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preliminary characterization of lactic acid bacteria isolated from zlatar cheese.isolation, characterization and identification of lactic acid bacteria (lab) from artisanal zlatar cheese during the ripening process and selection of strains with good technological characteristics.200718045397
presence of two lactobacillus and bifidobacterium probiotic strains in the neonatal ileum.the overall purpose of this study was to examine the lactobacilli and bifidobacteria microbiota in the human ileum at a very early stage of life. ileostomy effluents from two infants, taken at different time points, were plated on lactobacillus selective agar and cys-mrs containing mupirocin to select for bifidobacteria. in one case, a stool sample following ileostomy reversal was subsequently analyzed microbiologically. pulse-field gel electrophoresis and 16s rrna sequencing was used to investi ...200818059489
myd88 but not tlr2, 4 or 9 is essential for il-12 induction by lactic acid bacteria.although lactic acid bacteria (lab) affect the immune system, for example, having an anti-allergic effect, little is known about the actual mechanisms of immune modulation. toll-like receptors (tlrs) recognize conserved microbial molecular patterns, and are presumed to be involved in the recognition of lab. however, there are few detailed reports examining the relationships between tlr and lab. we measured here production of il-12, a cytokine considered to play an important role in anti-allergic ...200718071261
antifungal activity of lactobacillus paracasei ssp. tolerans isolated from a sourdough bread culture.lactic acid bacteria were isolated from four different sourdough bread cultures previously investigated for antifungal activity. a total of 116 isolates were obtained and screened for antifungal activity against a battery of molds. the most inhibitory isolate obtained was identified by api 50 chl and 16s ribosomal rna genotyping and found to be lactobacillus paracasei ssp. tolerans. this isolate completely inhibited the growth of fusarium proliferatum m 5689, m 5991 and fusarium graminearum r 40 ...200818077044
characterization of lactic acid bacteria isolated from bukuljac, a homemade goat's milk cheese.the bukuljac cheese is traditionally homemade cheese, produced from heat-treated goat's milk without the addition of any bacterial starter culture. the presence of lactic acid bacteria (lab) in bukuljac cheese has been analyzed by using a polyphasic approach including microbiological and molecular methods such as rep-pcr with (gtg)5 primer. lactobacillus paracasei subsp. paracasei represents a dominant strain in the microflora of analyzed cheese. out of 55 gram-positive and catalase-negative iso ...200818177967
three lactobacillus strains from healthy infant stool inhibit enterotoxigenic escherichia coli grown in vitro.enterotoxigenic escherichia coli (etec) are a major cause of sporadic diarrhea disease in humans, affecting mainly infants in developing countries and travelers from industrialized countries visiting tropical or subtropical areas. in this study, we screen the antagonistic activity by inoculating wells among etec agar cultures to assess inhibition zones created by the lactobacilli spent culture supernatant (scs) from healthy infant stool. only three isolates possessed antagonistic activity, acid ...200818182312
bioconversion of squid pen by lactobacillus paracasei subsp paracasei tku010 for the production of proteases and lettuce growth enhancing biofertilizers.a protease-producing bacterium, strain tku010, was isolated from infant vomited milk and identified as lactobacillus paracasei subsp. paracasei. a surfactant-stable protease, purified 64-fold from the third day culture supernatant to homogeneity in an overall yield of 11%, has a molecular weight of about 49,000. the enzyme degraded casein and gelatin, but did not degrade albumin, fibrin, and elastin. the enzyme activity was increased about 1.5-fold by the addition of 5mm ba(2+). however, fe(2+) ...200818187322
probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model.the transgenomic metabolic effects of exposure to either lactobacillus paracasei or lactobacillus rhamnosus probiotics have been measured and mapped in humanized extended genome mice (germ-free mice colonized with human baby flora). statistical analysis of the compartmental fluctuations in diverse metabolic compartments, including biofluids, tissue and cecal short-chain fatty acids (scfas) in relation to microbial population modulation generated a novel top-down systems biology view of the host ...200818197175
microbiological characterization of artisanal raschera pdo cheese: analysis of its indigenous lactic acid bacteria.the aim of this research was to study the bacterial populations involved in the production of artisanal raschera pdo cheese (italian maritime alps, northwest italy) in order to collect preliminary knowledge on indigenous lactic acid bacteria (lab). a total of 21 samples of raschera pdo cheese, collected from six dairy farms located in the production area, were submitted to microbiological analysis. lab were randomly isolated from m17 agar, mrs agar and kaa plates and identified by combining pcr ...200818206782
fecal microflora of greek healthy neonates.this study aimed to explore, in our geographical region, the development of intestinal microflora and the colonization patterns of lactic acid bacteria and bifidobacteria during the first three months of life and to investigate the effect of the mode of delivery. fecal specimens from 82 healthy, full-term infants were collected prospectively 4, 30 and 90 days after delivery and subcultured on nonselective and selective media. identification of isolates was performed by microbiological and molecu ...200818207437
probiotic crescenza cheese containing lactobacillus casei and lactobacillus acidophilus manufactured with high-pressure homogenized milk.high-pressure homogenization (hph) is one of the most promising alternatives to traditional thermal treatment of food preservation and diversification. its effectiveness on the deactivation of pathogenic and spoilage microorganisms in model systems and real food is well documented. to evaluate the potential of milk treated by hph for the production of crescenza cheese with commercial probiotic lactobacilli added, 4 types of cheeses were made: hph (from hph-treated milk), p (from pasteurized milk ...200818218736
ecological clarification of cheese whey prior to anaerobic digestion in upflow anaerobic filter.anaerobic digestion of cheese whey wastewaters (cw) was investigated in a system consisting of an ecological pretreatment followed by upflow anaerobic filter (uaf). the pretreatment was conducted to solve the inhibition problems during anaerobic treatment of cw caused by the amounts of fats, proteins and carbohydrates and to avoid the major problems of clogging in the reactor. the optimized ecological pretreatment of diluted cw induce removal yields of 50% of chemical oxygen demand (cod) and 60% ...200818242084
characterization of glutamate decarboxylase from a high gamma-aminobutyric acid (gaba)-producer, lactobacillus paracasei.gamma-aminobutyric acid (gaba) has several physiological functions in humans. we have reported that lactobacillus paracasei nfri 7415 produces high levels of gaba. to gain insight into the higher gaba-producing ability of this strain, we analyzed glutamate decarboxylase (gad), which catalyzes the decarboxylation of l-glutamate to gaba. the molecular weight of the purified gad was estimated to be 57 kda by sds-page and 110 kda by gel filtration, suggesting that gad forms the dimer under native co ...200818256502
microbial dynamics of castelmagno pdo, a traditional italian cheese, with a focus on lactic acid bacteria ecology.the dynamics of dominant microflora throughout the manufacture and ripening processes were evaluated in three batches of traditional castelmagno pdo cheese. milk, curd and cheese samples, at different stages during cheesemaking, were collected and subjected to culture-dependent and -independent analysis. traditional plating and genetic identification of lactic acid bacteria (lab) isolates, and pcr-dgge analysis of v1 region of 16s rrna gene were carried out. the collected samples were also monit ...200818272246
characterization of oral lactobacilli as potential probiotics for oral health.intestinal lactobacilli have been successfully used as probiotics to treat gastrointestinal disorders, but only limited data are available for the probiotic properties of oral lactobacilli to combat oral diseases. we aimed to characterize oral lactobacilli for their potential probiotic properties according to the international guidelines for the evaluation of probiotics, and to select potential probiotic strains for oral health.200818279182
phenotypic and molecular assessment of antimicrobial resistance in lactobacillus paracasei strains of food origin.antimicrobial resistance data in food-associated lactic acid bacteria (lab) such as lactobacilli are mostly based on nonstandardized methodologies and/or have been obtained for only a limited number of strains. this susceptibility study included a diverse collection of 115 isolates mainly of food origin originally identified as lactobacillus paracasei or lactobacillus casei. upon reidentification and removal of potential replicate isolates using repetitive dna element pcr fingerprinting, 65 geno ...200818326184
diversity and dynamics of lactobacilli populations during ripening of rdo camembert cheese.the diversity and dynamics of lactobacillus populations in traditional raw milk camembert cheese were monitored throughout the manufacturing process in 3 dairies. culture-dependent analysis was carried out on isolates grown on acidified de man - rogosa - sharpe agar and lactobacillus anaerobic de man rogosa sharpe agar supplemented with vancomycin and bromocresol green media. the isolates were identified by polymerase chain reaction - temperature gradient gel electrophoresis (pcr-tgge) and (or) ...200818388993
competition of lactobacillus paracasei with salmonella enterica for adhesion to caco-2 cells.competition of commensal and probiotic bacteria with pathogens for adhesion and colonization is one of the important protective mechanisms of gastrointestinal tract. in this study, we examined the ability of lactobacillus paracasei to inhibit the adhesion of pathogenic salmonella enterica to human colon adenocarcinoma caco-2 cells. caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively. in adhesion experiments, bacteria were added to the c ...200818401442
casitone-dependent transcriptional regulation of the prtp and prtm genes in the natural isolate lactobacillus paracasei subsp. paracasei.the prtp-prtm intergenic region of lactobacillus paracasei subsp. paracasei bghn 14 was cloned and sequenced. the nucleotide sequence of the prtp-prtm intergenic region in bghn 14, containing divergently orientated p(prtp) and p(prtp) promoters, was shorter by 35 bp in comparison with that in lactococci. the nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the bghn14. the activity of p(prtm) in l. lactis nz9000 was very l ...200718450219
effect of probiotic strains lactobacillus acidophilus lafti l10 and lactobacillus paracasei lafti l26 on systemic immune functions and bacterial translocation in mice.the immunostimulatory effects of lactobacillus acidophilus lafti l10 and lactobacillus paracasei lafti l26 were evaluated to determine their probiotic properties for functional food applications. mice were given oral doses of either l. acidophilus l10 or l. paracasei l26 (108 cfu/50 microl/day), and the effects on immune responses and bacterial translocation were assessed after the 14-day feeding trial. the proliferative responses of splenocytes to concanavalin a and lipopolysaccharide were sign ...200818468035
assessment of the safety, tolerance, and protective effect against diarrhea of infant formulas containing mixtures of probiotics or probiotics and prebiotics in a randomized controlled trial.probiotics and prebiotics are considered to be beneficial to the gastrointestinal health of infants.200818469260
coaggregation between and among human intestinal and oral bacteria.coaggregation is believed to facilitate the integration of new bacterial species into polymicrobial communities. the aim of this study was to investigate coaggregation between and among human oral and enteric bacteria. stationary phase cultures of 10 oral and 10 enteric species, chosen on the basis of numerical and ecological significance in their respective environments together with their ease of cultivation, were tested using a quantitative spectrophotometric coaggregation assay in all possib ...200818554305
the type strain of lactobacillus casei is atcc 393, atcc 334 cannot serve as the type because it represents a different taxon, the name lactobacillus paracasei and its subspecies names are not rejected and the revival of the name 'lactobacillus zeae' contravenes rules 51b (1) and (2) of the international code of nomenclature of bacteria. opinion 82.the judicial commission affirms that typification of lactobacillus casei is based on atcc 393, that atcc 334 is a member of a different taxon and that the publication rejecting the name lactobacillus paracasei (and its included subspecies) together with the revival of the name 'lactobacillus zeae' contravenes rules 51b (1) and (2) of the international code of nomenclature of bacteria.200818599731
top-down systems biology integration of conditional prebiotic modulated transgenomic interactions in a humanized microbiome mouse model.gut microbiome-host metabolic interactions affect human health and can be modified by probiotic and prebiotic supplementation. here, we have assessed the effects of consumption of a combination of probiotics (lactobacillus paracasei or l. rhamnosus) and two galactosyl-oligosaccharide prebiotics on the symbiotic microbiome-mammalian supersystem using integrative metabolic profiling and modeling of multiple compartments in germ-free mice inoculated with a model of human baby microbiota. we have sh ...200818628745
a factory-scale application of secondary adjunct cultures selected from lactic acid bacteria during puzzone di moena cheese ripening.the lactic acid populations of 2 seasonal puzzone di moena cheeses made from winter and summer raw cow's milk were characterized at different ripening times. lactic acid bacteria (lab) were isolated on selective media and subjected to genetic typing and identification. the species most frequently found during ripening were lactobacillus paracasei ssp. paracasei, lactobacillus plantarum, and pediococcus pentosaceus. the different strains recognized by random amplification of polymorphic dna-pcr w ...200818650274
polyphasic approach to bacterial dynamics during the ripening of spanish farmhouse cheese, using culture-dependent and -independent methods.we studied the dynamics of the microbial population during ripening of cueva de la magahá cheese using a combination of classical and molecular techniques. samples taken during ripening of this spanish goat's milk cheese in which lactococcus lactis and streptococcus thermophilus were used as starter cultures were analyzed. all bacterial isolates were clustered by using randomly amplified polymorphic dna (rapd) and identified by 16s rrna gene sequencing, species-specific pcr, and multiplex pcr. o ...200818658288
in vitro effects of selected synbiotics on the human faecal microbiota composition.synbiotics are recognized means of modulating gut microbiota composition and activities. however, whether synbiotics are superior to prebiotics and probiotics alone in moderating the gut microbiota towards a purportedly healthy composition has not been determined. eight selected synbiotics (short-chain fructooligosaccharides or fructooligosaccharides, each combined with one of four probiotics, lactobacillus fermentum me-3, lactobacillus plantarum wcfs1, lactobacillus paracasei 8700:2 or bifidoba ...200818673391
clinical trial on the efficacy of a new symbiotic formulation, flortec, in patients with irritable bowel syndrome: a multicenter, randomized study.efficacy of symbiotics in patients with irritable bowel syndrome (ibs) remains unknown.200818685503
microbial content and antibiotic susceptibility of bacterial isolates from yoghurts.a total of 83 dairy products form the greek market were examined for their bacterial populations and results were compared with the label information. the antibiotic susceptibility of the bacterial isolates was also examined. the status labeling of the yoghurts and the dairy desserts was found insufficient. the 89.8% of the cocci was found susceptible against all the 12 antibiotics examined. lactobacillus spp. strains exhibited some kind of resistance to one or more antibiotics. the isolates of ...200818686111
biodiversity of the microbial community in a spanish farmhouse cheese as revealed by culture-dependent and culture-independent methods.the microbial diversity within alberquilla cheese, made from a spontaneously fermented mixture of raw goats' and sheep's milk in the alpujarra mountains (granada, south-east spain), has been studied by the classical culturing method and also by molecular analysis of community dna. a collection of 206 isolates was obtained from the cheese on different selective/differential media, which were then re-grouped to 52 after randomly amplified polymorphic dna (rapd)-pcr analyses. isolates on man-rogosa ...200818692931
diversity of lactic acid bacteria in two flemish artisan raw milk gouda-type cheeses.pcr-denaturing gradient gel electrophoresis (pcr-dgge) was used to study the diversity of lactic acid bacteria (lab) in two flemish artisan raw milk gouda-type cheeses. in parallel, conventional culturing was performed. isolates were identified using (gtg)(5)-pcr and sequence analysis of 16s rrna and phes genes. discriminant analysis revealed some differences in overall lab diversity between the two batches and between the two cheeses. within each batch, the diversity of 8- and 12-week-old chees ...200818721684
time-dependent persistence of enhanced immune response by a potential probiotic strain lactobacillus paracasei subsp. paracasei ntu 101.the possible time-dependent role of lactic acid bacteria (lab) in immunomodulation was investigated in balb/c mice fed daily with lactobacillus paracasei subsp. paracasei ntu 101 (10(8) colony forming units) for 3, 6, and 9 weeks, and following feeding with lactobacillus-free food for a further 7 days. we observed up-regulation of the antigen-presenting ability of dendritic cells, and expression of natural killer group-2 d (nkg2d) molecules capable of trigger natural killer cell-mediated cytotox ...200818809220
effect of lactobacillus paracasei ncc2461 on antigen-specific t-cell mediated immune responses in aged mice.aging is associated with a reduced capacity to mount proper immune responses, in particular to vaccines. probiotic lactic acid bacteria may improve the immune status of the elderly; however, there is little evidence showing an effect of these bacteria on humoral and cellular immune responses. in the present study, the immunomodulatory capacity of the probiotic lactobacillus paracasei ncc2461 combined or not with a prebiotic composition, fos/inulin, was examined in aged mice. male c57bl/6j mice ( ...200818922048
sugar fermentation in probiotic bacteria--an in vitro study.food supplemented with probiotic bacteria is a rapidly growing sector of the market. the aim of the present study was to evaluate and compare the acid production of selected probiotic strains available in commercial products.200818954354
genetic screening of lactic acid bacteria of oenological origin for bacteriocin-encoding genes.a total of 330 lactic acid bacteria isolated from south african red wines during alcoholic and malolactic fermentations and 9 commercial malolactic bacteria starter cultures were screened for antimicrobial activity. of the entire screened isolates, 26 strains, belonging to the species lactobacillus plantarum, lactobacillus paracasei, lactobacillus hilgardii and oenococcus oeni, showed activity towards various wine-related and non-wine-related indicator strains. a pcr-based screening revealed the ...200818954734
diversity analysis of lactic acid bacteria in takju, korean rice wine.to investigate the lactic acid bacterial population in korean traditional rice wines, biotyping was performed using cell morphology and whole-cell protein pattern analysis by sdspage, and then the isolates were identified by 16s rrna sequencing analysis. based on the morphological characteristics, 103 lab isolates were detected in wine samples, characterized by whole-cell protein pattern analysis, and they were then divided into 18 patterns. by gene sequencing of 16s rrna, the isolates were iden ...200818955819
rep-pcr characterization and biochemical selection of lactic acid bacteria isolated from the delta area of egypt.samples of raw milk and traditional dairy products were collected from different rural areas in the delta region. 170 isolates from these products were identified using repetitive genomic element-pcr (rep-pcr) fingerprinting. the identified isolates were tested for efficiency of biomass production and separation, acidifying activity, autolytic and aminopeptidase properties, antagonistic activities and exopolysaccharide production. the obtained results revealed that the lactobacillus delbrueckii ...200918992953
potential of selected strains of lactic acid bacteria to induce a th1 immune profile.lactic acid bacteria (lab) might switch the th2 biased immune response in allergic patients towards a balanced th1/th2 immune profile, leading to amelioration of allergy. to select strains of lab that could be of potential application for foods in controlling allergy, 35 bacterial strains were screened in vitro using murine splenocytes and peritoneal exudate cells (pecs). streptococcus thermophilus ahu1838 (ferm ap-21009), and lactobacillus paracasei subsp. casei ahu1839 (ferm ap-21010) enhanced ...200818997432
intra-individual diversity and similarity of salivary and faecal microbiota.in the present study, polyphasic analysis [cultivation, combined with the fingerprinting of individual isolates, and denaturing gradient gel electrophoresis (dgge)] was applied to study whether similar features concerning the diversity and temporal stability of selected bacterial groups could be detected intra-individually in two different niches - the oral cavity and the colon - from ten adult volunteers consuming probiotics. the predominant bacterial microbiota, clostridium coccoides-eubacteri ...200819018030
evaluation of the probiotic potential of lactobacillus paracasei kw3110 based on in vitro tests and oral administration tests in healthy adults.lactobacillus paracasei kw3110 is a strain which has been reported to possess a strong antiallergic activity. since many lactic acid bacteria have shown to be useful as the probiotics, the possibility of this strain for use as a probiotic was studied. an in vitro test showed that this strain is strong enough to survive in the gastric juice. in addition, this strain showed exceptionally strong adherence to human intestinal epithelial cells, caco-2 and ht29 compared to normal l. paracasei strains. ...200819029768
antifungal activity of lactobacillus paracasei subsp. tolerans against fusarium proliferatum and fusarium graminearum in a liquid culture setting.lactobacillus paracasei subsp. tolerans, isolated from a traditional sourdough bread culture and previously shown to have antifungal activity against fusarium species, was tested for inhibition of growth of fusarium proliferatum m 5991 and m 5689 and f. graminearum r 4053 in a liquid medium setting. this isolate completely inhibited the growth of f. proliferatum m 5689 and m 5991 and f. graminearum r 4053, whereas such growth was not inhibited in the control in a supernatant agar plate assay. wh ...200819044263
antibacterial properties of five bonding agents.this study compared antibacterial properties of five bonding agents with that of a control, chlorhexidine (2.5%). products evaluated were the self-etch primers (-p) and adhesives (-a) of clearfil se bond (se-p; se-a) [kuraray dental], clearfil protect bond (pb-p; pb-a) [kuraray dental], optibond solo self-etch (os-p; os-a) [kerr] and the one-bottle products, self-etch clearfil tri-s bond (3s) [kuraray dental] and total-etch adper scotchbond 1 xt (xt) [3m espe].200819055102
suitability of high pressure-homogenized milk for the production of probiotic fermented milk containing lactobacillus paracasei and lactobacillus acidophilus.high pressure homogenization (hph) is one of the most promising alternatives to traditional thermal treatment for food preservation and diversification. in order to evaluate its potential for the production of fermented milks carrying probiotic bacteria, four types of fermented milks were manufactured from hph treated and heat treated (ht) milk with and without added probiotics. microbiological, physicochemical and organoleptic analyses were carried out during the refrigerated period (35 d at 4 ...200919121239
effect of the yeast and bacteria biomass on the microbiota in the rumen.this study aims at obtaining a probiotic product based on viable biomass from 6 yeast strains and 2 strains of lactic bacteria used for nutrition of animals. the strains are subjected to some resistance tests, at temperature, ph, pepsin, pancreatin and biliary salts so as to make obvious their viability. tests were done by comparison to the witness strain and respectively a protective solution based on mucin and casein. based on the resulted viabilities 2 products are formulated. their effect is ...200819137830
effect of supplement with lactic-acid producing bacteria on fatigue and physical activity in patients with chronic fatigue syndrome.disturbances in intestinal microbial ecology and in the immune system of the host have been implicated as a part of the pathogenesis in chronic fatigue syndrome. probiotic lactic acid producing bacteria have been shown to prevent and alleviate gastrointestinal disturbances and to normalize the cytokine profile which might be of an advantage for patients suffering from chronic fatigue syndrome. the aim of the study was to evaluate the effect of lactobacillus paracasei ssp. paracasei f19, lactobac ...200919171024
[probiotics and children: is an integration useful in allergic diseases?].recent studies report that some probiotic strains are able to improve allergic diseases. for this reason, we would verify tolerability and efficacy of a industrial preparation of lactobacillus paracasei (11,688; proge farm, italy) and lactobacillus salivarius (11,794; proge farm, italy) and value their "in vitro" immunomodulatory effect. we know that, after birth, there's a persistence of th2 immune response that predisposes to atopy, whereas commensal bacteria are able to induce a th1 immune re ...200819216203
atherosclerosis-preventing activity of lactic acid bacteria-fermented milk-soymilk supplemented with momordica charantia.in this study, the milk-soymilk and milk-soymilk supplemented with momordica charantia , a common oriental vegetable possessing medicinal activities, were fermented by lactic bacteria. the objective of this study was to investigate the effects of milk-soymilk and fermented milk-soymilk with or without m. charantia on atherosclerosis in hyperlipidemic hamsters. fermented 25% milk and 75% soymilk combinations, supplemented with 1% m. charantia solution, can improve the acceptability of the ferment ...200919216552
b-cell activation in patients with irritable bowel syndrome (ibs).patients with irritable bowel syndrome (ibs) may have a low grade immune activation. however, little is known about the properties of b cells of ibs patients. we therefore investigated activation level and antigen presenting phenotype of blood b cells of ibs patients. we also examined b-cell responses to lipopolysaccharide (lps) and probiotic bacteria. blood samples were obtained from 74 ibs patients and 30 healthy subjects. peripheral blood mononuclear cells were isolated and stimulated with lp ...200919222763
probiotics, critical illness, and methodologic bias.it has been proposed that probiotics can favorably influence the course of critically ill patients. to address this question, a limited systematic review was undertaken (medline search for articles published in english) to identify randomized, controlled trials that compared a group of critically ill patients taking probiotics with a group that did not. ten such trials, mostly with high risks of methodologic bias, were identified. when the data were combined, the probiotics did not appear to inf ...200919244148
characterization of low-molecular-weight antiyeast metabolites produced by a food-protective lactobacillus-propionibacterium coculture.we developed a ph-controlled batch fermentation process with separately immobilized cells of the protective coculture of lactobacillus paracasei subsp. paracasei sm20 and propionibacterium jensenii sm11 in supplemented whey permeate medium yielding cell-free supernatants with high antiyeast activity against candida pulcherrima and rhodotorula mucilaginosa. the antiyeast compounds were resistant to proteinase k and pronase e treatments and showed high heat resistance (121 degrees c for 15 min). d ...200819244902
further characterization of a bacteriocin produced by lactobacillus paracasei hl32.purification, identification and partial characterization of bacteriocin produced by lactobacillus paracasei hl32. it has been shown to have activity against porphyromonas sp.200919245409
acid production by oral strains of candida albicans and lactobacilli.both candida albicans and lactobacilli are common colonizers of carious lesions in children and adolescents. the purpose of this study is to compare the velocity of acid production between c. albicans and several lactobacillus species at different ph levels and concentrations of glucose. washed, pure resting-cell suspensions were obtained by culturing a total of 28 oral isolates comprising the species c. albicans, lactobacillus rhamnosus, lactobacillus paracasei paracasei, lactobacillus paracase ...200919246906
polyphasic study of microbial communities of two spanish farmhouse goats' milk cheeses from sierra de aracena.the microbial communities present in 2 different types of farmhouse goats' milk cheese from the aracena mountains (southwest spain), quesailla arochena (hard cheese) and torta arochena (soft cheese), have been studied using both culture-dependent and culture-independent techniques. all bacterial isolates were clustered by using randomly amplified polymorphic dna (rapd) and identified by 16s rrna gene sequencing, species-specific pcr and multiplex pcr. thus a total of 26 different species were id ...200919269572
modification of azo dyes by lactic acid bacteria.the ability of lactobacillus casei and lactobacillus paracasei to modify the azo dye, tartrazine, was recently documented as the result of the investigation on red coloured spoilage in acidified cucumbers. fourteen other lactic acid bacteria (lab) were screened for their capability to modify the food colouring tartrazine and other azo dyes of relevance for the textile industry.200919302317
tolerance and safety of lactobacillus paracasei ssp. paracasei in combination with bifidobacterium animalis ssp. lactis in a prebiotic-containing infant formula: a randomised controlled trial.the addition of probiotics to infant formula has been shown to be an efficient way to increase the number of beneficial bacteria in the intestine in order to promote a gut flora resembling that of breast-fed infants. the objective of the present study was to evaluate the safety and tolerance of a combination of two probiotic strains in early infancy. a group of 126 newborns were randomised to receive a prebiotic-containing starter formula supplemented with lactobacillus paracasei ssp. paracasei ...200919331702
beneficial preventive effects of gastric mucosal lesion for soy-skim milk fermented by lactic acid bacteria.in this study, lactobacillus paracasei subsp. paracasei ntu101 and lactobacillus plantarum ntu 102 were used as starter to ferment soy-skim milk, and the optimal mixing ratio was evaluated. the influence of lactic acid bacteria (lab)-fermented soy-skim milk on mucosal integrity in a gastric mucosal lesion rat model was also investigated. after 24 h, cell densities of l. paracasei subsp. paracasei ntu 101 and l. plantarum ntu 102 fermented in 75% soy milk and 25% milk (optimal condition) were 1.2 ...200919358530
probiotic properties of lactobacillus rhamnosus and lactobacillus paracasei isolated from human faeces.the possibility of using microbes to maintain health, and to prevent or treat disease is a topic as old as microbiology. the research of novel probiotic strains is important in order to satisfy the increasing request of the market and to obtain functional products in which the probiotic cultures are more active and with better probiotic characteristics than those already present on the market.200919365593
characterization of the tn916 conjugative transposon in a food-borne strain of lactobacillus paracasei.food-borne antibiotic-resistant lactic acid bacteria have received growing attention in the past few years. we have recently identified tetracycline-resistant lactobacillus paracasei in samples of milk and natural whey starter cultures employed in the manufacturing process of a typical italian fermented dairy product, mozzarella di bufala campana. in the present study, we have characterized at the molecular level the genetic context of tetracycline resistance determinants in these natural strain ...200919395574
diversity among lactobacillus paracasei phages isolated from a probiotic dairy product plant.to evaluate the phage diversity in the environment of a dairy industry which manufactures a product fermented with a probiotic strain of lactobacillus paracasei.200919486389
a mesocosm of lactobacillus johnsonii, bifidobacterium longum, and escherichia coli in the mouse gut.the relative contribution of competition and cooperation at the microbe-microbe level is not well understood for the bacteria constituting the gut microbiota. the high number and variability of human gut commensals have hampered the analysis. to get some insight into the question how so many different bacterial species can coexist in the mammalian gut, we studied the interaction between three human gut commensals (escherichia coli k-12, lactobacillus johnsonii ncc533, and bifidobacterium longum ...200919534605
transient acid-impairment of growth ability of oral streptococcus, actinomyces, and lactobacillus: a possible ecological determinant in dental plaque.dental plaque ph decreases to about 4 through bacterial fermentation of carbohydrates and this low ph is maintained for from several minutes to about an hour. repeated acidification causes demineralization of the tooth surface, resulting in caries formation. the acidification also influences plaque bacteria. severe acidification kills bacteria efficiently, while physiological acidification, the condition occurring in plaque, kills bacteria partially and may impair growth ability. we, therefore, ...200919572895
main microorganisms involved in the fermentation of ugandan ghee.ghee is widely produced from a traditional fermented butter-like product named mashita in western uganda. however, no detailed studies have been done to identify the microorganisms involved in mashita fermentation. the aim of this study was to identify the microorganisms present at the end of mashita ripening using culture-dependent and culture-independent techniques. the most commonly identified species of lactic acid bacteria (lab) in mashita using culture-dependent techniques were lactobacill ...200919577815
orally administered lactobacillus paracasei kw3110 induces in vivo il-12 production.lactic acid bacteria (lab) are popularly used as probiotics, and some strains of lab have anti-allergic functions in vivo. although in vitro studies show that lab modulate the t helper type (th) 1/th2 balance and inhibit ige secretion by inducing il-12, it is not known how lab regulates allergies in vivo. in this study, we evaluated in vivo il-12 production after oral administration of lactobacillus paracasei kw3110, a strain reported to improve allergies, to mice. orally administered kw3110 int ...200919584541
paracin 1.7, a bacteriocin produced by lactobacillus paracasei hd1.7 isolated from chinese cabbage sauerkraut, a traditional chinese fermented vegetable food.to purify and characterize bacteriocin produced by l. paracasei hd1.7.200919637568
[statistical methods to optimize immobilization and bacteriocin production by lactobacillus paracasei hd1.7].to enhance bacteriocin production by lactobacillus paracasei hd1.7 immobilized with calcium alginate gel by statistical methods.200919637577
variation in specificity of the prtp extracellular proteinases in lactococcus lactis and lactobacillus paracasei subsp. paracasei.comparison of cell-wall-bound extracellular proteinases (ceps) from lactobacillus paracasei (lbp) ssp. paracasei natural isolates bghn14, bgar75 and bgar76 with lactococcus lactis (lcl) ssp. cremoris wg2, in their action on alpha(s1)-, beta- and kappa-casein was done. the ceps of lbp strains were able to degrade alpha(s1)- and beta-caseins and their caseinolytic specificity depended on the type of buffer used. these ceps, compared with lcl wg2, differ in four amino acid residues in small segment ...200919649733
antagonistic activity of lactobacillus acidophilus ry2 isolated from healthy infancy feces on the growth and adhesion characteristics of enteroaggregative escherichia coli.enteroaggregative escherichia coli (eaggec) infection is an important cause of acute diarrhea, affecting children in developing countries and travelers visiting tropical or subtropical areas. three probiotics can exert bacteriostatic or bactericidal effects on human and animal intestinal pathogens, the efficiency of probiotics on eaggec infection remains unclear. in this study, the antagonistic activity of probiotic bacteria isolated from infant faeces was examined against several eaggec stains. ...200919655424
the effect of a new symbiotic formulation on plasma levels and peripheral blood mononuclear cell expression of some pro-inflammatory cytokines in patients with ulcerative colitis: a pilot study.during intestinal inflammation white blood cells are recruited from the blood, and they represent the major contributors to tissue perpetuation of inflammation via their production of chemokines and proinflammatory cytokines.200919694343
genome of a virulent bacteriophage lb338-1 that lyses the probiotic lactobacillus paracasei cheese strain.there is a lack of fundamental knowledge about the influence of bacteriophage on probiotic bacteria and other commensals in the gut. here, we present the isolation and morphological and genetic characterization of a virulent narrow-host-range bacteriophage, philb338-1. this phage was isolated from fresh sewage and was shown to infect the probiotic cheese strain lactobacillus paracasei nfbc 338. electron microscopy studies revealed that philb338-1 is a member of the myoviridae family, with an iso ...200919712732
antigen-presenting cells exposed to lactobacillus acidophilus ncfm, bifidobacterium bifidum bi-98, and bi-504 reduce regulatory t cell activity.the effect in vitro of six different probiotic strains including lactobacillus acidophilus ncfm, lactobacillus salivarius ls-33, lactobacillus paracasei subsp. paracasei ys8866441, lactobacillus plantarum lp-115, bifidobacterium bifidum bi-504 and bi-98 was studied on splenic enteroantigen-presenting cells (apc) and cd4(+)cd25(+) t-regulatory cells (tregs) in splenocyte-t cell proliferation assays.201019714743
assessment of cell surface properties and adhesion potential of selected probiotic strains.to evaluate the physicochemical cell surface and adhesive properties of selected probiotic strains for human use.200919725886
modulation of the immune response by probiotic strains in a mouse model of gluten sensitivity.probiotic strains play an important role in modulating activities in the gut-associated lymphoid tissue. elucidation of the mechanisms that mediate probiotic-driven immunomodulation may facilitate their therapeutic application for specific immune-mediated diseases or for prophylaxis. in this study, we explored the effect of different lactobacillus spp. and bifidobacterium lactis in transgenic mice expressing the human dq8 heterodimer, a hla molecule linked to celiac disease (cd). in vitro analys ...200919736022
1h nmr investigation on the role of sorbitol for the survival of lactobacillus paracasei ssp. paracasei in vacuum-dried preparations.the aim of this work was to study the effect of sorbitol as protective agent on the survival of the probiotic strain lactobacillus paracasei ssp. paracasei (f19) after vacuum drying.201019737343
lactic acid bacteria population in children diagnosed with human immunodeficiency virus.to determine the effect of trimethoprim/sulphamethoxazole treatment on the natural population of lactic acid bacteria in the intestinal tract and to determine if any of the strains developed resistance to antibiotics.200919751380
growth of lactobacillus paracasei a13 in argentinian probiotic cheese and its impact on the characteristics of the product.the growth capacity of probiotic lactobacillus paracasei a13, bifidobacterium bifidum a1 and l. acidophilus a3 in a probiotic fresh cheese commercialized in argentina since 1999 was studied during its manufacture and refrigerated storage at 5 degrees c and 12 degrees c for 60 days. additionally, viable cell counts for probiotic bacteria in the commercial product are reported for batch productions over the last 9 years. l. paracasei a13 grew a half log order at 43 degrees c during the manufacturi ...200919751954
effects of daily intake of lactobacillus paracasei strain kw3110 on japanese cedar pollinosis.japanese cedar pollinosis is an important contributor to allergic rhinitis in japan. lactobacillus may be useful as an immunomodulator and is used widely as a foodstuff. the purpose of the study was to examine the effects of daily intake of the lactobacillus paracasei strain kw3110 in patients with cedar pollinosis. the effects of daily intake of kw3110 in patients with cedar pollinosis were investigated in 126 patients who received kw3110 or a placebo in a double-blind study. the study began 1 ...200919772761
genetic screening of wine-related enzymes in lactobacillus species isolated from south african wines.the objective of this study was to investigate the presence of genes coding for enzymes of oenological relevance in wine lactobacillus strains isolated from south african grape and wine samples during the 2001 and 2002 harvest seasons.201019793136
genotypic characterization of non starter lactic acid bacteria involved in the ripening of artisanal bitto pdo cheese.bitto of valchiavenna, an artisanal italian cheese produced without the addition of any starter cultures, has been attributed a protected designation origin (pdo) cheese, but the strain composition of the natural microbial population colonizing this traditional dairy product is still unknown. to obtain preliminary information on the non starter lactic acid bacteria involved in its ripening, a total of 136 nslab isolates, randomly selected from mrs and m17 agar plates, were collected from three d ...200919810037
development of a pcr assay for the strain-specific identification of probiotic strain lactobacillus paracasei impc2.1.recent investigations clearly indicate that the probiotic bacterium lactobacillus paracasei impc2.1 can be incorporated into vegetables to obtain innovative probiotic foods whose marketing has been authorized by the italian ministry of health. in this study, strain impc2.1 was characterized at a molecular level in order to define its taxonomic position and to develop a pcr test for strain-specific identification. molecular methods, such as 16s rrna gene sequencing and multiplex pcr, have provide ...200919833402
clinical trial: the effects of a fermented milk containing three probiotic bacteria in patients with irritable bowel syndrome - a randomized, double-blind, controlled study.the effects of probiotic bacteria in ibs remain controversial.201019863495
association of beta-glucan endogenous production with increased stress tolerance of intestinal lactobacilli.the exopolysaccharide beta-glucan has been reported to be associated with many health-promoting and prebiotic properties. the membrane-associated glycosyltransferase enzyme (encoded by the gtf gene), responsible for microbial beta-glucan production, catalyzes the conversion of sugar nucleotides into beta-glucan. in this study, the gtf gene from pediococcus parvulus 2.6 was heterologously expressed in lactobacillus paracasei nfbc 338. when grown in the presence of glucose (7%, wt/vol), the recomb ...201019933353
combined effects of probiotic fermentation and high-pressure extraction on the antioxidant, antimicrobial, and antimutagenic activities of deodeok (codonopsis lanceolata).this study was designed to evaluate the combined effects of probiotic fermentation and high-pressure extraction (hpe) on the functional properties of codonopsis lanceolata. the ground c. lanceolata samples were anaerobically fermented with lactobacillus acidophilus adh, bifidobacterium longum b6, lactobacillus rhamnosus gg, or lactobacillus paracasei at 37 degrees c for 10 days and subjected to 500 mpa at 50 degrees c for 30 min. the extraction yields of c. lanceolata samples were noticeably inc ...201020000575
validation of the dorsal air pouch model to predict and examine immunostimulatory responses in the gut.to validate the use of the air pouch system to predict and examine early immune responses induced by the presumptive probiotics lactobacillus paracasei subsp. paracasei b112, dc205, dc215 and dc412 strains in the gut mucosa.201020002910
the effects of antimicrobials on endodontic biofilm bacteria.in the present study, confocal microscopy, a miniflow cell system, and image analysis were combined to test in situ the effect of antimicrobials and alkali on biofilms of enterococcus faecalis, lactobacillus paracasei, streptococcus anginosus, and streptococcus gordonii isolated from root canals with persistent infections.201020003938
isolation and functional characterization of a biosurfactant produced by lactobacillus paracasei.in this study, the crude biosurfactant produced by a lactobacillus paracasei strain isolated in a portuguese dairy industry was characterized. the minimum surface tension (41.8mn/m) and the critical micelle concentration (2.5mg/ml) obtained were found to be similar to the values previously reported for biosurfactants isolated from other lactobacilli. the biosurfactant was found to be stable to ph changes over a range from 6 to 10, being more effective at ph 7, and showed no loss of surface activ ...201020004557
susceptibility to tetracycline and erythromycin of lactobacillus paracasei strains isolated from traditional italian fermented foods.the aim of this study was to evaluate the susceptibility of 197 isolates of lactobacillus paracasei, isolated from italian fermented products coming from different geographical areas, to tetracycline and erythromycin, two antimicrobials widely used in clinical and animal therapy. isolation media were supplemented with antibiotics according to the microbiological breakpoints (bps) defined by european food safety authority (efsa). isolates were identified at the species level and were typed by rep ...201020005592
lactobacilli prevent hydroxy radical production and inhibit escherichia coli and enterococcus growth in system mimicking colon fermentation.to explore the effect of lactobacillus on redox state of colon chyme.201020059670
dietary supplementation with lactobacilli and bifidobacteria is well tolerated and not associated with adverse events during late pregnancy and early infancy.lactic acid bacteria and bifidobacteria are increasingly being administered to pregnant women and infants with the intention of improving health. although these organisms have a long record of safe use, it is important to identify any adverse effects in potentially vulnerable populations. in a randomized, double-blinded, placebo-controlled trial, we evaluated the safety of a bacterial dietary supplement for the prevention of atopy in infants. two strains of lactobacilli (lactobacillus salivarius ...201020089774
lactobacillus-mediated rna interference in nematode.we engineered lactobacillus paracasei to produce a dsrna that would trigger rnai-induced silencing of an essential gene in the nematode caenorhabditis elegans. the dsrna-expressing l. paracasei can be used in experiments conducted on culture plates and may also be used as an orally administrable dsrna carrier for humans and other mammals.201020129106
expression, purification and use of the soluble domain of lactobacillus paracasei beta-fructosidase to optimise production of bioethanol from grass fructans.microbial inulinases find application in food, pharmaceutical and biofuel industries. here, a novel lactobacillus paracasei beta-fructosidase was overexpressed as truncated cytosolic protein ((t)fosep) in escherichia coli. purified (t)fosep was thermostable (10-50 degrees c) with a ph optimum of 5; it showed highest affinity for bacterial levan (beta[2-6] linked fructose) followed by nystose, chicory inulin, 1-kestose (beta[2-1] linkages) and sucrose (k(m) values of 0.5, 15, 15.6, 49 and 398 mm, ...201020153640
antimicrobial and antiadhesive properties of a biosurfactant isolated from lactobacillus paracasei ssp. paracasei a20.the aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from lactobacillus paracasei ssp. paracasei a20 against several micro-organisms, including gram-positive and gram-negative bacteria, yeasts and filamentous fungi.201020184670
intestinal lactobacillus sp. is associated with some cellular and metabolic characteristics of blood in elderly people.the higher counts or particular groups (firmicutes/bacteroidetes) of intestinal microbiota are related to host metabolic reactions, supporting a balance of human ecosystem. we further explored whether intestinal lactobacilli were associated with some principal cellular and metabolic markers of blood in 38 healthy >65-year-old persons. the questionnaire, routine clinical and laboratory data of blood indices as much as the oxidized low-density lipoprotein (ox-ldl) and baseline diene conjugates in ...201020223288
probiotic table olives: microbial populations adhering on olive surface in fermentation sets inoculated with the probiotic strain lactobacillus paracasei impc2.1 in an industrial plant.this study reports the dynamics of microbial populations adhering on the surface of debittered green olives cv. bella di cerignola in fermentation sets inoculated with the probiotic strain lactobacillus paracasei impc2.1 in different brining conditions (4% and 8% (w/v) nacl) at room temperature and 4 degrees c. the probiotic strain successfully colonized the olive surface dominating the natural lab population and decreasing the ph of brines to <or=5.0 after 30 days until the end of fermentation. ...201020226556
m-rtlv agar, a novel selective medium to distinguish lactobacillus casei and lactobacillus paracasei from lactobacillus rhamnosus.we developed a novel selective medium, modified-rhamnose-2,3,5-triphenyltetrazolium chloride-lbs-vancomycin agar (m-rtlv agar), that utilizes the fermentability of l-rhamnose to distinguish lactobacillus casei and lactobacillus paracasei from lactobacillus rhamnosus. whereas l. casei and l. paracasei formed red colonies on the m-rtlv agar, l. rhamnosus formed either pink-toned colonies or white colonies with a red spot. an intervention study was conducted to confirm the capability of m-rtlv agar ...201020385416
influence of microfiltration and adjunct culture on quality of domiati cheese.the effects of microfiltration and pasteurization processes on proteolysis, lipolysis, and flavor development in domiati cheese during 2 mo of pickling were studied. cultures of starter lactic acid bacteria isolated from egyptian dairy products were evaluated in experimental domiati cheese for flavor development capabilities. in the first trial, raw skim milk was microfiltered and then the protein:fat ratio was standardized using pasteurized cream. pasteurized milk with same protein:fat ratio wa ...201020412894
identification of the bacterial biodiversity in koumiss by denaturing gradient gel electrophoresis and species-specific polymerase chain reaction.bacterial biodiversity in traditional koumiss fermented milk was studied by denaturing gradient gel electrophoresis (dgge). target dna bands were identified according to the reference species ladder, constructed in this study. comigrating bands present in the dgge profiles were resolved by species-specific pcr. the results revealed a novel bacterial profile and extensive bacterial biodiversity in koumiss. the dominant lactic acid bacteria included lactobacillus acidophilus, lactobacillus helveti ...201020412906
phenotypic and genetic heterogeneity of lactic acid bacteria isolated from "alheira", a traditional fermented sausage produced in portugal.the aim of this study was to evaluate the phenotypic and genetic heterogeneity of lactic acid bacteria (lab) isolated from "alheira", a fermented sausage produced in portugal. lab were identified to genus and species level by phenotypic characteristics, using genus or species-specific primers and sequencing of the gene encoding 16s rrna. two-hundred and eighty-three isolates were grouped into 14 species. lactobacillus plantarum was isolated from all sausages and enterococcusfaecalis from most of ...200920416703
effect of the administration of lactobacillus paracasei subsp. paracasei ntu 101 on peyer's patch-mediated mucosal immunity.the role of lactic acid bacteria in gut mucosal immunity was investigated by comparing the enhanced effects in the peyer's patches and spleen of balb/c mice fed daily with lactobacillus paracasei subsp. paracasei ntu 101 for 3 to 9 weeks. after feeding with lactobacillus, the percentage of cd4+ t cells in both peyer's patches and the spleen was significantly increased; however, expression of cd 154 molecules, which play a pivotal role in cell-to-cell communication, on cd4+ t cells and the percen ...201020417727
construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from lactobacillus paracasei subsp. paracasei bgsj2-8.a new shuttle-cloning vector, pa13, was constructed and successfully introduced into escherichia coli, lactobacillus and lactococcus strains. it showed high segregational and structural stability in all three hosts. the natural plasmid psj2-8 from l. paracasei subsp. paracasei bgsj2-8 was cloned into pa13 using bamhi to obtain the construct, pb5. sequencing and in silico analysis of pb5 revealed fifteen open reading frames (orf). plasmid psj2-8 harbours genes encoding the production of two bacte ...201020439125
growth-inhibition of hiochi bacteria in namazake (raw sake) by bacteriocins from lactic acid bacteria.the bacteriocins produced by lactococcus lactis subsp. lactis c101910 (c101910) and nbrc 12007 (nbrc 12007) were used to prevent the growth of sake spoiling hiochi bacteria (lactobacillus hilgardii, lactobacillus fructivorans, and lactobacillus paracasei) in namazake, which is raw (unpasteurized) sake. the bacteriocin concentrations required for decreasing the viable cell concentrations of l. hilgardii and l. fructivorans below the detection limit (1.0 x 10(2) cells/ml) in 24 h from the initial ...201020471596
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