| felled oil palm trunk as a renewable source for biobutanol production by clostridium spp. | this study aimed to convert felled oil palm trunk to biobutanol by clostridium spp. for efficient utilization of oil palm trunk, it was separated into sap and trunk fiber. the sap was used directly while the trunk fiber was hydrolyzed to fermentable sugars before use. among five clostridia strains screened, clostridium acetobutylicum dsm 1731 was the most suitable strain for butanol production from the sap without any supplementation of nutrients. it produced the highest amount of butanol (14.4 ... | 2013 | 23933028 |
| metabolic engineering of clostridium acetobutylicum for the enhanced production of isopropanol-butanol-ethanol fuel mixture. | butanol is considered as a superior biofuel, which is conventionally produced by clostridial acetone-butanol-ethanol (abe) fermentation. among abe, only butanol and ethanol can be used as fuel alternatives. coproduction of acetone thus causes lower yield of fuel alcohols. thus, this study aimed at developing an improved clostridium acetobutylicum strain possessing enhanced fuel alcohol production capability. for this, we previously developed a hyper abe producing bkm19 strain was further enginee ... | 2013 | 23606675 |
| production of acetone, butanol, and ethanol from biomass of the green seaweed ulva lactuca. | green seaweed ulva lactuca harvested from the north sea near zeeland (the netherlands) was characterized as feedstock for acetone, ethanol and ethanol fermentation. solubilization of over 90% of sugars was achieved by hot-water treatment followed by hydrolysis using commercial cellulases. a hydrolysate was used for the production of acetone, butanol and ethanol (abe) by clostridium acetobutylicum and clostridium beijerinckii. hydrolysate-based media were fermentable without nutrient supplementat ... | 2013 | 23201525 |
| simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by clostridium acetobutylicum atcc 824 engineered strains. | isopropanol represents a widely-used commercial alcohol which is currently produced from petroleum. in nature, isopropanol is excreted by some strains of clostridium beijerinckii, simultaneously with butanol and ethanol during the isopropanol butanol ethanol (ibe) fermentation. in order to increase isopropanol production, the gene encoding the secondary-alcohol dehydrogenase enzyme from c. beijerinckii nrrl b593 (adh) which catalyzes the reduction of acetone to isopropanol, was cloned into the a ... | 2012 | 22909015 |
| introducing a single secondary alcohol dehydrogenase into butanol-tolerant clostridium acetobutylicum rh8 switches abe fermentation to high level ibe fermentation. | | 2012 | 22742819 |
| [periodontal microbiota and microorganisms isolated from heart valves in patients undergoing valve replacement surgery in a clinic in cali, colombia]. | periodontitis is an infectious disease that affects the support tissue of the teeth and it is associated with different systemic diseases, including cardiovascular disease. microbiological studies facilitate the detection of microorganisms from subgingival and cardiovascular samples. | 2017 | 29373772 |
| renewable synthesis of n-butyraldehyde from glucose by engineered escherichia coli. | n-butyraldehyde is a high-production volume chemical produced exclusively from hydroformylation of propylene. it is a versatile chemical used in the synthesis of diverse c4-c8 alcohols, carboxylic acids, esters, and amines. its high demand and broad applications make it an ideal chemical to be produced from biomass. | 2017 | 29213330 |
| development of an oxygen-independent flavin mononucleotide-based fluorescent reporter system in clostridium beijerinckii and its potential applications. | clostridium beijerinckii is a predominant solventogenic clostridia with great attraction for renewable liquid biofuel and biochemical production. metabolic engineering and synthetic biology can be employed to engineer the strain toward desirable phenotypes. however, current limited information such as promoter strength and gene regulation may hinder the efficient engineering of the strain. to investigate genetic information and complex cellular bioprocesses of c. beijerinckii, an in vivo fluores ... | 2018 | 29158189 |
| engineering cofactor flexibility enhanced 2,3-butanediol production in escherichia coli. | enzymatic reduction of acetoin into 2,3-butanediol (2,3-bd) typically requires the reduced nicotinamide adenine dinucleotide (nadh) or its phosphate form (nadph) as electron donor. efficiency of 2,3-bd biosynthesis, therefore, is heavily influenced by the enzyme specificity and the cofactor availability which varies dynamically. this work describes the engineering of cofactor flexibility for 2,3-bd production by simultaneous overexpression of an nadh-dependent 2,3-bd dehydrogenase from klebsiell ... | 2017 | 29116429 |
| the draft genome sequence of clostridium beijerinckii njp7, a unique bacterium capable of producing isopropanol-butanol from hemicellulose through consolidated bioprocessing. | a wild type solventogenic clostridium beijerinckii njp7 capable of converting polysaccharides, such as hemicellulose, into butanol and isopropanol via a unique acetone-isopropanol-butanol (aib) pathway was isolated and characterized. this represents the first wild type isopropanol-butanol generating bacterium which could achieve butanol production directly from lignocellulose through consolidated bioprocessing (cbp). strain njp7 was isolated from decomposite soil from laoshan nature park, china, ... | 2017 | 29063966 |
| comparison of expression of key sporulation, solventogenic and acetogenic genes in c. beijerinckii nrrl b-598 and its mutant strain overexpressing spo0a. | the production of acetone, butanol and ethanol by fermentation of renewable biomass has potential to become a valuable industrial process. mechanisms of solvent production and sporulation involve some common regulators in some abe-producing clostridia, although details of the links between the pathways are not clear. in this study, we compare a wild-type (wt) clostridium beijerinckii nrrl b-598 with its mutant strain oespo0a, in which the gene encoding spo0a, an important regulator of both sporu ... | 2017 | 28990140 |
| evidence of mixotrophic carbon-capture by n-butanol-producer clostridium beijerinckii. | recent efforts to combat increasing greenhouse gas emissions include their capture into advanced biofuels, such as butanol. traditionally, biobutanol research has been centered solely on its generation from sugars. our results show partial re-assimilation of co2 and h2 by n-butanol-producer c. beijerinckii. this was detected as synchronous co2/h2 oscillations by direct (real-time) monitoring of their fermentation gasses. additional functional analysis demonstrated increased total carbon recovery ... | 2017 | 28986542 |
| ferric iron and extracellular electron shuttling increase xylose utilization and butanol production during fermentation with multiple solventogenic bacteria. | xylose is the second most abundant sugar derived from lignocellulose; it is considered less desirable than glucose for fermentation, and strategies that specifically increase xylose utilization in wild-type cells are goals for biofuel production. xylose consumption, butanol production, and hydrogen production increased in both clostridium beijerinckii and a novel solventogenic bacterium (strain dc-1) when anthraquinone-2,6,-disulfonate (aqds) or riboflavin were used as redox mediators to transfe ... | 2017 | 28963627 |
| biobutanol production from apple pomace: the importance of pretreatment methods on the fermentability of lignocellulosic agro-food wastes. | apple pomace was studied as a possible raw material for biobutanol production. five different soft physicochemical pretreatments (autohydrolysis, acids, alkalis, organic solvents and surfactants) were compared in a high-pressure reactor, whose working parameters (temperature, time and reagent concentration) were optimised to maximise the amount of simple sugars released and to minimise inhibitor generation. the pretreated biomass was subsequently subjected to a conventional enzymatic treatment t ... | 2017 | 28932978 |
| erratum to: improving isopropanol tolerance and production of clostridium beijerinckii dsm 6423 by random mutagenesis and genome shuffling. | | 2017 | 28914328 |
| conversion of food processing wastes to biofuel using clostridia. | this study aims to demonstrate the recycling of food processing wastes as a low cost-effective substrate for acetone - butanol - ethanol (abe) production. potato peels and cheese whey were utilized during fermentation with eight local clostridium strains in addition to the commercial strain, c. acetobutylicum atcc 824 for abe and organic acids production. from potato peels, clostridium beijerinckii asu10 produced the highest abe production (17.91 g/l) representing 61.3% butanol (10.98 g/l), 33.6 ... | 2017 | 28823884 |
| biobutanol production from brewer's spent grain hydrolysates by clostridium beijerinckii. | brewer's spent grain (bsg) is a promising feedstock for abe fermentation. sulfuric acid pretreatment of bsg at ph 1, 121°c and different solid loadings (5-15% w/w) was investigated. enzymatic hydrolysis and abe fermentation by clostridium beijerinckii dsm 6422 of non-washed and washed pretreated bsg were performed to compare monosaccharide release and butanol production. pretreatment at 15% w/w bsg resulted in higher availability of sugars in both the enzymatic hydrolysates and pretreatment liqu ... | 2017 | 28779668 |
| genomic, transcriptional, and phenotypic analysis of the glucose derepressed clostridium beijerinckii mutant exhibiting acid crash phenotype. | clostridium beijerinckii is a predominant solventogenic bacterium that is used for the abe fermentation. various c. beijerinckii mutants are constructed for desirable phenotypes. the c. beijerinckii mutant ba105 harboring a glucose derepression phenotype was previously isolated and demonstrated the enhanced amylolytic activity in the presence of glucose. despite its potential use, ba105 is not further characterized and utilized. therefore, the authors investigate fermentation phenotypes of ba105 ... | 2017 | 28762642 |
| enhanced robustness in acetone-butanol-ethanol fermentation with engineered clostridium beijerinckii overexpressing adhe2 and ctfab. | clostridium beijerinckii cc101 was engineered to overexpress aldehyde/alcohol dehydrogenase (adhe2) and coa-transferase (ctfab). solvent production and acid assimilation were compared between the parental and engineered strains expressing only adhe2 (cc101-sv4) and expressing adhe2, ald and ctfab (cc101-sv6). cc101-sv4 showed an early butanol production from glucose but stopped pre-maturely at a low butanol concentration of ∼6g/l. compared to cc101, cc101-sv6 produced more butanol (∼12g/l) from ... | 2017 | 28747008 |
| enhanced n-butanol production by clostridium beijerinckii mcmb 581 in presence of selected surfactant. | extractive butanol fermentation with non-ionic surfactant, a recently explored area, has shown promising results with several advantages but is relatively less investigated. this work reports the extractive fermentation with selected non-ionic surfactants (l62 and l62d) to enhance butanol production using a high-butanol producing strain (clostridium beijerinckii mcmb 581). biocompatibility studies with both the surfactants showed growth. higher concentrations of surfactant (>5%) affected the cel ... | 2017 | 28660448 |
| a novel glycosylated solution from dioscorea zingiberensis c.h. wright significantly improves the solvent productivity of clostridium beijerinckii. | the economics of bio-solvent production are largely dependent on the cost of the fermentation substrate. dioscorea zingiberensis c.h. wright (dzw), the main raw material used to produce saponin, contains 13-18% starch which can be directly saccharified to a saccharification liquid of dzw starch (slds) that contains abundant nutrients. in this study, the water-soluble micromolecule compounds in slds were quantified through 1h nmr. using slds as the substrate to conduct abe fermentation by clostri ... | 2017 | 28577480 |
| mono- and co-substrate utilization kinetics using mono- and co-culture of clostridium beijerinckii and clostridium saccharoperbutylacetonicum. | the effect of co-culturing c. beijerinckii and c. saccharoperbutylacetonicum for h2 production using mono- and co-substrates of glucose, starch, and cellulose was assessed. monod kinetic parameters (k, maximum specific substrate utilization rate; and ks, half-saturation constant) of the c. beijerinckii, c. saccharoperbutylacetonicum, and the co-culture were determined. co-cultures utilizing glucose competed for the substrate, but showed enhancement for utilizing starch. the maximum values for k ... | 2017 | 28554101 |
| hydrogen production and microbial kinetics of clostridium termitidis in mono-culture and co-culture with clostridium beijerinckii on cellulose. | cellulose utilization by hydrogen producers remains an issue due to the low hydrogen yields reported and the pretreatment of cellulose prior to fermentation requires complex and expensive steps. clostridium termitidis is able to breakdown cellulose into glucose and produce hydrogen. on the other hand, clostridium beijerinckii is not able to degrade cellulose but is adept at hydrogen production from glucose; therefore, it was chosen to potentially enhance hydrogen production when co-cultured with ... | 2017 | 28429329 |
| genome editing in clostridium saccharoperbutylacetonicum n1-4 with the crispr-cas9 system. | clostridium saccharoperbutylacetonicum n1-4 is well known as a hyper-butanol-producing strain. however, the lack of genetic engineering tools hinders further elucidation of its solvent production mechanism and development of more robust strains. in this study, we set out to develop an efficient genome engineering system for this microorganism based on the clustered regularly interspaced short palindromic repeats (crispr) and crispr-associated 9 (crispr-cas9) system. first, the functionality of t ... | 2017 | 28258147 |
| transcriptome analysis of clostridium beijerinckii adaptation mechanisms in response to ferulic acid. | clostridium beijerinckii 4693:int with high ferulic acid (fa) tolerance was engineered and characterized in our lab. in this study, the minimum inhibition concentrations of fa against c. beijerinckii ncimb 8052 (wild-type) and 4693:int were 1.0 and 1.5g/l, respectively; cell viability was 18.5% and 106.7%, respectively, in the presence of 0.5g/l fa. a comparative transcriptome analysis was carried out at two different growth stages to evaluate sensitivity to fa. genes that were differentially ex ... | 2017 | 28242360 |
| sigma factor regulated cellular response in a non-solvent producing clostridium beijerinckii degenerated strain: a comparative transcriptome analysis. | clostridium beijerinckii dg-8052, derived from ncimb 8052, cannot produce solvent or form spores, a phenomenon known as degeneration. to explore the mechanisms of degeneration at the gene level, transcriptomic profiles of the wild-type 8052 and dg-8052 strains were compared. expression of 5168 genes comprising 98.6% of the genome was assessed. interestingly, 548 and 702 genes were significantly up-regulated in the acidogenesis and solventogenesis phases of dg-8052, respectively, and mainly respo ... | 2017 | 28194137 |
| modulation of the acetone/butanol ratio during fermentation of corn stover-derived hydrolysate by clostridium beijerinckii strain ncimb 8052. | producing biobutanol from lignocellulosic biomass has shown promise to ultimately reduce greenhouse gases and alleviate the global energy crisis. however, because of the recalcitrance of a lignocellulosic biomass, a pretreatment of the substrate is needed which in many cases releases soluble lignin compounds (slcs), which inhibit growth of butanol-producing clostridia. in this study, we found that slcs changed the acetone/butanol ratio (a/b ratio) during butanol fermentation. the typical a/b mol ... | 2017 | 28130305 |
| reclassification of non-type strain clostridium pasteurianum nrrl b-598 as clostridium beijerinckii nrrl b-598. | the complete genome sequence of non-type strain clostridium pasteurianum nrrl b-598 was introduced last year; it is an oxygen tolerant, spore-forming, mesophilic heterofermentative bacterium with high hydrogen production and acetone-butanol fermentation ability. the basic genome statistics have shown its similarity to c. beijerinckii rather than the c. pasteurianum species. here, we present a comparative analysis of the strain with several other complete clostridial genome sequences. besides a 1 ... | 2017 | 28111164 |
| transcriptional analysis of degenerate strain clostridium beijerinckii dg-8052 reveals a pleiotropic response to caco3-associated recovery of solvent production. | degenerate clostridium beijerinckii strain (dg-8052) can be partially recovered by supplementing caco3 to fermentation media. genome resequencing of dg-8052 showed no general regulator mutated. this study focused on transcriptional analysis of dg-8052 and its response to caco3 treatment via microarray. the expressions of 5168 genes capturing 98.6% of c. beijerinckii ncimb 8052 genome were examed. the results revealed that with addition of caco3 565 and 916 genes were significantly up-regulated, ... | 2016 | 27966599 |
| enhanced solvent production by metabolic engineering of a twin-clostridial consortium. | the efficient fermentative production of solvents (acetone, n-butanol, and ethanol) from a lignocellulosic feedstock using a single process microorganism has yet to be demonstrated. herein, we developed a consolidated bioprocessing (cbp) based on a twin-clostridial consortium composed of clostridium cellulovorans and clostridium beijerinckii capable of producing cellulosic butanol from alkali-extracted, deshelled corn cobs (aecc). to accomplish this a genetic system was developed for c. cellulov ... | 2017 | 27794465 |
| butanol production by a clostridium beijerinckii mutant with high ferulic acid tolerance. | a mutant strain of clostridium beijerinckii, with high tolerance to ferulic acid, was generated using atmospheric pressure glow discharge and high-throughput screening of c. beijerinckii ncimb 8052. the mutant strain m11 produced 7.24 g/l of butanol when grown in p2 medium containing 30 g/l of glucose and 0.5 g/l of ferulic acid, which is comparable to the production from non-ferulic acid cultures (8.11 g/l of butanol). when 0.8 g/l of ferulic acid was introduced into the p2 medium, c. beijerinc ... | 2016 | 26201246 |
| oxygen stability in the new [fefe]-hydrogenase from clostridium beijerinckii sm10 (cba5h). | the newly isolated clostridium beijerinckii [fefe]-hydrogenase cba5h was characterized by fourier transform infrared spectroscopy coupled to enzymatic activity assays. this showed for the first time that in this enzyme the oxygen-sensitive active state hox can be simply and reversibly converted to the oxygen-stable inactive hinact state. this suggests that oxygen sensitivity is not an intrinsic feature of the catalytic center of [fefe]-hydrogenases (h-cluster), opening new challenging perspectiv ... | 2016 | 27749036 |
| characterization of a clostridium beijerinckii spo0a mutant and its application for butyl butyrate production. | spo0a is a master regulator that governs the metabolic shift of solventogenic clostridium species such as clostridium beijerinckii. its disruption can thus potentially cause a significant alteration of cellular physiology as well as metabolic patterns. to investigate the specific effect of spo0a disruption in c. beijerinckii, a spo0a mutant of c. beijerinckii was characterized in this study. in a batch fermentation with ph control at 6.5, the spo0a mutant accumulated butyrate and butanol up to 8 ... | 2017 | 27474812 |
| investigation of availability of a high throughput screening method for predicting butanol solvent -producing ability of clostridium beijerinckii. | currently, efficient screening methods for selection of desired bacterial phenotypes from large populations are not easy feasible or readily available due to the complicated physiological and metabolic networks of solventogenic clostridia. in this study, to contribute to the improvement of methods for predicting the butanol-producing ability of clostridium beijerinckii based on starch substrate, we further investigate a simple, visualization screening method for selecting target strains from mut ... | 2016 | 27448996 |
| engineering clostridium beijerinckii with the cbei_4693 gene knockout for enhanced ferulic acid tolerance. | a mutant strain of clostridium beijerinckii ncimb 8052, c. beijerinckii m11, which exhibited ferulic acid tolerance up to 0.9g/l, was generated using atmospheric pressure glow discharge and high-throughput screening. comparative genomic analysis revealed that this strain harbored a mutation of the cbei_4693 gene, which encodes a hypothetical protein suspected to be an nadph-dependent fmn reductase. after disrupting the cbei_4693 gene in c. beijerinckii ncimb 8052 using the clostron group ii intr ... | 2016 | 27164255 |
| enhanced isopropanol and n-butanol production by supplying exogenous acetic acid via co-culturing two clostridium strains from cassava bagasse hydrolysate. | the focus of this study was to produce isopropanol and butanol (ib) from dilute sulfuric acid treated cassava bagasse hydrolysate (sacbh), and improve ib production by co-culturing clostridium beijerinckii (c. beijerinckii) with clostridium tyrobutyricum (c. tyrobutyricum) in an immobilized-cell fermentation system. concentrated sacbh could be converted to solvents efficiently by immobilized pure culture of c. beijerinckii. considerable solvent concentrations of 6.19 g/l isopropanol and 12.32 g/ ... | 2016 | 27116556 |
| proteomic analysis to elucidate degeneration of clostridium beijerinckii ncimb 8052 and role of ca(2+) in strain recovery from degeneration. | degeneration of solventogenic clostridium strains is one of the major barriers in bio-butanol production. a degenerated clostridium beijerinckii ncimb 8052 strain (dg-8052) was obtained without any genetic manipulation. supplementation of caco3 to fermentation medium could partially recover metabolism of dg-8052 by more than 50 % increase of cell growth and solvent production. this study investigated the protein expression profile of dg-8052 and its response to caco3 treatment. compared with wt- ... | 2016 | 27021843 |
| evaluation of viability, metabolic activity and spore quantity in clostridial cultures during abe fermentation. | flow cytometry, in combination with fluorescent staining, was used to evaluate population heterogeneity in acetone-butanol-ethanol fermentation that was carried out with type strain clostridium beijerinckii ncimb 8052 and non-type c. pasteurianum nrrl b-598. a combination of propidium iodide (pi) and carboxyfluorescein diacetate (cfda), pi plus syto-9 and bis-oxonol (box) alone were employed to distinguish between active and damaged cells together with simultaneous detection of spores. these str ... | 2016 | 26862145 |
| improving isopropanol tolerance and production of clostridium beijerinckii dsm 6423 by random mutagenesis and genome shuffling. | random mutagenesis and genome shuffling was applied to improve solvent tolerance and isopropanol/butanol/ethanol (ibe) production in the strictly anaerobic bacteria clostridium beijerinckii dsm 6423. following chemical mutagenesis with n-methyl-n-nitro-n-nitrosoguanidine (ntg), screening of putatively improved strains was done by submitting the mutants to toxic levels of inhibitory chemicals or by screening for their tolerance to isopropanol (>35 g/l). suicide substrates, such as ethyl or methyl ... | 2016 | 26852409 |
| ex situ product recovery for enhanced butanol production by clostridium beijerinckii. | in situ butanol recovery fermentation has been intensively studied as an effective alternative to conventional butanol production, which is limited due to the cellular toxicity of butanol. however, the low biocompatibility of adsorbents often leads to failure of in situ recovery fermentations. in this study, clostridium beijerinckii ncimb 8052 was cultured in flasks without shaking and in situ recovery fermentation was performed by using an adsorbent l493. the amounts of acetone, butanol, and et ... | 2016 | 26846537 |
| enhanced butanol production by increasing nadh and atp levels in clostridium beijerinckii ncimb 8052 by insertional inactivation of cbei_4110. | clostridium beijerinckii is identified as a promising clostridium strain for industrialization of acetone and butanol (ab) fermentation. it has been reported that high reducing power levels are associated with high butanol yield. in this study, we regulated reducing power by blocking nad(p)h consumption in c. beijerinckii ncimb 8052. gene cbei_4110, encoding nadh-quinone oxidoreductase (nuog), is a subunit of the electron transport chain complex i. after inactivation of gene cbei_4110, the gener ... | 2016 | 26830101 |
| glutamate and histidine improve both solvent yields and the acid tolerance response of clostridium beijerinckii ncp 260. | this study aims to examine the effect of amino acid supplementation on solvent production by clostridium beijerinckii during the acetone-butanol fermentation and to determine whether amino acids are involved in the acid tolerance response (atr), which results in increased solvents. | 2016 | 26789025 |
| simultaneous glucose and xylose uptake by an acetone/butanol/ethanol producing laboratory clostridium beijerinckii strain se-2. | most butanol-producing strains of clostridium prefer glucose over xylose, leading to a slower butanol production from lignocellulose hydrolysates. it is therefore beneficial to find and use a strain that can simultaneously use both glucose and xylose. | 2016 | 26721235 |
| efficient butanol recovery from acetone-butanol-ethanol fermentation cultures grown on sweet sorghum juice by pervaporation using silicalite-1 membrane. | we investigated butanol recovery by pervaporation separation, using a silicalite-1 membrane, from batch cultures of butanol-producing clostridium beijerinckii sbp2 grown on sweet sorghum juice as a fermentation medium. the pervaporation system yielded 73% (w/v) butanol from intact feed cultures containing 1% (w/v) butanol, and had a butanol permeation flux of 11 g m(-2) h(-1). upon neutralization and activated charcoal treatment of the feed cultures, butanol yield and total flux increased to 82% ... | 2016 | 26718336 |
| production of butanol and isopropanol with an immobilized clostridium. | clostridium beijerinckii optinoii is a clostridium species that produces butanol, isopropanol and small amounts of ethanol. this study compared the performances of batch and continuous immobilized cell fermentations, investigating how media flow rates and nutritional modification affected solvent yields and productivity. in 96-h batch cultures, with 80 % of the 30 g l(-1) glucose consumed in synthetic media, solvent concentration was 9.45 g l(-1) with 66.0 % as butanol. in a continuous fermentat ... | 2016 | 26712323 |
| enhanced butanol production in a microbial electrolysis cell by clostridium beijerinckii ib4. | reducing power such as nadh is an essential factor for acetone/butanol/ethanol (abe) fermentation using clostridium spp. the objective of this study was to increase available nadh in clostridium beijerinckii ib4 by a microbial electrolysis cell (mec) with an electron carrier to enhance butanol production. first of all, a mec was performed without electron carrier to study the function of cathodic potential applying. then, various electron carriers were tested, and neutral red (nr)-amended cultur ... | 2016 | 26615415 |
| butanol production from food waste: a novel process for producing sustainable energy and reducing environmental pollution. | waste is currently a major problem in the world, both in the developing and the developed countries. efficient utilization of food waste for fuel and chemical production can positively influence both the energy and environmental sustainability. this study investigated using food waste to produce acetone, butanol, and ethanol (abe) by clostridium beijerinckii p260. | 2015 | 26380581 |
| feasibility of a facile butanol bioproduction using planetary mill pretreatment. | a facile butanol bioproduction process was developed using planetary milling, and pinus rigida wood waste as a model substrate for fermentable sugars. the use of planetary milling as the pretreatment eliminates the need for washing and transfer of the biomass prior to enzymatic hydrolysis. moreover, using this pretreatment process resulted in the production of only 0.072 ± 0.003 g/l soluble phenolic compounds, a concentration that was not inhibitory towards clostridium beijerinckii ncimb 8052. a ... | 2016 | 26372608 |
| inoculum optimization of clostridium beijerinckii for reproducible growth. | spore-forming solventogenic clostridium spp. are receiving renewed attention due to their butanol production abilities. however, there is an absence of literature describing the preparation of dense, vigorous and homogeneous seed cultures of clostridium spp., guaranteeing reproducibility during fermentation. therefore, we performed a series of growth experiments of clostridium beijerinckii ncimb 8052 and its offspring sa-1 to evaluate the influence of inoculum age (harvest time) on the subsequen ... | 2015 | 26363015 |
| draft genome sequence of clostridium beijerinckii ne1, clostridia from an enrichment culture obtained from australian subterranean termite, nasutitermes exitiosus. | the draft genome of clostridium beijerinckii strain ne1 was reconstructed from the metagenomic sequence of a mixed-microbial consortium that produced commercially significant quantities of hydrogen from xylan as a sole feedstock. the organism possesses relatively limited hemicellulolytic capacity and likely requires the action of other organisms to completely degrade xylan. | 2015 | 25908128 |
| analysis of clostridium beijerinckii ncimb 8052's transcriptional response to ferulic acid and its application to enhance the strain tolerance. | plant-based cellulose presents the best source of renewable sugars for biofuel production. however, the lignin associated with plant cellulose presents a hurdle as hydrolysis of this component leads to the production of inhibitory compounds, such as ferulic acid. | 2015 | 25904983 |
| electrochemical detoxification of phenolic compounds in lignocellulosic hydrolysate for clostridium fermentation. | lignocellulosic biomass is being preferred as a feedstock in the biorefinery, but lignocellulosic hydrolysate usually contains inhibitors against microbial fermentation. among these inhibitors, phenolics are highly toxic to butyric acid-producing and butanol-producing clostridium even at a low concentration. herein, we developed an electrochemical polymerization method to detoxify phenolic compounds in lignocellulosic hydrolysate for efficient clostridium fermentation. after the electrochemical ... | 2015 | 25863199 |
| a biorefining process: sequential, combinational lignocellulose pretreatment procedure for improving biobutanol production from sugarcane bagasse. | here, for the first time, we designed a sequential, combinatorial lignocellulose pretreatment procedure (sclpp) for microbial biofuel fermentation to reduce generation of microbial growth inhibitors and furthermore increase sugar yields. we tested this pretreatment process using sugarcane bagasse as substrate and assessed the effectiveness by analysis of biobutanol production through microbial clostridium beijerinckii ncimb 8052 conversion. our results showed that there were no inhibitory effect ... | 2015 | 25846185 |
| genome sequence of the solvent-producing clostridium beijerinckii strain 59b, isolated from staffordshire garden soil. | the genome sequence of the solvent-producing, spore-forming, saccharolytic, mesophilic bacterium clostridium beijerinckii strain 59b, isolated from staffordshire garden soil, was obtained via a combination of sequencing with the 454 and illumina platforms. this information will allow for metabolic engineering of a potentially industrially useful strain. | 2015 | 25792046 |
| total synthesis of clostrubin. | clostrubin is a potent antibiotic against methicillin- and vancomycin-resistant bacteria that was isolated from a strictly anaerobic bacterium clostridium beijerinckii in 2014. this polyphenol possesses a fully substituted arene moiety on its pentacyclic scaffold, which poses a considerable challenge for chemical synthesis. here we report the first total synthesis of clostrubin in nine steps (the longest linear sequence). a desymmetrization strategy is exploited based on the inherent structural ... | 2015 | 25759087 |
| comparative phenotypic analysis and genome sequence of clostridium beijerinckii sa-1, an offspring of ncimb 8052. | production of butanol by solventogenic clostridia is controlled through metabolic regulation of the carbon flow and limited by its toxic effects. to overcome cell sensitivity to solvents, stress-directed evolution methodology was used three decades ago on clostridium beijerinckii ncimb 8052 that spawned the sa-1 strain. here, we evaluated sa-1 solventogenic capabilities when growing on a previously validated medium containing, as carbon- and energy-limiting substrates, sucrose and the products o ... | 2013 | 24068240 |
| use of fluorescent staining and flow cytometry for monitoring physiological changes in solventogenic clostridia. | physiological changes in populations of clostridium beijerinckii and clostridium tetanomorphum were monitored by fluorescence staining and flow cytometry. to estimate the number of metabolically active cells in exponential growth, a combination of the dyes propidium iodide and carboxy fluorescein diacetate appeared to be a good choice for both species. during stationary phase, these stains did not reflect physiological changes sufficiently and therefore additional labeling with bis-(1,3-dibutylb ... | 2014 | 24211310 |
| effects of supplementary butyrate on butanol production and the metabolic switch in clostridium beijerinckii ncimb 8052: genome-wide transcriptional analysis with rna-seq. | butanol (n-butanol) has high values as a promising fuel source and chemical feedstock. biobutanol is usually produced by the solventogenic clostridia through a typical biphasic (acidogenesis and solventogenesis phases) acetone-butanol-ethanol (abe) fermentation process. it is well known that the acids produced in the acidogenic phase are significant and play important roles in the switch to solventogenesis. however, the mechanism that triggers the metabolic switch is still not clear. | 2013 | 24229082 |
| selection of clostridium spp. in biological sand filters neutralizing synthetic acid mine drainage. | in this study, three biological sand filter (bsf) were contaminated with a synthetic iron- [1500 mg l⁻¹ fe(ii), 500 mg l⁻¹ fe(iii)] and sulphate-rich (6000 mg l⁻¹ so₄²⁻) acid mine drainage (amd) (ph = 2), for 24 days, to assess the remediation capacity and the evolution of autochthonous bacterial communities (monitored by t-rflp and 16s rrna gene clone libraries). to stimulate bsf bioremediation involving sulphate-reducing bacteria, a readily degradable carbon source (glucose, 8000 mg l⁻¹) was i ... | 2014 | 24251832 |
| enhanced abiotic and biotic contributions to dechlorination of pentachlorophenol during fe(iii) reduction by an iron-reducing bacterium clostridium beijerinckii z. | a novel fe(iii) reducing bacterium, clostridium beijerinckii z, was isolated from glucose amended paddy slurries, and shown to dechlorinate pentachlorophenol (pcp). fifty percent of added pcp was removed by c. beijerinckii z alone, which increased to 83% in the presence of both c. beijerinckii z and ferrihydrite after 11 days of incubation. without c. beijerinckii z, the surface-bound fe(ii) also abiotically dechlorinated more than 40% of the added pcp. this indicated that the biotic dechlorinat ... | 2014 | 24370696 |
| process integration for simultaneous saccharification, fermentation, and recovery (ssfr): production of butanol from corn stover using clostridium beijerinckii p260. | a simultaneous saccharification, fermentation, and recovery (ssfr) process was developed for the production of acetone-butanol-ethanol (ab or abe), of which butanol is the main product, from corn stover employing clostridium beijerinckii p260. of the 86 g l(-1) corn stover provided, over 97% of the sugars were released during hydrolysis and these were fermented completely with an abe productivity of 0.34 g l(-1)h(-1) and yield of 0.39. this productivity is higher than 0.31 g l(-1)h(-1) when usin ... | 2014 | 24398150 |
| effect of phosphoric acid pretreatment of corncobs on the fermentability of clostridium beijerinckii tistr 1461 for biobutanol production. | corncobs pretreated with h2so4, hno3, and h3po4 were compared to evaluate the fermentation ability of clostridium beijerinckii tistr 1461 to produce biobutanol via acetone-butanol-ethanol (abe) fermentation. it was found that the hydrolysate from h3po4 pretreatment could be used as a substrate without any inhibitor removal methods. however, in terms of sugar yield, it gave the lowest total sugars in both pretreatment and enzymatic hydrolysis. response surface methodology was applied to optimize ... | 2015 | 24678653 |
| pretreatment of lignocellulosic biomass using fenton chemistry. | in an attempt to mimic white-rot fungi lignin degradation via in vivo fenton chemistry, solution phase fenton chemistry (10 g biomass, 176 mmol hydrogen peroxide and 1.25 mmol fe(2+) in 200 ml of water) was applied to four different biomass feedstocks. an enzymatic saccharification of fenton pretreated biomass showed an average 212% increase relative to untreated control across all four feedstocks (p<0.05, statistically significant). a microbial fermentation of the same fenton pretreated biomass ... | 2014 | 24759643 |
| discovery of clostrubin, an exceptional polyphenolic polyketide antibiotic from a strictly anaerobic bacterium. | genome mining of the strictly anaerobic bacterium clostridium beijerinckii, an industrial producer of solvents, revealed the presence of several cryptic gene clusters for secondary metabolite biosynthesis. to unearth its metabolic potential, a c. beijerinckii strain was cultured under various conditions, which led to the discovery of a deep purple pigment. this novel metabolite, named clostrubin (1), was isolated and its structure was fully elucidated. the pentacyclic polyphenol features a benzo ... | 2014 | 24827417 |
| glycerol supplementation of the growth medium enhances in situ detoxification of furfural by clostridium beijerinckii during butanol fermentation. | lignocellulose-derived microbial inhibitors such as furfural and 5-hydroxymethyl furfural adversely affect fermentation of lignocellulosic biomass hydrolysates to fuels and chemicals due to their toxicity on fermenting microbes. to harness the potential of lignocellulose as a cheap source of fermentable sugars, in situ detoxification of furfural and other lignocellulose-derived microbial inhibitors is essential. to enhance in situ detoxification and tolerance of furfural by clostridium beijerinc ... | 2014 | 24839212 |
| acetone-butanol-ethanol (abe) production by clostridium beijerinckii from wheat straw hydrolysates: efficient use of penta and hexa carbohydrates. | abe fermentation by clostridium beijerinckii of steam-exploded and ozonated wheat straw hydrolysates was investigated. in steam-exploded hydrolysates, highest yields of 0.40 g/g abe yield and 127.71 g abe/kg wheat straw were achieved when the whole slurry from the pretreatment was used. in ozonated hydrolysates, 0.32 g/g abe yield and 79.65 g abe/kg wheat straw were obtained from washed ozonated wheat straw. diverse effects were observed in steam explosion and ozonolysis of wheat straw which res ... | 2014 | 24983690 |
| artificial symbiosis for acetone-butanol-ethanol (abe) fermentation from alkali extracted deshelled corn cobs by co-culture of clostridium beijerinckii and clostridium cellulovorans. | butanol is an industrial commodity and also considered to be a more promising gasoline substitute compared to ethanol. renewed attention has been paid to solvents (acetone, butanol and ethanol) production from the renewable and inexpensive substrates, for example, lignocellulose, on account of the depletion of oil resources, increasing gasoline prices and deteriorating environment. limited to current tools for genetic manipulation, it is difficult to develop a genetically engineered microorganis ... | 2014 | 25023325 |
| chemocatalytic upgrading of tailored fermentation products toward biodiesel. | biological and chemocatalytic processes are tailored in order to maximize the production of sustainable biodiesel from lignocellulosic sugar. thus, the combination of hydrotalcite-supported copper(ii) and palladium(0) catalysts with a modification of the fermentation from acetone-butanol-ethanol to isopropanol-butanol-ethanol predictably produces higher concentrations of diesel-range components in the alkylation reaction. | 2014 | 25044817 |
| elucidating and alleviating impacts of lignocellulose-derived microbial inhibitors on clostridium beijerinckii during fermentation of miscanthus giganteus to butanol. | fermentation of liquid hot water (lhw) pretreated miscanthus giganteus (mg) by clostridium beijerinckii ncimb 8052 was investigated towards understanding the toxicity of lignocellulose-derived inhibitors to solventogenic clostridium species vis-à-vis butanol production. while c. beijerinckii ncimb 8052 did not grow in undiluted mg hydrolysate-based fermentation medium, supplementation of this medium with calcium carbonate enabled the growth of c. beijerinckii ncimb 8052 and production of butanol ... | 2014 | 25085743 |
| clostridium beijerinckii mutant obtained atmospheric pressure glow discharge generates enhanced electricity in a microbial fuel cell. | a clostridium beijerinckii mutant m13 was derived from c. beijerinckii ncimb 8052 by atmospheric pressure glow discharge. c. beijerinckii m13 generated a maximum output power density of 79.2 mw m(-2) and a maximum output voltage of 230 mv in a microbial fuel cell containing 1 g glucose l(-1) as carbon source and 0.15 g methyl viologen l(-1) as an electron carrier. | 2015 | 25179822 |
| enhanced butanol production by immobilized clostridium beijerinckii tistr 1461 using zeolite 13x as a carrier. | butanol production by cell immobilization onto porous materials-brick and zeolite 13x-was investigated using clostridium beijerinckii tistr 1461. characterization results of two materials were completed to evaluate their potential as an immobilization carrier. although zeolite has greater porosity than brick, it cannot be used for cell aggregation without treating with chemical. after immobilization, both materials can enhance butanol titers from 5.29 to 5.80g/l and 8.58g/l using brick and zeoli ... | 2014 | 25237776 |
| cellulolytic enzymes production by utilizing agricultural wastes under solid state fermentation and its application for biohydrogen production. | phanerochaete chrysosporium was evaluated for cellulase and hemicellulase production using various agricultural wastes under solid state fermentation. optimization of various environmental factors, type of substrate, and medium composition was systematically investigated to maximize the production of enzyme complex. using grass powder as a carbon substrate, maximum activities of endoglucanase (188.66 u/gds), exoglucanase (24.22 u/gds), cellobiase (244.60 u/gds), filter paperase (fpu) (30.22 u/gd ... | 2014 | 25374139 |
| a novel three-component system-based regulatory model for d-xylose sensing and transport in clostridium beijerinckii. | d-xylose is the most abundant fermentable pentose in nature and can serve as a carbon source for many bacterial species. since d-xylose constitutes the major component of hemicellulose, its metabolism is important for lignocellulosic biomass utilization. here, we report a six-protein module for d-xylose signaling, uptake and regulation in solvent-producing clostridium beijerinckii. this module consists of a novel 'three-component system' (a putative periplasmic abc transporter substrate-binding ... | 2015 | 25441682 |
| water-insoluble material from apple pomace makes changes in intracellular nad⁺/nadh ratio and pyrophosphate content and stimulates fermentative production of hydrogen. | apple pomace is one of the major agricultural residues in aomori prefecture, japan, and it would be useful to develop effective applications for it. as apple pomace contains easily fermentable sugars such as glucose, fructose and sucrose, it can be used as a feedstock for the fermentation of fuels and chemicals. we previously isolated a new hydrogen-producing bacterium, clostridium beijerinckii hu-1, which could produce h2 at a production rate of 14.5 mmol of h2/l/h in a fed-batch culture at 37 ... | 2015 | 25468418 |
| complete genome sequence of solvent-tolerant clostridium beijerinckii strain sa-1. | we report the complete genome sequence of clostridium beijerinckii sa-1, derived by directed evolution from c. beijerinckii ncimb 8052, selecting for enhanced solvent tolerance. this sequence allows for accurate placement of sa-1 as c. beijerinckii, permits functional analyses of mutant phenotypes, and suggests methods for distinguishing sa-1 from its parent. | 2014 | 25523769 |
| purification and characterization of a gh11 xylanase from biobutanol-producing clostridium beijerinckii g117. | most biobutanol-producing clostridium strains are unable to ferment polysaccharides such as cellulose and xylan due to the lack of hydrolyzing enzymes. in this study, we show that clostridium beijerinckii g117, a newly isolated biobutanol-producing strain, expresses xylanase enzyme in the presence of 1% beechwood xylan. the xylanase activity in the medium containing actively growing culture and 1% of beechwood xylan can reach up to 2.66 u/ml after 14 h of fermentation. using salting-out and size ... | 2015 | 25564206 |
| production of biobutanol from acid-pretreated corncob using clostridium beijerinckii tistr 1461: process optimization studies. | corncob is a potential feedstock in thailand that can be used for fermentable sugar production through dilute sulfuric acid pretreatment and enzymatic hydrolysis. to recover high amounts of monomeric sugars from corncob, the sulfuric pretreatment conditions were optimized by using response surface methodology with three independent variables: sulfuric acid concentration, temperature, and time. the highest response of total sugars, 48.84 g/l, was found at 122.78°c, 4.65 min, and 2.82% (v/v) h2so4 ... | 2016 | 25569768 |
| markerless chromosomal gene deletion in clostridium beijerinckii using crispr/cas9 system. | the anaerobic spore-forming, gram-positive, solventogenic clostridia are notorious for being difficult to genetically engineer. based on crispr/cas9 assisted homologous recombination, we demonstrated that clean markerless gene deletion from the chromosome can be easily achieved with a high efficiency through a single-step transformation in clostridium beijerinckii ncimb 8052, one of the most prominent strains for acetone, butanol and ethanol (abe) production. this highly efficient genome enginee ... | 2015 | 25680931 |
| allopurinol-mediated lignocellulose-derived microbial inhibitor tolerance by clostridium beijerinckii during acetone-butanol-ethanol (abe) fermentation. | in addition to glucans, xylans, and arabinans, lignocellulosic biomass hydrolysates contain significant levels of nonsugar components that are toxic to the microbes that are typically used to convert biomass to biofuels and chemicals. to enhance the tolerance of acetone-butanol-ethanol (abe)-generating clostridium beijerinckii ncimb 8052 to these lignocellulose-derived microbial inhibitory compounds (ldmics; e.g., furfural), we have been examining different metabolic perturbation strategies to i ... | 2015 | 25690312 |
| improving biohydrogen production using clostridium beijerinckii immobilized with magnetite nanoparticles. | in order to supplement the need for alternative energy resources within the near future, enhancing the production of biohydrogen with immobilized clostridium beijerinckii ncimb8052 was investigated. magnetite nanoparticles were functionalized, with chitosan and alginic acid polyelectrolytes using a layer-by-layer method, to promote bacterial attachment. cultivating c. beijerinckii with these nanoparticles resulted in a shorter lag growth phase and increased total biohydrogen production within 10 ... | 2015 | 25728446 |
| direct conversion of sugars and organic acids to biobutanol by non-growing cells of clostridium spp. incubated in a nitrogen-free medium. | several clostridium spp. were incubated in a nitrogen-free medium (non-growth medium) containing only butyric acid as a sole precursor for performing butanol production by non-growing cells. non-growing cells of clostridium spp., especially clostridium beijerinckii tistr 1461, could convert butyric acid to butanol via their sole solventogenic activity. this activity was further enhanced in the presence of glucose as a co-substrate. in addition to glucose, other monosaccharides (i.e., galactose a ... | 2013 | 23996124 |
| lignocellulosic hydrolysates and extracellular electron shuttles for h2 production using co-culture fermentation with clostridium beijerinckii and geobacter metallireducens. | a co-culture of clostridium beijerinckii and geobacter metallireducens with ah2qds produced hydrogen from lignocellulosic hydrolysates (biomass of miscanthus prepared by hydrothermal treatment with dilute acids). this co-culture system enhanced hydrogen production from lignocellulosic hydrolysates by improving substrate utilization and diminishing acetate accumulation, despite the presence of fermentation inhibitors in the hydrolysates. the improvements were greater for xylose-rich hydrolysates. ... | 2013 | 23994308 |
| development of a gene knockout system using mobile group ii introns (targetron) and genetic disruption of acid production pathways in clostridium beijerinckii. | clostridium beijerinckii is a well-known solvent-producing microorganism with great potential for biofuel and biochemical production. to better understand and improve the biochemical pathway to solvents, the development of genetic tools for engineering c. beijerinckii is highly desired. based on mobile group ii intron technology, a targetron gene knockout system was developed for c. beijerinckii in this study. this system was successfully employed to disrupt acid production pathways in c. beijer ... | 2013 | 23872562 |
| evaluation of recycling the effluent of hydrogen fermentation for biobutanol production: kinetic study with butyrate and sucrose concentrations. | butyrate in the effluent of hydrogen-producing bioreactor is a potential feed for biobutanol production. for recycling butyrate, this study investigated the kinetics of biobutanol production by clostridium beijerinckii nrrl b592 from different paired concentrations of butyrate and sucrose in a series of batch reactors. results show that the lag time of butanol production increased with higher concentration of either sucrose or butyrate. in regression analyses, the maximum specific butanol produc ... | 2013 | 23866171 |
| wood pulp as an immobilization matrix for the continuous production of isopropanol and butanol. | the study was focused on developing a continuous method to produce an alcohol mixture suitable to be used as a gasoline supplement. the immobilized column reactor with wood pulp fibers was successfully used for the continuous production of butanol and isopropanol using clostridium beijerinckii dsm 6423. a sugar mixture (glucose, mannose, galactose, arabinose and xylose) representing lignocellulose hydrolysate was used as a substrate for the production of solvents. the effect of dilution rate on ... | 2013 | 23184175 |
| butanol production from hemicellulosic hydrolysate of corn fiber by a clostridium beijerinckii mutant with high inhibitor-tolerance. | a clostridium beijerinckii mutant rt66 with considerable inhibitor-tolerance obtained by continuous culture was used for butanol production from non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (sahhc). in fed-batch fermentation, 1.8l of diluted sahhc containing 10 g/l of reducing sugar was provided during the acidogenic phase and 0.2l of concentrated sahhc containing 300 g/l of reducing sugar was provided during the solventogenic phase. the mutant produc ... | 2013 | 22985825 |
| draft genome sequence of butanol-acetone-producing clostridium beijerinckii strain g117. | a recently discovered wild-type strain, clostridium beijerinckii g117, is unique in producing butanol and acetone but negligible amounts of ethanol, unlike previously identified acetone-butanol-ethanol (abe)-generating microbes. here we report the draft genome sequence of strain g117 (5,806,675 bp; gc content, 29.7%) and the novel findings obtained from its genome annotations. | 2012 | 22965093 |
| interactions between clostridium beijerinckii and geobacter metallireducens in co-culture fermentation with anthrahydroquinone-2, 6-disulfonate (ah2qds) for enhanced biohydrogen production from xylose. | to enhance biohydrogen production, clostridium beijerinckii was co-cultured with geobacter metallireducens in the presence of the reduced extracellular electron shuttle anthrahydroquinone-2, 6-disulfonate (ah(2)qds). in the co-culture system, increases of up to 52.3% for maximum cumulative hydrogen production, 38.4% for specific hydrogen production rate, 15.4% for substrate utilization rate, 39.0% for substrate utilization extent, and 34.8% for hydrogen molar yield in co-culture fermentation wer ... | 2013 | 22886601 |
| butanol production from wood pulping hydrolysate in an integrated fermentation-gas stripping process. | wood pulping hydrolysate (wph) containing mainly xylose and glucose as a potential substrate for acetone-butanol-ethanol (abe) fermentation was studied. due to the inhibitors present in the hydrolysate, several dilution levels and detoxification treatments, including overliming, activated charcoal adsorption, and resin adsorption, were evaluated for their effectiveness in relieving the inhibition on fermentation. detoxification using resin and evaporation was found to be the most effective metho ... | 2013 | 23827441 |
| enhanced butanol production by coculture of clostridium beijerinckii and clostridium tyrobutyricum. | cocultures of clostridium beijerinckii and clostridium tyrobutyricum in free-cell and immobilized-cell fermentation modes were investigated as a means of enhancing butanol production. the immobilized fermentation was performed in a fibrous-bed bioreactor (fbb). the results demonstrated that two-strain coculture significantly enhanced butanol production, yield and volumetric productivity compared with those in pure culture with or without butyric acid. further, continuous immobilized-cell cocultu ... | 2013 | 23819976 |
| acetone-butanol-ethanol (abe) production in fermentation of enzymatically hydrolyzed cassava flour by clostridium beijerinckii ba101 and solvent separation. | cassava constitutes an abundant substrate in tropical regions. the production of butanol in abe fermentation by clostridium beijerinckii ba101 using cassava flour (cf) was scaled-up to bioreactor level (5 l). optimized fermentation conditions were applied; that is, 40℃, 60 g/l cf, and enzymatic pretreatment of the substrate. the batch fermentation profile presented an acidogenic phase for the first 24 h and a solventogenic phase afterwards. an average of 37.01 g/l abe was produced after 83 h, wi ... | 2013 | 23727799 |
| identification of clostridium beijerinckii, cl. butyricum, cl. sporogenes, cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex pcr assay. | late blowing, caused by the outgrowth of clostridial spores present in raw milk and originating from silage, can create considerable product loss, especially in the production of hard and semi-hard cheeses. the conventional method for the isolation of clostridium spp. from cheeses with late-blowing symptoms is very complicated and the identification of isolates is problematic. the aim of this work was the development of a multiplex pcr method for the detection of the main dairy-related clostridi ... | 2012 | 22850580 |
| butanol production from corncob residue using clostridium beijerinckii ncimb 8052. | to determine whether corncob residue (ccr) could be a good substrate for butanol production. | 2012 | 22738279 |
| microbial production of a biofuel (acetone-butanol-ethanol) in a continuous bioreactor: impact of bleed and simultaneous product removal. | acetone butanol ethanol (abe) was produced in an integrated continuous one-stage fermentation and gas stripping product recovery system using clostridium beijerinckii ba101 and fermentation gases (co(2) and h(2)). in this system, the bioreactor was fed with a concentrated sugar solution (250-500 g l(-1) glucose). the bioreactor was bled semi-continuously to avoid accumulation of inhibitory chemicals and products. the continuous system was operated for 504 h (21 days) after which the fermentation ... | 2013 | 22729675 |
| acetone, butanol, and ethanol production from cane molasses using clostridium beijerinckii mutant obtained by combined low-energy ion beam implantation and n-methyl-n-nitro-n-nitrosoguanidine induction. | in order to obtain mutant strains showing higher solvent tolerance and butanol production than those of wild-type strains, the butanol-producing strain clostridium beijerinckii l175 was subjected to mutagenesis using a combined method of low-energy ion beam implantation and n-methyl-n-nitro-n-nitrosoguanidine induction. with this effort, mutant strain mut3 was isolated. when it was used for butanol fermentation in p2 medium, the production of butanol was 15.8±0.7 g/l 46% higher than the wild-typ ... | 2013 | 23587827 |
| "in situ" removal of isopropanol, butanol and ethanol from fermentation broth by gas stripping. | in this study, the removal of ibe from aqueous solutions by gas stripping has been characterized. the effect of one or more components in the solution on the kinetics of the separation has been studied, both at 37°c and at 70°c. gas stripping has been applied to batch, repeated batch and continuous cultures of clostridium beijerinckii grown on a glucose/xylose mixed sugar substrate mimicking lignocellulosic hydrolysates, with the aim of finding optimal conditions for a stable ibe-producing cultu ... | 2013 | 23584415 |
| butanol production from acid hydrolyzed corn fiber with clostridium beijerinckii mutant. | sulfuric acid treated corn fiber hydrolysate (sacfh) inhibited cell growth and the production of butanol (4.7±0.2 g/l) by clostridium beijerinckii ib4 in p2 medium. optimal medium components were determined using fractional factorial design. nh4hco3, feso4·7h2o and caco3 were demonstrated to be significant components in the production of butanol. the box-behnken design and a corresponding quadratic model were used to predict medium components (nh4hco3 1.96 g/l, feso4·7h2o 0.26 g/l and caco3 3.15 ... | 2013 | 23305897 |
| metabolic engineering of d-xylose pathway in clostridium beijerinckii to optimize solvent production from xylose mother liquid. | clostridium beijerinckii is an attractive butanol-producing microbe for its advantage in co-fermenting hexose and pentose sugars. however, this clostridium strain exhibits undesired efficiency in utilizing d-xylose, one of the major building blocks contained in lignocellulosic materials. here, we reported a useful metabolic engineering strategy to improve d-xylose consumption by c. beijerinckii. gene cbei2385, encoding a putative d-xylose repressor xylr, was first disrupted in the c. beijerincki ... | 2012 | 22677452 |
| genome-wide dynamic transcriptional profiling in clostridium beijerinckii ncimb 8052 using single-nucleotide resolution rna-seq. | clostridium beijerinckii is a prominent solvent-producing microbe that has great potential for biofuel and chemical industries. although transcriptional analysis is essential to understand gene functions and regulation and thus elucidate proper strategies for further strain improvement, limited information is available on the genome-wide transcriptional analysis for c. beijerinckii. | 2012 | 22433311 |
| time-resolved tryptophan fluorescence in flavodoxins. | the time-resolved fluorescence characteristics of tryptophan in flavodoxins isolated from the bacteriadesulfovibrio gigas, desulfovibrio vulgaris, clostridium beijerinckii, andmegasphaera elsdenii were examined. the fluorescence decays were recorded using pulsed synchrotron radiation as the excitation source and time-correlated single-photon counting in detection. the results were analyzed as lifetime distributions using the maximum entropy method. comparison of the fluorescence decays of normal ... | 1995 | 24226911 |