| thermal inactivation of bacillus anthracis surrogate spores in a bench-scale enclosed landfill gas flare. | a bench-scale landfill flare system was designed and built to test the potential for landfilled biological spores that migrate from the waste into the landfill gas to pass through the flare and exit into the environment as viable. the residence times and temperatures of the flare were characterized and compared to full-scale systems. geobacillus stearothermophilus and bacillus atrophaeus, nonpathogenic spores that may serve as surrogates for bacillus anthracis, the causative agent for anthrax, w ... | 2012 | 22442931 |
| heterologous expression of γ-glutamyl transpeptidase from bacillus atrophaeus gs-16 and its application in the synthesis of γ-d-glutamyl-l-tryptophan, a known immunomodulatory peptide. | gamma-glutamyl transpeptidase from a mesophilic bacterium bacillus atrophaeus gs-16 (baggt) was expressed heterologously in e. coli using pet-51b vector. maximum production of baggt was obtained at 16°c after 16h of iptg induction and the protein, in its native conformation, was active as a heterooctamer which was composed of four heterodimeric units combined together. one heterodimeric unit constituted two subunits with molecular masses of 45kda and 21kda, respectively. the recombinant enzyme w ... | 2017 | 28193333 |
| highly sensitive sequence specific qpcr detection of mycobacterium tuberculosis complex in respiratory specimens. | nucleic acid amplification tests for mycobacterium tuberculosis (mtb) detection from sputum are highly sensitive and specific with smear microscopy positive specimens, but their sensitivity with smear-negative/culture-positive specimens is much lower; therefore, these tests cannot rule out a tuberculosis diagnosis. co-extraction of pcr inhibitors may be a cause of decreased test sensitivity. here the design and early validation of a mtb screening assay with sample preparation and qpcr methods de ... | 2016 | 27865380 |
| effect of static magnetic field on endospore germination. | this work investigated the effect of static magnetic field (smf) on bacillus atrophaeus endospore germination. germination was triggered by l-alanine in 1.3-t smf and characterized by ion release, ca(2+) -dipicolinic acid release, and water influx. these events were monitored by electrical conductivity, tb-dpa fluorescence, and optical density, respectively. culturability of endospore germinated in smf exposure was evaluated by cfu enumeration. results indicated that 1.3-t smf failed to signific ... | 2017 | 27862061 |
| production of lipopeptide biosurfactants by bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery. | lipopeptides are known as promising microbial surfactants and have been successfully used in enhancing oil recovery in extreme environmental conditions. a biosurfactant-producing strain, bacillus atrophaeus 5-2a, was recently isolated from an oil-contaminated soil in the ansai oilfield, northwest china. in this study, we evaluated the crude oil removal efficiency of lipopeptide biosurfactants produced by b. atrophaeus 5-2a and their feasibility for use in microbial enhanced oil recovery. | 2016 | 27716284 |
| overproduction and characterization of a lytic polysaccharide monooxygenase in bacillus subtilis using an assay based on ascorbate consumption. | lytic polysaccharide monooxygenases (lpmos) are copper ion-containing enzymes that degrade crystalline polysaccharides, such as cellulose or chitin, through an oxidative mechanism. to the best of our knowledge, there are no assay methods for the direct characterization of lpmos that degrade substrates without coupled enzymes. as such, in this study, a coupled enzyme-free assay method for lpmos was developed, which is based on measuring the consumption of ascorbic acid used as an external electro ... | 2016 | 27702475 |
| surfactin variants mediate species-specific biofilm formation and root colonization in bacillus. | cyclic lipopeptides (clp) and especially surfactins produced by bacillus spp. trigger biofilm formation and root colonization and are crucial for biocontrol activity and systemic resistance in plants. bacillus atrophaeus 176s isolated from the moss tortella tortuosa produces the clp fengycins, iturins and surfactins, possesses antifungal activities and can protect tomato, lettuce and sugar beet against rhizoctonia solani infection. in b. atrophaeus we identified for the first time the variant su ... | 2016 | 27306252 |
| peracetic acid: a practical agent for sterilizing heat-labile polymeric tissue-engineering scaffolds. | advanced biomaterials and sophisticated processing technologies aim at fabricating tissue-engineering scaffolds that can predictably interact within a biological environment at the cellular level. sterilization of such scaffolds is at the core of patient safety and is an important regulatory issue that needs to be addressed before clinical translation. in addition, it is crucial that meticulously engineered micro- and nano- structures are preserved after sterilization. conventional sterilization ... | 2014 | 24341350 |
| lipopeptides, a novel protein, and volatile compounds contribute to the antifungal activity of the biocontrol agent bacillus atrophaeus cab-1. | bacillus atrophaeus cab-1 displays a high inhibitory activity against various fungal pathogens and suppresses cucumber powdery mildew and tomato gray mold. we extracted and identified lipopeptides and secreted proteins and volatile compounds produced by strain cab-1 to investigate the mechanisms involved in its biocontrol performance. in vitro assays indicated all three types of products contributed to the antagonistic activity against the fungal pathogen botrytis cinerea. each of these componen ... | 2013 | 24013222 |
| uncertainty analysis of the recovery of hollow-fiber ultrafiltration for multiple microbe classes from water: a bayesian approach. | in this study, we introduce a bayesian approach to address uncertainty of microbial recoveries from hollow-fiber ultrafilters (hfuf) and to determine any sources of uncertainty. microbial recoveries were measured under twenty conditions, including two types of water, two types of ultrafilters, and five types of microorganisms. the probability distributions of the recoveries were approximated using bayesian statistics with markov chain monte carlo sampling after integrating the likelihood functio ... | 2013 | 23524155 |
| evaluation of surface sampling for bacillus spores using commercially available cleaning robots. | five commercially available domestic cleaning robots were evaluated on their effectiveness for sampling aerosol-deposited bacillus atrophaeus spores on different indoor material surfaces. the five robots tested include three vacuum types (r1, r2, and r3), one wet wipe (r4), and one wet vacuum (r5). tests were conducted on two different surface types (carpet and laminate) with 10(6) colony forming units of b. atrophaeus spores deposited per coupon (35.5 cm × 35.5 cm). spores were deposited on the ... | 2013 | 23431954 |
| passive detection of biological aerosols in the atmosphere with a fourier transform instrument (ftir)--the results of the measurements in the laboratory and in the field. | fourier transform infrared radiation (ftir) spectroscopy is one of the most powerful methods for the detection of gaseous constituents, aerosols, and dust in planetary atmospheres. infrared spectroscopy plays an important role in searching for biomarkers, organics and biological substances in the universe. the possibility of detection and identifications with ftir spectrometer of bio-aerosol spores (bacillus atrophaeus var. globigii=bg) in the atmosphere is discussed in this paper. we describe t ... | 2012 | 22707349 |
| inactivation of aerosolized bacillus atrophaeus (bg) endospores and ms2 viruses by combustion of reactive materials. | accidental release of biological agents from a bioweapon facility may contaminate large areas, possibly causing disastrous environmental consequences. to address this issue, novel halogen-containing reactive materials are being designed with the added capability to inactivate viable airborne microorganisms. this study determined the efficiency of combustion products of such materials to inactivate aerosolized bacteria and viruses. spores of bacillus atrophaeus and ms2 viruses dispersed in dry ai ... | 2012 | 22662743 |
| evaluation of the sterilization efficacy of domestic electric drills used in orthopaedic surgeries. | it is estimated that electric drills (ed) have been used in orthopaedic surgeries for bone drilling for more than 50 years in brazilian hospitals. it is an electric, thermosensitive equipment, not indicated for surgical use, which has not been previously evaluated regarding the sterilization efficacy, being suspect of infection risk. this study evaluated the efficacy of sterilization by ethylene oxide (eto) of new drills that were intentionally contaminated with bacillus atrophaeus spores. an ex ... | 2009 | 24031397 |
| comparative analysis of the sensitivity of metagenomic sequencing and pcr to detect a biowarfare simulant (bacillus atrophaeus) in soil samples. | to evaluate the sensitivity of high-throughput dna sequencing for monitoring biowarfare agents in the environment, we analysed soil samples inoculated with different amounts of bacillus atrophaeus, a surrogate organism for bacillus anthracis. the soil samples considered were a poorly carbonated soil of the silty sand class, and a highly carbonated soil of the silt class. control soil samples and soil samples inoculated with 10, 103, or 105 cfu were processed for dna extraction. about 1% of the d ... | 2017 | 28472119 |
| from cellular lysis to microarray detection, an integrated thermoplastic elastomer (tpe) point of care lab on a disc. | we present an all-thermoplastic integrated sample-to-answer centrifugal microfluidic lab-on-disc system (lod) for nucleic acid analysis. the proposed cd system and engineered platform were employed for analysis of bacillus atrophaeus subsp. globigii spores. the complete assay comprised cellular lysis, polymerase chain reaction (pcr) amplification, amplicon digestion, and microarray hybridization on a plastic support. the fluidic robustness and operating efficiency of the assay were ensured throu ... | 2015 | 25385141 |
| squamocin, an annonaceous acetogenin, enhances naphthalene degradation mediated by bacillus atrophaeus cn4. | squamocin belongs to a group of compounds called annonaceous acetogenins. they are secondary products of annonaceae metabolism and can be isolated from annona cherimolia seeds. this paper deals with the stimulation of biofilm formation of bacillus atrophaeus cn4 by employing low squamocin concentrations to increase naphthalene degradation. bacillus atrophaeus cn4, isolated from contaminated soil, has the ability to degrade naphthalene as the only source of carbon and energy. in the absence of ad ... | 2017 | 28554707 |
| effect of bacteria type and sucrose concentration on levan yield and its molecular weight. | levan has been traditionally produced from microorganism. however, there is a continuous effort in looking for new strains that improve levan production yield and uses alternative sugar sources for growth. despite having a wide range of data about levan yield, there are not papers which allow controlling molecular weight, and that plays an essential role for further applications. | 2017 | 28535808 |