| characterisation of a bifidobacterium strain with acquired resistance to cholate--a preliminary study. | the susceptibility levels against bile salts (ox gall) and sodium cholate of 19 bifidobacterium strains from different origins ranged between 0.125% and 2.0% and 0.05% and 0.4%, respectively. seven of these strains were subsequently selected for the isolation of sodium cholate resistant derivatives by exposure to gradually increasing concentrations of this compound. derivatives resistant to at least 1.2% of cholate were obtained from bifidobacterium bifidum cect 4549 and b. bifidum m6. further a ... | 2003 | 12568759 |
| lactic acid bacteria isolated from dairy products inhibit genotoxic effect of 4-nitroquinoline-1-oxide in sos-chromotest. | antigenotoxic activity against 4-nitroquinoline-1-oxide (4-nqo) of lactic acid bacteria isolated from commercial dairy products was studied using sos-chromotest. the supernatants from bacteria-genotoxin co-incubations in general exhibited a strong suppression on sos-induction produced by 4-nqo on the tester organism escherichia coli pq37 (sfia::lacz). high genotoxicity inhibition (>75%) was found for 31/67 of the examined bacteria and the maximum values of some strains within the species were as ... | 2002 | 12583707 |
| antimicrobial properties of commercial annatto extracts against selected pathogenic, lactic acid, and spoilage microorganisms. | annatto preparations are used to impart distinctive flavor and color to foods and are a primary colorant in dairy foods such as cheese and butter. there are several reports indicating that certain fractions of the annatto plant have biological activities against microorganisms of significance in food fermentation, food preservation, and human health. however, little is reported describing the nature of the antimicrobial compound(s) or their potential presence in commercial annatto colorant prepa ... | 2003 | 12801012 |
| in vitro growth responses of bifidobacteria and enteropathogens to bovine and human lactoferrin. | a series of in vitro experiments was performed to test the ability of bovine and human lactoferrin to influence the growth of the gram-positive probiotic bacteria, bifidobacterium bifidum, bifidobacterium infantis, and lactobacillus acidophilus, as well as the gram-negative enteric bacteria, e. coli o157:h7 and salmonella typhimurium. none of the lactoferrin preparations stimulated the growth of the tested strains. however, iron-free apo-lactoferrin (bovine and human) and 66% iron-saturated bovi ... | 2003 | 12870790 |
| andean yacon root (smallanthus sonchifolius poepp. endl) fructooligosaccharides as a potential novel source of prebiotics. | the ability of three known probiotic strains (two lactobacilli and one bifidobacterium) to ferment fructooligosaccharides (fos) from yacon roots (smallanthus sonchifolius poepp. endl) was compared to commercial fos in this study. results indicate that lactobacillus acidophilus nrrl-1910, lactobacillus plantarum nrrl b-4496, and bifidobacterium bifidum atcc 15696 were able to ferment yacon root fos. fos consumption apparently depended on the degree of polymerization and the initial fos compositio ... | 2003 | 12926870 |
| [a contribution to the serology of bacterium bifidum]. | | 1952 | 13023975 |
| antigenic and cultural relationships of lactobacillus bifidus and lactobacillus parabifidus. | | 1953 | 13052958 |
| [a half-synthetic culture medium for bacterium bifidum]. | | 1953 | 13103337 |
| [on the growth substance for bacterium bifidum]. | | 1953 | 13103365 |
| [problem of the growth substance of bacterium bifidum]. | | 1953 | 13103386 |
| [the effect of chromobacterium prodigiosum on the growth of bacterium bifidum]. | | 1954 | 13188176 |
| [distinction between bifidibacterium bifidum and lactobacillus acidophilus]. | | 1955 | 13403273 |
| [morphology and biochemistry of bacterium bifidum]. | | 1957 | 13426054 |
| [general remarks on bacillus bifidus in infants]. | | 1959 | 13635068 |
| [problems concerning bacillus bifidus]. | | 1959 | 13635073 |
| [paper chromatographic study of the amino acid content of bacterium bifidum]. | | 1961 | 13742976 |
| [the antibiotically effective principle of bacterium bifidum. in vitro studies]. | | 1960 | 13768575 |
| [contribution to the knowledge of problems relative to bacillus bifidus in the infant]. | | 1960 | 13780446 |
| [the problems of bacillus bifidus]. | | 1960 | 13853641 |
| [further contribution on bacillus bifidus in infants. clinical trials of petuely's "bifidus factor"]. | | 1961 | 13873080 |
| [the significance of bacterium bifidum for the infant]. | | 1962 | 13916832 |
| [acute bacterial enterocolitis in infants. trial treatment with bacillus bifidus]. | | 1964 | 14145293 |
| [on the metabolism of bacterium bifidum (lactobacillus bifidus). v. co2 formation and o2 uptake in different nutrient media]. | | 1965 | 14272282 |
| [on the metabolism of bacterium bifidum (lactobaccilus bifidus). i. co2 formation in tomarelli solution]. | | 1964 | 14297254 |
| [bacterium bifidum, its serological classification and vitamin b1 production]. | | 1965 | 14311250 |
| [on the metabolism of the bacterium bifidum (lactobacillus bifidus). ii. lactic acid formation in tomarelli solution]. | | 1964 | 14316619 |
| [on the metabolism of bacterium bifidum (lactobacillus bifidus). iv. catalase activity in aerobic and anaerobic cultures]. | | 1964 | 14333486 |
| [on the metabolism of bacterium bifidum (lactobacillus bifidus). 3. oxygen uptake in o-potato culture medium]. | | 1964 | 14334645 |
| [sensitivity of bifidibacterium bifidum to 11 antibiotics]. | | 1959 | 14414643 |
| [research on the antagonism between bifidobacterium bifidum and aminogenic intestinal bacteria]. | | 1962 | 14478774 |
| genus- and species-specific pcr primers for the detection and identification of bifidobacteria. | 16srdna-targeted genus- and species-specific pcr primers have been developed and used for the identification and detection of bifidobacteria. these primers cover all of the described species that inhabit the human gut, or occur in dairy products. identification of cultured bifidobacteria using pcr primer pairs is rapid and accurate, being based on nucleic acid sequences. detection of bifidobacteria can be achieved using dna extracted from human faeces as template in pcr reactions. we have found ... | 2003 | 14503690 |
| protection of bifidobacteria encapsulated in polysaccharide-protein gel beads against gastric juice and bile. | bifidobacterium cells were encapsulated in a mixed gel composed of alginate, pectin, and whey proteins. two kinds of capsules were obtained: gel beads without membranes and gel beads with two membranes formed by the transacylation reaction. in vitro studies were carried out to determine the effects of simulated gastric ph and bile salts on the survival of free and encapsulated bifidobacterium bifidum. the protective effects of gel beads without membranes and gel beads coated with two membranes f ... | 2003 | 14627286 |
| survival of lactobacillus acidophilus la-5 and bifidobacterium bifidum bb-02 in white-brined cheese. | the viability of probiotic cultures used as adjunct cultures including bifidobacterium bifidum bb-02 and lactobacillus acidophilus la-5 in white-brined cheese was investigated. the microbiological and biochemical properties of cheeses were assessed throughout 90 days of storage. two inoculum rates for probiotic starters (2.5% and 5.0%, v/v, corresponding to (1.0-1.3)x10(9) cfu/g and (2.0-2.1)x10(9) cfu/g, respectively) were compared with the control cheese. results obtained showed that a higher ... | 2004 | 14630592 |
| relationship between interaction sites in the gut, hydrophobicity, mucosal immunomodulating capacities and cell wall protein profiles in indigenous and exogenous bacteria. | to investigate whether there is a relationship between interaction sites in the gut, hydrophobicity, mucosal immunomodulating capacities and cell wall protein profiles in lactobacilli, bifidobacteria and enterococci. | 2004 | 14723684 |
| binding of bifidobacterium bifidum and lactobacillus reuteri to the carbohydrate moieties of intestinal glycolipids recognized by peanut agglutinin. | we examined binding of bifidobacterium bifidum and lactobacillus reuteri to the carbohydrate moieties of glycolipids extracted from human enterocyte-like caco-2 cells in this study. in binding assays to reference glycolipids of different carbohydrate compositions, b. bifidum eb102 bound strongly to gangliotetraosylceramide (asialo-gm1) and less strongly to gangliotriaosylceramide (asialo-gm2), lactosylceramide and sulfatide. the binding profile of b. bifidum eb102 was almost identical to that of ... | 2004 | 14751691 |
| purification and characterization of three different types of bile salt hydrolases from bifidobacterium strains. | bile salt hydrolases were purified to electrophoretic homogeneity from bifidobacterium bifidum atcc 11863, bifidobacterium infantis kl412, bifidobacterium longum atcc 15708, bifidobacterium longum kl507, and bifidobacterium longum kl515. three different types (a, b, and c) of bile salt hydrolase (bsh) were revealed during the purification study, exhibiting the type-specific characteristics in their electrophoretic migration and elution profiles from anion exchange and hydrophobic interaction chr ... | 2004 | 14762068 |
| [observations on bacterium bifidum]. | | 1950 | 14789079 |
| studies on bacterium bifidum in healthy infants; a clinical bacteriological investigation. | | 1951 | 14829262 |
| probiotic bifidobacteria protect mice from lethal infection with shiga toxin-producing escherichia coli o157:h7. | the anti-infectious activity of probiotic bifidobacteria against shiga toxin-producing escherichia coli (stec) o157:h7 was examined in a fatal mouse stec infection model. stable colonization of the murine intestines was achieved by the oral administration of bifidobacterium breve strain yakult (naturally resistant to streptomycin sulfate) as long as the mice were treated with streptomycin in their drinking water (5 mg/ml). the pathogenicity of stec infection, characterized by marked body weight ... | 2004 | 15039348 |
| management of infant diarrhea with high-lactose probiotic-containing formula. | to study the influence of high-lactose probiotic-containing formula on the course of acute diarrhea, an experiment using a randomized controlled clinical trial with patients having acute diarrhea for 3 days was conducted. one hundred patients were allocated into two groups that were comparable for age, sex, and nutritional status. the test group was administered high-lactose bifidobacterium bifidum-containing formula, while the control group had no high-lactose probiotic until the end of the exp ... | 2003 | 15115098 |
| lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport. | probiotic bacteria have a beneficial effect on intestinal inflammation. in this study, we have examined the effect of lactic acid and commensal gram positive (+) bacteria conditioned media (cm) on tumour necrosis factor alpha (tnf-alpha) release and the mechanisms involved. | 2004 | 15138208 |
| acquired resistance to bile increases fructose-6-phosphate phosphoketolase activity in bifidobacterium. | two bifidobacterium strains with acquired resistance to bile were used in this study. significant differences on membrane-associated protein profiles were found between the bile resistant derivatives and their corresponding original strains. one of the major species detected in one of the resistant derivatives had an apparent denatured molecular mass of approximately 90 kda, and was identified as xylulose-5-phosphate/fructose-6-phosphate phosphoketolase, the key enzyme of bifidobacterium carbohy ... | 2004 | 15158259 |
| bifidobacterial species differentially affect expression of cell surface markers and cytokines of dendritic cells harvested from cord blood. | the gut microbiota may be important in the postnatal development of the immune system and hence may influence the prevalence of atopic diseases. bifidobacteria are the most numerous bacteria in the guts of infants, and the presence or absence of certain species could be important in determining the geographic incidence of atopic diseases. we compared the fecal populations of bifidobacteria from children aged 25 to 35 days in ghana (which has a low prevalence of atopy), new zealand, and the unite ... | 2004 | 15242942 |
| growth responses of cassia obtusifolia toward human intestinal bacteria. | 1,2-dihydroxyanthraquinone (1) was isolated from the seed of cassia obtusifolia through bioassay-guided fractionation. 1,2-dihydroxyanthraquinone strongly inhibited the growth of clostridium perfringens and escherichia coli. structure-activity relationship revealed that 1,4-dihydroxyanthraquinone (2) and 1,8-dihydroxyanthraquinone (3) had strong growth-inhibition against c. perfringens. in growth-promoting activity, 1,2-, 1,4-, and 1,8-dihydroxyanthraquinones exhibited strong growth-promoting ac ... | 2004 | 15261390 |
| molecular cloning and characterization of bifidobacterium bifidum 1,2-alpha-l-fucosidase (afca), a novel inverting glycosidase (glycoside hydrolase family 95). | a genomic library of bifidobacterium bifidum constructed in escherichia coli was screened for the ability to hydrolyze the alpha-(1-->2) linkage of 2'-fucosyllactose, and a gene encoding 1,2-alpha-l-fucosidase (afca) was isolated. the afca gene was found to comprise 1,959 amino acid residues with a predicted molecular mass of 205 kda and containing a signal peptide and a membrane anchor at the n and c termini, respectively. a domain responsible for fucosidase activity (the fuc domain; amino acid ... | 2004 | 15262925 |
| genetic heterogeneity and functional properties of intestinal bifidobacteria. | the aim of the present study was to compare several molecular methods for the identification and genotyping of bifidobacteria, and further to investigate genetic heterogeneity and functional properties of bifidobacterial isolates from intestinal samples of finnish adult subjects. | 2004 | 15281925 |
| [antibacterial effects of water-soluble low-molecular-weight chitosans on different microorganisms]. | low-molecular-weight chitosans with a viscosity-average molecular weight (mv) of 5 to 27 kda and equal degree of deacetylation (dd, 85%) were highly active against pseudomonas aureofaciens, enterobacter agglomerans, bacillus subtilis, and bifidobacterium bifidum 791, causing death of 80 to 100% of cells. an exception to this tendency was escherichia coli, for which the rate of cell death, induced by the 5-kda chitosan, was 38%. the antibacterial effect was manifested as early as 10 min after inc ... | 2004 | 15283332 |
| mining the microbiota of the neonatal gastrointestinal tract for conjugated linoleic acid-producing bifidobacteria. | this study was designed to isolate different strains of the genus bifidobacterium from the fecal material of neonates and to assess their ability to produce the cis-9, trans-11 conjugated linoleic acid (cla) isomer from free linoleic acid. fecal material was collected from 24 neonates aged between 3 days and 2 months in a neonatal unit (erinville hospital, cork, ireland). a total of 46 isolates from six neonates were confirmed to be bifidobacterium species based on a combination of the fructose- ... | 2004 | 15294796 |
| cloning and characterization of the bile salt hydrolase genes (bsh) from bifidobacterium bifidum strains. | biochemical characterization of the purified bile salt hydrolase (bsh) from bifidobacterium bifidum atcc 11863 revealed some distinct characteristics not observed in other species of bifidobacterium. the bsh gene was cloned from b. bifidum, and the dna flanking the bsh gene was sequenced. comparison of the deduced amino acid sequence of the cloned gene with previously known sequences revealed high homology with bsh enzymes from several microorganisms and penicillin v amidase (pva) of bacillus sp ... | 2004 | 15345449 |
| [attempt at a classification of the bacterium bifidum]. | | 1950 | 15437840 |
| anticancerogenic effect of a novel chiroinositol-containing polysaccharide from bifidobacterium bifidum bgn4. | strains of bifidobacteria have many health-promotion effects. whole cells or cytoplasm extracts of bifidobacterium bifidum bgn4, isolated from human feces, inhibited the growth of several cancer cell lines. the polysaccharide fraction (bb-pol) extracted from b. bifidum bgn4 had a novel composition, comprising chiroinositol, rhamnose, glucose, galactose, and ribose. three human colon cancer cell lines were treated with bb-pol: ht-29, hct-116, and caco-2. trypan blue exclusion assay and brdu incor ... | 2004 | 15522499 |
| variation of microbiological and biochemical parameters in the faeces of two healthy people over a 15 day period. | recent years have seen increasing interest in the complex microbial ecosystem of the human gastrointestinal tract. knowledge of its microbial colonists and their beneficial/detrimental activities is important. however, generalized assumptions about the microbial composition of the human gut should be taken with caution until more studies in different human communities have been conducted. the capacity of modulating or inhibiting harmful populations through high doses of beneficial microorganisms ... | 2004 | 15597174 |
| molecular identification of monomeric aspartate racemase from bifidobacterium bifidum. | bifidobacterium bifidum is a useful probiotic agent exhibiting health-promoting properties and contains d-aspartate as an essential component of the cross-linker moiety in the peptidoglycan. to help understand d-aspartate biosynthesis in b. bifidum nbrc 14252, aspartate racemase, which catalyzes the racemization of d- and l-aspartate, was purified to homogeneity and characterized. the enzyme was a monomer with a molecular mass of 27 kda. this is the first report showing the presence of a monomer ... | 2004 | 15606767 |
| microbiological effects of consuming a synbiotic containing bifidobacterium bifidum, bifidobacterium lactis, and oligofructose in elderly persons, determined by real-time polymerase chain reaction and counting of viable bacteria. | because of changes in gut physiology, immune system reactivity, and diet, elderly people are more susceptible to gastrointestinal infections than are younger adults. the gut microflora, which provides a natural defense against invading microorganisms, changes in elderly people with the development of potentially damaging bacterial populations, which may lead to alterations in bacterial metabolism and higher levels of infection. | 2004 | 15614689 |
| synthesis of prebiotic galactooligosaccharides using whole cells of a novel strain, bifidobacterium bifidum ncimb 41171. | a novel strain of bifidobacterium bifidum ncimb 41171, isolated from a faecal sample from a healthy human volunteer and able to express beta-galactosidase activity, was used in synthesis reactions for the production of galactooligosaccharide from lactose. the beta-galactosidase activity of whole bifidobacterial cells showed an optimum activity at ph 6.8-7.0 and 40 degrees c. the transgalactosylation activity of the b. bifidum cells from 50% (w/w) lactose resulted in a galactooligosaccharide mixt ... | 2005 | 15703907 |
| selective growth-inhibiting effects of compounds identified in tabebuia impetiginosa inner bark on human intestinal bacteria. | the growth-inhibiting activity of anthraquinone-2-carboxylic acid and lapachol identified in the inner bark of taheebo, tabebuia impetiginosa, toward 10 human intestinal bacteria was evaluated by using a paper disk diffusion bioassay and compared to those of seven lapachol congeners (1,4-naphthoquinone, naphthazarin, menadione, lawsone, plumbagin, juglone, and dichlone) as well as two commercially available antibiotics, chloramphenicol and tetracycline. anthraquinone-2-carboxylic acid exhibited ... | 2005 | 15713033 |
| safety evaluation of lactobacillus delbrueckii subsp. lactis uo 004, a probiotic bacterium. | lactobacillus delbrueckii subsp. lactis uo 004 was evaluated for its use as a potential probiotic from a safety point of view. the strain did not exhibit mucinolytic or other enzymatic activities that might be detrimental, such as those involving glycosidases (beta-d-glucosaminidase or alpha-d-galactosidase) or arylamidases (factor xa and quimotrypsin-like activities), frequently present in lactobacillus strains isolated from patients with endocarditis, although it was able to express protein ca ... | 2004 | 15748979 |
| effect of probiotics on preventing disruption of the intestinal microflora following antibiotic therapy: a double-blind, placebo-controlled pilot study. | in this pilot-scale, double-blind, placebo-controlled trial, 30 patients with helicobacter pylori infection were randomised into three groups prior to their 7 days eradication therapy, to study the effects of probiotic supplement comprising lactobacillus acidophilus and bifidobacterium bifidum on the intestinal microflora in response to antibiotic therapy. group i received the placebo product from day 1 to day 15, group ii received placebo from day 1 to day 7 and probiotics from day 8 to day 15 ... | 2005 | 15829425 |
| quantitative real-time pcr assays to identify and quantify fecal bifidobacterium species in infants receiving a prebiotic infant formula. | a healthy intestinal microbiota is considered to be important for priming of the infants' mucosal and systemic immunity. breast-fed infants typically have an intestinal microbiota dominated by different bifidobacterium species. it has been described that allergic infants have different levels of specific bifidobacterium species than healthy infants. for the accurate quantification of bifidobacterium adolescentis, bifidobacterium angulatum, bifidobacterium bifidum, bifidobacterium breve, bifidoba ... | 2005 | 15870317 |
| novel putative galactose operon involving lacto-n-biose phosphorylase in bifidobacterium longum. | a lacto-n-biose phosphorylase (lnbp) was purified from the cell extract of bifidobacterium bifidum. its n-terminal and internal amino acid sequences were homologous with those of the hypothetical protein of bifidobacterium longum ncc2705 encoded by the bl1641 gene. the homologous gene of the type strain b. longum jcm1217, lnpa, was expressed in escherichia coli to confirm that it encoded lnbp. no significant identity was found with any proteins with known function, indicating that lnbp should be ... | 2005 | 15933016 |
| molecular properties and prebiotic effect of inulin obtained from artichoke (cynara scolymus l.). | a high molecular weight inulin has been prepared from artichoke (cynara scolymus l.) agroindustrial wastes using environmentally benign aqueous extraction procedures. physico-chemical analysis of the properties of artichoke inulin was carried out. its average degree of polymerization was 46, which is higher than for jerusalem artichoke, chicory, and dahlia inulins. gc-ms confirmed that the main constituent monosaccharide in artichoke inulin was fructose and its degradation by inulinase indicated ... | 2005 | 15960982 |
| oral probiotic bacterial administration suppressed allergic responses in an ovalbumin-induced allergy mouse model. | this study investigated whether orally administered probiotic bacteria (bifidobacterium bifidum and lactobacillus casei) and a gram-negative bacterium (escherichia coli) function as allergic immune modulators to prevent food allergy, according to the hygiene hypothesis. c3h/hej mice were sensitized with ovalbumin (ova) and cholera toxin for 5 weeks. after sensitization, the ova-induced mice that were not treated with bacteria had significantly increased levels of ova-specific ige, total ige, and ... | 2005 | 15963706 |
| evaluation of five probiotic products for label claims by dna extraction and polymerase chain reaction analysis. | label claims were evaluated for five probiotic products. specific oligonucleotide primers were designed for 11 species from the bifidobacterium, lactobacillus, and streptococcus genera. polymerase chain reaction, gel electrophoresis, and amplicon excision with dna sequencing were performed: sequence analysis and dna homology comparisons followed. bifidobacterium bifidum was not detected in two of the five samples by pcr analysis. also, lactobacillus species were found in two of the five product ... | 2005 | 15986864 |
| a novel galactooligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo. | prebiotics are nondigestible food ingredients that encourage proliferation of selected groups of the colonic microflora, thereby altering the composition toward a more beneficial community. in the present study, the prebiotic potential of a novel galactooligosaccharide (gos) mixture, produced by the activity of galactosyltransferases from bifidobacterium bifidum 41171 on lactose, was assessed in vitro and in a parallel continuous randomized pig trial. in situ fluorescent hybridization with 16s r ... | 2005 | 15987856 |
| culture-dependent and culture-independent qualitative analysis of probiotic products claimed to contain bifidobacteria. | a total of 58 probiotic products obtained worldwide, which were claimed to contain bifidobacterium strains (including 22 yoghurts, 5 dairy fruit drinks, 28 food supplements and 3 pharmaceutical preparations) were investigated in parallel using a culture-dependent and a culture-independent approach. three isolation media previously reported as selective for bifidobacterium were evaluated for their suitability in the quality analysis of these products. subsequently, possible bifidobacterial coloni ... | 2005 | 15992621 |
| ferrite supports for isolation of dna from complex samples and polymerase chain reaction amplification. | the influence of cobalt ferrite particles, with non-modified or modified surface, on the course of polymerase chain reaction (pcr) was investigated. dna isolated from bacterial cells of bifidobacterium bifidum was used in pcr evaluation of magnetic microspheres. the presence of cobalt ferrite particles inhibits pcr amplification. the effect is not dependent on the functional groups of the modifying reagents used (none, amino, carboxyl). amplification was improved after the magnetic separation of ... | 2005 | 16013619 |
| oral administration of bifidobacterium bifidum g9-1 suppresses total and antigen specific immunoglobulin e production in mice. | recent studies have suggested that oral bacteriotherapy with probiotics might be useful in the management of allergic diseases. we investigated the effect of oral administration of bifidobacterium bifidum g9-1 (bbg9-1) on immunoglobulin (ig) e production in balb/c mice. live bbg9-1 was orally administered to mice for 2 weeks from 1 week before ovalbumin (ova)-immunization. the treatment of bbg9-1 significantly reduced serum total ige level. in addition, bbg9-1 significantly and largely reduced t ... | 2005 | 16079493 |
| bifidobacterial lipoglycan as a new cause for false-positive platelia aspergillus enzyme-linked immunosorbent assay reactivity. | we previously hypothesized that a lipoglycan of bifidobacterium bifidum subsp. pennsylvanicum cross-reacts with the platelia aspergillus (pa) enzyme-linked immunosorbent assay (elisa) based on the presence of galactofuranosyl epitopes in the cell wall (m. a. s. h. mennink-kersten, r. r. klont, a. warris, h. j. m. op den camp, and p. e. verweij, lancet 363:325-327, 2004). we tested this hypothesis by testing bacterial suspensions of different bifidobacterial species and other gram-positive and -n ... | 2005 | 16081932 |
| prevotella massiliensis sp. nov. isolated from human blood. | we report a bacterial isolate (marseille isolate) recovered from the blood of a patient hospitalized in an intensive care unit, presenting with severe trauma, fever and mechanical ventilation. colonies appeared at 37 degrees c on blood agar after 72 h incubation. this isolate was a strictly anaerobic, gram-negative rod phenotypically related to other prevotella species described to date: non-motile, catalase-negative, oxidase-positive, non-glucose fermenting, resistant to vancomycin and suscepti ... | 2005 | 16085394 |
| study of adhesion and survival of lactobacilli and bifidobacteria on table olives with the aim of formulating a new probiotic food. | with the aim of developing new functional foods, a traditional product, the table olive, was used as a vehicle for incorporating probiotic bacterial species. survival on table olives of lactobacillus rhamnosus (three strains), lactobacillus paracasei (two strains), bifidobacterium bifidum (one strain), and bifidobacterium longum (one strain) at room temperature was investigated. the results obtained using a selected olive sample demonstrated that bifidobacteria and one strain of l. rhamnosus (la ... | 2005 | 16085808 |
| genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16s rrna gene analysis. | a novel approach combining a flow cytometric in situ viability assay with 16s rrna gene analysis was used to study the relationship between diversity and activity of the fecal microbiota. simultaneous staining with propidium iodide (pi) and syto bc provided clear discrimination between intact cells (49%), injured or damaged cells (19%), and dead cells (32%). the three subpopulations were sorted and characterized by denaturing gradient gel electrophoresis (dgge) of 16s rrna gene amplicons obtaine ... | 2005 | 16085863 |
| effect of probiotic supplementation on bacterial translocation in thermal injury. | to examine the effects of probiotic supplementation and enteral solutions containing glutamine and arginine on bacterial translocation (bt) and intestinal villous atrophy in thermal injury. | 2005 | 16133671 |
| production and characterization of anti-bifidobacteria monoclonal antibodies and their application in the development of an immuno-culture detection method. | an immuno-culture method has been developed by combination of specific monoclonal antibodies and plate culture to allow detection of viable bifidobacteria. cell wall proteins were selected as surface antigen to produce antibodies against bifidobacteria. the cell wall proteins were extracted and purified from six atcc strains of bifidobacteria grown in mrs broth using an anaerobic system. to compare the profile of the protein extracts, all the protein solutions obtained were analyzed by sds-page. ... | 2006 | 16182395 |
| timing of bifidobacterium administration influences the development of allergy to ovalbumin in mice. | c3h/hej mice were sensitized with ovalbumin (ova) and choleratoxin (ct) for 5 weeks, and then bifidobacterium bifidum bgn4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. after sensitization, the ova-induced (sham group) mice showed growth inhibition and had scab-covered tails which was associated with serum levels of 9887+/-175 ng ova-specific ige/ml and 758+/-525 ng igg1/ml. the sera of ... | 2005 | 16215850 |
| novel bifidobacterial glycosidases acting on sugar chains of mucin glycoproteins. | bifidobacterium bifidum was found to produce a specific 1,2-alpha-l-fucosidase. its gene (afc a) has been cloned and the dna sequence was determined. the afc a protein consisting of 1959 amino acid residues with a predicted molecular mass of 205 kda can be divided into three domains; the n-terminal function-unknown domain (576 aa), the catalytic domain (898 aa), and the c-terminal bacterial ig-like domain (485 aa). the recombinant catalytic domain specifically hydrolyzed the terminal alpha-(1--> ... | 2005 | 16233817 |
| conditions affecting cell surface properties of human intestinal bifidobacteria. | the cell surface properties of human intestinal bifidobacteria have been characterized for 30 strains isolated from a fecal sample. strain identification to the species level was obtained by restriction analysis of the amplified 16s rrna gene and confirmed by dna/dna reassociation experiments. the isolates were grouped in four genetically homogeneous clusters whose members belonged to bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium longum and bifidobacterium pseudocatenula ... | 2005 | 16284927 |
| suitability of bifidobacteria and thymol as biopreservatives in extending the shelf life of fresh packed plaice fillets. | the present study was aimed to evaluate the microbiological and sensory characteristics of biopreserved packed fresh plaice (pleuronectes platessa), during storage. | 2005 | 16313401 |
| modulation of antibody-mediated immune response by probiotics in chickens. | probiotic bacteria, including lactobacillus acidophilus and bifidobacterium bifidum, have been shown to enhance antibody responses in mammals. the objective of this study was to examine the effects of a probiotic product containing the above bacteria in addition to streptococcus faecalis on the induction of the chicken antibody response to various antigens, both systemically and in the gut. the birds received probiotics via oral gavage and subsequently were immunized with sheep red blood cells ( ... | 2005 | 16339061 |
| [analysis of bifidobacteria spp. composition in human gut via temperature gradient gel electrophoresis (tgge)]. | temperature gradient gel electrophoresis (tgge) method combined with 16s rdna clone library profiling was used to analyze bifidobacteria spp. composition in human gut in this study. bifidobacteria group-specific tgge patterns of 10 healthy human individuals showed that the bifidobacteria population in humans was host-specific. the genomic diversity and species composition of bifidobacteria in different individual was dissimilar. through sequencing and tgge comigration analysis of the bifidobacte ... | 2005 | 16342767 |
| determination of survival, identity and stress resistance of probiotic bifidobacteria in bio-yoghurts. | to determine the level of bifidobacteria in bio-yoghurts in the uk, identify the species, and compare the resistance of common bifidobacterium spp. to acidity and oxidative stress. | 2006 | 16478503 |
| antimicrobial susceptibility of bifidobacterium strains from humans, animals and probiotic products. | the aim of this study was to assess the antimicrobial susceptibility of a taxonomically diverse set of bifidobacterium strains to different classes of antimicrobial agents using a recently described medium. | 2006 | 16698847 |
| feruloyl oligosaccharides stimulate the growth of bifidobacterium bifidum. | insoluble dietary fiber from wheat bran contains some feruloyl groups linked to the arabinose residues in the cell wall arabinoxylan. treatment of wheat bran insoluble dietary fiber with xylanase from bacillus subtilis yielded feruloyl oligosacchairdes, which were purified with amberlite xad-2. saponification of the feruloyl oligosaccharides released ferulic acid and arabinoxylan oligosaccharides which consist of arabinose and xylose. the effect of the feruloyl oligosacchairdes on the growth of ... | 2005 | 16701572 |
| interaction of bifidobacteria with caco-2 cells-adhesion and impact on expression profiles. | the aim of the present study was to study different strains of bifidobacteria for adhesion to caco-2 intestinal epithelial cells (iecs) and to test for the mrna response of these cells following interaction with bifidobacteria. adhesion was tested at different ph conditions using model epithelia consisting of transwell cultures of fully differentiated caco-2 cells. microarrays were used to characterize changes in global expression profiles of caco-2 cells co-cultured with peripheral blood mononu ... | 2006 | 16713644 |
| comparison of three test media for antimicrobial susceptibility testing of bifidobacteria using the etest method. | the performance of three test media for antimicrobial susceptibility testing of bifidobacteria using the etest was compared. all bifidobacterium strains (n=42) displayed good growth on trypticase-phytone-yeast extract agar (tpy). most strains showed good growth on lactic acid bacteria susceptibility test medium supplemented with cysteine (lsm+cys); bifidobacterium bifidum showed moderate growth. growth of seven strains was inadequate on brucella blood agar (bru) and an additional eight strains s ... | 2006 | 16757151 |
| comparison of the concentrations of phenolic compounds in olive oils and other plant oils: correlation with antimicrobial activity. | the antimicrobial activity of different edible vegetable oils was studied. in vitro results revealed that the oils from olive fruits had a strong bactericidal action against a broad spectrum of microorganisms, this effect being higher in general against gram-positive than gram-negative bacteria. thus, olive oils showed bactericidal activity not only against harmful bacteria of the intestinal microbiota (clostridium perfringens and escherichia coli) also against beneficial microorganisms such as ... | 2006 | 16819902 |
| competitive exclusion of enteropathogens from human intestinal mucus by bifidobacterium strains with acquired resistance to bile--a preliminary study. | the ability to inhibit the adhesion and to displace selected pathogens from human intestinal mucus of two bifidobacterium strains with acquired resistance to bile, were assessed and compared with those of their bile sensitive original strains. a preliminary characterization of the macromolecules involved in the adhesion was also carried out. the inhibition of adhesion and the displacement of enteropathogens previously adhered were found to be specific, depending on the strains used. the cholate- ... | 2007 | 16842877 |
| pharmaceutical probiotics for the treatment of anaerobic and other infections. | pharmaceutical probiotics have been used as alternative treatments or preventative therapies for a variety of clinical diseases. the overuse of antibiotics and emergence of multiple-antibiotic resistant pathogens has refocused clinical attention on the field of probiotics. anaerobic infections which seem to respond well to probiotics are infections which involve the disruption of normal microbial flora. gastrointestinal infections (travelers' diarrhea, antibiotic-associated diarrhea,clostridium ... | 1997 | 16887565 |
| the intestinal microflora during the first weeks of life. | bacterial colonization of the neonatal gastrointestinal tract begins during birth when the neonate comes into contact with the maternal cervical and vaginal flora. in infants delivered by caesarean section, bacteria colonizing the neonate gastrointestinal tract are provided by the environment. the first bacteria encountered in the majority of healthy infants, are facultative anaerobes, which remain predominant during the first 2 weeks of life. among them, staphylococcus, enterobacteriaceae and s ... | 1997 | 16887585 |
| mixed culture fermentation studies on the effects of synbiotics on the human intestinal pathogens campylobacter jejuni and escherichia coli. | batch and continuous culture anaerobic fermentation systems, inoculated with human faeces, were utilised to investigate the antimicrobial actions of two probiotics, lactobacillus plantarum 0407, combined with oligofructose and bifidobacterium bifidum bb12, combined with a mixture of oligofructose and xylo-oligosaccharides (50:50 w/w) against e. coli and campylobacter jejuni. in batch fermenters, both e. coli and c. jejuni were inhibited by the synbiotics, even when the culture ph was maintained ... | 2003 | 16887709 |
| population dynamics of bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry. | the population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (fish) coupled with flow cytometry (fcm) analysis. although double-staining fish-fcm using both fluorescein isothiocyanate (fitc) and indodicarbocyanine (cy5) as labeling dyes for fecal samples has been reported, the analysis was interfered with by strong autofluorescence at the fitc fluorescence region because of the presence ... | 2006 | 17056700 |
| inhibitory impact of bifidobacteria on the transfer of beta-lactam resistance among enterobacteriaceae in the gnotobiotic mouse digestive tract. | while looking for new means to limit the dissemination of antibiotic resistance, we evaluated the role of potentially probiotic bifidobacteria on the transfer of resistance genes between enterobacteria. transfers of bla genes encoding extended-spectrum beta-lactamases (shv-5 and ctx-m-15) were studied in the absence or presence of bifidobacteria. in vitro, transfer frequencies of these bla genes decreased significantly in the presence of three of five tested strains, i.e., bifidobacterium longum ... | 2007 | 17122392 |
| high concentration cultivation of bifidobacterium bifidum in a submerged membrane bioreactor. | in batch cultures, after 25 h, the maximum cell mass of bifidobacterium bifidum bgn4 was 4.5 g/l, and the maximum cell count was 3.0 x 10(9) cfu/ml at ph 6.0 and 50 g/l sucrose. to increase the viable counts of bifidobacteria, cell retentive culture was applied using a submerged membrane bioreactor with suction and gas sparging. the maximum mass, count, and productivity of the cells after 36 h were 12.0 g/l, 2.2 x 10(10) cfu/ml, and 6.1 x 10(8) cfu/ml x h, respectively, at the feeding (dilution) ... | 2006 | 17137306 |
| sugar transport systems of bifidobacterium longum ncc2705. | here we present the complement of the carbohydrate uptake systems of the strictly anaerobic probiotic bifidobacterium longum ncc2705. the genome analysis of this bacterium predicts that it has 19 permeases for the uptake of diverse carbohydrates. the majority belongs to the atp-binding cassette transporter family with 13 systems identified. among them are permeases for lactose, maltose, raffinose, and fructooligosaccharides, a commonly used prebiotic additive. we found genes that encode a comple ... | 2007 | 17183207 |
| oral feeding of bifidobacterium bifidum (bgn4) prevents cd4(+) cd45rb(high) t cell-mediated inflammatory bowel disease by inhibition of disordered t cell activation. | probiotics have been considered as preventive agents for the control of inflammatory bowel disease (ibd). in this study, we assessed the immunomodulatory effect of bifidobacterium bifidum bgn4 on the control of ibd using the cd4(+) cd45rb(high) t cell transfer disease model. the mice were fed for 4 weeks with either a conventional diet containing only skim milk or a diet containing skim milk with 0.3% (w/w) bgn4. the bgn4-fed mice showed normal weight growth, fewer clinical symptoms such as thic ... | 2007 | 17218154 |
| [modulation of gastrointestinal physiology through probiotic strains of lactobacillus casei and bifidobacterium bifidum]. | the products called functional foods, which besides being merely nutritional have different beneficial effects on the organism, are situated in the context of diet and health promotion. amongst these functional foods we can distinguish, amongst others, between probiotic and prebiotic compounds. the micro-organisms most widely used in probiotic foods belong to the lactobacillus and bifidobacterium types. in this article we have studied the effect of diets supplemented with lactobacillus casei or ... | 2006 | 17224936 |
| combined effect of different lactic acid bacteria strains on the mode of cytokines pattern expression in human peripheral blood mononuclear cells. | the balance between immunogenic and tolerogenic activities in human immune system strongly depends on microflora-induced pro-and anti-inflammatory activities. lactic acid bacteria (lab) are important components of microflora. the interactions of the different strains of lab and the cells of immune system are largely unknown. to assess if lab strains composition would have an effect on the cellular responses profile (proliferation, cytokines synthesis) peripheral blood mononuclear cells (pbmc) mo ... | 2006 | 17242484 |
| selective colonization of insoluble substrates by human faecal bacteria. | insoluble plant polysaccharides and endogenous mucin are important energy sources for human colonic microorganisms. the object of this study was to determine whether or not specific communities colonize these substrates. using faecal samples from four individuals as inocula for an anaerobic in vitro continuous flow system, the colonization of wheat bran, high amylose starch and porcine gastric mucin was examined. recovered substrates were extensively washed and the remaining tightly attached bac ... | 2007 | 17298367 |
| meta-analysis of probiotics for the prevention of traveler's diarrhea. | traveler's diarrhea (td) is a common health complaint among travelers. rates of td can range from 5% to 50%, depending on the destination. the use of probiotics for this disease remains controversial. the objective of this study was to compare the efficacy of probiotics for the prevention of td based on published randomized, controlled clinical trials. | 2007 | 17298915 |
| quantitative assessment of faecal bifidobacterial populations by real-time pcr using lanthanide probes. | to develop real-time quantitative pcr methods, based on the use of probes labelled with a stable fluorescent lanthanide chelate, for the quantification of different human faecal bifidobacterial populations. | 2007 | 17381755 |
| basic characteristics of sporolactobacillus inulinus bcrc 14647 for potential probiotic properties. | the basic characteristics of the spore-forming lactic acid bacterium, sporolactobacillus inulinus bcrc 14647, was evaluated in vitro for its potential probiotic properties. assessments including acid and bile salt tolerance, adhesiveness, and antagonistic effects on pathogenic salmonella enteritidis bcrc 10744, as well as inhibition factors of spent culture supernatant (scs) and an invasion assay, were conducted using lactobacillus acidophilus bcrc 10695 and two bifidobacteria (bifidobacterium b ... | 2007 | 17387552 |