| the in vivo association of manganese with the chromosome of micrococcus radiodurans. | | 1976 | 178008 |
| comparative specificity of geranylgeranyl pyrophosphate synthetase of micrococcus lysodeikticus and pumpkin. | comparative studies on the substrate specificity of geranylgeranyl pyrophosphate synthetase from micrococcus lysodeikticus and from pumpkin seedlin revealed that geranyl pyrophosphate was the most active of the natural substrates for the pumpkin enzyme, whereas it was the least active for the bacterial enzyme. a marked difference was also observed between the enzymes from these two sources as regards the reactivity of 3-methyl-2-alkenyl pyrophosphates as a function of the size of the alkyl group ... | 1975 | 178644 |
| change in the ratio of cytochrome oxidase activity to nitrite reductase activity of pseudomonas aeruginosa nitrite reductase with the kind of c-type cytochrome used as an electron donor. | the ratio between the nitrite reductase and cytochrome oxidase activities of pseudomonas aeruginosa nitrite reductase [ec 1.9.3.2.] varies with kind of c-type cytochrome used as the electron donor. withe cytochrome c-548, 554 (micrococcus sp.), the nitrite reductase activity is greater than the cytochrome oxidase activity, while the former is smaller than the latter with cytochrome c-554 (navicula pelliculosa). the aerobic oxidation catalyzed by this enzyme of denitrifying bacterial ferrocytoch ... | 1976 | 178646 |
| oxidative phosphorylation in micrococcus denitrificans: calculation of the p/o ratio in growing cells. | p/o ratios were measured in membrane particles obtained from cells of micrococcus denitrificans, while growing on different carbon sources. the membrane particles obtained from cells growing actively on glucose, succinate, ethanol and propanol as the carbon and energy sources catalyzed oxidative phosphorylation and yielded respective p/o ratios of 1.4, 1.2, 0.8, and 0.5 with nadh, and 0.8, 0.6, 0.6, and 0.5 with succinate as the electron donors. not such a difference in p/o ratio is observed in ... | 1976 | 179486 |
| studies by electron-paramagnetic-resonance spectroscopy on the mechanism of action of xanthine dehydrogenase from veillonella alcalescens. | e.p.r- (electron-paramagnetic-resonance) spectroscopy was used to compare chemical environment and reactivity of molybdenum, flavin and iron-sulphur centres in the enzyme xanthine dehydrogenase from veillonella alcalescens (micrococcus lactilyticus) with those of the corresponding centres in milk xanthine oxidase. the dehydrogenase is frequently contaminated with small but variable amounts of a species resistant to oxidation and giving a new molybdenum (v) e.p.r. signal, "resting i". there is al ... | 1976 | 179532 |
| peptidoglycan biosynthesis in micrococcus luteus (sodonensis): transglycosidase and phosphodiesterase activities in membrane preparations. | two enzyme activities involved in the biosynthesis of peptidoglycan in micrococcus luteus (sodonensis), a transglycosidase and a phosphodiesterase, have been demonstrated in isolated membrane preparations. the transglycosidase activity promotes the in vitro synthesis of an uncross-bridged peptidoglycan that is completely susceptible to lysozyme. this in vitro-synthesized peptidoglycan consists of 76% "soluble" and 24% "insoluble" material. the soluble peptidoglycan is primarily a single low-mole ... | 1976 | 179977 |
| the structure of paracoccus denitrificans cytochrome c550. | the crystal structure of paracoccus (formerly micrococcus) denitrificans cytochrome c550 has been solved by x-ray diffraction to a resolution of 2.45 a. in both amino acid sequence and molecular structure it is evolutionarily homologous with mitochondrial cytochrome c from eukaryotes and photosynthetic cytochrome c2 from purple non-sulfur bacteria. all of these cytochromes c have the same basic folding pattern, with surface insertions of extra amino acids in c550. various strains of c2 have all, ... | 1976 | 180013 |
| classification of micrococci on the basis of deoxyribonucleic acid homology. | the dna homology relationships of 25 micrococci (15 strains of micrococcus, eight strains of sarcina and two strains of staphylococcus) were studied by the deoxyribonucleic acid hybridization method using nuclease s1, an endonuclease specific for single-stranded dna molecules. nineteen of the strains were classified into three groups. group i contained micrococcus lysodeikticus iami056, m. luteus iami1010, m. flavus iami2005 and iami2006, sarcina flava iami2007 and iami1006. s. subflava iami2009 ... | 1976 | 180238 |
| pathway of thiamine pyrophosphate synthesis in micrococcus denitrificans. | the pathway of thiamine pyrophosphate (tpp) biosynthesis, which is formed either from exogeneously added thiamine or from the pyrimidine and thiazole moieties of thiamine, in micrococcus denitrificans was investigated. the following indirect evidence shows that thiamine pyrophosphokinase (ec 2.7.6.2) catalyzes the synthesis of tpp from thiamine: (i) [35s]thiamine incubated with cells of this microorganism was detected in the form of [35s]thiamine; (ii) thiamine gave a much faster rate of tpp syn ... | 1976 | 181359 |
| biological values of the infant, juvenile, and adult agouti (dasyprocta sp) with emphasis on microbial flora. | normal values for intestinal flora were determined on four adult, two juvenile, and four infant agoutis (dasyprocta sp) maintained at our institution. serologic, hematologic, biochemical, and histologic observations were also made on these same agoutis, and serologic, microbiologic, and endoparasitic tests were made on serum and fecal samples from agoutis maintained at other institutions. streptococci, lactobacilli and nonenteropathogenic escherichia coli were isolated from cecal contents of all ... | 1976 | 185456 |
| quality control for fermented meats. | | 1976 | 187566 |
| a deoxyadenylate kinase activity associated with polynucleotide phosphorylase from micrococcus luteus. | we report here the presence of two enzymatic activities associated with highly purified preparations of polynucleotide phosphorylase from micrococcus luteus. the first, a nuclease activity, which is not separated from the phosphorylase on hydroxylapatite, may be due to substitution of h2o for phosphate in the phosphorolysis reaction. the second activity, a deoxyadenylate kinase, the bulk of which is not resolved from the phosphorylase using gel filtration, sucrose density gradient centrifugation ... | 1976 | 188014 |
| sulfur amino acid auxotrophy in micrococcus species isolated from human skin. | since methionine and (or) cysteine are required by a large percentage of natural auxotrophic micrococcus strains isolated from human skin, investigations were directed to determine the specific enzymes affected in sulfur amino acid biosynthesis. known intermediates in the interrelated cysteine and methionine biosynthetic pathways were tested as growth stimulants. based on these growth studies, sulfur amino acid auxotrophs were grouped into three cysteine classes and five methionine classes. sele ... | 1976 | 188532 |
| interaction of pt(ii) complexes with dnas from various sources. a circular dichroism study. | the interaction of cis- and trans-pt(nh3)2cl2 with dnas of different base composition was studied by means of circular dichroism (cd) spectroscopy. the spectra obtained indicated a partial transformation of the secondary structure of dna (b- less than c transition) with an increased helix winding. subtle differences were noted between the two isomers, possibly related to changes in base orientation due to the different molecular geometries of the p+(ii) complexes. | 1977 | 189945 |
| prophage induction by alkylating ethyl methylaminosulfonate. | treatment of the lysogenic strain m. lysodeikticus 53-40 (n5) with ethyl methylaminosulfonate results in a slight increase in infective center concentration of n5 phages which is only detectable once disturbing cells had been eliminated by lysozyme. after conversion of ethyl methylaminosulfonate with radioactive labelled guanosine at 37 degrees c, n7-ethyl-guanine could be ascertained. therefore the biological activity of the drug may be due to an ethylation of dna. | 1977 | 192005 |
| aspartokinase of micrococcus luteus. 'feedback-stimulation" by methionine. | | 1977 | 192586 |
| specific thiamine monophosphate phosphohydrolase in micrococcus denitrificans. | a phosphohydrolase specific for thiamine monophosphate was isolated from micrococcus denitrificans, partially purified approximately 100-fold, and separated from acid phosphatase. | 1977 | 192716 |
| pivmecillinam in urinary tract infections: a correlation of urinary bactericidal activity with clinical efficacy. | | 1977 | 193813 |
| specificity of dna basic polypeptide interactions. ii+ influence of aromatic amino acid residues investigated with agarose bound lysine copolypeptides. | binding affinities towards dna and base pair specificities of lysine copolymers, containing different amounts of phe, tyr or trp residues, were estimated using a previously described chromatographic method. incorporation of few aromatic residues into polylysine causes a decrease in the binding affinity, however, further raising the aromatic residue - lysine ratio results in a continous increase of affinity, which is most pronounced with the tyr copolymers and not observed with polymers containin ... | 1977 | 194223 |
| [interaction of bacterial h+-atpase with the membrane in a reconstitution system]. | | 1977 | 196683 |
| purification and properties of an acid phosphatase of micrococcus denitrificans distinct from thiamine phosphate phosphatase. | to determine whether the acid phosphatase in micrococcus denitrificans participates in hydrolysis of thiamine phosphate in the synthesis of thiamine pyrophosphate, acid phosphatase was purified 280-fold by conventional procedures, which removed thiamine phosphate phosphatase completely. studies showed that this acid phosphatase is a different protein from thiamine phosphate phosphatase and that it has no binding site for thiamine phosphate on its active site. | 1977 | 197075 |
| processive and synchronous mechanism of polynucleotide phosphorylase phosphorolysis. a comparison between experimental results and those calculated from a theoretical study. | | 1977 | 199439 |
| intracellular distribution of enzymes of phospholipid metabolism in several gram-negative bacteria. | cell-free extracts of salmonella typhimurium, serratia marcescens, enterobacter aerogenes, and micrococcus cerificans contained the following enzymatic activities related to phospholipid metabolism: cytidine 5'-diphospho-1,2-diacyl-sn-glycerol (cdp-diglyceride):l-serine o-phosphatidyltransferase (phosphatidylserine synthase), phosphatidylserine decarboxylase, cdp-diglyceride:sn-glycero-3-phosphate phosphatidyltransferase (phosphatidylglycerophosphate synthase), phosphatidylglycerophosphate phosp ... | 1977 | 199573 |
| dna-relaxing enzyme from micrococcus luteus. | a dna-relaxing enzyme which catalyzes the conversion of superhelical dna to a non-superhelical covalently closed form has been purified from micrococcus luteus to near homogeneity by two chromatographic steps. the enzyme is a single polypeptide chain. as determined by sodium dodecyl sulfate - polyacrylamide gel electrophoresis and gel filtration on sephadex g 150, the molecular weight is 115,000. the dna-relaxing activity determined as a function of enzyme concentration follows a sigmoidal curve ... | 1977 | 202927 |
| structural homology of cytochromes c. | cytochromes c from many eukaryotic and diverse prokaryotic organisms have been investigated and compared using high-resolution nuclear magnetic resonance spectroscopy. resonances have been assigned to a large number of specific groups, mostly in the immediate environment of the heme. this information, together with sequence data, has allowed a comparison of the heme environment and protein conformation for these cytochromes. all mitochondrial cytochromes c are found to be very similar to the cyt ... | 1978 | 203462 |
| the purification and characterization of a dna nicking-closing enzyme from bacillus megaterium. | although several eucaryote dna nicking--closing (n--c) enzymes have been characterized, only the escherichia coli enzyme has been extensively studied amongst procaryotes. the latter enzyme is distinctly different from the eucaryotic enzymes and we have therefore purified the n--c enzyme from bacillus megaterium to determine if procaryotes form a distinctive class of n--c enzymes. the purified b. megaterium n--c enzyme has a molecular weight of 120,000, only partly relaxes negative supercoils, do ... | 1978 | 204401 |
| [new route of microbial degradation of ordram]. | | 1978 | 206419 |
| periodicity and fragment size of dna from mouse tlt hepatoma chromatin and chromatin fractions using endogenous and exogenous nucleases. | the action of micrococcal nuclease, dnase i and dnase ii on mouse tlt hepatoma chromatin revealing the periodicity of its structure as visualized by denaturing and non-denaturing gel electrophoresis, was consistent with the action of these enzymes on other chromatins. micrococcal nuclease showed a complex subnucleosome fragment pattern based on multiples of 10 base pairs with a prominant couplet at 140/160 base pairs and the absence of the 80 base pair fragment. this couplet of the core and mini ... | 1978 | 206820 |
| neutralization of human serum lysozyme by sodium polyanethol sulfonate but not by sodium amylosulfate. | sodium polyanethol sulfonate (sps) at 500 microgram/ml, but not sodium amylosulfate (sas) at 500 microgram/ml, precipitated egg white lysozyme (1 mg and 50 microgram of lysozyme per ml) as determined with the assay strain micrococcus lysodeikticus atcc 4698. fresh and heat-inactivated (56 degrees c, 30 min) human serum (80%, vol/vol) killed m. lysodeikticus (10(4) bacteria per ml at zero time) within 1 to 2 h after exposure. addition of 250 to 500 microgram of sps per ml to fresh human serum pro ... | 1978 | 212454 |
| rubredoxin. | | 1978 | 213684 |
| physical properties of inner histone-dna complexes. | chicken-erythrocyte inner histone tetramer has been complexed with several natural and synthetic dna duplexes by salt-gradient dialysis at various protein/dna ratios. the resulting complexes, in low-ionic-strength buffer, have been examined by electron microscopy, circular dichroism, and thermal denaturation. electron microscopy reveals nucleosomes (nu bodies) randomly arranged along dna fibers, including poly(da-dt)-poly(da-dt), poly(di-dc)-poly(di-dc), but not poly(da)-poly(dt). circular dichr ... | 1978 | 214760 |
| classification of staphylococcus epidermidis and micrococcus strains isolated from bovine milk. | | 1978 | 215064 |
| effect of silver nitrate application on the conjunctival flora of the newborn: and the occurrence of clostridial conjunctivitis. | newborn conjunctival cultures were obtained from 35 babies prior to silver nitrate application and 48 hours later. on initial culture, 46 facultative bacteria and 27 anaerobes were recovered; 48 facultative and 18 anaerobes were recovered after 48 hours. haemophilus vaginalis, bacteroides species and anaerobic cocci decreased in numbers, whereas s. epidermidis, micrococcus and propionibacterium acnes increased during this time interval. clostridial species were isolated from two cases who develo ... | 1978 | 216788 |
| [development of an esr signal with a resolved superthin structure during oxidative processes in bacterial membranes]. | | 1979 | 219906 |
| neutralization of human serum beta-lysin by sodium polyanetholsulfonate and sodium amylosulfate. | normal fresh and heat-inactivated (56 degrees c, 30 min) human sera (80 vol%, i.e., 80% [vol/vol] of a 2-ml assay volume) killed bacillus subtilis atcc 6633 cell inocula of 1.5 x 10(4) colony-forming units per ml within 1 to 2 h after exposure. the b. subtilis assay strain proved slightly and reversibly susceptible to 5 mug of egg white lysozyme per ml. seitz filtration of fresh human serum completely removed beta-lysin activity; significant amounts of serum lysozyme were removed as well, as det ... | 1979 | 227918 |
| variable neutralization of several nonspecific antibacterial systems in fresh, defibrinated human blood by sodium polyanetholsulfonate and sodium amylosulfate. | fresh, defibrinated human blood (80 vol%, i.e., 80% [vol/vol] of a 2-ml final assay volume) from two healthy adult donors killed "delayed serum-sensitive" (dss) and "promptly serum-sensitive" (pss) strains of serratia marcescens, pss control strain escherichia coli c, bacillus subtilis strain atcc 6633, and micrococcus lysodeikticus atcc 4698 in a kinetic manner comparable to that of fresh human serum (80 vol%). however, heat-inactivated (56 degrees c, 30 min), defibrinated human blood revealed ... | 1979 | 227919 |
| binding of platinum(ii) intercalation reagents to deoxyribnonucleic acid. dependence on base-pair composition, nature of the intercalator, and ionic strength. | the dna binding of three platinum(ii) intercalation reagents has been studied and found to depend upon base composition, the nature of the intercalator, and the ionic strength of the solvent medium. in 0.2 m nacl, binding data for calf thymus dna show the association constants to be approximately 10(4) m-1. the binding constants decrease in the order [(o-phen)pt(en)]2+ greater than or equal to [(terpy)pt(het)]+ greater than [(bipy)pt(en)]2+. the number of available intercalation sites for the do ... | 1979 | 229900 |
| a topoisomerase from escherichia coli related to dna gyrase. | we have identified a topoisomerase activity from escherichia coli related to dna gyrase (topoisomerase ii): we designate it topoisomerase ii'. it was constructed of two subunits, which were purified separately. one is the product of the gyra (formerly nala) gene and is identical to subunit a of dna gyrase. the other is a 50,000-dalton protein, which we have purified to homogeneity and call v. v may be a processed form of the much larger gyrase subunit b or may be derived from a transcript of par ... | 1979 | 230498 |
| measurement of pyrimidine dimers in spheroplasts of bacillus subtilis. | a method is described for making spheroplasts of bacillus subtilis which are permeable to exogenous enzymes. conditions are described for measuring small numbers of pyrimidine dimers in the dna of uv-irradiated cells by use of a partially purified micrococcus luteus extract containing an enzyme specific for pyrimidine dimers. the system will detect as few as 10-12 pyrimidine dimers per genome. | 1979 | 232234 |
| studies on the substrate specificity of the dna methylase activity from escherichia coli k-12. | a partially purified extract of dna methylases from e. coli k-12 containing dna-adenine as well as dna-cytosine methylase activities has been examined with respect to different dna species as substrates. the results show that the natural content of 6-map) in the applied dna represses the dna-adenine methylase activity. on the other hand, 5-mc, already present in the substrate does not influence the activity of the dna-cytosine methylase. dna from micrococcus radiodurans, which is completely free ... | 1979 | 232591 |
| [the effect of sodium polyanethol sulfonate (sps) on the growth of bacteria in blood cultures]. | the effect of sps on growth of bacteria in blood cultures were studied. for that reason, 752 blood cultures (in media with and without sps) were detected and found that sps may help to growth only micrococcus species. | 1979 | 233490 |
| in vitro metabolic conversion of aflatoxins and benzo(alpha)pyrene to nucleic acid-binding metabolites. | an aflatoxin b1 metabolite was found to become covalently bound to rat liver rna and calf thymus dna in vitro, and it formed complexes with increased spectral absorbance in the 360 nm region. the formation of such complexes was reduced nicotinamide adenine dinucleotide phosphate and microsome dependent, was inhibited by theta-diethylaminoethyl diphenylpropylacetate-hc1, and by co and n2, when the latter were used to replace the gas phase of the incubations. the formation of the complexes was enh ... | 1975 | 234034 |
| enzyme electrophoretograms in the analysis of taxon relatedness of micrococcus cryophilus, branhamella catarrhalis and atypical neisserias. | extracts were prepared from micrococcus cryophilus, several strains of branhamella catarrhalis and neisseria spp. esterases, nadp-dependent isocitrate dehydrogenase and malate dehydrogenase activities were assayed after electrophoresis of extracts of polyacrylamide gels. except for neisseria perflava and n. sicca which resolved activity bands for the acetate-esterase only, the remaining bacteria exhibited species-specific esterase patterns also for the propionate and butyrate substrates. the mul ... | 1975 | 234507 |
| paracoccus denitrificans and the evolutionary origin of the mitochondrion. | | 1975 | 235742 |
| analysis of results of catheter tip cultures in open-heart surgery patients. | a statistical analysis of 628 consecutive catheter tip cultures is presented. all were from patients undergoing open-heart surgery. the previously noted effects of early removal are shown to be significant. the possible effects of stasis, flushing, handling, and place of insertion are discussed. the unresolved significance of isolations of aerobic sporing bacilli is noted, and a decreasing incidence of postoperative infective endocarditis in the same group of patients is an encouraging sign. | 1975 | 235801 |
| an extracellular 5'-nucleotidase with both monoesterase and diesterase activity from micrococcus sodonensis. | | 1975 | 235884 |
| purification and characterization of an endonuclease from calf thymus acting on irradiated dna. | an endonuclease acting on dna exposed to ultraviolet light or gamma-rays has been extensively purified from calf thymus. the enzyme has a ph optimum at ph 7.0-7.5, acts with equal efficiency in the presence of edta or divalent cations (mg-2+ or ca-2+), is inhibited by nacl and trna and is inactivated by incubation at 50 degrees c. its molecular weight, determined by sephadex chromatography or sodium dodecylsulfate gel electrophoresis, is approx. 30 000. the enzyme catalyzes the formation of brea ... | 1975 | 236041 |
| a deoxyribonuclease of diplococcus pneumoniae specific for methylated dna. | a deoxyribonuclease specific for methylated dna was isolated from diplococcus pneumoniae. the enzyme, an endonuclease, degrades dna for escherichia coli to fragments of average molecular weight about half a million; it forms discrete fragments from phage lambda dna. methyl-deficient e. coli dna is not attacked, neither is dna from micrococcus radiodurans, which contains no methylated adenine or cytosine. nor is dna from d. pneumoniae or phage t7 attacked. however, dna from m. radiodurans, d. pne ... | 1975 | 236309 |
| [some properties of beta-aspartate kinase from micrococcus glutamicus-95 bacteria]. | kinetic and allosteric properties of beta-asparatatekinase from micrococcus glutamicus-95 were studied. coarse protein fraction, sedimented at 0.8 from saturated (nh4)2so4, was used as an enzyme preparation. curves of the dependency of the enzymatic reaction rate on substrate (l-aspartic acid and atp) concentration were not found to be s-like. however, double reciprocal plotting of the data obtained revealed their deviation from the hyperbolic curve. the effect of amino acids (lysine, threonine, ... | 1975 | 237584 |
| isolation and structure assignment of an antimicrobial principle from the volatile oil of nigella sativa l. seeds. | refrigeration of the volatile oil of nigella sativa l. seeds eventuates in a crystalline substance. the chemical structure of the compound was drawn from its chemical behaviour, as well as from its uv, ir, pmr and mass spectral data. the compound was found to be thymohydroquinone: confirmation of the structure was established via the preparation of its corresponding diacetate ester. the compound was found to have high antimicrobial effect against gram positive microorganisms | 1975 | 238225 |
| the identification and biosynthesis of siderochromes formed by micrococcus denitrificans. | 1. micrococcus denitrificans excretes three catechol-containing compounds, which can bind iron, when grown aerobically and anaerobically in media deficient in iron, and anaerobically in medium with a high concentration of ca2+. 2. one of these compounds was identified as 2,3-dihydroxybenzoic acid (compound i), and the other two were tentatively identified as n1n8-bis-(2,3-dihydroxybenzoyl)spermidine (compound ii) and 2-hydroxybenzoyl-n-l-threonyl-n4[n1n8-bis-(2,3-dihydroxybenzoyl)]spermidine (co ... | 1975 | 238503 |
| the localization and distribution of gram-positive cocci in normal skin and in lesions of acne vulgaris. | the localization of gram-positive cocci in the normal skin and in the lesions of acne vulgaris was investigated using fluorescein-labeled antiserum raised to gram-positive, coagulase-negative cocci. the cocci were found in 10 of 19 specimens from normal facial skin and in 3 of 11 specimens from the normal skin of the rest of the body. the bacteria were found mostly in the openings of follicles, but in 6 of 10 facial skin specimens, they were also present deeply in the lumina of the dilated sebac ... | 1975 | 239073 |
| the bacteriological ecosystem of the skin of children in an african tropical envirovment (tanzania). | using a standardized method for sampling, the aerobic microbial flora of the skin of ninety-five children in dar es salaam, tanzania, was studied qualitatively and quantitatively. the subjects comprised three groups; group a, thirty-seven normal infants (below the age of 1 year); group b, twenty-nine infants with various bacterial skin lesions; and group c, twenty-nine nursery school children between 4-5 and 7 years. the following observations were made: (i) the quantitative results indicated; ( ... | 1975 | 239739 |
| purification and characterization of a streptomyces albus endo-n-acetylmuramidase lytic for group a and other beta haemolytic streptococci. | the purification and characterization of the streptolytic exo-enzyme from the maxted-mccarty strain of streptomyces albus is described. this enzyme was shown to be an endo-n-acetylmuramidase with a molecular weight of 10 to 12,000 and optimal activity at ph 8 and 45 degrees c. the enzyme is lytic for streptococci of various groups, micrococcus lysodeikticus, staphylococcus aureus, as well as escherichia coli. it closely resembles the f1 endo-n-acetylmuramidase described by ghuysen et al. (1966) ... | 1975 | 240102 |
| d-alanine carboxypeptidase activity of micrococcus lysodeikticus released into the protoplasting medium. | conversion of whole cells of micrococcus lysodeikticus to protoplasts allowed the release of a soluble form of a d-alanine carboxypeptidase into the protoplasting medium. the enzyme cleaves the terminal d-alanine from the radioactively labelled udp-n-acetylmuramyl-pentapeptide containing l-lysine as the diamino acid. however, the enzyme is only minimally active in this fraction so that it had to be enriched and partially purified before its properties could be studied. chromatography on carboxym ... | 1975 | 240694 |
| enzymic and immunochemical properties of lysozyme. x. conformation, enzymic activity and immunochemistry of lysozyme reduced at two carboxyl groups. | reduction of lysozyme by diborane, followed by air oxidation of the reduced disulfides and chromatography on cm-cellulose, yielded a homogeneous derivative. in the derivative, the carboxyl groups of aspartic acid 119 and the end-chain leucine residue were reduced to their corresponding alcohols. correct re-forming of the disulfide bonds was demonstrated by peptide mapping of the tryptic hydrolysates of the derivative and lysozyme without breaking the disulfide bonds, followed by identification o ... | 1975 | 241414 |
| improved lysozyme assay in biological fluids. | we describe a simple, rapid, sensitive, and highly reproducible assay for lysozyme, with use of concentrated cell suspensions of micrococcus lysodeikticus in tris-buffered glycerol/water (40/60 by vol), ph 7.5. stored at -20 degrees c, the cells' susceptibility to lysozyme remains unaltered over long periods. almost identical concentration curves were obtained with different aliquots of the same preparation during eight months. lysozyme activity was reflected in the decrease in absorbance of the ... | 1979 | 262192 |
| ecological effects of a deodorant and a plain soap upon human skin bacteria. | the effects of a commercial trichlorocarbanilide-containing deodorant soap and a commercial plain soap upon the cutaneous flora of individuals were compared. using a cross-over design, 21 volunteers (10 women and 11 men) washed their forearms at least once a day with one soap for 3 weeks and then switched soaps for another 4 weeks use. by analysis of variance no significant difference in total colony counts was noted among individuals in their use of the two soaps. with the exception of individu ... | 1977 | 264493 |
| electrophoresis of lysozyme into microscoccus-containing agarose gel: quantitative and analytical applications. | electrophoresis of lysozyme into agarose gel containing micrococcus lysodeikticus causes lysis of the microorganism, allowing the development of two methods, one for quantitation ("lyso-rocket electrophoresis") and the other for electrophoretic characterization ("crossed lyso-electrophoresis") of lysozyme. the lyso-rocket technique provides an alternative to the method currently used for quantitative assay. by the use of crossed lyso-electrophoresis we have provided evidence, for the first time, ... | 1977 | 265197 |
| different rate-limiting steps in excision repair of ultraviolet- and n-acetoxy-2-acetylaminofluorene-damaged dna in normal human fibroblasts. | in normal human cells the amount of excision of ultraviolet damage to dna saturates at high doses. in these cells some chemicals mimic ultraviolet damage as far as their biological and repair characteristics are concerned. one of these chemicals is n-acetoxy-2-acetylaminofluorene. we determined whether the limited repair capacity for ultraviolet damage was affected by treatment with n-acetoxy-2-acetylaminofluorene. to measure repair we determined unscheduled dna synthesis and the number of sites ... | 1977 | 266195 |
| micrococcus luteus dna gyrase: active components and a model for its supercoiling of dna. | two active components alpha and beta of micrococcus luteus dna gyrase, of peptide weights of 115,000 and 97,000, respectively, have been purified. each individual component exhibits little dna gyrase activity; the atp-dependent negative supercoiling of a covalently closed circular dna duplex is catalyzed by a combination of the two. covalent closure by escherichia coli ligase of a circular dna containing single-chain scissions, when carried out in the presence of a combination of the dna gyrase ... | 1978 | 276855 |
| determination of membrane properties at the molecular level by carbon-13 and deuterium magnetic resonance. | | 1978 | 279301 |
| topographic study of the cell surface of micrococcus radiodurans. | the paracrystalline outer membraneous layer (hpi layer) of micrococcus radiodurans has been investigated by negative- and positive-staining electron microscopy and subsequent digital image processing. the subunit structure of the major hpi layer protein complex and the lipid-protein distribution in the plane of the membrane have been determined. the hpi layer was found to be highly asymmetric in a transmembrane direction, with the major protein complex only partly penetrating into a lipid-contai ... | 1978 | 281701 |
| [contamination of surgical wounds in the maxillofacial region with microoranisms]. | the analysis of 226 wound smears were statistically evaluated. smears were taken immediately after beginning surgery (105) and during the course of the operation, usually toward the end (121). no bacteria were identified at the beginning of the operation in 11.6% of the smears; 8.6% of the smears taken toward the end of the operative procedure showed no bacteria. microorganisms usually found in the buccal cavity were identified in approximately two thirds of the wounds. every third or fourth wo ... | 1978 | 282987 |
| bacterial contamination of radial artery catheters. | a prospective survey of 113 radial artery catheters showed a total contamination rate using an enrichment media culturing technique of 39%. the yield from routine culturing alone was only 9%. in six cases pathogenic bacteria were isolated. in this study no correlation between contamination and aspects of catheter insertion and management was shown. | 1979 | 286896 |
| lysozyme activity in saliva from children with various degree of gingivitis. | the lysozyme activity was determined in paraffin stimulated whole saliva from 51 randomly selected children. the average age was 6.5 years. the children were divided into four groups according to their degree of gingival inflammation. an agardiffusion method was used with micrococcus lysodeikticus as indicator of the lysozyme activity. the mean value of the lysozyme activity was determined to 48.4 microgram/ml. in the non-inflamed group the enzyme activity was 8.7 microgram/ml, whereas it was lo ... | 1979 | 288181 |
| endonucleolytic activity directed towards 8-(2-hydroxy-2-propyl) purines in double-stranded dna. | photoalkylation of circular covalently closed dna from phage pm2 with isopropyl alcohol by using a free radical photoinitiator and uv light of lambda greater than 305 nm led to the specific 8-substitution of purine moieties in the dna, yielding 8-(2-hydroxy-2-propyl)adenine and 8-(2-hydroxy-2-propyl)guanine as the only detectable damage in the dna. using this specifically photoalkylated dna as a substrate, we discovered in extracts of micrococcus luteus an endonucleolytic activity that is direct ... | 1979 | 293658 |
| micrococcus luteus pneumonia: a case report and review of the literature. | the clinical course of a 69-year-old male with acute myelogenous leukemia is described who, while extremely leukopenic (less than 100 neutrophils/microliter) from chemotherapy, developed a cavitating pneumonia due to a gram-positive coccus, micrococcus luteus. aggressive antibiotic management and attainment of complete remission of his leukemia resulted in a successful outcome. a review of the literature regarding the pathogenicity of this organism and, in particular, its occurrence as a cause o ... | 1979 | 296784 |
| endonuclease activities in extracts of micrococcus luteus that act on gemma-irradiated dna. | several protein fractions containing endonuclease activity against gemma-irradiated dna (gamma-endonuclease) were isolated from m. luteus. the crude extract was eluted on a phosphocellulose column and chromatographed on teae cellulose and subsequently on hydroxyapatite. five peaks of gamma-endonuclease were obtained from each preparation. repeated experiments showed comparable chromatographic behavior of the fractions. there was no detectable activity of u.v.-endonuclease in the fractions with g ... | 1977 | 300726 |
| effects of oxygen during gamma-irradiation of micrococcus radiodurans. | | 1977 | 303627 |
| idiotypic regulation of the immune system by the induction of antibodies against anti-idiotypic antibodies. | anticarbohydrate antibodies (ab1) were isolated from a rabbit hyperimmunized with micrococcus lysodeikticus and injected into allotype-matched rabbits in order to obtain specific anti-iodiotypic antibodies (ab2). ab2 was isolated by means of a sepharose column coupled to the anticarbohydrate antibodies and was injected into two allotype-matched rabbits. these latter rabbits produced specific anti-anti-idiotypic antibodies (ab3) probably sharing idiotypic specificities with ab1. however, these ab ... | 1977 | 303775 |
| flocculation and adsorption of enzymes during growth of a moderate halophile, micrococcus varians var. halophilus. | flocculation of a moderate halophile, micrococcus varians atcc 2197, occurred during growth in complex medium containing 3 m nacl and a concentration of mgso4 and kh2po4 greater than 40 and 14 mm, respectively. extracellular nuclease activity was absent in the flocculated cultures. repeated washing of flocs by mg2+-free tris buffer containing 3 m nacl, lowering of ph value of floc suspension below 6.3, or addition of ethylenediaminetetraacetic acid resulted in complete dissociation of the flocs ... | 1978 | 307427 |
| irradiation of micro-organisms with mono-energetic x-rays; biological consequences of the auger effect. | the radiation resonance effect reported previously for isolated biomolecules has now for the first time been observed in a cellular system. dried bacteria, micrococcus denitrificans, in which tdr in dna was partially substituted by budr, were subjected to mono-energetic x-rays of energies below or above the k-edge for br. subsequently, the colony-forming ability was assayed. for photon energy slightly above the k-edge, the lethality/rad was greater than that below the k-edge. this is interpreted ... | 1978 | 309443 |
| idiotypic regulation of the immune system. common idiotypic specificities between idiotypes and antibodies raised against anti-idiotypic antibodies in rabbits. | anti-idiotypic antibodies (ab2) were raised in allotype-matched rabbits against anti-carbohydrate or anti-tobacco mosaic virus antibodies (ab1). several ab2 were purified and injected into a third series of rabbits iii which synthesized antiantiidiotypic antibodies (ab3). antigen was then given for the first time in those rabbits who had synthesized ab3. the specific antibody synthesized in rabbits iii was called ab1'. anti-idiotypic antibodies were raised against purified ab3 antibodies (ab4). ... | 1979 | 312906 |
| autoregulation of an antibody response via network-induced auto-anti-idiotype. | the antibody response of a single outbred rabbit was studied throughout three rounds of injections with micrococcus lysodeikticus vaccine over a 31-mo period. the first-round response was characterized by a vigorous anti- micrococcus response and a strong anti-igg rheumatoid factor response. the second-round response consisted of a triad of interacting molecules: anti- micrococcal antibodies, autoanti-idiotypic antibodies specific for distinct clonotypes of the first-round anti-micrococcal antib ... | 1979 | 312909 |
| excision repair of gamma-ray-induced alkali-stable dna lesions with the help of gamma-endonuclease from micrococcus luteus. | gamma-endonuclease y, an enzyme that hydrolyses phosphodiester bonds at alkalistable lesions in gamma-irradiated (n2, tris buffer) dna, has been partially purified from micrococcus luteus. the enzyme has a molecular weight of about 19 000, induces single-strand breaks with 3'oh-5'po4 termini and contains endonuclease activity towards dna treated with 7-bromomethylbenz(a)anthracene. gamma-endonuclease y induces breaks in oso4-treated poly(da-dt) and apparently is specific towards gamma-ray-induce ... | 1979 | 313380 |
| a rapid method for detecting base damage in dna of mammalian cells: assay of u.v.-induced pyrimidine dimers in human cells. | | 1978 | 313915 |
| influence of cationic triphenylmethane dyes upon dna polymerization and product hydrolysis by escherichia coli polymerase i. | the cationic triphenylmethane dyes crystal violet, methyl green, and malachite green inhibited dna synthesis catalyzed by escherichia coli b polymerase i (polymerase i). lower concentrations of the dyes inhibited dna replication as a direct function of the proportion of at to gc in the dna of clostridium perfringens, escherichia coli, and micrococcus lysodeikticus. when the intercalant proflavin was employed, the gc-rich micrococcal dna was most severely inhibited. in addition, both the tripheny ... | 1977 | 318853 |
| aerobic and anaerobic bacterial respiration monitored by electrodes. | a technique is described by which both oxygen and nitrate (or nitrate or chlorate) levels were continuously monitored during bacterial respiration. paracoccus (micrococcus) denitrificans and escherichia coli oxidizing succinate rapidly ceased to reduce nitrate when oxygen was available, and equally rapidly commenced nitrate reduction when all the oxygen had been consumed. by contrast, membrane vesicles isolated from p. denitrificans reduced oxygen and nitrate simultaneously. the respiratory nitr ... | 1977 | 319200 |
| preparation of escherichia coli trnas terminating of modified nucleosides by the use of ctp(atp):trna nucleotidyltransferase and polynucleotide phosphorylase. | two procedures were investigated for the modification of trnas at the 3'-terminal nucleoside. the first involved the incubation of an enzymatically abreviated trna (trna-c-coh) with appropriate nucleoside triphosphates in the presence of ctp(atp):trna nucleotidyltransferase from escherichia coli and yeast. the e. coli enzyme did not utilize 2'- or 3'-deoxyadenosine 5'-triphosphate as substrates, but affected incorporation of the 2'- and 3'-o-methyladenosine triphosphates onto trna-c-cou to the e ... | 1977 | 319825 |
| the functional capacity of guinea pig megakaryocytes. ii. the uptake of particles and macromolecules and the effect of rabbit antiguinea pig platelet antiserum. | the capacity of megakaryocytes to take up particles and macromolecules was tested by exposing them to homologous erythrocytes, latex particles, micrococcus lysodeikticus, and horseradish peroxidase. uptake of particles 1.1 to 7 mum. in size was extensive under the experimental conditions employed. after uptake of each of the three particles the addition of tannic acid and calcium to the fixative produced electron-dense deposits within the vacuole surrounding the particle; therefore, the vacuole ... | 1977 | 320386 |
| a simple procedure for determination of bacteriolytic activity in biological fluids. | a new procedure for spectrophotometric determination of bacteriolytic activity in biological fluids is described. the method uses micrococcus lysodeikticus cells as substrate. by inactivation of the sample through pre-incubation in ice-water, a large number of samples can be prepared and analysed simultaneously. the necessity of performing the whole analysis in the photometer for one sample at the time is thus eliminated. by measuring the increase in transmission at 570 nm after incubation of th ... | 1977 | 320821 |
| deoxyribonucleic acid repair in vitro by extracts of escherichia coli. | deoxyribonucleic acid (dna) from bacteriophage t7 has been used to monitor the capacity of gently lysed extracts of escherichia coli to perform repair resynthesis after ultraviolet (uv) irradiation. purified dna damaged by up to 100 j of uv radiation per m2 was treated with an endonuclease from micrococcus luteus that introduces single-strand breaks in irradiated dna. this dna was then used as a substrate to study repair resynthesis by extracts of e. coli. it was found that incubation with the e ... | 1977 | 321426 |
| continuous-culture studies of interactions among human skin-commensal bacteria. | chemostat studies were made of an antibiotic-producing staphylococcus epidermidis strain (s6+), a similar, but antibiotic non-producing s. epidermidis strain (s6-), and a sensitive indicator strain of micrococcus sp. (m7). pure and mixed continous cultures were investigated at low population levels (in 0-1% peptone water) and at higher levels (in 0-5% peptone water). strain s6+ antagonised the growth of strain m7 when its colony count was maintained above 10(7-0) per ml, while strain s6- remaine ... | 1977 | 323493 |
| [cultural determination of anaerobic cocci especially micrococcus indolicus, in milk samples from heifers with the so-called summer mastitis]. | | 1977 | 324739 |
| laboratory assessment of physical and chemical methods of preserving urine specimens. | three methods of preserving simulated specimens of urine were studied with six test strains of bacteria. viable counts were measured by a surface viable count and by the filter-paper-strip method during a holding period of 72 hours. refrigeration at approximately 4 degrees c was effective and reliable. boric acid (1-8%) at room temperature was toxic for the strain of escherichia coli at a density of 10(7) cfu/ml but this may not be significant at the higher concentration of bacterial cells often ... | 1977 | 326817 |
| topological asymmetry of phospholipids in membranes. | | 1977 | 327544 |
| antibacterial effects of some sugar hydrazones. | | 1977 | 328358 |
| host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli. | the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ... | 1977 | 329101 |
| micrococcus luteus correndonucleases. ii. mechanism of action of two endonucleases specific for dna containing pyrimidine dimers. | py pyrimidine dimers py correndonucleases i and ii from micrococcus luteus act exclusively on thymine-thymine, cytosine-cytosine, and thymine-cytosine cyclobutyl dimers in dna, catalyzing incision 5' to the damage and generating 3'-hydroxyl and 5'-phosphoryl termini. both enzymes initiate excision of pyrimidine dimers in vitro by correxonucleases and dna polymerase i. the respective incised dnas, however, differ in their ability to act as substrate for phage t4 polynucleotide ligase or bacterial ... | 1977 | 330526 |
| a high efficiency flow cytometer. | a flow chamber has been developed which collects about 60% of the total cell fluorescence for analysis compared to about 2.5% for conventional flow systems. the chamber, an ellipsoid of revolution, is gold-plated for increased reflectivity. fluorochrome-stained cells enter the flow cell directly above the primary focus of the ellipsoid at the rate of 1000 cell/sec. a focused argon-ion laser beam enters the flow cell parallel to the semiminor axis and intersects the cell stream at the primary foc ... | 1977 | 330729 |
| in vitro packaging of uv radiation-damaged dna from bacteriophage t7. | when dna from bacteriophage t7 is irradiated with uv light, the efficiency with which this dna can be packaged in vitro to form viable phage particles is reduced. a comparison between irradiated dna packaged in vitro and irradiated intact phage particles shows almost identical survival as a function of uv dose when escherichia coli wild type or pola or uvra mutants are used as the host. although uvra mutants perform less host cell reactivation, the pola strains are identical with wild type in th ... | 1977 | 330877 |
| repair of pyrimidine dimers in radiation-sensitive mutants rad3, rad4, rad6 and rad9 of saccharomyces cerevisiae. | the ability to remove ultraviolet (uv)-induced pyrimidine dimers was examined in four radiation-sensitive mutants of saccharomyces cerevisiae. the susceptibility of dna from irradiated cells to nicking by either the t4 uv-endonuclease or an endonuclease activity found in crude extracts of micrococcus luteus was used to measure the presence of dimers in dna. the rad3 and rad4 mutants are shown to be defective in dimer excision whereas the rad6 and rad9 mutants are proficient in dimer excision. | 1977 | 335235 |
| formation of a rna polymerase sub-assembly composed of subunit alpha from escherichia coli and of subunit beta from micrococcus luteus. | functionally equivalent subunits of rna polymerase from micrococcus luteus and escherichia coli differ from each other in many molecular and antigenic properties. in spite of these differences, subunit alpha from e. coli and subunit beta from m. luteus form a complex alpha2beta, when incubated together. this complex binds rifampicin tightly, which the isolated subunits do not. the hybrid complex is very similar in its properties to the complex alpha2beta formed only from e. coli or m. luteus sub ... | 1977 | 338461 |
| infection of the sheep headfly, hydrotaea irritans with bacterial isolates from field cases of summer mastitis. | | 1977 | 339478 |
| [enzymology of dna repair processes]. | | 1977 | 341447 |
| comparison of agar sausage, alginate swab and adhesive tape methods for sampling flat surfaces contaminated with bacteria. | | 1977 | 345694 |
| 1,10-phenanthroline-cuprous ion complex, a potent inhibitor of dna and rna polymerases. | | 1978 | 346019 |
| removal of pyrimidine dimers from saccharomyces cerevisiae nuclear dna under nongrowth conditions as detected by a sensitive, enzymatic assay. | a sensitive and quantitative procedure for the detection of pyrimidine dimers in yesast nuclear dna is described. the assay employs dimer-specific, endonuclease activities from micrococcus luteus together with dna sedimentation through calibrated, alkaline sucrose gradients to detect endonuclease-induced, single-strand breaks. breaks were induced in a dose-dependent manner from 0 to 80 j m-2 at 254 nm and in numbers equivalent to the numbers of dimers induced by similar doses (unrau et al., bioc ... | 1978 | 347280 |
| [disinfection of effluents from municipal sewage treatment plants with hydrogen peroxide (author's transl)]. | effluents from different sewage treatment plants were disinfected, using hydrogen peroxide in concentrations varying between 500 and 50,000 ppm. with a concentration of 5,500 ppm h2o2 and a contact time of 2 h more than 99% of bacteria were killed, including enterobacteria, total and fecal coliforms (fig. 1 a, b, c and d) and fecal streptococci (fig. 2 b). staphylococci and micrococci were less sensitive and contact times of 2 h were not sufficient to kill 99% of this group (fig. 2 a). highly re ... | 1978 | 349977 |