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oropharyngeal microflora in aboriginal and non-aboriginal australian children. an indicator of environmental contamination.faecal microorganisms, not normally resident in the oropharynx, were isolated much more often from aboriginal than non-aboriginal australian children. this is almost certainly due to differences in standards of hygiene and living in these two groups, and is an important factor in contributing to the common and serious problem of gastrointestinal infections in young aborigines. in the non-aboriginal group, the isolations of faecal organisms were much more common from infants and from children und ...1979117289
[effect of microorganisms on survival of trichomonas vaginalis in physiological fluid]. 1979117638
intestinal microflora: elimination of germfree characteristics by components of the normal microbial flora. 1979117972
the automicrobic system for urines.an evaluation of the automicrobic system (ams) for urines (vitek systems, inc.) was carried out under the auspices of the product evaluation committee of the college of american pathologists from the period june 1977 through october 1978. data generated during this evaluation indicated that, when comparing the ams methodology to our clinical microbiology laboratory methodology, a 37% time saving could be realized by utilizing the ams. quantitation with the ams showed a 99% correlation with the c ...1979118176
the distribution of isoprenoid quinones in streptococci of serological groups d and n.the isoprenoid quinone contents of streptococci of serological groups d and n were investigated. streptococcus faecalis, s. faecalis subsp. liquefaciens and s. faecalis subsp. zymogenes strains contained demethylmenaquinones with nine isoprene units as their major isoprenologues. menaquinones with eight isoprene units predominated in s. faecium subsp. casseliflavus and s. faecium subsp. mobilis whereas menaquinones with nine isoprene units constituted the major components in strains of s. cremor ...1979118232
rural water quality control measures: an experience in the malumfashi district of northern nigeria. 1979118477
[phagocytic ability of trichomonas vaginalis donné]. 1979118590
high-level, plasmid-borne resistance to gentamicin in streptococcus faecalis subsp. zymogenes.each of three isolates of streptococcus faecalis subsp. zymogenes harbored three r plasmids and a hemolysin-bacteriocin plasmid. the plasmids carried by one of these strains were physically characterized after their conjugative transfer. in each strain one of the plasmids carried genetic markers for resistance to gentamicin, kanamycin, sisomicin, netilmicin, and tobramycin.1979118707
the role of liver passage for conversion of pteroylmonoglutamate and pteroyltriglutamate to active folate coenzyme.in an attempt to clarify the possible site of conversion of folates to active methylated coenzyme forms mono- and triglutamate was injected rapidly into the portal vein of dogs. samples of blood were removed at various intervals from catheters placed in the portal vein, the hepatic vein and the femoral artery for assay carried out both with l. casei, s. faecalis and liquid scintillating counting. unreduced monoglutamates were extracted in the liver, immediately converted to methyltetrahydrofolat ...1979118944
the presence of antibody-coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy.quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. the fluorescent antibody (fa) test was applied to the sediments of bladder aspirates to determine the site of infection. anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were fa-positive. these anaerobes wer ...1979118996
[drug resistance of bacteria isolated from patients with chronic nonspecific broncho-pulmonary diseases]. 1979119214
chemiluminescence from eukaryotic and prokaryotic cells: reducing potential and oxygen requirements. 1979119237
[development of enterococci and coli bacteria in bulgarian yogurt].the effect of lactic acid fermentation in bulgarian yoghourt was studied by the development and decline of hygiene level indicating microorganisms--enterococci and coli-bacteriae. it was established that for the first 6 h after production of bulgarian yoghourt, the quantity of enterococci (str. faecalis, resp. str. faecium) increases 5--6 times on the average, after the 12th hour it begins slowly to decrease and the number is reduced two times during storage for 7 days at 7--10 degrees c tempera ...1979119346
kinetic studies on enzymatic acetylation of chloramphenicol in streptococcus faecalis.the kinetics of chloramphenicol (cp) acetylation by cp acetyltransferase from streptococcus faecalis was studied. cp was shown to be acetylated enzymatically to its 3-o-acetyl derivative (3-accp) in the presence of acetyl coenzyme a, after which 3-accp was converted nonenzymatically to its 1-o-acetyl isomer, 1-o-acetyl cp (1-accp). at equilibrium, the 1-accp and 3-accp were present in a 1:4 ratio. subsequently the diacetylated product, 1,3-o-o-diacetyl cp [1,3-(ac)2cp], was enzymatically produce ...1979119483
effects of cerulenin on antibiotic-induced lysis of streptococcus faecalis (s. faecium).addition of the antibiotic cerulenin to cultures lowered the minimal effective concentration of penicillin g or methicillin required to produce bacterial lysis and killing. this effect was most pronounced at subinhibitory antibiotic concentrations. cerulenin had no significant effects on lysis or killing induced in the presence of d-cycloserine, fosfomycin, bacitracin, or vancomycin.1979119485
paper chromatography-enzyme spray technique for the detection of sugar nucleotides with galactose and n-acetyl-galactosamine residues. 1979119791
gut flora of galleria mellonella suppressing ingested bacteria. 1979119813
antagonistic effects of bacillus natto and streptococcus faecalis on growth of candida albicans.the growth-inhibitory effects of bacillus natto and streptococcus faecalis on canida albicans were investigated. when inoculated into the filtrate of a long-term culture of b. natto strain bn (bn), a stock culture of c. albicans rimd 0301020 lost its viability completely, whereas c. albicans rimd 0301011, a fresh isolate from a clinical source, did not. in continuous flow (cf) culture the growth of both strains of c. albicans was suppressed by mixed cultivation with bn. on the other hand, in cla ...1979119897
[association of streptococcus d endocarditis with digestive cancer. critical remarks on the concept of "port of entry"]. 1979119947
experimental endocarditis in rabbits. 3. significance of the proteolytic capacity of the infecting strains of streptococcus faecalis.insertion of a polyethylene catheter into the left ventricle of the heart was used for regular establishment of sterile endocarditis, and bacterial endocarditis was established by injection of approximately 10(8) streptococcus faecalis into the blood stream at the same time as removal of the catheter which had been in place for 3 days. 100 out of 102 rabbits died spontaneously of bacterial endocarditis. evidence is produced that the host-parasite interaction is influenced by the proteolytic prop ...1979120105
the occurrence of candida albicans in lake ontario bathing beaches.there are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. through surveys of four bathing beaches on lake ontario, it was established that the opportunistically pathogenic yeast candida albicans occurs in near shore waters. the beaches surveyed could be differentiated on the basis of bacterial fecal ...1979120220
development of a selective medium for the isolation of clostridium sporogenes and related organisms. 1979120359
[evaluation of the viral contamination of the sea water after the emission of an effluent into the sea (author's transl)].research of virus in the sea water has been made by the concentrated method of adsorption-elution on glass powder. --this method has enabled us to strike the balance on the concentration of virus in the sea water, from the emission to the bank. the frequency of isolation of virus in the bathing zone considered as healthy is very high. the concentration of virus is practically constant in the length of an axis, 200 m distant in comparison with the bank.--the increase of particles of virus in the ...1979120651
[examinations on the behaviour of grampositive and gramnegative bacteria in aqua bidest and tap-water at different initial colony counts and different temperatures (author's transl)].experiments were done about the behaviour of 5 different strains of staph. aureus, strept. faecalis, e. coli, ser. marcescens and p. aeruginosa in aqua bidest and sterile tap water at 20 degrees c, 30 degrees c and 40 degrees c. the behaviour of 3 different initial colony counts in each case was examined. colony forming units (cfu) were evaluated at the beginning and after 1, 2, 4, 6 and 24 h. cfu were reduced at staph. aureus and e. coli in aqua bidest and tap water not earlier than after 4 h, ...1979120654
[crossed resistance to aminoglycoside antibiotics in streptococcus faecalis]. 1979120957
[preliminary note on the study of seawater pollution of the saharan coast of tunisia].the study to an analyse of 726 samples of sea water, taken monthly (1977-1978) at 23 stations. at the basis of the data concerning the npp of total coliformas, 75,5% of the samples point out a clean water for the bathing place, 11,04% = "acceptable" and 9,96% = "dirty". considering the data about the mpp of fecal coliformas and of fecal streptocques, the sanitary state is reported in 94,23% "clean for the bathing place", 1,77% "acceptable" and 4% dirty.1979121049
microbiological assay for antifolate using folic acid assay method. 1979121102
influence of lipids from listeria monocytogenes on the course of infections due to some gram-positive bacteria in mice.the influence of lipids from listeria monocytogenes on the course of infections due to some gram-positive cocci streptococcus pyogenes, streptococcus faecalis and staphylococcus aureus and l. monocytogenes was studied in white mice. varying doses of the lipid preparation were administered before infection. mortality of the animals and rate of elimination of the bacteria from the organs and blood circulation were recorded. in appropriate doses, the lipids markedly enhanced resistance to the afore ...1979121229
disinfection of sewage by ultraviolet irradiation.disinfection of sewage from an activated sludge process, and of sewage from an activated sludge process followed by chemical precipitation, has given very high killing rates for the indicator organisms examined. for coliforms, escherichia coli and faecal streptococci, a killing rate of more than 999.99% was usually obtained, and the residual number of microorganisms in the irradiated sewage was very low. the killing rate increased with the irradiation dosage, depending on the intensity of the ir ...1979121372
[factors influencing the growth and survival in vittel mineral water of various bacteria of intestinal origin and of a strain of pseudomonas fluorescens]. 1979121453
the effect of prophylactic tinidazole on the anaerobic vaginal flora in patients undergoing gynaecological surgery.the effects of prophylactic tinidazole on the vaginal carrier state of anaerobic bacteria and the development of postoperative infections were studied in 50 patients undergoing gynaecological surgery. the results are based on a double-blind trial, using tinidazole (fasigyn; pfizer) and placebo. although no postoperative infections other than urinary tract infections were observed in our series, this study shows conclusively that the isolation of anerobic bacteria from the vagina decreased marked ...1979121639
trout mortalities as a result of streptococcus infection.excessive mortalities were experienced in the bigger rainbow trout (salmo gairdneri) at an efficiently managed trout farm. all tests for known toxins in the feed and water proved to be negative. a faecal streptococcus which belongs to the lancefield group d but which could not be identified as belonging to any of the recognized species within this group, was isolated from the spleens, livers and kidneys of affected fish. pathogenicity studies with this organism proved it to be highly fatal to tr ...1979121769
bacteraemia and upper gastrointestinal fibre-endoscopy. 1979121891
the steady state kinetics of tyrosine decarboxylase from streptococcus faecalis.the present study has explained the general reaction mechanism of the bacterial tyrosine decarboxylase. the rate equation for this mechanism has been presented. the steady state kinetics of tyrosine decarboxylase, as for tyrosine transaminase, have shown that the apoenzyme can bind not only the coenzyme, but also the non-enzymatically formed schiff base between the coenzyme and the substrate. our data then have confirmed the importance of the non-enzymatically formed schiff base in the b6-depend ...1979121930
[clinical applications of sisomicin and netilmicin. previous and current state of microbiological results]. 1979122080
[microbiological quality of frozen vegetable foods. ii. indicator microorganisms]. 1979122280
analysis of plasmid deoxyribonucleic acid in a cariogenic strain of streptococcus faecalis: an approach to identifying genetic determinants on cryptic plasmids.streptococcus faecalis strains nd539 and og1 have been previously shown to be cariogenic in gnotobiotic animals. deoxyribonucleic acid analyses have revealed the presence of a single 26-megadalton plasmid designated pam539 in the former strain, whereas the latter strain was found to be plasmid-free. by gene transfer experiments, it was possible to construct isogenic pairs of strains that differed only with regard to the presence or absence of pam539. comparative studies of isogenic pairs showed ...1977122512
characterization of plasmids determining hemolysin and bacteriocin production in streptococcus faecalis 5952.two plasmids designated pob1 and pob2 were isolated from streptococcus faecalis strain 5952 and found to have molecular weights of approximately 46 x 10(6) and 28 x 10(6), respectively. pob1 was found to determine hemolytic activity and was transmissible, whereas pob2 appeared to determine a bacteriocin that is specifically inhibitory to s. faecalis strains harboring the 26-megadalton plasmid pam539.1977122514
penicillin-netilmicin synergism against streptococcus faecalis.the combination of penicillin plus netilmicin was synergistic in vitro against 28 strains of streptococcus faecalis and compared favorably with penicillin in combination with gentamicin. similarly, penicillin plus netilmicin was as effective as penicillin plus gentamicin in the therapy of 67 rabbits with enterococcal endocarditis produced with a streptomycin-susceptible (s) or a streptomycin-resistant (r) strain of s. faecalis. after 5 days of infection, control rabbits had bacterial titers of 1 ...1978122522
penicillin and netilmicin in treatment of experimental enterococcal endocarditis.successful therapy of enterococcal endocarditis requires the use of a combination of penicillin plus an aminoglycoside. the effectiveness of penicillin (pen), streptomycin (str), and netilmicin (net), a new aminoglycoside, alone and in combination, were studied in vitro and in the treatment of left-sided enterococcal endocarditis in rabbits. in vitro pen+str or net resulted in a more rapid and more complete bactericidal effect than pen, str, or net alone against a str-susceptible strain of enter ...1978122526
solubilization of bacterial membrane proteins using alkyl glucosides and dioctanoyl phosphatidylcholine.the non-ionic detergent octyl glucoside solubilizes a substantial amount of streptococcus faecalis membrane protein without loss of the monitored enzyme activities. a secondary detergent, dioctanoyl phophatidycholine, appears to increase the yield of solubilized material. in addition, the effect of ionic strength indicates that it may be possible to selectively extract groups of membrane proteins by their characteristic solubility at different ionic strengths. the solubilized membrane-associated ...1975123771
interaction of bacterial cell wall polymers and rat macrophages.processing of group a and group d streptococcal cell wall was measured after phagocytosis by normal rat peritoneal cells in tissue culture. group a cell wall was practically non-biodegradable in contrast to group d, which was over 80% degraded by 4-8 days in culture. there was no difference in elimination or degradation of mucopeptide or polysaccharide of group a cell walls. neither antiserum or sensitized lymphocytes affected persistence. macrophages from fisher rats (susceptible to group a cel ...1975126561
the suitability for rats of an intestinal microflora of mice tested under practice circumstances. 1975126608
studies of substructure and tightly bound nucleotide in bacterial membrane atpase.highly purified preparations of streptococcus faecalis atpase contain a similar but inactive protein detected by prolonged polyacrylamide gel electrophoresis. the inactive protein appears to arise by proteolytic cleavage of the major subunits in the enzyme. by use of a new technique, subunit analysis in sds gels was performed on the enzyme band and the inactive protein band excised from a polyacrylamide gel after electrophoresis. the results indicated that the atpase has the composition alpha3be ...1975127088
role of mg2+ ions in the subunit structure and membrane binding properties of bacterial energy transducing atpase. 1976131554
alkyl streptozotocin analogues with improved biological activities.alkyl 16alpha- and -beta-glycosides of a series of n3-alkyl homologues of streptozotocin were synthesized from glucosamine hydrochloride. these compounds, when tested against ascites sarcoma 180, ehrlich ascites carcinoma, or leukemia l1210, exhibited potent antitumor activities, and antibacterial and diabetogenic activities were eliminated. furthermore, the acute toxicities of these compounds were lower than that of streptozotocin. the methyl, ethyl, n-propyl, and n-butyl glycosides of streptoz ...1976133244
chymotryptic conversion of bacterial membrane atpase to an active form with modified alpha chains and defective membrane binding properties. 1976136983
[energization of membrane vesicles from the cells of glycolyzing bacterium streptococcus faecalis].membrane fractions were isolated from streptococcus faecalis cells of a glycolyzing microorganism, devoid of the respiratory chain, using the methods of osmotic shock of the protoplasts, ultrasonic treatment of the cells and ultrasonic treatment of the protoplasts. all fractions possessed the atpase activity, the highest activity being observed in the fraction isolated by ultrasonication of the protoplasts. all preparations were estimated with respect to the presence of vesicles, formed by the " ...1976141304
[enterococcal l-tirosine decarboxylase (author's transl)]. 1977141343
inhibition of streptococcal adenosine triphosphatase by fluoride. 1977142783
[osmotic activity and ionic permeability of membrane vesicles from streptococcus faecalis and micrococcus lysodeikticus cells].membrane fractions containing osmotically active vesicles with sufficiently low membrane permeability for k+, na+ and cl- ions typical for the intact cell membrane were isolated from the cells of the glycolyzing bacterium streptococcus faecalis. in their osmotic properties and ionic permeability the membrane fractions of s. faecalis were found similar to those of the respiring bacterium micrococcus lysodeikticus, which are capable of the energy-dependent potassium transport. it may be thus assum ...1977145881
isolation and characterization of a potassium specific ionophore from streptococcus faecalis.the synthesis of a potassium and rubidium specific ionophore by streptococcus faecalis has been proved, using bilayer measurements. the characteristics of this substance agree with the ones, published for free mobile carriers, such as valinomycin and the macrotetrolides.1977146354
accessibility of the alpha chains in membrane-bound and solubilized bacterial atpase to chymotryptic cleavage. 1978149539
[enterococcal flora of some species of synanthropic flies].the study of enterococcal species present in 16 species of synanthropic flies revealed the predominance of str. faecalis and its variants, a high percentage of fly populations being infected. the spectrum of enterococci was proved to depend on the living conditions of the flies. the percentage of musca domestica with the infected digestive tract was found to vary with changes in the age composition of the fly population.1979155964
chemotherapeutic nitroheterocycles. 18. 2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups.2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups and related compounds (41-69, table ii) were synthesized and their antimicrobial activities were evaluated (table iii). some of these compounds (e.g. 47, 52, and 59) suprisingly exhibited a broad antibacterial spectrum including proteus species and pseudomonas aeruginosa. extraordinary antitrichomonal activities could also be observed in vitro (mic of compound 59, 0.0004 pg/ml) and ...1975166185
[studies to the in-vitro-effectiveness of spectacillin (author's transl)].in this study the sensibility of important disease germs of a clinical and an ambulatory test material has been investigated to the substances "epicillin" and "ampicillin". thereby could be found that 67% (43%) of the e. coli and 55% (36%) of the proteus type, as well as 96% (96%) of the enterococcus type of the ambulatory (clinical) test material were sensitive to epicillin and ampicillin. staphylococcus not producing penicillinase, were well-sensitive, while staphylococcus producing penicillin ...1975166291
[experimental investigations concerning spectrum, serum concentration, and excretion of spectacillin (author's transl)].by means of the diffusion test, the range of action of the ampicillin successive preparation spectacillin was determined using 12 000 pathological germs freshly isolated from in-patients. the result was a range of action similar to that of ampicillin. a comparison, however, is only possible in case the test platelets (without over-dosage) are each charged with exactly, e.g., 25 gamma. the analyses of serum concentrations have shown that spectacillin is satisfactorily absorbed after oral as well ...1975166292
reduced diphosphopyridine nucleotide peroxidase. intermediates formed on reduction of the enzyme with dithionite or reduced diphosphopyridine nucleotide.dpnh peroxidase is a flavin adenine dinucleotide-containing flavoprotein. anaerobic titration of enzyme with dithionite has shown that the active site of the enzyme contains 2 mol of flavin and in addition 1 mol of a non-flavin electron acceptor that is tentatively identified as a disulfide group. thus complete reduction of the enzyme requires 3 mol of dithionite per mole of active site. the first mole of dithionite reduces the non-flavin acceptor; complex formation between the reduced acceptor ...1975166990
survival of human enteric and other sewage microorganisms under simulated deep-sea conditions.the survival of pure cultures of escherichia coli, streptococcus faecalis, clostridium perfringens, and vibrio parahaemolyticus under simulated deep-sea conditions of low temperature (4 c), seawater, and hydrostatic pressures ranging from 1 to 1,000 atm was determined over a period exceeding 300 h. the viability of e. coli and total aerobic bacteria in seawater-diluted raw sewage subjected to these deep-sea conditions was also measured. there was a greater survival of both e. coli and s. faecali ...1975169733
bacterial growth in seafoood on restaurant premises.operational guidelines for a chain of the newer type of seafood restaurants and take-out stores were derived from plots of bacteriological and trimethylamine changes during the preparation and storage of cod fillets, shrimp, and clams. if fish is to be defrosted at room temperature it should be washed and portioned within 14 h. subsequent storage at 5 degrees c should not exceed 72 h and once removed from the refrigerator it should be fried within 3 h. new stores should consider improved methods ...1975172207
bacterial food-poisoning. 1975173111
specificity in enzyme inhibition. 3. synthesis of 5-substituted 2,2-dimethyl-4-imidazolidinones as inhibitors of tyrosine decarboxylase and histidine decarboxylase.2,2-dimethyl-4-imidazolidinone derivatives of the alpha-amino acids dl-phenylglycine (1), dl-phenylalanine (2), l-tyrosine (3), l-histidine (4), and l-tryptophan (5) were prepared in order to assess their specificity in inhibiting amino acid decarboxylases. treatment of th alpha-aminonitriles with acetone in the presence of base and heat or treatment of the alpha-amino amides with acetone gave the title compounds in 48-85% yield. the compounds afforded moderate ability to inhibit the decarboxyla ...1976173851
in vitro bacteriological evaluation of the effectiveness of antimicrobial irrigating solutions.nineteen strains of pathogenic aerobic bacteria were exposed for fifteen seconds in vitro to varying concentrations of five antibiotics and of polyvinyl povidone iodine in saline solution. the presence of human plasma in the solution (30 per cent by volume) did not affect bacterial sensitivity to the antibiotics. most of the bacteria were sensitive to the solutions during that brief exposure. bacteria which were more sensitive by disc sensitivity tests were more sensitive to the solutions. polyv ...1976175069
antibacterial activity of oxidized regenerated cellulose.it has been demonstrated that oxidized regenerated cellulose promptly and markedly reduces the bacterial census of ten different strains of common pathogens when exposed to them in vitro. this was not true of two other hemostatic agents tested, namely, absorbable gelatin sponge and topical thrombin. wounds in guinea pigs in which oxidized regenerated cellulose was placed and infected with one of three pathogens had healing per primum in 39 of 40 instances. in similar control wounds and incisions ...1976176740
[the effect of standardised sodium hypochlorite solution on bacteria pathogenic for the urinary tract (author's transl)].stable sodium hypochlorite solution shows excellent disinfectant properties, even in higher dilution. parallel to clinical tests in vitro experiments were made to show the changes of morphology of bacteria pathogenic for the urinary tract by scanning microscope. two hours after incubation of the bacteria with sodium hypochlorite solution containing 40 mg available chlorine/1 severe damages of the bacterial morphology up to complete destruction were evident in all cases. gram-negative rod-shaped ...1976176759
[bacteriological studies on alimentary egg paste. hygienic and sanitary aspects of the problem]. 1975179481
[hygienic evaluation of the finding of the causative agents of food poisoning in food products]. 1975180687
vancomycin. 1976183412
[researches on the water quality of the river po and its tributaries between cremona and casalmaggiore. i. materials and methods (author's transl)].during 1971 a research program about the status of pollution of the river po and its tributaries was started by six institutes of hygiene altogether (turin, pavia, milan, parma, modena and ferrara universities) with the institute for water research of c.n.r. and hydrographic office of magistracy of the po. the role of the parma university hygiene institute was to study the water qualities of the river po between cremona and casalmaggiore and of two right side tributaries, arda-ongina and taro. t ...1976189776
role of anaerobic bacteria in spontaneous peritonitis of cirrhosis: report of two cases and review of the literature.the role of anaerobic or microaerophillic bacteria in spontaneous peritonitis of cirrhosis has not been clearly defined. among 126 cases recorded in the literature, in only eight (6 per cent), including the two reported here, was bacterascites associated with anaerobic or microaerophilic bacteria. clinical features in these cases were indistinguishable from those associated with aerobic bacteria. however, polymicrobial bacterascites occurred in four of eight cases associated with anaerobes, as c ...1977190883
[effect of polymyxin m on intestinal microorganisms of gnotobiotic chicks].the effect of polymyxin m on the microflora artifically introduced into the digestive tract of gnotobiotic chickens was studied. it was found that polymyxin m caused dysbacteriosis in the digestive tract. reduction of the impaired biocenosis after discontinuation of polymyxin use was rapid and practically complete.1977190946
pivmecillinam in urinary tract infections: a correlation of urinary bactericidal activity with clinical efficacy. 1977193813
bacteriology of the human biliary tract and the duodenum.using the modern anaerobic transport media and meticulous culture techniques, 74 patients undergoing biliary tract surgery were studied. the biliary system was found to be sterile in 58 patients (78%). fifteen patients had 35 isolates of aerobic and facultative bacteria. the most common ones were klebsiella, enterococcus, and escherichia coli. the only anaerobe isolated was clostridium perfringens. eight of 17 patients (47%) with acute cholecystitis and five of 49 patients (10%) with chronic cho ...1977195558
an antiviral subtance extracted from streptococcus faecalis. 1977197425
[viability of enteroviruses in water in the presence of representative bacterial microflora and bacteriophages]. 1977197753
dpnh peroxidase: effector activities of dpn. 1977199166
hemiacetal bond-formation during periodate oxidation of a heteroglycan of d-glucose and d-galactose. 1977199351
anaerobes in human biliary tracts.during a 2-year period, 1,892 patients underwent biliary tract surgery at the mayo clinic. both aerobic and anaerobic cultures of bile were performed in 371 of these patients. sixty-nine percent of the cultures were positive, and 41% (117) of these grew anaerobes, although they were present in pure culture only twice. mixed cultures most commonly contained four different organisms (three aerobes and one anaerobe). bacteroides fragilis was the single most commonly isolated anaerobe and ranked fou ...1977200634
inhibition of protein synthesis in streptococcus faecalis by ochratoxin a.a non-competitive inhibition of binding of camp to bovine protein kinase by ochratoxin a (ota) is shown. preliminary evidence of a protein kinase in streptococcus faecalis is presented. the camp stimulation of this kinase is also inhibited by ota. at the lowest ota concentrations, rna and protein synthesis are inhibited in s. faecalis. the inhibition of rna synthesis is secondary, as in the presence of chloramphenicol no inhibition occurs for 10 min after the addition of ota. the synthesis but n ...1978205331
superoxide dismutase and oxygen metabolism in streptococcus faecalis and comparisons with other organisms.streptococcus faecalis contains a single superoxide dismutase that has been purified to homogeneity with a 55% yield. this enzyme has a molecular weight of 45,000 and is composed of two subunits of equal size. it contains 1.3 atoms of manganese per molecule. its amino acid composition was determined and is compared with that for the superoxide dismutases from escherichia coli, streptococcus mutans, and mycobacterium lepraemurium. when used as an antigen in rabbits, the s. faecalis enzyme elicite ...1978206536
nucleoside 5'-monophosphate analogues. synthesis of 5'-sulfamino-5'-deoxynucleosides.the synthesis of two new nucleotide analogues is described. 5'-sulfamino-5'-deoxyadenosine (1) was prepared by reaction of 5'-amino-5'-deoxyadenosine with (ch3)3n.203, and 5'-sulfamino-5'-deoxythymidine (2) was prepared from 5'-amino-5'-deoxythymidine by a similar reaction. the 5'-sulfamino nucleosides are shown to be quite stable to hydrolysis in acidic or basic aqueous solution tests show that these compounds do not inhibit the growth of escherichia coli or l1210 cells at concentrations less t ...1978209190
monensin, eimeria tenella infection, and effects on the bacterial populations in the ceca of gnotobiotic chickens.bacteria-free chicks in separate plastic film isolators were inoculated orally with single species of bacteria. within an isolator, half the birds were fed unmedicated feed and half received feed containing 100 ppm monensin. with clostridium perfringens as the established species of monoflora, bacterial counts from the duodenum were 10(4) times lower and counts from the ceca were three times lower in monensin-fed birds compared to unmedicated birds. infection with eimeria tenella stimulated an e ...1978209434
antibiotic irrigation and catheter-associated urinary-tract infections.to investigate the efficacy of antibiotic irrigation in preventing catheter-associated urinarytract infection, we carried out a randomized controlled trial of a neomycin-polymyxin irrigant administered through closed urinary catheters. eighteen of 98 (18 per cent) of the patients not given irrigation became infected, as compared with 14 of 89 (16 per cent) of those given irrigation, yielding a mean daily incidence of 5 per cent in each group. the distribution of organisms and their antibiotic se ...1978210379
calcification of selected strains of streptococci. 1979226568
microbial oxidation of protoporhydrinogen, an intermediate in heme and chlorphyll biosynthesis. 1979228599
the antibody-enzyme analogy. comparison of enzymes and antibodies specific for phosphopyridoxyltyrosine.reduced schiff base compounds of pyridoxal-p and tyrosine, which were used to induce specific antibodies described in the preceding article (v. raso and b. d. stolar, biochemistry, 1975), caused active site-directed inhibition of tyrosine transaminase and tyrosine decarboxylase. the antibodies, studied as analogs of enzymes, were able to bind an unsaturated schiff base catalytic intermediate, as shown by equilibrium dialysis and absorbance difference spectroscopy. schiff base formation can proce ...1975234248
transport and metabolism of folates by bacteria.transport of labeled folic acid (pteglu), pteroylpolyglutamates (pteglu3-5), 5-methyl-tetrahydrofolate (5-methyl-h4pteglu), and methotrexate in late-log phase cells of lactobacillus casei was active, and subject to inhibition by unlabeled pteroylmonoglutamates, pteroylpolyglutamates, and iodoacetate, but not glutamate or glutamate dipeptides. pteroylpolyglutamates were transported without prior hydrolysis and shared a common uptake system with pteroylmonoglutamates. the affinity and maximum velo ...1975234963
6-phosphogluconate dehydrogenase from streptococcus faecalis. 1975236442
a reproducible method for reading folate assays with observations on perinatal changes in tissue folate concentration. 1975238032
the inhibition of bacterial growth by ochratoxin a.a series of bacterial species was examined for their sensitivity to ochratoxin a. only grampositive bacteria could be inhibited, generally at a ph lower than 7.0. bacillus subtilis did not show any reduction of growth rates in presence of ochratoxin a, but had a prolonged lag phase. with staphylococcus pyogenes var. aureus and streptococcus faecalis, a prolonged lag phase and a reduction of the growth rate was observed. most sensitive was streptococcus faecalis in the exponential-growth phase. t ...1975238733
purification and properties of an nadp-specific 6-phosphogluconate dehydrogenase from streptococcus faecalis.a procedure is described for the purification of 6-phosphogluconate dehydrogenase (6-phospho-d-gluconate:nadp oxidoreductase (decarboxylating) ec 1.1.1.44) from cell extracts of streptococcus gaecalis. a 180-fold purification was achieved with an over-all yield of about 12% and an average specific activity of 14. the enzyme was homogeneous as determined by polyacrylamide gel electrophoresis, immunoelectrophoresis, and sedimentation equilibrium, studies. its weight average molecular weight, as me ...1975238996
comparative inhibitory activity of bl-s640 and two other cephalosporins.in vitro antibacterial activity of bl-s640 was compared to that of cephalothin and cephalexin against gram-negative and gram-positive bacteria isolated from clinical specimens. bl-s640 demonstrated the best activity on nearly all microbial species studied, except for haemophilus influenzae and diplococcus pneumoniae against which cephalothin was slightly more active.1975239917
studies of introital colonization in women with recurrent urinary infections. i. the role of vaginal ph.the ph of the vaginal introitus was compared to quantitative cultures of the introital mucosa (escherichia coli, proteus mirabilis and streptococcus faecalis) in 800 samples. introital carriage of more than 100 bacteria per ml. was significantly greater at ph of more than 4.4 (p less than 0.005) when compared to mucosal ph of less than or equal to 4.4.1975240038
synthesis and antibacterial properties of methylsulfinyl and methylsulfonyl analongs of some nitrofurans.the sulfoxides 5-methylsulfinyl-2-furaldehyde semicarbazone (2) and 1-[(5-methylsulfinyl-2-fufurylidene)amino]hydantoin (3) as well as the sulfones 1-[(5-methylsulfonyl-2-furfurylidene)animo]hydantoin (1) and 1-(5-methylsulfonly-2-furyl)-2-(6-amino-3-p-ridazyl)ethylene hydrochloride (4) have been prepared and tested for antibacterial activity against a number of gram-negative and gram-positive organisms. the compounds are much less active than the corresponding 5-nitrofuran derivatives, possibly ...1975240940
antimicrobial activity of human peritoneal fluid.cell free peritoneal fluids from 23 normal women had antimicrobial activity which was directed against gram-positive and gram-negative bacteria and also against yeasts. the activity was bacteriostatic or bactericidal and seemed to be due to the presence of a variety of factors which varied in their heat stability and dependence on complement. lysozyme was present in the fluids but was not responsible for all the antimicrobial activity.1975242084
antimicrobial spectrum, pharmacology and therapeutic use of antibiotics. part 2: penicillins.the mechanism of action, resistance, antibacterial spectrum, clinical pharmacology, adverse effects, and therapeutic and prophylactic use of penicillins are reviewed. the choice of a penicillin is discussed. the only indication for the penicillinase-resistant penicillins is the suspected or demonstrated presence of staphylococcus aureus. there are no important differences in therapeutic effect among oxacillin, cloxacillin, dicloxacillin or flucloxacillin by the oral route, or among oxacillin, di ...1977318800
cephalexin: clinical and laboratory evaluation in infants and children.cephalexin appears to be a highly effective antibiotic. it possesses the qualities of rapid oral absorption, production of high drug levels in the blood and urine, and near absence of side effects. it is effective against infections due to gram-positive cocci infections, with the exception of enterococcus, and for most infections caused by e. coli and klebsiella. it is useful in the treatment of a wide variety of infections in infants and children, and particularly valuable with susceptible infe ...1977324691
[quantitative and qualitative bacteriological studies on urine specimens obtained by suprapubic needle aspiration and on midstream-voided samples].in 398 patients with suspected urinary tract infection, quantitative and qualitative bacteriological studies were conducted in urine samples obtained by suprapubic needle aspiration of the bladder (bpu) and in midstream-voided specimens (msu) collected immediately following the aspirations. in msu, bacteria were found in 96.5% of all cases and in bpu in only 38.2%. of the infected msu, 63.3% showed mixed infections, while mixed cultures were found in only 11.2% of infected bpu. in 80% of the inv ...1978351798
[changes in the total microbial count, colibacillus count and enterococcus count in surface water samples kept at various temperatures]. 1978358151
identification and characterization of a small sequence located at two sites on the amplifiable tetracycline resistance plasmid pamalpha1 in streptococcus faecalis.streptococcus faecalis dt-11 harbors the 6.0-megadalton plasmid pamalpha1, which determines resistance to tetracycline (tc). when this strain is grown in the presence of tc for a number of generations, a reversible gene amplification occurs, generating tandem repeats of a 2.65-megadalton segment of the plasmid. on the basis of heteroduplex studies between various forms of pamalpha1 and fragments generated by the escherichia coli restriction endonuclease ecori, we have obtained direct evidence fo ...1977401496
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