to predict time to inactivation (tti) by high-pressure processing of salmonella in australian valencia orange juice (ph 4.3) and navel orange juice (ph 3.7) as a function of pressure magnitude (300 to 600 mpa) and inoculum level (3 to 7 log cfu/ml). for each model, the tti was found to increase with increasing inoculum level and decrease with increasing pressure magnitude. the u.s. food and drug administration juice hazard analysis and critical control point re ... | 2005 | 15895726 |
| comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics. | to compare the bacteriostatic and bactericidal activity of 13 chemotyped essential oils (eo) on 65 bacteria with varying sensitivity to antibiotics. | 2008 | 19552780 |
| intraplantar injection of bergamot essential oil into the mouse hindpaw: effects on capsaicin-induced nociceptive behaviors. | despite the increasing use of aromatherapy oils, there have not been many studies exploring the biological activities of bergamot (citrus bergamia, risso) essential oil (beo). recently, we have investigated the effects of beo injected into the plantar surface of the hindpaw in the capsaicin test in mice. the intraplantar injection of capsaicin produced an intense and short-lived licking/biting response toward the injected hindpaw. the capsaicin-induced nociceptive response was reduced significan ... | 2009 | 19607974 |
| a rapid liquid chromatography electrospray ionization mass spectrometry(n) method for evaluation of synephrine in citrus aurantium l. samples. | immature bitter orange fruit and its extracts have been introduced into the market as an alternative to ephedra in weight loss products. however, the safety of the immature bitter orange fruit and its extracts is a debated argument due to the presence of synephrine, a constituent known as a sympathomimetic agent. in this paper, we describe the development of a new, rapid, and simple liquid chromatography-electrospray ionization-tandem mass spectrometry method devoted to the quantitative determin ... | 2005 | 16366666 |
| calibration of sap flow estimated by the compensation heat pulse method in olive, plum and orange trees: relationships with xylem anatomy. | the compensation heat pulse method is widely used to estimate sap flow in conducting organs of woody plants. being an invasive technique, calibration is crucial to derive correction factors for accurately estimating the sap flow value from the measured heat pulse velocity. we compared the results of excision and perfusion calibration experiments made with mature olive (olea europaea l. 'manzanilla de sevilla'), plum (prunus domestica l. 'songal') and orange (citrus sinensis (l.) osbeck. 'cadener ... | 2006 | 16510387 |
| transmission of different isolates of spiroplasma citri to carrot and citrus by circulifer tenellus (hemiptera: cicadellidae). | carrot purple leaf disease was first reported in 2006 in the state of washington and was associated with spiroplasma citri. the disease also was reported in california in 2008. the objectives of this work were to fulfill koch's postulates and to determine 1) whether the beet leafhopper, circulifer tenellus (baker) (hemiptera: cicadellidae), transmits carrot [daucus carota l. subsp. sativus (hoffm.) arcang] isolates of s. citri; and 2) whether carrot and citrus [citrus sinensis (l.) osb.]-derived ... | 2009 | 19736751 |
| gene expression analysis in citrus reveals the role of gibberellins on photosynthesis and stress. | the effect of gibberellins (ga) on internode transcriptome was investigated in transgenic carrizo citrange (citrus sinensis x poncirus trifoliata) plants overexpressing endogenous ccga20ox1 (encoding a ga biosynthetic gene), and in non-transformed explants treated with ga(3), using a citrus cdna microarray. substantial modulation of gene expression was found in sense ccga20ox plants. extensive up-regulation of genes involved in photosynthesis and carbon utilization, and down-regulation of those ... | 2008 | 18684239 |
| a comparison of citrus blossom volatiles. | the objective of this study was to identify the major volatiles and their relative concentrations in intact grapefruit, sweet orange, sour orange, mandarin, lemon, lime and pummelo blossoms. volatiles from freshly picked blossoms were collected and concentrated using static headspace solid-phase microextraction and then separated and identified using gc-ms. seventy volatiles were detected, 66 identified, of which 29 were identified for the first time in citrus blossoms. major volatiles consisted ... | 2009 | 19747702 |
| dispersion patterns and sampling plans for diaphorina citri (hemiptera: psyllidae) in citrus. | the abundance and spatial dispersion of diaphorina citri kuwayama (hemiptera: psyllidae) were studied in 34 grapefruit (citrus paradisi macfad.) and six sweet orange [citrus sinensis (l.) osbeck] orchards from march to august 2006 when the pest is more abundant in southern texas. although flush shoot infestation levels did not vary with host plant species, densities of d. citri eggs, nymphs, and adults were significantly higher on sweet orange than on grapefruit. d. citri immatures also were fou ... | 2008 | 18767763 |
| antidiabetic potential of citrus sinensis and punica granatum peel extracts in alloxan treated male mice. | an investigation on the effects of four different concentrations of peel extract from citrus sinensis (cs) or punica granatum (pg) in male mice revealed the maximum glucose lowering and antiperoxidative activities at 25 mg/kg of cs and 200 mg/kg of pg. in a separate experiment their potential was evaluated with respect to the regulation of alloxan induced diabetes mellitus. while a single dose of alloxan (120 mg/kg) increased the serum levels of glucose and alpha-amylase activity, rate of water ... | 2007 | 18806305 |
| diversity of endophytic yeasts from sweet orange and their localization by scanning electron microscopy. | endophytes are microorganisms that colonize plant tissues internally without causing harm to the host. despite the increasing number of studies on sweet orange pathogens and endophytes, yeast has not been described as a sweet orange endophyte. in the present study, endophytic yeasts were isolated from sweet orange plants and identified by sequencing of internal transcribed spacer (its) rrna. plants sampled from four different sites in the state of são paulo, brazil exhibited different levels of ... | 2009 | 19798655 |
| inheritance and heritability of resistance to citrus leprosis. | abstract the genetic inheritance of resistance to leprosis, the most important viral disease of citrus in brazil, was characterized through the phenotypic assessment of 143 hybrids resulting from crosses between tangor 'murcott' (citrus sinensis x c. reticulata) and sweet orange 'pêra' (c. sinensis), considered to be resistant and susceptible to the disease, respectively. all plants were grafted onto rangpur lime (c. limonia) and inoculated with citrus leprosis virus, cytoplasmic type through th ... | 2006 | 18943497 |
| functional characterization of citrus psy gene in hongkong kumquat (fortunella hindsii swingle). | citrus, rich in carotenoids, is the most important fruit crop based on the total annual production. in the carotenoid biosynthesis pathway, phytoene synthase (psy, ec 2.5.1.32) catalyzes the dimerization of two molecules of geranylgeranyl pyrophosphate (ggpp) to phytoene and has been shown to be a rate-limiting enzyme for the synthesis of carotenoids. in this study, we investigated catalytic activity of cspsy from cara cara navel orange (citrus sinensis osbeck) by heterologous expression in esch ... | 2009 | 19813015 |
| essential oils and anxiolytic aromatherapy. | a number of essential oils are currently in use as aromatherapy agents to relieve anxiety, stress, and depression. popular anxiolytic oils include lavender (lavandula angustifolia), rose (rosa damascena), orange (citrus sinensis), bergamot (citrus aurantium), lemon (citrus limon), sandalwood (santalum album), clary sage (salvia sclarea), roman chamomile (anthemis nobilis), and rose-scented geranium (pelargonium spp.). this review discusses the chemical constituents and cns effects of these aroma ... | 2009 | 19831048 |
| comparative transcripts profiling reveals new insight into molecular processes regulating lycopene accumulation in a sweet orange (citrus sinensis) red-flesh mutant. | interest in lycopene metabolism and regulation is growing rapidly because accumulative studies have suggested an important role for lycopene in human health promotion. however, little is known about the molecular processes regulating lycopene accumulation in fruits other than tomato so far. | 2009 | 19922663 |
| different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione s-transferases. | glutathione s-transferases (gsts) catalyze the conjugation of glutathione to hydrophobic compounds, contributing to the metabolism of toxic chemicals. in this study, we show that two naturally occurring tau gsts (gstus) exhibit distinctive kinetic parameters towards 1-chloro-2,4-dinitrobenzene (cdnb), although they differ only in three amino acids (arg89, glu117 and ile172 in gstu1 are replaced by pro89, lys117 and val172 in gstu2). in order to understand the effects of the single mismatched res ... | 2010 | 19954490 |
| detection of huanglongbing (citrus greening) disease by nucleic acid spot hybridization. | polymerase chain reaction (pcr) amplification with primers specific to the rdna region successfully amplified the 1160-bp dna fragment from a huanglongbing (hlb)-infected sweet orange sample with mottling symptoms leaves, but not from healthy sweet orange plants. the pcr product of 1160-bp was used as probe labeled with biotin for detection of the hlb pathogen in the nucleic acid spot hybridization (nash) test. it was found that the hlb pathogen could be detected up to 1:100 dilution in hlb-infe ... | 2009 | 19957441 |
| blood orange juice inhibits fat accumulation in mice. | to analyze the effect of the juice obtained from two varieties of sweet orange (citrus sinensis l. osbeck), moro (a blood orange) and navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (hfd). | 2010 | 20029381 |
| genetic diversity of xylella fastidiosa strains from costa rica, são paulo, brazil, and united states. | abstract the diversity of 42 xylella fastidiosa strains from costa rica, são paulo, brazil, and the united states were analyzed using the sequence of the 16s rrna gene by variable number of tandem repeat (vntr) fragment analysis and by restriction fragment length polymorphisms (rflp) of a specific polymerase chain reaction (pcr)-amplification product using enzyme cfoi. limited variability in the sequence of the 16s rrna gene was observed and, although the separation was not absolute, most strain ... | 2007 | 18943693 |
| [survey of sharpshooters (hemiptera: cicadellidae) associated with xylella fastidiosa transmission in citrus groves of the north coast of bahia state]. | the causal agent of citrus variegated clorosis, xylella fastidiosa, is transmitted by leafhoppers of the subfamily cicadellinae, whose species vary regionally. the goal of this study was to identify potential vectors of this pathogen in citrus groves of bahia north coast, brazil. the survey was done from march/2002 to february/2003 in three seven- to nine-year-old sweet orange (citrus sinensis, pêra variety) groves located in rio real, ba. fifteen yellow sticky cards (8.5x11.5 cm) were installed ... | 2009 | 20098930 |
| [diversity of hemiptera auchenorrhyncha in citrus, coffee and a fragment of native forest of the state of são paulo]. | the population of hemiptera auchenorrhyncha was studied in sweet citrus groves (citrus sinensis), coffee plantations (coffea arabica) and a semi-deciduous seasonal forest with shrub physiognomy in bebedouro, sp, to evaluate the influence of the natural ecosystem on the species composition of the agroecosystems. monitoring was carried out by using yellow stick cards, which were replaced every 15 days and all auchenorrhyncha collected were counted and identified. seven families, 11 subfamilies and ... | 2009 | 20098931 |
| a phytoplasma closely related to the pigeon pea witches'-broom phytoplasma (16sr ix) is associated with citrus huanglongbing symptoms in the state of são paulo, brazil. | in february 2007, sweet orange trees with characteristic symptoms of huanglongbing (hlb) were encountered in a region of são paulo state (sps) hitherto free of hlb. these trees tested negative for the three liberibacter species associated with hlb. a polymerase chain reaction (pcr) product from symptomatic fruit columella dna amplifications with universal primers fd1/rp1 was cloned and sequenced. the corresponding agent was found to have highest 16s rdna sequence identity (99%) with the pigeon p ... | 2008 | 18943735 |
| a comparison of volatile components of setomi with its parent cultivars. | the citrus fruit setomi is a hybrid of yoshiura ponkan (citrus reticulate blanco) and kiyomi (citrus unshiu mrcov. x citrus sinensis osbeck). the essential oils from the peel of setomi and its parent cultivars were obtained by a simultaneous distillation extraction technique. comparing the essential oils of setomi and its parent cultivars, it was found that the oil in the peel of setomi consisted of characteristic aroma components from each parent cultivar. the principal component analysis of da ... | 2010 | 20208376 |
| anxiolytic-like effect of sweet orange aroma in wistar rats. | aromatherapy is the use of essential oils as an alternative treatment for medical purposes. despite the lack of sufficient scientific proof, it is considered a holistic complementary therapy employed to enhance comfort and decrease distress. citrus fragrances have been particularly used by aromatherapists for the treatment of anxiety symptoms. based on this claim, the present study investigated the effects of citrus sinensis (sweet orange) essential oil on wistar, male rats evaluated in the elev ... | 2010 | 20211673 |
| host-pathogen-biocontrol agent interaction as affected by sequential application of na2co3 and cacl2. | among the alternatives to synthetic postharvest fungicides encouraging results have been reported with biocontrol agents, and on citrus fruits, their efficacy was improved when co-applied with gras compounds or with physical means. still, the reason for this increased efficacy has not been explained and therefore a study was performed using orange fruit (citrus sinensis osbec. cv 'washington navel') as host, p. digitatum as the pathogen, a yeast (pichia guiliermondii, isolate 5a) as the biocontr ... | 2009 | 20222553 |
| microbial inhibitory and radical scavenging activities of cold-pressed terpeneless valencia orange (citrus sinensis) oil in different dispersing agents. | due to their low solubility in water, oil-based bioactive compounds require dispersion in a surface-active agent or appropriate solvents to ensure maximum contact with microorganisms. these combinations, however, may change their physical and/or chemical characteristics and consequently alter the desired functionality. the objective of this study was to determine the impact of selected dispersing agents, ethanol, dimethyl sulfoxide (dmso), and tween-80, on cold-pressed terpeneless (cpt) valencia ... | 2010 | 20355124 |
| chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of citrus maxima burm. and citrus sinensis (l.) osbeck essential oils and their cyclic monoterpene, dl-limonene. | the study deals with antifungal, antiaflatoxigenic and antioxidant activity of citrus maxima and citrus sinensis essential oils (eos) and their phytochemical composition. the eos were obtained by hydrodistillation and their chemical profile was determined through gc and gc-ms analysis. both the eos and their 1:1 combination showed broad fungitoxic spectrum against different food contaminating moulds. the eos and their combination completely inhibited aflatoxin b(1) (afb(1)) production at 500 ppm ... | 2010 | 20385194 |
| the effects of host, geographic origin, and gender on the thermal requirements of diaphorina citri (hemiptera: psyllidae). | diaphorina citri kuwayama (hemiptera: psyllidae) is the vector of the bacteria that causes citrus greening and is considered one of the world's most important citrus diseases. we examined how host, geographic region, and gender affect the thermal requirements of d. citri. the insects were reared in climatic chambers at constant temperatures of 18, 20, 22, 25, 28, 30, and 32 +/- 1 degrees c, 70 +/- 10% rh, and a 14 h photophase. host plants for d. citri included orange (citrus sinensis [rutaceae] ... | 2010 | 20388302 |
| presence of xylella fastidiosa in sweet orange fruit and seeds and its transmission to seedlings. | abstract xylella fastidiosa, a xylem-limited bacterium, causes several economically important diseases in north, central, and south america. these diseases are transmitted by sharpshooter insects, contaminated budwood, and natural root-grafts. x. fastidiosa extensively colonizes the xylem vessels of susceptible plants. citrus fruit have a well-developed vascular system, which is continuous with the vascular system of the plant. citrus seeds develop very prominent vascular bundles, which are atta ... | 2003 | 18943861 |
| stereoselective conjugation, transport and bioactivity of s- and r-hesperetin enantiomers in vitro. | the flavanone hesperetin ((+/-)-4'-methoxy-3',5,7-trihydroxyflavanone) is the aglycone of hesperidin, which is the major flavonoid present in sweet oranges. hesperetin contains a chiral c-atom and so can exist as an s- and r-enantiomer, however, in nature 2s-hesperidin and its s-hesperetin aglycone are predominant. the present study reports a chiral hplc method to separate s- and r-hesperetin on an analytical and semipreparative scale. this allowed characterization of the stereoselective differe ... | 2010 | 20441166 |
| an evaluation of the genetic diversity of xylella fastidiosa isolated from diseased citrus and coffee in são paulo, brazil. | abstract strains of xylella fastidiosa, isolated from sweet orange trees (citrus sinensis) and coffee trees (coffea arabica) with symptoms of citrus variegated chlorosis and requeima do café, respectively, were indistinguishable based on repetitive extragenic palindromic polymerase chain reaction (pcr) and enterobacterial repetitive intergenic consensus pcr assays. these strains were also indistinguishable with a previously described pcr assay that distinguished the citrus strains from all other ... | 2001 | 18943950 |
| glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation. | phospholipid hydroperoxide glutathione peroxidase (phgpx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. to study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (citrus sinensis) phgpx (cit-phgpx). all attempts to obtain regenerated plants expressing this enzyme constitutively failed. however, when the enzyme's catalytic activity was abolished by active sit ... | 2010 | 20530511 |
| effect of liberibacter infection (huanglongbing or "greening" disease) of citrus on orange juice flavor quality by sensory evaluation. | some anecdotal reports suggest that infection of citrus trees with candidatus liberibacter asiaticus (las), the suspected causal agent of huanglongbing (hlb) disease, imparts off flavor to orange juice. it is of interest to the industry to know how las infection affects juice quality with respect to cultivar, maturity, or processing method. hamlin, midsweet, and valencia oranges were harvested over 2 y from trees that tested negative (las-) or positive (las+) for las from different groves and in ... | 2010 | 20546425 |
| evaluation of in vitro activity of essential oils against trypanosoma brucei brucei and trypanosoma evansi. | essential oils (eos) from cymbopogon citratus (cc), eucalyptus citriodora (ec), eucalyptus camaldulensis (ed), and citrus sinensis (cs) were obtained by hydrodistillation process. the eos were evaluated in vitro for activity against trypanosoma brucei brucei (tbb) and trypanosoma evansi (t. evansi). the eos were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. the drop in number of parasite over time was achieved doses of 0.4 g/ml, 0.2 g/ ... | 2010 | 20700425 |
| postbloom fruit drop of citrus and key lime anthracnose are caused by distinct phylogenetic lineages of colletotrichum acutatum. | colletotrichum acutatum causes two diseases of citrus, postbloom fruit drop (pfd) and key lime anthracnose (kla). pfd is a disease restricted to flowers of sweet orange and most other citrus, and symptoms include petal necrosis, abscission of developing fruit, and the formation of persistent calyces. kla is a disease of foliage, flowers, and fruits of key lime only, and symptoms include necrotic lesions on leaves, fruits, twigs, flowers, and blight of entire shoots. the internal transcribed spac ... | 2008 | 18944086 |
| geographical genetic structure of xylella fastidiosa from citrus in são paulo state, brazil. | abstract a total of 360 xylella fastidiosa strains were isolated from sweet orange (citrus sinensis) cv. pera plants growing in five geographic regions in the brazilian state of são paulo. the genetic variation of these strains was determined by 15 variable number tandem repeat (vntr) and 58 random amplified polymorphic dna (rapd) markers. the mean values of genetic diversity (h) of x. fastidiosa strains within each geographic region determined by rapd (h(rapd)) were substantially lower than h(v ... | 2003 | 18944153 |
| shedding light on the role of photosynthesis in pathogen colonization and host defense. | the role of photosynthesis in plant defense is a fundamental question awaiting further molecular and physiological elucidation. to this end we investigated host responses to infection with the bacterial pathogen xanthomonas axonopodis pv. citri, the pathogen responsible for citrus canker. this pathogen encodes a plant-like natriuretic peptide (xacpnp) that is expressed specifically during the infection process and prevents deterioration of the physiological condition of the infected tissue. prot ... | 2010 | 20798833 |
| quantification of xylella fastidiosa from citrus trees by real-time polymerase chain reaction assay. | abstract xylella fastidiosa is the causal agent of citrus variegated chlorosis (cvc), a destructive disease of sweet orange cultivars in brazil. polymerase chain reaction (pcr)-based assays constitute the principal diagnostic method for detection of these bacteria. in this work, we established a real-time quantitative pcr (qpcr) assay to quantify x. fastidiosa in naturally and artificially infected citrus. the x. fastidiosa cell number detected in the leaves increased according to the age of the ... | 2002 | 18944214 |
| juice components and antioxidant capacity of four tunisian citrus varieties. | juices from four citrus species of tunisia were investigated mainly for quality parameters and antioxidant capacity. | 2011 | 20862741 |
| spatial and temporal analyses of citrus sudden death as a tool to generate hypotheses concerning its etiology. | abstract citrus sudden death (csd), a new disease of unknown etiology that affects sweet orange grafted on rangpur lime, was visually monitored for 14 months in 41 groves in brazil. ordinary runs analysis of csd-symptomatic trees indicated a departure from randomness of symptomatic trees status among immediately adjacent trees mainly within rows. the binomial index of dispersion (d) and the intraclass correlation (k) for various quadrat sizes suggested aggregation of csd-symptomatic trees for al ... | 2003 | 18944366 |
| response of sweet orange (citrus sinensis) to 'candidatus liberibacter asiaticus' infection: microscopy and microarray analyses. | citrus greening or huanglongbing (hlb) is a devastating disease of citrus. hlb is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium 'candidatus liberibacter spp.' in this report, we used sweet orange (citrus sinensis) leaf tissue infected with 'ca. liberibacter asiaticus' and compared this with healthy controls. investigation of the host response was examined with citrus microarray hybridization based on 33,879 expressed sequence tag sequences from several citrus sp ... | 2009 | 19055434 |
| effect of chlorine dioxide gas on salmonella enterica inoculated on navel orange surfaces and its impact on the quality attributes of treated oranges. | microorganisms, including pathogens of public health significance, have been shown to contaminate orange juice during the mechanical extraction of juice. the problem gets exacerbated when washed oranges have high initial microbial load, due to an insufficient postharvest treatment. the objective of this study was to investigate the reduction of salmonella enterica on orange surfaces using clo₂ gas treatments to achieve a 5 log reduction, consistent with the recommendations of the u.s. department ... | 2010 | 20932090 |
| monodemethylated polymethoxyflavones from sweet orange (citrus sinensis) peel inhibit growth of human lung cancer cells by apoptosis. | polymethoxyflavones (pmfs) are almost exclusively found in the citrus genus, particularly in the peels of sweet orange (citrus sinensis l. osbeck) and mandarin (c. reticulate blanco). we studied the effects of two major pmfs, namely, nobiletin and 3,5,6,7,8,3',4'-heptamethoxyflavone (hmf), and two major monodemethylated pmfs, namely 5-hydroxy-3,7,8,3',4'-pentamethoxyflavone (5hpmf), and 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5hhmf), on the growth of human lung cancer h1299, h441, and h460 c ... | 2009 | 19065586 |
| transformation of sweet orange [citrus sinensis (l.) osbeck] with ptha-nls for acquiring resistance to citrus canker disease. | the cooh terminal of ptha encoding three nuclear localizing signals (nls) was amplified by polymerase chain reaction (pcr) from the plasmid of xanthomonas axonopodis pv. citri, the pathogen of citrus canker disease. then the sense and antisense strands of the nls were cloned into pbi121 vector. ptha-nls driven by the camv35 s promoter was transferred into sweet orange via agrobacterium -mediated transformation. successful integration was confirmed by pcr and southern blotting, and 12 sense-nls ( ... | 2010 | 20972821 |
| effective sampling range of a synthetic protein-based attractant for ceratitis capitata (diptera: tephritidae). | studies were conducted in honduras to determine effective sampling range of a female-targeted protein-based synthetic attractant for the mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae). multilure traps were baited with ammonium acetate, putrescine, and trimethylamine lures (three-component attractant) and sampled over eight consecutive weeks. field design consisted of 38 traps (over 0.5 ha) placed in a combination of standard and high-density grids to facilitate ge ... | 2010 | 21061993 |
| antiplasmodial activity of botanical extracts against plasmodium falciparum. | the absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. the aim of the study was to discover effective plant extracts against plasmodium falciparum. in the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of citrus sinensis (peel), leucas aspera, ocimum sanctum, phyllanthus acidus (leaf), terminalia chebula (seed) wer ... | 2010 | 21079994 |
| the effect of pre spring spray to reduce of citrus important pests. | the importance of pre spring spray against citrus aphids, pulvinaria aurantii cockerell and panonychus citri mcgregor that are the most important pest of citrus during spring was tested. in this research, 150 trees ten years old sweet orange (thomson navel on citrus aurantium (root stocks)) in a citrus orchard approximately three hectares sampled. the experiment was laid out in a totally randomized (one-way) design replicated five times. according to the results, the pre spring spray do not effe ... | 2008 | 19137868 |
| quantitative distribution of 'candidatus liberibacter asiaticus' in citrus plants with citrus huanglongbing. | citrus huanglongbing (hlb), or greening disease, is strongly associated with any of three nonculturable gram-negative bacteria belonging to 'candidatus liberibacter spp.' 'ca. liberibacter spp.' are transmitted by citrus psyllids to all commercial cultivars of citrus. the diseases can be lethal to citrus and have recently become widespread in both são paulo, brazil, and florida, united states, the locations of the largest citrus industries in the world. asiatic hlb, the form of the disease found ... | 2009 | 19159305 |
| elsinoë fawcettii and elsinoë australis: the fungal pathogens causing citrus scab. | elsinoë fawcettii and e. australis are important pathogens of citrus. both species are known to produce red or orange pigments, called elsinochrome. elsinochrome is a nonhost-selective phytotoxin and is required for full fungal virulence and lesion formation. this article discusses the taxonomy, epidemiology, genetics and pathology of the pathogens. it also provides a perspective on the cellular toxicity, biosynthetic regulation and pathological role of elsinochrome phytotoxin. taxonomy: elsinoë ... | 2010 | 21199563 |
| pharmacological exploration of the sedative mechanism of hesperidin identified as the active principle of citrus sinensis flowers. | the infusion of flowers of several species of citrus genera is used as a sedative to treat insomnia in mexican traditional medicine. the aims of this study were to investigate the sedative effect of different extracts of flowers of citrus sinensis (l.) osbeck (rutaceae) and describe the pharmacological action mechanism of the sedative active compounds of this plant. the methanol and dichloromethane extracts, obtained from the flowers of citrus sinensis (l.) osbeck (rutaceae), showed a dose-depen ... | 2009 | 19219759 |
| insecticidal activity against bemisia tabaci biotype b of peel essential oil of citrus sinensis var. pear and citrus aurantium cultivated in northeast brazil. | the fumigant action of peel essential oils of citrus sinensis var. pear (pear orange = po) and c. aurantium (bitter orange = bo) from the northeast of brazil were evaluated against bemisia tabaci biotype b and compared with eugenol as a positive control. the oil concentration in the po at 8.5 microl/l of air caused 97% mortality, while the oil concentration of bo at 9.5 microl/l of air caused 99% mortality. however, the lc50 estimates for both oils (lc50 = 3.80 microl/l of air for po and lc50 = ... | 2010 | 21213990 |
| distribution of aroma volatile compounds in tangerine hybrids and proposed inheritance. | with the desirable combination of sugars and acids, volatile compounds contribute to the essential organoleptic attributes of citrus. this study evaluated the aroma volatiles of 20 tangerine hybrids of the university of florida breeding program. volatiles were sampled from hand-squeezed juice by headspace solid-phase microextraction (spme), and analyzed by gas chromatography-mass spectrometry. principal component analysis (pca) and cluster analysis (ca) were used to find similarities among sampl ... | 2010 | 21218478 |
| azteca barbifex forel (hymenoptera: formicidae): potential pest of citrus crops in eastern amazon. | damage caused by the ant azteca barbifex (forel) was identified in orange trees (citrus sinensis), in capitão poço county, guamá microregion, pará state. the damage caused by the scraping of stems and branches lead to reduction in yield with subsequent death of the plant. these characteristics indicate a. barbifex as a potential pest of citrus crops in the eastern region of amazon. | 2010 | 21271082 |
| biomimetic preparation of polymer-supported free radical scavenging, cytocompatible and antimicrobial "green" silver nanoparticles using aqueous extract of citrus sinensis peel. | in the pursuit of making the nanoscale-research greener, the utilization of the reductive potency of a common byproduct of food processing industry i.e. orange peel is reported here to prepare biopolymer-templated "green" silver nanoparticles. aqueous extract of orange peel at basic ph was exploited to prepare starch supported nanoparticles under ambient conditions. the compositional abundance of pectins, flavonoids, ascorbic acid, sugars, carotenoids and myriad other flavones may be envisaged f ... | 2011 | 21316933 |
| selection and validation of reference genes for gene expression studies by reverse transcription quantitative pcr in xanthomonas citri subsp. citri during infection of citrus sinensis. | xanthomonas citri subsp. citri (xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (citrus sinensis (l.) osbeck). the expression of nine candidate internal reference genes was analyzed in xcc grown alone and during c. sinensis infection to identify genes most suitable for comparative expression studies in xcc using reverse transcription quantitative pcr (qrt-pcr). the stability of these genes was determined using the programs genorm, normfinder and bestkeeper. the genes ... | 2011 | 21318633 |
| antioxidant capacity of hesperidin from citrus peel using electron spin resonance and cytotoxic activity against human carcinoma cell lines. | hesperidin is a flavonoid that has various pharmacological activities including anti-inflammatory, antimicrobial and antiviral activities. | 2011 | 21323480 |
| citrus tristeza virus infection induces the accumulation of viral small rnas (21-24-nt) mapping preferentially at the 3'-terminal region of the genomic rna and affects the host small rna profile. | to get an insight into the host rna silencing defense induced by citrus tristeza virus (ctv) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (solexa-illumina) the small rnas (srnas) in three virus-host combinations. our data show that ctv srnas: (i) represent more than 50% of the total srnas in mexican lime and sweet orange (where ctv reaches relatively high titers), but only 3.5% in sour orange (where th ... | 2011 | 21327514 |
| ectopic expression of mdspds1 in sweet orange (citrus sinensis osbeck) reduces canker susceptibility: involvement of hôééoôéé production and transcriptional alteration. | enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene. | 2011 | 21439092 |
| spiroplasmas are the causal agents of citrus little-leaf disease. | a spiroplasma isolated from citrus with little-leaf disease was grown in a cell-free medium and injected into leafhoppers (euscelis plebejus). injected leafhoppers, but not those fed on infected plants, transmitted the spiroplasma to white clover (trifolium repens cv. s100) and sweet orange (citrus sinensis cv. valencia). infected clover plants were severely stunted; infected sweet orange plants showed typical symptoms of citrus little-leaf disease. the spiroplasma was detected in clover and swe ... | 1974 | 19280788 |
| a comparison of four dna extraction methods for the detection of citrus yellow mosaic badna virus from two species of citrus using pcr and dot-blot hybridization. | nucleic acid preparations extracted using four procedures were assessed to determine the suitability of the procedure for pcr-based and dna dot-blot-based detection of citrus yellow mosaic badna virus (cmbv) from two citrus species, acid lime and pummelo. it was found that the success of pcr detection depended upon the procedure of dna extraction whereas the dot-blot detection was successful with all extraction methods examined. cmbv dna sequences amplified from two citrus species indicated high ... | 2008 | 18582956 |
| terpene down-regulation in orange reveals the role of fruit aromas in mediating interactions with insect herbivores and pathogens. | plants use volatile terpene compounds as odor cues for communicating with the environment. fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. we have investigated how terpenes in citrus fruit peels affect the interaction between the plant, insects, and microorganisms. because limonene represents up to 97% of the total volatiles in orange (citrus sinensis) fruit peel, we chose to down-regulate the expression of a limonene synthase gene in ora ... | 2011 | 21525333 |
| biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity. | biosynthesis of silver nanoparticles (agnps) was achieved by a novel, simple green chemistry procedure using citrus sinensis peel extract as a reducing and a capping agent. the effect of temperature on the synthesis of silver nanoparticles was carried out at room temperature (25°c) and 60°c. the successful formation of silver nanoparticles has been confirmed by uv-vis, ftir, xrd, edax, fesem and tem analysis and their antibacterial activity against escherichia coli, pseudomonas aeruginosa (gram- ... | 2011 | 21536485 |
| tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment. | background and aims polyploidy is a major component of plant evolution. the citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. the main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation. methods tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry ... | 2011 | 21586529 |
| the sucrose synthase-1 promoter from citrus sinensis directs expression of the β-glucuronidase reporter gene in phloem tissue and in response to wounding in transgenic plants. | interest in phloem-specific promoters for the engineering of transgenic plants has been increasing in recent years. in this study we isolated two similar, but distinct, alleles of the citrus sinensis sucrose synthase-1 promoter (cssus1p) and inserted them upstream of the β-glucuronidase (gus) gene to test their ability to drive expression in the phloem of transgenic arabidopsis thaliana and nicotiana tabacum. although both promoter variants were capable of conferring localized gus expression in ... | 2011 | 21594624 |
| distribution and biological role of the oligopeptide-binding protein (oppa) in xanthomonas species. | in this study we investigated the prevalence of the oppa gene, encoding the oligopeptide binding protein (oppa) of the major bacterial oligopeptide uptake system (opp), in different species of the genus xanthomonas. the oppa gene was detected in two xanthomonas axonopodis strains among eight tested xanthomonas species. the generation of an isogenic oppa-knockout derivative of the xac 306 strain, showed that the oppa protein neither plays a relevant role in oligopeptide uptake nor contributes to ... | 2010 | 21637492 |
| chemical characterization of citrus sinensis grafted on c. limonia and the effect of some isolated compounds on the growth of xylella fastidiosa. | citrus sinensis grafted on c. limonia produces a considerable number of compounds that are not common in both plants developed from germination of seeds. the chemical profile of scion and rootstock differ notably for absence in the form of flavonoids and coumarins containing c5 prenyl groups attached to the carbon atoms of aromatic and heterocyclic systems or to oxygen. only linear pyranocoumarins xanthyletin and xanthoxyletin were found in scion. this observation indicates that the prenylated c ... | 2008 | 18683948 |
| citrus essential oils and four enantiomeric pinenes against culex pipiens (diptera: culicidae). | the aim of this study was to evaluate the toxicity of pinenes (enantiomers of alpha- and beta-) and essential oils from greek plants of the rutaceae family against the mosquito larvae of culex pipiens (diptera: culicidae). essential oils were isolated by hydrodistillation from fruit peel of orange (citrus sinensis l.), lemon (citrus limon l.), and bitter orange (citrus aurantium l.). the chemical composition was determined by gas chromatography/mass spectrometry (gc/ms) analysis. citrus essentia ... | 2009 | 19424721 |
| colonization of citrus seed coats by 'candidatus liberibacter asiaticus': implications for seed transmission of the bacterium. | huanglongbing is an economically damaging disease of citrus associated with infection by 'ca. liberibacter asiaticus'. transmission of the organism via infection of seeds has not been demonstrated but is a concern since some citrus varieties, particularly those used as rootstocks in commercial plantings are propagated from seed. we compared the incidence of detection of 'ca. liberibacter asiaticus' dna in individual fruit peduncles, seed coats, seeds and in germinated seedlings from 'sanguenelli ... | 2011 | 21714779 |
| targeting excessive free radicals with peels and juices of citrus fruits: grapefruit, lemon, lime and orange. | a comparative study between the antioxidant properties of peel (flavedo and albedo) and juice of some commercially grown citrus fruit (rutaceae), grapefruit (citrus paradisi), lemon (citrus limon), lime (citrusxaurantiifolia) and sweet orange (citrus sinensis) was performed. different in vitro assays were applied to the volatile and polar fractions of peels and to crude and polar fraction of juices: 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging capacity, reducing power and inhibition o ... | 2010 | 19770018 |
| comparative itraq proteome and transcriptome analyses of sweet orange infected by candidatus liberibacter asiaticus. | citrus huanglongbing (hlb) has been threatening citrus production worldwide. in this study, a comparative proteomic approach was applied to understand the pathogenic process of hlb in affected sweet orange leaves. using the isobaric tags for relative and absolute quantification (itraq) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'madam vinous' sweet orange plants. of the identified proteins, 20 and 10 were differentially expressed in lea ... | 2011 | 21838733 |
| roles of olfactory cues, visual cues, and mating status in orientation of diaphorina citri kuwayama (hemiptera: psyllidae) to four different host plants. | diaphorina citri kuwayama (hemiptera: psyllidae) is an important worldwide pest of citrus that vectors bacteria (candidatus liberibacter spp.) responsible for huanglongbing (citrus greening disease). we examined the behavioral responses of mated and unmated d. citri of both sexes to odors from host plants in a y-tube olfactometer, with and without visual cues. the host plants tested were 'duncan' grapefruit (citrus paradisi macfayden), sour orange (citrus aurantium l.), navel orange (c. sinensis ... | 2009 | 19791618 |
| metal analysis in citrus sinensis fruit peel and psidium guajava leaf. | the determination of metal traces is very important because they are involved in biological cycles and indicate high toxicity. the objective of the present study is to measure the levels of heavy metals and mineral ions in medicinally important plant species, citrus sinensis and psidium guajava. this study investigates the accumulation of copper (cu), zinc (zn), cadmium (cd), aluminum (al), mercury (hg), arsenic (as), selenium (se) and inorganic minerals like calcium (ca) and magnesium (mg) in c ... | 2011 | 21976824 |
| Comparison of ascorbate metabolism in fruits of two citrus species with obvious difference in ascorbate content in pulp. | Citrus fruit is widely consumed and provides ascorbate for human health. The ascorbate content in pulp is generally higher in orange (Citrus sinensis Osb.) than in Satsuma mandarin (Citrus unshiu Marc.). However, what contributes to such difference is still unknown. In the present study, ascorbate accumulation, expression profiles of genes involved in L-galactose pathway and activity changes of enzymes related with L-ascorbic acid (AA) oxidation and recycling were investigated during fruit devel ... | 2011 | 21925761 |
| effect of genotype and environment on citrus juice carotenoid content. | a selection of orange and mandarin varieties belonging to the same citrus accession and cultivated in mediterranean (corsica), subtropical (new caledonia), and tropical areas (principally tahiti) were studied to assess the effect of genotype and environmental conditions on citrus juice carotenoid content. juices from three sweet orange cultivars, that is, pera, sanguinelli, and valencia ( citrus sinensis (l.) osbeck), and two mandarin species ( citrus deliciosa ten and citrus clementina hort. ex ... | 2009 | 19807162 |
| evaluation of effects of zingiber officinale on salivation in rats. | there are some herbal plants in iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. the aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. the extracts of 7 herbs; zingiber officinale roscoe (zingiberaceae), citrus sinensis (l.) osbeck (rutaceae), artemisia absinthium l. (asteraceae), cichorium intybus l. (asteraceae), pimpinell ... | 2011 | 21874635 |
| in vitro inhibition of vancomycin-susceptible and vancomycin-resistant enterococcus faecium and e. faecalis in the presence of citrus essential oils. | glycopeptide-resistant enterococcus (gre) is an important healthcare-acquired infection (hcai) which costs the healthcare service many millions of pounds worldwide. in this study, lemon (citrus limon), sweet orange (citrus sinensis) or bergamot (citrus bergamia) essential oils (eo) and their vapours, alone and in combination, are tested for their antimicrobial activity against vancomycin-resistant and vancomycin-sensitive strains of e. faecium and e. faecalis. a blend of 1:1 (v/v) orange and ber ... | 2009 | 20095125 |
| ciprofloxacin-sensitive and ciprofloxacin-resistant campylobacter jejuni are equally susceptible to natural orange oil-based antimicrobials. | a total of 10 ciprofloxacin-sensitive (ciprofloxacin minimum inhibitory concentration, mic < 0.5 micro g/ml) and 10 ciprofloxacin-resistant (mic 16 to 32 micro g/ml) presumptive c. jejuni were further characterized and evaluated for their inhibition by natural orange oil fractions. partial species identification was performed by using a hippuricase gene-based polymerase chain reaction (pcr) assay. one of the isolates appeared to be atypical and failed to hydrolyze hippurate. of the ciprofloxacin ... | 2009 | 20183064 |
| cloning, purification and characterization of a 90kda heat shock protein from citrus sinensis (sweet orange). | protein misfolding is stimulated by stress, such as heat, and heat shock proteins (hsps) are the first line of defense against these undesirable situations. plants, which are naturally sessile, are perhaps more exposed to stress factors than some other organisms, and consequently, the role of hsps is crucial to maintain homeostasis. hsp90, because of its key role in infection and other stresses, is targeted in therapies that improve plant production by increasing resistance to both biotic and ab ... | 2012 | 21873074 |
| acaricidal activity against tetranychus urticae and chemical composition of peel essential oils of three citrus species cultivated in ne brazil. | the repellency and fumigant toxicities of the peel essential oils of citrus sinensis var. pêra (lp), c. sinensis var. mimo (lm), and c. aurantium (ll) cultivated in northeast brazil were evaluated against tetranychus urticae. analysis of the oils by gc and gc/ms led to the identification of twenty-eight components, which represented 99.9%, 99.7% and 99.3% of the total constituents of the lp, lm and ll oils, respectively. limonene was the main component found in all three oils. other main compone ... | 2010 | 20420330 |
| efficacy of killer yeasts in the biological control of penicillium digitatum on tarocco orange fruits (citrus sinensis). | killer saccharomyces cerevisiae and wickerhamomyces anomalus yeast strains were tested as biocontrol agents against penicillium digitatum, one the most important causes of postharvest decay in orange fruits. w. anomalus, grown on acidified medium, demonstrated micocinogenic activity against p. digitatum, as indicated by large inhibition halos and hyphal damage resulting from β-glucanase activity. oranges that had been deliberately inoculated with pathogens were protected from decay by w. anomalu ... | 2012 | 22265304 |
| activity of citrus essential oils against escherichia coli o157:h7 and salmonella spp. and effects on beef subprimal cuts under refrigeration. | escherichia coli o157:h7 and salmonella spp. are bacterial pathogens often associated with beef, and cause many cases of foodborne illness each year in the united states. during beef slaughter and processing, these bacteria may spread from the hide or intestines to the carcass. the objective of this research was to investigate the use of naturally occurring compounds citrus essential oils (ceos) extracted from orange peel to reduce or eliminate these pathogens at the chilling stage of processing ... | 2011 | 22417514 |
| transcriptional profiling of canker-resistant transgenic sweet orange (citrus sinensis osbeck) constitutively overexpressing a spermidine synthase gene. | citrus canker disease caused by xanthomonas citri subsp. citri (xcc) is one of the most devastating diseases affecting the citrus industry worldwide. in our previous study, the canker-resistant transgenic sweet orange (citrus sinensis osbeck) plants were produced via constitutively overexpressing a spermidine synthase. to unravel the molecular mechanisms underlying xcc resistance of the transgenic plants, in the present study global transcriptional profiling was compared between untransformed li ... | 2012 | 23509803 |
| chemical characterization of orange juice from trees infected with citrus greening (huanglongbing). | the effects due to candidatus liberibacter infection, commonly called citrus greening or huanglongbing (hlb), on volatile and nonvolatile components of orange juices, oj, were examined using gc-ms and high-performance liquid chromatography (hplc). hlb symptomatic, asymptomatic, and control "hamlin" and "valencia" oranges were harvested from december to may during the 2007 to 2008 harvest season. brix/acid levels in control and asymptomatic juices were similar but symptomatic juices were as much ... | 2010 | 20492226 |
| early events of citrus greening (huanglongbing) disease development at the ultrastructural level. | citrus greening (huanglongbing [hlb]) is one of the most destructive diseases of citrus worldwide. the causal agent of hlb in florida is thought to be 'candidatus liberibacter asiaticus'. understanding of the early events in hlb infection is critical for the development of effective measures to control the disease. in this work, we conducted cytopathological studies by following the development of the disease in citrus trees graft inoculated with 'ca. l. asiaticus'-containing material under gree ... | 2010 | 20701493 |
| effect of ploidy increase on transgene expression: example from citrus diploid cybrid and allotetraploid somatic hybrid expressing the egfp gene. | polyploidization is an important speciation mechanism for all eukaryotes, and it has profound impacts on biodiversity dynamics and ecosystem functioning. green fluorescent protein (gfp) has been used as an effective marker to visually screen somatic hybrids at an early stage in protoplast fusion. we have previously reported that the intensity of gfp fluorescence of regenerated embryoids was also an early indicator of ploidy level. however, little is known concerning the effects of ploidy increas ... | 2010 | 20734092 |
| antimicrobial effect and mode of action of terpeneless cold-pressed valencia orange essential oil on methicillin-resistant staphylococcus aureus. | the objectives of this study were to evaluate the antistaphylococcal effect and elucidate the mechanism of action of orange essential oil against antibiotic-resistant staphylococcus aureus strains. | 2012 | 22372962 |
| a lov protein modulates the physiological attributes of xanthomonas axonopodis pv. citri relevant for host plant colonization. | recent studies have demonstrated that an appropriate light environment is required for the establishment of efficient vegetal resistance responses in several plant-pathogen interactions. the photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. data obtained from bacterial genome sequences revealed the presence of photosensory proteins of the bluf (blue light sensing using fad), lov (light, oxygen, voltage) and phytochrome families with no known functi ... | 2012 | 22675525 |
| the lov protein of xanthomonas citri subsp. citri plays a significant role in the counteraction of plant immune responses during citrus canker. | pathogens interaction with a host plant starts a set of immune responses that result in complex changes in gene expression and plant physiology. light is an important modulator of plant defense response and recent studies have evidenced the novel influence of this environmental stimulus in the virulence of several bacterial pathogens. xanthomonas citri subsp. citri is the bacterium responsible for citrus canker disease, which affects most citrus cultivars. the ability of this bacterium to coloni ... | 2013 | 24260514 |