| antimicrobial effects of ozonated water on the sanitization of dental instruments contaminated with e. coli, s. aureus, c. albicans, or the spores of b. atrophaeus. | ozone has been used as an alternative method for the decontamination of water, food, equipment and instruments. the objective of this study was to evaluate the antimicrobial effects of ozonated water on the sanitization of dental instruments that were contaminated by escherichia coli, staphylococcus aureus, candida albicans and the spores of bacillus atrophaeus. | 2012 | 23021648 |
| comparative analysis of classical and molecular microbiology methods for the detection of escherichia coli and enterococcus spp. in well water. | the microbiological quality of 165 1 litre well water samples collected in the québec city region was assessed by culture-based methods (mfc agar, chromocult coliform agar, colilert(®), mi agar, chromocult enterococci, enterolert™, and mei agar) and by a molecular microbiology strategy, dubbed crename-rtpcr, developed for the detection of escherichia coli, enterococcus spp., enterococcus faecalis/faecium, and bacillus atrophaeus subsp. globigii. in these drinking water samples, approved culture- ... | 2012 | 23014932 |
| blue light (470 nm) effectively inhibits bacterial and fungal growth. | blue light (470 nm) led antimicrobial properties were studied alone against bacteria and with or without the food grade photosensitizer, erythrosine (ery) against filamentous fungi. leuconostoc mesenteroides (lm), bacillus atrophaeus (ba) or pseudomonas aeruginosa (pa) aliquots were exposed on nutrient agar plates to array 1 (ar1, 0·2 mw cm(-2)) or array 2 (ar2, 80 mw cm(-2)), which emitted impure or pure blue light (0-300 j cm(-2)), respectively. inoculated control (room light only) plates were ... | 2012 | 23009190 |
| glycerol-based sterilization bioindicator system from bacillus atrophaeus: development, performance evaluation, and cost analysis. | the development of new value-added applications for glycerol is of worldwide interest because of the environmental and economic problems that may be caused by an excess of glycerol generated from biodiesel production. a novel use of glycerol as a major substrate for production of a low-cost sterilization biological indicator system (bis; spores on a carrier plus a recovery medium) was investigated. a sequential experimental design strategy was applied for product development and optimization. th ... | 2013 | 22911095 |
| draft genome sequence of the sponge-associated strain bacillus atrophaeus c89, a potential producer of marine drugs. | bacillus atrophaeus c89, isolated from the marine sponge dysidea avara, is a potential producer of bioactive compounds, such as neobacillamide a and bacillamide c. here, we present a 4.2-mb assembly of its genome. the nonribosomal peptide synthetases (nrpss) make it possible to produce the bioactive compounds. | 2012 | 22843588 |
| passive detection of biological aerosols in the atmosphere with a fourier transform instrument (ftir)--the results of the measurements in the laboratory and in the field. | fourier transform infrared radiation (ftir) spectroscopy is one of the most powerful methods for the detection of gaseous constituents, aerosols, and dust in planetary atmospheres. infrared spectroscopy plays an important role in searching for biomarkers, organics and biological substances in the universe. the possibility of detection and identifications with ftir spectrometer of bio-aerosol spores (bacillus atrophaeus var. globigii=bg) in the atmosphere is discussed in this paper. we describe t ... | 2012 | 22707349 |
| inactivation of aerosolized bacillus atrophaeus (bg) endospores and ms2 viruses by combustion of reactive materials. | accidental release of biological agents from a bioweapon facility may contaminate large areas, possibly causing disastrous environmental consequences. to address this issue, novel halogen-containing reactive materials are being designed with the added capability to inactivate viable airborne microorganisms. this study determined the efficiency of combustion products of such materials to inactivate aerosolized bacteria and viruses. spores of bacillus atrophaeus and ms2 viruses dispersed in dry ai ... | 2012 | 22662743 |
| relations between phenotypic changes of spores and biofilm production by bacillus atrophaeus atcc 9372 growing in solid-state fermentation. | bacillus spp. spores are usually obtained from strains cultivated in artificial media. however, in natural habitats, spores are predominantly formed from bacteria present in highly surface-associated communities of cells. solid-state fermentation (ssf) is the culture method that best mimetizes the natural environment of many microorganisms that grow attached to the surface of solid particles. this study aims to confirm that sporulation through ssf of bacillus atrophaeus occurs by biofilm formati ... | 2012 | 22526268 |
| low-temperature decontamination with hydrogen peroxide or chlorine dioxide for space applications. | the currently used microbial decontamination method for spacecraft and components uses dry-heat microbial reduction at temperatures of >110°c for extended periods to prevent the contamination of extraplanetary destinations. this process is effective and reproducible, but it is also long and costly and precludes the use of heat-labile materials. the need for an alternative to dry-heat microbial reduction has been identified by space agencies. investigations assessing the biological efficacy of tw ... | 2012 | 22492450 |
| bacillus atrophaeus inactivated spores as a potential adjuvant for veterinary rabies vaccine. | rabies is a viral encephalitis, nearly always fatal, but preventable through vaccines. rabid animal bite is the prime transmission act, while veterinary vaccination is one of the best strategies for rabies general prevention. aluminum compounds and saponin are the commercial adjuvants used for this vaccine nowadays. nevertheless, aluminum compounds can provoke undesired side effects and saponin has a narrow activity range without toxicity. b. atrophaeus inactivated spores (bais), with or without ... | 2012 | 22449426 |
| fast and effective: intense pulse light photodynamic inactivation of bacteria. | the goal of this study was to investigate the photodynamic toxicity of tmpyp (5, 10, 15, 20-tetrakis (1-methylpyridinium-4-yl)-porphyrin tetra p-toluenesulfonate) in combination with short pulses (ms) of an intense pulse light source within 10 s against bacillus atrophaeus, staphylococcus aureus, methicillin-resistant s. aureus and escherichia coli, major pathogens in food industry and in health care, respectively. bacteria were incubated with a photoactive dye (tmpyp) that is subsequently irrad ... | 2012 | 22354734 |
| laboratory evaluation of large-scale decontamination approaches. | to evaluate the effectiveness of two spray-based decontamination methods for surface contamination reduction and to determine the potential for contamination spread by these methods. | 2012 | 22332972 |
| a model of the transmission of micro-organisms in a public setting and its correlation to pathogen infection risks. | gastro-intestinal infections are widespread in the community and have considerable economic consequences. in this study, we followed chains of infection from a public toilet scenario, looking at infection risks by correlating the transmission of bacteria, fungi and viruses to our current knowledge of infectious doses. | 2012 | 22229855 |
| evaluation of the sterilization efficacy of domestic electric drills used in orthopaedic surgeries. | it is estimated that electric drills (ed) have been used in orthopaedic surgeries for bone drilling for more than 50 years in brazilian hospitals. it is an electric, thermosensitive equipment, not indicated for surgical use, which has not been previously evaluated regarding the sterilization efficacy, being suspect of infection risk. this study evaluated the efficacy of sterilization by ethylene oxide (eto) of new drills that were intentionally contaminated with bacillus atrophaeus spores. an ex ... | 2009 | 24031397 |
| comparative analysis of the sensitivity of metagenomic sequencing and pcr to detect a biowarfare simulant (bacillus atrophaeus) in soil samples. | to evaluate the sensitivity of high-throughput dna sequencing for monitoring biowarfare agents in the environment, we analysed soil samples inoculated with different amounts of bacillus atrophaeus, a surrogate organism for bacillus anthracis. the soil samples considered were a poorly carbonated soil of the silty sand class, and a highly carbonated soil of the silt class. control soil samples and soil samples inoculated with 10, 103, or 105 cfu were processed for dna extraction. about 1% of the d ... | 2017 | 28472119 |
| is the stella™ 5l system an effective cold sterilization technique for needle-based confocal miniprobes? | needle-based confocal laser endomicroscopy (ncle) under endoscopic ultrasound guidance is a newly developed imaging technique for pancreatic lesions and lymph nodes, enabling a subcellular level of resolution. the confocal miniprobe is an invasive instrument designed to be reused up to 10 times. therefore, a method that ensures the complete elimination of microbial contaminants on the device is necessary. we studied the bactericidal efficacy of the stella™ system, which purports to achieve this ... | 2017 | 28621298 |
| squamocin, an annonaceous acetogenin, enhances naphthalene degradation mediated by bacillus atrophaeus cn4. | squamocin belongs to a group of compounds called annonaceous acetogenins. they are secondary products of annonaceae metabolism and can be isolated from annona cherimolia seeds. this paper deals with the stimulation of biofilm formation of bacillus atrophaeus cn4 by employing low squamocin concentrations to increase naphthalene degradation. bacillus atrophaeus cn4, isolated from contaminated soil, has the ability to degrade naphthalene as the only source of carbon and energy. in the absence of ad ... | 2017 | 28554707 |
| effect of bacteria type and sucrose concentration on levan yield and its molecular weight. | levan has been traditionally produced from microorganism. however, there is a continuous effort in looking for new strains that improve levan production yield and uses alternative sugar sources for growth. despite having a wide range of data about levan yield, there are not papers which allow controlling molecular weight, and that plays an essential role for further applications. | 2017 | 28535808 |