| precision of bioassays for apparent and true metabolizable energy adjusted to zero nitrogen balance. | formulae are presented for calculating the variance of estimates of amen and tmen obtained from several commonly used bioassay protocols. formulae are also developed for calculating the variance of the difference between bioavailable energy (be) estimates of two materials tested in the same bioassay. methods are described for determining the allocation of experimental units among dietary treatments that minimizes the variance of amen or tmen estimates in bioassays based upon a completely randomi ... | 1989 | 2704668 |
| comparative virulence of human isolates of coagulase-negative staphylococci tested in an infant mouse weight retardation model. | human infections caused by coagulase-negative staphylococci have steadily increased in numbers and severity. causes may be the use of artificial prostheses, immunocompromising chemotherapy and radiation therapy, and sophisticated surgical techniques, to name a few. although the infectivity of coagulase-negative staphylococci as a group has been well documented for humans, attempts to study the pathogenesis of infections caused by individual species of coagulase-negative staphylococci have been h ... | 1989 | 2715323 |
| antimicrobial resistance in nosocomial isolates of staphylococcus haemolyticus. | staphylococcus haemolyticus is frequently cultured from hospitalized patients and is characterized by resistance to multiple antimicrobial agents. we found that s. haemolyticus represented 70 of 524 (13%) coagulase-negative staphylococcal isolates identified by the clinical microbiology laboratories of two hospitals over 2 months. s. haemolyticus isolates were recovered from wounds (44%), urine (26%), blood (10%), and other sources (20%). all s. haemolyticus isolates were tested for susceptibili ... | 1989 | 2729941 |
| adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine. | a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ... | 1989 | 2770471 |
| [epidemiologic and clinical study of infection caused by hepatitis delta virus in the health service area of la coruna]. | a prospective study on 175 hepatitis hbsag positive patients was carried out between march 1986 and june 1987 in order to study the epidemiological and clinical aspects of the hvd infection. the global prevalence of this infection was 19.42%, with a statistically relevant predominance amongst intravenous drug addicts (ivda). moreover, in most cases it was associated with liver disease (91.17%). chronic liver disease had a greater incidence amongst hvd positive infected patients and presented mor ... | 1989 | 2772347 |
| nucleotide sequences and expression of cdna clones for boar and bull transition protein 1 and its evolutionary conservation in mammals. | during spermatogenesis, the nucleoproteins undergo several dramatic changes as the germinal cells differentiate to produce the mature sperm. with nuclear elongation and condensation, the histones are replaced by basic spermatidal transition proteins, which are themselves subsequently replaced by protamines. we have isolated cdna clones for one of the transition proteins, namely for tp1, of bull and boar. it turned out that tp1 is a small, but very basic protein with 54 amino acids (21% arginine, ... | 1989 | 2777004 |
| opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony. | | 1989 | 2780954 |
| sciatic nerve regeneration in the rat. validity of walking track assessment in the presence of chronic contractures. | the ability of the walking track assessment of sciatic nerve function to correlate with results of a nerve repair has been well documented following acute nerve injury and after recovery of the nerve following nerve repair. the long-term effects of partial denervation upon the lower extremity of the rat include the development of chronic contractures due to residual partial muscle paralysis and imbalance. the present study evaluated the ability of the walking track technique to portray accuratel ... | 1989 | 2796718 |
| [electrophysiological analysis of the interaction of the hypothalamic preoptic area and the nonspecific system of the thalamus in the rabbit]. | | 1989 | 2806656 |
| smoking and other tobacco use. | | 1989 | 2815598 |
| smoking and other tobacco use. | | 1989 | 2815598 |
| inhibition of adrenomedullary catecholamine release by propranolol isomers and clonidine involving mechanisms unrelated to adrenoceptors. | 1 transmural electrical stimulation (10 hz, 40 v, 1 ms for 60s) increased total catecholamine secretion from perfused cat adrenal glands; this response was enhanced by neostigmine and inhibited by mecamylamine, suggesting that release of acetylcholine from splanchnic nerve terminals was stimulating nicotinic receptors and enhancing catecholamine secretion. 2 isoprenaline, (-)-propranolol and (+)-propranolol (10(-7)-10(-5)m) inhibited the electrically-evoked secretory response by 40-70%; similar ... | 1987 | 2827826 |
| activation of vesicular stomatitis virus fusion with cells by pretreatment at low ph. | fusion of vesicular stomatitis virus (vsv) with vero cells was measured after exposure of the virus to low ph under a variety of experimental conditions. the method of relief of fluorescence self-quenching of the probe octadecylrhodamine was used to monitor fusion. incubation of the virus at ph 5.5 prior to binding to cells led to significant enhancement of fusion at the plasma membrane, whereas fusion via the endocytic pathway was inhibited. fusion of ph 5.5-pretreated vsv showed a similar ph t ... | 1988 | 2832405 |
| high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen. | sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ... | 1988 | 2832429 |
| opioids and behavior: genetic aspects. | three animal models, based on genetic differences in endogenous opioid peptides and opioid receptors, are described. obese mice and rats, whose pituitary opioid content is elevated, may be used to investigate eating disorders. recombinant inbred strains of mice, which differ in brain opioid receptors and analgesic responsiveness, can be used for study of opioid- and nonopioid-mediated mechanisms of pain inhibition. individual reactivity to opioids can be examined in c57bl/6 and dba/2 inbred stra ... | 1988 | 2837410 |
| inactivation of lincosaminide antibiotics in staphylococcus. identification of lincosaminide o-nucleotidyltransferases and comparison of the corresponding resistance genes. | resistance to lincomycin by inactivation has been detected in numerous clinical isolates of staphylococcus; in crude extracts of staphylococcus haemolyticus bm4610 and staphylococcus aureus bm4611, inactivation of lincomycin and clindamycin requires the presence of a nucleoside 5'-triphosphate (atp, gtp, ctp, or utp) as nucleotidyl donor and mg2+ as cofactor. the biochemical mechanism of lincosaminide inactivation was elucidated by determination of the structure of inactivated lincomycin and cli ... | 1988 | 2846528 |
| characteristics of coagulase-negative staphylococci that help differentiate these species and other members of the family micrococcaceae. | one hundred reference strains and 1,240 clinical isolates representing 26 species of the family micrococcaceae were used to evaluate the potential of tests for synergistic hemolysis, adherence to glass, pyroglutamyl-beta-naphthylamide hydrolysis, and susceptibility to a set of five antimicrobial agents for differentiating these species and strains within the species. sixty-eight percent of the clinical isolates exhibited synergistic hemolysis; 69% of the clinical staphylococci but none of the mi ... | 1988 | 2846632 |
| influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines. | sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ... | 1988 | 2847397 |
| mechanisms of resistance to chemicals in arthropod parasites of veterinary importance. | this review evaluates the significance, for ectoparasites, of the four resistance mechanisms that have emerged in arthropods as defence against chemicals used for their control. in particular the contrast of the relative importance of each mechanism in each of the major ectoparasitic pests of economic importance (sheep blowflies, mosquitoes and ticks) is discussed. use is made of the experience gained in attempting to overcome these mechanisms to assess the consequences of resistance, and the ch ... | 1985 | 2863888 |
| peroxisomal oxidases and suggestions for the mechanism of action of insulin and other hormones. | | 1985 | 2863924 |
| glutamine synthetase: a marker of an astroglial subpopulation in primary cultures of defined brain areas. | primary cultures from various areas of newborn mouse brain were developed and characterized. enriched astroglial cultures of the cerebral hemispheres, cerebellum, medulla oblongata and olfactory bulbs contained about 80-90% glial fibrillary acidic protein (gfa) immunolabelled cells. these cultures were composed of a majority of flat, 'protoplasmic-like' cells. the aim of this culture model was used: (1) to study glutamine synthetase (gs) activity during in vitro astroglial development; (2) to co ... | 1988 | 2897279 |
| mutants of actinomyces viscosus t14v lacking type 1, type 2, or both types of fimbriae. | mutants of actinomyces viscosus t14v lacking type 1 or type 2 fimbriae or both were selected by their failure to react with rabbit antibodies against either or both fimbrial antigens. immunospecific double labeling with iron dextran and ferritin-conjugated antibodies showed two types of fimbriae on individual cells of the parent organism, a single type on mutant strains with type 1+2- and type 1-2+ fimbriae and no labeled or unlabeled fimbriae on a type 1-2- fimbria-deficient strain. the mutatio ... | 1988 | 2902012 |
| localization of the mdx mutation within the mouse dystrophin gene. | we have mapped human and mouse x chromosome-specific genomic and cdna probes through an interspecies mus musculus/spretus pedigree which contains the mdx mutation. the positions of these markers relative to one another and to the mdx mutation were delineated. using probes corresponding to segments of the human duchenne muscular dystrophy (dmd) gene transcript, the position of a cross-hybridizing mouse equivalent gene (mdmd) was located. in more than 200 animals mapped, three were identified whic ... | 1988 | 2903046 |
| [antiprogestational action of 5 alpha-dihydronorethisterone]. | | 1985 | 2934946 |
| maternal aging as the important etiological factor in human aneuploidy. | | 1985 | 2937399 |
| acetyl-homocysteine-thiolactone-induced increase of superoxide dismutase counteracts the effect of subdiabetogenic doses of streptozocin. | acetyl-homocysteine-thiolactone (cyt) is an organic thio compound that exerts free radical scavenger activity and increases superoxide dismutase (sod) activity. administration of 32 mg cyt/kg body wt/day/30 days in rats increased sod activity in erythrocytes by 126%, and in pancreatic islets by 202%. treatment affected only the cu-zn fraction of the enzyme. transmission electron microscope observations showed that the damage to the pancreatic beta cells induced by single or multiple subdiabetoge ... | 1986 | 2937674 |
| dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility. | in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ... | 1986 | 2942039 |
| differential effects of recombinant human leukocyte interferons on cell surface antigen expression. | human leukocyte (alpha) interferon (ifn-alpha) is composed of a multigene family within which at least eight different species have been expressed in escherichia coli, isolated, and shown to exert a wide range of biological activities on different human target cells. in this study we utilized eight species of ifn-alpha (a, b, c, d, f, i, j, and k) and investigated their respective capabilities to alter the proliferation of a human breast carcinoma cell line (mcf-7). the antigens studied were all ... | 1986 | 2944575 |
| quality control limits for teicoplanin susceptibility tests and confirmation of disk diffusion interpretive criteria. | for monitoring the performance of teicoplanin susceptibility tests, the following quality control limits are recommended: staphylococcus aureus atcc 29213, mic of 0.12 to 0.5 micrograms/ml; enterococcus faecalis atcc 29212, mic of 0.06 to 0.25 micrograms/ml; and s. aureus atcc 25923, zones 15 to 19 mm in diameter (30-micrograms disks). however, some lots of mueller-hinton agar provided unusually large zones of inhibition with both vancomycin and teicoplanin disks, and these lots should be exclud ... | 1987 | 2958498 |
| in-vitro induction of resistance in coagulase-negative staphylococci to vancomycin and teicoplanin. | twenty strains of coagulase-negative staphylococci (18 strains of staphylococcus haemolyticus and two of s. epidermidis) were serially subcultured in broth media containing subinhibitory concentrations (half the mic) of either vancomycin or teicoplanin. the mic of the antibiotic was again measured after five passages in antibiotic-containing broth. the organisms were then subcultured in broth containing antibiotic concentrations half of the new mic value. the experiment was terminated after 25 p ... | 1988 | 2972670 |
| recognition of staphylococcus saprophyticus in urine cultures by screening colonies for production of phosphatase. | phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. phosphatase-negative staphylococci, such as staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as staphylococcus epidermidis, by spot testing colonies on filter paper saturated with 1 n naoh. phosphatase-positive colonies turned pink within seconds, and phosphatase-negative colonies showed no color. none of 55 s. saprophyticus iso ... | 1985 | 2984240 |
| (taa)n within sequences flanking several intrachromosomal variant surface glycoprotein genes in trypanosoma brucei. | in trypanosoma brucei telomeric copies of intrachromosomal variant surface glycoprotein (vsg) genes are produced through a gene conversion mechanism and are expressed if the telomere is or becomes transcriptionally activated. we have analyzed a sequence that occurs 1 to 1.5 kb 5' to several intrachromosomal vsg genes. this flanking sequence has three distinct sections: a 5' section containing 5 to 116 taa or taa-like tandem repeats; a moderately conserved sequence with an alternating gt characte ... | 1985 | 2987874 |
| [hereditary sclerodactyly and syndactyly]. | a 61-year-old man is described with sclerodactyly of the hands and syndactyly of the second and third toes. hereditary sclerodactyly is a rare condition, beginning in early youth with flexion contracture of the fingers. in this patient the skin of the fingers was sclerotic and thickened, and the dorsal skin of the hands was atrophic and dry. the condition did not progress nor did it show signs of raynaud's phenomenon. both feet showed syndactyly of the second and third toes. the family tree sugg ... | 1985 | 2989220 |
| reactivation of latent murine cytomegalovirus from kidney. | cytomegalovirus (cmv) is presumed to cause latent infection, but the sites of infection are incompletely known. we propose that latent murine cytomegalovirus is present in kidney and may be reactivated by explanation. immunosuppressive agents and allogeneic stimuli may enhance this process. balb/c mice were infected 11 to 14 months previously with 10(5) pfu of smith strain murine cytomegalovirus intraperitoneally. kidneys from 15 infected and nine uninfected mice were washed, minced into 1 to 2 ... | 1985 | 3003444 |
| enhancement of hemopoietic recovery by indomethacin after sublethal whole-body gamma irradiation. | the effect of the non-steroid anti-inflammatory drug, indomethacin, a potent prostaglandin synthesis inhibitor, on the recovery of hemopoiesis was investigated in sublethally gamma irradiated mice. treatment with indomethacin after irradiation was found to increase the granulocyte and lymphocyte counts in peripheral blood. furthermore, an increased rate of the restitution of bone marrow cellularity and of the spleen weight was observed. using the method of 125iodo-deoxyuridine uptake in the sple ... | 1986 | 3020886 |
| expression of tumor cell properties in synovial cells in culture. | the tumorigenic properties of human rheumatoid arthritis synovial cells in culture were investigated. the synovial cells developed good colonies and secreted plasminogen activator (pa) and collagenase in the cell cultures, as do hela cells. since pa and progesterone receptor (pgr) are considered to be end products of estradiol action in breast cancer cells, the estrogen receptor (er) and pgr content in these cells was also assayed. large amounts of er and pgr were detected in the synovial cells ... | 1987 | 3028023 |
| hirano body filaments contain actin and actin-associated proteins. | hirano bodies are eosinophilic, rod-shaped intraneuronal inclusions whose frequency increases with age and with alzheimer's disease. to investigate their composition and possible relationship to the neuronal cytoskeleton, we employed immunocytochemistry and immunoelectronmicroscopy by using antisera to cytoskeletal proteins. the presence of actin, alpha-actinin, vinculin and tropomyosin was demonstrated diffusely throughout the hirano body. the presence of these proteins supports the contention ... | 1987 | 3029338 |
| ligand- and weak base-induced redistribution of asialoglycoprotein receptors in hepatoma cells. | the receptor for asialoglycoproteins (asgpr) was localized in human hepatoma hep g2 cells by means of quantitative immunoelectron microscopy. without ligand added to the culture medium, we found 34% of the total cellular receptors on the plasma membrane, 37% in compartment of uncoupling receptor and ligand (curl), and 21% in a trans-golgi reticulum (tgr) that was defined by the presence of albumin after immuno-double labeling. a small percent of the asgpr was associated with coated pits, the gol ... | 1987 | 3034918 |
| comparison of four serological tests for the detection of specific immunoglobulin m in cord sera of infants congenitally infected with cytomegalovirus. | four serological tests (3 immunoassays using enzyme-labelled antigen and 1 radioimmunoassay) were compared as regards the detection of cytomegalovirus (cmv) immunoglobulin m in cord sera. 68 cord sera from infants congenitally infected by cmv were included in the study. the infections were primarily diagnosed by virus isolation close to birth. four laboratories in 3 countries were involved, each laboratory using its own or a commercial test. of the sera tested in the different laboratories 50-80 ... | 1987 | 3039652 |
| the establishment and characterization of mouse l-929 cells in protein-free eagle's minimal essential medium. | the mouse cell line l-929 was established in protein-free eagle's minimal essential medium. the cells have been 'adapted' to continuous growth in the medium using stepwise reductions in the concentration of fetal bovine serum. the cells designated l-929-ws have now been propagated in protein-free eagle's minimal essential medium for two years. the population-doubling time was about 37 h. the addition of serum stimulated cell growth only slightly, but the saturation density was significantly incr ... | 1988 | 3058707 |
| quality analysis of dsa equipment. | in the framework of a quality analysis project for the improvement of digital subtraction angiography (dsa) equipment, an inventory was made of the image quality and radiation dose of dsa equipments in six hospitals in the netherlands. the image quality was investigated with a contrast detail (cd) phantom. the entrance dose of the radiation on this phantom and the skin dose at the level of the eye lenses and the thyroid gland were measured in these hospitals using a human phantom during a standa ... | 1988 | 3067118 |
| the bacteria responsible for ureolysis in artificial dental plaque. | the origin of ureolytic activity in artificial-mouth plaques was established by assessing the contribution to plaque ureolytic activity of the isolated bacteria. to overcome losses of ureolytic activity caused by the unstable presence of urease in oral bacteria, ureolytic bacteria were isolated from an exceptionally active plaque (1 mumol nh3/min per mg protein) in which 63 per cent of the flora was ureolytic. after their ability to metabolize urea was stabilized, 13 ureolytic bacteria remained: ... | 1988 | 3075450 |
| mechanisms of lymphocyte adhesion to human vascular endothelial cells in culture. t lymphocyte adhesion to endothelial cells through endothelial hla-dr antigens induced by gamma interferon. | the effects of interferons (ifns) on lymphocyte adhesion to cultured human vascular endothelial cells (ec) were investigated using an in vitro assay. endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (cm) from 12-o-tetra decanoylphorbol 13-acetate-concanavalin a (tpa-con a) stimulated human peripheral blood lymphocytes (pbl), or with recombinant (r) gamma interferon (ifn-gamma) or r alpha interferon (ifn-alpha), and then were incubated ... | 1986 | 3084562 |
| air-1, a newly found locus on mouse chromosome 16 encoding a trans-acting activator factor for mhc class ii gene expression. | rj 2.2.5 is a human b cell line that has lost the capacity to express mhc class ii genes. the human class ii-positive phenotype is restored in somatic cell hybrids between rj 2.2.5 and mouse spleen cells. by karyotype and molecular studies of an informative family of hybrids we have now shown that the reexpression of human class ii gene products, as well as the maintenance of the mouse class ii-positive phenotype, correlates with the presence of mouse chromosome 16. thus, the existence on this m ... | 1986 | 3088202 |
| molecular characterization of ro(ssa) and la(ssb) proteins. | ro(ssa) and la(ssb) proteins were characterized using human spleen extract as antigen and monospecific anti-ro(ssa) and anti-la(ssb) sera. the antigens were partially purified by deae cellulose and sephacryl chromatography, and then were electrotransferred to nitrocellulose sheets after sds-page electrophoresis. the anti-ro(ssa) sera had reacted with a polypeptidic band of 58 kilodaltons (kda), meanwhile the la(ssb) antisera reacted with 2 polypeptidic bands of 42 and 40 kda. the molecular weigh ... | 1986 | 3088274 |
| effect(s) of lipopolysaccharide on lectin-induced t-cell activation. | the present study focuses on the effect of lipopolysaccharide (lps) on the cellular events leading to t-cell activation by concanavalin a (con a). interleukin 2 (il-2) production is much reduced in con a-stimulated cultures of spleen cells derived from lps-treated mice. this depressed il-2 synthesis is not related to the eventual activity of lps-activated suppressive b cells. rather, it reflects an ineffective collaboration between adherent cells and t lymphocytes. the low level of il-2 produced ... | 1986 | 3091272 |
| nucleotide sequence of gene lina encoding resistance to lincosamides in staphylococcus haemolyticus. | we have determined the nucleotide sequence of gene lina in plasmid pip855, which confers resistance to lincomycin by inactivating it in staphylococcus haemolyticus. the gene was defined by start and stop codons and an open reading frame of 483 bp corresponding to a product with an mr of 19020. the apparent size of the resistance-conferring protein was 21 kda, when a minicell extract was electrophoresed in the sodium dodecyl sulfate-polyacrylamide gels. full expression of lina was obtained both i ... | 1986 | 3091456 |
| human choriogonadotropin-like material in bacteria of different species: electron microscopy and immunocytochemical studies with monoclonal and polyclonal antibodies. | immunocytochemical studies using antisera to whole human choriogonadotropin (hcg), to its alpha- and beta-subunits and to the cooh-terminal peptide of hcg beta, and two monoclonal antibodies to hcg beta, demonstrated expression of hcg-like material, its individual subunits and/or fragments in nine bacterial strains. seven of these were isolated from patients with cancer and were definitely identified as streptococcus faecalis (three strains), staphylococcus haemolyticus (two strains) and staphyl ... | 1987 | 3116165 |
| [primary leiomyosarcoma of the pulmonary artery. apropos of a case]. | a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ... | 1987 | 3122693 |
| in vitro analysis of t-cell-mediated immunity to intact eggs in murine experimental schistosoma japonicum infection. | to characterize the mechanisms of induction and regulation of the cell population involved in granuloma formation around eggs of schistosoma japonicum, we utilized a simple method of in vitro experiments. lyt1+2-t cells were essential for in vitro responses to the intact s. japonicum eggs, which were assumed to be comparable to in vivo granulomatous responses. t-cell responses seemed to be macrophage-dependent, and responding t cells produced il-2-like activities in the culture supernatant. sera ... | 1987 | 3127618 |
| site of action of a gonococcal growth inhibitor produced by staphylococcus haemolyticus. | the inhibitory substance produced by staphylococcus haemolyticus strain no. 7 acts on growing as well as resting gonococcal cells, as shown by reductions in viable counts. the optical density of these cell suspensions was only slightly reduced. the inhibitor caused lysis of gonococcal spheroplasts at 24 degrees c and 37 degrees c, but was much less active at 4 degrees c. acting on intact gonococcal cells, the inhibitor caused a temperature-dependent release of radioactive cytoplasmic material. e ... | 1988 | 3134553 |
| the amino acid sequence of a gonococcal growth inhibitor from staphylococcus haemolyticus. | a gonococcal inhibitor produced by staphylococcus haemolyticus was separated into three components by reverse-phase h.p.l.c. the amino acid composition analysis of each of the three components indicated extensive similarities. n-terminal sequence analysis of all three components allowed the identification of the first 27-30 residues of each. the complete primary structure of each component was determined from the sequence analysis of trypic peptides and peptides generated by mild acid hydrolysis ... | 1988 | 3138972 |
| rat catcher's warfarin treatment associated with rectal haemorrhage. | | 1988 | 3147777 |
| detection of parasite antigens in different biological fluids. | | 1988 | 3151006 |
| transferrin receptor and 4f2 expression by nk-sensitive and nk-resistant tumour cell lines. | upon stimulation with a phorbol ester and known tumour promoter, 12-o-tetradecanoylphorbol-13-acetate (tpa), the human erythroleukemia k562 cell line, a standard target for human natural killer (nk) cells, shows a significant reduction in expression of transferrin receptors (tfr) and becomes resistant to nk-mediated cytolysis. prompted by the initial finding that expression of tfrs by k562 cells correlates with target sensitivity to nk cytotoxicity, we have investigated the role of two proposed ... | 1988 | 3165309 |
| trisomy 11 in nonlymphocytic neoplasia. | | 1988 | 3165669 |
| suppression of the radiation-induced expression of a tumor-associated antigen in human cell hybrids by the protease inhibitor antipain. | the effect of the protease inhibitor antipain (1.25 micrograms/ml) on the radiation-induced expression of a tumor-associated antigen in human cell hybrids has been investigated. a variety of treatment protocols have been studied where antipain was present before, during and at various times post-irradiation. it was found that antipain suppressed the radiation-induced expression of the tumor-associated antigen in all treatment protocols. the most effective suppression was obtained in those protoc ... | 1988 | 3191580 |
| [echocardiographic assessment of the functional state of an experimental aorto-left ventricular conduit]. | | 1988 | 3209092 |
| immunostimulating staphylococcal lipoteichoic acid prevents pulmonary tumor colonization in balb/c-mice. | immunostimulating and antineoplastic activities of staphylococcal lipoteichoic acid (lta) were studied in balb/c-mice. systemic administration of lta (1 mg or 2 mg i. p., 7 and 4 days prior to challenge) significantly enhanced chemiluminescence response of peritoneal macrophages (p less than 0.0125) and induced enlargement of the spleen (p less than 0.025) as compared to non-treated controls. in vivo the number of lung colonies was significantly lower (p less than 0.0125) in lta-treated mice 14 ... | 1988 | 3223138 |
| [kidney morphology in hemolytic-uremic syndrome]. | morphology of the kidneys in hemolytic-uremic syndrome is considered basing on autopsy findings obtained for 3 infants with 5-17-day history of acute renal failure. a newborn infant of 17 days developed the disease after feto-fetal hemotransfusion when macerated fetus-donor hemolysis products entered the circulation of the fetus-recipient through monochorionic placenta. the second case in an infant of 6 months was due to adtp vaccine. the last infant aged 16 months manifested the syndrome in the ... | 1988 | 3233069 |
| periodicity coding in the inferior colliculus of the cat. ii. topographical organization. | 1. the topographical distributions of single-unit and multiple-unit responses to amplitude-modulated tones--and to other relevant parameters of simple tonal stimuli--were defined across the main frequency representational gradient and within narrow frequency ranges represented in "frequency band laminae" in the principal midbrain auditory nucleus, the central nucleus of the inferior colliculus (icc), in adult, barbiturate-anesthetized cats. 2. responses to amplitude-modulated tones with the carr ... | 1988 | 3236053 |
| periodicity coding in the inferior colliculus of the cat. ii. topographical organization. | 1. the topographical distributions of single-unit and multiple-unit responses to amplitude-modulated tones--and to other relevant parameters of simple tonal stimuli--were defined across the main frequency representational gradient and within narrow frequency ranges represented in "frequency band laminae" in the principal midbrain auditory nucleus, the central nucleus of the inferior colliculus (icc), in adult, barbiturate-anesthetized cats. 2. responses to amplitude-modulated tones with the carr ... | 1988 | 3236053 |
| staphylococcus haemolyticus: cocarde growth around imipenem disks. | twelve multiple-drug-resistant isolates of coagulase-negative staphylococci from diverse clinical sources, which had yielded cocarde growths (double zones of inhibition) around 10 micrograms imipenem disks, were identified as staphylococcus haemolyticus. this was the only staphylococcal species which exhibited this unusual phenotypic trait at our institution during a 7-month observation period. | 1988 | 3243092 |
| studies on type ii progesterone receptor in mcf-7 cells. | these experiments demonstrate for the first time the existence of a type ii progesterone receptor (rpii) in mcf-7 human breast cancer cells. rpii was shown to have a lower affinity for tritiated progesterone ([3h]pg) (kd greater than or equal to 13 nm) than classical rp (kd less than or equal to 3 nm). rpii was detected by cytosolic, nuclear, and whole cell assays of mcf-7 cells. scatchard analysis of [3h]pg binding data revealed that classical rp but not rpii could be recompartmentalized from t ... | 1988 | 3254626 |
| functionally polarized layers formed by epidermal cells on a permeable transparent collagen film. | rat epidermal cells were cultured on a transparent collagen film, which was permeable to low mr substances. then the cells were bathed in both media (apical and basal), which were separated by the collagen membrane. the cells formed a multi-layered epidermal sheet with well-developed structures of desmosomes. this sheet on a permeable support was found to be an effective permeability barrier for glucose and amino acids. the epidermal layer showed functional polarity for the uptake and excretion ... | 1988 | 3255753 |
| maintenance of multiplication rate stability by cell populations in the face of heterogeneity among individual cells. | we addressed the question of how a population of cells can maintain a constant multiplication rate given the heterogeneity of its individual members. using the nih 3t3 line of mouse embryo cells, comparisons were made of multiplication rates between offspring subclones and the parental clones from which the subclones were derived. parents at all levels of multiplication rate produced offspring with a wide range of multiplication rates. offspring from parent cells with the highest growth rates ra ... | 1988 | 3255757 |
| staphylococcus haemolyticus urinary tract infection in a male patient. | urinary tract infections caused by staphylococci are usually attributed to staphylococcus epidermidis or s. saprophyticus. the case study reported here describes a persistent urinary tract infection caused by s. haemolyticus in a 38-year-old male whose infection was ultimately resolved through the use of the antibiotic trimethoprim-sulfamethoxazole. | 1988 | 3260240 |
| human interleukin-2 anti-idiotypes. | we describe the generation of a monoclonal anti-idiotypic (anti-id) antibody directed against affinity purified rabbit antibodies (id) to recombinant human il-2. this monoclonal antibody, 6a12, has the ability to inhibit the neutralization of il-2 activity by the id, suggesting that it may bind at or near the il-2 neutralizing site on the id. 6a12 exhibits il-2 agonist activity on pha-activated human t cells. the purified igg of 6a12 is also shown to bind to a purified soluble recombinant p55 su ... | 1988 | 3264260 |
| synthetic peptides of shiga toxin b subunit induce antibodies which neutralize its biological activity. | shiga toxin b chain, the binding subunit of shiga toxin, was recently purified; and the amino acid sequence of this 7,716-dalton polypeptide was determined (n.g. seidah, a. donohue-rolfe, c. lazure, f. auclair, g. t. keusch, and m. chretien, j. biol. chem. 261:13928-13931, 1986). in the present study, synthetic peptides corresponding to three overlapping sequences from the n-terminal region of this subunit were prepared. the peptides synthesized consisted of residues 5 to 18, 13 to 26, and 7 to ... | 1988 | 3286503 |
| synthetic peptides of shiga toxin b subunit induce antibodies which neutralize its biological activity. | shiga toxin b chain, the binding subunit of shiga toxin, was recently purified; and the amino acid sequence of this 7,716-dalton polypeptide was determined (n.g. seidah, a. donohue-rolfe, c. lazure, f. auclair, g. t. keusch, and m. chretien, j. biol. chem. 261:13928-13931, 1986). in the present study, synthetic peptides corresponding to three overlapping sequences from the n-terminal region of this subunit were prepared. the peptides synthesized consisted of residues 5 to 18, 13 to 26, and 7 to ... | 1988 | 3286503 |
| kingella kingae infection in healthy children. | kingella kingae is a gram-negative occasional, but normal, inhabitant of the nasopharynx. we present two new cases of this infection that occurred in previously healthy children, and compare and contrast them to other cases reported in the literature. k. kingae osteomyelitis generally has an insidious, subacute onset, whereas septic arthritis has an acute presentation. to date, all strains of k. kingae have been sensitive to penicillin, and no residual damage has been reported following osteomye ... | 1988 | 3292580 |
| temporal requirement for epidermal growth factor and insulin in the stimulation of hepatocyte dna synthesis. | primary monolayer cultures of adult rat hepatocytes were used to study the temporal interaction of epidermal growth factor (egf) and insulin in their stimulation of dna synthesis. the hepatocytes were cultured both under defined conditions and with serum. egf and insulin interacted synergistically. the entry into s phase (g1 exit) followed first-order kinetics both in untreated and hormone-stimulated cells. addition of egf and insulin at the time of plating did not alter the lag period before th ... | 1987 | 3294862 |
| campylobacter pyloridis: a new organism to explain an old problem? | | 1987 | 3309916 |
| [medico-genetic studies of human provisional organs]. | | 1987 | 3318529 |
| effect of cyclooxygenase blockade on gas exchange and hemodynamics in pseudomonas pneumonia. | acute bilateral pseudomonas aeruginosa pneumonia was induced in 10 anesthetized dogs, after which five dogs received intravenous indomethacin (2 mg/kg) (indomethacin group), whereas five others were infused with saline (2 ml/kg) (control group). plasma levels of 6-ketoprostaglandin f1 alpha(6-keto-pgf1 alpha) and thromboxane b2 (txb2), stable metabolites of prostacyclin (pgi2) and thromboxane a2 (txa2), respectively, were measured by radioimmunoassay. although txb2 levels were not different befo ... | 1987 | 3320011 |
| toxic effects of natural saline waters on mallard ducklings. | water from 10 saline wetlands in saskatchewan was provided as drinking water for 1-day-old mallards (anas platyrhynchos). ducklings given water with conductivity from 3,750 to 7,490 microns hos/cm grew as well as birds on fresh water during a 14-day trial, but birds given water with conductivity of 4,000 microns hos/cm grew poorly during the last 2 wk of a 28-day trial. ducklings given water with conductivity of 7,720 micron hos/cm grew poorly during a 14-day trial. six of 10 ducklings given wat ... | 1988 | 3352095 |
| protective effect of glucan against experimentally induced staphylococcal mastitis in ewes. | glucan, an immunostimulant, was evaluated for its ability to modify a staphylococcal mammary challenge in ewes. glucan was administered subcutaneously to ewes prior to lactation or during lactation, and all ewes, including a control group, were subsequently challenged intramammarily with staphylococcus haemolyticus 40 days after the mean lambing date. the glucan treatment was shown to modify the staphylococcal mammary infection as the milk bacterial counts from all of the glucan-treated groups w ... | 1988 | 3354193 |
| piezoelectric polymer curvature sensor for measurement of regional curvature radius of lv wall. | to evaluate regional myocardial function, we developed a curvature sensor for direct and instantaneous measurement of the regional curvature radius of the lv wall. the sensor is a bimorph of two sheets of thin piezoelectric polymer film. the relation between output voltage of the sensor and the reciprocal of the known curvature radius has been shown to be linear. in anesthetized dogs, we inserted the curvature sensor into the subepimyocardium with a specially designed introducer. special care wa ... | 1988 | 3364584 |
| effects of estradiol on cerebral cortical neurons and their responses to adenosine. | the effects of iontophoretically applied 17 alpha- and 17 beta-estradiol on the spontaneous firing of, and actions of purines (adenosine, adenosine 5'-n-ethylcarboxamide) on, neurons in the rat cerebral cortex have been determined. both steroids, applied as acetate, sulphate or hemisuccinate esters, depressed the spontaneous firing of approximately half of the neurons tested. 17 beta-estradiol potentiated the inhibitory actions of adenosine on some neurons (26%) and antagonized the effects of ad ... | 1988 | 3370499 |
| subacute bacterial endocarditis in a patient with ankylosing spondylitis. | a 57 year old white man with aortic insufficiency and previously undiagnosed ankylosing spondylitis, who developed subacute bacterial endocarditis (sbe), is described. emergency aortic valve replacement was necessary, and the aortic valve pathology showed diffusely fibrosed and thickened valve leaflets with bacterial vegetations. this is the first recorded case of sbe in ankylosing spondylitis. | 1988 | 3389930 |
| new orally active alpha-ketohydroxy pyridine chelators for the treatment of iron overload. | | 1988 | 3390580 |
| influence of coenzyme q10 on doxorubicin uptake and metabolism by mouse myocardial cells in culture. | | 1988 | 3416371 |
| genetic characterization of leishmania isolates at 37 enzyme loci. | | 1988 | 3417374 |
| experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination. | the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ... | 1988 | 3421507 |
| [topical organization of the neuronal projections of the substantia nigra and ventral field of the tectum mesencephali onto the head of the caudate nucleus in the cat brain]. | the investigation performed on cats by means of retrograde axonal transport of horseradish peroxidase and luminophores has presented the data demonstrating spatial organization of separate part projections of the nigral complex and the tegmental ventral field to various segments of the caudate nucleus head. terminal fields from neurons of various parts of the substantia nigra and the tegmental ventral field are demonstrated to overlap in segments of the caudate nucleus. experiments with double f ... | 1987 | 3426407 |
| experimental study of autologous free omental grafts. | the fate of intraabdominal free omental grafts was studied in 15 dogs and it was found that a connection between the hollow organ and the transplanted omentum was established only when the former had been injured. the larger, pigeon-egg-sized free fatty omental grafts became necrotic already from the fourth day onwards being replaced by an abscess-like structure, while in thin membrane-like grafts tissue necrosis due to the creeping in of connective tissue was less marked. in both cases, an inta ... | 1987 | 3434086 |
| molecular epidemiology of trimethoprim resistance among coagulase-negative staphylococci. | a 42% (70 of 167 isolates) incidence of resistance to 20 micrograms of trimethoprim per ml was found among clinical isolates of coagulase-negative staphylococci from two hospitals. a specific trimethoprim resistance gene probe from a conjugative staphylococcus aereus plasmid was used to investigate the location of the trimethoprim resistance gene among 29 isolates. in 14 trimethoprim-resistant isolates, the probe hybridized with only chromosomal dna, in 9 it hybridized with only plasmid dna, and ... | 1987 | 3435115 |
| phenotypic expression and genetic heterogeneity of lincosamide inactivation in staphylococcus spp. | we examined the resistance phenotype and the genetic basis of lincosamide modification in 25 clinical isolates of staphylococcus spp. inactivating lincomycin and clindamycin. the strains were resistant to high levels of lincomycin but remained susceptible to clindamycin. however, mbcs and inoculum effects showed that the activity of clindamycin was impaired. the distribution in these strains of nucleotide sequences related to lina and lina', the genes encoding lincosamide nucleotidylation in sta ... | 1987 | 3439797 |
| effect of forphenicinol on the production of ia-positive macrophages in mice with or without l1210 leukemia and on the growth of l1210 in immunized mice. | oral administration of forphenicinol, s-2-(3-hydroxy-4-hydroxymethylphenyl)glycine, increased the production of ia-positive peritoneal macrophages in healthy mice. moreover, forphenicinol increased ia-positive macrophages in l1210-bearing mice. though forphenicinol was ineffective against mouse leukemia l1210 when administered alone, in combination with l1210 vaccine it prolonged the survival time of l1210-bearing mice and the increase in the number of ia-positive macrophages in peritoneal cavit ... | 1986 | 3458691 |
| in vitro antistaphylococcal activity of pefloxacin alone and in combination with other antistaphylococcal drugs. | mics of pefloxacin and nine antistaphylococcal drugs were determined for 200 isolates of staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, staphylococcus hominis, and staphylococcus saprophyticus. all the strains were susceptible to pefloxacin, vancomycin, and rifampin. oxacillin-resistant strains were uniformly resistant to cephalothin and were more likely to be resistant to gentamicin, erythromycin, clindamycin, doxycycline, and trimethoprim-sulfamethoxazole than ... | 1987 | 3481244 |
| biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro. | osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ... | 1987 | 3502681 |
| biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro. | osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ... | 1987 | 3502681 |
| fluorescent low density lipoprotein and acetylated low density lipoprotein labeling of cultured bovine trabecular endothelial cells. | | 1987 | 3508231 |
| etiopathogenesis of uric acid and ammonium urate uroliths in non-dalmatian dogs. | the etiopathogenesis of uric acid, sodium acid urate, and ammonium acid urate uroliths in non-dalmatian dogs appears to be a complex phenomenon. it may involve one or more pathologic and/or physiologic processes acting independently or in concert to increase urinary concentration of lithogenic substances that result in initiation, growth, and retention of urate uroliths. increased urine uric acid concentration and/or urinary excretion of uric acid appear to be primary predisposing factors in ura ... | 1986 | 3518203 |
| status of basic external human fertilization. | | 1986 | 3529121 |
| pervasive developmental disorders and immunological tolerance. | a wide range of studies in man and other species suggest that early compromise of immunological tolerance (both maternal-fetal and self) may lead to severe and varied cognitive deficits. this article briefly reviews what is known of the genesis and maintenance of normal tolerance and current ideas on pathological deviances in tolerance. these ideas are discussed in relation to risk factor, family, twin, biochemical, anatomical, and immunological studies of pervasive developmental disorders (part ... | 1986 | 3532100 |
| in vitro susceptibilities of four species of coagulase-negative staphylococci. | the in vitro susceptibilities of 260 strains of coagulase-negative staphylococci to penicillin g, oxacillin, nafcillin, methicillin, cephalothin, and seven non-beta-lactam antimicrobial agents were determined and compared with the susceptibilities of 54 strains of staphylococcus aureus with known patterns of susceptibility. penicillin g susceptibility for s. aureus, staphylococcus epidermidis, staphylococcus haemolyticus, and staphylococcus hominis was readily determined by using beta-lactamase ... | 1986 | 3539009 |
| genotoxicity studies with two antichagasic drugs. | | 1987 | 3553925 |
| suppression of autoimmunity to rat male accessory glands: possible relevance to tumor-host responsiveness. | | 1987 | 3592057 |
| polyunsaturated fatty acids augment free radical generation in tumor cells in vitro. | polyunsaturated fatty acids (pufas) have been shown to inhibit both normal and tumor cell growth in vitro. as pufas are known to induce a respiratory burst and free radical generation in polymorphonuclear leukocytes and since free radicals are toxic to cells, we investigated the effect of pufas on a measure of free radical generation (nitroblue tetrazolium reduction) in normal human fibroblasts and breast cancer cells in vitro. results suggested that linoleate (la), gamma-linolenate (gla), arach ... | 1987 | 3593336 |