| bacterial strain-specific induction of foxp3+ t regulatory cells is protective in murine allergy models. | the incidence of atopic disease has increased dramatically during recent decades and the potential immunoregulatory influence of the microbiota in these individuals is under investigation. | 2010 | 20067483 |
| Production of Multiple Bacteriocins from a Single Locus by Gastrointestinal Strains of Lactobacillus salivarius. | Bacteriocins produced by Lactobacillus salivarius isolates derived from a gastrointestinal origin have previously demonstrated efficacy for in vivo protection against Listeria monocytogenes infection. In this study, comparative genomic analysis was employed to investigate the intraspecies diversity of seven L. salivarius isolates of human and porcine intestinal origin, based on the genome of the well-characterized bacteriocin-producing strain L. salivarius UCC118. This revealed a highly conserve ... | 2011 | 21984788 |
| lactic acid bacteria from chicken carcasses with inhibitory activity against salmonella spp. and listeria monocytogenes. | this study was conducted to isolate psychrotrophic lactic acid bacteria (lab) from chicken carcasses with inhibitory activity against strains of salmonella spp. and listeria monocytogenes. a total of 100 broiler samples were examined for the presence of lab. ninety-two lab isolates that showed antimicrobial effects against salmonella spp. and l. monocytogenes were further analysed to examine their lab (gram-positive, catalase negative, oxidase negative) and psychrotrophic characteristics (abilit ... | 2011 | 21983108 |
| genome sequence of lactobacillus salivarius nias840, isolated from chicken intestine. | lactobacillus salivarius is a well-known lactic acid bacterium to which increasing attention has been paid recently for use as probiotics for humans and animals. l. salivarius nias840 was first isolated from broiler chicken feces, displaying antimicrobial activities against multidrug-resistant staphylococcus aureus and salmonella enterica serovar typhimurium. here, we report the genome sequence of l. salivarius nias840 (2,046,557 bp) including a small plasmid and two megaplasmids. | 2011 | 21914873 |
| estimation of combined effects of carbon and nitrogen sources on the growth and bacteriocin production of lactobacillus salivarius from human source. | vaginal lactobacillus salivarius crl 1328 (centro de referencia para lactobacilos culture collection) or the bacteriocin that produces could be included in a urogenital probiotic formula to prevent urogenital infections in women. the objective of this work was to determine the effects of different carbon and nitrogen sources on the growth and bacteriocin production of this microorganism. a fractional factorial design 3(5-1) was applied to evaluate the effects of five nutrients (glucose, lactose, ... | 2010 | 20082367 |
| strain-dependent release of cytokines modulated by lactobacillus salivarius human isolates in an in vitro model. | abstract: | 2010 | 20184725 |
| in vitro immunomodulatory activity of lactobacillus fermentum cect5716 and lactobacillus salivarius cect5713: two probiotic strains isolated from human breast milk. | commensal bacteria, including some species of lactobacilli commonly present in human breast milk, appear to colonize the neonatal gut and contribute to protection against infant infections, suggesting that lactobacilli could potentially modulate immunity. in this study, we evaluated the potential of two lactobacillus strains isolated from human milk to modulate the activation and cytokine profile of peripheral blood mononuclear cell (pbmc) subsets in vitro. moreover, these effects were compared ... | 2010 | 20219262 |
| screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties. | lactic acid bacteria originated from swine feces and intestines were selected for potential probiotics based on their bile-salt resistance, low ph tolerance, potential adhesion to epithelial cells and especially functional properties, including production of antimicrobial substances, bile-salt hydrolase (bsh) and amylolytic activity. results showed 7 isolates with antimicrobial activity, 5 with bsh activity and 3 with amylolytic activity were preliminarily selected from 485 lactic acid bacteria ... | 2010 | 20304081 |
| identification of lactobacilli residing in chicken ceca with antagonism against campylobacter. | bacteriocins produced by lactobacillus salivarius have been recently recognized as a natural means to control campylobacter and salmonella in live poultry. this finding is of relevance since campylobacter jejuni and campylobacter coli are the predominant species isolated from poultry that are associated with human campylobacteriosis. in the present work, lactic acid bacteria (lab) isolated from the cecum of twenty tunisian chickens were identified and those isolates with antagonism against campy ... | 2011 | 22069154 |
| probiotic colonization of the adherent mucus layer of ht29mtxe12 cells attenuates campylobacter jejuni virulence properties. | the ht29mtxe12 (e12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. in this study, we have characterized the interaction of campylobacter jejuni with the e12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate c. jejuni virulence properties. c. jejuni 81-176 colonized and reproduced in e12 mucus. adhesion to and internalization of c. jejuni were enhanced in e12 cells harb ... | 2010 | 20308300 |
| glutathione-mediated response to acid stress in the probiotic bacterium, lactobacillus salivarius. | lactobacillus salivarius, a probiotic bacterium, encounters acidic conditions in its passage through the gastrointestinal tract of human and animal hosts. we studied the effect of a rapid downshift in extracellular ph from 6.5 to 4 on cell growth. the maximum growth rate was higher in low ph medium with glutathione supplementation than without. cells developed a gsh-mediated acid-tolerance response and, when grown with 0.5 mm gsh, reached a higher final density than with other conditions. these ... | 2010 | 20349113 |
| protein-carbohydrate interactions between lactobacillus salivarius and pig mucins. | adherence to the gastrointestinal tract is a key element desirable for many of the proposed beneficial health effects of probiotic bacteria. the aims of this study were to determine the levels of adhesion of 3 lactobacillus salivarius strains (lb6, lb9, and lb10) to porcine small intestine mucins and to determine whether adhesion is a function of lectin-like activities. dot and western blot assays were performed to investigate bacterial adhesion. several carbohydrates and glycoproteins were eval ... | 2011 | 21622872 |
| lactobacilli antagonize the growth, motility, and adherence of brachyspira pilosicoli: a potential intervention against avian intestinal spirochetosis. | avian intestinal spirochetosis (ais) results from the colonization of the ceca and colorectum of poultry by pathogenic brachyspira species. the number of cases of ais has increased since the 2006 european union ban on the use of antibiotic growth promoters, which, together with emerging antimicrobial resistance in brachyspira, has driven renewed interest in alternative intervention strategies. probiotics have been reported as protecting livestock against infection with common enteric pathogens, ... | 2011 | 21666022 |
| comparative evaluation of automated ribotyping and rapd-pcr for typing of lactobacillus spp. occurring in dental caries. | a group of 67 lactobacillus spp. strains containing lactobacillus casei/paracasei, lactobacillus fermentum, lactobacillus gasseri, lactobacillus plantarum, lactobacillus rhamnosus and lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previous study (svec et al., folia microbiol 54:53-58, 2009) was characterized by automated ribotyping performed by the riboprinter microbial characterization system and by randomly amplified polymorphic d ... | 2010 | 20361257 |
| methodological issues in randomized trials assessing probiotics for periodontal treatment. | dhingra k. methodological issues in randomized trials assessing probiotics for periodontal treatment. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01399.x.©2011 john wiley & sons a/s background and objective: probiotics traditionally used in medicine field are now being used in an attempt to control and treat periodontal disease. however, the trials used to analyze the effects of probiotics have been subject to methodological criticism. the aim of this review was to assess the methodolog ... | 2011 | 21777405 |
| inducer bacteria, unique signal peptides, and low-nutrient media stimulate in vitro bacteriocin production by lactobacillus spp. and enterococcus spp. strains. | bacteriocins (bcn) are antimicrobial peptides that provide potential to control bacterial infections in a variety of applications. we previously reported on three class iia bcn molecules produced by lactobacillus salivarius b-30514 (or-7), enterococcus faecium b-30746 (e 50-52), and enterococcus durans/faecium/hirae b-30745 (e-760). these bcn are notably effective against a wide array of pathogenic bacteria. to commercially apply such bcn, adequate quantities must be produced and harvested. we d ... | 2010 | 20429596 |
| luminal administration ex vivo of a live lactobacillus species moderates mouse jejunal motility within minutes. | gut commensals modulate host immune, endocrine, and metabolic functions. they also affect peripheral and central neural reflexes and function. we have previously shown that daily ingestion of lactobacillus reuteri (lr) for 9 d inhibits the pseudoaffective cardiac response and spinal single-fiber discharge evoked by visceral distension, and decreases intestinal motility and myenteric ah cell slow afterhyperpolarization (sahp) by inhibiting a ca-activated k (ik(ca)) channel. we tested whether lumi ... | 2010 | 20519636 |
| surface glycosaminoglycans mediate adherence between hela cells and lactobacillus salivarius lv72. | the adhesion of lactobacilli to the vaginal surface is of paramount importance to develop their probiotic functions. for this reason, the role of hela cell surface proteoglycans in the attachment of lactobacillus salivarius lv72, a mutualistic strain of vaginal origin, was investigated. | 2013 | 24044741 |
| genome sequence of lactobacillus salivarius gj-24, a probiotic strain isolated from healthy adult intestine. | the draft genome sequence of lactobacillus salivarius gj-24 isolated from the feces of healthy adults was determined. its properties, including milk fermentation activity and bacteriocin production, suggest its potential uses as a probiotic lactic acid bacterium and start culture for dairy products. | 2011 | 21742893 |
| bactofencin a, a new type of cationic bacteriocin with unusual immunity. | bacteriocin production is an important probiotic trait of intestinal bacteria. in this study, we identify a new type of bacteriocin, bactofencin a, produced by a porcine intestinal isolate lactobacillus salivarius dpc6502, and assess its potency against pathogenic species including staphylococcus aureus and listeria monocytogenes. genome sequencing of the bacteriocin producer revealed bfna, which encodes the mature and highly basic (pi 10.59), 22-amino-acid defensin-like peptide. matrix-assisted ... | 2013 | 24169573 |
| analysis of the human intestinal epithelial cell transcriptional response to lactobacillus acidophilus, lactobacillus salivarius, bifidobacterium lactis and escherichia coli. | the complex microbial population residing in the human gastrointestinal tract consists of commensal, potential pathogenic and beneficial species, which are probably perceived differently by the host and consequently could be expected to trigger specific transcriptional responses. here, we provide a comparative analysis of the global in vitro transcriptional response of human intestinal epithelial cells to lactobacillus acidophilus ncfmôäó, lactobacillus salivarius ls-33, bifidobacterium animalis ... | 2010 | 21831765 |
| heterologous surface display on lactic acid bacteria: non-gmo alternative? | lactic acid bacteria (lab) are food-grade hosts for surface display with potential applications in food and therapy. alternative approaches to surface display on lab would avoid the use of recombinant dna technology and genetically-modified organism (gmo)-related regulatory requirements. non-covalent surface display of proteins can be achieved by fusing them to various cell-wall binding domains, of which the lysine motif domain (lysm) is particularly well studied. fusion proteins have been isola ... | 2015 | 25880164 |
| structure of ribose 5-phosphate isomerase from the probiotic bacterium lactobacillus salivarius ucc118. | the structure of ribose 5-phosphate isomerase from the probiotic bacterium lactobacillus salivarius ucc188 has been determined at 1.72 å resolution. the structure was solved by molecular replacement, which identified the functional homodimer in the asymmetric unit. despite only showing 57% sequence identity to its closest homologue, the structure adopted the typical α and β d-ribose 5-phosphate isomerase fold. comparison to other related structures revealed high homology in the active site, allo ... | 2012 | 23192019 |
| oral treatment of chickens with lactobacilli influences elicitation of immune responses. | commensal microbes in the intestine are in constant interaction with host cells and play a role in shaping the immune system. lactobacillus acidophilus, lactobacillus reuteri, and lactobacillus salivarius are members of the chicken intestinal microbiota and have been shown to induce different cytokine profiles in mononuclear cells in vitro. the objective of the present study was to examine the effects of these bacteria individually or in combination on the induction of antibody- and cell-mediate ... | 2011 | 21734067 |
| characterization of lactobacillus salivarius alanine racemase: short-chain carboxylate-activation and the role of a131. | many strains of lactic acid bacteria produce high concentrations of d-amino acids. among them, lactobacillus salivarius ucc 118 produces d-alanine at a relative concentration much greater than 50 % of the total d, l-alanine (100d/d, l-alanine). we characterized the l. salivarius alanine racemase (alr) likely responsible for this d-alanine production and found that the enzyme was activated by carboxylates, which is an unique characteristic among alrs. in addition, alignment of the amino acid sequ ... | 2015 | 26543773 |
| anti-inflammatory properties of probiotic bacteria on salmonella-induced il-8 synthesis in enterocyte-like caco-2 cells. | invasion of the gut by pathogenic salmonella leads to production of il-8 that initiates inflammatory reactions to combat the bacterium. however, its persistent production causes tissue damage and interventions that suppress il-8 production prevent tissue damage. we hypothesised that probiotics could mediate their benefits via inhibition of il-8 synthesis. caco-2 cells were infected with probiotic bifidobacterium infantis w52, lactobacillus casei w56, lactococcus lactis w58, lactobacillus acidoph ... | 2010 | 21840800 |
| Characterization of tetracycline resistance lactobacilli isolated from swine intestines at western area of Taiwan. | To investigate the frequency of tetracycline resistance (Tet-R) lactobacilli in pig intestines, a total of 256 pig colons were analyzed and found to contain typical colonies of Tet-R lactic acid bacteria in every sample, ranging from 3.2 × 10(3) to 6.6 × 10(5) CFU/cm(2). From these samples, a total of 159 isolates of Tet-R lactobacilli were obtained and identified as belonging to 11 species, including Lactobacillus reuteri, Lactobacillus amylovorus, Lactobacillus salivarius, Lactobacillus planta ... | 2011 | 21906691 |
| in vitro assessment of the immunomodulatory effects of multispecies probiotic formulations for management of allergic diseases. | modulation of the composition of the intestinal microbiota with probiotics could possibly offer a way of prevention or management of allergic diseases. the objective of this study was to determine the immunomodulating effects of various multispecies probiotic combinations in vitro, as preamble to application in vivo. multispecies probiotic combinations were formulated and tested for their effects on in vitro cytokine production by human mononuclear cells and were compared to products that alread ... | 2011 | 21986357 |
| development and analysis of microbial characteristics of an acidulocomposting system for the treatment of garbage and cattle manure. | an acidulocomposting system for the treatment of cattle manure with little emission of ammonia gas was developed, and the structure of its microbial community was investigated by denaturing gradient gel electrophoresis (dgge) and clone library construction. an acidulocomposting apparatus (bc20, 20 l) was operated for 79 days to treat 2 kg (wet wt) of garbage per 1 or 2 days. on day 80 of operation, the substrate was changed from garbage to cattle manure (1 kg of beef cattle manure was added to t ... | 2010 | 20547374 |
| carbohydrate catabolic flexibility in the mammalian intestinal commensal lactobacillus ruminis revealed by fermentation studies aligned to genome annotations. | lactobacillus ruminis is a poorly characterized member of the lactobacillus salivarius clade that is part of the intestinal microbiota of pigs, humans and other mammals. its variable abundance in human and animals may be linked to historical changes over time and geographical differences in dietary intake of complex carbohydrates. | 2011 | 21995520 |
| mucoadhesive alginate/poly (l-lysine)/thiolated alginate microcapsules for oral delivery of lactobacillus salivarius 29. | in this study, thiolated alginate was synthesized by introduction of cysteine to alginate to prepare mucoadhesive alginate/poly (l-lysine)/thiolated alginate (apta) microcapsules for efficient oral delivery of lactobacillus salivarius 29 (ls29), a novel therapeutic lactobacillus strain, in vitro and in vivo. about 759 +/- 32.4 microm of cysteine per gram of alginate was introduced by estimation of ellman's reagent reaction. ls29-loaded apta microcapsules provided suitable morphology, size, and a ... | 2011 | 22103131 |
| pcr-based identification of eight lactobacillus species and 18 hr-hpv genotypes in fixed cervical samples of south african women at risk of hiv and bv. | vaginal lactobacilli assessed by pcr-based microarray and pcr-based genotyping of hpv in south african women at risk for hiv and bv. vaginal lactobacilli can be defined by microarray techniques in fixed cervical samples of south african women. cervical brush samples suspended in the coagulant fixative boonfix of one hundred women attending a health centre for hiv testing in south africa were available for this study. in the ndlovu medical centre in elandsdoorn, south africa, identification of 18 ... | 2011 | 22021225 |
| oral administration of lactobacillus salivarius inhibits the allergic airway response in mice. | asthma is recognized throughout the world as a chronic airway inflammatory disease. in this study, we investigated the effect of probiotics in response to antigen challenge in an ovalbumin (ova)-sensitized asthma model in balb/c mice. lactobacillus salivarius pm-a0006 was orally administered to mice before antigen challenge. after antigen challenge, serum ova-specific antibody levels, airway responsiveness to methacholine, influx of inflammatory cells to the lung, and cytokine levels in bronchoa ... | 2010 | 20555399 |
| putative probiotic lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens. | a total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible gastroenteritis (tge) coronavirus and other enteric pathogens. based on activity, the strains probio-38 and probio-37 were selected as potential probiotics and identified as lactobacillus plantarum probio-38 and lactobacillus salivarius probio-37 respectively by 16s rrna gene sequencing. supernatants of these strains inhibited tge coronavirus in vitro in st cells ... | 2010 | 20623187 |
| diversity of lactobacillus and bifidobacterium in feces of herbivores, omnivores and carnivores. | the lactobacillus and bifidobacterium population in the feces of 26 animals (16 species) were studied by culture-dependent and culture-independent techniques. lactobacilli were detected from a few herbivores, all carnivores and some omnivores. lactobacillus johnsonii, lactobacillus reuteri, lactobacillus salivarius, lactobacillus vaginalis and lactobacillus ingluviei were the most dominant lactobacilli in carnivores. these species were, however, not predominant in herbivores and omnivores. lacto ... | 2010 | 21034840 |
| modulatory effects of lactobacillus salivarius on intestinal mucosal immunity of piglets. | recent studies have demonstrated that lactobacilli or their cell components can improve certain immune function in animals. the aim of this study is to investigate the effects of porcine lactobacilli on the intestinal mucosal immunity of piglets. neonatal piglets were used as a model and were orally administrated with lactobacillus salivarius b1 isolated from the duodenal mucosa of a healthy piglet. the feces of the piglets were collected on days 7, 14, and 21 for intestinal microflora analysis. ... | 2011 | 21365445 |
| anti-inflammatory capacity of selected lactobacilli in experimental colitis is driven by nod2-mediated recognition of a specific peptidoglycan-derived muropeptide. | background and aims inflammatory bowel disease (ibd) has been linked to a loss of tolerance towards the resident microflora. therapeutic use of probiotics is known to be strain specific, but precise mechanisms remain unclear. the role of nod2 signalling and the protective effect of lactobacillus peptidoglycan (pgn) and derived muropeptides in experimental colitis were evaluated. methods the anti-inflammatory capacity of lactobacilli and derived bacterial compounds was evaluated using the 2,4,6-t ... | 2011 | 21471573 |
| effect of glutathione on growth of the probiotic bacterium lactobacillus reuteri. | glutathione (gsh) is an abundant nonprotein thiol that plays numerous roles within the cell. previously, we showed that lactobacillus salivarius has the capacity to mount a glutathione-mediated acid-tolerance response. in the present work we provide evidence of a requirement for gsh by lactobacillus reuteri and have studied the role of gsh during cell growth. medium supplementation with 0.5 mm gsh as the sole sulfur source enhanced cell growth, resulting in an increase in glucose consumption, an ... | 2011 | 21585317 |
| Oral and faecal lactobacilli and their expression of mannose-specific adhesins in individuals with and without IgA deficiency. | Lactobacilli are present in the intestine and oral cavity of most adults. Secretory IgA in mucosal secretions may provide carbohydrate receptors for bacterial adhesins. Here, oral and faecal samples from 33 IgA-deficient individuals and 34 controls were cultured for lactobacilli, which were identified using species-specific PCR or partial 16S rDNA sequencing and tested for expression of mannose-specific adhesins. Lactobacilli were found in the oral cavity of 76% of IgA-deficient and 85% of contr ... | 2012 | 22018813 |
| heat-killed lactic acid bacteria enhance immunomodulatory potential by skewing the immune response toward th1 polarization. | heat-killed lactic acid bacteria not only possess immunomodulatory functions but also provide the advantages of longer product shelf life, easier storage, and more convenient transportation. to establish appropriate heat treatments for the industrial preparation of probiotics with immunomodulatory effects, 4 different heat treatments were used to kill 11 strains of lactic acid bacteria. comparisons among the strains and with viable forms were carried out in terms of immunomodulatory activity and ... | 2011 | 22417436 |
| isolation and characterization of lactobacillus salivarius mtc 1026 as a potential probiotic. | researchers are becoming more interested in studying probiotics at present due to their benefit to human and animal health. in this study, newly isolated strains from human feces were evaluated for probiotic properties. a total of sixty isolated strains were collected from feces and six out of sixty isolated strains could inhibit the growth of salmonella typhi and salmonella typhimurium. the strain which gave the best inhibitory effect was selected for further characterization as a probiotic str ... | 2011 | 22353742 |
| acid production and growth by oral lactobacillus species in vitro. | to analyze the acid-producing and growth abilities of different oral lactobacillus species. | 2011 | 22298522 |
| effects of lactobacillus salivarius ls01 (dsm 22775) treatment on adult atopic dermatitis: a randomized placebo-controlled study. | atopic dermatitis (ad) is a common inflammatory skin disease characterized by xerosis, pruritus and eczema. the role of probiotics in the prevention and the treatment of ad have been extensively studied in children with controversial results while there are few studies on an adult population. the aim of this randomized, double-blind, placebo-controlled study is to evaluate the clinical efficacy of the intake of a probiotic strain (lactobacillus salivarius ls01) in the treatment of adult patients ... | 2011 | 22230409 |
| bugs that debugs: probiotics. | the oral cavity harbors a diverse array of bacterial species. there are more than 600 species that colonize in the oral cavity. these include a lot of organisms that are not commonly known to reside in the gastrointestinal (gi) tract and also are more familiar: lactobacillus acidophilus, lactobacillus casei, lactobacillus fermentum, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus salivarius. the balance of all these microorganisms can easily be disturbed and a prevalence of p ... | 2012 | 23066281 |
| high-resolution structures of lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate. | probiotic bacterial strains have been shown to enhance the health of the host through a range of mechanisms including colonization, resistance against pathogens, secretion of antimicrobial compounds and modulation of the activity of the innate immune system. lactobacillus salivarius ucc118 is a well characterized probiotic strain which survives intestinal transit and has many desirable host-interaction properties. probiotic bacteria display a wide range of catabolic activities, which determine t ... | 2015 | 26457526 |
| characterization of antimicrobial substance from lactobacillus salivarius kl-d4 and its application as biopreservative for creamy filling. | lactobacillus salivarius kl-d4 isolated from duck intestine produced bacteriocin which was stable at high temperature and a wide ph range of 3-10. its cell free supernatant at ph 5.5 exhibited wide inhibitory spectrum against both g+ and g- bacteria. the highest bacteriocin production was obtained in mrs broth supplemented with 0.5 % (w/v) caco3 at 6 h by gentle shaking. pcr walking using specific primers at the conserved region of class-ii bacteriocin resulted in 4 known genes of kld1, kld2, kl ... | 2016 | 27462508 |
| microbial inhibition of oral epithelial wound recovery: potential role for quorum sensing molecules? | awareness of the impact of microbiota in both health and disease is growing. using a new in vitro oral mucosa co-culture model, we recently showed a clear inhibition of epithelial wound healing in the presence of an oral microbial community. in this paper, we have used the same model in combination with specific oral microbial species to obtain a better insight into the role of the oral microbiota in wound healing. monocultures of klebsiella oxytoca and lactobacillus salivarius significantly inh ... | 2015 | 25995981 |
| identification of lactobacillus strains with probiotic features from the bottlenose dolphin (tursiops truncatus). | in order to develop complementary health management strategies for marine mammals, we used culture-based and culture-independent approaches to identify gastrointestinal lactobacilli of the common bottlenose dolphin, tursiops truncatus. | 2013 | 23855505 |
| unusual genome complexity in lactobacillus salivarius jcm1046. | lactobacillus salivarius strains are increasingly being exploited for their probiotic properties in humans and animals. dissemination of antibiotic resistance genes among species with food or probiotic-association is undesirable and is often mediated by plasmids or integrative and conjugative elements. l. salivarius strains typically have multireplicon genomes including circular megaplasmids that encode strain-specific traits for intestinal survival and probiotic activity. linear plasmids are le ... | 2014 | 25201645 |
| identification of a novel epoxyqueuosine reductase family by comparative genomics. | the reduction of epoxyqueuosine (oq) is the last step in the synthesis of the trna modification queuosine (q). while the epoxyqueuosine reductase (ec 1.17.99.6) enzymatic activity was first described 30 years ago, the encoding gene queg was only identified in escherichia coli in 2011. interestingly, queg is absent from a large number of sequenced genomes that harbor q synthesis or salvage genes, suggesting the existence of an alternative epoxyqueuosine reductase in these organisms. by analyzing ... | 2017 | 28128549 |
| the comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study. | this study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (eep) to chlorhexidine gluconate (chx) on planktonic streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus, lactobacillus salivarius subsp. salivarius, aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, staphylococcus aureus, enterococcus faecalis, actinomyces israelii, candida albicans, and their single-species biofilms by agar dilution and broth mi ... | 2016 | 26949701 |
| antibacterial effects of n-acetylcysteine against endodontic pathogens. | the success of endodontic treatment depends on the eradication of microorganisms from the root canal system and the prevention of reinfection. the purpose of this investigation was to evaluate the antibacterial and antibiofilm efficacy of n-acetylcysteine (nac), an antioxidant mucolytic agent, as an intracanal medicament against selected endodontic pathogens. minimum inhibitory concentrations (mics) of nac for actinomyces naeslundii, lactobacillus salivarius, streptococcus mutans, and enterococc ... | 2016 | 27033208 |
| strains of enterococcus faecalis differ in their ability to coexist in biofilms with other root canal bacteria. | to investigate the relationship between protease production and the ability of enterococcus faecalis strains to coexist in biofilms with other bacteria commonly recovered from infected root canals. | 2015 | 26172346 |
| antibacterial efficacy of a human β-defensin-3 peptide on multispecies biofilms. | the aggregation of mixed bacterial flora into sessile biofilms on root canal surfaces can be one of the causes of persistent apical periodontitis. the aim of this study was to evaluate the antibacterial efficacy of human β-defensin-3 (hbd3) peptide on multispecies biofilms by using confocal laser scanning microscopy. | 2013 | 24238461 |
| Lactobacillus salivarius plus fructo-oligosaccharide is superior to fructo-oligosaccharide alone for treating children with moderate to severe atopic dermatitis: a double-blind, randomized, clinical trial of efficacy and safety. | Background Some probiotics can ameliorate childhood atopic dermatitis (AD). Prebiotics have also shown some efficacy, although when combined with probiotics as synbiotics, their efficacy may improve. Objective We compared the effects of Lactobacillus salivarius and fructo-oligosaccharide (synbiotic) with fructo-oligosaccharide alone (prebiotic) on children with moderate to severe AD. Methods We randomly assigned 60 children aged 2-14 years with moderate to severe AD [SCORing AD (SCORAD) > 25] ... | 2011 | 21895621 |
| probiotic gut effect prevents the chronic psychological stress-induced brain activity abnormality in mice. | a probiotic formulation (lactobacillus helveticus r0052 and bifidobacterium longum r0175 combination, probio'stick(®) ) displays anxiolytic-like activity and reduces apoptosis in the lymbic system in animal models of depression. based on the hypothesis that modulation of gut microbiota by this probiotic formulation has beneficial effects on brain activity in stress conditions, we report a set of probiotic-evoked physiological, cellular, and molecular events in the brain of probio'stick(®) pretre ... | 2014 | 24372793 |
| effect of metalloporphyrins on red autofluorescence from oral bacteria. | the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ... | 2013 | 23659237 |
| Lactobacillus salivarius REN counteracted unfavorable 4-nitroquinoline-1-oxide-induced changes in colonic microflora of rats. | Probiotics and carcinogens both have a significant effect on the microfloral composition of the human intestine. The objective of this study was to investigate the impact of an important carcinogen, 4-Nitroquinoline-1-Oxide on colonic microflora and the efficacy of the probiotic Lactobacillus salivarius REN as an agent of counteracting these effects. Using denaturing gradient gel electrophoresis (DGGE) combined with redundancy analysis, we demonstrated that both 4-Nitroquinoline-1-Oxide and L. s ... | 2011 | 22203549 |
| development of a multispecies biofilm community by four root canal bacteria. | the development of multispecies biofilm models are needed to explain the interactions that take place in root canal biofilms during apical periodontitis. the aim of this study was to investigate the ability of 4 root canal bacteria to establish a multispecies biofilm community and to characterize the main structural, compositional, and physiological features of this community. | 2012 | 22341068 |
| in vitro evaluation of the probiotic potential of lactobacillus salivarius smxd51. | lactobacillus salivarius smxd51 was previously isolated from the cecum of a tunisian poultry and found to produce a bacteriocin-like substance highly active against the foodborne pathogen campylobacter jejuni. the aim of this study was to examine some probiotic properties of the strain: acid and bile tolerance, capacity of adhesion, stimulation of immune defences (il-6, il-8, il-10 and β-defensin 2), and modulation of the barrier integrity. the results showed that l. salivarius smxd51 can tolera ... | 2012 | 23122647 |
| competitive selection of lactic acid bacteria that persist in the human oral cavity. | lactic acid bacteria (lab) might offer opportunities as oral probiotics provided candidate strains persist in the mouth. after intake of a mixture of 69 lab, strains of lactobacillus fermentum and lactobacillus salivarius were especially recovered. coaggregation with other microbes is likely not a prerequisite for persistence since l. salivarius strongly coaggregated with typical oral cavity isolates, whereas l. fermentum failed to display this phenotype. | 2011 | 21965405 |
| lactobacillus salivarius and l. gasseri down-regulate aggregatibacter actinomycetemcomitans exotoxins expression. | beneficial microbes, such as lactobacilli establish a symbiosis with the host and confer health-associated effects, by limiting the growth of indigenous pathogens and challenging microbes introduced by altered foods. nevertheless, there is scarce information on the effects of beneficial microbes on the virulence properties of bacterial species associated with oral diseases, such as periodontitis. aggregatibacter actinomycetemcomitans is a gram-negative species highly implicated in the etiology o ... | 2014 | 24860281 |
| effect of bacterial cell-free supernatants on infectivity of norovirus surrogates. | bacterial metabolic products were evaluated for inhibitory effects on viral propagation in cell culture. cell-free supernatants (cfs) were prepared from growth of enterococcus faecalis atcc 19433, pseudomonas fluorescens atcc 13525, escherichia coli 08, staphylococcus epidermidis atcc 12228, bacillus subtilis 168, bacillus coagulans 185a, b. coagulans 7050, clostridium sporogenes pa3679, and a commercial probiotic mixture of lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium bif ... | 2014 | 24406013 |
| poultry farm hygiene: microbiological quality assessment of drinking water used in layer chickens managed under the battery cage and deep litter systems at three poultry farms in southwestern nigeria. | water troughs from deep litter and caged chicken water troughs (drinkers) fixed to each of the different 3-tier cages containing layer chickens in farms a, b and c were subjected to a 7-day study which involved the monitoring of poultry farm hygiene. drinkers were washed before filling with water on day 1. for days 3, 5 and 7 water was served without prior washing. the occurrence and characterization of the bacteria isolates were investigated and data obtained were analyzed and compared. for the ... | 2014 | 24783781 |
| different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens. | to evaluate the effects of different combinations of probiotics on performance, egg quality, and immune response of layer hens, a trial was carried out with 1,800 white feather layer hens of the lohmann variety. the experiment was conducted by using a completely randomized design with 9 treatments, 4 replicates, and 50 hens in each replicate. compared with the control group, group f, which added a composition of heat-inactivated lactobacillus salivarius(cb) and bacillus subtilis to the diets of ... | 2012 | 23091128 |
| co-administration of bacillus subtilis rjgp16 and lactobacillus salivarius b1 strongly enhances the intestinal mucosal immunity of piglets. | probiotics, including bacillus spp. and lactobacillus, are potential replacements for low dose in-feed antibiotics for pig. this study aimed to evaluate the effect of the co-administration of bacillus subtilis rjgp16 and lactobacillus salivarius b1 as potential probiotics to stimulate local immune responses. thirty two newborn piglets were divided into four groups and orally administrated with different combination of probiotics (none; rjgp16; b1; rjgp16 and b1) at the age of 0, 7 and 11 days. w ... | 2013 | 22901748 |
| effects of lactobacillus salivarius ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model. | probiotics have been suggested as a prophylactic measure in colon cancer. the aim of this study was to investigate the impact of lactobacillus salivarius ren (ren) in modulating colonic microbiota structure and colon cancer incidence in a rat model after injection with 1,2-dimethyl hydrazine (dmh). the results indicated that oral administration of ren could effectively suppress dmh-induced colonic carcinogenesis. a significant decrease in cancer incidence (87.5% to 25%) was detected in rats fed ... | 2015 | 26025172 |
| differential intestinal m-cell gene expression response to gut commensals. | different rates of bacterial translocation across the gut mucosa have been reported but few studies have examined translocation of commensals at the level of the gut epithelial microfold (m) cell. we used an in vitro m-cell model to quantify translocation and determine the transcriptional response of m cells to various commensal bacteria. the transport kinetics and gene expression profile of m cells in response to different bacterial strains, namely lactobacillus salivarius, escherichia coli and ... | 2012 | 22385384 |
| poultry blood from slaughterhouses: development of a biopreservation system to improve microbiological quality prior to transforming blood into by-products. | the aim of this study was to investigate the use of indigenous lactic acid bacteria (lab) with specific additives as a biopreservation system (bs) for poultry blood during its storage in slaughterhouses. the bs consisted of two lab (enterococcus faecalis dspv 008sa or lactobacillus salivarius dspv 032sa) with 4 additives (lactose 2 g/l, yeast extract 0.4 g/l, ammonium citrate 0.4 g/l and nacl 1 g/l). after 24 h storage at 30ºc, lower counts of enterobacteria, coliforms, pseudomonas spp. and stap ... | 2016 | 27352105 |
| probiotics in the prevention of eczema: a randomised controlled trial. | to evaluate a multistrain, high-dose probiotic in the prevention of eczema. | 2014 | 24947281 |
| assessment of probiotics supplementation via feed or water on the growth performance, intestinal morphology and microflora of chickens after experimental infection with eimeria acervulina, eimeria maxima and eimeria tenella. | in this study, the effect of probiotic supplementation via drinking water or feed on the performance of broiler chickens experimentally infected with sporulated oocysts of eimeria acervulina (5 × 10(4)), eimeria maxima and eimeria tenella (2 × 10(4) each one) at 14 days of age was evaluated. two hundred and forty 1-day-old ross 308 male chicks were separated into eight equal groups with three replicates. two of the groups, one infected with mixed eimeria oocysts and the other not, were given a b ... | 2014 | 24601749 |
| a randomized controlled trial to test the effect of multispecies probiotics on cognitive reactivity to sad mood. | recent insights into the role of the human microbiota in cognitive and affective functioning have led to the hypothesis that probiotic supplementation may act as an adjuvant strategy to ameliorate or prevent depression. | 2015 | 25862297 |
| antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens. | previous reports have shown that several probiotic strains can resist the antibacterial activity of bovine lactoferrin (blf), but the results are inconsistent. moreover, a portion of orally administered apo-blf is digested in vivo by pepsin to yield blf hydrolysate, which produces stronger antibacterial activity than that observed with apo-blf. however, whether blf hydrolysate affects the growth of probiotic strains is unclear. therefore, various probiotic strains in taiwan were collected and ev ... | 2013 | 23332852 |
| effect of probiotics on the treatment of children with atopic dermatitis. | atopic dermatitis, a chronic recurrent disease, is frequently encountered in clinical practice. in the last 30 years, the prevalence of atopic dermatitis has rapidly increased due to industrialization. therefore, there have been attempts in recent years to find new ways of treating and preventing atopic dermatitis. | 2012 | 22577270 |
| probiotics and refractory chronic spontaneous urticaria. | background. in chronic spontaneous urticaria (csu) first-line therapy with an antihistamine-based regimen may not achieve satisfactory control in patients. thus, a continuing need exists for effective and safe treatments for refractory csu. aim. to evaluate the clinical efficacy and safety of an intake of a combination of 2 probiotics (lactobacillus salivarius ls01 and bifidobacterium breve br03) in patients with csu who remain symptomatic despite concomitant h1-antihistamine therapy. methods. t ... | 2016 | 27608474 |
| a case of scalp rosacea treated with low dose doxycycline and probiotic therapy and literature review on therapeutic options. | rosacea is a common chronic inflammatory disorder showing a wide range of clinical features such as telangiectasia, erythema, papules, and pustules primarily involving the central part of face (forehead, cheeks and nose) although extra facial manifestation have been described. we describe a case of rosacea with predominant scalp involvement successfully treated with a 8-week-course of doxycycline 40 mg once a day and probiotic therapy twice a day (bifidobacterium breve br03, lactobacillus saliva ... | 2016 | 27087407 |
| anti-pathogenic and probiotic attributes of lactobacillus salivarius and lactobacillus plantarum strains isolated from feces of algerian infants and adults. | sixty-seven (67) lactic acid bacteria (lab) isolates belonging to lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. strains with the upmost auto-aggregation and cell surface hydrophobicity scores were identified by maldi-tof spectrometry and 16s rdna sequencing as lactobacillus plantarum (p25lb1 and p98lb1) and lactobacillus salivarius ... | 2017 | 28093322 |
| administration of bifidobacterium breve ps12929 and lactobacillus salivarius ps12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study. | the preterm infant gut has been described as immature and colonized by an aberrant microbiota. therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. the objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. in addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory b ... | 2015 | 25759843 |
| isolation and characterization of lactobacilli from human faeces and indigenous fermented foods for their potential application as probiotics. | this study was conducted to select lactobacillus strains from various sources on the basis of their probiotic attributes, such as acid and bile tolerance, binding to intestinal cells, and antimicrobial activity. twelve isolates were obtained from human and food sources and were evaluated against standard probiotic lactobacillus rhamnosus gg (lgg). isolates were also studied for their antibiotic susceptibility. isolate lactobacillus fermentum gpi-6 showed the best survival profile at 0.3% and 1% ... | 2016 | 26928011 |
| immunomodulatory effects of lactobacillus salivarius ls01 and bifidobacterium breve br03, alone and in combination, on peripheral blood mononuclear cells of allergic asthmatics. | the aim of this study was to evaluate probiotic characteristics of lactobacillus salivarius ls01 and bifidobacterium breve br03 alone and in combination and their immunomodulatory activity in asthmatic subjects. subjects affected by allergic asthma were recruited. initially, ls01 and br03 were analyzed for their growth compatibility by a broth compatibility assay. to study the antimicrobial activity of probiotic strains, an agar diffusion assay was performed. finally, cytokine production by peri ... | 2015 | 25749784 |
| probiotics reduce gut microbial translocation and improve adult atopic dermatitis. | it has been suggested that probiotics modulate atopic dermatitis (ad) progression, but no data are actually available on their mechanisms of action and on their ability to act as immunomodulators in this pathology. | 2012 | 22955355 |
| three novel candidate probiotic strains with prophylactic properties in a murine model of cow's milk allergy. | food allergies can have significant effects on morbidity and on quality of life. therefore, the development of efficient approaches to reduce the risk of developing food allergies is of considerable interest. the aim of this study was to identify and select probiotic strains with preventive properties against allergies using a combination of in vitro and in vivo approaches. to that end, 31 strains of bifidobacteria and lactic acid bacteria were screened for their immunomodulatory properties in t ... | 2016 | 26729723 |
| screening for lactic acid bacteria capable of inhibiting campylobacter jejuni in in vitro simulations of the broiler chicken caecal environment. | thermotolerant campylobacter spp., specifically campylobacter jejuni and campylobacter coli, are the most common bacterial causes of human gastroenteritis in developed countries. consumption of improperly prepared poultry products and cross contamination are among the main causes of human campylobacteriosis. the aim of this study was to identify lactic acid bacterial (lab) strains capable of inhibiting c. jejuni growth in initial in vitro trials ('spot-on-lawn' method), as well as in batch ferme ... | 2012 | 23234730 |
| use of the potential probiotic strain lactobacillus salivarius smxd51 to control campylobacter jejuni in broilers. | campylobacteriosis is the most frequently reported zoonotic disease in humans in the eu since 2005. as chicken meat is the main source of contamination, reducing the level of campylobacter in broiler chicken will lower the risk to consumers. the aim of this project was to evaluate the ability of lactobacillus salivarius smxd51 to control campylobacter jejuni in broilers and to investigate the mechanisms that could be involved. thirty broilers artificially contaminated with c. jejuni were treated ... | 2017 | 27432696 |
| lactic acid bacteria as a surface display platform for campylobacter jejuni antigens. | food poisoning and diarrheal diseases continue to pose serious health care and socioeconomic problems worldwide. campylobacter spp. is a very widespread cause of gastroenteritis. over the past decade there has been increasing interest in the use of lactic acid bacteria (lab) as mucosal delivery vehicles. they represent an attractive opportunity for vaccination in addition to vaccination with attenuated bacterial pathogens. | 2015 | 25662187 |
| purification and characterization of a new bacteriocin active against campylobacter produced by lactobacillus salivarius smxd51. | strain smxd51, isolated from chicken ceca and identified as lactobacillus salivarius, produced a component that inhibits the growth of gram-positive and gram-negative bacteria and especially campylobacter jejuni. the active peptide from the cell-free supernatant of lb. salivarius smxd51 was purified in three steps: (i) precipitation with 80% saturated ammonium sulfate, (ii) elution on a reversed phase spe upti-clean cartridge using different concentrations of acetonitrile, (iii) final purificati ... | 2012 | 22850384 |
| evaluating the efficacy of an avian-specific probiotic to reduce the colonization of campylobacter jejuni in broiler chickens. | campylobacteriosis is the most frequent zoonotic disease in humans worldwide, and the contaminated poultry meat by campylobacter jejuni can be considered one of the important sources of enteric infections in humans. the use of probiotics, which can help to improve the natural defense of animals against pathogenic bacteria, is an alternative and effective approach to antibiotic administration for livestock to reduce bacterial contamination. in vitro experiments showed that enterococcus faecium, p ... | 2012 | 22802174 |
| in vitro and in vivo effects of beneficial vaginal lactobacilli on pathogens responsible for urogenital tract infections. | the aim of this work was to evaluate the effects of beneficial human vaginal lactobacilli (lb) on urogenital pathogens through in vitro and in vivo experiments. co-aggregative and antimicrobial properties between five vaginal lb strains and urogenital pathogens or potential pathogens (streptococcus agalactiae, staphylococcus aureus and candida albicans strains) were assayed. also, associative cultures of lb strains and s. agalactiae were performed and bacterial growth, ph, lactic acid and hydrog ... | 2014 | 24523160 |
| oral administration of a select mixture of bacillus probiotics affects the gut microbiota and goblet cell function following escherichia coli challenge in newly weaned pigs of genotype muc4 that are supposed to be enterotoxigenic e. coli f4ab/ac receptor negative. | structural disruption of the gut microbiota and impaired goblet cell function are collateral etiologic factors in enteric diseases. low, moderate, or high doses of a bacillus licheniformis-b. subtilis mixture (bls mix) were orally administered to piglets of genotype muc4 that are supposed to be f4-expressing enterotoxigenic escherichia coli strain (f4(+) etec) f4ab/ac receptor negative (i.e., muc4-resistant piglets) for 1 week before f4(+) etec challenge. the luminal contents were collected from ... | 2017 | 27881419 |
| 5-fluorouracil sensitivity varies among oral micro-organisms. | 5-fluorouracil (5-fu), a commonly used chemotherapeutic agent, often causes oral mucositis, an inflammation and ulceration of the oral mucosa. micro-organisms in the oral cavity are thought to play an important role in the aggravation and severity of mucositis, but the mechanisms behind this remain unclear. although 5-fu has been shown to elicit antibacterial effects at high concentrations (>100 µm), its antibacterial effect at physiologically relevant concentrations in the oral cavity is unknow ... | 2016 | 27296270 |
| in vitro inhibition of eimeria tenella sporozoite invasion into host cells by probiotics. | the aim was to study the effects of probiotics isolated from the intestinal tract of livestock animals on eimeria tenella invasion into madin-darby bovine kidney (mdbk) cells in vitro. e. tenella sporozoites were purified and labeled with 5(6)-carboxyfluorescein diacetate n-succinimidyl ester before seeding on cell cultures, and invasion was evaluated by fluorescence microscopy. two protocols (a and b) were used. in protocol a, enterococcus faecium # 589 or lactobacillus salivarius subsp. saliva ... | 2016 | 27809987 |
| antimicrobial activity of lactobacillus strains of chicken origin against bacterial pathogenss. | this study was conducted to identify and evaluate the antimicrobial activity of some lactobacillus isolates of chicken origin. among 90 isolates 14 lactobacillus species were distinguished using maldi-tof mass spectrometry and 16s-ardra. the dominant species was l. salivarius (34.4%), followed by l. johnsonii (23.3%), l. crispatus (13.3%) and l. reuteri (11.1%). all lactobacilli were screened for antimicrobial activity against wild-type strains of salmonella enterica, escherichia coli, and clost ... | 2016 | 27762430 |
| crystal structure of bile salt hydrolase from lactobacillus salivarius. | bile salt hydrolase (bsh) is a gut-bacterial enzyme that negatively influences host fat digestion and energy harvesting. the bsh enzyme activity functions as a gateway reaction in the small intestine by the deconjugation of glycine-conjugated or taurine-conjugated bile acids. extensive gut-microbiota studies have suggested that bsh is a key mechanistic microbiome target for the development of novel non-antibiotic food additives to improve animal feed production and for the design of new measures ... | 2016 | 27139829 |
| screening of lactobacillus strains of domestic goose origin against bacterial poultry pathogens for use as probiotics. | lactobacilli are natural inhabitants of human and animal mucous membranes, including the avian gastrointestinal tract. recently, increasing attention has been given to their probiotic, health-promoting capacities, among which their antagonistic potential against pathogens plays a key role. a study was conducted to evaluate probiotic properties of lactobacillus strains isolated from feces or cloacae of domestic geese. among the 104 examined isolates, previously identified to the species level by ... | 2014 | 25104766 |
| presence of erythromycin and tetracycline resistance genes in lactic acid bacteria from fermented foods of indian origin. | lactic acid bacteria (lab) resistant to erythromycin were isolated from different food samples on selective media. the isolates were identified as enterococcus durans, enterococcus faecium, enterococcus lactis, enterococcus casseliflavus, lactobacillus salivarius, lactobacillus reuteri, lactobacillus plantarum, lactobacillus fermentum, pediococcus pentosaceus and leuconostoc mesenteroides. of the total 60 isolates, 88 % harbored the ermb gene. the efflux gene msra was identified in e. faecium, e ... | 2012 | 22644346 |
| colonisation of poultry by salmonella enteritidis s1400 is reduced by combined administration of lactobacillus salivarius 59 and enterococcus faecium pxn-33. | salmonella enteritidis remains a significant issue within the poultry industry and one potential solution is to use probiotic bacteria to prevent salmonella colonisation through competitive exclusion (ce). we demonstrate that combined administration of lactobacillus salivarius 59 and enterococcus faecium pxn33 were effective competitive excluders of salmonella enteritidis s1400 in poultry. two models were developed to evaluate the efficacy of probiotic where birds received salmonella enteritidis ... | 2017 | 28110775 |
| using in vitro immunomodulatory properties of lactic acid bacteria for selection of probiotics against salmonella infection in broiler chicks. | poultry is known to be a major reservoir of salmonella. the use of lactic acid bacteria has become one of successful strategies to control salmonella in poultry. the purpose of this study was to select lactic acid bacteria strains by their in vitro immunomodulatory properties for potential use as probiotics against salmonella infection in broiler chicks. among 101 isolated lactic acid bacteria strains, 13 strains effectively survived under acidic (ph 2.5) and bile salt (ranging from 0.1% to 1.0% ... | 2016 | 26799658 |
| potential probiotic lactic acid bacteria of human origin induce antiproliferation of colon cancer cells via synergic actions in adhesion to cancer cells and short-chain fatty acid bioproduction. | the activities and modes of probiotic action of lactic acid bacteria isolated from infant feces were investigated for alternative application in the prevention and biotherapy of colon cancer. from a total of 81 isolates of gram-positive rod and cocci bacteria obtained from healthy infants, only 15 isolates had the probiotic criteria which included growth inhibition against eight food-borne pathogens, no blood hemolysis, and tolerance to gastrointestinal tract properties such as ph 2.5 and 0.3 % ... | 2013 | 23239414 |
| isolation and characterization of bacteriocin-producing bacteria from the intestinal microbiota of elderly irish subjects. | to isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects. | 2013 | 23181509 |
| dietary inclusion of feathers affects intestinal microbiota and microbial metabolites in growing leghorn-type chickens. | feather pecking in laying hens is a serious behavioral problem that is often associated with feather eating. the intake of feathers may influence the gut microbiota and its metabolism. the aim of this study was to determine the effect of 2 different diets, with or without 5% ground feathers, on the gut microbiota and the resulting microbial fermentation products and to identify keratin-degrading bacteria in chicken digesta. one-day-old lohmann-selected leghorn chicks were divided into 3 feeding ... | 2012 | 22700493 |