| purification and characterization of a surface protein from lactobacillus fermentum 104r that binds to porcine small intestinal mucus and gastric mucin. | an adhesion-promoting protein involved in the binding of lactobacillus fermentum strain 104r to small intestinal mucus from piglets and to partially purified gastric mucin was isolated and characterized. spent culture supernatant fluid and bacterial cell wall extracts were fractionated by ammonium sulfate precipitation and gel filtration. the active fraction was purified by affinity chromatography. the adhesion-promoting protein was detected in the fractions by adhesion inhibition and dot blot a ... | 2002 | 11976105 |
| lactobacillus fermentum rc-14 inhibits staphylococcus aureus infection of surgical implants in rats. | staphylococcus aureus is a common cause of community and hospital-acquired infections. moreover, the clinical impact of s. aureus is on the rise because of the global increase in the incidence of multidrug-resistant strains and its growing prevalence as a major cause of surgical infections. as a result, there is a pressing need to identify new antistaphylococcal agents and preventative strategies that will help in the management of these types of infections. this report describes the successful ... | 2002 | 12001060 |
| mononuclear cell response to enterobacteria and gram-positive cell walls of normal intestinal microbiota in early rheumatoid arthritis and other inflammatory arthritides. | to study whether enterobacteria and gram-positive bacterial cell walls (bcw) derivedfrom normal intestinal microbiota are involved in the etiopathogenesis of early rheumatoid arthritis (ra). | 2002 | 12051398 |
| expression of a streptococcal glucosyltransferase as a fusion to a solute-binding protein in lactobacillus fermentum br11. | bspa is a non-covalently anchored cystine-binding protein from lactobacillus fermentum br11. it has previously been used to present antigens derived from infectious organisms on the l. fermentum br11 cell surface. in this study, the capacity of bspa to present a very large polypeptide was tested. a temperature sensitive plasmid was constructed that encodes a 175-kda chimeric protein consisting of a fusion between bspa and an n-terminally truncated derivative of the streptococcus salivarius atcc ... | 2002 | 12052553 |
| characterization of a cryptic plasmid from lactobacillus fermentum kc5b and its use for constructing a stable lactobacillus cloning vector. | lactobacillus fermentum kc5b, a strain originally isolated from the human vagina, contains a cryptic plasmid pkc5b. the sequence and genetic organization of the 4392-bp plasmid were determined. it contains two convergently oriented replicons, which are homologous to each other and to the stable replicon of the enterococcus faecium plasmid pmbb1. the two replicons of pkc5b were used either individually or together to construct lactobacillus-escherichia coli shuttle plasmids. only the plasmid psp1 ... | 2002 | 12151233 |
| effects of asparaginase and polyphenol oxidase on adhesive characteristics of microorganisms. | we studied the effects of polyphenol oxidase and asparaginase on microorganism adhesion to buccal epithelial cells. these enzymes reduced adhesion of pathogenic microorganisms (uropathogenic and escherichia coli, salmonella enteritidis, entamoeba spp., influenza virus, candida albicans, streptococcus spp.) and had virtually no effect on adhesive characteristics of probiotic variants of escherichia coli and lactobacillus fermentum. | 2002 | 12170308 |
| heat resistance of juice spoilage microorganisms. | the heat resistance of various yeasts (saccharomyces cerevisiae, rhodotorula mucilaginosa, torulaspora delbrueckii, and zygosaccharomyces rouxii), molds (penicillium citrinum, penicillium roquefortii, and aspergillus niger), and lactic acid bacteria (lactobacillus fermentum and lactobacillus plantarum) obtained from spoiled acid or acidified food products was determined in 0.1 m citrate buffer at ph values of 3.0, 3.5, and 4.0. s. cerevisiae was the most heat resistant of the microorganisms in c ... | 2002 | 12182479 |
| cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. | the objective of this study was to evaluate the effect of human gut-derived lactic acid bacteria and bifidobacteria on cholesterol levels in vitro. continuous cultures inoculated with fecal material from healthy human volunteers with media supplemented with cholesterol and bile acids were used to enrich for potential cholesterol assimilators among the indigenous bacterial populations. seven potential probiotics were found: lactobacillus fermentum strains f53 and kc5b, bifidobacterium infantis at ... | 2002 | 12200334 |
| lactobacillus reuteri dsm 20016: purification and characterization of a cystathionine gamma-lyase and use as adjunct starter in cheesemaking. | a homo-tetrameric approximately 160-kda cystathionine gamma-lyase was purified to homogeneity from lactobacillus reuteri dsm 20016 by four chromatographic steps. the activity was pyridoxal-5'-phosphate dependent and the enzyme catalyzed the alpha,gamma-elimination reaction of l-cystathionine, producing l-cysteine, ammonia and alpha-ketobutyrate. the enzyme was active towards a range of amino acids and amino acid derivatives, including methionine. the ph and temperature optima were found to be 8. ... | 2002 | 12222803 |
| fermentation by lactobacillus fermentum ogi e1 of different combinations of carbohydrates occurring naturally in cereals: consequences on growth energetics and alpha-amylase production. | glucose, fructose, sucrose and starch are naturally present in cereals. fermentation of different combinations of these carbohydrates by lactobacillus fermentum ogi e1, a sourdough heterofermentative lactobacillus, was investigated to determine effects on fermentation kinetics, growth energetics and alpha-amylase production. irrespective of the substrate combination, the strain was able to simultaneously produce alpha-amylase and consume starch, glucose, fructose and sucrose. in mixtures of star ... | 2003 | 12381402 |
| microbiological and fermentation characteristics of togwa, a tanzanian fermented food. | selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ... | 2003 | 12423921 |
| study of starch fermentation at low ph by lactobacillus fermentum ogi e1 reveals uncoupling between growth and alpha-amylase production at ph 4.0. | lactobacillus fermentum ogi e1 is an amylolytic heterofermentative lactic acid bacterium previously isolated from ogi, a benin maize sourdough. in the present study, the effect of different ph between 3.5 and 6.0 on starch fermentation products and alpha-amylase production was investigated. whereas a ph of 5.0 was optimum for specific growth rate and lactic acid production, growth was only slightly affected at suboptimal ph of 4.0 and 6.0. over a ph range of 6.0 to 3.5, yields of product formati ... | 2003 | 12430774 |
| isolation, characterization and inhibition by acarbose of the alpha-amylase from lactobacillus fermentum: comparison with lb. manihotivorans and lb. plantarum amylases. | extracellular alpha-amylase from lactobacillus fermentum (fermenta) was purified by glycogen precipitation and ion exchange chromatography. the purification was approximately 28-fold with a 27% yield. the fermenta molecular mass (106,000 da) is in the same range as the ones determined for l. amylovorus (amyloa), l. plantarum (plantaa) and l. manihotivorans (manihoa) alpha-amylases. the amino acid composition of fermenta differs from the other lactobacilli considered here, but however, indicates ... | 2002 | 12431403 |
| vaginal bacterial flora activates rat peritoneal mast cells. | sixteen strains of physiological and pathological vaginal bacteria were tested for their ability to secrete histamine from rat peritoneal mast cells in vitro. we noticed that mycoplasma hominis-induced histamine release was very high (up to 53.6%). the stimulation of rat mast cells with staphylococccus cohnii, staphylococcus coagulase(-) (two strains), ureaplasma urealyticum, peptostreptococcus spp., bacteroides capillosus, staphylococcus aureus and streptococcus agalactiae resulted in lower but ... | 2002 | 12575924 |
| volatile compounds produced by lactobacillus fermentum, saccharomyces cerevisiae and candida krusei in single starter culture fermentations of ghanaian maize dough. | to identify and compare the volatile compounds associated with maize dough samples prepared by spontaneous fermentation and by the use of added starter cultures in ghana. | 2003 | 12588555 |
| oral use of lactobacillus rhamnosus gr-1 and l. fermentum rc-14 significantly alters vaginal flora: randomized, placebo-controlled trial in 64 healthy women. | urogenital infections afflict an estimated one billion people each year. the size of this problem and the increased prevalence of multi-drug resistant pathogens make it imperative that alternative remedies be found. a randomized, placebo-controlled trial of 64 healthy women given daily oral capsules of lactobacillus rhamnosus gr-1 and lactobacillus fermentum rc-14 for 60 days showed no adverse effects. microscopy analysis showed restoration from asymptomatic bacterial vaginosis microflora to a n ... | 2003 | 12628548 |
| isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of escherichia coli o157:h7 in cattle. | lactic acid bacteria (lab) were selected on the basis of characteristics indicating that they would be good candidates for a competitive exclusion product (cep) that would inhibit escherichia coli o157:h7 in the intestinal tract of live cattle. fecal samples from cattle that were culture negative for e. coli o157:h7 were collected. lab were isolated from cattle feces by repeated plating on deman rogosa sharpe agar and lactobacillus selection agar. six hundred eighty-six pure colonies were isolat ... | 2003 | 12636285 |
| lactobacillus ingluviei sp. nov., isolated from the intestinal tract of pigeons. | lactic acid bacteria were isolated from the crop and intestines of pigeons. one group of strains, showing similar genomic patterns after screening with trna intergenic spacer pcr, could not be identified to the species level. sequencing of the 16s rrna gene of one representative strain revealed about 96% similarity to sequences from lactobacillus fermentum and lactobacillus mucosae. determination of the dna base composition, dna-dna hybridization experiments, sds-page of whole-cell proteins and ... | 2003 | 12656164 |
| lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces. | five strains of thermotolerant lactic acid bacteria (g 12, g 22, g 35t, g 43 and g 44) isolated from chicken faeces were characterized taxonomically. the strains were facultatively anaerobic, gram-positive, catalase-negative, non-motile, non-spore-forming rods. they were heterofermentative lactobacilli that produced dl-lactic acid. growth of the strains occurred at 45 degrees c but not at 15 degrees c. the optimum temperature for growth was 42 degrees c, as determined from the specific growth ra ... | 2003 | 12656183 |
| metabolic engineering of lactobacillus fermentum for production of mannitol and pure l-lactic acid or pyruvate. | for production of mannitol in combination with pure l-lactic acid or pyruvate, the d- and l-lactate dehydrogenase genes (ldhd and ldhl) of a mannitol-producing lactobacillus fermentum strain were cloned and stepwise inactivated. for inactivation of both ldh genes by a gene replacement technique, deletion constructs removing a 0.4-kb fragment from the promoter and the 5' end region of the ldh genes were used. the first inactivation mutant, designated l. fermentum grl1030, carried the deletion in ... | 2003 | 12673764 |
| lectin-like binding of lactobacilli considered for their use in probiotical preparations for animal use. | three gut lactobacilli from piglets (lactobacillus plantarum l 5, lactobacillus paracasei l 81, lactobacillus fermentum l 670) and lactobacillus casei subsp. pseudoplantarum l.c.) from a calf were examined by microtitre plate binding assay for their lectin-like binding activity after their cultivation on rogosa agar and in mrs broth. three ecm (extracellular matrix) molecules (fetuin, porcine fibronectin and porcine mucin) were selected for this assay. additionally, the effect of heparin on the ... | 2003 | 12680274 |
| evidence for chromosomal determination of fungicidal activity in strains of lactobacillus brevis and lactobacillus fermentum isolated from fermented foods. | the genetic basis of the fungicidal activity of strains of lactobacillus brevis and l. fermentum isolated from indigenous fermented foods was determined. a 5.5-kb plasmid was isolated from l. brevis while l. fermentum was found to harbor no plasmid. plasmid curing indicated no correlation between the plasmid and the fungicidal activity of the lactobacillus species. the fungicidal activity of the isolated organisms can be supposed to be mediated by the chromosome. no antibiotic resistance genetic ... | 2003 | 12744077 |
| use of starter cultures of lactic acid bacteria and yeasts in the preparation of togwa, a tanzanian fermented food. | starter cultures of lactic acid bacteria (lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum and pediococcus pentosaceus) and yeasts (candida pelliculosa, candida tropicalis, issatchenkia orientalis and saccharomyes cerevisiae) isolated from native togwa were tested singly or in combination for their ability to ferment maize-sorghum gruel to produce togwa. all species of bacteria showed an ability to ferment the gruel as judged by lowering the ph fr ... | 2003 | 12745235 |
| effect of lactobacillus buchneri, lactobacillus fermentum, leuconostoc mesenteroides inoculants, or a chemical additive on the fermentation, aerobic stability, and nutritive value of crimped wheat grains. | the preservation of crimped wheat grains by three bacterial inoculants or a chemical additive was compared. crimped wheat grain [56.8 g dry matter (dm)/kg] was conserved in 1.75-kg plastic bag, mini-silos without treatment, with 4l/tonne of crimpstore (cs; an additive containing a mixture of ammonium formate, propionate, ethyl benzoate, and benzoate, sas kelvin cave, ltd., uk) or 1 x 10(5) cfu/g of each of three inoculant additives containing lactobacillus fermentum (a), leuconostoc mesenteroide ... | 2003 | 12778589 |
| structural and ultrastructural studies of the urinary tract of mice inoculated with lactobacillus fermentum. | to assess, using structural and ultrastructural studies of the urinary tract, the effects of the intraurethral inoculation of lactobacilli (probiotic treatment) as lactobacilli are the predominant micro-organisms of the urogenital tract of humans, monkeys and mice. | 2003 | 12780852 |
| proteolytic activities in togwa, a tanzanian fermented food. | proteolytic activities were investigated in sorghum-based togwa prepared by natural fermentation and using starter cultures previously isolated from the native product, i.e., lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum, pediococcus pentosaceus, and issatchenkia orientalis in coculture with either l. brevis or l. plantarum. both proteinase and aminopeptidase activities were substantially higher in naturally fermented togwa than in those with s ... | 2003 | 12781948 |
| identification of lactic acid bacteria isolated from human vaginal secretions. | a total of 57 lactic acid bacteria were isolated from the vaginal secretions of 259 patients. of these strains, 37 were isolated from patients attending pre-natal clinics and the remaining strains from patients attending post-natal clinics. the strains were identified by using simple physiological and biochemical tests and their phenotypic relatedness determined by numerical analysis of total soluble cell protein patterns. the genotypic relatedness of representative strains selected from each of ... | 2003 | 12785305 |
| generation of food-grade lactococcal starters which produce the lantibiotics lacticin 3147 and lacticin 481. | transconjugant lactococcal starters which produce both lantibiotics lacticin 3147 and lacticin 481 were generated via conjugation of large bacteriocin-encoding plasmids. a representative of one of the resultant strains proved more effective at killing lactobacillus fermentum and inhibiting the growth of listeria monocytogenes lo28h than either of the single bacteriocin-producing parental strains, demonstrating the potential of these transconjugants as protection cultures for food safety applicat ... | 2003 | 12788782 |
| decrease in ovalbumin specific ige of mice serum after oral uptake of lactic acid bacteria. | different kinds of lactobacilli and bifidobacteria fermented milk were fed to ovalbumin-specific ige-elevated mice for 3 days, and after the final administration, changes in the ovalbumin-specific ige values for each sample were compared to the value for non-fermented milk. seven of the lactobacillus-fermented milks caused a significant decrease in the serum ovalbumin-specific ige levels. above all, lactobacillus acidophilus l92, lactobacillus acidophilus cp1613, and lactobacillus fermentum cp34 ... | 2003 | 12834270 |
| the microbial ecology of cocoa bean fermentations in indonesia. | cocoa beans are the principal raw material of chocolate manufacture. the beans are subject to a microbial fermentation as the first stage in chocolate production. the microbial ecology of bean fermentation (forastero and trinitario cultivars) was investigated at three commercial fermentaries in east java, indonesia by determining the populations of individual species at 12-h intervals throughout the process. the first 2-3 days of fermentation were characterised by the successional growth of vari ... | 2003 | 12892924 |
| an in vitro study of the probiotic potential of a bile-salt-hydrolyzing lactobacillus fermentum strain, and determination of its cholesterol-lowering properties. | this study evaluated the use of a bile-salt-hydrolyzing lactobacillus fermentum strain as a probiotic with potential hypocholesterolemic properties. the effect of l. fermentum on representative microbial populations and overall metabolic activity of the human intestinal microbiota was investigated using a three-stage continuous culture system. also, the use of galactooligosaccharides as a prebiotic to enhance growth and/or activity of the lactobacillus strain was evaluated. administration of l. ... | 2003 | 12902267 |
| antioxidative probiotic fermented goats' milk decreases oxidative stress-mediated atherogenicity in human subjects. | the increasing interest in a healthy diet is stimulating innovative development of novel scientific products in the food industry. the viable lactic acid bacteria in fermented milk products, such as yoghurt, have been associated with increased lactose tolerance, a well-balanced intestinal microflora, antimicrobial activity, stimulation of the immune system and antitumoural, anticholesterolaemic and antioxidative properties in human subjects. recently, we have studied a human lactobacillus spp. s ... | 2003 | 12908907 |
| thiamine sparing action of ascorbic acid on lactobacillus fermenti 36. | | 1953 | 13055949 |
| nutritional studies with lactobacillus fermenti. | | 1953 | 13069440 |
| species differentiation of oral lactobacilli from man including description of lactobacillus salivarius nov spec and lactobacillus cellobiosus nov spec. | | 1953 | 13069442 |
| [microbiological determination of vitamin b1 with the help of lactobacillus fermenti]. | | 1956 | 13373834 |
| improved medium for assay of thiamine with lactobacillus fermenti. | | 1957 | 13459314 |
| effect of oleic acid on the response of lactobacillus fermenti to thiamin and its moieties. | | 1958 | 13538925 |
| glutamic acid racemase from lactobacillus fermenti. purification and properties. | | 1961 | 13775143 |
| activation of the disulfide forms of thiamine and its phosphates as growth factors for lactobacillus fermenti. | | 1960 | 13796439 |
| [on the growth effects of thiamine disulfide on lactobacillus fermenti 36 in the agar plate test]. | | 1959 | 13796441 |
| the effect of s-benzoyl derivatives of thiamine on the growth of lactobacillus fermenti 36. | | 1962 | 14031536 |
| thiamine activity of thiamine propyl disulfide on the growth of lactobacillus fermenti 36. | | 1964 | 14217467 |
| influence of sugars on the growth of lactobacillus fermenti 36. | | 1965 | 14341424 |
| stimulatory effect of calcium on growth of lactobacillus fermenti. | | 1955 | 14357401 |
| plate assay of thiamine. ii. using lactobacillus fermenti. | | 1959 | 14403841 |
| sensitization of staphylococcus aureus and escherichia coli to antibiotics by the sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone. | the sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone were investigated for their abilities to enhance bacterial permeability and susceptibility to exogenous antimicrobial compounds. initially, it was observed by flow cytometry that these sesquiterpenoids promoted the intracellular accumulation of the membrane-impermeant nucleic acid stain ethidium bromide by live cells of lactobacillus fermentum, suggesting that enhanced permeability resulted from disruption of the cytoplasmic membr ... | 2003 | 14506058 |
| peptide surface display and secretion using two lpxtg-containing surface proteins from lactobacillus fermentum br11. | a locus encoding two repetitive proteins that have lpxtg cell wall anchoring signals from lactobacillus fermentum br11 has been identified by using an antiserum raised against whole l. fermentum br11 cells. the first protein, rlp, is similar to the rib surface protein from streptococcus agalactiae, while the other protein, mlp, is similar to the mucus binding protein mub from lactobacillus reuteri. it was shown that multiple copies of mlp exist in the genome of l. fermentum br11. regions of rlp, ... | 2003 | 14532035 |
| cystine uptake prevents production of hydrogen peroxide by lactobacillus fermentum br11. | bspa is an abundant surface protein from lactobacillus fermentum br11, and is required for normal cystine uptake. in previous studies, a mutant strain deficient in bspa (l. fermentum png201) was found to be sensitive to oxidative stress. in this study, the biochemical basis for this was explored. it was found that under aerobic batch culture conditions in de mann-rogosa-sharpe medium, both l. fermentum br11 and png201 entered stationary phase due to hydrogen peroxide accumulation. however, this ... | 2003 | 14568153 |
| adhesion of probiotic lactobacilli to chick intestinal mucus. | in the present work, interactions between three lactobacillus strains (lactobacillus fermentum crl1015, lactobacillus animalis crl1014, and lactobacillus fermentum crl1016) and chicken small intestinal mucus were determined. three lactobacilli isolated from chicken and selected by their potentially probiotic properties were able to grow in mucus preparations. three peaks from gel filtration chromatography of intestinal mucus were obtained. the adhesion to three mucus fractions (i, ii, and iii), ... | 2003 | 14569288 |
| antimicrobial interactions of microbial species involved in the fermentation of cassava dough into agbelima with particular reference to the inhibitory effect of lactic acid bacteria on enteric pathogens. | lactic acid bacteria, bacillus species and yeasts are involved in the fermentation of cassava dough into agbelima. microbial interactions within and between these groups of microorganisms were investigated in addition to the survival of five enteric pathogens inoculated into agbelima under various conditions. nine out of 10 cultures of lactic acid bacteria isolated at the end of agbelima fermentation showed inhibitory effect against 10 cultures of lactic acid bacteria isolated at the start of fe ... | 2003 | 14580972 |
| sequence and genetic organization of the 19.3-kb erythromycin- and dalfopristin-resistance plasmid plme300 from lactobacillus fermentum rot1. | lactobacillus fermentum rot1 was isolated from a raw milk dairy product. it is resistant to novobiocin, tetracycline, erythromycin and dalfopristin. a chromosomal tetracycline-resistance determinant was identified as tetm. a 19,398-bp plasmid (plme300), present in several erythromycin-resistant strains of lb. fermentum, was isolated from strain rot1 and completely sequenced. based on putative open reading frames, plme300 contains at least four different functional regions. in region i, orf1 show ... | 2003 | 14597008 |
| [influence lactobacilli on the functional activity of macrophages and delayed hypersensitivity reaction in mice]. | the influence of the genus lactobacillus strains on the macrophages' functional activity and intensity of the delayed hypersensitivity reaction in mice has been studied. it has been shown that lactobacilli take positive effect on the cell-mediated mechanisms of the organisms' non-specific resistance and do not intensify the proceeding of specific immune reaction. the strain lactobacillus fermentum atcc 14931 is promising as the basis for the immunomodulatory probiotic preparation. | 2003 | 14618783 |
| screening for yeast with antibacterial properties from an ethanol distillery. | a general screening for the expression of antibacterial activity and non-flocculating type of yeast strains from must and fermented broth of alcohol distilleries was performed. from 60 strains only saccharomyces sp. m26 presented a inhibitory halo in lactobacillus fermentum culture and significant reduction in the culture turbidity (71%) and specific growth rate (56%) when compared to the control. freezing did not affect the antibacterial activity of the saccharomyces sp. m26 extract and heating ... | 2004 | 14643979 |
| assessment of in-line near-infrared spectroscopy for continuous monitoring of fermentation processes. | the application of nir in-line to monitor and control fermentation processes was investigated. determination of biomass, glucose, and lactic and acetic acids during fermentations of staphylococcus xylosus es13 was performed by an interactance fiber optic probe immersed into the culture broth and connected to a nir instrument. partial least squares regression (plsr) calibration models of second derivative nir spectra in the 700-1800 nm region gave satisfactory predictive models for all parameters ... | 2003 | 14656161 |
| fermentation of vigna sinensis var. carilla flours by natural microflora and lactobacillus species. | natural fermentation and an inoculum containing 10% (vol/vol) lactobacillus fermentum or lactobacillus plantarum were used to obtain fermented flours from vigna sinensis l. var. carilla seeds that had been washed with distilled water and dried at 55 degrees c for 24 h. to optimize the fermentation parameters (lactic acid bacterium level, bean flour concentration, and fermentation time), several small-scale fermentation processes were carried out. on the basis of the results obtained, fermentor-s ... | 2003 | 14672230 |
| microbiological characterization and probiotic potential of koko and koko sour water, african spontaneously fermented millet porridge and drink. | to identify and examine the diversity of predominant lactic acid bacteria (lab) in koko and koko sour water (ksw) from different ghanaian production sites with regard to pattern of fermentation (api 50 chl), genotype, antimicrobial activity, and resistance to low ph and bile salts. | 2004 | 14723700 |
| effect of ph on lactobacillus fermentum growth, raffinose removal, alpha-galactosidase activity and fermentation products. | in this study, the behaviour of lactobacillus fermentum crl 722 and crl 251 were evaluated under different ph conditions (ph 6.0, 5.5, 5.0, 4.5) and without ph control. growth was similar under all conditions assayed except at ph 4.5. these microorganisms were able to eliminate raffinose, a nondigestible alpha-oligosaccharide (ndo) found in soy products, showing a consumption rate of 0.25 g l(-1) h(-1) (ph 6.0-5.0). the removal of raffinose was due to the high alpha-galactosidase (alpha-gal) act ... | 2004 | 14727095 |
| microbiological assay method for thiamine using lactobacillus fermenti 36 as test organism. | | 1951 | 14911916 |
| characterisation of the microbiota of rice sourdoughs and description of lactobacillus spicheri sp. nov. | the microbiota of two industrially processed rice sourdoughs was characterised by bacteriological culture in combination with pcr-denaturing gradient gel electrophoresis (dgge) and 16s/28s rdna sequence analysis. rice sourdough i was continuously propagated for several years by back-slopping every week, whereas sourdough ii was processed by using a commercial starter culture and back-slopping daily for three days. in rice sourdough ii candida krusei and saccharomyces cerevisiae as well as lactob ... | 2004 | 15046303 |
| isolation and characterization of lactobacillus species inhibiting the formation of streptococcus mutans biofilm. | oral bacterium s11 was isolated from the saliva of young children without dental caries and with little or no visible supragingival plaque. the s11 strain showed 99.5% similarity with lactobacillus fermentum, and was identified on the basis of biochemical characteristics and a 16s rdna sequence. s11 strain and its culture supernatant significantly inhibited the formation of the insoluble glucan produced by streptococcus mutans ingbritt. s11 did not affect the multiplication of s. mutans ingbritt ... | 2004 | 15107076 |
| indigenous raw milk microbiota influences the bacterial development in traditional cheese from an alpine natural park. | nostrano di primiero is a 6-month ripened cheese produced from raw milk collected in the paneveggio-pale di san martino natural park area in the italian dolomites. in summer, this cheese is made using milk collected from two different areas, passo rolle and vanoi, in the paneveggio natural park. during the experiment, the milk from the two areas was separately processed, and cheeses were made in the same cheese factory using the same technological process. the microbiota of raw milk and cheeses ... | 2004 | 15109791 |
| reclassification of lactobacillus cellobiosus rogosa et al. 1953 as a later synonym of lactobacillus fermentum beijerinck 1901. | the name lactobacillus cellobiosus is validly published, but the species is often neglected in taxonomic studies, due to its high similarity to lactobacillus fermentum. in the present paper, literature data concerning the two species were reviewed. phylogenetic placement of l. cellobiosus was obtained based on 16s rdna sequences, and genetic similarity was further investigated by comparing partial reca gene sequences for the type strains of l. cellobiosus and l. fermentum. based on the high iden ... | 2004 | 15143028 |
| identification and characterization of the novel lysm domain-containing surface protein sep from lactobacillus fermentum br11 and its use as a peptide fusion partner in lactobacillus and lactococcus. | examination of supernatant fractions from broth cultures of lactobacillus fermentum br11 revealed the presence of a number of proteins, including a 27-kda protein termed sep. the amino-terminal sequence of sep was determined, and the gene encoding it was cloned and sequenced. sep is a 205-amino-acid protein and contains a 30-amino-acid secretion signal and has overall homology (between 39 and 92% identity) with similarly sized proteins of lactobacillus reuteri, enterococcus faecium, streptococcu ... | 2004 | 15184172 |
| [expression vector plf22 for the lactic acid bacteria]. | the construction of the expression vector plf22 for lactic acid bacteria is described. the vector contains a replicon of the cryptic plasmid plf1311 from lactobacillus fermentum and a multiple cloning site of the lacz' gene integrated with the plasmid rep operon. such a construction of the vector provides for the constitutive transcription of the cloned sequences lacking the terminators of transcription in all the strains that maintain the replication of the vector. the vector is suitable for a ... | 2004 | 15198033 |
| isolation, identification and characterisation of the dominant microorganisms of kule naoto: the maasai traditional fermented milk in kenya. | from 22 samples of kule naoto, the traditional fermented milk products of the maasai in kenya, 300 lactic acid bacterial strains were isolated and phenotypically characterised by their ability to ferment different carbohydrates and by additional biochemical tests. lactic acid bacteria (lab), especially the genus lactobacillus, followed by enterococcus, lactococcus and leuconostoc, dominated the microflora of these samples. the major lactobacillus species was lactobacillus plantarum (60%), with a ... | 2004 | 15246238 |
| nucleic acid-based diagnosis of bacterial vaginosis and improved management using probiotic lactobacilli. | bacterial vaginosis (bv) is a common condition in women that represents an imbalance of the vaginal microflora, lactobacilli depletion, and excess growth of mainly anaerobic gram-negative pathogens. diagnosis is made using a series of tests or a gram stain of a vaginal smear. treatment with antibiotics is quite effective, but recurrences are common. a study of 55 vaginal samples from 11 postmenopausal women showed the presence of bv by the gram stain-based nugent scoring system, and polymerase c ... | 2004 | 15298771 |
| a new probiotic cheese with antioxidative and antimicrobial activity. | the aim of our study was to develop an original probiotic cheese based on the estonian open-texture, smear-ripened, semisoft cheese "pikantne." cheese was produced by two methods using cheese starter cultures (probat 505) in combination with 0.04% of probiotic lactobacillus fermentum strain me-3 (10(9) cfu/ml) with high antimicrobial activity and antioxidative properties. the probiotic lactobacillus was added into milk simultaneously with starter cultures (cheese a) and into drained curd (cheese ... | 2004 | 15328213 |
| identification, characterisation and specificity of a cell wall lytic enzyme from lactobacillus fermentum br11. | screening of a genomic library with an antiserum raised against whole lactobacillus fermentum br11 cells identified a clone expressing an immunoreactive 37-kda protein. analysis of the 3010-bp dna insert contained within the clone revealed four open reading frames (orfs). one orf encodes lysa, a 303 amino acid protein which has up to 35% identity with putative endolysins from prophages lj928 and lj965 from lactobacillus johnsonii and lp1 and lp2 from lactobacillus plantarum as well as with the e ... | 2004 | 15336396 |
| genetic diversity (rapd-pcr) of lactobacilli isolated from "almagro" eggplant fermentations from two seasons. | genetic diversity of 323 strains of lactobacilli isolated from an almagro eggplant manufacturing plant was analyzed by using random amplified polymorphic dna (rapd). thirty-four distinct rapd patterns were obtained with 95% of isolates grouped into 18 main clusters. genetic diversity was higher in brines from season ii, lactobacillus plantarum and lactobacillus fermentum/cellobiosus being the species with the greatest number of genotypes. a single l. fermentum/cellobiosus genotype comprised isol ... | 2004 | 15336417 |
| reduction of alpha-galactooligosaccharides in soyamilk by lactobacillus fermentum crl 722: in vitro and in vivo evaluation of fermented soyamilk. | consumption of soya-derived products has been hampered by the presence of alpha-galactooligosaccharides (alpha-gos) because mammals lack pancreatic alpha-galactosidase (alpha-gal) which is necessary for their hydrolysis. these sugars thus reach the large intestine causing gastrointestinal disorders in sensitive individuals. the use of lactic acid bacteria (lab) expressing alpha-gal is a promising solution for the degradation of alpha-gos in soyamilk. | 2004 | 15357738 |
| the microflora of fermented nixtamalized corn. | nixtamalization is a traditional process that improves the nutritional quality of corn. to provide a means of utilizing the nutritional benefits of nixtamalized corn and improve product acceptability, lactic acid fermentation was applied. the objective of the study was to study the microbial profile and establish the important lactobacilli of fermenting nixtamalized corn dough. two batches of cleaned whole corn were subjected to the process of nixtamalization, using two concentrations of lime (0 ... | 2004 | 15358510 |
| effect of ampicillin on the kinetics of colonization of streptococcus pneumoniae and lactobacillus fermentum in the respiratory tract of mice. | ampicillin was selected to further study the effect of this antibiotic on the colonization capability of s. pneumoniae and l. fermentum intranasally inoculated in a mice experimental model. the sensitivity of s. pneumoniae and l. fermentum to antibiotics was evaluated by different "in vitro" techniques. the results showed that both microorganisms have a typical pattern of sensitivity to antibiotics in these assays. the "in vivo" experiments showed that the treatment with ampicillin increased the ... | 2004 | 15509298 |
| glucan synthesis in the genus lactobacillus: isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains. | members of the genera streptococcus and leuconostoc synthesize various alpha-glucans (dextran, alternan and mutan). in lactobacillus, until now, the only glucosyltransferase (gtf) enzyme that has been characterized is gtfa of lactobacillus reuteri 121, the first gtf enzyme synthesizing a glucan (reuteran) that contains mainly alpha-(1-->4) linkages together with alpha-(1-->6) and alpha-(1-->4,6) linkages. recently, partial sequences of glucansucrase genes were detected in other members of the ge ... | 2004 | 15528655 |
| probiotic potential of 3 lactobacilli strains isolated from breast milk. | breast milk is an important factor in the initiation, development, and composition of the neonatal gut microbiota. in a previous study, the authors isolated lactic acid bacteria from milk of healthy mothers. since some of the identified isolates belonged to the genus lactobacillus, the objective of this work was to evaluate the probiotic potential of 2 lactobacillus gasseri and 1 lactobacillus fermentum strains. different assays, including survival to conditions simulating those existing in the ... | 2005 | 15681631 |
| enhancement in ex vivo phagocytic capacity of peritoneal leukocytes in mice by oral delivery of various lactic-acid-producing bacteria. | lactic-acid-producing bacteria (labs) are known to have immunomodulating activity. in the current study, various labs were tested for their immunity-enhancing activity, especially the phagocytic activity of leukocytes. viable but not heat-killed cells of weissella kimchii strain pl9001, lactobacillus fermentum strain pl9005, and l. plantarum strain pl9011 significantly increased the ex vivo phagocytic capacity of mouse peritoneal leukocytes to ingest fluorescein isothiocyanate (fitc)-labeled esc ... | 2004 | 15696260 |
| bspa (cyuc) in lactobacillus fermentum br11 is a highly expressed high-affinity l-cystine-binding protein. | the bspa protein of lactobacillus fermentum br11 (br11) is a cell envelope constituent that is similar to known solute-binding proteins and putative adhesins. bspa is required for l-cystine uptake and oxidative defense and is likely to be an l-cystine-binding protein. the aim of this study was to directly measure l-cystine-bspa binding and bspa expression. de-energized br11 cells bound radiolabelled l-cystine with a kd of 0.2 microm. a bspa mutant could not bind l-cystine. l-cystine-br11 binding ... | 2005 | 15702255 |
| a novel species-specific pcr assay for identifying lactobacillus fermentum. | lactobacillus fermentum is a gram-positive bacterium that is associated with active caries lesions. methods for identifying lactobacillus species traditionally have been based upon culture methods coupled with biochemical tests, which are generally unreliable. the aim of this study was to develop a species-specific pcr assay for the direct detection of l. fermentum in oral clinical samples. pcr primers specific for l. fermentum were identified by alignment of bacterial 16s rrna genes and selecti ... | 2005 | 15713615 |
| immunoenhancing effects of a new probiotic strain, lactobacillus fermentum pl9005. | the immunoenhancing effects of lactobacillus fermentum pl9005 were assessed via mouse intragastric inoculation. the number of immunoglobulin a-positive cells in the small intestine, cd4+ t lymphocytes in the peripheral blood, and the lymphocyte proliferation response to mitogen stimulation (lipopolysaccharide) increased in mice fed l. fermentum pl9005. the lactic acid concentration also increased dose dependently in the small intestine of mice fed l. fermentum pl9005. no differences were found i ... | 2005 | 15771184 |
| biodiversity of lactobacillus sanfranciscensis strains isolated from five sourdoughs. | five different sourdoughs were investigated for the composition of lactic acid bacteria (lab) and the biodiversity of lactobacillus sanfranciscensis strains. | 2005 | 15836738 |
| effects of probiotics on atopic dermatitis: a randomised controlled trial. | the aim of the study was to investigate the effects of probiotics on moderate or severe atopic dermatitis (ad) in young children. | 2005 | 15863468 |
| rapid identification of dairy lactic acid bacteria by m13-generated, rapd-pcr fingerprint databases. | about a thousand lactic acid bacteria (lab) isolated from dairy products, especially cheeses, were identified and typed by species-specific pcr and rapd-pcr, respectively. rapd-pcr profiles, which were obtained by using the m13 sequence as a primer, allowed us to implement a large database of different fingerprints, which were analysed by bionumerics software. cluster analysis of the combined rapd-pcr fingerprinting profiles enabled us to implement a library, which is a collection of library uni ... | 2005 | 15893395 |
| strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock. | probiotic therapy modulates the composition of the intestinal flora and inhibits the inflammatory response. these properties may be of benefit in the preservation of gut barrier integrity after injury or stress. in this study, we examined the effect of two lactobacillus strains selected for their pathogen exclusion properties on intestinal barrier integrity following hemorrhagic shock. additionally, the responsiveness of the macrophage cell line raw 264.7 to combined exposure to lactobacillus dn ... | 2005 | 15908398 |
| characterization of lactobacillus strains and monitoring by rapd-pcr in controlled fermentations of "almagro" eggplants. | the characterization of 23 lactobacillus strains was performed. the strains were assayed for biogenic amine-forming capacity, hydrogen peroxide production, pectin esterase, cellulase and polygalacturonase production, growth rate, acidifying capacity and salt tolerance. three strains were selected which belonged to the species, lactobacillus brevis, lactobacillus plantarum and lactobacillus fermentum. different starter cultures prepared as combinations of these three strains were assayed in pilot ... | 2005 | 15979183 |
| growth of lactic acid bacteria and rhizopus oligosporus during barley tempeh fermentation. | the zygomycete rhizopus oligosporus is traditionally used to ferment soybean tempeh, but it is also possible to ferment other legumes and cereals to tempeh. the traditional soybean tempeh harbours a multitude of microorganisms with potentially beneficial or detrimental effects on quality. lactic acid bacteria (lab) have positive effects on the safety of soybean tempeh, but the effects of lab on r. oligosporus growth have not been investigated. we have developed a cereal grain tempeh by fermentin ... | 2005 | 15979185 |
| lactobacillus fermentum crl 722 is able to deliver active alpha-galactosidase activity in the small intestine of rats. | alpha-galactooligosaccharides (alpha-gos) found in legumes such as soybeans can cause gastrointestinal disorders since mammals lack alpha-galactosidase (alpha-gal) in the small intestine which is necessary for their hydrolysis. lactobacillus fermentum crl 722 is a lactic acid bacterium (lab) capable of degrading alpha-gos due to its elevated alpha-gal activity. when conventional rats were fed live l. fermentum crl 722 or cell-free extracts of this strain, a short-lived alpha-gal activity was det ... | 2005 | 15990251 |
| bacterial population in traditional sourdough evaluated by molecular methods. | to study the microbial communities in artisanal sourdoughs, manufactured by traditional procedure in different areas of sicily, and to evaluate the lactic acid bacteria (lab) population by classical and culture-independent approaches. | 2005 | 16033455 |
| lactobacillus fermentum, a probiotic capable to release glutathione, prevents colonic inflammation in the tnbs model of rat colitis. | inflammatory bowel disease is associated with intestinal oxidative stress. in the present study we test the preventative effect of lactobacillus fermentum, a probiotic that produces per se glutathione, in the trinitrobenzenesulphonic acid (tnbs) model of rat colitis. | 2006 | 16052308 |
| evaluation of the functional efficacy of an antioxidative probiotic in healthy volunteers. | in persons without clinical symptom it is difficult to assess an impact of probiotics regarding its effect on health. we evaluated the functional efficacy of the probiotic lactobacillus fermentum me-3 in healthy volunteers by measuring the influence of two different formulations on intestinal lactoflora, fecal recovery of the probiotic strain and oxidative stress markers of blood and urine after 3 weeks consumption. | 2005 | 16080791 |
| development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, lactobacillus thermotolerans, in chicken feces. | a new real-time pcr assay was successfully developed using a taqman fluorescence probe for specific detection and enumeration of a novel bacterium, lactobacillus thermotolerans, in chicken feces. the specific primers and probe were designed based on the l. thermotolerans 16s rrna gene sequences, and these sequences were compared to those of all available 16s rrna genes in the genbank database. the assay, targeting 16s rrna gene, was evaluated using dna from a pure culture of l. thermotolerans, d ... | 2005 | 16085805 |
| diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of gari, a traditional african food. | traditional fermentation of cassava is dominated by a lactic acid bacteria (lab) population. fermentation is important for improving product flavour and aroma as well as safety, especially by reduction of its toxic cyanogenic glucosides. the production of gari from cassava in benin typically occurs on a household or small industrial scale, and consequently suffers from inconsistent product quality and may not always be safe for consumption. therefore, the diversity of lab from a typical cassava ... | 2005 | 16104351 |
| small surface-associated factors mediate adhesion of a food-isolated strain of lactobacillus fermentum to caco-2 cells. | in a search for bacteria having putative probiotic activity, we screened a collection of food-isolated microorganisms for the ability to survive at low ph in the presence of bile salts and for the production of antimicrobial compounds active against a number of animal pathogens. among these, we found a strain that we classified as a member of lactobacillus fermentum sp., and we further investigated its features. this organism was able to adhere to human enterocyte-like (caco-2) cells with high e ... | 2005 | 16125909 |
| lactic acid bacteria and yeasts associated with spontaneous fermentations during the production of sour cassava starch in brazil. | sour cassava starch is a traditional fermented food used in the preparation of fried foods and baked goods such as traditional cheese breads in brazil. thirty samples of sour cassava starch were collected from two factories in the state of minas gerais. the samples were examined for the presence of lactic acid bacteria, yeasts, mesophilic microorganisms, bacillus cereus and faecal coliforms. lactic acid bacteria and yeasts isolates were identified by biochemical tests, and the identities were co ... | 2005 | 16153731 |
| characterisation of the microflora of attiéké, a fermented cassava product, during traditional small-scale preparation. | attiéké is a fermented cassava product consumed mainly in cote d'ivoire. the aim of this study was to characterise the attiéké fermentation by examining products from 15 small-scale production sites at various stages of its preparation. for the preparation of attiéké, fresh cassava is grated to a pulp and inoculated with 10% of a spontaneous traditional inoculum. the inocula contained aerobic mesophiles at mean numbers of 8.2 x 10(7) cfu/g and lactic and acetic acids at mean concentrations of 0. ... | 2006 | 16213052 |
| monitoring the lactic acid bacterial diversity during shochu fermentation by pcr-denaturing gradient gel electrophoresis. | the presence of lactic acid bacteria (lab) during shochu fermentation was monitored by pcr-denaturing gradient gel electrophoresis (dgge) and by bacteriological culturing. no lab were detected from fermented mashes by pcr-dgge using a universal bacterial pcr primer set. however, pcr-dgge using a new primer specific for the 16s rdna of lactococcus, streptococcus, tetragenococcus, enterococcus, and vagococcus and two primers specific for the 16s rdna of lactobacillus, pediococcus, leuconostoc, and ... | 2005 | 16233780 |
| oral lactobacilli in chronic periodontitis and periodontal health: species composition and antimicrobial activity. | lactobacilli are known to play an important role in the maintenance of health by stimulating natural immunity and contributing to the balance of microflora. however, their role in chronic periodontitis is unclear. we aimed to identify oral lactobacilli in chronic periodontitis and periodontally healthy subjects, and to determine their antimicrobial activity against putative oral pathogens. | 2005 | 16238595 |
| resistance to antimicrobial agents in lactobacilli isolated from caper fermentations. | a collection of lactobacilli comprising species of lactobacillus plantarum (43 isolates), lactobacillus brevis (9 isolates) and lactobacillus fermentum (6 isolates) obtained from spontaneous fermentations of capers (the fruits of capparis spinosa) were investigated for resistance to antimicrobial agents. all isolates were resistant to vancomycin and teicoplanin (mic > 16 microg/ml). resistance to ciprofloxacin (mic > 2 microg/ml) was detected in all isolates of l. brevis and l. fermentum as well ... | 2005 | 16284934 |
| microbiological study of lactic acid fermentation of caper berries by molecular and culture-dependent methods. | fermentation of capers (the fruits of capparis sp.) was studied by molecular and culture-independent methods. a lactic acid fermentation occurred following immersion of caper berries in water, resulting in fast acidification and development of the organoleptic properties typical of this fermented food. a collection of 133 isolates obtained at different times of fermentation was reduced to 75 after randomly amplified polymorphic dna (rapd)-pcr analysis. isolates were identified by pcr or 16s rrna ... | 2005 | 16332762 |
| electrode system for the determination of microbial populations. | determinations of microbial populations were carried out by using a new electrode system composed of two electrodes. each electrode was constructed from a platinum anode and a silver peroxide cathode. the anode of the reference electrode was covered with cellulose dialysis membrane. the response time of the electrode system was 15 min in culture broth, and current differences between the two electrodes were proportional to populations of microbial cells in cultures of saccharomyces cerevisiae an ... | 1979 | 16345334 |
| culture of five commonly used acid-producing bacteria on banana pulp. | a significant fraction of the bananas and plantains produced in the humid tropics are wasted due to rapid spoilage. the use of acid-producing, anaerobic fermentation may be a safe and economical way to preserve the fruit in both large- and small-scale applications. the abilities of five commonly used, acid-producing bacteria to ferment media containing ripe or green bananas were examined. three homofermentative bacteria, lactobacillus bulgaricus, streptococcus thermophilus, and streptococcus fae ... | 1980 | 16345572 |