| survey of bacterial populations present in us-produced linerboard with high recycle content. | to survey paperboard products from 17 us mills for bacterial populations and for bacteria potentially harmful to human health. | 2010 | 19645765 |
| isolation and characterization of site-specific dna-methyltransferases from bacillus coagulans k. | two site-specific dna methyltransferases, m.bcokia and m.bcokib, were isolated from the thermophilic strain bacillus coagulans k. each of the methylases protects the recognition site 5'-ctcttc-3'/5'-gaagag-3' from cleavage with the cognate restriction endonuclease bcoki. it is shown that m.bcokib is an n6-adenine specific methylase and m.bcokia is an n4-cytosine specific methylase. according to bisulfite mapping, m.bcokia methylates the first cytosine in the sequence 5'-ctcttc-3'. | 2004 | 15061697 |
| antibacterial effects of knotwood extractives on paper mill bacteria. | hydrophilic knotwood extracts from 18 wood species were assessed in disc diffusion and liquid culture tests for antibacterial effects against three species of paper mill bacteria. the pinus sylvestris, p. resinosa, p. contorta, and p. banksiana extracts decreased or inhibited bacterial growth. the susceptibility order was p. sylvestris > p. resinosa > p. contorta > p. banksiana, correlating with the concentrations of pinosylvin and pinosylvin monomethyl ether in these wood species. also, pseudot ... | 2004 | 15112061 |
| rapid degradation of ferulic acid via 4-vinylguaiacol and vanillin by a newly isolated strain of bacillus coagulans. | a new strain bacillus coagulans bk07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. this strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. vanillic acid was further demethylated to protocatechuic acid. above 95% substrate degradation was obtained within 7 h of growth on ferulic acid medium, which is the shortest period of time reported to dat ... | 2000 | 10949310 |
| sau42i, a bcgi-like restriction-modification system encoded by the staphylococcus aureus quadruple-converting phage phi42. | the serotype f phage phi42 of staphylococcus aureus is a triple-converting bacteriophage that encodes the staphylokinase gene (sak) and the enterotoxin a gene (enta). lysogeny results in loss of expression of the chromosomal beta-haemolysin gene (hlb) (negative conversion), the expression of staphylokinase and enterotoxin a (positive conversion), and the acquisition of resistance to lysis by all 23 phages of the international basic set (ibs) of s. aureus typing phages. until this study, the basi ... | 2005 | 15817797 |
| formation of the spore clumps during heat treatment increases the heat resistance of bacterial spores. | effects of the clumping of bacterial spores on their heat resistance as a result of heat treatment were investigated. spore suspensions of bacillus cereus, bacillus coagulans and bacillus licheniformis were heated at 85 degrees c. survivor curves of the three strains showed tailing in all treatments after 30 min. as the treatment time increased, the formation of spore clumps increased in all strains after 20 min. relative hydrophobicity of the spore surface increased as a result of heat treatmen ... | 2005 | 15925006 |
| expression of a thermostable xylanase gene from bacillus coagulans st-6 in lactococcus lactis. | the aim of the study is to evaluate whether xylanase can be used as a potential reporter gene for cloning and expression studies in lactococcus. | 2006 | 16478506 |
| semi-continuous l-lactate fermentation of garbage without sterile condition and analysis of the microbial structure. | in order to develop a simple l-lactate fermentation of organic wastes, acidogenic fermentation was investigated in semi-continuous culture at 55 degrees c with using unsterilized artificial garbage as feedstock. and, sludge from a thermophilic acidification reactor was inoculated at the start of the fermentation. the effects of ph and hydraulic retention time (hrt) on performance of l-lactate production were discussed in a series of operational conditions with regard to long-term stability. the ... | 2007 | 17320925 |
| anion effects on anti-microbial activity of poly[1-vinyl-3-(2-sulfoethyl imidazolium betaine)]. | recent investigations in the anti-microbial properties of the functional polymers are predominantly focused on the structure of the cationic moieties. in the present study, we investigated that the nature of the anion present in polysulfobetaines affects activity against certain microorganisms and their anti-microbial properties have been rationalized in terms of the structure-activity relationship. vinyl imidazolium-based polysulfobetaines were prepared by the quaternization of poly(n-vinyl imi ... | 2010 | 20060120 |
| occurrence of a highly heat-sensitive spore subpopulation of bacillus coagulans stcc 4522 and its conversion to a more heat-stable form. | the profile of the survival curves, at different heating temperatures, of b. coagulans stcc 4522 sporulated at 52(deg)c has been studied, focusing on the early moments of treatment. a highly heat-sensitive spore subpopulation that includes more than 90% of the total spore population has been found. this heat-sensitive spore fraction was inactivated after 2 s of treatment at 111(deg)c. its heat resistance was as much as 200-fold lower than that of the heat-resistant spore fraction (d(inf111(deg)c ... | 1997 | 16535625 |
| crystallization and preliminary x-ray analysis of 3-isopropylmalate dehydrogenase from the moderate facultative thermophile bacillus coagulans. | three crystalline forms of 3-isopropylmalate dehydrogenase from the moderate facultative thermophile bacillus coagulans were obtained by hanging-drop vapor-diffusion methods. one of them, which had crystallized under slightly milder conditions than the others, was suitable for x-ray analysis. its asymmetric unit contains one dimeric molecule and the solvent content is higher than in other protein crystals. the crystal structure was solved in a preliminary manner by the molecular-replacement tech ... | 1996 | 15299617 |
| microbial hydrogen production with bacillus coagulans iit-bt s1 isolated from anaerobic sewage sludge. | bacillus coagulans strain iit-bt s1 isolated from anaerobically digested activated sewage sludge was investigated for its ability to produce h(2) from glucose-based medium under the influence of different environmental parameters. at mid-exponential phase of cell growth, h(2) production initiated and reached maximum production rate in the stationary phase. the maximal h(2) yield (2.28 mol h(2)/molglucose) was recorded at an initial glucose concentration of 2% (w/v), ph 6.5, temperature 37 degree ... | 2007 | 16797976 |
| lactic acid production from lime-treated wheat straw by bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate. | conventional processes for lignocellulose-to-organic acid conversion requires pretreatment, enzymatic hydrolysis, and microbial fermentation. in this study, lime-treated wheat straw was hydrolyzed and fermented simultaneously to lactic acid by an enzyme preparation and bacillus coagulans dsm 2314. decrease in ph because of lactic acid formation was partially adjusted by automatic addition of the alkaline substrate. after 55 h of incubation, the polymeric glucan, xylan, and arabinan present in th ... | 2008 | 18247027 |
| comparison of thermophilic anaerobic digestion characteristics between single-phase and two-phase systems for kitchen garbage treatment. | lab-scale single-phase and two-phase thermophilic methane fermentation systems (sps and tps, respectively) were operated and fed with artificial kitchen waste. in both sps and tps, the highest methane recovery ratio of 90%, in terms of chemical oxygen demand by dichromate (codcr), was observed at an organic loading rate (olr) of 15 gcodcr/(l.d). the ratio of particle codcr remaining to total codcr in the influent was 0.1 and the ratio of nh(4)-n concentration to the input total nitrogen concentr ... | 2008 | 18295719 |
| probiotics for prevention of antibiotic-associated diarrhea. | antibiotic-associated diarrhea (aad) occurs in approximately 25% of patients receiving antibiotics. hospitalized patients with aad are at increased risk for nosocomial infections and have a higher mortality. probiotics are living microorganisms used to restore gut health by changing the intestinal microbiota. several have been studied for the prevention of aad. five meta-analyses of trials of probiotics for the prevention of aad have been performed. the results showed an overall reduction in the ... | 2008 | 18542041 |
| prokaryotic microbiota of recycled paper mills with low or zero effluent. | the tendency in the paper industry is to close all water loops to save water. this leads to higher process temperatures and may increase the colloidal and dissolved material in the process circulation. increase of nutrients in the water circuits may favor microbiological growth and fouling. in this paper the chemical and microbial compositions of water circuits and deposits were studied of two closed cycled paper/board mills, one mill totally closed (0 m(3) waste water t(-1)), and the other low ... | 2008 | 18651188 |
| effect of dietary probiotic, bacillus coagulans, on growth performance, chemical composition, and meat quality of guangxi yellow chicken. | the effect of bacillus coagulans zju0616 with different concentrations supplemented as probiotic was evaluated on growth performance, feed conversion ratio, survival rate, breast chemical composition, and meat quality of guangxi yellow chicken. birds were randomly segregated into 12 groups so that 3 replicates were available for each of the 3 treatments (t-1, t-2, and t-3) and control groups. the control groups were fed a basal diet without any probiotic and other groups were fed the diets that ... | 2010 | 20181878 |
| synthesis of 4-nitrophenyl acetate using molecular sieve-immobilized lipase from bacillus coagulans. | extracellular lipase from bacillus coagulans bts-3 was immobilized on (3 a x 1.5 mm) molecular sieve. the molecular sieve showed approximately 68.48% binding efficiency for lipase (specific activity 55 iu mg(-1)). the immobilized enzyme achieved approx 90% conversion of acetic acid and 4-nitrophenol (100 mm each) into 4-nitrophenyl acetate in n-heptane at 65 degrees c in 3 h. when alkane of c-chain length other than n-heptane was used as the organic solvent, the conversion of 4-nitrophenol and a ... | 2009 | 19104860 |
| thermophilic bacillus coagulans requires less cellulases for simultaneous saccharification and fermentation of cellulose to products than mesophilic microbial biocatalysts. | ethanol production from lignocellulosic biomass depends on simultaneous saccharification of cellulose to glucose by fungal cellulases and fermentation of glucose to ethanol by microbial biocatalysts (ssf). the cost of cellulase enzymes represents a significant challenge for the commercial conversion of lignocellulosic biomass into renewable chemicals such as ethanol and monomers for plastics. the cellulase concentration for optimum ssf of crystalline cellulose with fungal enzymes and a moderate ... | 2009 | 19156365 |
| effects of the high pressure of homogenization on some spoiling micro-organisms, representative of fruit juice microflora, inoculated in saline solution. | this study was aimed to investigate the effects of a high-pressure homogenization (hph) treatment on some micro-organisms, involved in the spoilage of fruit juices. | 2009 | 19196445 |
| safety assessment of a proprietary preparation of a novel probiotic, bacillus coagulans, as a food ingredient. | it has been demonstrated that some strains of bacillus coagulans can survive extremes of heat, acidity of the stomach, and bile acids, to which commonly consumed probiotics are susceptible. a toxicological safety assessment was performed on a proprietary preparation of b. coagulans - ganedenbc(30) - a novel probiotic. seven toxicologic studies were conducted and included: in vitro bacterial reverse mutation assay; in vitro chromosomal aberration assay; micronucleus assay in mice; acute and 90 da ... | 2009 | 19248815 |
| a patented strain of bacillus coagulans increased immune response to viral challenge. | viral respiratory tract infection is the most common illness among humans. probiotics have been known to enhance the immune system and, therefore, may represent a significant therapeutic advancement for treating viral respiratory tract infections. | 2009 | 19332969 |
| bacillus coagulans significantly improved abdominal pain and bloating in patients with ibs. | symptoms of irritable bowel syndrome (ibs) can have a profound impact on emotional health and quality of life, and current treatments are sometimes unsatisfactory for patients facing this lifelong disease. probiotics, which can normalize gastrointestinal microflora, may alleviate symptoms of ibs. | 2009 | 19332970 |
| inactivation of food spoilage microorganisms by hydrodynamic cavitation to achieve pasteurization and sterilization of fluid foods. | hydrodynamic cavitation is the formation of gas bubbles in a fluid due to pressure fluctuations induced by mechanical means. various high-acid (ph < [corrected]/= 4.6) fluid foods were processed in a hydrodynamic cavitation reactor to determine if commercial sterility can be achieved at reduced processing temperatures. sporicidal properties of the process were also tested on a low-acid (ph > [corrected] 4.6) fluid food. fluid foods were pumped under pressure into a hydrodynamic cavitation reacto ... | 2007 | 18034736 |
| use of a continuous culture fermentation system to investigate the effect of ganedenbc30 (bacillus coagulans gbi-30, 6086) supplementation on pathogen survival in the human gut microbiota. | single-stage continuous fermentation systems were employed to examine the effects of ganedenbc(30) supplementation on the human gastrointestinal microbiota in relation to pathogen challenge in vitro. denaturing gradient gel electrophoresis analysis demonstrated that ganedenbc(30) supplementation modified the microbial profiles in the fermentation systems compared with controls, with profiles clustering according to treatment. overall, ganedenbc(30) supplementation did not elicit major changes in ... | 2010 | 21195203 |
| strong and consistently synergistic inactivation of spores of spoilage-associated bacillus and geobacillus spp. by high pressure and heat compared with inactivation by heat alone. | the inactivation of spores of four low-acid food spoilage organisms by high pressure thermal (hpt) and thermal-only processing was compared on the basis of equivalent thermal lethality calculated at a reference temperature of 121.1°c (f(z)(121.1)(°)(c, 0.1 mpa or 600 mpa)) and characterized as synergistic, not different or protective. in addition, the relative resistances of spores of the different spoilage microorganisms to hpt processing were compared. processing was performed and inactivation ... | 2011 | 21278265 |
| a mixed-species microarray for identification of food spoilage bacilli. | failure of food preservation is frequently caused by thermostable spores of members of the bacillaceae family, which show a wide spectrum of resistance to cleaning and preservation treatments. we constructed and validated a mixed-species genotyping array for 6 bacillus species, including bacillus subtilis, bacillus licheniformis, bacillus pumilus, bacillus sporothermodurans, bacillus cereus and bacillus coagulans, and 4 geobacillus species, including geobacillus stearothermophilus, geobacillus t ... | 2010 | 21315980 |
| randomised controlled clinical trial of lactobacillus sporogenes (bacillus coagulans), used as probiotic in clinical practice, on acute watery diarrhoea in children. | to assess the clinical efficacy of lactobacillus sporogenes (bacillus coagulans), as probiotic preparation, against dehydrating diarrhoea in children. | 2011 | 21332891 |
| effect of treatment with probiotics as water additives on tilapia (oreochromis niloticus) growth performance and immune response. | a feeding trial was conducted for 40 days to delineate the effect of treatment with probiotics as water additives on tilapia (oreochromis niloticus) growth performance and immune response. about 360 juveniles were randomly distributed into four treatment groups, each with three replicates. different probiotics (t-1, bacillus subtilis b10; t-2, bacillus coagulans b16; t-3, rhodopseudomonas palustris g06) were added to the water of tanks at final concentration of 1 x 10(7) cfu ml(-1) every 2 days, ... | 2010 | 19363655 |
| bacillus acidiproducens sp. nov., vineyard soil isolates that produce lactic acid. | two novel spore-forming lactic acid bacteria, strains sl213t and sl1213, were isolated from vineyard soils in korea. cells of both isolates were rod-shaped bacilli and contained subterminal, ellipsoidal spores. strains were facultatively anaerobic, catalase-positive, oxidase-negative and motile with single flagella. meso-diaminopimelic acid, glucose and galactose were detected in whole-cell hydrolysates. major fatty acids found in the strains were anteiso-c15:0, iso-c15:0, iso-c16:0, c16:0 and a ... | 2009 | 19605722 |
| influence of ph on the sonolysis of ciprofloxacin: biodegradability, ecotoxicity and antibiotic activity of its degradation products. | the presence of antibiotics in the aquatic environment has raised concerns due to the potential risk for the emergence or persistence of antibiotic resistance. antibiotics are often poorly degraded in conventional wastewater treatment plants. in this study, sonolysis at 520 khz and 92 w l(-1) was used for the degradation of the fluoroquinolone antibiotic ciprofloxacin. in a first experiment at ph 7, 57% of the ciprofloxacin (15 mg l(-1)) was degraded after 120 min of ultrasonic irradiation at 25 ... | 2009 | 19683328 |
| administration of bacillus coagulans in calves: recovery from faecal samples and evaluation of functional aspects of spores. | an investigation was carried out into the recovery from calf faeces of bacillus coagulans spores added to the feed as probiotic. for this purpose, bacillus coagulans spores (9 log₁₀ cfu g⁻¹) were given daily to 10 calves during the whole farming periods; another 10 calves acted as controls. throughout the trial the faecal spore counts were significantly (p < 0.01) higher in the treated group than in the controls (averaging 2.1 x 10⁵ vs 3.7 x 10⁴ cfu g⁻¹). bacterial cells were recovered from faec ... | 2009 | 19763863 |
| combination effect of ph and acetate on enzymatic cellulose hydrolysis. | the productivity and efficiency of cellulase are significant in cellulose hydrolysis. with the accumulation of volatile fatty acids (vfas), the ph value in anaerobic digestion system is reduced. therefore, this study will find out how the ph and the amount of acetate influence the enzymatic hydrolysis of cellulose. the effects of ph and acetate on cellulase produced from bacillus coagulans were studied at various ph 5-8, and acetate concentrations (0-60 mmol/l). a batch kinetic model for enzymat ... | 2009 | 19862964 |
| growth condition and bacterial community for maximum hydrolysis of suspended organic materials in anaerobic digestion of food waste-recycling wastewater. | this paper reports the effects of changing ph (5-7) and temperature (t, 40-60 degrees c) on the efficiencies of bacterial hydrolysis of suspended organic matter (som) in wastewater from food waste recycling (fwr) and the changes in the bacterial community responsible for this hydrolysis. maximum hydrolysis efficiency (i.e., 50.5% reduction of volatile suspended solids) was predicted to occur at ph 5.7 and t = 44.5 degrees c. changes in short-chain volatile organic acid profiles and in acidogenic ... | 2010 | 19894044 |
| antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium. | probiotic bacteria synthesize extracellular polysaccharides (epss) with commercially significant physiological and therapeutic activities. this important class of biomolecules is also characterized by their ability to remove reactive oxygen species (ros) that are formed in the intestine by various metabolic reactions; hence, they exhibit antioxidant activities. our probiotic bacterium, bacillus coagulans rk-02, produces an eps during the exponential and stationary growth phases when grown in a g ... | 2008 | 18246578 |
| a prospective, randomized, double-blind, placebo-controlled parallel-group dual site trial to evaluate the effects of a bacillus coagulans-based product on functional intestinal gas symptoms. | this randomized double blind placebo controlled dual site clinical trial compared a probiotic dietary supplement to placebo regarding effects on gastrointestinal symptoms in adults with post-prandial intestinal gas-related symptoms (abdominal pain, distention, flatulence) but no gastrointestinal (gi) diagnoses to explain the symptoms. | 2009 | 19922649 |
| isolation and characterization of hyper phenol tolerant bacillus sp. from oil refinery and exploration sites. | bacillus cereus mtcc 9817 strain akg1 and b. cereus mtcc 9818 strain akg2 were isolated from petroleum refinery and oil exploration site, respectively. the 16s rdna sequence of strain akg1 showed the closest relation to b. cereus 99.63% and bacillus coagulans 99.63% followed by 99.34% homology with bacillus thuringiensis strain 2pr56-10. akg2 is mostly related to b. thuringiensis strain cmg 861 with 99.37% homology. the similarity search between akg1 and akg2 gave the lowest similarity 99.19% am ... | 2010 | 19945784 |
| [effects of microbial agent inoculation on bacterial community diversity in the process of pig manure composting]. | pcr-dgge method was adopted to study the effects of inoculating exogenous microbial agent on the bacterial community diversity in the process of fresh pig manure high-temperature aerobic composting. exogenous microbial agent inoculation promoted the composting process, with the high-temperature period being advanced by 2 days than that of non-inoculation. dgge pattern analysis showed that during composting, the dominant bacteria changed significantly, with the shannon-wiener index of bacterial c ... | 2009 | 19947226 |
| national survey outcomes on commercial probiotic food supplements in italy. | to assess whether the probiotic food supplements, produced and distributed on the italian market during 2005-2006, complied with the italian guidelines on prebiotics and probiotics, 72 samples from 29 processing plants were analyzed. the survey included 41 samples from processing plants and 31 samples of the same brand from retailers collected at timed intervals (3, 8 and 13 months). a polyphasic approach based on a suitable analytical collection method (genotypic identification of total bacteri ... | 2010 | 20061041 |
| effects of a proprietary bacillus coagulans preparation on symptoms of diarrhea-predominant irritable bowel syndrome. | symptoms of irritable bowel syndrome (ibs) have a profound impact on quality of life for many patients and current treatments are sometimes unsatisfactory. this controlled pilot study was conducted to evaluate effects of the proprietary ganedenbc(30) (bacillus coagulans gbi-30, 6086) probiotic on ibs symptoms, in a randomized, double-blind, placebo-controlled clinical trial including patients with diarrhea-predominant ibs (ibs-d). patients were randomized to receive either b. coagulans gbi-30, 6 ... | 2009 | 20140275 |
| fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile bacillus coagulans. | a strain of bacillus coagulans that converted mixed sugars of glucose, xylose, and arabinose to l: -lactic acid with 85% yield at 50 degrees c was isolated from composted dairy manure. the strain was tolerant to aldehyde growth inhibitors at 2.5 g furfural/l, 2.5 g 5-hydroxymethylfurfural/l, 2.5 g vanillin/l, and 1.2 g p-hydroxybenzaldehyde/l. in a simultaneous saccharification and fermentation process, the strain converted a dilute-acid hydrolysate of 100 g corn fiber/l to 39 g lactic acid/l in ... | 2010 | 20155485 |
| microbial load and antimicrobial property of two nigerian herbal remedies. | the use of herbal remedies had increased significantly in the last one decade in nigeria. this has led to the production of herbal products with bogus claims. the microbial quality and antibacterial properties of two nigerian herbal remedies with such claimed efficacy of curing all manners of microbial diseases were assessed. the herbal remedies were discovered to be contaminated with the following microorganisms: bacillus subtilis, bacillus coagulans, bacillus cereus, basidiobotrytis sp, oedoce ... | 2007 | 20162058 |
| Genome sequence of the thermophilic strain Bacillus coagulans XZL4, an efficient pentose-utilizing producer of chemicals. | Bacillus coagulans XZL4 is an efficient pentose-utilizing producer of important platform compounds, such as l-lactic acid, 2,3-butanediol, and acetoin. Here we present a 2.8-Mb assembly of its genome. Simple and efficient carbohydrate metabolism systems, especially the transketolase/transaldolase pathway, make it possible to convert pentose sugars to products at high levels. | 2011 | 22038963 |
| ganedenbc30 cell wall and metabolites: anti-inflammatory and immune modulating effects in vitro. | this study was performed to evaluate anti-inflammatory and immune modulating properties of the probiotic, spore-forming bacterial strain: bacillus coagulans: gbi-30, (pta-6086, ganedenbc30tm). in addition, cell wall and metabolite fractions were assayed separately to address whether biological effects were due to cell wall components only, or whether secreted compounds from live bacteria had additional biological properties. the spores were heat-activated, and bacterial cultures were grown. the ... | 2010 | 20331905 |
| effect of probiotic on growth performance and digestive enzyme activity of arbor acres broilers. | the effect of probiotic, bacillus coagulans nj0516, on growth performance and digestive enzyme activity of arbor acres (aa) broilers was investigated. strains nj0516 were added to commercial basal diets as probiotic at three final concentrations: t-1, 1x10(6) cfu g(-1); t-2, 2x10(6) cfu g(-1) and t-3, 4x10(6) cfu g(-1), respectively. twelve groups, of 30 broilers, with three replicates for each treatment group (t-1, t-2 and t-3) and the control group treated without probiotic were used. after 49 ... | 2010 | 20350733 |
| Antibacterial activity of different degree of hydrolysis of palm kernel expeller peptides against spore-forming and non-spore-forming bacteria. | The goal of this study was to determine inhibitory effect of palm kernel expeller (PKE) peptides of different degree of hydrolysis (DH %) against spore-forming bacteria Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophillus, Bacillus subtilis, Bacillus thuringiensis, Clostridium perfringens; and non-spore-forming bacteria Escherichia coli, Lisinibacillus sphaericus, Listeria monocytogenes, Pseudomonas a ... | 2011 | 21848644 |
| biochemical properties and yields of diverse bacterial laccase-like multicopper oxidases expressed in escherichia coli. | laccases are multi-copper oxidases that oxidize a broad range of substrates at the expense of molecular oxygen, without any need for co-factor regeneration. these enzymes bear high potential for the sustainable synthesis of fine chemicals and the modification of (bio)polymers. here we describe cloning and expression of five novel bacterial laccase-like multi copper oxidases (lmcos) of diverse origin which were identified by homology searches in online databases. activity yields under different e ... | 2015 | 26068013 |
| genetic tool development for a new host for biotechnology, the thermotolerant bacterium bacillus coagulans. | bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. here, we present a targeted gene disruption system using the cre-lox system, development of a lacz reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression. | 2010 | 20400555 |
| production of lactic acid from hemicellulose extracts by bacillus coagulans mxl-9. | bacillus coagulans mxl-9 was found capable of growing on pre-pulping hemicellulose extracts, utilizing all of the principle monosugars found in woody biomass. this organism is a moderate thermophile isolated from compost for its pentose-utilizing capabilities. it was found to have high tolerance for inhibitors such as acetic acid and sodium, which are present in pre-pulping hemicellulose extracts. fermentation of 20 g/l xylose in the presence of 30 g/l acetic acid required a longer lag phase but ... | 2010 | 20454831 |
| the effects of orally administered bacillus coagulans and inulin on prevention and progression of rheumatoid arthritis in rats. | probiotics have been considered as an approach to addressing the consequences of different inflammatory disorders. the spore-forming probiotic strain bacillus coagulans has demonstrated anti-inflammatory and immune-modulating effects in both animals and humans. the prebiotic inulin also potentially affects the immune system as a result of the change in the composition or fermentation profile of the gastrointestinal microbiota. | 2016 | 27427194 |
| bacteremia in patients with acute pancreatitis as revealed by 16s ribosomal rna gene-based techniques*. | to define the characteristic of bacteremia in patients with acute pancreatitis and determine its possible association with the disease severity. | 2013 | 23863226 |
| in vitro evaluation of the antimicrobial activity of eugenol, limonene, and citrus extract against bacteria and yeasts, representative of the spoiling microflora of fruit juices. | this article reports on the investigation on the bioactivity of eugenol, limonene, and citrus extract against three bacteria (lactobacillus plantarum, lactobacillus brevis, and bacillus coagulans) and three yeasts (saccharomyces bayanus, pichia membranifaciens, and rhodotorula bacarum), representing the spoilage microflora of fruit juices. the experiments were performed with laboratory media by using a microdilution method. data were fitted using the gompertz equation, and the kinetic parameters ... | 2010 | 20501040 |
| physiological and fermentation properties of bacillus coagulans and a mutant lacking fermentative lactate dehydrogenase activity. | bacillus coagulans, a sporogenic lactic acid bacterium, grows optimally at 50-55 °c and produces lactic acid as the primary fermentation product from both hexoses and pentoses. the amount of fungal cellulases required for simultaneous saccharification and fermentation (ssf) at 55 °c was previously reported to be three to four times lower than for ssf at the optimum growth temperature for saccharomyces cerevisiae of 35 °c. an ethanologenic b. coagulans is expected to lower the cellulase loading a ... | 2011 | 20677017 |
| l: (+)-lactic acid production from non-food carbohydrates by thermotolerant bacillus coagulans. | lactic acid is used as an additive in foods, pharmaceuticals, and cosmetics, and is also an industrial chemical. optically pure lactic acid is increasingly used as a renewable bio-based product to replace petroleum-based plastics. however, current production of lactic acid depends on carbohydrate feedstocks that have alternate uses as foods. the use of non-food feedstocks by current commercial biocatalysts is limited by inefficient pathways for pentose utilization. b. coagulans strain 36d1 is a ... | 2011 | 20694852 |
| α-glucosylated 6-gingerol: chemoenzymatic synthesis using α-glucosidase from halomonas sp. h11, and its physical properties. | 6-gingerol [(s)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)decan-3-one] is a biologically active compound and is abundant in the rhizomes of ginger (zingiber officinale). it has some beneficial functions in healthcare, but its use is limited because of its insolubility in water and its heat-instability. to improve these physical properties, the glucosylation of 6-gingerol was investigated using α-glucosidases (ec. 3.2.1.20) from aspergillus niger, aspergillus nidulans abpu1, acremonium strictum, hal ... | 2012 | 22537860 |
| one-year chronic oral toxicity with combined reproduction toxicity study of a novel probiotic, bacillus coagulans, as a food ingredient. | some strains of bacillus coagulans can survive extremes of heat, stomach acid and bile acids, to which commonly consumed probiotics are susceptible. a toxicological safety assessment was published in 2009 on a proprietary preparation of b. coagulans - ganedenbc(30)™ - a novel probiotic. it was concluded that ganedenbc(30)™ is safe for chronic human consumption based upon scientific procedures, supported by a safe history of use (endres et al., 2009). a one-year chronic oral toxicity study combin ... | 2011 | 21338652 |
| differential oxidative stress responses in castor semilooper, achaea janata. | balance between reactive oxygen species (ros) and the antioxidant (ao) defense mechanisms is vital for organism survival. insects serve as an ideal model to elucidate oxidative stress responses as they are prone to different kinds of stress during their life cycle. the present study demonstrates the modulation of ao enzyme gene expression in the insect pest, achaea janata (castor semilooper), when subjected to different oxidative stress stimuli. antioxidant enzymes' (catalase (cat), superoxide d ... | 2015 | 26455997 |
| purification and characterization of a novel plantaricin, kl-1y, from lactobacillus plantarum kl-1. | three bacteriocins from lactobacillus plantarum kl-1 were successfully purified using ammonium sulfate precipitation, cation-exchange chromatography and reverse-phase hplc. the bacteriocin peptides kl-1x, -1y and -1z had molecular masses of 3053.82, 3498.16 and 3533.16 da, respectively. all three peptides were stable at ph 2-12 and 25 °c and at high temperatures of 80 and 100 °c for 30 min and 121 °c for 15 min. however, they differed in their susceptibility to proteolytic enzymes and their inhi ... | 2015 | 25862353 |
| germination and inactivation of bacillus coagulans and alicyclobacillus acidoterrestris spores by high hydrostatic pressure treatment in buffer and tomato sauce. | acidothermophilic bacteria like alicyclobacillus acidoterrestris and bacillus coagulans can cause spoilage of heat-processed acidic foods because they form spores with very high heat resistance and can grow at low ph. the objective of this work was to study the germination and inactivation of a. acidoterrestris and b. coagulans spores by high hydrostatic pressure (hp) treatment at temperatures up to 60°c and both at low and neutral ph. in a first experiment, spores suspended in buffers at ph 4.0 ... | 2011 | 21421274 |
| efficient conversion of phenylpyruvic acid to phenyllactic acid by using whole cells of bacillus coagulans sdm. | phenyllactic acid (pla), a novel antimicrobial compound with broad and effective antimicrobial activity against both bacteria and fungi, can be produced by many microorganisms, especially lactic acid bacteria. however, the concentration and productivity of pla have been low in previous studies. the enzymes responsible for conversion of phenylpyruvic acid (ppa) into pla are equivocal. | 2011 | 21533054 |
| screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts. | the selection of promising specific species of lactic acid bacteria with potential probiotic characteristics is of particular interest in producing multi species-specific probiotic adjuncts in veal calves rearing. the aim of the present work was to select and evaluate in vitro the functional activity of lactic acid bacteria, bifidobacterium longum and bacillus coagulans strains isolated from veal calves in order to assess their potential use as multi species-specific probiotics for veal calves. ... | 2011 | 21619939 |
| formulation and evaluation of bacillus coagulans-loaded hypromellose mucoadhesive microspheres. | development of a novel delivery system has been attempted to deliver viable probiotic cells into the gut for a prolonged period of time while maintaining high numbers of viable cells within the formulation throughout the shelf-life of the product and during the gastrointestinal transit. core mucoadhesive microspheres of bacillus coagulans were developed employing several grades of hypromellose, a mucoadhesive polymer, following coacervation and phase separation technique and were subsequently en ... | 2011 | 21674019 |
| a brief dataset on the model-based evaluation of the growth performance of bacillus coagulans and l-lactic acid production in a lignin-supplemented medium. | the data presented in this article are related to the research article entitled "model-based characterization of growth performance and l-lactic acid production with high optical purity by thermophilic bacillus coagulans in a lignin-supplemented mixed substrate medium (r. glaser and j. venus, 2016) [1]". this data survey provides the information on characterization of three bacillus coagulans strains. information on cofermentation of lignocellulose-related sugars in lignin-containing media is gi ... | 2017 | 28243619 |
| in vitro evaluation of bioremediation capacity of a commercial probiotic, bacillus coagulans, for chromium (vi) and lead (ii) toxicity. | the bioaccumulation of heavy metals including chromium (vi) (cr (vi)) and lead (ii) (pb (ii)) causes fatal toxicity in humans. some naturally occurring bacterial genera such as bacillus and pseudomonas help in bioremediation of these heavy metals and some of the species of bacillus are proven probiotics. however, no study has been conducted on bacillus coagulans, which is a proven probiotic species of genus bacillus. | 2017 | 28216949 |
| erratum to: effect of probiotic bacillus coagulans and lactobacillus plantarum on alleviation of mercury toxicity in rat. | | 2017 | 28205137 |
| the effects of bacillus coagulans-fermented and non-fermented ginkgo biloba on abdominal fat deposition and meat quality of peking duck. | | 2017 | 28204698 |
| model-based characterisation of growth performance and l-lactic acid production with high optical purity by thermophilic bacillus coagulans in a lignin-supplemented mixed substrate medium. | three bacillus coagulans strains were characterised in terms of their ability to grow in lignin-containing fermentation media and to consume the lignocellulose-related sugars glucose, xylose, and arabinose. an optical-density high-throughput screening was used for precharacterisation by means of different mathematical models for comparison (logistic, gompertz, baranyi, richards & stannard, and schnute). the growth response was characterised by the maximum growth rate and lag time. for a comparis ... | 2017 | 28188935 |
| a comparative evaluation of probiotics on salivary mutans streptococci counts in indian children. | probiotics and their effect on oral health have been a focus of numerous trials in recent times. no documented trials have been reported from developing countries such as india with its focus on probiotic use, especially in the paediatric population. the aim of this study is to evaluate the effect of probiotics on salivary mutans streptococci (ms) counts of children using the two commercially and widely available preparations and to explore their anti-caries potential. | 2011 | 21806906 |
| effect of probiotic bacillus coagulans and lactobacillus plantarum on alleviation of mercury toxicity in rat. | the objective of this study was to evaluate the efficiency of probiotics (lactobacillus plantarum and bacillus coagulans) against mercury-induced toxicity using a rat model. mercury (hg) is a widespread heavy metal and was shown to be associated with various diseases. forty-eight adult male wistar rats were randomly divided into six groups (control, mercury-only, each probiotic-only, and mercury plus each probiotic group). hg-treated groups received 10 ppm mercuric chloride, and probiotic groups ... | 2017 | 28084611 |
| production of l(+)-lactic acid from acid pretreated sugarcane bagasse using bacillus coagulans dsm2314 in a simultaneous saccharification and fermentation strategy. | sugars derived from lignocellulose-rich sugarcane bagasse can be used as feedstock for production of l(+)-lactic acid, a precursor for renewable bioplastics. in our research, acid-pretreated bagasse was hydrolysed with the enzyme cocktail gc220 and fermented by the moderate thermophilic bacterium bacillus coagulans dsm2314. saccharification and fermentation were performed simultaneously (ssf), adding acid-pretreated bagasse either in one batch or in two stages. ssf was performed at low enzyme do ... | 2016 | 27872661 |
| purification and partial elucidation of the structure of an antioxidant carbohydrate biopolymer from the probiotic bacterium bacillus coagulans rk-02. | an exopolysaccharide (eps) was isolated from bacillus coagulans rk-02 and purified by size exclusion chromatography. the purified, homogeneous eps had an average molecular weight of ~3 × 10(4) da by comparison with fitc-labeled dextran standards. in vivo evaluations showed that, like other reported polysaccharides, this eps displayed significant antioxidant activity. ftir spectroscopy analysis showed the presence of hydroxy, carboxy, and a-glycosidic linkages and a mannose residue. gc analysis i ... | 2011 | 21800834 |
| cost-effective simultaneous saccharification and fermentation of l-lactic acid from bagasse sulfite pulp by bacillus coagulans cc17. | the main barriers to cost-effective lactic acid production from lignocellulose are the high cost of enzymes and the ineffective utilization of the xylose within the hydrolysate. in the present study, the thermophilic bacillus coagulans strain cc17 was used for the simultaneous saccharification and fermentation (ssf) of bagasse sulfite pulp (bsp) to produce l-lactic acid. unexpectedly, ssf by cc17 required approximately 33.33% less fungal cellulase than did separate hydrolysis and fermentation (s ... | 2016 | 27750196 |
| survival and metabolic activity of the ganedenbc30 strain of bacillus coagulans in a dynamic in vitro model of the stomach and small intestine. | we have investigated the survival and activity of ganedenbc30 during passage through the upper gastro-intestinal tract. ganedenbc30 was tested in a dynamic, validated, in vitro model of the stomach and small intestine (tim-1) on survival and its potential to aid in digestion of milk protein, lactose and fructose. the survival of ganedenbc30 was high (70%), although germination of the spores was minimal (<10%) under the conditions tested. survival of the strain in the presence of lactose and fruc ... | 2010 | 21831748 |
| development and evaluation of intestinal targeted mucoadhesive microspheres of bacillus coagulans. | background: intestinal targeted mucoadhesive microsphere of probiotics may provide numerous associated health benefits. aim: to develop mucoadhesive microspheres that will deliver viable probiotic cells into gut protectively against harsh environmental conditions of stomach for extended period. materials and methods: core mucoadhesive microspheres of bacillus coagulans were prepared using hypromellose, following coacervation and phase separation technique and were then coated with hypromellose p ... | 2011 | 21702732 |
| bacillus coagulans: a viable adjunct therapy for relieving symptoms of rheumatoid arthritis according to a randomized, controlled trial. | lactic acid-producing bacteria (lab) probiotics demonstrate immunomodulating and anti-inflammatory effects and the ability to lessen the symptoms of arthritis in both animals and humans. this randomized, double-blind, placebo-controlled, parallel-design, clinical pilot trial was conducted to evaluate the effects of the lab probiotic preparation, bacillus coagulans gbi-30, 6086, on symptoms and measures of functional capacity in patients with rheumatoid arthritis (ra) in combination with pharmaco ... | 2010 | 20067641 |
| ciprofloxacin ozonation in hospital wastewater treatment plant effluent: effect of ph and h2o2. | the ozonation of ciprofloxacin was studied in hospital wastewater treatment plant effluent with focus on parent compound degradation, degradation product identification and residual antibacterial activity. before ozonation, ciprofloxacin sorption on suspended solids was tested as a function of temperature (10.0-27.5 degrees c) and ph (3, 7 and 10). temperature did not significantly affect ciprofloxacin sorption while sorption was highest at ph 7 (logk(d)=4.7) compared to ph 3 (logk(d)=4.3) and 1 ... | 2010 | 20074775 |
| kinetic model of thermophilic l-lactate fermentation by bacillus coagulans combined with real-time pcr quantification. | a simple l-lactate fermentation of organic wastes at ph 5.5 and 55 degrees c under nonsterile conditions using bacillus coagulans can be suitable for l-lactate fermentation of garbage. a mathematical model that simulated the lactate fermentation characteristics of b. coagulans was developed by focusing on the inhibitory effects of substrate, lactate (product) and nacl, and bacterial growth. basic fermentation experiments were performed using simple substrates to derive fundamental parameters of ... | 2010 | 20122710 |
| Characterization and identification of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13. | Aims: To identify and characterize novel bacteriocins from Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13, isolated from a barrel used to make Japanese pickles, produced two novel bacteriocins termed weissellicin Y and weissellicin M. The primary structures of weissellicins Y and M were determined, and their molecular masses were determined to be 4925·12 and 4968·40 Da, respectively. Analysis of the DNA sequence encoding the bacteriocins revealed that they were synth ... | 2012 | 22008177 |
| Evolution of D-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for D-lactate production from lignocellulose. | Lactic acid, an attractive, renewable chemical for production of biobased plastics (polylactic acid, PLA), is currently commercially produced from food-based sources of sugar. Pure optical isomers of lactate needed for PLA are typically produced by microbial fermentation of sugars at temperatures below 40?°C. Bacillus coagulans produces L(+)-lactate as a primary fermentation product and grows optimally at 50?°C and pH 5, conditions that are optimal for activity of commercial fungal cellulases. T ... | 2011 | 22065761 |
| bacillus coagulans gbi-30 (bc30) improves indices of clostridium difficile-induced colitis in mice. | abstract: | 2011 | 22014083 |
| kinetic modeling of sporulation and product formation in stationary phase by bacillus coagulans rk-02 vis-à-vis other bacilli. | a logistic kinetic model was derived and validated to characterize the dynamics of a sporogenous bacterium in stationary phase with respect to sporulation and product formation. the kinetic constants as determined using this model are particularly important for describing intrinsic properties of a sporogenous bacterial culture in stationary phase. non-linear curve fitting of the experimental data into the mathematical model showed very good correlation with the predicted values for sporulation a ... | 2011 | 21852126 |
| contributory roles of two l-lactate dehydrogenases for l-lactic acid production in thermotolerant bacillus coagulans. | thermotolerant bacillus coagulans is considered to be a more promising producer for bio-chemicals, due to its capacity to withstand harsh conditions. two l-lactate dehydrogenase (ldh) encoding genes (ldhl1 and ldhl2) and one d-ldh encoding gene (ldhd) were annotated from the b. coagulans dsm1 genome. transcriptional analysis revealed that the expression of ldhl2 was undetectable while the ldhl1 transcription level was much higher than that of ldhd at all growth phases. deletion of the ldhl2 gene ... | 2016 | 27885267 |
| lactic acid production from pretreated hydrolysates of corn stover by a newly developed bacillus coagulans strain. | an inhibitor-tolerance strain, bacillus coagulans gkn316, was developed through atmospheric and room temperature plasma (artp) mutation and evolution experiment in condensed dilute-acid hydrolysate (cdh) of corn stover. the fermentabilities of other hydrolysates with b. coagulans gkn316 and the parental strain b. coagulans nl01 were assessed. when using condensed acid-catalyzed steam-exploded hydrolysate (caseh), condensed acid-catalyzed liquid hot water hydrolysate (calh) and condensed acid-cat ... | 2016 | 26863012 |
| bacteremia in the patients with acute pancreatitis as revealed by 16s ribosomal rna gene-based techniques. | objectives:: to define the characteristic of bacteremia in the patients with acute pancreatitis and determine its possible association with the disease severity. design:: a prospective controlled study. setting:: icu of jinling hospital, china. patients:: a total of 48 patients with mild or severe acute pancreatitis were enrolled in this study. interventions:: none. measurements and main results:: samples of peripheral blood were collected from the patients at 4 or 5 and 9 or 10 days after acute ... | 2013 | 23764972 |
| two highly adhesive lactic acid bacteria strains are protective in zebrafish infected with aeromonas hydrophila by evocation of gut mucosal immunity. | to increase the knowledge of probiotic effects and potential mechanisms, we report on the use of the zebrafish model to investigate the in vivo colonization ability, as well as the protective effects associated with gut mucosal immune barrier and responses against aeromonas hydrophila infection of previously characterized probiotic lactic acid bacteria (lab) strains, bacillus coagulans 09·712 and lactobacillus plantarum 08·923, in comparison with that of three commercialized strains. | 2016 | 26555459 |
| dietary supplementation of probiotics affects growth, immune response and disease resistance of cyprinus carpio fry. | the effects of dietary bacillus coagulans (mtcc 9872), bacillus licheniformis (mtcc 6824) and paenibacillus polymyxa (mtcc 122) supplementation on growth performance, non-specific immunity and protection against aeromonas hydrophila infection were evaluated in common carp, cyprinus carpio fry. laboratory maintained b. coagulans, b. licheniformis and p. polymyxa were used to study antagonistic activity against fish pathogenic bacteria by agar well diffusion assay. healthy fish fry were challenged ... | 2014 | 25160796 |
| effects of probiotic supplementation in different nutrient density diets on growth performance, nutrient digestibility, blood profiles, fecal microflora and noxious gas emission in weaning pig. | probiotics can serve as alternatives to antibiotics to increase the performance of weaning pigs, and the intake of probiotics is affected by dietary nutrient density. the objective of this study was to evaluate the effects of a probiotic complex in different nutrient density diets on growth performance, digestibility, blood profiles, fecal microflora and noxious gas emission in weaning pigs. | 2017 | 27342952 |
| genome sequences of 12 spore-forming bacillus species, comprising bacillus coagulans, bacillus licheniformis, bacillus amyloliquefaciens, bacillus sporothermodurans, and bacillus vallismortis, isolated from foods. | here, we report the draft genomes of twelve isolates of five different bacillus species, all spore-forming, gram-positive bacteria. | 2016 | 27174261 |
| inactivation of bacillus spores inoculated in milk by ultra high pressure homogenization. | ultra high-pressure homogenization treatments at 300 mpa with inlet temperatures (ti) of 55, 65, 75 and 85 °c were applied to commercial ultra high temperature treated whole milk inoculated with bacillus cereus, bacillus licheniformis, bacillus sporothermodurans, bacillus coagulans, geobacillus stearothermophilus and bacillus subtilis spores in order to evaluate the inactivation level achieved. ultra high-pressure homogenization conditions at 300 mpa with ti = 75 and 85 °c were capable of a spor ... | 2014 | 25084664 |
| synthesis, characterization and biological studies of schiff bases derived from heterocyclic moiety. | some new schiff bases (h1-h7) have been synthesized by the condensation of 2-aminophenol, 2-amino-4-nitrophenol, 2-amino-4-methylphenol, 2-amino benzimidazole with thiophene-2-carboxaldehyde and pyrrole-2-carboxaldehyde. the structures of newly synthesized compounds were characterized by elemental analysis, ft-ir, (1)h nmr, uv-vis, and single crystal x-ray crystallography. the in vitro antibacterial activity of the synthesized compounds has been tested against salmonella typhi, bacillus coagulan ... | 2017 | 27894775 |
| bacillus species isolated from tungrymbai and bekang, naturally fermented soybean foods of india. | tungrymbai and bekang are naturally fermented soybean foods commonly consumed in meghalaya and mizoram states of india. a total of 39 samples of tungrymbai and 43 samples of bekang were collected from different villages and markets of meghalaya and mizoram, respectively and were analysed for microbial load. in both tungrymbai and bekang, the average population of bacillus spp. was 8.2±0.1 log cfu/g. a total of 428 isolates of bacillus were isolated from tungrymbai (211) and bekang (217) for deta ... | 2015 | 25574846 |
| detoxification of sap from felled oil palm trunks for the efficient production of lactic acid. | the availability of fermentable sugars in high concentrations in the sap of felled oil palm trunks and the thermophilic nature of the recently isolated bacillus coagulans strain 191 were exploited for lactic acid production under non-sterile conditions. screening indicated that strain 191 was active toward most sugars including sucrose, which is a major component of sap. strain 191 catalyzed a moderate conversion of sap sugars to lactic acid (53%) with a productivity of 1.56 g/l/h. pretreatment ... | 2017 | 28361245 |
| waste-to-biofuel: production of biobutanol from sago waste residues. | the main concern of extensive production of biobutanol has been associated with the high cost of the substrate and the relatively low tolerance of clostridia to biobutanol production. in this study, the use of fermentable cassava waste residue (cwr) as substrate for biobutanol production was investigated using solvent-tolerant clostridium sp. four of obligatory, solvent-producing bacteria were isolated from sago industry waste sites. the nsw, pnas1, sb5 and sbi4 strains showed identical profiles ... | 2017 | 28091177 |
| comparison of high-titer lactic acid fermentation from naoh- and nh3-h2o2-pretreated corncob by bacillus coagulans using simultaneous saccharification and fermentation. | lignocellulose is one of the most abundant renewable feedstocks that has attracted considerable attention as a substrate for biofuel and biochemical production. one such biochemical product, lactic acid, is an important fermentation product because of its great potential for the production of biodegradable and biocompatible polylactic acid. high-titer lactic acid production from lignocellulosic materials has been achieved recently; however, it requires biodetoxification or results in large amoun ... | 2016 | 27853308 |
| inhibition of lipopolysaccharide-induced interleukin 8 in human adenocarcinoma cell line ht-29 by spore probiotics: b. coagulans and b. subtilis (natto). | probiotics are used as a treatment for different intestinal disorders. they confer health benefits by different ways. this study was aimed to investigate immunomodulatory effect of bacillus probiotic spores on the production of lipopolysaccharide (lps)-induced interleukin 8 (il-8) in ht-29 intestinal epithelial cells. differentiated intestinal epithelial cell line was used as a model for the study of colonization of purified spores (bacillus subtilis (natto) and b. coagulans) and their anti-infl ... | 2017 | 27785697 |
| statistical optimization for enhanced yields of probiotic bacillus coagulans and its phage resistant mutants followed by kinetic modelling of the process. | probiotics are microorganisms which when administered in adequate amounts confer health benefits to the host. a leading pharmaceutical company producing bacillus coagulans as a probiotic was facing the problem of recurring phage attacks. two mutants viz. b. co piii and b. co miii that were isolated as phage resistant mutants after uv irradiation and mms treatment of phage sensitive b. coagulans parental culture were characterized at functional and molecular level and were noted to have undergone ... | 2016 | 27722071 |
| effect of low ph start-up on continuous mixed-culture lactic acid fermentation of dairy effluent. | mixed-culture fermentation that does not require an energy-intensive sterilization process is a viable approach for the economically feasible production of lactic acid (la) due to the potential use of organic waste as feedstock. this study investigated mixed-culture la fermentation of whey, a high-strength organic wastewater, in continuous mode. variations in the hydraulic retention time (hrt) from 120 to 8 h under different ph regimes in two thermophilic reactors (55 °c) were compared for their ... | 2016 | 27709285 |
| genome sequence of the thermophilic strain bacillus coagulans2-6, an efficient producer of high-optical-purity l-lactic acid. | bacillus coagulans2-6 is an efficient producer of lactic acid. the genome of b. coagulans2-6 has the smallest genome among the members of the genus bacillusknown to date. the frameshift mutation at the start of the d-lactate dehydrogenase sequence might be responsible for the production of high-optical-purity l-lactic acid. | 2011 | 21705584 |
| identification and characterization of leucocyclicin q, a novel cyclic bacteriocin produced by leuconostoc mesenteroides tk41401. | the culture supernatant of leuconostoc mesenteroides tk41401, isolated from japanese pickles, possessed antimicrobial activity against broad range of a bacterial genera and particularly strong activity against bacillus coagulans, the major contaminant of pickles. an antimicrobial peptide was purified in three chromatographic steps, and its molecular mass was determined to be 6,115.59 da by electrospray ionization time-of-flight mass spectrometry (esi-tof ms). the primary structure of this peptid ... | 2011 | 21948835 |
| Applications of ß-gal-III isozyme from Bacillus coagulans RCS3, in lactose hydrolysis. | Bacillus coagulans RCS3 isolated from hot water springs secreted five isozymes i.e. ß-gal I-V of ß-galactosidase. ß-gal III isozyme was purified using DEAE cellulose and Sephadex G 100 column chromatography. Its molecular weight characterization showed a single band at 315kD in Native PAGE, while two subunits of 50.1 and 53.7 kD in SDS PAGE. ß-Gal III had pH optima in the range of 6-7 and temperature optima at 65°C. It preferred nitro-aryl-ß-d-galactoside as substrate having K(m) of 4.16 mM with ... | 2011 | 21855568 |