| depolymerisation and biodegradation of a synthetic tanning agent by activated sludges, the bacteria arthrobacter globiformis and comamonas testosteroni, and the fungus cunninghamella polymorpha. | degradation of a synthetic tanning agent cnsf (a condensation product of 2-naphthalenesulfonic acid (2-nsa) and formaldehyde) by four activated sludges, two previously characterised bacterial strains, arthrobacter sp. 2ac and comamonas sp. 4bc, and the fungus cunninghamella polymorpha, was studied in batch culture at 25 degrees c by determining the changes in the concentrations of cnsf and its component monomers and oligomers (n2-n11). the loss of individual oligomers was correlated with the len ... | 2005 | 15865336 |
| an enzymatically produced novel cyclic tetrasaccharide, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->} (cyclic maltosyl-(1-->6)-maltose), from starch. | a bacterial strain m6, isolated from soil and identified as arthrobacter globiformis, produced a novel nonreducing oligosaccharide. the nonreducing oligosaccharide was produced from starch using a culture supernatant of the strain as enzyme preparation. the oligosaccharide was purified as a crystal preparation after alkaline treatment and deionization of the reaction mixture. the structure of the oligosaccharide was determined by methylation analysis, mass spectrometry, and (1)h and (13)c nmr sp ... | 2005 | 15882856 |
| crystal structure of dmgo provides a prototype for a new tetrahydrofolate-binding fold. | the crystal structure of dmgo (dimethylglycine oxidase) from arthrobacter globiformis in complex with folate compounds has revealed a novel thf (tetrahydrofolate)-binding fold [leys, basran and scrutton (2003) embo j. 22, 4038-4048]. this fold is widespread among folate-binding proteins. the crystal structures of aminomethyltransferase (t-protein), ygfz and trme all reveal similar thf-binding folds despite little similarity in sequence or function. the thf-binding site is highly specific for red ... | 2005 | 16042597 |
| reinvestigation of metal ion specificity for quinone cofactor biogenesis in bacterial copper amine oxidase. | the topa quinone (tpq) cofactor of copper amine oxidase is generated by copper-assisted self-processing of the precursor protein. metal ion specificity for tpq biogenesis has been reinvestigated with the recombinant phenylethylamine oxidase from arthrobacter globiformis. besides cu2+ ion, some divalent metal ions such as co2+, ni2+, and zn2+ were also bound to the metal site of the apoenzyme so tightly that they were not replaced by excess cu2+ ions added subsequently. although these noncupric m ... | 2005 | 16142901 |
| reversible inhibition of copper amine oxidase activity by channel-blocking ruthenium(ii) and rhenium(i) molecular wires. | molecular wires comprising a ru(ii)- or re(i)-complex head group, an aromatic tail group, and an alkane linker reversibly inhibit the activity of the copper amine oxidase from arthrobacter globiformis (agao), with k(i) values between 6 mum and 37 nm. in the crystal structure of a ru(ii)-wire:agao conjugate, the wire occupies the agao active-site substrate access channel, the trihydroxyphenylalanine quinone cofactor is ordered in the "off-cu" position with its reactive carbonyl oriented toward th ... | 2005 | 16157884 |
| the role of histidine 200 in mndd, the mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase from arthrobacter globiformis cm-2, a site-directed mutagenesis study. | the manganese-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) from arthrobacter globiformis cm-2 is an extradiol-cleaving catechol dioxygenase that catalyzes aromatic ring cleavage of 3,4-dihydroxyphenylacetate (dhpa). based on the recent crystal structure of the mndd-dhpa complex, a series of site-directed mutations were made at a conserved second-sphere residue, histidine 200, to gain insight into and clarify the role this residue plays in the mn(ii)-dependent catalytic mechanism. ... | 2005 | 16217642 |
| isolation and characterization of psychrotrophs from subterranean environments. | subterranean environments are potential sources for the isolation of novel microorganisms. water and soil samples were collected at depths ranging from 10 to 1800 meters below the surface, and screening was carried out with aerobic rich and anaerobic minimal media. two psychrotrophic and three chemoautotrophic strains were isolated. one of the psychrotrophic isolates, designated sn16a, grew at temperatures between -5 and 37 degrees c with optimal growth between 25 and 30 degrees c. the other psy ... | 1999 | 16232548 |
| cloning of inulin fructotransferase (dfa iii-producing) gene from arthrobacter globiformis c11-1. | a gene encoding an inulin fructotransferase (dfa iii-producing) [ec 2.4.1.93] from arthrobacter globiformis c11-1 was cloned and the nucleotide sequence was determined. the cloned fragment contained a 1353 bp open reading frame. the initiation codon was estimated to be an unusual codon, gtg. the gene encoded a signal peptide (40 amino acid residues) for secretion. the molecular mass of the native enzyme was calculated as 43,400 da from the sequencing data. the deduced amino acid sequence of the ... | 2000 | 16232803 |
| purification and properties of betaine aldehyde dehydrogenase with high affinity for nadp from arthrobacter globiformis. | betaine aldehyde dehydrogenase from arthrobacter globiformis was purified to apparent homogeneity by ammonium sulfate fractionation, followed by ion-exchange, butyl-toyopearl and gel filtration chromatography. the enzyme was found to be a tetramer with identical 55 kda subunits. both nad+ and nadp+ could be used as a cofactor for the enzyme and michaelis constants (k(m) value) for nad+ and nadp+ were 1075 microm and 48 microm, respectively. the enzyme was highly specific for betaine aldehyde and ... | 2002 | 16233177 |
| molecular cloning of the gene encoding the di-d-fructofuranose 1,2':2,3' dianhydride hydrolysis enzyme (dfa iiiase) from arthrobacter sp. h65-7. | the gene encoding an intracellular enzyme hydrolyzing di-d-fructofuranose 1,2':2,3' dianhydride (dfa iii) (dfa iiiase) was cloned from the genomic dna of arthrobacter sp. h65-7 for the first time. the single open reading frame (orf) of the dfa iiiase gene consisted of 1368-bp encoding 455 amino acids. dfa iiiase showed a phylogenetically distinct position from other inulin-degrading enzymes and showed similarity only with inulin fructotransferases (depolymerizing) (inulase ii, ec 2.4.1.93) from ... | 2003 | 16233453 |
| a thermostable histamine oxidase from arthrobacter crystallopoietes kait-b-007. | a thermostable histamine oxidase (ec 1.4.3.-) was found in cells of arthrobacter crystallopoietes kait-b-007 isolated from soil. the enzyme was purified about 715-fold over the cell free extracts with a yield of 55% by ammonium sulfate fractionation and various column chromatographies. the purified enzyme was homogeneous on polyacrylamide gel-electrophoresis (native-page). when the enzyme was kept at 65 degrees c and 70 degrees c for 10 min, the activity was fully stable at 65 degrees c and decr ... | 2004 | 16233600 |
| cloning and heterologous expression of a glucodextranase gene from arthrobacter globiformis i42, and experimental evidence for the catalytic diad of the recombinant enzyme. | the gene encoding a glucodextranase from arthrobacter globiformis i42 was cloned and, subsequently, heterologously expressed in escherichia coli. this glucodextranase gene consists of 1048 amino acid residues with a calculated molecular mass of 109,135 da. the roles of two residues at the active site of a. globiformis i42 glucodextranase were examined by site-directed mutagenesis. glutamic acid residues 458 and 656, which are part of the apparent catalytic residues, were found to be essential fo ... | 2004 | 16233603 |
| cloning and heterologous expression of a beta-fructofuranosidase gene from arthrobacter globiformis ifo 3062, and site-directed mutagenesis of the essential aspartic acid and glutamic acid of the active site. | we have cloned the gene encoding a beta-fructofuranosidase from arthrobacter globiformis ifo 3062, and subsequently, the gene was heterologously expressed in escherichia coli. this beta-fructofuranosidase gene encodes a protein of 548 amino acid residues with a calculated molecular mass of 60,519 da. we have examined the roles of three residues of a. globiformis ifo 3062 beta-fructofuranosidase by site-directed mutagenesis, and found that aspartic acid 130 and glutamic acid 392, which are two of ... | 2004 | 16233623 |
| molecular cloning and expression of uricase gene from arthrobacter globiformis in escherichia coli and characterization of the gene product. | arthrobacter globiformis ferm bp-360 produces uricase (urate oxidase; ec 1.7.3.3) intracellularly. a genomic library of the bacterium, prepared in the plasmid vector puc118, was screened with probes based on the amino acid sequence of the purified uricase. we found that a chimeric plasmid in the library, designated puod1, carries a 2.0-kb dna insert from the arthrobacter dna that hybridizes with the probe. the dna insert contains an orf consisting of 302 amino acids with a calculated molecular m ... | 2004 | 16233683 |
| attachment to autoclaved soil of bacterial cells from pure cultures of soil isolates. | pure cultures of arthrobacter globiformis and four fresh soil isolates were incubated individually in autoclaved soil, in both the presence and absence of glucose. these bacteria grew in the soil and, except for a. globiformis, eventually attached firmly to the soil solids. firmly attached cells were defined as those which could not be separated from the soil solids by blending combined with a series of low-speed centrifugal washings. the attachment attained by the soil isolates appeared to dupl ... | 1979 | 16345373 |
| production of d-tagatose from dulcitol by arthrobacter globiformis. | a process for the bacterial oxidation of dulcitol to d-tagatose has been developed. the strain arthrobacter globiformis st48 used in this fermentation was isolated from soil. the yield of d-tagatose accumulated in the medium from dulcitol was as high as 85%. about 14 g of d-tagatose crystals was isolated from 1 liter of 2% dulcitol medium. | 1984 | 16346663 |
| identification of eubacteria isolated from leaf cavities of four species of the n-fixing azolla fern as arthrobacter conn and dimmick. | the nutritional and physiological characteristics of 15 isolates from four species of the azolla fern were determined. although some minor variation existed in levels of urease activity, ability to utilize xylose, and formation of n(2) gas from no(3), all 15 isolates were rather similar and believed to represent a single species. these eubacteria exhibited aminopeptidase activity and became viscous when treated with koh, similar to gram-negative organisms; however, the absence of lipopolysacchar ... | 1986 | 16347144 |
| protozoan response to the addition of bacterial predators and other bacteria to soil. | representatives of several categories of bacteria were added to soil to determine which of them might elicit responses from the soil protozoa. the various categories were nonobligate bacterial predators of bacteria, prey bacteria for these predators, indigenous bacteria that are normally present in high numbers in soil, and non-native bacteria that often find their way in large numbers into soil. the soil was incubated and the responses of the indigenous protozoa were determined by most-probable ... | 1989 | 16347983 |
| competition for ammonium between nitrifying and heterotrophic bacteria in dual energy-limited chemostats. | the absence of nitrification in soils rich in organic matter has often been reported. therefore, competition for limiting amounts of ammonium between the chemolithotrophic ammonium-oxidizing species nitrosomonas europaea and the heterotrophic species arthrobacter globiformis was studied in the presence of nitrobacter winogradskyi in continuous cultures at dilution rates of 0.004 and 0.01 h. ammonium limitation of a. globiformis was achieved by increasing the glucose concentration in the reservoi ... | 1991 | 16348588 |
| effects of grazing by flagellates on competition for ammonium between nitrifying and heterotrophic bacteria in chemostats. | the enhanced mineralization of organic nitrogen by bacteriophagous protozoa is thought to favor the nitrification process in soils, in which nitrifying bacteria have to compete with heterotrophic bacteria for the available ammonium. to obtain more insight into this process, the influence of grazing by the bacteriovorous flagellate adriamonas peritocrescens on the competition for limiting amounts of ammonium between the ammonium-oxidizing species nitrosomonas europaea and the heterotrophic specie ... | 1992 | 16348722 |
| competition for ammonium between nitrifying and heterotrophic bacteria in continuously percolated soil columns. | although the absence of nitrate formation in grassland soils rich in organic matter has often been reported, low numbers of nitrifying bacteria are still found in these soils. to obtain more insight into these observations, we studied the competition for limiting amounts of ammonium between the chemolithotrophic ammonium-oxidizing species nitrosomonas europaea and the heterotrophic species arthrobacter globiformis in the presence of nitrobacter winogradskyi with soil columns containing calcareou ... | 1992 | 16348787 |
| effects of grazing by flagellates on competition for ammonium between nitrifying and heterotrophic bacteria in soil columns. | the enhanced mineralization of immobilized nitrogen by bacteriophagous protozoa has been thought to favor the nitrification process in soils in which nitrifying bacteria must compete with heterotrophic bacteria for the available ammonium. to obtain more insight into this process, the influence of grazing by the flagellate adriamonas peritocrescens on the competition for ammonium between the chemolithotrophic species nitrosomonas europaea and the heterotrophic species arthrobacter globiformis in ... | 1993 | 16348988 |
| arthrobacter globiformis and its bacteriophage in soil. | bacteriophages in soil for arthrobacter globiformis were rarely detected unless the soil was nutritionally amended and incubated. in amended soil, phage were continuously produced for at least 48 h, and this did not require the addition of host cells. rod and spheroid stage host cells added to the amended soil encountered indigenous bacteriophage, but added phage did not encounter sensitive indigenous host cells for some time, if at all. the indigenous phage in nonincubated soil seemed to be pre ... | 1974 | 16350007 |
| electrochemical sensor based on arthrobacter globiformis for cholinesterase activity determination. | the sensors applied recently for determination of cholinesterase activity are mostly enzymatic amperometric sensors, in spite of their disadvantages: short life-time at ambient temperature, instability of the response, interferences, as well as passivation of the electrode surface. in the present paper a new approach for determination of cholinesterase activity was proposed, overcoming the main drawbacks of the analysis performed with amperometric enzymatic sensors. instead of the immobilization ... | 2006 | 16376069 |
| decolorization and degradation of methylene blue by arthrobacter globiformis. | | 2005 | 16402302 |
| treatment of naphthalene-2-sulfonic acid from tannery wastewater by a granular activated carbon fixed bed inoculated with bacterial isolates arthrobacter globiformis and comamonas testosteroni. | the kinetics of naphthalene-2-sulfonic acid (2-nsa) adsorption by granular activated carbon (gac) were measured and the relationships between adsorption, desorption, bioavailability and biodegradation assessed. the conventional langmuir model fitted the experimental sorption isotherm data and introduced 2-nsa degrading bacteria, established on the surface of the gac, did not interfere with adsorption. the potential value of gac as a microbial support in the aerobic degradation of 2-nsa by arthro ... | 2006 | 16427119 |
| acaricomes phytoseiuli gen. nov., sp. nov., isolated from the predatory mite phytoseiulus persimilis. | a gram-positive, rod-shaped, non-spore-forming bacterium, strain csct, was isolated from diseased, surface-sterilized specimens of the predatory mite phytoseiulus persimilis athias-henriot and subjected to polyphasic taxonomic analysis. comparative analysis of the 16s rrna gene sequence revealed that the strain was a new member of the family micrococcaceae. nearest phylogenetic neighbours were determined as renibacterium salmoninarum (94.0%), arthrobacter globiformis (94.8%) and arthrobacter rus ... | 2006 | 16449459 |
| purification, characterization, and gene cloning of a novel maltosyltransferase from an arthrobacter globiformis strain that produces an alternating alpha-1,4- and alpha-1,6-cyclic tetrasaccharide from starch. | a glycosyltransferase, involved in the synthesis of cyclic maltosylmaltose [cmm; cyclo-{-->6)-alpha-d-glcp(1-->4)-alpha-d-glcp(1-->6)-alpha-d-glcp(1-->4)-alpha-d-glcp(1-->}] from starch, was purified to homogeneity from the culture supernatant of arthrobacter globiformis m6. the cmm-forming enzyme had a molecular mass of 71.7 kda and a pi of 3.6. the enzyme was most active at ph 6.0 and 50 degrees c and was stable from ph 5.0 to 9.0 and up to 30 degrees c. the addition of 1 mm ca2+ enhanced the ... | 2006 | 16461650 |
| allergenicity assessment of transgenic mustard (brassica juncea) expressing bacterial coda gene. | assessing the allergenicity and toxicity of genetically modified (gm) crops is essential before they become a regular part of our food supply. the present study aimed to assess the allergenicity of brassica juncea (mustard) expressing choline oxidase (coda) gene from arthrobacter globiformis that provides resistance against abiotic stresses. | 2006 | 16512812 |
| stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase: cloning, nucleotide sequence, and overexpression of the esterase gene of arthrobacter globiformis in escherichia coli. | volume 61, no. 9, p. 3209, column 2, line 23: "l22165" should read "l22516." [this corrects the article on p. 3208 in vol. 61.]. | 1996 | 16535302 |
| kinetic and structural studies on the catalytic role of the aspartic acid residue conserved in copper amine oxidase. | copper amine oxidase contains a post-translationally generated quinone cofactor, topa quinone (tpq), which mediates electron transfer from the amine substrate to molecular oxygen. the overall catalytic reaction is divided into the former reductive and the latter oxidative half-reactions based on the redox state of tpq. in the reductive half-reaction, substrate amine reacts with the c5 carbonyl group of the oxidized tpq, forming the substrate schiff base (tpq(ssb)), which is then converted to the ... | 2006 | 16566584 |
| a comparative study of the binding and inhibition of four copper-containing amine oxidases by azide: implications for the role of copper during the oxidative half-reaction. | copper amine oxidases (cuaos) catalyze the oxidative deamination of primary amines operating through a ping-pong bi-bi mechanism. in this work, azide (an exogenous monodentate ligand) was used to probe the role of copper during the oxidative half-reaction of cuao catalysis. the effects of azide on both the reductive and oxidative half-reactions of pea seedling amine oxidase (psao), the recombinant human kidney diamine oxidase (rhdao), arthrobacter globiformis amine oxidase (agao), and pichia pas ... | 2006 | 16846222 |
| intramolecular electron transfer rate between active-site copper and tpq in arthrobacter globiformis amine oxidase. | copper amine oxidases catalyze the oxidative deamination of primary amines operating through a ping-pong bi bi mechanism, divided into reductive and oxidative half-reactions. considerable debate still exists regarding the role of copper in the oxidative half-reaction, where o2 is reduced to h2o2. substrate-reduced amine oxidases display an equilibrium between a cu(ii) aminoquinol and a cu(i) semiquinone, with the magnitude of the equilibrium constant being dependent upon the enzyme source. the i ... | 2006 | 16924556 |
| mechanism of fad reduction and role of active site residues his-225 and tyr-259 in arthrobacter globiformis dimethylglycine oxidase: analysis of mutant structure and catalytic function. | residues his-225 and tyr-259 are located close to the fad in the dehydrogenase active site of the bifunctional dimethylglycine oxidase (dmgo) of arthrobacter globiformis. we have suggested [leys, d., basran, j., and scrutton, n. s. (2003) embo j. 22, 4038-4048] that these residues are involved in abstraction of a proton from the substrate amine group of dimethylglycine prior to c-h bond breakage and fad reduction. to investigate this proposal, we have isolated two mutant forms of dmgo in which ( ... | 2006 | 16964976 |
| functional analysis of the twin-arginine translocation pathway in corynebacterium glutamicum atcc 13869. | compared to those of other gram-positive bacteria, the genetic structure of the corynebacterium glutamicum tat system is unique in that it contains the tate gene in addition to tata, tatb, and tatc. the tate homologue has been detected only in the genomes of gram-negative enterobacteria. to assess the function of the c. glutamicum tat pathway, we cloned the tata, tatb, tatc, and tate genes from c. glutamicum atcc 13869 and constructed mutants carrying deletions of each tat gene or of both the ta ... | 2006 | 16997984 |
| ecotoxicological assessment of sediment, suspended matter and water samples in the upper danube river. a pilot study in search for the causes for the decline of fish catches. | goals, scope and background: fish populations, especially those of the grayling (thymallus thymallus), have declined over the last two decades in the upper danube river between sigmaringen and ulm, despite intensive and continuous stocking and improvement of water quality since the 1970s. similar problems have been reported for other rivers, e.g. in switzerland, great britain, the united states and canada. in order to assess if ecotoxicological effects might be related to the decline in fish cat ... | 2006 | 17067025 |
| the copper-containing amine oxidase from arthrobacter globiformis: refinement at 1.55 and 2.20 a resolution in two crystal forms. | copper-containing amine oxidases are found in all the major kingdoms of life. they catalyse the oxidation of organic amines in the presence of molecular dioxygen to aldehydes and hydrogen peroxide. the catalytic centres contain a cu atom and a topaquinone cofactor formed autocatalytically from a tyrosine residue in the presence of cu and molecular oxygen. the structure of the cu-containing amine oxidase from arthrobacter globiformis, which was previously refined at 1.8 a resolution in space grou ... | 2006 | 17077478 |
| the coda transgene for glycinebetaine synthesis increases the size of flowers and fruits in tomato. | the tolerance of various species of plant to abiotic stress has been enhanced by genetic engineering with certain genes. however, the use of such transgenes is often associated with negative effects on growth and productivity under non-stress conditions. the coda gene from arthrobacter globiformis is of particular interest with respect to the engineering of desirable productive traits in crop plants. the expression of this gene in tomato plants resulted in significantly enlarged flowers and frui ... | 2007 | 17362485 |
| [obtaining and characterization of dna-containing micromummies of yeasts and gram-positive bacteria with enhanced cell wall permeability: application in pcr]. | the procedure of obtaining dna-containing cell envelopes ("micromummies") of bacteria, yeasts, and fungi using chaotropic salts has been developed previously and the possibility of their direct application in pcr has been demonstrated. the fine structure of micromummies has been studied by electron microscopic methods. this work has demonstrated that additional treatment of micromummies of yeasts and gram-positive bacteria with proteinase k results in hydrolytic degradation of cell proteins and ... | 2007 | 17410877 |
| chlamydomonas reinhardtii, a model system for functional validation of abiotic stress responsive genes. | stress tolerance is a multigenic character and there are many stress responsive genes, which are stress specific. although many of these have been cloned, their functional significance remains fragmentary. hence it is important to identify the relevant stress genes involved in altering the metabolism for adaptation. overexpression is one of the several approaches and chlamydomonas is a suitable system to study the functional relevance of stress genes. stress responses can only be assessed on pri ... | 2007 | 17431668 |
| glycinebetaine accumulation is more effective in chloroplasts than in the cytosol for protecting transgenic tomato plants against abiotic stress. | tomato (lycopersicon esculentum mill. cv. moneymaker) plants were transformed with a gene for choline oxidase (coda) from arthrobacter globiformis. the gene product (coda) was targeted to the chloroplasts (chl-coda), cytosol (cyt-coda) or both compartments simultaneously (chlcyt-coda). these three transgenic plant types accumulated different amounts and proportions of glycinebetaine (gb) in their chloroplasts and cytosol. targeting coda to either the cytosol or both compartments simultaneously i ... | 2007 | 17617827 |
| the distribution of acetohydroxyacid synthase in soil bacteria. | most bacteria possess the enzyme acetohydroxyacid synthase, which is used to produce branched-chain amino acids. enteric bacteria contain several isozymes suited to different conditions, but the distribution of acetohydroxyacid synthase in soil bacteria is largely unknown. growth experiments confirmed that escherichia coli, salmonella enterica serotype typhimurium, and enterobacter aerogenes contain isozymes of acetohydroxyacid synthase, allowing the bacteria to grow in the presence of valine (w ... | 2008 | 17624809 |
| trapping of a dopaquinone intermediate in the tpq cofactor biogenesis in a copper-containing amine oxidase from arthrobacter globiformis. | the biogenesis of the topaquinone (tpq) cofactor of copper amine oxidase (cao) is self-catalyzed and requires copper and molecular oxygen. a dopaquinone intermediate has been proposed to undergo 1,4-addition of a copper-associated water molecule to form the reduced form of tpq (tpq(red)), followed by facile oxidation by o(2) to yield the mature tpq (tpq(ox)). in this study, we have incorporated a lysine residue in the active site of arthrobacter globiformis cao (agao) by site-directed mutagenesi ... | 2007 | 17715921 |
| cloning and characterization of a 4-hydroxyphenylacetate 3-hydroxylase from the thermophile geobacillus sp. pa-9. | a 4-hydroxyphenylacetic acid (4-hpa) hydroxylase-encoding gene, on a 2.7-kb genomic dna fragment, was cloned from the thermophile geobacillus sp. pa-9. the geobacillus sp. pa-9 4-hpa hydroxylase gene, designated hpah, encodes a protein of 494 amino acids with a predicted molecular mass of 56.269 da. the deduced amino-acid sequence of the hpah gene product displayed <30% amino-acid sequence identity with the larger monooxygenase components of the previously characterized two-component 4-hpa 3-hyd ... | 2007 | 17805928 |
| cloning, expression, and characterization of bacillus sp. snu-7 inulin fructotransferase. | a gene encoding inulin fructotransferase (di-d-fructofuranose 1,2': 2,3' dianhydride [dfa iii]-producing iftase, ec 4.2.2.18) from bacillus sp. snu-7 was cloned. this gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a 410-amino acid mature protein. the deduced amino acid sequence was 98% identical to arthrobacter globiformis c11-1 iftase (dfa iii-producing). the enzyme was successfully expressed in e. coli as a functiona ... | 2007 | 18051351 |
| [effect of quinocitrinines from the fungus penicillium citrinum on the respiration of yeasts and bacteria]. | we studied the effect of quinocitrinines on the respiratory activity of yeasts (yarrowia lipolytica) and bacteria (arthrobacter globiformis). quinocitrinines were shown to activate respiration of native cells in both types of organisms. studies of yeast mitochondria showed that quinocitrinine exerts an uncoupling effect on oxidative phosphorylation, which activates the respiration, reduces the respiratory control, and decreases the adp/o ratio. experiments with intact mitochondria and native cel ... | 2007 | 18173114 |
| rapid mineralization of the phenylurea herbicide diuron by variovorax sp. strain srs16 in pure culture and within a two-member consortium. | the phenylurea herbicide diuron [n-(3,4-dichlorophenyl)-n,n-dimethylurea] is widely used in a broad range of herbicide formulations, and consequently, it is frequently detected as a major water contaminant in areas where there is extensive use. we constructed a linuron [n-(3,4-dichlorophenyl)-n-methoxy-n-methylurea]- and diuron-mineralizing two-member consortium by combining the cooperative degradation capacities of the diuron-degrading organism arthrobacter globiformis strain d47 and the linuro ... | 2008 | 18296530 |
| swapping metals in fe- and mn-dependent dioxygenases: evidence for oxygen activation without a change in metal redox state. | biological o(2) activation often occurs after binding to a reduced metal [e.g., m(ii)] in an enzyme active site. subsequent m(ii)-to-o(2) electron transfer results in a reactive m(iii)-superoxo species. for the extradiol aromatic ring-cleaving dioxygenases, we have proposed a different model where an electron is transferred from substrate to o(2) via the m(ii) center to which they are both bound, thereby obviating the need for an integral change in metal redox state. this model is tested by usin ... | 2008 | 18492808 |
| enantiomer-specific binding of ruthenium(ii) molecular wires by the amine oxidase of arthrobacter globiformis. | the copper amine oxidase from arthrobacter globiformis (agao) is reversibly inhibited by molecular wires comprising a ru(ii) complex head group and an aromatic tail group joined by an alkane linker. the crystal structures of a series of ru(ii)-wire-agao complexes differing with respect to the length of the alkane linker have been determined. all wires lie in the agao active-site channel, with their aromatic tail group in contact with the trihydroxyphenylalanine quinone (tpq) cofactor of the enzy ... | 2008 | 18507382 |
| growth and phosphate uptake of a high phosphate accumulating bacterium, arthrobacter globiformis pab-6 in continuous culture. | a high phosphate accumulating bacterium, arthrobacter globiformis pab-6, was grown in a chemostat under glucose-limitation. two different growth patterns at steady state with various dilution rates were obtained. in one case, cells having a coccus shape tended to washout at a low dilution rate, 0.2 (h(-1)). in another, cells with a rod shape grew faster and gave a good steady-state growth at a dilution rate of 0.4. such a close relationship between growth rate and cell morphology was found both ... | 1983 | 18551481 |
| kinetic model of hydrocortisone 1-en-dehydrogenation by arthrobacter globiformis. | a kinetic model of the hydrocortisone-to-prednisolone transformation by arthrobacter globiformis is constructed using the experimental data obtained in studies of this process. besides adequately describing experimental data, the model allows one to determine the relation between hydrocortisone oxidation and the level of endogenous substrates in bacterial cells, and the relation between the saturating concentration of hydrocortisone in the enzymic system of bacteria and the content of endogenous ... | 1989 | 18587966 |
| kinetic model for the regulation of redox reaction in steroid transformation by arthrobacter globiformis cells. | a kinetic model of hydrocortisone transformation was developed in studies of the kinetics of biochemical systems. the regulatory bases of the model are the biosynthesis of steroid-transforming enzymes and their activity, the level of endogenous substrates, the respiratory chain activity, and the initial concentrations of reagents. when compared, the experimental data completely coincide with the results of the computer modeling, the coincidence being not only qualitative but also quantitative. i ... | 1989 | 18587967 |
| characterization and validation of a bioluminescent bioreporter for the direct detection of escherichia coli. | a two-component bacteriophage-based bioluminescent reporter system was developed for the detection of escherichia coli in environmental samples. the bioreporter system consists of a luxi integrated lambda bacteriophage and a lux-based bioluminescent reporter cell that responds to the infection event through acyl-homoserine lactone (ahl) mediated quorum sensing and bioluminescent signal stimulation. this work addresses the ability of the bioreporter system to detect and quantify the target pathog ... | 2008 | 18593593 |
| purification and characterization of cyclic maltosyl-(1-->6)-maltose hydrolase and alpha-glucosidase from an arthrobacter globiformis strain. | cyclic maltosyl-maltose [cmm, cyclo-[-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->]], a novel cyclic tetrasaccharide, has a unique structure. its four glucose residues are joined by alternate alpha-1,4 and alpha-1,6 linkages. cmm is synthesized from starch by the action of 6-alpha-maltosyltransferase from arthrobacter globiformis m6. recently, we determined the mechanism of extracellular synthesis of cmm, but the degrading pathway of the saccharide remai ... | 2008 | 18603794 |
| complexes of the copper-containing amine oxidase from arthrobacter globiformis with the inhibitors benzylhydrazine and tranylcypromine. | complexes of arthrobacter globiformis amine oxidase (agao) with the inhibitors benzylhydrazine and tranylcypromine (an antidepressant drug) have been refined at 1.86 and 1.65 a resolution, respectively. both inhibitors form covalent adducts with the tpq cofactor. a tyrosine residue, proposed to act as a gate to the agao active site, is in its open conformation. | 2008 | 18607080 |
| further insight into the mechanism of stereoselective proton abstraction by bacterial copper amine oxidase. | during the catalytic reaction of copper amine oxidase, one of the two prochiral hydrogen atoms at the c1 position of substrate amine is stereoselectively abstracted by a conserved asp residue serving as a general base. using stereospecifically deuterium-labeled enantiomers of 2-phenylethylamine, we previously showed that the pro-s alpha-proton is abstracted by the enzyme from arthrobacter globiformis (agao) [uchida, m., et al. (2003) biosci. biotechnol. biochem. 67, 2664-2667]. more recently, we ... | 2008 | 18627131 |
| structures of arthrobacter globiformis urate oxidase-ligand complexes. | the enzyme urate oxidase catalyzes the conversion of uric acid to 5-hydroxyisourate, one of the steps in the ureide pathway. arthrobacter globiformis urate oxidase (aguox) was crystallized and structures of crystals soaked in the substrate uric acid, the inhibitor 8-azaxanthin and allantoin have been determined at 1.9-2.2 a resolution. the biological unit is a homotetramer and two homotetramers comprise the asymmetric crystallographic unit. each subunit contains two t-fold domains of betabetaalp ... | 2008 | 18645230 |
| recovery of choline oxidase activity by in vitro recombination of individual segments. | initial attempts to express a choline oxidase from arthrobacter pascens (apcho-syn) in escherichia coli starting from a synthetic gene only led to inactive protein. however, activity was regained by the systematic exchange of individual segments of the gene with segments from a choline oxidase-encoding gene from arthrobacter globiformis yielding a functional chimeric enzyme. next, a sequence alignment of the exchanged segment with other choline oxidases revealed a mutation in the apcho-syn, show ... | 2008 | 18704397 |
| safety assessment of bacterial choline oxidase protein introduced in transgenic crops for tolerance against abiotic stress. | genetically modified crops have resistance to abiotic stress by introduction of choline oxidase protein. in the present study, the safety of choline oxidase protein derived from arthrobacter globiformis was assessed for toxicity and allergenicity. the protein was stable at 90 degrees c for 1 h. toxicity studies of choline oxidase in mice showed no significant difference (p > 0.05) from control in terms of growth, body weight, food consumption, and blood biochemical indices. histology of gut tiss ... | 2008 | 19035641 |
| kinetics and spectroscopic evidence that the cu(i)-semiquinone intermediate reduces molecular oxygen in the oxidative half-reaction of arthrobacter globiformis amine oxidase. | the role of copper during the reoxidation of substrate-reduced amine oxidases by o(2) has not yet been definitively established. both outer-sphere and inner-sphere pathways for the reduction of o(2) to h(2)o(2) have been proposed. a key step in the inner-sphere mechanism is the reaction of o(2) directly with the cu(i) center of a cu(i)-semiquinone intermediate. to thoroughly examine this possibility, we have measured the spectral changes associated with single-turnover reoxidation by o(2) of sub ... | 2008 | 19053231 |
| hydride transfer made easy in the reaction of alcohol oxidation catalyzed by flavin-dependent oxidases. | choline oxidase (e.c. 1.1.3.17; choline-oxygen 1-oxidoreductase) catalyzes the two-step, four-electron oxidation of choline to glycine betaine with betaine aldehyde as enzyme-associated intermediate and molecular oxygen as final electron acceptor. biochemical, structural, and mechanistic studies on the wild-type and a number of mutant forms of choline oxidase from arthrobacter globiformis have recently been carried out, allowing for the delineation at molecular and atomic levels of the mechanism ... | 2008 | 19053234 |
| substitution of an active site valine uncovers a kinetically slow equilibrium between competent and incompetent forms of choline oxidase. | the enzymatic oxidation of choline to glycine betaine is of interest because organisms accumulate glycine betaine intracellularly in response to stress conditions. this is relevant for the genetic engineering of crops with economic interest that do not naturally possess efficient pathways for the synthesis of glycine betaine and for the potential development of drugs that target the glycine betaine biosynthetic pathway in human pathogens. to date, the best characterized choline-oxidizing enzyme ... | 2008 | 19053262 |
| assessing the importance of dietborne cadmium and particle characteristics on bioavailability and bioaccumulation in the nematode caenorhabditis elegans. | to investigate the impact of multiple cadmium exposure pathways on bioavailability and bioaccumulation, caenorhabditis elegans were exposed to either dissolved cadmium or to both particulate (dietborne) and dissolved cadmium. the dietborne metal exposure scenarios included evaluations of two bacteria species (escherichia coli and arthrobacter globiformis), varying bacteria concentrations, artificial particles (with different surface functional groups or coated with lipopolysaccharides), as well ... | 2009 | 19159127 |
| bacterial communities of tyre monofill sites: growth on tyre shreds and leachate. | to investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria. | 2009 | 19239530 |
| four novel arthrobacter species isolated from filtration substrate. | four gram-positive, non-motile, non-spore-forming bacterial strains, lc4(t), lc6(t), lc10(t) and lc13(t), were isolated from a filtration substrate made from trass, a volcanic rock, and their taxonomic positions were investigated by a polyphasic taxonomic approach. the novel strains grew over a temperature range of 5-40 degrees c, at ph values of 6-11 and in the presence of 3-7 % (w/v) nacl. a phylogenetic tree based on 16s rrna gene sequences showed the novel strains formed a distinct evolution ... | 2009 | 19329620 |
| glycinebetaine-induced water-stress tolerance in coda-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes. | rice (oryza sativa l.), a non-accumulator of glycinebetaine (gb), is highly susceptible to abiotic stress. transgenic rice with chloroplast-targeted choline oxidase encoded by the coda gene from arthrobacter globiformis has been evaluated for inheritance of transgene up to r5 generation and water-stress tolerance. during seedling, vegetative and reproductive stages, transgenic plants could maintain higher activity of photosystem ii and they show better physiological performance, for example, enh ... | 2009 | 19490479 |
| the presence of embedded bacterial pure cultures in agar plates stimulate the culturability of soil bacteria. | traditional methods for bacterial cultivation recover only a small fraction of bacteria from all sorts of natural environments, and attempts have been made to improve the bacterial culturability. here we describe the development of a cultivation method, based on the embedment of pure bacterial cultures in between two layers of agar. plates containing either embedded pseudomonas putida or arthrobacter globiformis resulted in higher numbers of cfus of soil bacteria (21% and 38%, respectively) afte ... | 2009 | 19699242 |
| characteristics and function of alcaligenes sp. nbrc 14130 esterase catalysing the stereo-selective hydrolysis of ethyl chrysanthemate. | alcaligenes sp. nbrc 14130 was found as a strain hydrolysing a mixture of (+/-)-trans- and (+/-)-cis ethyl chrysanthemates to (1r,3r)-(+)-trans-chrysanthemic acid. the alcaligenes cells also have hydrolytic activity for 6-aminohexanoate-cyclic dimer (6-ahcd, 1,8-diazacyclotetradecane-2,9-dione). the correlation of function on the enzyme from the alcaligenes strain with hydrolysis activities for both ethyl chrysanthemate and 6-ahcd was demonstrated. | 2010 | 19778353 |
| [dormant form of micrococcus luteus and arthrobacter globiformis, not growing on standard media]. | | 2009 | 19827710 |
| c, n, and h isotope fractionation of the herbicide isoproturon reflects different microbial transformation pathways. | the fate of pesticides in the subsurface is of great interest to the public, industry, and regulatory authorities. compound-specific isotope analysis (csia) is a promising tool complementary to existing methods for elucidating pesticide degradation reactions. here, we address three different initial biotransformation reactions of the phenylurea herbicide isoproturon (3-(4-isopropylphenyl)-1,1-dimethylurea) in pure culture experiments with bacterial and fungal strains. when analyzing isotopic cha ... | 2010 | 20184312 |
| crystallization and preliminary crystallographic analysis of manganese(ii)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from bacillus sp. jf8. | a thermostable manganese(ii)-dependent 2,3-dihydroxybiphenyl-1,2-dioxygenase derived from bacillus sp. jf8 was crystallized. the initial screening for crystallization was performed by the sitting-drop vapour-diffusion method using a crystallization robot, resulting in the growth of two crystal forms. the first crystal belonged to space group p1, with unit-cell parameters a = 62.7, b = 71.4, c = 93.6 a, alpha = 71.2, beta = 81.0, gamma = 64.0 degrees, and diffracted to 1.3 a resolution. the secon ... | 2010 | 20208161 |
| structural characterization of the major glycolipids from arthrobacter globiformis and arthrobacter scleromae. | arthrobacter is a genus of gram-positive bacteria widely distributed in soil. the ability to catabolize a variety of xenobiotics has shown their potential as a detoxifying agent. recently, arthrobacter has been also recognized as an opportunistic pathogen. glycolipids from a. scleromae, a clinical isolate, and a. globiformis, from soil, were isolated by chloroform-methanol extraction and subsequently purified using column chromatography and high-performance liquid chromatography. structural stud ... | 2010 | 20381794 |
| variability of sediment-contact tests in freshwater sediments with low-level anthropogenic contamination--determination of toxicity thresholds. | freshwater sediments with low levels of anthropogenic contamination and a broad range of geochemical properties were investigated using various sediment-contact tests in order to study the natural variability and to define toxicity thresholds for the various toxicity endpoints. tests were performed with bacteria (arthrobacter globiformis), yeast (saccharomyces cerevisiae), nematodes (caenorhabditis elegans), oligochaetes (lumbriculus variegatus), higher plants (myriophyllum aquaticum), and the e ... | 2010 | 20594629 |
| appraising bacterial strains for rapid bod sensing--an empirical test to identify bacterial strains capable of reliably predicting real effluent bods. | the measured response of rapid biochemical oxygen demand (bod) biosensors is often not identical to those measured using the conventional 5-day bod assay. this paper highlights the efficacy of using both glucose-glutamic acid (gga) and organisation for economic cooperation and development (oecd) bod standards as a rapid screen for microorganisms most likely to reliably predict real effluent bods when used in rapid bod devices. using these two synthetic bod standards, a microorganism was identifi ... | 2010 | 20938773 |
| transgenic brassica chinensis plants expressing a bacterial coda gene exhibit enhanced tolerance to extreme temperature and high salinity. | transgenic brassica compestris l. spp. chinensis plants expressing a choline oxidase (coda) gene from arthrobacter globiformis were obtained through agrobacterium tumefaciens-mediated transformation. in the transgenic plants, coda gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (elisa) examination, immunogold localization, and (1)h-nuclear magnetic resonance ((1)h-nmr). stress tolerance was evaluated in the t(3) plants under e ... | 2010 | 21043054 |
| detection of the reaction intermediates catalyzed by a copper amine oxidase. | to reveal the chemical changes and geometry changes of active-site residues that cooperate with a reaction is important for understanding the functional mechanism of proteins. consecutive temporal analyses of enzyme structures have been performed during reactions to clarify structure-based reaction mechanisms. phenylethylamine oxidase from arthrobacter globiformis (agao) contains a copper ion and topaquinone (tpq(ox)). the catalytic reaction of agao catalyzes oxidative deaminations of phenylethy ... | 2010 | 21169693 |
| cyanobacteria as agents for the control of pollution by pesticides and chlorinated organic compounds. | cyanobacteria are phototrophic aquatic micro-organisms that are found in a variety of environments, including polluted ones. fifteen strains of cyanobacteria that belong to three taxonomic groups are able to degrade lindane (λ-hexachlorocyclohexane, a recalcitrant pesticide). the initial degradation pathway has been studied in two filamentous nitrogen-fixing strains of anabaena sp. pcc 7120 and nostoc ellipsosporum. these cyanobacteria dechlorinated loindane first to pentachlorocyclohexene and t ... | 1998 | 21182708 |
| studies of dimethylglycine oxidase isoenzymes in arthrobacter globiformis cells. | glycine betaine (gb) could be used by arthrobacter globiformis cells as a sole carbon source. the cells took up this molecule in the low as well as in the high salinity medium. addition of gb to the mineral medium with high salt concentration revealed that gb was also used as an osmoprotectant. dimethylglycine oxidase (dmgo) was involved in the catabolism of gb. two genes for dmgo were detected in a cloned 26267 bp fragment of a. globiformis dna. the genes involved in the tetrahydrofolate-depend ... | 2010 | 21188587 |
| structural and enzyme activity studies demonstrate that aryl substituted 2,3-butadienamine analogs inactivate arthrobacter globiformis amine oxidase (agao) by chemical derivatization of the 2,4,5-trihydroxyphenylalanine quinone (tpq) cofactor. | copper amine oxidases (caos) are a family of redox active enzymes containing a 2,4,5-trihydroxyphenylalanine quinone (tpq) cofactor generated from post translational modification of an active site tyrosine residue. the arthrobacter globiformis amine oxidase (agao) has been widely used as a model to guide the design and development of selective inhibitors of caos. in this study, two aryl 2,3-butadienamine analogs, racemic 5-phenoxy-2,3-pentadienylamine (popda) and racemic 6-phenyl-2,3-hexadienyla ... | 2011 | 21215824 |
| identification of the catalytic residues of carboxylesterase from arthrobacter globiformis by diisopropyl fluorophosphate-labeling and site-directed mutagenesis. | the role of amino acid residues in the enzymatic activity of carboxylesterase from arthrobacter globiformis was analyzed by diisopropyl fluorophosphate (dfp) labeling and site-directed mutagenesis. the electrospray ionization mass spectrometric (esi-ms) analysis of the esterase, covalently labeled by dfp, showed stoichiometric incorporation of the inhibitor into the enzyme. the further comparison of endopeptidase-digested fragments between native and dfp-labeled esterase by fast atom bombardment ... | 2011 | 21266781 |
| in vivo self-hydroxylation of an iron-substituted manganese-dependent extradiol cleaving catechol dioxygenase. | the homoprotocatechuate 2,3-dioxygenase from arthrobacter globiformis (mndd) catalyzes the oxidative ring cleavage reaction of its catechol substrate in an extradiol fashion. although this reactivity is more typically associated with non-heme iron enzymes, mndd exhibits an unusual specificity for manganese(ii). mndd is structurally very similar to the iron(ii)-dependent homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum (hpcd), and we have previously shown that both mndd and hpcd are ... | 2011 | 21279661 |
| transformation of tomato with a bacterial coda gene enhances tolerance to salt and water stresses. | genetically engineered tomato (lycopersicon esculentum) with the ability to synthesize glycinebetaine was generated by introducing the coda gene encoding choline oxidase from arthrobacter globiformis. integration of the coda gene in transgenic tomato plants was verified by pcr analysis and dna blot hybridization. transgenic expression of gene was verified by rt-pcr analysis and rna blot hybridization. the coda-transgenic plants showed higher tolerance to salt stress during seed germination, and ... | 2011 | 21342716 |
| ecotoxicity evaluation of selected sulfonamides. | sulfonamides (sas) are a group of antibiotic drugs widely used in veterinary medicine. the contamination of the environment by these pharmaceuticals has raised concern in recent years. however, knowledge of their (eco)toxicity is still very basic and is restricted to just a few of these substances. even though their toxicological analysis has been thoroughly performed and ecotoxicological data are available in the literature, a systematic analysis of their ecotoxicological potential has yet to b ... | 2011 | 21752420 |
| glycinebetaine enhances the tolerance of tomato plants to high temperature during germination of seeds and growth of seedlings. | tomato (lycopersicon esculentum cv. 'moneymaker') was transformed with a coda gene, from arthrobacter globiformis, for choline oxidase that had been modified to allow targeting to both chloroplasts and the cytosol. glycinebetaine (gb) accumulated in seeds of transformed plants up to 1 -ámol g(-1) dw, while no detectable gb was found in wild-type seeds. the coda-transgenic seeds germinated faster and at higher frequency than wild-type seeds with high temperature treatment. after heat stress, leve ... | 2011 | 21711358 |
| molinate hydrolase from gulosibacter molinativorax on4t - a novel cobalt dependent amidohydrolase. | a new pathway of molinate mineralization has been recently described. among the five members of the mixed culture able to promote such a process, gulosibacter molinativorax on4(t) has been observed to promote the initial breakdown of the herbicide into ethanethiol and azepane-1-carboxylate. in the current study, the gene encoding the enzyme responsible for molinate hydrolysis was identified, heterologously expressed and the resultant active protein purified and characterized. nucleotide sequence ... | 2011 | 21840982 |
| Structural insights into the substrate specificity of bacterial copper amine oxidase obtained by using irreversible inhibitors. | Copper amine oxidases (CAOs) catalyze the oxidation of various aliphatic amines to the corresponding aldehydes, ammonia and hydrogen peroxide. Although CAOs from various organisms share a highly conserved active-site structure including a protein-derived cofactor, topa quinone (TPQ), their substrate specificities differ considerably. To obtain structural insights into the substrate specificity of a CAO from Arthrobacter globiformis (AGAO), we have determined the X-ray crystal structures of AGAO ... | 2011 | 21984603 |
| cloning of inulin fructotransferase (dfa iii-producing) gene from arthrobacter sp. l68-1. | a gene of inulin fructotransferase (dfa iii-producing) [ec 2.4.1.93] from arthrobacter sp. l68-1 was cloned and the nucleotide was sequenced. the gene encoded a signal peptide (32 amino acid residues) for a secretion, and the mature enzyme protein was estimated to be consisted with 410 amino acid residues. the molecular mass of the native enzyme was calculated as 43.7kda by the sequence data. the deduced amino acid sequence of the enzyme had 79.0% homology with that of the arthrobacter globiform ... | 2012 | 23499085 |
| microbiology of the gut of the kola nut weevil, balanogastris kolae. | reports have shown that many insects have microbes in their gut system. gut microbes are very important for insect vitality and much of their nutrition is derived from products of microbial metabolism. the habitat of balanogastris kolae (desbrocher des loges) (coleoptera: curculionidae) suggests that they possess the ability to digest varieties of sugars particularly starch and protein materials present in the kola nut, cola nitida schott & endlicher (malvales: malvaceae). the aim of this study ... | 2012 | 23421598 |
| probing the catalytic mechanism of copper amine oxidase from arthrobacter globiformis with halide ions. | the catalytic reaction of copper amine oxidase proceeds through a ping-pong mechanism comprising two half-reactions. in the initial half-reaction, the substrate amine reduces the tyr-derived cofactor, topa quinone (tpq), to an aminoresorcinol form (tpqamr) that is in equilibrium with a semiquinone radical (tpqsq) via an intramolecular electron transfer to the active-site copper. we have analyzed this reductive half-reaction in crystals of the copper amine oxidase from arthrobacter globiformis. a ... | 2015 | 26269595 |
| distribution of epiphytic bacteria on olive leaves and the influence of leaf age and sampling time. | mesophilic heterotrophic, aerobic or facultatively anaerobic bacteria that grow on yeast tryptone glucose extract agar were isolated from the surface of olive leaves of 3 or 4 different ages in january, april, july, and october from 1984 to 1989. unweighted average linkage cluster analysis on either the jaccard coefficient or the simple matching coefficient recovered 1,701 representative strains in 32 phena defined at the 70% and 80% similarity level, respectively. of these, 25 were identified t ... | 1991 | 24194200 |
| arthrobacter pokkalii sp nov, a novel plant associated actinobacterium with plant beneficial properties, isolated from saline tolerant pokkali rice, kerala, india. | a novel yellow colony-forming bacterium, strain p3b162t was isolated from the pokkali rice rhizosphere from kerala, india, as part of a project study aimed at isolating plant growth beneficial rhizobacteria from saline tolerant pokkali rice and functionally evaluate their abilities to promote plant growth under saline conditions. the novel strain p3b162t possesses plant growth beneficial traits such as positive growth on 1-aminocyclopropane-1-carboxylic acid (acc), production of indole acetic ac ... | 2016 | 26963092 |
| arthrobacter nanjingensis sp. nov., a mineral-weathering bacterium isolated from forest soil. | a gram-stain-positive, non-motile, rod- or coccoid-shaped actinobacterium, designated strain a33(t), was isolated from a forest soil sample from nanjing, jiangsu province, pr china. the strain grew optimally at 30 °c, ph 7.0 and with 3 % nacl (w/v). phylogenetic analysis of the strain, based on 16s rrna gene sequences, showed that it was most closely related to arthrobacter woluwensis (98.4 % sequence similarity), arthrobacter humicola (97.5 %), arthrobacter globiformis (97.4 %), arthrobacter or ... | 2015 | 25358511 |
| crystal structure of bacillus fastidious uricase reveals an unexpected folding of the c-terminus residues crucial for thermostability under physiological conditions. | bacillus fastidious uricase (bf uricase) containing 322 amino acid residues exhibited high stability under physiological conditions. its crystal structure was solved to 1.4-å resolution, showing homotetramer containing two homodimers. after the intersubunit antiparallel β-sheet in its homodimer, each subunit had a total of 18 c-terminus residues forming an α-helix (q305-a313) and random coil (s314-l322) on surface to bury other two α-helices (i227-t238 and i244-r258). in comparison, reported cry ... | 2015 | 25786739 |
| evidence for cooperative mineralization of diuron by arthrobacter sp. bs2 and achromobacter sp. sp1 isolated from a mixed culture enriched from diuron exposed environments. | diuron was found to be mineralized in buffer strip soil (bs) and in the sediments (sed) of the morcille river in the beaujolais vineyard repeatedly treated with this herbicide. enrichment cultures from bs and sed samples led to the isolation of three bacterial strains transforming diuron to 3,4-dichloroaniline (3,4-dca) its aniline derivative. 16s rrna sequencing revealed that they belonged to the genus arthrobacter (99% of similarity to arthrobacter globiformis strain k01-01) and were designate ... | 2014 | 25061887 |
| [cloning and characterization of a thermostable urate oxidase from microbacterium sp. strain zzj4-1]. | in order to characterize a thermostable urate oxidase (uox) from microbacterium sp. strain zzj4-1, we cloned its gene (uox). the open reading frame of uox contained 894 base pairs and encoded a protein with 297 amino acids. alignment of gene sequences indicated there was no obvious identity with the most reported uox and that 72% identity was found with uox from arthrobacter globiformis. we inserted the gene into the plasmid pet-15b to construct an expression vector pet-15b-uox and got it induce ... | 2012 | 23167193 |
| purification and characterization of an alkaliphilic choline oxidase of fusarium oxysporum. | a novel choline oxidase found in a fungus, fusarium oxysporum strain v2, was purified to homogeneity as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. the enzyme has a molecular mass of 128 kda and consists of two identical subunits. the purified enzyme showed adsorption peaks at 340 nm and 450 nm. it showed alkaliphilic ph characteristics: its optimum ph was 9.0-10.0, and it was stable at ph 8.0-10.2. the michaelis constant (km) values for choline and betaine aldehyde ... | 2012 | 23221722 |
| taxonomic studies on arthrobacter nicotianae and related taxa: description of arthrobacter uratoxydans sp. nov. and arthrobacter sulfureus sp. nov. and reclassification of brevibacterium protophormiae as arthrobacter protophormiae comb. nov. | dna homology studies were performed on various strains of arthrobacter and related organisms with peptidoglycans (mureins) of the a3α and a4α variations. homology values above 20% show strains with the a4α variation to form a coherent group, which is here designated as the "nicotianae" group of organisms. members of this group show homology values of lower than 20% with strains of the "globiformis" group (arthrobacter globiformis and relatives), having a a3α peptidoglycan variation. however, the ... | 1983 | 23194805 |
| assessing the ecological long-term impact of wastewater irrigation on soil and water based on bioassays and chemical analyses. | the reuse of treated wastewater for irrigation and groundwater recharge can counteract water scarcity and reduce pollution of surface waters, but assessing its environmental risk should likewise consider effects associated to the soil. the present study therefore aimed at determining the impact of wastewater irrigation on the habitat quality of water after soil passage and of soil after percolation by applying bioassays and chemical analysis. lab-scale columns of four different soils encompassin ... | 2015 | 26207878 |
| evaluation of the hazard potentials of river suspended particulate matter and floodplain soils in the rhine basin using chemical analysis and in vitro bioassays. | the purpose of the present study was to assess the hazard potentials of contaminated suspended particulate matter (spm) sampled during a flood event for floodplain soils using in vitro bioassays and chemical analysis. sediment-contact tests were performed to evaluate the direct exposure of organisms to native soils and spm at two different trophic levels. for comparison, acetonic extracts were tested using both contact tests and additionally two cell-based biotests for cytotoxicity and ah recept ... | 2015 | 25331527 |
| sediment contact tests as a tool for the assessment of sediment quality in german waters. | a sediment contact test (sct) battery consisting of five ecotoxicological test systems was applied to 21 native freshwater sediments characterized by a broad variety of geochemical properties and anthropogenic contamination. higher plants (myriophyllum aquaticum), nematodes (caenorhabditis elegans), oligochaetes (lumbriculus variegatus), zebrafish embryos (danio rerio), and bacteria (arthrobacter globiformis), representing various trophic levels and exposure pathways, were used as test organisms ... | 2013 | 23027525 |