| a rare case of infectious colitis with ulcers in the cecum caused by mycobacterium gordonae. | a 69-year-old female complained of persistent abdominal pain, and annular ulcers and ulcer scars were detected endoscopically in the cecum. pathological findings included caseous granulomas with some langhans giant cells, and ziehl-neelsen staining was negative. mycobacterium gordonae (m. gordonae) was identified by the dna-dna hybridization method and culture (ogawa medium) of biopsy samples from ulcerous cecal lesions. after 6 months of antibiotic therapy, ulcerous cecal lesions were healed, a ... | 2011 | 22041361 |
| isolation of multiple nontuberculous mycobacteria species in the same patients. | during the observation of many patients with nontuberculous mycobacteria (ntm) infections, we have often isolated different ntm species in the same patient. in this study we elucidated the patterns of multiple ntm species isolation. | 2011 | 21873093 |
| species identification of mycobacteria in paraffin-embedded tissues: frequent detection of nontuberculous mycobacteria. | diagnosis of infections caused by mycobacteria, especially nontuberculous mycobacteria still represents a difficult task both in microbiology and pathology. the aim of this study was to determine the frequency of mycobacterial dna detectable by pcr in formalin-fixed paraffin-embedded tissues showing suspicious granulomatous lesions. a total of 190 archival specimens were analyzed, using a nested pcr protocol, which amplifies a fragment of the mycobacterial 65-kda heat-shock protein gene. restric ... | 2005 | 15475934 |
| [nontuberculous mycobacteria in wet areas of a hospital and standard residences]. | from 1998 to 2000, uoeh hospital identified a problem with bronchoscopes which were contaminated with mycobacteria. while conducting research into the problem, we found an extensive amount of nontuberculous mycobacteria (ntm) in wet areas. in this study, we collected a total of 114 samples to screen wet areas such as drains and taps in our hospital and in standard residences. we used the ziehl-neelsen staining method and specific pcr for mycobacteria. as a result, mycobacteria were detected in 9 ... | 2011 | 22259837 |
| detection and identification of mycobacteria in the lisbon water distribution system. | mycobacteria have emerged as a major cause of opportunistic infections. until the present, only a few studies have characterized mycobacteria present in the water distribution system of urban areas. in this study, we characterize these microorganisms in the lisbon water distribution system. our results indicate a high rate of positivities (90.5%) with mainly saprophytic mycobacteria. around 63% of these results belong to strains of mycobacterium gordonae indicating a generalized proliferation of ... | 2005 | 16312965 |
| opportunistic pathogens and elements of the resistome that are common in bottled mineral water support the need for continuous surveillance. | several differences concerning bacterial species, opportunistic pathogens, elements of the resistome as well as variations concerning the cfu/ml counts were identified in some of the five most marketed bottled mineral water from araraquara city, são paulo, brazil. two out of five brands tested were confirmed as potential source of opportunistic pathogens, including mycobacterium gordonae, ralstonia picketti and burkholderia cepacia complex (bcc). a total of one hundred and six isolates were reco ... | 2015 | 25803794 |
| atypical mycobacterial infection among hiv seronegative patients in pondicherry. | the role of non-tuberculous mycobacteria (ntm) as aetiological agents in the causation of lung infections has seldom been systematically studied from india. | 2006 | 16696524 |
| [a case of pulmonary dirofilariasis that required differentiation from nontuberculous mycobacteriosis]. | a 48-year-old man was admitted with an abnormal shadow on a chest x ray. chest x ray and ct revealed a solitary nodule in the right lung field. mycobacterium gordonae was cultured from sputum. tumorectomy by video-assisted thoracoscopic surgery was done for histological diagnosis, and dirofilaria worms accompanied with epithelioid cell granuloma with necrosis were found, while no bacteria were cultured from biopsy specimen, thus we diagnosed dirofilariasis. because a solitary nodule can be cause ... | 2006 | 16780098 |
| non-tuberculous mycobacteria and their surface lipids efficiently induced il-17 production in human t cells. | interleukin-17 (il-17) is produced by a subset of cd4(+) t helper (th) lymphocytes known as th17 cells. in humans, il-1β, enhanced by il-6 and il-23 is crucial for differentiation of these cells. il-17 evokes inflammation and is involved in host defence against microorganisms, although little is known about its role in diseases caused by non-tuberculous mycobacteria. the genus mycobacterium contains both obligate and opportunistic pathogens as well as saprophytes, and the mycobacterial cell enve ... | 2012 | 22939918 |
| the surface lipids of non-tuberculous mycobacteria suppress production of phagocyte activating cytokines in human peripheral blood mononuclear cells. | the genus mycobacterium includes obligate pathogens as well as opportunistic and non-pathogenic species ubiquitous in the environment. mycobacteria have a unique cell wall abundant in lipids. here we investigated cytokine production by human peripheral blood mononuclear cells (pbmc) in response to the opportunistic mycobacteria mycobacterium avium and mycobacterium abscessus, the non-pathogenic mycobacterium gordonae and extracted surface lipids from the three species. the cytokine response elic ... | 2012 | 22497872 |
| utility of gas chromatography for rapid identification of mycobacterial species frequently encountered in clinical laboratory. | over the last years, the clinical importance of mycobacteria has been raised. in this regard, it is important their identification in order to establish either the clinical significance or the appropriate therapy of the disease. biochemical tests are usually time consuming until the report of results, that is why more rapid techniques are needed. as an alternative identification method, we have used a commercially available system for microbial identification based on whole cellular fatty acids ... | 2007 | 17098312 |
| isolation prevalence of pulmonary non-tuberculous mycobacteria in ontario, 1997 2003. | the prevalence of pulmonary non-tuberculous mycobacteria (ntm) infection is reportedly increasing. a study was undertaken of the "isolation prevalence" of pulmonary ntm in ontario, canada between 1997 and 2003 and the frequency of pulmonary ntm "disease". | 2007 | 17311842 |
| a paradigm for the molecular identification of mycobacterium species in a routine diagnostic laboratory. | the aim of this study was to improve the identification of mycobacterium species in the context of a uk teaching hospital. real-time pcr assays were established to enable the rapid differentiation between mycobacterium tuberculosis (mtb) complex and mycobacterium species other than tuberculosis (mott), followed by 16s rrna gene sequencing for the speciation of mott. real-time pcr assays gave comparable results to those from the reference laboratory. the implementation of these pcr assays using a ... | 2007 | 17446280 |
| application of real-time pcr to recognize atypical mycobacteria in archival skin biopsies: high prevalence of mycobacterium haemophilum. | atypical mycobacterial skin infections are difficult to diagnose owing to their aspecific histopathologic presentations and to the presence of few bacteria. therefore, these infections are often not recognized. molecular detection of mycobacterial dna has proven to be useful in clinical samples. the aim of this study was to investigate the incidence of mycobacterial involvement in skin biopsies showing granulomatous inflammation, using real-time polymerase chain reaction (pcr). real-time pcr spe ... | 2007 | 17525676 |
| [a case of pulmonary mycobacterium gordonae infection progressed for no therapy]. | a 68-year-old woman, who had been healthy until this event, presented with a complaint of productive cough since 2000. she went to a neighboring hospital because of bloody sputum in october 2001. although chest radiograph showed abnormal findings then, she received only an expectorant and cough remedy. she consulted us complaining of dyspnea on exertion in april 2005. chest radiograph revealed cavity formation, bronchiectasis and a nodular shadow, and her condition had deteriorated. microbiologi ... | 2007 | 17554990 |
| increased frequency of nontuberculous mycobacteria detection at potable water taps within the united states. | nontuberculous mycobacteria (ntms) are environmental microorganisms that can cause infections in humans, primarily in the lung and soft tissue. the prevalence of ntm-associated diseases is increasing in the united states. exposure to ntms occurs primarily through human interactions with water (especially aerosolized). potable water from sites across the u.s. was collected to investigate the presence of ntm. water from 68 taps was sampled 4 times over the course of 2 years. in total, 272 water sa ... | 2015 | 25902261 |
| nontuberculous mycobacterial peritonitis in peritoneal dialysis patients. | while nontuberculous mycobacterial peritonitis is uncommon among peritoneal dialysis (pd) patients, these infections have serious consequences. they present a significant diagnostic and therapeutic challenge for clinicians. diagnosis can be delayed due to the slow growth rate of some mycobacterial species. these organisms can also be overlooked when adequate culture media are not used in the microbiological evaluation process. the choice of antimicrobial therapy depends upon isolation and specia ... | 2007 | 17555495 |
| fish gambler's tenosynovitis caused by mycobacterium marinum: environmental investigation of a fishing pond in southern taiwan. | we describe a patient with mycobacterium marinum tenosynovitis associated with a fish spine injury acquired at a gambling fishing pond in southern taiwan and identify the source of the infection. m. marinum was isolated from fishing ponds and underground water and wastewater at the site. the isolates shared the same pulsed-field gel electrophoresis pattern as the patient. m. marinum was not detected in 54 samples obtained from 27 fish. mycobacterium gordonae was isolated from 24 samples collecte ... | 2007 | 17572037 |
| nontuberculous mycobacteria in respiratory samples from patients with pulmonary tuberculosis in the state of rondônia, brazil. | the main cause of pulmonary tuberculosis (tb) is infection with mycobacterium tuberculosis (mtb). we aimed to evaluate the contribution of nontuberculous mycobacteria (ntm) to pulmonary disease in patients from the state of rondônia using respiratory samples and epidemiological data from tb cases. mycobacterium isolates were identified using a combination of conventional tests, polymerase chain reaction-based restriction enzyme analysis of hsp65 gene and hsp65 gene sequencing. among the 1,812 ca ... | 2013 | 23827995 |
| total synthesis of an antigenic heptasaccharide motif found in the cell-wall lipooligosaccharide of mycobacterium gordonae strain 989. | an antigenic heptasaccharide motif of the cell-wall glycolipid of mycobacterium gordonae strain 989 has been synthesized in a linear fashion by using a general glycosylation condition and minimum number of protecting group manipulation. all suitably protected monosaccharide intermediates were prepared from commercially available reducing sugars following some novel methodologies recently developed in our laboratory. most of the synthetic intermediates were obtained as solid compounds in excellen ... | 2008 | 18283538 |
| unusual infection in the foot of a barefoot gardener. | we report a case of an unusual and unsuspected chronic infection creating a soft-tissue mass in the foot of a 35-year-old woman. the causative agent, mycobacterium gordonae, is usually encountered as a laboratory contaminant. only rarely does it manifest as a clinical infection. the patient's presumed predisposing risk factor was a history of barefoot gardening. an iatrogenic source, corticosteroid injections, was also considered. | 2008 | 18685052 |
| mycobacterial infection masquerading as cutaneous sarcoidosis. | granulomatous skin inflammation can be secondary to a number of infectious and noninfectious aetiologies. although the presence of mycobacterium gordonae is commonly thought to reflect environmental contamination, infection can occur even in immunocompetent hosts. proper diagnostic evaluation of granulomatous inflammation of the skin is essential in order to optimize treatment recommendations. | 2009 | 19040506 |
| mortality after respiratory isolation of nontuberculous mycobacteria: a comparison of patients who did and did not meet disease criteria. | the mortality of patients with respiratory tract isolates of nontuberculous mycobacteria (ntm) and their risk factors for death are not well described . | 2017 | 28387532 |
| drug susceptibility of rapid and slow growing non-tuberculous mycobacteria isolated from symptomatics for pulmonary tuberculosis, central india. | non-tuberculous mycobacteria (ntm) are emerging as important pathogens. their treatment also differs from that of mycobacterium tuberculosis. in india, any datum on them is scarce as species identification and drug susceptibility are not performed in most laboratories. susceptibility also differs from one geographic area to another, and in our country, there are no data even to guide the clinicians to start treatment empirically. | 2017 | 27934821 |
| environmental mycobacteria from alpine and subalpine habitats. | abstract mycobacteria were isolated from a variety of materials such as soil, peat, humus, tufa, sphagnum, and wood, collected in alpine and subalpine habitats. mycobacteria, including mycobacterium kansasii, mycobacterium malmoense, mycobacterium szulgai, mycobacterium gordonae, mycobacterium terrae, mycobacterium chelonae, and mycobacterium fortuitum were recovered from 69 of 81 (85%) samples. all of the isolates were recovered on medium incubated at 20 and 30 degrees c. none were recovered if ... | 2004 | 19712284 |
| [radiographic findings of pulmonary nontuberculous mycobacteriosis other than mycobacterium avium complex]. | almost all nontuberculous mycobacteria (ntm) cause opportunistic infection. therefore, the radiographic findings of ntm have a tendency of nonspecific patterns modifying the predisposing conditions or diseases and we could not extract species specific radiographic characterizations in that situations. in this review, the ntm cases without predisposing conditions or diseases are submitted essentially. mycobacterium kansasii cases show more or less the same patterns with tb cases. mycobacterium fo ... | 2009 | 19764463 |
| hospital water filters as a source of mycobacterium avium complex. | bronchoscopes and the filters used for washing them were found to yield high numbers of mycobacterium avium isolates sharing the same repetitive sequence-based pcr (rep-pcr) fingerprint pattern as m. avium isolates recovered from patient samples collected by bronchoscopy. water and biofilm samples collected from the bronchoscopy preparation laboratory yielded m. avium, mycobacterium intracellulare, mycobacterium malmoense and mycobacterium gordonae. several m. avium and m. intracellulare isolate ... | 2010 | 20595399 |
| detection of mycobacterium tuberculosis complex and mycobacterium gordonae on the same portable surface plasmon resonance sensor. | in the present study, we have developed a specific detection system for mycobacterium tuberculosis complex (mtb complex) and mycobacterium gordonae by using a commercially available spr based portable-multichannel sensor system. the probe single-strand oligodeoxynucleotides (probe-ssodns), which also contain suitable spacer arms, against the target characteristic sequence (target-ssodns) of both species were selected and synthesized. the spr sensors were prepared by direct coupling of thiolated ... | 2010 | 20667437 |
| mycobacterium shinjukuense sp. nov.; a slowly growing, nonchromogenic species isolated from human clinical specimens. | seven isolates of a slowly growing, nonchromogenic mycobacterium species were isolated from sputum and bronchial lavage fluid samples from elderly patients in different regions of japan. colony morphology, combined with positive results in the tests for tween hydrolysis, catalase at 68°c, semiquantitative catalase, nitrate reductase, and pyrazinamidase, and negative results in the tests for urease, and arylsulfatase distinguished these isolates from related nontuberculous species. the mycolic ac ... | 2010 | 20833878 |
| trinucleotide repeat sequence-based pcr as a potential approach for genotyping mycobacterium gordonae strains. | diseases that are caused by non-tuberculous mycobacteria (ntm) continue to pose difficult clinical problems, and the epidemiological aspect of ntm-caused diseases is of great importance. in the case of mycobacterium gordonae there is no adequate genotyping scheme. here we present a potential rapid and reproducible genetic assay that uses trinucleotide repeat sequence-based pcr (trs-pcr) for genotyping m. gordonae. the proposed method constitutes a useful single-primer pcr screen for genotyping t ... | 2011 | 21276824 |
| disseminated mycobacterium gordonae infection in an immunocompetent host. | mycobacterium gordonae is a slow-growing mycobacterium that is the least pathogenic of the mycobacteria. infection with m. gordonae is most commonly reported in immunocompromised patients. we present a rare case of m. gordonae infection in an immunocompetent individual. a 37-year-old woman was found to have a pulmonary nodule in the left upper lobe. the patient denied any respiratory symptoms, including cough, sputum production, fever, chest pain, or shortness of breath. the patient was a lifeti ... | 2011 | 21566466 |
| granulomatous hypophysitis by mycobacterium gordonae in a non hiv-infected patient. | lymphocytic or granulomatous hypophysitis is a rare entity with a difficult diagnosis. our objective was to report a patient with non-tuberculous granulomatous hypophysitis. an hiv-negative 45-year old man with confusional state, subacute ophthalmoplegia, and clinical and laboratory findings of panhypopituitarism was seen in the emergency unit. a cranial mri showed a sellar mass suggestive of hypophysitis. after an unsuccessful attempt with steroids and antituberculous drugs the patient died. po ... | 2009 | 21577355 |
| serodiagnosis of environmental mycobacterial infections. | to demonstrate the usefulness of enzyme-linked immunosorbent assay for serodiagnosis of mycobacterioses due to environmental mycobacteria we utilized a panel of glycolipid antigens selective for mycobacterium avium-intracellulare, mycobacterium kansasii, mycobacterium xenopi, mycobacterium scrofulaceum and mycobacterium gordonae. the levels of circulating antibodies were determined against the environmental mycobacteria, and mycobacterium tuberculosis in human immunodeficiency virus-negative and ... | 2011 | 21641939 |
| mycobacterium marinum infections in humans and tracing of its possible environmental sources. | the low frequency of nontuberculous mycobacterial infections, nonspecific symptoms for individual mycobacteria, and the lack of specific identification methods could alter correct diagnosis. this study presents a combined microbiology and molecular-based approach for mycobacterium marinum detection in four aquarists with cutaneous mycobacterial infection. simultaneously, ecology screening for m. marinum presence in the aquarists' fish tanks was performed. a total of 38 mycobacterial isolates o ... | 2011 | 22182182 |
| disseminated mycobacterium gordonae infection in a child with cystic fibrosis. | a 4-year-old girl with cystic fibrosis (cf) presented with unrelenting pyrexia commencing shortly after flushing of the central venous catheter (cvc). mycobacterium gordonae was subsequently isolated from bronchoalveolar lavage, gastric washings, and lung biopsy. while this case most likely represents central line infection by a non-tuberculous mycobacterial (ntm) species, it is difficult to state this definitively in the absence of positive cultures from the cvc. we suggest that infection with ... | 2011 | 22028301 |
| [identification of mycobacterium spp. isolates using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (maldi-tof ms)]. | matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (maldi-tof ms) has recently been widely used in diagnostic microbiological laboratories. it is a cheap and rapid method for the identification of bacteria and micromycetes. apart from this purpose, it is also used for the detection of antibiotic resistance mechanisms. it has the potential to be extended for other purposes in microbiology. the aim of this study was to validate maldi-tof ms for the identification of mycob ... | 2014 | 25412483 |
| pulmonary non-tuberculous mycobacteria in a general respiratory population. | the prevalence of non-tuberculous mycobacterium (ntm) appears to be increasing. much of the experience in the literature about this emerging organism comes from specialised units or populations such as cystic fibrosis patients. we, therefore, aim to evaluate the experience in a general respiratory population of dealing with patients with positive culture of ntm. we did a retrospective review of medical notes of general respiratory patients from whom ntm were isolated from january 2007 to july 20 ... | 2014 | 25226715 |
| non-tuberculous mycobacterial infection among lung transplant recipients: a 15-year cohort study. | the incidence of infection with non-tuberculous mycobacteria (ntm) after lung transplant is insufficiently defined. data on the impact of ntm infection on lung transplant survival are conflicting. | 2012 | 22676720 |
| mycobacteria in aquarium fish: results of a 3-year survey indicate caution required in handling pet-shop fish. | fish are commonly infected with non-tuberculous mycobacteria (ntm), which should be regarded as potential pathogens when handling aquarium fish and equipment. this study examined 107 aquarium fish from pet shops. cultivation of the fish samples using different selective media was conducted for identification of ntm. isolates were identified using the genotype mycobacterium common mycobacteria and additional species assays, sequencing of the 16s rrna and rpob genes, and real-time pcr assay for id ... | 2016 | 27747884 |
| diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems. | in this study, to give insight into the bacterial diversity of biofilms from full-scale drinking water distribution systems (dwdss), the bacterial community compositions of biofilms from two urban dwdss (guangzhou and beijing, china) were determined using a 16s rrna gene library technique. meanwhile, the occurrence and diversity of mycobacteria were also analyzed by a mycobacterium -specific hsp gene assay. the biofilms from the full-scale dwdss have complex bacterial populations. proteobacteria ... | 2012 | 22356469 |
| [identification of mycobacteria isolated from aids patients]. | obstract: to identify mycobacteria isolated from aids patients and to evaluate the diagnostic value of mbp64 antigen colloidal gold diagnostic kit in distinguishing mycobacterium tuberculosis(mtb) and nontuberculous mycobacteria (ntm). methods: a total of 140 mycobacteria isolates from aids patients were collected and identified by using 16srdna pcr assay as well as immune colloidal gold technique (mbp64 antigen colloidal gold diagnostic kit). the sensitivity and specificity of colloidal gold me ... | 2016 | 27677657 |
| [a case of pulmonary mycobacterium gordonae infection diagnosed by gastric juice culture and successfully treated with multidrug chemotherapy]. | in september 2008, a 60-year-old woman presented to our hospital with a complaint of bloody sputum; she was healthy until this event. chest computed tomography scan revealed a cavity, nodular shadows, and bronchiectasis in the left upper lobe and in the left and right middle lobes. acid-fast bacilli were detected 2 times on gastric juice culture and mycobacterium gordonae was identified on biochemical study. no active chemotherapy was administered because the discharge of this strain was conside ... | 2012 | 23367832 |
| cooccurrence of free-living amoebae and nontuberculous mycobacteria in hospital water networks, and preferential growth of mycobacterium avium in acanthamoeba lenticulata. | the incidence of lung and other diseases due to nontuberculous mycobacteria (ntm) is increasing. ntm sources include potable water, especially in households where ntm populate pipes, taps, and showerheads. ntm share habitats with free-living amoebae (fla) and can grow in fla as parasites or as endosymbionts. fla containing ntm may form cysts that protect mycobacteria from disinfectants and antibiotics. we first assessed the presence of fla and ntm in water and biofilm samples collected from a ho ... | 2013 | 23475613 |
| extraordinary solute-stress tolerance contributes to the environmental tenacity of mycobacteria. | mycobacteria are associated with a number of well-characterized diseases, yet we know little about their stress biology in natural ecosystems. this study focuses on the isolation and characterization of strains from yellowstone national park (ynp) and glacier national park (gnp; usa), the majority of those identified were mycobacterium parascrofulaceum, mycobacterium avium (ynp) or mycobacterium gordonae (gnp). generally, their windows for growth spanned a temperature range of > 60 °c; selected ... | 2015 | 26059202 |
| detection of pathogenic and non-pathogenic bacteria in drinking water and associated biofilms on the crow reservation, montana, usa. | private residences in rural areas with water systems that are not adequately regulated, monitored, and updated could have drinking water that poses a health risk. to investigate water quality on the crow reservation in montana, water and biofilm samples were collected from 57 public buildings and private residences served by either treated municipal or individual groundwater well systems. bacteriological quality was assessed including detection of fecal coliform bacteria and heterotrophic plate ... | 2015 | 25796498 |
| plasma contributes to the antimicrobial activity of whole blood against mycobacterium tuberculosis. | the whole blood model for infection has proven useful to analyze the immunological response to mycobacterium tuberculosis, because it exerts a significant antimicrobial activity. although this activity has been generally assumed to be cellular, we have found that the leukocyte fraction of blood from healthy volunteers did not kill the bacilli. we have discovered that plasma was responsible for a large proportion, but not all, of the antimicrobial activity. furthermore, infected monocytes control ... | 2016 | 27554054 |
| analysis of pulmonary non-tuberculous mycobacterial infections after lung transplantation. | non-tuberculous mycobacteria (ntm) are important pathogens in lung transplant recipients. this study describes the spectrum of ntm respiratory tract infections and examines the association of ntm infections with lung transplant complications. | 2016 | 27368989 |
| analytical and clinical evaluation of the epistem genedrive assay for detection of mycobacterium tuberculosis. | the epistem genedrive assay rapidly detects the mycobacterium tuberculosis omplex from sputum and is currently available for clinical use. however, the analytical and clinical performance of this test has not been fully evaluated. the analytical limit of detection (lod) of the genedrive pcr amplification was tested with genomic dna; the performance of the complete (sample processing plus amplification) system was tested by spiking m. tuberculosismc(2)6030 cells into distilled water andm. tubercu ... | 2016 | 26865685 |
| utility of the maldi-tof ms method to identify nontuberculous mycobacteria. | in comparison to the conventional real-time polymerase chain reaction method (pcr method) or the dna-dna hybridization method (ddh method), the utility of ntm identification by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) method has seldom been reported. in this study, 75 clinical ntm isolates from our hospital between april 2013 and july 2014 were identified and analyzed using pcr, ddh, and maldi-tof ms methods, and the results for the maldi-to ... | 2016 | 26603481 |
| [identification of mycobacteria isolated from aids patients]. | obstract: to identify mycobacteria isolated from aids patients and to evaluate the diagnostic value of mbp64 antigen colloidal gold diagnostic kit in distinguishing mycobacterium tuberculosis(mtb) and nontuberculous mycobacteria (ntm). | 2016 | 27651187 |
| epidemiology of nontuberculous mycobacteria isolations among central north carolina residents, 2006-2010. | nontuberculous mycobacteria (ntm) are environmental mycobacteria associated with a range of infections. reports of ntm epidemiology have primarily focused on pulmonary infections and isolations, however extrapulmonary infections of the skin, soft tissues and sterile sites are less frequently described. | 2016 | 26997636 |
| performance of a tuberculosis serologic assay in various patient populations. | detection of the humoral response to diagnose active tuberculosis has had varied success. we sought to further characterize the performance of a commercial serologic assay (active tbdetect igg elisa; inbios international, seattle, wa), which had demonstrated promising results in prior studies. | 2015 | 26185308 |
| utility of rpob gene sequencing for identification of nontuberculous mycobacteria in the netherlands. | in the netherlands, clinical isolation of nontuberculous mycobacteria (ntm) has increased over the past decade. proper identification of isolates is important, as ntm species differ strongly in clinical relevance. most of the currently applied identification methods cannot distinguish between all different mycobacterium species and complexes within species. rpob gene sequencing exhibits a promising level of discrimination among rapidly and slowly growing mycobacteria, including the mycobacterium ... | 2014 | 24808238 |
| surveillance and molecular characterization of non-tuberculous mycobacteria in a hospital water distribution system over a three-year period. | a three-year surveillance of non-tuberculous mycobacteria (ntm) in a hospital water distribution system was conducted at a facility located in southern alberta. ntm was not present in any intake water samples, but was found in 106/183 (58%) of endpoint samples across 15 sites over the study period. two different species of ntm were identified, mycobacterium gordonae (88/183) and mycobacterium avium (34/183); with only one strain of each m. gordonae and m. avium found. given the sensitive nature ... | 2014 | 24746232 |
| identification of non-tuberculous mycobacteria from clinical samples. | non-tuberculous mycobacterial infections cause morbidity worldwide. ntm are considered opportunistic pathogens, and several species have been associated with human disease which has typically pulmonary, skin and soft tissue, lymphatic or disseminated presentation. this study evaluated the distribution of non-tuberculous mycobacteria in sardinia. mycobacterium avium, mycobacterium gordonae and mycobacterium xenopi were frequently found. our results agreed with literature data both for the frequen ... | 2013 | 24177303 |
| identification of non-tuberculous mycobacteria by real-time pcr coupled with a high-resolution melting system. | non-tuberculous mycobacteria (ntm) are increasingly important opportunistic pathogens responsible for a variety of clinical diseases. the aim of this study was to evaluate a novel technique, real-time pcr coupled with high-resolution melting analysis (real-time pcr-hrma), for ntm identification. two pairs of unique primers targeted to the 16s rrna gene and the 16s-23s internal transcribed spacer region were selected for further evaluation. a total of 149 mycobacterial clinical isolates were subj ... | 2012 | 22493281 |
| detection and identification of globally distributed mycobacterial fish pathogens in some ornamental fish in india. | mycobacteriosis is a progressive disease of a wide range of wild and captive, marine and freshwater fish species. conventional detection of fish mycobacteria is based on histopathology, culture, and biochemical characteristics. the present study analyzed the occurrence of mycobacteria in clinically ill ornamental fish of different species, from different places of india. in first group, 60 fish were examined for presence of granulomatous inflammation and acid-fast bacteria. thirty-eight (63.34 % ... | 2013 | 23358914 |
| distribution of non-tuberculosis mycobacteria strains from suspected tuberculosis patients by heat shock protein 65 pcr-rflp. | the genus mycobacterium contains more than 150 species. non-tuberculosis mycobacteria (ntm) often cause extrapulmonary and pulmonary disease. mycobacteria detection at species level is necessary and provides useful information on epidemiology and facilitates successful treatment of patients. this retrospective study aimed to determine the incidence of the ntm isolates and mycobacterium tuberculosis (mtb) in clinical specimens collected from iranian patients during february 2011-december 2013, by ... | 2017 | 28855835 |
| therapy-resistant septic olecranon bursitis due to mycobacterium gordonae. | septic olecranon bursitis due to atypical mycobacteria is rare. an insidious beginning can delay diagnosis and treatment. antibacterial therapy recommendations are not well-defined for bursitis caused by atypical mycobacteria. we present a rare case of olecranon bursitis caused by mycobacterium gordonae, reporting our experiences regarding pathogen identification and antibiotic therapy, which differs from regimes used in common septic bursitis mostly caused by staphylococcus aureus. | 2016 | 27892398 |
| first draft genome sequence of a mycobacterium gordonae clinical isolate. | here, we report the first draft genome sequence of the clinically relevant species mycobacterium gordonae the clinical isolate mycobacterium gordonae 14-8773 was obtained from the sputum of a patient with mycobacteriosis. | 2016 | 27365356 |
| pseudo-outbreak of mycobacterium gordonae in a teaching hospital: importance of strictly following decontamination procedures and emerging issues concerning sterilization. | aim of this study was to investigate a pseudo-outbreak of mycobacterium gordonae analyzing isolates detected from clinical and environmental samples. mycobacterium gordonae was detected in 7 out of 497 broncho-alveolar lavage (bal) samples after bronchoscopy procedure in patients admitted to a teaching hospital between january and april 2013. during this pseudo-outbreak clinical, epidemiological, environmental and molecular investigations were performed. none of the patients met the criteria for ... | 2016 | 26922983 |
| false-positive quantiferon tb-gold test due to mycobacterium gordonae. | we report a case of quantiferon tb-gold conversion associated to mycobacterium gordonae in an elderly male from an assisted living facility without known risk factors for tuberculosis. this knowledge of environmental mycobacteria causing positive quantiferon assays is important to avoid unnecessary treatment of false-positive latent tuberculosis, especially in the absence of well-established positive predictive value of quantiferon conversion. | 2016 | 26827092 |
| mycobacterium gordonae-induced humidifier lung. | nontuberculous mycobacteria are well known to be a cause of hot tub lung, however, to our knowledge, there exists no case report of humidifier lung induced by mycobacteria. | 2015 | 26420433 |
| first total synthesis of trehalose-containing branched oligosaccharide ose-1 of mycobacterium gordonae (strain 990). | the first total synthesis of the branched oligosaccharide ose-1 of mycobacterium gordonae (strain 990) is reported. an intramolecular aglycon delivery approach was used for constructing the desymmetrized 1,1'-α,α-linked trehalose moiety. a [3+2] glycosylation of the trisaccharide donor and trehalose acceptor furnished the right hand side pentasaccharide. regioselective o3 glycosylation of l-rhamnosyl 2,3-diol allowed expedient synthesis of the left hand side tetrasaccharide. the nonasaccharide w ... | 2015 | 26251305 |
| diagnosis of nontuberculous mycobacterial infections. | the nontuberculous mycobacteria (ntm) are typically environmental organisms residing in soil and water. although generally of low pathogenicity to humans, ntm can cause a wide array of clinical diseases; pulmonary disease is most frequent, followed by lymphadenitis in children, skin disease by m. marinum (particularly in fish tank fanciers), and other extrapulmonary or disseminated infections in severely immunocompromised patients. of the >140 ntm species reported in the literature, 25 species h ... | 2013 | 23460010 |
| evaluation of a method to detect mycobacterium bovis in air samples from infected eurasian badgers (meles meles) and their setts. | environmental air sampling was evaluated as a method to detect the presence of m. bovis in the vicinity of infected badgers and their setts. airborne particles were collected on gelatine filters using a commercially available air sampling instrument and tested for the presence of m. bovis using bacteriological culture and real-time pcr. the sensitivity of bacteriological culture was broadly similar to that of real-time pcr when testing samples artificially spiked with m. bovis. sampling was unde ... | 2013 | 23384280 |
| mycobacterium gordonae skin infection in an immunocompetent child. | mycobacterium gordonae is a slow growing, pigmented, non-tuberculous mycobacterium (ntm). it is commonly associated with environmental contamination of clinical specimens but it is also a recognized pathogen in immunocompromised hosts. we describe an immunocompetent child with a spontaneously occurring skin ulcer on the face caused by m. gordonae infection. | 2017 | 28114153 |
| clinical and microbiological features of definite mycobacterium gordonae pulmonary disease: the establishment of diagnostic criteria for low-virulence mycobacteria. | although mycobacterium gordonae isolation from respiratory samples is usually regarded as contamination, m. gordonae can cause definite pulmonary disease. the establishment of a standard diagnostic criteria of pulmonary disease that is caused by this low virulence mycobacterium is obviously necessary. | 2015 | 26209978 |
| mycobacteria in water used for personal hygiene in heavy industry and collieries: a potential risk for employees. | environmental mycobacteria (em) constitute a health risk, particularly for immunocompromised people. workers in heavy industry and in collieries represent an at-risk group of people as their immunity is often weakened by long-term employment in dusty environments, frequent smoking and an increased occurrence of pulmonary diseases. this study was concerned with the presence of em in non-drinking water used for the hygiene of employees in six large industrial companies and collieries. over a perio ... | 2015 | 25749321 |
| disseminated mycobacterium gordonae and mycobacterium mantenii infection with elevated anti-ifn-γ neutralizing autoantibodies. | a case of disseminated nontuberculous mycobacteria(l) (ntm) infection in a patient with positive neutralizing anti-interferon-γ (ifn-γ) autoantibodies involving bone, bronchus, systemic lymph nodes, and skin is reported. the causative ntms were two different strains: mycobacterium gordonae, which rarely causes true disease, and mycobacterium mantenii, which is extremely rare. anti-mycobacterial treatment successfully ameliorated all disseminated lesions. although the concentration of anti-ifn-γ ... | 2015 | 25735882 |
| nosocomial pseudo-outbreak of mycobacterium gordonae associated with a hospital's water supply contamination: a case series of 135 patients. | nontuberculous mycobacteria (ntm) are opportunistic pathogens found in natural and human-engineered waters. in 2009, a relative increase in the isolation of mycobacterium gordonae from pulmonary samples originating from general hospital zabok was noted by the national mycobacteria reference laboratory. an epidemiological survey revealed a contamination of the cold tap water with m. gordonae and guidelines regarding sputum sample taking were issued. in addition, all incident cases of respiratory ... | 2015 | 25719472 |
| pseudo-outbreak of mycobacterium gordonae following the opening of a newly constructed hospital at a chicago medical center. | objective to identify the source of a pseudo-outbreak of mycobacterium gordonae design outbreak investigation. setting university hospital in chicago, ilinois. patients hospital patients with m. gordonae-positive clinical cultures. methods an increase in isolation of m. gordonae from clinical cultures was noted immediately following the opening of a newly constructed hospital in january 2012. we reviewed medical records of patients with m. gordonae-positive cultures collected between january and ... | 2015 | 25633003 |
| shedding light on host niches: label-free in situ detection of mycobacterium gordonae via carotenoids in macrophages by raman microspectroscopy. | macrophages are the primary habitat of pathogenic mycobacteria during infections. current research about the host-pathogen interaction on the cellular level is still going on. the present study proves the potential of raman microspectroscopy as a label-free and non-invasive method to investigate intracellular mycobacteria in situ. therefore, macrophages were infected with mycobacterium gordonae, a mycobacterium known to cause inflammation linked to intracellular survival in macrophages. here, we ... | 2015 | 25488726 |
| mycobacterium gordonae in patient with facial ulcers, nosebleeds, and positive t-spot.tb test, china. | mycobacterium gordonae is often regarded as a weak pathogen that only occasionally causes overt disease. we report a case of m. gordonae infection in the facial skin, nasal mucosa, and paranasal sinus in an immunocompetent patient and review previous cases. the t-spot.tb test might be useful in diagnosing such cases. | 2017 | 28628445 |
| death caused by possible unrecognized (too late recognized) mycobacterium gordonae infection in a patient with angioimmunoblastic t-cell lymphoma. | here, we present possible death caused by mycobacterium gordonae infection in a patient with angioimmunoblastic t-cell lymphoma. our patient was severely immunocompromised in whom we suspect to an infection, but we did not have isolates until she died. after she died, we received a positive sputum culture of m. gordonae. we conclude that when having severely immunocompromised patients with suspicion of infection but without isolates we should always consider the saprophytic mycobacteria. these m ... | 2017 | 28559527 |
| mycobacterium gordonae skin infection in an immunocompetent child. | mycobacterium gordonae is a slow growing, pigmented, nontuberculous mycobacterium. it is commonly associated with environmental contamination of clinical specimens, but it is also a recognized pathogen in immunocompromised hosts. we describe an immunocompetent child with a spontaneously occurring skin ulcer on the face caused by m. gordonae infection. | 2017 | 28403059 |
| chronic postoperative mycobacterium gordonae endophthalmitis in a patient with phakic intraocular lens. | case description of a 34 year old woman with chronic granulomatous anterior uveitis following implantation of a phakic anterior chamber intraocular lens (iol). aqueous humor was processed for bacterial, fungal culture, and pcr analysis. | 2013 | 23978249 |
| isolation of nontuberculous mycobacteria from hospital waters in turkey. | nontuberculous mycobacteria (ntm) are ubiquitous in hot and cold water distribution systems. with molecular typing methods it was shown that water can be the source of colonization and infection with ntm. the aim of our study was the investigation of ntm in hot and cold water samples taken from various departments of two hospitals in istanbul, turkey. totally, 160 water samples were examined. the temperature, ph, and free chlorine levels of water samples were measured between 10-41 °c, 6.78-7.98 ... | 2013 | 23509870 |
| spectrum of non-tuberculous mycobacteria identified using standard biochemical testing vs. 16s sequencing. | non-tuberculous mycobacterial isolates from gold miners were speciated using standard biochemical testing (sbt) and 16s rdna sequencing. of 237 isolates tested, sbt identified 126, compared with all 237 identified using sequencing. of 111 isolates unspeciated by sbt but identified by sequencing, 38 (34.2%) were identified as mycobacterium gordonae and 8 (7.2%) were new species. of 126 isolates speciated by both methods, 37 were discordant, with 14/17 m. gordonae isolates incorrectly identified a ... | 2013 | 23228479 |
| mycobacterium gordonae pulmonary infection in an immunocompetent adult. | nontuberculous mycobacteria are non-communicable organisms and currently there are over 125 species. nontuberculous mycobacteria are usually recovered from the environment and can cause disease through respiratory, cutaneous, parenteral and gastrointestinal exposure. | 2010 | 22624142 |
| circulating follicular helper t cells presented distinctively different responses toward bacterial antigens in primary biliary cholangitis. | primary biliary cholangitis (pbc) is a chronic and progressive cholestatic liver disease with unknown causes. the initiation of pbc is associated with bacterial infections and abnormal immune correlates, such as the presence of self-reactive anti-mitochondrial antibodies and shifted balance of t cell subsets. in particular, the cd4(+)cxcr5(+) follicular helper t (tfh) cells are highly activated in pbc patients and are significantly associated with pbc severity, but the underlying reasons are unk ... | 2017 | 28806642 |
| repeated acute kidney injury associated with mycobacterium gordonae infection. | mycobacterium gordonae is a nontuberculous mycobacterium widely distributed in the environment. although m. gordonae is not usually pathogenic and glomerular lesions due to m. gordonae are very rare, infection has been reported in both immunocompromised patients and healthy persons. we report a case of acute kidney injury (aki) in which m. gordonae was ultimately identified as the cause. a 70-year-old man was admitted to our hospital because of fever, polyarthritis, and aki. he was a hepatitis b ... | 2015 | 28509088 |
| species-specific risk factors, treatment decisions, and clinical outcomes for laboratory isolates of less common nontuberculous mycobacteria in washington state. | nontuberculous mycobacteria (ntm) are a diverse group of environmental organisms that infrequently cause human disease. understanding of the epidemiologic and clinical characteristics associated with ntm disease is needed to refine diagnostic and treatment strategies, particularly among the less commonly isolated species. | 2017 | 28387523 |
| spatio-temporal study of environmental nontuberculous mycobacteria isolated from wardha district in central india. | during the last two decades, nontuberculous mycobacteria (ntm) have gained in importance but there is still a paucity of data, particularly for environmental isolates. we studied, over a period of two years, the spatio-temporal features of ntm isolates obtained from different environmental sources in wardha district, india. a total of 1398 samples (699 each of soil and water) were tested and 170 (12.2%) yielded ntm isolates, including 123 from soil and 47 from water samples. out of 170 ntm isola ... | 2017 | 28836034 |
| pulmonary infection with caseating mediastinal lymphadenitis caused by mycobacterium gordonae. | it is often difficult to discern true mycobacterial infection from colonization due to mycobacterium gordonae (m. gordonae) since this organism is ubiquitous and is commonly an innocuous saprophyte. this study reports a rare case of caseating hilar adenopathy and pulmonary disease caused by m. gordonae in a patient with chronic obstructive pulmonary disease (copd) and rheumatoid arthritis (ra) on maintenance steroids and methotrexate. pathologic exam and cultures of lymph node excision biopsy an ... | 2014 | 26786493 |
| mycobacterium paragordonae sp. nov., a slowly growing, scotochromogenic species closely related to mycobacterium gordonae. | a previously undescribed, slowly growing, scotochromogenic mycobacterial strain (49061(t)) was isolated from a patient with pulmonary infections during the hsp65-sequence-based identification of korean clinical isolates. its 16s rrna gene sequence was unique and the phylogenetic analysis based on 16s rrna gene sequence (1393 bp) placed the organism into the slow-growing mycobacterium group close to mycobacterium gordonae (99.0 % sequence similarity). growth characteristics and acid-fastness also ... | 2014 | 24006480 |