| a comparison of the lethal and hemolytic toxins of clostridium histolyticum. | | 1952 | 13006411 |
| the activity of clostridium histolyticum proteinase on synthetic substrates. | | 1953 | 13031635 |
| the haemolysin of clostridium histolyticum. | | 1953 | 13106224 |
| [studies on the properties of clostridium histolyticum and clostridium septicum antisera]. | | 1953 | 13124941 |
| separation of proteolytic enzymes of clostridium histolyticum. | | 1954 | 13223777 |
| [the proteolytic enzymes of clostridium histolyticum; influence of complexon iii and of calcium on the stability of two of these enzymes]. | | 1955 | 13260245 |
| [ammonia production and toxicity of clostridium histolyticum cultured in a gelatinated medium]. | | 1956 | 13363038 |
| pharmacologically-active polypeptide formed from blood globulin by a cysteine-activated protease from clostridium histolyticum. | | 1956 | 13373851 |
| [not available]. | | 1956 | 13395010 |
| exopeptidases of clostridium histolyticum. | | 1957 | 13445228 |
| nutritional studies with clostridium histolyticum. | | 1957 | 13462971 |
| [purification and chemical properties of anatoxin of clostridium histolyticum]. | | 1957 | 13499480 |
| effects of clostridium histolyticum enzymes upon burned and unburned tissues in vitro. | | 1958 | 13521674 |
| new proteolytic enzymes from clostridium histolyticum filtrates. | | 1958 | 13525621 |
| the cysteine-inactivated proteinase (alpha-antigen) of clostridium histolyticum. | | 1958 | 13525622 |
| serological evidence for a specific clostridium histolyticum gelatinase. | | 1958 | 13525623 |
| clostridium histolyticum collagenase: its purification and properties. | | 1958 | 13534676 |
| [studies on clostridium histolyticum anatoxins using zone electrophoresis and counter diffusion on agar]. | | 1959 | 13636341 |
| [study of immunological phenomena by means of tissue culture. iv. cytopathogenic effects of clostridium histolyticum toxin]. | | 1959 | 13658453 |
| studies on the collagenases of clostridium histolyticum. | | 1959 | 13661949 |
| the use of clostridium histolyticum enzymes in the treatment of experimental third degree burns. | | 1959 | 13675991 |
| [research on the odors emitted by anaerobic bacteria. iii. clostridium histolyticum]. | | 1961 | 13701340 |
| [clostripain, a protease from clostridium histolyticum. 2. specificity]. | | 1960 | 13708985 |
| [on proteins and their decomposition products. 18. the hydrolysis of synthetic and natural substrates by collagenase from clostridium histolyticum]. | | 1960 | 13714094 |
| [clostripain, a protease from clostridium histolyticum. 1. purification activation by thiols]. | | 1960 | 13758027 |
| electron microscopic studies on ultrathin sections of spores of clostridium tetani and clostridium histolyticum, with special reference to sporulation and spore germination process. | | 1960 | 13774854 |
| factors affecting the elaboration by clostridium histolyticum of proteinases capable of debriding third degree burn eschars on guinea pigs. | berman, sanford (walter reed army institute of research, washington, d. c.), joseph p. lowenthal, marion e. webster, patricia l. altieri, and raymond b. gochenour. factors affecting the elaboration by clostridium histolyticum of proteinases capable of debriding third degree burn eschars on guinea pigs. j. bacteriol. 82:582-588. 1961.-a comparison of three strains of clostridium histolyticum grown in two different media indicated that the h-4 strain produced the greatest yield of enzyme(s) capabl ... | 1961 | 13867979 |
| large scale purification of proteinases from clostridium histolyticum filtrates. | conklin, david a. (walter reed army institute of research, washington, d. c.), marion e. webster, patricia l. altieri, sanford berman, joseph p. lowenthal, and raymond b. gochenour. large scale purification of proteinases from clostridium histolyticum filtrates. j. bacteriol. 82:589-594. 1961.-a method for the large scale preparation and partial purification of clostridium histolyticum proteinases by fractional precipitation with ammonium sulfate is described. conditions for adequate separation ... | 1961 | 13880849 |
| [experimental inoculation of the chick embryo with clostridium histolyticum]. | | 1961 | 13884989 |
| debridement of burns on various animal species by clostridium histolyticum proteinases. | | 1962 | 13899369 |
| [stability behavior of collagenase from clostridium histolyticum]. | | 1963 | 13938916 |
| the interactions between clostridium septicum and clostridium histolyticum toxins and antitoxins. | | 1962 | 13983973 |
| enzymatic debridement of third-degree burns on guinea pigs by clostridium histolyticum proteinases. | webster, marion e. (walter reed army institute of research, washington, d. c.), patricia l. altieri, david a. conklin, sanford berman, joseph p. lowenthal, and raymond b. gochenour. enzymatic debridement of third-degree burns on guinea pigs by clostridium histolyticum proteinases. j. bacteriol. 83:602-608. 1962.-an attempt has been made to correlate in vitro activities of clostridium histolyticum h-4 proteinases, as measured against azocasein, azocoll, gelatin, and collagen, with their ability t ... | 1962 | 14005493 |
| multiplicity of clostridium histolyticum collagenases. | | 1964 | 14240635 |
| debridement of experimental burns with clostridium histolyticum h-4 enzymes. | | 1960 | 14399293 |
| [on proteins and their reduction products. xvi. on the specificity of collagenase from clostridium histolyticum and its application to the determination of the primary structure of collagen. (preliminary report)]. | | 1959 | 14401656 |
| toxicity of enzymes from clostridium histolyticum. | | 1960 | 14403502 |
| protease inhibitors: synthesis of bacterial collagenase and matrix metalloproteinase inhibitors incorporating succinyl hydroxamate and iminodiacetic acid hydroxamate moieties. | a series of succinyl hydroxamates/bishydroxamates as well as a new structural type of matrix metalloproteinase (mmp)/bacterial protease (bp) inhibitors, incorporating iminodiacetic (ida) hydroxamate/bishydroxamate moieties, has been synthesized and tested for interaction with four vertebrate proteases, mmp-1, mmp-2, mmp-8 and mmp-9, and a bp, the collagenase isolated from clostridium histolyticum (chc). the new derivatives generally showed inhibition constants in the range of 8-62 nm against the ... | 2003 | 14506914 |
| enzymes of clostridium welchii type a and clostridium histolyticum that disintegrate decalcified human tooth dentine. | | 1950 | 14778940 |
| the alpha, beta and gamma antigens of clostridium histolyticum (weinberg & séguin, 1916). | | 1950 | 14778941 |
| the serological identification of cultures of clostridium histolyticum as group iii of the proteolytic clostridia. | | 1952 | 14888966 |
| the structure-function relationship in the clostripain family of peptidases. | in this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. a benzamidinyl-diazo dichlorotriazine dye (bdd) was shown to act as an efficient active site-directed affinity label for clostridium histolyticum clostripain. the enzyme, upon incubation with bdd in 0.1 m hepes/naoh buffer ph 7.6, exhibits a time-dependent loss of activity. the ... | 2004 | 15009210 |
| [the influences of lanthanum,cerium and fluorine ions to the activity of collagenase degrading cemental collagens] | the influences of trace elements lanthanum,cerium and fluorine to the activity of clostridium histolyticum collagenase(chc) during the period of chc degrading the cemental root collagen in vitro is studied.the results reveal that la(3+) can significantly inhibit the activity of the chc than ce(3+) and f(-). therefore it suggests that lanthanum may be used as a new kind of element in preventing root caries.the mechanisms of above functions are also discussed. | 1997 | 15159961 |
| inhibition effects of (+)-catechin-aldehyde polycondensates on proteinases causing proteolytic degradation of extracellular matrix. | inhibition effects of (+)-catechin-aldehyde polycondensates against the activity of proteinases, clostridium histolyticum collagenase (chc) and human neutrophil elastase (hne) causing proteolytic degradation of extracellular matrix (ecm), have been investigated. in normal tissues, a balance is reached between the formation and destruction of ecm, leading to a state of homeostasis. however, uncontrolled destruction of ecm contributes to tumor invasion and metastasis. in the measurement of the inh ... | 2004 | 15207729 |
| quantitative analysis of the impact of the organic matrix on the fluoride effect on erosion progression in human dentine using longitudinal microradiography. | after an initial demineralisation, an intensive fluoridation is capable of inhibiting the erosive mineral loss in dentine completely, which might be related to the presence of the exposed organic dentine matrix. aim of the present study was, therefore, to evaluate whether fluoride is also effective when the organic material is removed. the study was a cyclic de- and remineralisation model over 5 days in vitro. samples from human coronal dentine were demineralised with citric acid (ph 2.3; 6 x 10 ... | 2004 | 15353250 |
| interaction of aldehydes with collagen: effect on thermal, enzymatic and conformational stability. | stabilization of type i rat tail tendon (rtt) collagen by various aldehydes, viz. formaldehyde, gluteraldehyde, glyoxal and crotanaldehyde was studied to understand the effect of each on the thermal, enzymatic and conformational stability of collagen. the aldehydes have been found to increase the heat stability of rat tail tendon collagen fibres from 62 to 77-86 degrees c. the increase in thermal stability was found to be in a species dependent manner. the variation in the thermal stability of c ... | 2004 | 15374680 |
| outbreak of clostridium histolyticum infections in injecting drug users in england and scotland. | clostridial infections in injecting drug users in the united kingdom are a relatively new phenomenon that came to light in 2000 when cases of serious illness and deaths due to clostridium novyi were recorded. in the period december 2003 to april 2004, the anaerobe reference laboratory received twelve referrals of an extremely rare isolate, clostridium histolyticum, from cases of infection in injecting drug users submitted from nine different hospitals in england and scotland. molecular typing of ... | 2004 | 15381836 |
| production, purification, and some properties of clostridium histolyticum collagenase. | | 1950 | 15440885 |
| factor xiii contrasts the effects of metalloproteinases in human dermal fibroblast cultured cells. | matrix metalloproteinases (mmps) are overexpressed in venous leg ulcers, determining a breakdown of the main extracellular matrix (ecm) components owing mainly to collagenase activities, and so playing a crucial role in ulcer pathogenesis. the authors studied the effects of coagulation factor xiii (fxiii), which cross-links collagen and other ecm components, in human fibroblast cultured cells in the presence and in the absence of matrix metalloproteinases from clostridium histolyticum collagenas ... | 2004 | 15490040 |
| quantum theoretic qsar of benzene derivatives: some enzyme inhibitors. | our previously developed approach to the development of qsar equations for benzene derivatives, originally for phenylalkylamine hallucinogens, has been applied to four new systems: sulfonamide inhibitors of the enzymes carbonic anhydrase, thrombin, trypsin, and clostridium histolyticum collagenase. the novel features involve the energies and nodal orientations of pi-like orbitals, and an allowance for the symmetry of the benzene nucleus. the resulting equations give better fits, better predictiv ... | 2004 | 15499995 |
| improved enzymatic isolation of fibroblasts for the creation of autologous skin substitutes. | the number of medical applications using autologous fibroblasts is increasing rapidly. we investigated thoroughly the procedure to isolate cells from skin using the enzymatic tissue dissociation procedure. tissue digestion efficiency, cell viability, and yield were investigated in relation to size of tissue fragments, digestion volume to tissue ratio, digestion time, and importance of other protease activities present in clostridium histolyticum collagenase (chc) (neutral protease, clostripain, ... | 2004 | 15723562 |
| an unusual cause of soft tissue infection. | | 2005 | 15951543 |
| modulation of the fecal microbiota by the intake of a lactobacillus johnsonii la1-containing product in human volunteers. | lactobacillus johnsonii la1 (la1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. this study evaluates how the ingestion of different amounts of la1 influences the main bacterial populations of the fecal microbiota. eight asymptomatic volunteers participated in the study. after a basal period, they ingested daily 100 ml of a product containing 10(8) cfu ml(-1) of la1 during the first week, 200 ml during the second week a ... | 2005 | 15970400 |
| a novel galactooligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo. | prebiotics are nondigestible food ingredients that encourage proliferation of selected groups of the colonic microflora, thereby altering the composition toward a more beneficial community. in the present study, the prebiotic potential of a novel galactooligosaccharide (gos) mixture, produced by the activity of galactosyltransferases from bifidobacterium bifidum 41171 on lactose, was assessed in vitro and in a parallel continuous randomized pig trial. in situ fluorescent hybridization with 16s r ... | 2005 | 15987856 |
| [substrate specificity of collagenase of streptomyces sp. 1349 and keratinase of streptomyces sp. 1382]. | the paper determines the action specificity concerning the bonding type of collagenases of strepomyces sp. 1349 and keratinases of streptomyces sp. 1382. experimental data obtained evidence for the wide specificity of the obtained enzymatic drugs. it has been established that both collagenases and keratinases display high specificity in respect of the bonds made by the residues of hydrophobic amino acids. collagenases of streptomyces sp. 1349, as to their wide action specificity, differed from c ... | 2005 | 16018211 |
| differences between the gut microflora of children with autistic spectrum disorders and that of healthy children. | children with autistic spectrum disorders (asds) tend to suffer from severe gastrointestinal problems. such symptoms may be due to a disruption of the indigenous gut flora promoting the overgrowth of potentially pathogenic micro-organisms. the faecal flora of patients with asds was studied and compared with those of two control groups (healthy siblings and unrelated healthy children). faecal bacterial populations were assessed through the use of a culture-independent technique, fluorescence in s ... | 2005 | 16157555 |
| new worlds to explore. | | 2006 | 16439327 |
| proteolysis and the selective destruction of amino acids by clostridium sporogenes and clostridium histolyticum. | | 1929 | 16559359 |
| variability in morphological and biochemical properties of clostridium histolyticum (weinberg and seguin). | | 1937 | 16560062 |
| some serological aspects of the s-r change in clostridium histolyticum. | | 1937 | 16560063 |
| prevalence and temporal stability of selected clostridial groups in irritable bowel syndrome in relation to predominant faecal bacteria. | the differences in faecal bacterial population between irritable bowel syndrome (ibs) and control subjects have been reported in several studies. the aim of the present study was to compare the predominant and clostridial faecal microbiota of ibs subjects and healthy controls by applying denaturing gradient gel electrophoresis (dgge) and a recently developed multiplexed and quantitative hybridization-based technique, transcript analysis with the aid of affinity capture (trac). according to the r ... | 2006 | 16585652 |
| studies on the proteinase of clostridium histolyticum. | | 1937 | 16746452 |
| proteinase secretion and growth of clostridium histolyticum. | | 1938 | 16746798 |
| studies on the endo-enzymes, particularly the peptidases, of clostridium histolyticum. | | 1938 | 16746799 |
| enzymic adaptation in clostridium histolyticum. | | 1938 | 16746800 |
| the proteinases of clostridium histolyticum. | | 1940 | 16747287 |
| molecular characterisation of the gut microflora of healthy and inflammatory bowel disease cats using fluorescence in situ hybridisation with special reference to desulfovibrio spp. | inflammatory bowel disease (ibd) is a common cause of chronic large bowel diarrhoea in cats. although the aetiology of ibd is unknown, an immune-mediated response to a luminal antigen is thought to be involved. as knowledge concerning the colonic microflora of cats is limited and requires further investigation, the purpose of this study was to determine the presence of specific bacterial groups in normal and ibd cats, and the potential role they play in the health of the host. total bacterial po ... | 2007 | 17217390 |
| factors influencing the collagenase digestion phase of human islet isolation. | substantial advances in human islet isolation technology have occurred during the past decade. however, it is still difficult to recover the entire quantity of islets contained in a pancreas. a major obstacle to successful human islet isolation has been the variability of the collagenase digestion phase of islet isolation. future advances in enzyme technology will make it possible to optimally liberate islets with enzyme blends "tailor-made" for each individual donor pancreas. such innovative st ... | 2007 | 17220782 |
| lethal factor of clostridium histolyticum kills cells by apoptosis. | in this study, for the first time, clostridium histolyticum lethal factor was purified by ammonium sulfate precipitation of culture broth, centrifugation through an amicon filter device and hydrophobic interaction chromatography. the purified lethal factor was devoid of proteolytic activity. at a concentration of 150 ng ml(-1) the lethal factor killed 50% of hela cells within 24 h of exposure. abrogation of actin filaments, activation of caspases, fragmentation of nuclear dna as well as ultrastr ... | 2007 | 17328764 |
| differences between the fecal microbiota of coeliac infants and healthy controls. | coeliac disease (cd) is an immune-mediated enteropathy with a multifactorial aetiology, characterized by chronic inflammation of the small intestinal mucosa. although evidence suggests that the gut microbiota contributes to other chronic inflammatory disorders, its possible role in cd has not been determined. in this study, the composition of the fecal microbiota of coeliac children and age-matched controls was investigated by culture-dependent and -independent methodologies, using fluorescent i ... | 2007 | 17489434 |
| imbalance in the composition of the duodenal microbiota of children with coeliac disease. | coeliac disease (cd) is the most common immune-mediated enteropathy characterized by chronic inflammation of the small intestinal mucosa. the ingestion of gluten is responsible for the symptoms of cd, but other environmental factors are also thought to play a role in this disorder. in this study, the composition of the duodenal microbiota of coeliac children with active disease, symptom-free cd patients on a gluten-free diet and control children was determined. bacteriological analyses of duoden ... | 2007 | 18033837 |
| characterization of the gastrointestinal mucosa-associated microbiota of pigs and chickens using culture-based and molecular methodologies. | the microbiota of the gastrointestinal tract (git) can play an important role in the healthy status of farm animals and in the safety of the whole food chain. in this study, the mucosa-associated microbiota of the git of pigs and chickens was analyzed by culture methods and fluorescence in situ hybridization combined with flow cytometry (fcm-fish). in all pig git sections, lactic acid bacteria, enterobacteriaceae, atopobium, bacteroides, and clostridium histolyticum were the predominant bacteria ... | 2007 | 18095433 |
| in vitro fermentation of oat and barley derived beta-glucans by human faecal microbiota. | fermentation of beta-glucan fractions from barley [average molecular mass (mm), of 243, 172, and 137 kda] and oats (average mm of 230 and 150 kda) by the human faecal microbiota was investigated. fractions were supplemented to ph-controlled anaerobic batch culture fermenters inoculated with human faecal samples from three donors, in triplicate, for each substrate. microbiota changes were monitored by fluorescent in situ hybridization; groups enumerated were: bifidobacterium genus, bacteroides an ... | 2008 | 18430007 |
| molecular analysis of the digestive microbiota in a gnotobiotic mouse model during antibiotic treatment: influence of saccharomyces boulardii. | the probiotic saccharomyces boulardii is a non-pathogenic yeast that has been proven efficient in the prevention of antimicrobial-associated diarrhea and of clostridium difficile associated colitis. we evaluated the influence of the administration of s. boulardii on the composition of the fecal microbiota in a human microbiota-associated mouse model. this evaluation was run before, during and after a 7-day oral treatment with amoxicillin clavulanic acid. predominant groups of bacteria were quant ... | 2008 | 18511310 |
| broad-range pcr as a supplement to culture for detection of bacterial pathogens in patients with a clinically diagnosed spinal infection. | we aimed to evaluate broad-range pcr and subsequent sequencing compared to conventional culture in the diagnosis of spinal infection. the method was a prospective study of all patients admitted to aarhus university hospital for surgery during a 12-months period with a clinically diagnosed infection of the spine. samples from patients undergoing surgery for non-infectious causes (malignancy etc.) were included as control group. specimens were submitted to conventional culture and molecular invest ... | 2008 | 18609207 |
| the activation mechanism and physicochemical properties of clostridium histolyticum proteinase. | | 1948 | 18871209 |
| [molecular diagnosis of osteogenesis imperfecta type i]. | osteogenesis imperfecta (oi) is caused by mutations in collagen type i genes. in contrast to oi type ii, iii and iv where there are the structural mutations, in oi type i decreased production of normal collagen is due to the presence of a null allele. because both pharmacological and gene therapy approaches depend on type of mutation and its consequences, quick and proper diagnosis is required. | 2008 | 19145934 |
| pfizer swallows wyeth, validates niche buster. | | 2009 | 19270661 |
| purification and characterization of an eggshell membrane decomposing protease from pseudomonas aeruginosa strain me-4. | a bacterial strain, me-4, isolated from farm soil and identified as pseudomonas aeruginosa, grew well on a medium containing eggshell membrane (esm). p. aeruginosa strain me-4 decomposed the esm by producing an extracellular protease able to solubilize it. the protease was purified to homogeneity from culture supernatant by fractionation with (nh(4))(2)so(4), as well as cm52 cellulose and de52 cellulose column chromatography, with a final yield of 47%. the molecular mass of the enzyme was 33 kda ... | 2009 | 19332295 |
| qsar analysis of some 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases and bacterial collagenase. | a quantitative structure-activity relationship (qsar) study has been performed on 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases (mmps) and a bacterial collagenase known as clostridium histolyticum collagenase (chc) to understand the structural features influencing the affinity of these inhibitors towards the enzyme. the compounds in the selected series were characterized by topological and fragmental descriptors calculated using quasar module of molecular ope ... | 2006 | 16682189 |
| vacuolization of hela cells by a partially purified clostridium histolyticum cytotoxin. | clostridium histolyticum vacuolating cytotoxin was partially purified from culture broth using ammonium sulfate precipitation, gel filtration and hydrophobic interaction chromatography. the toxin caused vacuolization of hela cells visible under a light microscope after 2 h and distinct after 8 h. transmission electron microscopy revealed the presence of numerous vacuoles, condensation of the mitochondrial matrix, increased cytoplasm density and increased amounts of heterochromatin. apoptosis was ... | 2006 | 16553808 |
| the in vitro effect of a collagenolytic enzyme inhibitor on lesion development in root dentin. | to evaluate the effects of a new active collagenase inhibitor, pirocton olamine (po), on acid demineralization in dentin and to investigate possible mechanisms of the inhibitory effects. | 2009 | 19626976 |
| 1h, 13c and 15n resonance assignments of ca2+ bound collagen-binding domain derived from a clostridial collagenase. | (1)h, (15)n, and (13)c nmr assignments for 116 amino acids (gly893-lys1008) of a bacterial collagen-binding domain (cbd) derived from clostridium histolyticum class i collagenase were accomplished. clostridial collagenases hydrolyze insoluble collagen. one to three copies of collagen-binding domains (cbds) are present at their c-termini, each of which is the minimal segment required for the binding to the insoluble substrate. cbd has been shown to be able to anchor fused growth factors for up to ... | 2008 | 19636886 |
| effect of a milk formula with prebiotics on the intestinal microbiota of infants after an antibiotic treatment. | antibiotics exert deleterious effects on the intestinal microbiota, favoring the emergence of opportunistic bacteria and diarrhea. prebiotics are nondigestible food components that stimulate the growth of bifidobacteria. our aim was to evaluate the effects on the intestinal microbiota of a prebiotic-supplemented milk formula after an antibiotic treatment. a randomized, double-blind, controlled clinical trial was carried out in 140 infants 1-2 y of age distributed into two groups after a 1-wk amo ... | 2006 | 16492988 |
| injectable collagenase clostridium histolyticum for dupuytren's contracture. | dupuytren's disease limits hand function, diminishes the quality of life, and may ultimately disable the hand. surgery followed by hand therapy is standard treatment, but it is associated with serious potential complications. injection of collagenase clostridium histolyticum, an office-based, nonsurgical option, may reduce joint contractures caused by dupuytren's disease. | 2009 | 19726771 |
| inhibition of collagenase by naturally-occurring flavonoids. | we examined the inhibitory activities of various flavonoids, including the flavanones, flavones/isoflavones and flavonols, on collagenase from clostridium histolyticum to establish their therapeutic potential against skin inflammation and photoaging. in general, the flavonols were stronger inhibitors than the flavones/isoflavones, and this indicated the importance of the c-3 hydroxyl substitution. quercetin was the most active flavonoid among those tested, and it showed an ic50 of 286 microm. th ... | 2005 | 16276971 |
| determination of the cytotoxic effect of clostridium histolyticum culture supernatant on hela cells in the presence of protease inhibitors. | clostridium histolyticum culture supernatant contains numerous enzymes, which exert a cytotoxic effect on host cells. this includes lethal toxin, clostripain and high-potassium-sensitive toxin. since the number of c. histolyticum infections increased during the last several years, it seems worthwhile to evaluate whether protease inhibitors, used for the treatment of many diseases, could influence toxicity, and thus, pathogenicity of c. histolyticum. in this study we evaluated in vitro the influe ... | 2005 | 16051065 |
| [a mass-spectrometric approach to primary screening of collagenolytic enzymes]. | a comparative analysis of maldi tof mass spectra of low-molecular products resulting from the hydrolysis of native collagen i by collagenases of various classes (bacterial metallocollagenase from clostridium histolyticum, serine collagenase from the morikrasa commercial preparation, cysteine collagenase from serratia proteomaculans, and cysteine collagenases from larvae of beetles dermestesfrischi and d. maculates) was carried out. the spectra contain a number of ion peaks common for all collage ... | 2008 | 18672690 |
| crystallization and preliminary x-ray characterization of the catalytic domain of collagenase g from clostridium histolyticum. | the catalytic domain of collagenase g from clostridium histolyticum has been cloned, recombinantly expressed in escherichia coli and purified using affinity and size-exclusion column-chromatographic methods. crystals of the catalytic domain were obtained from 0.12 m sodium citrate and 23%(v/v) peg 3350 at 293 k. the crystals diffracted to 2.75 a resolution using synchrotron radiation. the crystals belong to an orthorhombic space group, with unit-cell parameters a = 57, b = 109, c = 181 a. this u ... | 2008 | 18453715 |
| an internally quenched fluorescent substrate for collagenase. | a synthetic collagenase substrate containing the internal peptide sequence--gly-gly-pro-leu-gly-pro-pro-gly-pro--has been synthesized, with an n-terminus 4-((4-(dimethylamino)phenyl)azo)-benzoyl (dabcyl) group and c-terminus 5-[2-(acetamido)ethylamino] naphthalene-1-sulfonic acid (aedans) moiety resulting in internal quenching of aedans fluorescence. peptide bond hydrolysis results in a large increase in fluorescence at 490 nm upon excitation at 336 nm. the substrate is cleaved exclusively by cl ... | 2008 | 18260138 |
| effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat. | effects of collagen digestion have been defined up to the fibril level. however, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ecm) affects a muscle's passive elastic response. various elastography methods have been applied as tools for evaluating the mechanical properties and ecm content of skeletal muscle. in an effort to develop an ecm altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic prope ... | 2010 | 19945332 |
| injectable collagenase clostridium histolyticum for dupuytren's contracture. | | 2009 | 20032329 |
| clostridium histolyticum infection. | | 1949 | 18146655 |
| structure of the claudin-binding domain of clostridium perfringens enterotoxin. | clostridium perfringens enterotoxin is a common cause of food-borne and antibiotic-associated diarrhea. the toxin's receptors on intestinal epithelial cells include claudin-3 and -4, members of a large family of tight junction proteins. toxin-induced cytolytic pore formation requires residues in the nh(2)-terminal half, whereas residues near the cooh terminus are required for binding to claudins. the claudin-binding cooh-terminal domain is not toxic and is currently under investigation as a pote ... | 2008 | 17977833 |
| effect of a new synbiotic mixture on atopic dermatitis in infants: a randomized-controlled trial. | clinical trials investigating the therapeutic effect of probiotics on atopic dermatitis (ad) show inconsistent results. better results can possibly be achieved by combining probiotics with prebiotics, i.e. synbiotics. | 2010 | 20184604 |
| successful human islet isolation and transplantation indicating the importance of class 1 collagenase and collagen degradation activity assay. | purified tissue dissociation enzymes (tdes) are critical to successful human islet isolation required for clinical transplantation, but little is known about the characteristics of the key enzymes-class i (c1) and class ii (c2) collagenase from clostridium histolyticum-used in these procedures. here, we show the differences between the c1 collagenase found in purified collagenase products manufactured by three suppliers and the impact of differences in c1 between two suppliers on human islet yie ... | 2010 | 20300051 |
| the collagenase activity of culture filtrates of clostridium histolyticum. | | 1947 | 20344283 |
| in vitro binding of matrix metalloproteinase-2 (mmp-2), mmp-9, and bacterial collagenase on collagenous wound dressings. | chronic wounds are characterized by failure in wound-healing response and a delay in healing or nonclosure of the wounds. this results in a high effort in clinical treatment and/or home care. a major difference between acute wounds and chronic wounds is the imbalance of proteinase inhibitors and proteinase activity that regulates the degradation and regeneration of the extracellular matrix proteins. collagen and collagen/oxidized regenerated cellulose dressings act as a competitive substrate for ... | 2007 | 17650099 |
| new treatment options for dupuytren's surgery: collagenase and percutaneous aponeurotomy. | | 2010 | 20434276 |
| antiadhesive effects of mitomycin c and streptopeptidase a in rats with intraperitoneal adhesions. | intraabdominal adhesion formation is a frequent problem after major abdominal surgery. for many years, there have been various attempts to decrease adhesions by using systemic and local drugs and mechanical barriers. in this study we aimed to evaluate the antifibrinolytic antiadhesive effects of mitomycin c (mmc) and streptopeptidase a (sa) against intraabdominal adhesions. forty-eight rats were divided into six groups, each with eight rats. group 1 (sham group) rats were laparotomized by transv ... | 2007 | 17579872 |