Publications

TitleAbstractYear
Filter
PMID
Filter
differentiation of some species of neisseriaceae and other bacterial groups by dna-dna hybridization.dna-dna hybridization using total genomic dna probes may represent a way of differentiating between miscellaneous bacterial species. this was studied with type and reference strains of 20 species in moraxella, kingella, and other selected gram-negative groups. both radioactive and biotin labelling were employed. most of the species examined were easily distinguished, such as moraxella (branhamella) catarrhalis, m.(b.) ovis, m. atlantae, m. phenylpyruvica, m. osloensis, neisseria elongata, n. men ...19892730785
[genital infections in adolescence].in 126 patients with relapsing cervicitis or abnormal cytologic findings, aged 13-19 years (12.5% were virgins), the most frequently bacteria were ureaplasma urealyticum (30.2%), mycoplasma hominis, and gardnerella vaginalis (14.3%), and chlamydia trachomatis (9.5%). in patients with abnormal cytologic findings, ureaplasma urealyticum was isolated most frequently--5 times in group iiia after papanicolaou and 4 times in group iiib, followed by mycoplasma hominis 3 times in group iiia and iiib eac ...19892739437
diagnostic procedures in vaginitis.ninety-seven vaginitis patients underwent a standardized direct method of examination. bacterial vaginosis was found in 38.1%, candida albicans in 24.7% and trichomonas vaginalis in 13.4%. low secretional ph values were never met in bacterial vaginosis and t. vaginalis cases. a fishy odor, present in bacterial vaginosis, was also found in almost half of the t. vaginalis cases. endocervical bacteriological cultures are probably insufficient with respect to the detection of c. albicans and gardner ...19892759324
comparison of the in vitro activities of fenticonazole, other imidazoles, metronidazole, and tetracycline against organisms associated with bacterial vaginosis and skin infections.the in vitro antibacterial activity of the antifungal compound fenticonazole was compared with those of clotrimazole, miconazole, tetracycline, and metronidazole against 177 strains of bacterial species associated with either bacterial vaginosis (bv) or skin infections by agar dilution mic determinations. bv-associated bacteroides isolates of the bacteroides melaninogenicus-b. oralis group, gardnerella vaginalis, mobiluncus spp., and anaerobic, gram-positive cocci were highly susceptible to fent ...19892764547
evaluation and management of vaginitis. an update for primary care practitioners.there are three major causes of vaginitis symptoms that primary care practitioners should be able to diagnose and treat expertly. recent investigations have shown that bacterial vaginosis, the most common cause of vaginitis symptoms in patients not seen in sexually transmitted disease clinics, has a multifactorial etiology: gardnerella vaginalis is universally present but is not, per se, the etiologic agent. diagnosis and treatment are based, therefore, on evidence of a disturbed bacterial ecolo ...19892645843
early postpartum endometritis: the role of bacteria, genital mycoplasmas, and chlamydia trachomatis.to characterize the flora of early postpartum endometritis and the clinical features of women with specific organisms, endometrial cultures for facultative and anaerobic bacteria, genital mycoplasmas, and chlamydia trachomatis were taken with a triple-lumen sampling device. more than one organism was recovered from 80% of the women. over 60% of the women had gardnerella vaginalis and/or anaerobes associated with bacterial vaginosis isolated from the endometrium; these women were more likely to h ...19892783262
vaginal infection and preterm labour.to study the vaginal flora of women in preterm labour (ptl) and determine whether the presence of specific vaginal microflora is significantly associated with onset of ptl.19912059587
quality control standards for the proline aminopeptidase assay used to detect bacterial vaginosis.we describe the preparation and usage of positive and negative quality control standards for the proline aminopeptidase assay of vaginal secretions as an indicator of bacterial vaginosis.19892784630
bacteriological state of the urine in symptom-free adult males.mid-stream urine specimens from 120 symptom-free males aged between 15 and 60 years were examined for cells and bacteria. only 3 specimens showed pyuria, all of which yielded fastidious organisms. no specimen yielded a pure growth of an aerobe. fastidious organisms in pure or mixed growth were isolated from 26% of the specimens, none in a count greater than 10(8)/l and the majority in counts less than 10(7)/l. it was concluded that pure growths of aerobes in any count in patients with symptoms a ...19902132301
the cytologist and bacterioses of the vaginal-ectocervical area. clues, commas and confusion.to evaluate the role of the cytologist in the diagnosis of bacterial vaginosis, the predominant bacterial patterns seen in 157 papanicolaou-stained cervical smears were assessed and classified as large bacillus, anaerobic or scanty. a large bacillus pattern was found in 73 smears (46%), an anaerobic pattern in 77 (49%) and scanty bacteria in 7 (5%). comma-shaped (curved) bacilli were found in 34 smears. the prevalence of clue cells in smears with anaerobic patterns was 73%. gardnerella vaginalis ...19892786305
a follow-up study of methods of contraception, sexual activity, and rates of trichomoniasis, candidiasis, and bacterial vaginosis.a randomized, clinical trial was conducted to evaluate the spermicidal agent nonoxynol 9 as prophylaxis for sexually transmitted diseases. eight hundred eighteen women using birth control who attended a sexually transmitted disease clinic were evaluated monthly for trichomoniasis, candidiasis, and bacterial vaginosis for 6 months. women using the active spermicide experienced a somewhat lower incidence rate of trichomoniasis (relative rate 0.83; 95% confidence interval 0.61 to 1.12) and bacteria ...19902167008
bacterial vaginosis: diagnostic and pathogenetic findings during topical clindamycin therapy.we examined subjective and objective correlates among 67 women with symptomatic bacterial vaginosis before and after treatment with intravaginal clindamycin or placebo. we found no preponderance of any sexual practices among these patients. nine patients (13.4%) had had hysterectomy. whereas odor and discharge were the most common symptoms, 30 patients (44.8%) also complained of vulvovaginal irritation. symptoms correlated poorly with objective therapeutic outcome. on examination the diagnosis w ...19902201189
vaginitis emphysematosa. a report of four cases.it has been hypothesized that vaginitis emphysematosa is a manifestation of trichomonal or gardnerella infection. in support of this etiologic concept, four cases of the disorder are described showing the apparent curative effect of treating the associated infection. the linkage of these and other reported cases with conditions of impaired immunity suggests that immunologic factors are involved in the pathogenesis.19902246767
[management of a case of non specific vaginitis]. 19902260808
chemotaxis inhibition by gardnerella vaginalis and succinate producing vaginal anaerobes: composition of vaginal discharge associated with g vaginalis.the influence of six succinate producing vaginal anaerobes and gardnerella vaginalis on the chemotactic activity of granulocytes was studied by the under agarose method. g vaginalis, mobiluncus species, and three gram negative anaerobes elicited hardly any response, but peptostreptococcus productus showed clear positive chemotaxis, as did the escherichia coli strain used as a control. inhibition of the chemotactic response of white blood cells was found with all strains, but the high succinate p ...19892787775
[intravaginal bacterial flora in patients with uterine cervical cancer, with special reference to gardnerella vaginalis].in a total of 18 subjects, comprising 16 cases of uterine cervical cancer (4 cases, stage ib; 1 case, stage iia; 11 cases, stage iib) and 2 cases of cervical dysplasia, the intravaginal bacterial floras, including gardnerella vaginalis (g. vaginalis) and mobiluncus spp., were investigated. in uterine cervical cancer, there were mixed bacterial flora of aerobes and anaerobes but what is of note is that g. vaginalis was detected in 7 out of 16 cases of uterine cervical cancer and in 1 out of 2 cas ...19902273311
longitudinal study of the biotypes of gardnerella vaginalis.gardnerella vaginalis is the predominant vaginal microorganism in women with bacterial vaginosis. however, this organism is also frequently isolated from women without signs or symptoms of vaginitis. earlier studies have not revealed whether certain biotypes of g. vaginalis are more often associated with bacterial vaginosis or are more common in women who acquire bacterial vaginosis. we used a typing scheme based on tests for beta-galactosidase, hippurate hydrolysis, and lipase, using oleate as ...19902280007
vaginitis and pregnancy.vaginitis in pregnant women can affect the outcome of the pregnancy through preterm labor, premature rupture of the membranes, chorioamnionitis, neonatal infection and postpartum endometritis. women can be screened for group b streptococci, and infection in the newborn can be prevented by the institution of antibiotic therapy in labor. screening pregnant women for bacterial vaginitis can reduce the risk of premature rupture of the membranes, premature labor and neonatal infection.19892795573
bacterial vaginosis as a risk factor for post-cesarean endometritis.bacterial species associated with bacterial vaginosis have been isolated more frequently from endometrial cultures of patients with postpartum endometritis than expected from the prevalence of bacterial vaginosis among pregnant women. to further assess the association between bacterial vaginosis and postpartum endometritis, vaginal gram smears were obtained from women admitted for delivery. vaginal smears of women delivered by cesarean were scored as normal or as indicating bacterial vaginosis. ...19902296423
sexually transmitted pathogens in pregnant women in a rural south african community.one hundred and ninety three consecutive pregnant women attending peripheral antenatal clinics attached to ngwelezana hospital, empangeni, kwa-zulu, were examined for evidence of sexually transmitted pathogens. the following incidences were found: trichomonas vaginalis 49.2% (95), candida spp 38.3% (74), chlamydia trachomatis 11.4% (22), gardnerella vaginalis 6.2% (12), neisseria gonorrhoeae 5.7% (11), positive syphilis serology results 11.9% (23), hepatitis b surface antigen 4.1% (eight). no wo ...19892807289
importance of gardnerella vaginalis as an aetiological agent in bacterial vaginosis. 19892807292
comparative in-vitro activity of meropenem on clinical isolates from the united kingdom.mics of meropenem were determined for a wide range of common bacteria of clinical importance. for enterobacteriaceae, aeromonas spp., haemophilus influenzae, branhamella catarrhalis, neisseria gonorrhoeae, gardnerella vaginalis, campylobacter coli/jejuni, beta-haemolytic streptococci and anaerobes other than clostridium difficile, mics were almost always within the range 0.002-0.5 mg/l. the activity of meropenem for these organisms was always greater than that of imipenem and piperacillin, and w ...19892808214
gardnerella vaginalis as a urinary pathogen. 19892809241
cervical mosaic and an integrated pathophysiological approach to early cervical neoplasia.the increasing concern for the early detection and treatment of cervical intraepithelial neoplasia (cin) set up the basis for this study, which also considers some epidemiological factors which seem to be related to the genesis of an abnormal uterine cervical colposcopic pattern, the mosaic pattern. we have observed that women with a mosaic pattern show an increasing frequency of premalignant and/or malignant histologic changes up to 40 years of age which then falls abruptly. sterility and hirsu ...19872824702
[diagnosis of flat condylomas of the uterine cervix: problems with associated infections and differential diagnosis]. 19872829756
controlled evaluation of trypticase soy broth with and without gelatin and yeast extract in the detection of bacteremia and fungemia.the addition of gelatin to blood culture media has been suggested to prevent the inhibition of neisseria meningitidis, neisseria gonorrhoeae, gardnerella vaginalis, and peptostreptococcus anaerobius that is caused by sodium polyanetholsulfonate. to determine the effect of such supplementation on the overall yield of microorganisms, we compared the yield and speed of detection of clinically important microorganisms from 5422 paired 10-ml samples of blood cultured in trypticase soy broth (tsb) con ...19872831009
[in vitro effect of nonoxynol-9 on herpes virus 1 and 2 and on other sexually transmitted micro-organisms]. 19882854228
streptococci as urinary pathogens.in a 2-month prospective study of streptococci isolated from urine specimens in the laboratory, 242 strains of catalase-negative gram-positive cocci or coccobacilli were isolated in substantial numbers from 11,725 specimens. these comprised 10% of the important isolates. species identification of all isolates was undertaken. 74 (30%) of the isolates were of species other than streptococcus faecalis and s agalactiae. 79 (33%) were not detected on cysteine-lactose-electrolyte-deficient agar after ...19862875237
pili on gardnerella vaginalis studied by electronmicroscopy.fourteen recently isolated strains and two laboratory strains of gardnerella vaginalis were examined by electronmicroscopy for the presence of pili. all strains isolated recently from both men and women were heavily pilated. in contrast only a few pili were seen on organisms of the two laboratory strains, with many of the organisms having no pili. the importance of multiple subculture in this loss was supported by the observation that the degree of pilation of one freshly isolated strain decreas ...19872884322
effect of low-dose oral contraceptives on natural killer cell activity.several reports indicate an association between oral contraceptives and increased infection rates. one mechanism that could explain this increased infection rate is a decrease in immune function. a study comparing t cell subsets showed no differences in numbers between oral contraceptive users and controls. in this study, natural killer cell activity was compared in women before and 3 and 6 months after oral contraceptive use. there was a statistically significant decrease in nk cell activity af ...19892910646
prevalence of hydrogen peroxide-producing lactobacillus species in normal women and women with bacterial vaginosis.a predominance of lactobacillus species in the vaginal flora is considered normal. in women with bacterial vaginosis, the prevalence and concentrations of intravaginal gardnerella vaginalis and anaerobes are increased, whereas the prevalence of intravaginal lactobacillus species is decreased. because some lactobacilli are known to produce hydrogen peroxide (h2o2), which can be toxic to organisms that produce little or no h2o2-scavenging enzymes (e.g., catalase), we postulated that an absence of ...19892915019
gardnerella vaginalis in posthysterectomy infection.eighty-six women were admitted for abdominal hysterectomy. preoperatively, 43 women were culture-positive for gardnerella vaginalis from the cervical os, and 43 women were culture-negative. postoperatively, 18 patients developed inflammation; among those 14 patients from the culture-positive group were infected, whereas only 4 patients from the culture-negative group developed inflammation. the difference between occurrence of g. vaginalis and the absence of the bacteria in patients with postope ...19902311819
the comparative in-vitro activity of pefloxacin.the in-vitro antibacterial activities of pefloxacin, other 4-quinolones and representative beta-lactams and aminoglycosides were assessed by determination of minimum inhibitory concentrations (mics). pefloxacin (mics mostly 0.03-2 mg/l) was highly active against enterobacteriaceae. gentamicin had slightly lower activity, and ceftazidime and norfloxacin similar activities to pefloxacin whereas ciprofloxacin was more active. pefloxacin (mics 0.03-2 mg/l) was active against acinetobacter but again ...19862940213
salpingitis; aspects of diagnosis and etiology: a 4-year study from a swedish capital hospital.359 patients underwent laparoscopy to verify the diagnosis of salpingitis. pelvic inflammatory disease (pid) was found in 187 (52%) cases. laparoscopy revealed normal conditions in 136 (37%) cases. other diseases were diagnosed in 36 (10%) cases. bacteriological cultures from the fimbrial lumen were positive in 24% of the pid cases. chlamydia trachomatis (ct) was detected in 12%, bacteroides species (bs) in 5%, actinomyces israelii (ai) in 3%, gardnerella vaginalis (gv) in 2%, neisseria gonorrho ...19872951285
[culture and identification of gardnerella vaginalis]. 19882969298
trospectomycin, a novel spectinomycin analogue: antibacterial activity and preliminary human pharmacokinetics.trospectomycin (tsp; u-63366f) is a novel spectinomycin (sp) analogue with broad-spectrum antibacterial activity. the in vitro activity of the analogue was compared to that of sp against approximately 400 bacterial isolates. the in vivo activity of the compound was assessed using experimental infection models for both gram-positive and gram-negative facultative bacteria. the preliminary human pharmacokinetics of tsp were evaluated following single-dose i.v. or i.m. administration. tsp was more a ...19882975212
the association of sexually transmitted diseases with cervical intraepithelial neoplasia: a case-control study.thirty-three women with histologically confirmed cervical intraepithelial neoplasia (grades i to iii, with one case of microinvasive carcinoma) and 54 women without evidence of the disease were prospectively studied to determine the relationship of genital infection to cervical neoplasia. demographic and sexual data for patients and control subjects were collected, with standardized clinical and colposcopic evaluation by means of predefined diagnostic categories. cultures from the cervix were ex ...19852982264
bacterial vaginosis and trichomoniasis vaginitis are risk factors for cuff cellulitis after abdominal hysterectomy.to assess the relationship between either bacterial vaginosis or trichomoniasis vaginitis and posthysterectomy infection, preoperative evaluation of the vaginal secretions was performed in 161 women undergoing abdominal hysterectomy. thirty-two patients (19.9%) and 27 patients (16.8%), respectively, met the diagnostic criteria for bacterial vaginosis and trichomoniasis vaginitis. patients with either bacterial vaginosis or trichomoniasis vaginitis were more likely than control subjects to have c ...19902403128
effect of sodium polyanetholesulfonate and gelatin on the recovery of gardnerella vaginalis from blood culture media.sodium polyanetholesulfonate (sps) is used as a routine supplement to blood culture media to enhance recovery of microorganisms, but it inhibits the growth of peptostreptococcus anaerobius, neisseria meningitidis, neisseria gonorrhoeae, and streptobacillus moniliformis. comparative clinical blood culture studies at the university of colorado hospital suggested that sps also inhibits the growth of gardnerella vaginalis. we inoculated 16 blood culture isolates of g. vaginalis into 11 blood culture ...19852987298
sodium polyanethol sulphonate discs to identify gardnerella vaginalis. 19852991122
sodium polyanetholesulfonate in the identification of gardnerella vaginalis. 19852993355
preterm labor associated with subclinical amniotic fluid infection and with bacterial vaginosis.maternal genital infection, particularly subclinical amniotic fluid infection, may cause preterm labor and a premature delivery. the prevalence of subclinical amniotic fluid infection was studied in 54 consecutive afebrile women in preterm labor with singleton gestations and intact fetal membranes. microorganisms were recovered from the amniotic fluid by transabdominal amniocentesis in 13 (24%) of 54 patients. bacteria or candida albicans were recovered from six (11%), and genital mycoplasmas fr ...19863003634
contribution of gardnerella vaginalis to vaginitis in a general practice.in a study of 154 adult women who presented to their general practitioner with vaginal symptoms 30 (20%) had gardnerella vaginalis on its own and 51 (33%) had g vaginalis in combination with anaerobes or known pathogens. thirty one (20%) patients were culture negative. those who were culture negative had fewer symptoms and signs of vaginitis than those with g vaginalis alone or g vaginalis plus anaerobes. those with known pathogens had more symptoms and signs than those with g vaginalis alone or ...19863013357
sulbactam/ampicillin versus metronidazole/gentamicin in the treatment of severe pelvic infections.the clinical efficacy and safety of sulbactam/ampicillin versus metronidazole/gentamicin were compared in 39 patients with severe pelvic infections. 30 patients had severe acute pelvic inflammatory disease with peritonitis, 3 tubo-ovarian abscesses, 4 endomyometritis, and 2 posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures from the sites of infection yielded 259 micro-organisms from 38 patients; an average of 6.8 bacteria per infection (3.9 anaerobes and 2.9 aerobes). the most f ...19863013566
ampicillin/sulbactam versus metronidazole-gentamicin in the treatment of soft tissue pelvic infections.the clinical efficacy and safety of ampicillin/sulbactam versus metronidazole-gentamicin were evaluated in a comparative, randomized, prospective study. forty-four patients were enrolled: 22 received the ampicillin/sulbactam regimen, and 22 received the metronidazole-gentamicin combination. there were 33 cases of severe acute pelvic inflammatory disease, two tuboovarian abscesses, five cases of endomyometritis, and two cases of posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures f ...19873030109
comparison of methods for diagnosing bacterial vaginosis among pregnant women.the diagnosis of bacterial vaginosis is usually based on clinical criteria including homogeneous vaginal discharge, an elevated vaginal ph, the presence of clue cells, and an amine odor. we have evaluated the vaginal flora and clinical signs for 593 pregnant women. gardnerella vaginalis, bacteroides spp., and mycoplasma hominis were isolated more frequently among women with clinical signs than among those without clinical signs of bacterial vaginosis in multivariable analyses that controlled for ...19892473992
bacterial vaginosis.bacterial vaginosis (nonspecific vaginitis) is a polymicrobial, superficial vaginal infection caused by an increase in anaerobic organisms and a concomitant decrease in lactobacilli. gardnerella vaginalis, once thought to be the sole etiologic agent, is probably one of several endogenous members of the vaginal flora that overgrow in women with bacterial vaginosis. whether the growth of anaerobes or a primary decrease in lactobacilli is the initial pathogenic event remains unclear. epidemiologica ...19883042906
a 24 hour plastic envelope method for isolating and identifying gardnerella vaginalis (pem-gva)a new plastic envelope culture test that is selective for gardnerella vaginalis was compared with a conventional method. vaginal specimens from 92 women were cultured. results from both methods were compared with the results of pelvic examinations and clinic screening tests used to diagnose bacterial vaginosis (bv). g vaginalis was isolated more often in the envelope than by the conventional method from patients with bv and those without, though the difference was not significant. isolation and ...19883044977
the cervical cap.the us food and drug administration has approved marketing of the prentif cavity-rim cervical cap. this contraceptive device is being distributed in the us and canada by cervical cap ltd, los gatos, california. the prentif cap is available in 4 sizes: 22, 25, 28, and 31 mm inside diameter, with a length of 1 1/4-1 1/2 inches. in a multicenter trial involving 522 diaphragm users and 581 cap users followed for 2 years, the cap was 82.6% effective and the diaphragm was 83.3% effective in preven ...19883050407
[recurrent vaginitis].vaginitis is one of the most frequent infections of the female genital system and, in the united states, represents the main reason for gynecological consultation. candida albicans, trichomonas vaginalis and gardnerella vaginalis are responsible for 90% of cases of vaginal phlogosis. it has been calculated that a percentage varying between 5% and 40% of the female population will suffer, at least once in their lifetime, from an episode of vaginal candidosis. the re-appearance of symptoms after s ...19892484480
bacterial vaginosis: a comprehensive review.in summary, bacterial vaginosis infections are very common, easy to diagnose, and usually quite simple to treat. rarely are symptoms dramatic, and often the woman is unaware of the presence of this infection. partners should be treated, and thorough health education should be provided to both patient and partner. further research is needed to develop safer, equally effective treatments for this infection. currently metronidazole is the drug of choice, except in pregnant women. possible serious p ...19883057469
preterm labor: the role of amnionitis.this prospective study represents an attempt to find a link between subclinical intra-amniotic infection and preterm singleton labor in 27 asymptomatic patients with intact membranes. liquor amnii was obtained by transabdominal amniocentesis and cultured for anaerobic and aerobic bacteria and mycoplasmas. none of these cultures was positive. the value of gas-liquid chromatographic determination of volatile and non-volatile acids in liquor amnii could not be determined because amniotic infections ...19883062950
[in vitro activity of 5 new quinolones against gardnerella vaginalis].the minimal inhibitory concentrations (mics) of five new quinolones were determined by agar dilution, for 50 clinical isolates of gardnerella vaginalis. they are compared with those of metronidazole, ampicillin, erythromycin and tetracycline which are widely used for the treatment of lower genital tract infections in women. the mics of rosoxacin and pefloxacin are high, but those of ofloxacin (mic 50, 2 mg/l), fleroxacin (mic 50, 2 mg/l) and ciprofloxacin (mic 50, 1 mg/l) are lower. they can exp ...19892550875
prevalence of nonspecific vaginitis and other cervicovaginal infections during the third trimester of pregnancy.nonspecific vaginitis (nsv) is a clinical syndrome characterized by a decreased concentration of normal acidophilic döderlein flora in the vagina and increased concentration of nonacidophilic microorganisms, such as gardnerella vaginalis, anaerobic bacteria, and mycoplasmas. since several nsv-associated organisms are associated with complications of pregnancy, we studied the prevalence of nsv among 104 consecutive women during the third trimester of pregnancy. overall, 14 women (14%) had nsv by ...19863082020
sexually transmissible infectious agents in sexually active and virginal asymptomatic adolescent girls.sixty-eight sexually active and 52 virginal adolescent girls were evaluated for six sexually transmissible infectious agents: gardnerella vaginalis, ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis, trichomonas vaginalis, and neisseria gonorrhoeae. there were significant differences between sexually active and virginal girls with respect to the prevalence of isolation of u urealyticum (75% v 33%, p less than .005), m hominis (27% v 10%, p less than .05), and c trachomatis (19% v ...19863083395
a modified scheme for biotyping gardnerella vaginalis.a scheme is proposed for biotyping gardnerella vaginalis, based on detection of hippurate hydrolysis, beta-galactosidase (onpg) and lipase, and fermentation of arabinose, galactose and xylose. seventeen biotypes were found among 197 strains from asymptomatic women and patients with bacterial vaginosis (non-specific vaginitis). the distribution of biotypes was similar in both populations but some biotypes were found more frequently in patients. the proposed scheme is compared with those previousl ...19863088281
microbiology of vaginal discharge in general practice.three groups of women were examined by culture for gardnerella vaginalis and candida. group i consisted of 427 women, who complained spontaneously of vaginal discharge, group ii of 311 women who did not complain of vaginal discharge until questioned prior to gynaecological examination, and group iii of 100 women who denied vaginal discharge. groups i and ii also had cultures made for trichomonas vaginalis and neisseria gonorrhoica. in group i with spontaneous complaints the one-year prevalence r ...19863088694
an unusual case of gardnerella vaginalis septicaemia. 19863089498
in vitro activity of the two new 4-quinolones a56619 and a56620 against neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum and gardnerella vaginalis.the in vitro activity of tetracycline, ciprofloxacin and two recently developed 1-aryl-fluoroquinolones, a56610 and a56620, was tested against 65 beta-lactamase-negative and 35 beta-lactamase-positive neisseria gonorrhoeae strains, 12 chlamydia trachomatis, 50 mycoplasma hominis, 28 ureaplasma urealyticum and 50 gardnerella vaginalis strains. in the case of chlamydia trachomatis and mycoplasma hominis both the mic and the mbc were determined. the mic90 of ciprofloxacin for neisseria gonorrhoeae ...19863096726
in vitro activity of difloxacin hydrochloride (a-56619), a-56620, and cefixime (cl 284,635; fk 027) against selected genital pathogens.management of sexually transmitted diseases is facilitated by having antimicrobial agents with activity against all of the major genital pathogens. newer quinolones show promise of being active against neisseria gonorrhoeae and chlamydia trachomatis. two quinolones, difloxacin (a-56619) and a-56620, and an oral cephalosporin, cefixime (cl 284,635; fk 027), were evaluated in vitro. all three were highly active against 400 isolates of n. gonorrhoeae, including penicillinase-producing n. gonorrhoea ...19863098163
high-level tetracycline resistance in neisseria gonorrhoeae is result of acquisition of streptococcal tetm determinant.recently, strains of neisseria gonorrhoeae have been isolated which are highly resistant to tetracycline (mics of 16 to 64 micrograms/ml). this resistance was due to the acquisition of the resistance determinant tetm, a transposon-borne determinant initially found in the genus streptococcus and more recently in mycoplasma hominis, ureaplasma urealyticum, and gardnerella vaginalis. in n. gonorrhoeae, the tetm determinant was located on a 25.2-megadalton plasmid. this plasmid arose from the insert ...19863099640
in vitro activity of a-56619 (difloxacin), a-56620, and other new quinolone antimicrobial agents against genital pathogens.the in vitro activities of two new carboxyquinolones, a-56619 (difloxacin) and a-56620, were compared with those of ciprofloxacin, norfloxacin, and ofloxacin against genital tract pathogens. all the quinolones were highly active against neisseria gonorrhoeae. a-56619 had the lowest mics against chlamydia trachomatis (mic range, 0.125 to 0.25 micrograms/ml) and haemophilus ducreyi (mic for 90% of isolates tested, 0.1 micrograms/ml).19863101590
the midcycle cervical microbial flora as studied by the weighed-swab method, and its possible correlation with results of sperm cervical mucus penetration tests.infertile couples undergoing routine investigation for infertility were randomly selected for the study. a quantitative method using weighed swabs was found satisfactory for the study of microbial flora in the midcycle cervical mucus. in 6 of the 20 women, the midcycle cervical mucus was hostile in the sperm cervical mucus penetration tests. the total bacterial counts and the number of species (average, 2.5) isolated from the hostile mucus were significantly greater than those from the receptive ...19873109958
prevalence of lower genital tract infections in pregnancy.a descriptive profile of 106 pregnant women with various forms of cervicitis and vaginitis is provided. fifty women attending individual physicians' private offices are compared with 56 clinic patients: 34 attending a university prenatal teaching clinic and 22 attending a pregnancy termination unit. univariate analysis showed that single women were significantly more likely to be infected by mycoplasmas, yeasts, trichomonads, and gardnerella vaginalis than were married women. teenagers were more ...19883128883
etiology of nongonococcal urethritis. evidence for chlamydia trachomatis and ureaplasma urealyticum.chlamydia trachomatis, ureaplasma urealyticum (t-mycoplasma), and hemophilus vaginalis have previously been considered possible etiological agents in nongonococcal urethritis (ngu). in this study, current c. trachomatis infection was confirmed by culture and (or) micro-immunofluorescence serology in 26 of 69 men experiencing afirst episode of ngu, and 1 of 39 with no urethritis. serum igm immunofluorescent antibody to chlamydia was demonstrated in 16 of 20 men with chlamydia culture positive ngu ...1977300742
evaluation of a fluorescent monoclonal antibody reagent for identification of cultured neisseria gonorrhoeae.we evaluated a new fluorescent monoclonal antibody reagent for confirmation of identity of neisseria gonorrhoeae. the reagent correctly identified all 161 fresh clinical isolates of n. gonorrhoeae, which included 11 penicillinase producing strains (ppng). the reagent also correctly identified 21 stored ppng strains. no cross reactions were seen with 58 fresh clinical isolates of n. meningitidis, 12 stored strains of n. lactamica, or with strains of gardnerella vaginalis, lactobacilli, candida sp ...19883133479
prevalence of gardnerella vaginalis. 19883134106
lower genital tract infections in women: comparison of clinical and epidemiologic findings with microbiology.we screened 392 women attending the laboratory of microbiology of toulouse purpan hospital for symptoms of lower genital tract infection for six microorganisms. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, candida albicans, mycoplasma hominis, and gardnerella vaginalis were 1%, 7.7%, 29.8%, 23.8%, and 37.5%, respectively. trichomonas vaginalis was detected by direct examination in specimens from 3.1% of patients. another goal of this study was to determine the relative pre ...19883135608
neonatal meningitis due to gardnerella vaginalis.a case of meningitis due to gardnerella vaginalis occurred in a five-day-old newborn who had clinical signs of fever, polypnea and a grey complexion. after treatment with ampicillin, cefotaxime and netilmicin, the patient's condition improved, and no sequelae were observed. the bacterium isolated from a pure culture of the cerebrospinal fluid was identified by biochemical characteristics to be gardnerella vaginalis, but it was not possible to define the source and mode of contamination.19883137044
gardnerella vaginalis is associated with other sexually transmittable microorganisms in the male urethra.in a prospective study, urethral swabs were taken from 544 men presented to an std clinic, 118 with and 426 without urethritis, and examined by microscope and/or culture for g. vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum, mycoplasma hominis, candida species and trichomonas vaginalis. g. vaginalis was isolated from 4.2% of the males with urethritis and from 6.3% of those without urethritis. using loglinear analysis, the following associations were significant ( ...19883140532
in vitro activity of azithromycin and erythromycin against organisms associated with bacterial vaginosis and chancroid.the in vitro activity of the new macrolide azithromycin and erythromycin against haemophilus ducreyi, gardnerella vaginalis, bacteroides spp. and mobiluncus spp. was compared. gardnerella vaginalis, mobiluncus spp. and haemophilus ducreyi were highly susceptible to both agents but azithromycin was more effective overall against all three groups of organisms. the activity of both antibiotics against bacteroides spp. varied greatly, but azithromycin was again more active. collectively, 62% of all ...19883141171
microbiological profile of the cervix in 1,000 sexually active women.one thousand consecutive women who attended the richmond family planning association clinic and who were to undergo a vaginal examination were asked to participate in a large prospective microbiological study. participants were questioned about their sexual activity during the previous 12 months and any apparent signs of sexually transmitted disease. on examination the cervix was inspected for evidence of inflammation, ectopy or discharge and cervical swabs were taken microbiological assessment. ...19883148302
electron microscopy of adhesive interactions between gardnerella vaginalis and vaginal epithelial cells, mccoy cells and human red blood cells.exfoliated vaginal epithelial cells with attached bacteria, termed 'clue cells', which were procured from a patient with non-specific vaginitis, were stained with ruthenium red and examined by transmission electron microscopy. the attached bacteria appeared to adhere by means of an outer fibrillar coat. an epithelial tissue culture cell line (mccoy) and human red blood cells to which strains of gardnerella vaginalis attached were similarly examined. the adherence of g. vaginalis to the epithelia ...19892576032
the in-vitro activities of enoxacin and ofloxacin compared with that of ciprofloxacin.the in-vitro activities of enoxacin and ofloxacin were compared with that of the other new 4-quinolone, ciprofloxacin. all three compounds were highly active against enterobacteriaceae, haemophilus influenzae and neisseria gonorrhoeae (mics mostly less than 1 mg/l). the other gram-negative aerobes tested were in general less susceptible, though for acinetobacter and pseudomonas species (including aeruginosa) mics seldom exceeded 8 mg/l. ofloxacin and ciprofloxacin were more active against gardne ...19853159712
the in-vitro activity of pd127,391, a new quinolone.mics of pd127,391 a new 4-quinolone, and of ci934 and ciprofloxacin, two previously reported 4-quinolones, were determined for common clinical bacterial isolates by an agar-dilution method. pd127,391 was the most active drug against enterobacteriaceae and acinetobacter spp (mics less than 0.12 mg/l) and as active as ciprofloxacin against aeromonas spp. (mics less than 0.008 mg/l) and pseudomonas aeruginosa (mics less than 1 mg/l). it was more active than ciprofloxacin against pseudomonas spp. in ...19883182416
a randomized study of antibiotic therapy in idiopathic preterm labor.a randomized study was undertaken to test the effects of antibiotics as an adjunct to established methods of tocolysis. one hundred fifty patients with cervical dilation of 1 cm or more and no clinically identifiable cause for preterm labor qualified for the study. fifty-three (35%) received 500 mg ampicillin orally every 6 hours, 50 (33%) received 500 mg erythromycin orally every 6 hours, and 47 served as controls. antibiotics were prescribed for 10 days, and the treatment was not altered by th ...19883186088
prevalence of non-specific vaginitis and correlation with isolation of gardnerella vaginalis in italian outpatients.among all subjects attending a center for prevention of female genital tract cancer gardnerella vaginalis was isolated from 57 out of 171 women affected by non-specific vaginitis (nsv), from 15 of 703 non-nsv vaginitis patients and from 10 of 338 healthy adults. postmenopausal women showed an increased rate of asymptomatic carriage of g. vaginalis. the data suggest a relatively weak association between clinical diagnosis of nsv and isolation of g. vaginalis.19892606183
heamophilus vaginalis (corynebacterium vaginal): method for isolation and rapid biochemical identification. 1977300733
prevalence of common infective agents of vaginitis.a total of 100 women with vaginitis and 50 age matched healthy women were studied for common infective agents of vaginitis. the vaginal secretions collected from these women were examined for trichomonas vaginalis, g. vaginalis, and candida species. the prevalence of infective agents in the patients was t. vaginalis 11 per cent. candida species 30 per cent and gardnerella vaginalis 31 per cent. in contrast, in the control group the prevalence was candida species 14 per cent and g. vaginalis 22 p ...19892614050
[vaginitis caused by gardnerella vaginalis in children and adolescents]. 19883258756
bacterial vaginosis: etiology, association with preterm labor, diagnosis, and management.bacterial vaginosis is a polymicrobial condition (anaerobes, gardnerella vaginalis) that is associated with symptomatic vaginal discharge. bacterial vaginosis can be reliably diagnosed through clinical indicators such as clue cells on wet preparation of vaginal discharge, an increased ph of vaginal discharge, a fishy, amine odor emitted when a sample of vaginal discharge is placed in potassium hydroxide, and cultures that isolate g. vaginalis. the vaginal discharge is often heavy and foul smelli ...19892670411
the clinical significance of the genital microbiologic flora at vacuum aspiration following miscarriage.85 women undergoing vacuum aspiration following miscarriage were studied. at the time of vacuum aspiration, immunofluorescent stainings for detection of c. trachomatis proved positive in 5.9% of the patients. group b streptococci were isolated in 7.5%, coliforms in 3.5%, and gardnerella vaginalis in 51% of the patients. the overall frequency of postabortal pelvic inflammatory disease (pid) was 3.5%, whereas 40% of chlamydia-positive women contracted postabortal pid (p less than 0.05). no signifi ...19892686341
[gardnerella vaginalis: role of a microorganism emerging as a vaginopathic agent and its placement in the vaginal microbial ecosystem].in this review the role of an emergent pathogen, gardnerella vaginalis (g. vaginalis), as aetiologic agent of the so called "non specific vaginitis" is systematically evaluated. the microorganism is described in its morphological, biochemical and virulence aspects in the framework of the complex vaginal ecosystem. the diagnostic criteria of the disease as well as a possible association of g. vaginalis with other vaginal bacteria, especially anaerobes, in the aetiology of the disease, are discuss ...19892686505
prevalence of gardnerella vaginalis in the urinary tract.midstream urine samples from 106 patients presenting to the casualty department of the royal melbourne hospital with frequency or dysuria were cultured for gardnerella vaginalis and conventional uropathogens. urine samples collected via an open-end catheter from 70 healthy pregnant women were examined similarly. midstream urine and other samples, including the seminal fluids and swabs of the mouths, throats, rectums, and vaginas of 33 healthy subjects, were cultured for g. vaginalis. another 15 ...19883260242
a new look at the diagnosis of infection of the urinary tract and its adjacent structures.the criterion enunciated by kass for interpreting the quantitative examination of urine is critically reappraised. the role of organisms other than those of the aerobic bowel flora, especially fastidious organisms, in urinary tract infections is discussed in detail. clinical microbiologists are urged to pay more attention to the bacteriological examination of urine and to play a greater part in the diagnosis and treatment of infections of the urinary tract and its adjacent structures.19892689521
evidence relating bacterial vaginosis to intraamniotic infection.we performed a two-part study to determine relationships of bacterial vaginosis and intraamniotic infection. in the first part of the study, we determined whether bacterial vaginosis organisms (gardnerella vaginalis, mycoplasma hominis, and anaerobes) were associated with each other in the amniotic fluid of 408 cases of intraamniotic infection. in the second part, we determined the association of bacterial vaginosis itself with intraamniotic infection in 125 cases at high risk for intraamniotic ...19892782365
[gardnerella vaginalis in relation to the clinical syndrome of bacterial vaginosis]. 19883262432
gardnerella vaginalis in infections of the urinary tract.during a period of 6 months, urine from 3576 patients was cultured for gardnerella vaginalis. specimens from 32 patients yielded this species. eleven (0.3%) of the isolates were judged to be of clinical importance. seven were from women and four from men. two of these patients, both women, suffered from spontaneous cystitis. hence g. vaginalis is very unusual as a primary pathogen in the urinary tract. more often it may cause infections related to urological abnormalities or as a complication of ...19892783715
metronidazole-associated pancreatitis. 19883263823
[ultrastructural analysis of the interrelation of the causative agents of urogenital infections with the cells of the macro-organism in syphilis patients]. 19883264100
gardnerella vaginalis: an unusual case of pyogenic liver abscess.we present a case of pyogenic liver abscess caused by gardnerella vaginalis, a previously unreported suppurative complication of infection with this organism. the development of the pylephlebitis originating from endometritis and the possible synergistic interaction of an anaerobic and aerobic organism is reviewed. g. vaginalis has been infrequently isolated in the laboratory; perhaps with refined culturing techniques and recognition of the pathogenic potential of this organism, further cases of ...19883264109
[gardnerella vaginalis in relation to the clinical syndrome of bacterial vaginosis]. 19883264219
neonatal gardnerella vaginalis infection. 19883264904
meningismus as main symptom in toxic shock syndrome.a case of toxic shock syndrome (tss) with meningismus as main symptom is presented. the case illustrates that, in cases of rigidity of the neck and back, not directly explainable, tss should be considered as a possible diagnosis. toxic syndrome (tss) is a severe, acute infectious disease with great variations in symptomatology (1,2). its lethality is approximately 5% (3). the prognosis depends on early diagnosis and treatment.19883265252
gardnerella vaginalis: diagnosis and management. 19883267158
establishing a correct diagnosis of vulvovaginal infection.vulvovaginitis is a common problem and a prime reason for physician consultation. the organism most frequently responsible for vulvovaginal infection is candida albicans, and in a large majority of cases identification of the causative organism and diagnosis are easy to establish. recurrent or persistent infection, however, presents a perplexing diagnostic problem that usually can be solved with the use of appropriate supportive laboratory tests and awareness of the distinguishing features of th ...19883284366
[gardnerella vaginalis in nonspecific vaginitis]. 19873296818
evaluation of the enhanced rapid identification method for gardnerella vaginalis.the enhanced rapid identification method (rim; austin biological laboratories), a micromethod for the identification of gardnerella vaginalis, is based on starch and raffinose fermentation and hippurate hydrolysis. we tested 105 clinical isolates of g. vaginalis with both the rim and standard biochemical tests. the rim agreed with the standard biochemical methods for 96 (91.4%) of the strains; nine isolates which were hippurate hydrolysis positive by standard biochemical tests were hippurate hyd ...19892785533
[nonspecific vaginitis and gardnerella vaginalis: methodological and epidemiological notes on 328 cases].328 samples of vaginal discharges in adult fertile women, aged between 16 and 48, were examined in this trial. after excluding 95 women (39 were affected by vaginal trichomoniasis, 55 whose discharge presented yeasts and 1 affected by gonococcal infection), the authors divided the remaining 233 cases into subjects affected or not affected by nonspecific vaginitis (nsv) only using a gram-stained smear. in the 83 patients affected by nsv, the authors isolated gardnerella vaginalis in the 90.4% of ...19863313504
quantitative studies on the vaginal flora of asymptomatic women and patients with vaginitis and vaginosis.vaginal washings of 22 patients with vaginitis, 11 with vaginosis, and 12 healthy subjects were investigated quantitatively and qualitatively for aerobic and anaerobic bacteria and yeasts. gardnerella vaginalis was recovered from 9 of the vaginitis patients, 7 of the vaginosis patients, and 4 of the asymptomatic subjects. obligate anaerobes were found in 11 of the vaginitis patients, 4 of the vaginosis patients, and none of the control subjects. bacteroides bivius was the anaerobe most frequentl ...19873314265
Displaying items 201 - 300 of 1526