| microbial community dynamics during production of the mexican fermented maize dough pozol. | the dynamics of the microbial community responsible for the traditional fermentation of maize in the production of mexican pozol was investigated by using a polyphasic approach combining (i) microbial enumerations with culture media, (ii) denaturing gradient gel electrophoresis (dgge) fingerprinting of total community dna with bacterial and eukaryotic primers and sequencing of partial 16s ribosomal dna (rdna) genes, (iii) quantification of rrnas from dominant microbial taxa by using phylogenetic ... | 2000 | 10966374 |
| proteolytic activities in togwa, a tanzanian fermented food. | proteolytic activities were investigated in sorghum-based togwa prepared by natural fermentation and using starter cultures previously isolated from the native product, i.e., lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum, pediococcus pentosaceus, and issatchenkia orientalis in coculture with either l. brevis or l. plantarum. both proteinase and aminopeptidase activities were substantially higher in naturally fermented togwa than in those with s ... | 2003 | 12781948 |
| identification of lactic acid bacteria isolated from human vaginal secretions. | a total of 57 lactic acid bacteria were isolated from the vaginal secretions of 259 patients. of these strains, 37 were isolated from patients attending pre-natal clinics and the remaining strains from patients attending post-natal clinics. the strains were identified by using simple physiological and biochemical tests and their phenotypic relatedness determined by numerical analysis of total soluble cell protein patterns. the genotypic relatedness of representative strains selected from each of ... | 2003 | 12785305 |
| reduction of fecal shedding of enterohemorrhagic escherichia coli o157:h7 in lambs by feeding microbial feed supplement. | enterohemorrhagic escherichia coli o157:h7, an emerging food-borne pathogen, has been implicated in several outbreaks in the us. ruminants, including cattle, sheep and deer are reservoirs of e. coli o157:h7 and fecal shedding of the pathogen forms the vehicle of entry into the human food chain. we studied the efficacy of lactobacillus acidophilus, streptococcus faecium, a mixture of l. acidophilus and s. faecium and a mixture of l. acidophilus, s. faecium, lactobacillus casei, lactobacillus ferm ... | 2001 | 11163713 |
| experimental administration of estradiol on the colonization of lactobacillus fermentum and escherichia coli in the urogenital tract of mice. | the effect of estrogen on the microbial colonization of the urogenital tract is widely discussed, mainly in regard to women with a high incidence of urinary tract infections (uti). the aim of this work was to study the effect of estradiol on the microbial colonization of lactobacilli and e. coli in mice. female balb/c mice were intramuscularly (i.m.) treated with beta-estradiol (one or three doses). the next day, l. fermentum was inoculated intraurethrally with three doses of 10(7) cfu (colony f ... | 2001 | 11217078 |
| characterization of lactobacilli from scotch malt whisky distilleries and description of lactobacillus ferintoshensis sp. nov., a new species isolated from malt whisky fermentations. | sixty-four strains of lactobacillus were isolated from fermentation samples from 23 malt whisky distilleries located in the major whisky producing regions of scotland. the strains were assigned to 26 ribotype patterns. strains of some ribotype patterns were widely distributed and recovered from distilleries throughout scotland, while strains representing other ribotypes were particular to a specific region or even a certain distillery. repeated sampling of a single distillery over a 12 month per ... | 2001 | 11283296 |
| an in vitro study of the probiotic potential of a bile-salt-hydrolyzing lactobacillus fermentum strain, and determination of its cholesterol-lowering properties. | this study evaluated the use of a bile-salt-hydrolyzing lactobacillus fermentum strain as a probiotic with potential hypocholesterolemic properties. the effect of l. fermentum on representative microbial populations and overall metabolic activity of the human intestinal microbiota was investigated using a three-stage continuous culture system. also, the use of galactooligosaccharides as a prebiotic to enhance growth and/or activity of the lactobacillus strain was evaluated. administration of l. ... | 2003 | 12902267 |
| antioxidative probiotic fermented goats' milk decreases oxidative stress-mediated atherogenicity in human subjects. | the increasing interest in a healthy diet is stimulating innovative development of novel scientific products in the food industry. the viable lactic acid bacteria in fermented milk products, such as yoghurt, have been associated with increased lactose tolerance, a well-balanced intestinal microflora, antimicrobial activity, stimulation of the immune system and antitumoural, anticholesterolaemic and antioxidative properties in human subjects. recently, we have studied a human lactobacillus spp. s ... | 2003 | 12908907 |
| thiamine sparing action of ascorbic acid on lactobacillus fermenti 36. | | 1953 | 13055949 |
| nutritional studies with lactobacillus fermenti. | | 1953 | 13069440 |
| species differentiation of oral lactobacilli from man including description of lactobacillus salivarius nov spec and lactobacillus cellobiosus nov spec. | | 1953 | 13069442 |
| [microbiological determination of vitamin b1 with the help of lactobacillus fermenti]. | | 1956 | 13373834 |
| improved medium for assay of thiamine with lactobacillus fermenti. | | 1957 | 13459314 |
| effect of oleic acid on the response of lactobacillus fermenti to thiamin and its moieties. | | 1958 | 13538925 |
| glutamic acid racemase from lactobacillus fermenti. purification and properties. | | 1961 | 13775143 |
| comparative study of nine lactobacillus fermentum bacteriophages. | to investigate the basic properties of six temperate and three virulent phages, active on lactobacillus fermentum, on the basis of morphology, host ranges, protein composition and genome characterization. | 2001 | 11556903 |
| biofilm formation and contamination of cheese by nonstarter lactic acid bacteria in the dairy environment. | defects in cheese, such as undesirable flavors, gas formation, or white surface haze from calcium lactate crystals, can result from growth of nonstarter lactic acid bacteria (nslab). the potential for biofilm formation by nslab during cheese manufacturing, the effect of cleaning and sanitizing on the biofilm, and bacterial growth and formation of defects during ripening of the contaminated cheese were studied. stirred-curd cheddar cheese was made in the presence of stainless steel chips containi ... | 2001 | 11573770 |
| protective effect of intranasally inoculated lactobacillus fermentum against streptococcus pneumoniae challenge on the mouse respiratory tract. | lactic acid bacteria are increasingly used to restore the ecological equilibrium of different mucosal areas in humans and/or animals. likewise, they can be used to potentially protect against pathogenic microorganisms. in the present paper, the preventive effect of intranasally inoculated lactobacillus fermentum against challenge with streptococcus pneumoniae was studied, using a mouse experimental model. l. fermentum inoculated four times at a dose of 10(7) colony forming units per mouse was ab ... | 2001 | 11720814 |
| thiamine activity of thiamine propyl disulfide on the growth of lactobacillus fermenti 36. | | 1964 | 14217467 |
| influence of sugars on the growth of lactobacillus fermenti 36. | | 1965 | 14341424 |
| stimulatory effect of calcium on growth of lactobacillus fermenti. | | 1955 | 14357401 |
| plate assay of thiamine. ii. using lactobacillus fermenti. | | 1959 | 14403841 |
| two antioxidative lactobacilli strains as promising probiotics. | two antioxidative strains tentatively identified as lactobacillus fermentum, e-3 and e-18, were isolated from intestinal microflora of a healthy child. survival time of these strains in the presence of reactive oxygen species (ros), like hydrogen peroxide, superoxide anions and hydroxyl radicals, was significantly increased compared with a non-antioxidative strain, and also was quite similar to a highly ros resistant strain of salmonella typhimurium. e-3 and e-8 contain a remarkable level of glu ... | 2002 | 11845820 |
| diversity, dynamics, and activity of bacterial communities during production of an artisanal sicilian cheese as evaluated by 16s rrna analysis. | the diversity and dynamics of the microbial communities during the manufacturing of ragusano cheese, an artisanal cheese produced in sicily (italy), were investigated by a combination of classical and culture-independent approaches. the latter included pcr, reverse transcriptase-pcr (rt-pcr), and denaturing gradient gel electrophoresis (dgge) of 16s rrna genes (rdna). bacterial and lactobacillus group-specific primers were used to amplify the v6 to v8 and v1 to v3 regions of the 16s rrna gene, r ... | 2002 | 11916708 |
| cystine uptake prevents production of hydrogen peroxide by lactobacillus fermentum br11. | bspa is an abundant surface protein from lactobacillus fermentum br11, and is required for normal cystine uptake. in previous studies, a mutant strain deficient in bspa (l. fermentum png201) was found to be sensitive to oxidative stress. in this study, the biochemical basis for this was explored. it was found that under aerobic batch culture conditions in de mann-rogosa-sharpe medium, both l. fermentum br11 and png201 entered stationary phase due to hydrogen peroxide accumulation. however, this ... | 2003 | 14568153 |
| adhesion of probiotic lactobacilli to chick intestinal mucus. | in the present work, interactions between three lactobacillus strains (lactobacillus fermentum crl1015, lactobacillus animalis crl1014, and lactobacillus fermentum crl1016) and chicken small intestinal mucus were determined. three lactobacilli isolated from chicken and selected by their potentially probiotic properties were able to grow in mucus preparations. three peaks from gel filtration chromatography of intestinal mucus were obtained. the adhesion to three mucus fractions (i, ii, and iii), ... | 2003 | 14569288 |
| antimicrobial interactions of microbial species involved in the fermentation of cassava dough into agbelima with particular reference to the inhibitory effect of lactic acid bacteria on enteric pathogens. | lactic acid bacteria, bacillus species and yeasts are involved in the fermentation of cassava dough into agbelima. microbial interactions within and between these groups of microorganisms were investigated in addition to the survival of five enteric pathogens inoculated into agbelima under various conditions. nine out of 10 cultures of lactic acid bacteria isolated at the end of agbelima fermentation showed inhibitory effect against 10 cultures of lactic acid bacteria isolated at the start of fe ... | 2003 | 14580972 |
| purification and characterization of a surface protein from lactobacillus fermentum 104r that binds to porcine small intestinal mucus and gastric mucin. | an adhesion-promoting protein involved in the binding of lactobacillus fermentum strain 104r to small intestinal mucus from piglets and to partially purified gastric mucin was isolated and characterized. spent culture supernatant fluid and bacterial cell wall extracts were fractionated by ammonium sulfate precipitation and gel filtration. the active fraction was purified by affinity chromatography. the adhesion-promoting protein was detected in the fractions by adhesion inhibition and dot blot a ... | 2002 | 11976105 |
| characterization of a cryptic plasmid from lactobacillus fermentum kc5b and its use for constructing a stable lactobacillus cloning vector. | lactobacillus fermentum kc5b, a strain originally isolated from the human vagina, contains a cryptic plasmid pkc5b. the sequence and genetic organization of the 4392-bp plasmid were determined. it contains two convergently oriented replicons, which are homologous to each other and to the stable replicon of the enterococcus faecium plasmid pmbb1. the two replicons of pkc5b were used either individually or together to construct lactobacillus-escherichia coli shuttle plasmids. only the plasmid psp1 ... | 2002 | 12151233 |
| effects of asparaginase and polyphenol oxidase on adhesive characteristics of microorganisms. | we studied the effects of polyphenol oxidase and asparaginase on microorganism adhesion to buccal epithelial cells. these enzymes reduced adhesion of pathogenic microorganisms (uropathogenic and escherichia coli, salmonella enteritidis, entamoeba spp., influenza virus, candida albicans, streptococcus spp.) and had virtually no effect on adhesive characteristics of probiotic variants of escherichia coli and lactobacillus fermentum. | 2002 | 12170308 |
| modulating immune responses with probiotic bacteria. | for many years, probiotic bacteria have been known to confer health benefits to the consumer. one possible mechanism for this may be the ability of probiotic bacteria to modulate immune responses. oral administration of lactobacillus casei strain shirota (lcs) has been found to enhance innate immunity by stimulating the activity of splenic nk cells. oral feeding with killed lcs was able to stimulate the production of th1 cytokines, resulting in repressed production of ige antibodies against oval ... | 2000 | 10651931 |
| fermentation of vigna sinensis var. carilla flours by natural microflora and lactobacillus species. | natural fermentation and an inoculum containing 10% (vol/vol) lactobacillus fermentum or lactobacillus plantarum were used to obtain fermented flours from vigna sinensis l. var. carilla seeds that had been washed with distilled water and dried at 55 degrees c for 24 h. to optimize the fermentation parameters (lactic acid bacterium level, bean flour concentration, and fermentation time), several small-scale fermentation processes were carried out. on the basis of the results obtained, fermentor-s ... | 2003 | 14672230 |
| lactobacillus flora in the cloaca and vagina of hens and its inhibitory activity against salmonella enteritidis in vitro. | lactobacilli in the cloaca and vagina of 40 normal laying hens were investigated quantitatively and qualitatively, and their ability to inhibit growth of salmonella enteritidis (se) was examined using a spot-the-lawn technique. all samples of cloacal contents and half the samples of vaginal mucus were positive for lactobacilli. the means +/- sd of total lactobacillus counts in the cloaca and those in the vagina were log10 5.5 +/- 1.1 and 2.5 +/- 2.6 cfu/g, respectively. in the cloaca, lactobacil ... | 2000 | 10685882 |
| lactobacillus reuteri dsm 20016: purification and characterization of a cystathionine gamma-lyase and use as adjunct starter in cheesemaking. | a homo-tetrameric approximately 160-kda cystathionine gamma-lyase was purified to homogeneity from lactobacillus reuteri dsm 20016 by four chromatographic steps. the activity was pyridoxal-5'-phosphate dependent and the enzyme catalyzed the alpha,gamma-elimination reaction of l-cystathionine, producing l-cysteine, ammonia and alpha-ketobutyrate. the enzyme was active towards a range of amino acids and amino acid derivatives, including methionine. the ph and temperature optima were found to be 8. ... | 2002 | 12222803 |
| microbiological assay method for thiamine using lactobacillus fermenti 36 as test organism. | | 1951 | 14911916 |
| characterisation of the microbiota of rice sourdoughs and description of lactobacillus spicheri sp. nov. | the microbiota of two industrially processed rice sourdoughs was characterised by bacteriological culture in combination with pcr-denaturing gradient gel electrophoresis (dgge) and 16s/28s rdna sequence analysis. rice sourdough i was continuously propagated for several years by back-slopping every week, whereas sourdough ii was processed by using a commercial starter culture and back-slopping daily for three days. in rice sourdough ii candida krusei and saccharomyces cerevisiae as well as lactob ... | 2004 | 15046303 |
| some probiotic properties of chicken lactobacilli. | the beneficial effect of lactobacilli has been attributed to their ability to colonize human and animal gastrointestinal tracts. in this work, adhesion assays with three lactobacillus strains and intestinal fragments obtained from chickens were assessed. lactobacillus animalis and l. fermentum were able to adhere to three kinds of epithelial cells (crop, small and large intestines) with predominance to small intestine. among the strains considered, l. fermentum subsp. cellobiosus showed the lowe ... | 1999 | 10696476 |
| indigenous raw milk microbiota influences the bacterial development in traditional cheese from an alpine natural park. | nostrano di primiero is a 6-month ripened cheese produced from raw milk collected in the paneveggio-pale di san martino natural park area in the italian dolomites. in summer, this cheese is made using milk collected from two different areas, passo rolle and vanoi, in the paneveggio natural park. during the experiment, the milk from the two areas was separately processed, and cheeses were made in the same cheese factory using the same technological process. the microbiota of raw milk and cheeses ... | 2004 | 15109791 |
| reclassification of lactobacillus cellobiosus rogosa et al. 1953 as a later synonym of lactobacillus fermentum beijerinck 1901. | the name lactobacillus cellobiosus is validly published, but the species is often neglected in taxonomic studies, due to its high similarity to lactobacillus fermentum. in the present paper, literature data concerning the two species were reviewed. phylogenetic placement of l. cellobiosus was obtained based on 16s rdna sequences, and genetic similarity was further investigated by comparing partial reca gene sequences for the type strains of l. cellobiosus and l. fermentum. based on the high iden ... | 2004 | 15143028 |
| identification and characterization of the novel lysm domain-containing surface protein sep from lactobacillus fermentum br11 and its use as a peptide fusion partner in lactobacillus and lactococcus. | examination of supernatant fractions from broth cultures of lactobacillus fermentum br11 revealed the presence of a number of proteins, including a 27-kda protein termed sep. the amino-terminal sequence of sep was determined, and the gene encoding it was cloned and sequenced. sep is a 205-amino-acid protein and contains a 30-amino-acid secretion signal and has overall homology (between 39 and 92% identity) with similarly sized proteins of lactobacillus reuteri, enterococcus faecium, streptococcu ... | 2004 | 15184172 |
| mononuclear cell response to enterobacteria and gram-positive cell walls of normal intestinal microbiota in early rheumatoid arthritis and other inflammatory arthritides. | to study whether enterobacteria and gram-positive bacterial cell walls (bcw) derivedfrom normal intestinal microbiota are involved in the etiopathogenesis of early rheumatoid arthritis (ra). | 2002 | 12051398 |
| isolation, identification and characterisation of the dominant microorganisms of kule naoto: the maasai traditional fermented milk in kenya. | from 22 samples of kule naoto, the traditional fermented milk products of the maasai in kenya, 300 lactic acid bacterial strains were isolated and phenotypically characterised by their ability to ferment different carbohydrates and by additional biochemical tests. lactic acid bacteria (lab), especially the genus lactobacillus, followed by enterococcus, lactococcus and leuconostoc, dominated the microflora of these samples. the major lactobacillus species was lactobacillus plantarum (60%), with a ... | 2004 | 15246238 |
| nucleic acid-based diagnosis of bacterial vaginosis and improved management using probiotic lactobacilli. | bacterial vaginosis (bv) is a common condition in women that represents an imbalance of the vaginal microflora, lactobacilli depletion, and excess growth of mainly anaerobic gram-negative pathogens. diagnosis is made using a series of tests or a gram stain of a vaginal smear. treatment with antibiotics is quite effective, but recurrences are common. a study of 55 vaginal samples from 11 postmenopausal women showed the presence of bv by the gram stain-based nugent scoring system, and polymerase c ... | 2004 | 15298771 |
| fermentation by lactobacillus fermentum ogi e1 of different combinations of carbohydrates occurring naturally in cereals: consequences on growth energetics and alpha-amylase production. | glucose, fructose, sucrose and starch are naturally present in cereals. fermentation of different combinations of these carbohydrates by lactobacillus fermentum ogi e1, a sourdough heterofermentative lactobacillus, was investigated to determine effects on fermentation kinetics, growth energetics and alpha-amylase production. irrespective of the substrate combination, the strain was able to simultaneously produce alpha-amylase and consume starch, glucose, fructose and sucrose. in mixtures of star ... | 2003 | 12381402 |
| identification, characterisation and specificity of a cell wall lytic enzyme from lactobacillus fermentum br11. | screening of a genomic library with an antiserum raised against whole lactobacillus fermentum br11 cells identified a clone expressing an immunoreactive 37-kda protein. analysis of the 3010-bp dna insert contained within the clone revealed four open reading frames (orfs). one orf encodes lysa, a 303 amino acid protein which has up to 35% identity with putative endolysins from prophages lj928 and lj965 from lactobacillus johnsonii and lp1 and lp2 from lactobacillus plantarum as well as with the e ... | 2004 | 15336396 |
| genetic diversity (rapd-pcr) of lactobacilli isolated from "almagro" eggplant fermentations from two seasons. | genetic diversity of 323 strains of lactobacilli isolated from an almagro eggplant manufacturing plant was analyzed by using random amplified polymorphic dna (rapd). thirty-four distinct rapd patterns were obtained with 95% of isolates grouped into 18 main clusters. genetic diversity was higher in brines from season ii, lactobacillus plantarum and lactobacillus fermentum/cellobiosus being the species with the greatest number of genotypes. a single l. fermentum/cellobiosus genotype comprised isol ... | 2004 | 15336417 |
| study of starch fermentation at low ph by lactobacillus fermentum ogi e1 reveals uncoupling between growth and alpha-amylase production at ph 4.0. | lactobacillus fermentum ogi e1 is an amylolytic heterofermentative lactic acid bacterium previously isolated from ogi, a benin maize sourdough. in the present study, the effect of different ph between 3.5 and 6.0 on starch fermentation products and alpha-amylase production was investigated. whereas a ph of 5.0 was optimum for specific growth rate and lactic acid production, growth was only slightly affected at suboptimal ph of 4.0 and 6.0. over a ph range of 6.0 to 3.5, yields of product formati ... | 2003 | 12430774 |
| the microflora of fermented nixtamalized corn. | nixtamalization is a traditional process that improves the nutritional quality of corn. to provide a means of utilizing the nutritional benefits of nixtamalized corn and improve product acceptability, lactic acid fermentation was applied. the objective of the study was to study the microbial profile and establish the important lactobacilli of fermenting nixtamalized corn dough. two batches of cleaned whole corn were subjected to the process of nixtamalization, using two concentrations of lime (0 ... | 2004 | 15358510 |
| volatile compounds produced by lactobacillus fermentum, saccharomyces cerevisiae and candida krusei in single starter culture fermentations of ghanaian maize dough. | to identify and compare the volatile compounds associated with maize dough samples prepared by spontaneous fermentation and by the use of added starter cultures in ghana. | 2003 | 12588555 |
| lactobacillus ingluviei sp. nov., isolated from the intestinal tract of pigeons. | lactic acid bacteria were isolated from the crop and intestines of pigeons. one group of strains, showing similar genomic patterns after screening with trna intergenic spacer pcr, could not be identified to the species level. sequencing of the 16s rrna gene of one representative strain revealed about 96% similarity to sequences from lactobacillus fermentum and lactobacillus mucosae. determination of the dna base composition, dna-dna hybridization experiments, sds-page of whole-cell proteins and ... | 2003 | 12656164 |
| lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces. | five strains of thermotolerant lactic acid bacteria (g 12, g 22, g 35t, g 43 and g 44) isolated from chicken faeces were characterized taxonomically. the strains were facultatively anaerobic, gram-positive, catalase-negative, non-motile, non-spore-forming rods. they were heterofermentative lactobacilli that produced dl-lactic acid. growth of the strains occurred at 45 degrees c but not at 15 degrees c. the optimum temperature for growth was 42 degrees c, as determined from the specific growth ra ... | 2003 | 12656183 |
| metabolic engineering of lactobacillus fermentum for production of mannitol and pure l-lactic acid or pyruvate. | for production of mannitol in combination with pure l-lactic acid or pyruvate, the d- and l-lactate dehydrogenase genes (ldhd and ldhl) of a mannitol-producing lactobacillus fermentum strain were cloned and stepwise inactivated. for inactivation of both ldh genes by a gene replacement technique, deletion constructs removing a 0.4-kb fragment from the promoter and the 5' end region of the ldh genes were used. the first inactivation mutant, designated l. fermentum grl1030, carried the deletion in ... | 2003 | 12673764 |
| effect of lactobacillus buchneri, lactobacillus fermentum, leuconostoc mesenteroides inoculants, or a chemical additive on the fermentation, aerobic stability, and nutritive value of crimped wheat grains. | the preservation of crimped wheat grains by three bacterial inoculants or a chemical additive was compared. crimped wheat grain [56.8 g dry matter (dm)/kg] was conserved in 1.75-kg plastic bag, mini-silos without treatment, with 4l/tonne of crimpstore (cs; an additive containing a mixture of ammonium formate, propionate, ethyl benzoate, and benzoate, sas kelvin cave, ltd., uk) or 1 x 10(5) cfu/g of each of three inoculant additives containing lactobacillus fermentum (a), leuconostoc mesenteroide ... | 2003 | 12778589 |
| structural and ultrastructural studies of the urinary tract of mice inoculated with lactobacillus fermentum. | to assess, using structural and ultrastructural studies of the urinary tract, the effects of the intraurethral inoculation of lactobacilli (probiotic treatment) as lactobacilli are the predominant micro-organisms of the urogenital tract of humans, monkeys and mice. | 2003 | 12780852 |
| generation of food-grade lactococcal starters which produce the lantibiotics lacticin 3147 and lacticin 481. | transconjugant lactococcal starters which produce both lantibiotics lacticin 3147 and lacticin 481 were generated via conjugation of large bacteriocin-encoding plasmids. a representative of one of the resultant strains proved more effective at killing lactobacillus fermentum and inhibiting the growth of listeria monocytogenes lo28h than either of the single bacteriocin-producing parental strains, demonstrating the potential of these transconjugants as protection cultures for food safety applicat ... | 2003 | 12788782 |
| biodiversity of lactobacillus sanfranciscensis strains isolated from five sourdoughs. | five different sourdoughs were investigated for the composition of lactic acid bacteria (lab) and the biodiversity of lactobacillus sanfranciscensis strains. | 2005 | 15836738 |
| decrease in ovalbumin specific ige of mice serum after oral uptake of lactic acid bacteria. | different kinds of lactobacilli and bifidobacteria fermented milk were fed to ovalbumin-specific ige-elevated mice for 3 days, and after the final administration, changes in the ovalbumin-specific ige values for each sample were compared to the value for non-fermented milk. seven of the lactobacillus-fermented milks caused a significant decrease in the serum ovalbumin-specific ige levels. above all, lactobacillus acidophilus l92, lactobacillus acidophilus cp1613, and lactobacillus fermentum cp34 ... | 2003 | 12834270 |
| rapid identification of dairy lactic acid bacteria by m13-generated, rapd-pcr fingerprint databases. | about a thousand lactic acid bacteria (lab) isolated from dairy products, especially cheeses, were identified and typed by species-specific pcr and rapd-pcr, respectively. rapd-pcr profiles, which were obtained by using the m13 sequence as a primer, allowed us to implement a large database of different fingerprints, which were analysed by bionumerics software. cluster analysis of the combined rapd-pcr fingerprinting profiles enabled us to implement a library, which is a collection of library uni ... | 2005 | 15893395 |
| strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock. | probiotic therapy modulates the composition of the intestinal flora and inhibits the inflammatory response. these properties may be of benefit in the preservation of gut barrier integrity after injury or stress. in this study, we examined the effect of two lactobacillus strains selected for their pathogen exclusion properties on intestinal barrier integrity following hemorrhagic shock. additionally, the responsiveness of the macrophage cell line raw 264.7 to combined exposure to lactobacillus dn ... | 2005 | 15908398 |
| characterization of lactobacillus strains and monitoring by rapd-pcr in controlled fermentations of "almagro" eggplants. | the characterization of 23 lactobacillus strains was performed. the strains were assayed for biogenic amine-forming capacity, hydrogen peroxide production, pectin esterase, cellulase and polygalacturonase production, growth rate, acidifying capacity and salt tolerance. three strains were selected which belonged to the species, lactobacillus brevis, lactobacillus plantarum and lactobacillus fermentum. different starter cultures prepared as combinations of these three strains were assayed in pilot ... | 2005 | 15979183 |
| growth of lactic acid bacteria and rhizopus oligosporus during barley tempeh fermentation. | the zygomycete rhizopus oligosporus is traditionally used to ferment soybean tempeh, but it is also possible to ferment other legumes and cereals to tempeh. the traditional soybean tempeh harbours a multitude of microorganisms with potentially beneficial or detrimental effects on quality. lactic acid bacteria (lab) have positive effects on the safety of soybean tempeh, but the effects of lab on r. oligosporus growth have not been investigated. we have developed a cereal grain tempeh by fermentin ... | 2005 | 15979185 |
| lactobacillus fermentum crl 722 is able to deliver active alpha-galactosidase activity in the small intestine of rats. | alpha-galactooligosaccharides (alpha-gos) found in legumes such as soybeans can cause gastrointestinal disorders since mammals lack alpha-galactosidase (alpha-gal) in the small intestine which is necessary for their hydrolysis. lactobacillus fermentum crl 722 is a lactic acid bacterium (lab) capable of degrading alpha-gos due to its elevated alpha-gal activity. when conventional rats were fed live l. fermentum crl 722 or cell-free extracts of this strain, a short-lived alpha-gal activity was det ... | 2005 | 15990251 |
| bacterial population in traditional sourdough evaluated by molecular methods. | to study the microbial communities in artisanal sourdoughs, manufactured by traditional procedure in different areas of sicily, and to evaluate the lactic acid bacteria (lab) population by classical and culture-independent approaches. | 2005 | 16033455 |
| the microbial ecology of cocoa bean fermentations in indonesia. | cocoa beans are the principal raw material of chocolate manufacture. the beans are subject to a microbial fermentation as the first stage in chocolate production. the microbial ecology of bean fermentation (forastero and trinitario cultivars) was investigated at three commercial fermentaries in east java, indonesia by determining the populations of individual species at 12-h intervals throughout the process. the first 2-3 days of fermentation were characterised by the successional growth of vari ... | 2003 | 12892924 |
| evaluation of the functional efficacy of an antioxidative probiotic in healthy volunteers. | in persons without clinical symptom it is difficult to assess an impact of probiotics regarding its effect on health. we evaluated the functional efficacy of the probiotic lactobacillus fermentum me-3 in healthy volunteers by measuring the influence of two different formulations on intestinal lactoflora, fecal recovery of the probiotic strain and oxidative stress markers of blood and urine after 3 weeks consumption. | 2005 | 16080791 |
| development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, lactobacillus thermotolerans, in chicken feces. | a new real-time pcr assay was successfully developed using a taqman fluorescence probe for specific detection and enumeration of a novel bacterium, lactobacillus thermotolerans, in chicken feces. the specific primers and probe were designed based on the l. thermotolerans 16s rrna gene sequences, and these sequences were compared to those of all available 16s rrna genes in the genbank database. the assay, targeting 16s rrna gene, was evaluated using dna from a pure culture of l. thermotolerans, d ... | 2005 | 16085805 |
| diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of gari, a traditional african food. | traditional fermentation of cassava is dominated by a lactic acid bacteria (lab) population. fermentation is important for improving product flavour and aroma as well as safety, especially by reduction of its toxic cyanogenic glucosides. the production of gari from cassava in benin typically occurs on a household or small industrial scale, and consequently suffers from inconsistent product quality and may not always be safe for consumption. therefore, the diversity of lab from a typical cassava ... | 2005 | 16104351 |
| activation of the disulfide forms of thiamine and its phosphates as growth factors for lactobacillus fermenti. | | 1960 | 13796439 |
| lactic acid bacteria and yeasts associated with spontaneous fermentations during the production of sour cassava starch in brazil. | sour cassava starch is a traditional fermented food used in the preparation of fried foods and baked goods such as traditional cheese breads in brazil. thirty samples of sour cassava starch were collected from two factories in the state of minas gerais. the samples were examined for the presence of lactic acid bacteria, yeasts, mesophilic microorganisms, bacillus cereus and faecal coliforms. lactic acid bacteria and yeasts isolates were identified by biochemical tests, and the identities were co ... | 2005 | 16153731 |
| [on the growth effects of thiamine disulfide on lactobacillus fermenti 36 in the agar plate test]. | | 1959 | 13796441 |
| monitoring the lactic acid bacterial diversity during shochu fermentation by pcr-denaturing gradient gel electrophoresis. | the presence of lactic acid bacteria (lab) during shochu fermentation was monitored by pcr-denaturing gradient gel electrophoresis (dgge) and by bacteriological culturing. no lab were detected from fermented mashes by pcr-dgge using a universal bacterial pcr primer set. however, pcr-dgge using a new primer specific for the 16s rdna of lactococcus, streptococcus, tetragenococcus, enterococcus, and vagococcus and two primers specific for the 16s rdna of lactobacillus, pediococcus, leuconostoc, and ... | 2005 | 16233780 |
| oral lactobacilli in chronic periodontitis and periodontal health: species composition and antimicrobial activity. | lactobacilli are known to play an important role in the maintenance of health by stimulating natural immunity and contributing to the balance of microflora. however, their role in chronic periodontitis is unclear. we aimed to identify oral lactobacilli in chronic periodontitis and periodontally healthy subjects, and to determine their antimicrobial activity against putative oral pathogens. | 2005 | 16238595 |
| resistance to antimicrobial agents in lactobacilli isolated from caper fermentations. | a collection of lactobacilli comprising species of lactobacillus plantarum (43 isolates), lactobacillus brevis (9 isolates) and lactobacillus fermentum (6 isolates) obtained from spontaneous fermentations of capers (the fruits of capparis spinosa) were investigated for resistance to antimicrobial agents. all isolates were resistant to vancomycin and teicoplanin (mic > 16 microg/ml). resistance to ciprofloxacin (mic > 2 microg/ml) was detected in all isolates of l. brevis and l. fermentum as well ... | 2005 | 16284934 |
| microbiological study of lactic acid fermentation of caper berries by molecular and culture-dependent methods. | fermentation of capers (the fruits of capparis sp.) was studied by molecular and culture-independent methods. a lactic acid fermentation occurred following immersion of caper berries in water, resulting in fast acidification and development of the organoleptic properties typical of this fermented food. a collection of 133 isolates obtained at different times of fermentation was reduced to 75 after randomly amplified polymorphic dna (rapd)-pcr analysis. isolates were identified by pcr or 16s rrna ... | 2005 | 16332762 |
| the effect of s-benzoyl derivatives of thiamine on the growth of lactobacillus fermenti 36. | | 1962 | 14031536 |
| culture of five commonly used acid-producing bacteria on banana pulp. | a significant fraction of the bananas and plantains produced in the humid tropics are wasted due to rapid spoilage. the use of acid-producing, anaerobic fermentation may be a safe and economical way to preserve the fruit in both large- and small-scale applications. the abilities of five commonly used, acid-producing bacteria to ferment media containing ripe or green bananas were examined. three homofermentative bacteria, lactobacillus bulgaricus, streptococcus thermophilus, and streptococcus fae ... | 1980 | 16345572 |
| clinical effects of probiotics are associated with increased interferon-gamma responses in very young children with atopic dermatitis. | we recently demonstrated that administration of probiotics resulted in significant clinical improvement in very young children with moderate-to-severe atopic dermatitis (ad). the purpose of this study was to determine the underlying immunological effects that are associated with these apparent clinical benefits. | 2005 | 16393321 |
| lactobacillus bacteremia, species identification, and antimicrobial susceptibility of 85 blood isolates. | data regarding antimicrobial susceptibility of clinical lactobacillus isolates are scarce, and appropriate interpretation criteria for susceptibility tests are not available. | 2006 | 16447101 |
| functional and structural characterization of thermostable d-amino acid aminotransferases from geobacillus spp. | d-amino acid aminotransferases (d-aats) from geobacillus toebii sk1 and geobacillus sp. strain kls1 were cloned and characterized from a genetic, catalytic, and structural aspect. although the enzymes were highly thermostable, their catalytic capability was approximately one-third of that of highly active bacilli enzymes, with respective turnover rates of 47 and 55 s(-1) at 50 degrees c. the geobacillus enzymes were unique and shared limited sequence identities of below 45% with d-aats from meso ... | 2006 | 16461714 |
| use of polymerase chain reaction techniques and sodium dodecyl sulfate-polyacrylamide gel electrophoresis for differentiation of oral lactobacillus species. | the genus lactobacillus has been associated with dental caries in humans, although it is seldom speciated due to lack of simple and nonlaborious identification methods. a considerable heterogeneity among lactobacillus species has been demonstrated. the purpose of this study was to develop simple methods combining restriction fragment length polymorphism analysis of polymerase chain reaction (pcr)-amplified 16s rrna (16s rrna gene pcr-rflp) and sodium dodecyl sulfate-polyacrylamide gel electropho ... | 2006 | 16476016 |
| antimicrobial activity of lysostaphin and a listeria monocytogenes bacteriophage endolysin produced and secreted by lactic acid bacteria. | the expression and secretion signals of the sep protein from lactobacillus fermentum br11 were used to direct export of two peptidoglycan hydrolases by lb. fermentum br11, lactobacillus rhamnosus gg, lactobacillus plantarum atcc 14917 and lactococcus lactis mg1363. the production levels, hydrolytic and bacteriocidal activities of the listeria monocytogenes bacteriophage n-acetylmuramoyl-l-alanine amidase endolysin ply511 and the glycylglycine endopeptidase lysostaphin were examined. buffering of ... | 2007 | 16490333 |
| sensitization of staphylococcus aureus and escherichia coli to antibiotics by the sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone. | the sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone were investigated for their abilities to enhance bacterial permeability and susceptibility to exogenous antimicrobial compounds. initially, it was observed by flow cytometry that these sesquiterpenoids promoted the intracellular accumulation of the membrane-impermeant nucleic acid stain ethidium bromide by live cells of lactobacillus fermentum, suggesting that enhanced permeability resulted from disruption of the cytoplasmic membr ... | 2003 | 14506058 |
| the mechanism of action of alpha-amylase from lactobacillus fermentum on maltooligosaccharides. | the action pattern of lactobacillus fermentum alpha-amylase (fermenta) was examined using a series of maltooligosaccharides (g2-g7) as substrates. structurally, this enzyme has a molecular mass (106 kda) almost twofold higher than alpha-amylases from mammalians and cereals. the product pattern was investigated through an analysis of products and substrates using hpaec with pulsed amperometric detection. fermenta was consistent with an endo-type of amylase. the bond cleavage frequencies were stud ... | 2006 | 16531129 |
| peptide surface display and secretion using two lpxtg-containing surface proteins from lactobacillus fermentum br11. | a locus encoding two repetitive proteins that have lpxtg cell wall anchoring signals from lactobacillus fermentum br11 has been identified by using an antiserum raised against whole l. fermentum br11 cells. the first protein, rlp, is similar to the rib surface protein from streptococcus agalactiae, while the other protein, mlp, is similar to the mucus binding protein mub from lactobacillus reuteri. it was shown that multiple copies of mlp exist in the genome of l. fermentum br11. regions of rlp, ... | 2003 | 14532035 |
| quantitative real-time pcr analysis of fecal lactobacillus species in infants receiving a prebiotic infant formula. | the developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. generally, bifidobacterium and lactobacillus predominate the microbiota of breast-fed infants. in intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. however, the lactobacillus species distribution in breast-fed or formula-fed infants has not yet been determined in gre ... | 2006 | 16597930 |
| effect of lactobacillus isolates on the adhesion of pathogens to chicken intestinal mucus in vitro. | the aims of this study were to investigate in vitro the effects of lactobacillus isolates from a chicken on adhesion of pathogenic salmonella and escherichia coli to chicken intestinal mucus obtained from different intestinal regions. | 2006 | 16599990 |
| a fermentation assay to evaluate the effectiveness of antimicrobial agents on gut microflora. | the measurement of gas produced as a fermentation end product in vitro was correlated with absorbance as a measure of bacterial growth and was used as a rapid screening procedure to test the antimicrobial activity of certain essential oil and tannin secondary plant metabolites on gastrointestinal microorganisms from chickens. the assay was optimised using clostridium perfringens and lactobacillus fermentum, and tested in antimicrobial assays against c. perfringens; the minimum inhibitory concent ... | 2006 | 16632004 |
| protective role of supplement with foreign bifidobacterium and lactobacillus in experimental hepatic ischemia-reperfusion injury. | intestinal microflora play a crucial role in some severe liver diseases. the purpose of this study was to evaluate the effects of a lactobacillus strain and a bifidobacterium strain on ischemia-reperfusion (i/r) liver injury. | 2006 | 16677148 |
| antagonistic activity of probiotic lactobacilli and bifidobacteria against entero- and uropathogens. | to develop in vitro assays for comparing the antagonistic properties and anti-oxidative activity of probiotic lactobacillus and bifidobacterium strains against various entero- and urinary pathogens. | 2006 | 16696680 |
| screening for yeast with antibacterial properties from an ethanol distillery. | a general screening for the expression of antibacterial activity and non-flocculating type of yeast strains from must and fermented broth of alcohol distilleries was performed. from 60 strains only saccharomyces sp. m26 presented a inhibitory halo in lactobacillus fermentum culture and significant reduction in the culture turbidity (71%) and specific growth rate (56%) when compared to the control. freezing did not affect the antibacterial activity of the saccharomyces sp. m26 extract and heating ... | 2004 | 14643979 |
| assessment of in-line near-infrared spectroscopy for continuous monitoring of fermentation processes. | the application of nir in-line to monitor and control fermentation processes was investigated. determination of biomass, glucose, and lactic and acetic acids during fermentations of staphylococcus xylosus es13 was performed by an interactance fiber optic probe immersed into the culture broth and connected to a nir instrument. partial least squares regression (plsr) calibration models of second derivative nir spectra in the 700-1800 nm region gave satisfactory predictive models for all parameters ... | 2003 | 14656161 |
| probiotics for prevention of recurrent vulvovaginal candidiasis: a review. | vulvovaginal candidiasis (vvc) is a common infection affecting the quality of life of many women. probiotics have been investigated as possible agents for the prevention of recurrences of vvc. we reviewed the available literature. in some studies the development of vvc was associated with either a low number of lactobacilli in the vagina or with the presence of h2o2-non-producing vaginal lactobacilli, although there are a few studies not supporting these statements. in addition, in vitro studies ... | 2006 | 16790461 |
| microbiological characterization and probiotic potential of koko and koko sour water, african spontaneously fermented millet porridge and drink. | to identify and examine the diversity of predominant lactic acid bacteria (lab) in koko and koko sour water (ksw) from different ghanaian production sites with regard to pattern of fermentation (api 50 chl), genotype, antimicrobial activity, and resistance to low ph and bile salts. | 2004 | 14723700 |
| lactic acid bacteria isolated from canine faeces. | lactic acid bacteria (lab) were isolated and sequenced from the faeces of healthy dogs. five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine. | 2006 | 16834600 |
| probiotic properties of vaginal lactic acid bacteria to prevent metritis in cattle. | the isolation of bovine vaginal lactic acid bacteria (lab) and the screening of their beneficial properties to select those that could be used as probiotics in the prevention of bovine metritis were performed. | 2006 | 16834727 |
| effect of ph on lactobacillus fermentum growth, raffinose removal, alpha-galactosidase activity and fermentation products. | in this study, the behaviour of lactobacillus fermentum crl 722 and crl 251 were evaluated under different ph conditions (ph 6.0, 5.5, 5.0, 4.5) and without ph control. growth was similar under all conditions assayed except at ph 4.5. these microorganisms were able to eliminate raffinose, a nondigestible alpha-oligosaccharide (ndo) found in soy products, showing a consumption rate of 0.25 g l(-1) h(-1) (ph 6.0-5.0). the removal of raffinose was due to the high alpha-galactosidase (alpha-gal) act ... | 2004 | 14727095 |
| characteristics of the adhesion of pcc lactobacillus fermentum vri 003 to peyer's patches. | the characteristics of the adhesion of pcc lactobacillus fermentum vri 003 to peyer's patches was studied in vitro. the adhesion of l. fermentum 003 was strongly inhibited in the presence of d-mannose and methyl-alpha-d-mannoside although other carbohydrates tested, such as n-acetyl-glucosamine, d-galactose, d-glucose and l-fucose, did not affect the adhesion. lactobacillus fermentum 003 was shown to strongly attach to mannose immobilized on a surface using bsa, suggesting that l. fermentum 003 ... | 2006 | 16842353 |
| culture-independent analysis of the microbial composition of the african traditional fermented foods poto poto and dégué by using three different dna extraction methods. | the microbial composition of the traditional fermented foods poto poto (a maize dough from the rep. of congo) and dégué (a millet dough from burkina faso) was studied by a culture-independent approach using ttge to separate the amplified target v3 region of the 16s rrna gene from total microbial community, followed by dna sequencing and homology search. three different extraction methods were used. guanidium thiocyanate-based dna extraction provided better performance regarding purity and dna yi ... | 2006 | 16842876 |
| molecular identification of vaginal lactobacilli isolated from bulgarian women. | lactobacilli play an important role in maintaining the vaginal health of women. the development of suitable bacterial replacement therapies for the treatment of vaginosis requires knowledge of the vaginal lactobacilli species representation. the aim of this study was to identify at the species level vaginal lactobacillus isolates obtained from bulgarian women in childbearing age by using different molecular methods. twenty-two strains of lactobacilli isolated from vaginal samples were identified ... | 2006 | 16871423 |
| isolation and characterization of lactobacillus species inhibiting the formation of streptococcus mutans biofilm. | oral bacterium s11 was isolated from the saliva of young children without dental caries and with little or no visible supragingival plaque. the s11 strain showed 99.5% similarity with lactobacillus fermentum, and was identified on the basis of biochemical characteristics and a 16s rdna sequence. s11 strain and its culture supernatant significantly inhibited the formation of the insoluble glucan produced by streptococcus mutans ingbritt. s11 did not affect the multiplication of s. mutans ingbritt ... | 2004 | 15107076 |