microangiopathic haemolytic anaemia and experimental tumour-cell emboli. | | 1974 | 4845651 |
regulation of thymidine metabolism in escherichia coli k-12: optimal conditions for the assay of 1,5-phosphodeoxyribomutase in ultrasonic extracts. | assay conditions have been established which allow maximum expression of phosphodeoxyribomutase activity in ultrasonic extracts of escherichia coli k-12. the enzyme requires 2-mercaptoethanol, manganous ions, and glucose 1,6-diphosphate for optimal activity. when cells are grown in minimal medium with glycerol as the carbon source and supplemented with 10(-3)m thymidine, phosphodeoxyribomutase is induced three- to four-fold. cell pellets may be frozen for 24 hr before sonic disruption without lo ... | 1970 | 4919987 |
studies on liver regeneration. ii. effects of phenobarbital on the onset and pattern of rat liver regeneration following partial hepatectomy. | | 1971 | 4947050 |
[characteristics and localization of lipids in the abomasum wall of the suckling calf]. | | 1971 | 4949924 |
action of nalidixic acid and hydroxyurea on two blue-green algae. | | 1971 | 5003889 |
epidemiological implications of rubella "vaccine failures". | | 1972 | 5010549 |
new evidence for an -andrenergic component in the sympathetic centres: centrally mediated decrease in sympathetic tone by l-dopa and its antagonism by piperoxane and yohimbine. | | 1972 | 5026404 |
isolation and characterization of lymphocyte plasma membranes. | | 1972 | 5038272 |
specific uptake of radioactivity from 3 h-oxytocin by surviving segments of rat mammary gland. | | 1972 | 5063545 |
sex differences in "emotionality" and behavior of rats in the open-field. | | 1972 | 5076646 |
plasmid transfer in pediococcus spp.: intergeneric and intrageneric transfer of pip501. | transfer of the broad-host-range resistance plasmid pip501 from streptococcus faecalis to pediococcus pentosaceus and pediococcus acidilactici occurred between cells immobilized on nitrocellulose filters in the presence of dnase. expression of the pip501-linked erythromycin and chloramphenicol resistance determinants was observed in transconjugants. intrageneric transfer of pip501 from a p. pentosaceus donor to various pediococcal recipients occurred at frequencies of 10(-4) to 10(-7) transconju ... | 1983 | 6311098 |
a numerical taxonomic study of lactic acid bacteria from vacuum-packed beef, pork, lamb and bacon. | a numerical taxonomic study using 79 unit characters has been performed on 100 isolates of lactic acid bacteria from refrigerated vacuum-packed beef, pork, lamb and bacon. three clusters were observed at 78% s which contained all the strains apart from three unidentifiable streptobacteria, one leuconostoc, and one strain of pediococcus pentosaceus. one cluster (iii) consisted of only one strain of leuc. paramesenteroides and six unidentifiable leuconostoc strains. the two largest clusters (i and ... | 1984 | 6706886 |
in vitro inhibition of helicobacter pylori nctc 11637 by organic acids and lactic acid bacteria. | in this study the effects of both ph and organic acids on helicobacter pylori nctc 11637 were tested. lactobacillus acidophilus, lact. casei, lact. bulgaricus, pediococcus pentosaceus and bifidobacterium bifidus were assayed for their lactic acid production, ph and inhibition of h. pylori growth. a standard antimicrobial plate well diffusion assay was employed to examine inhibitory effects. lactic, acetic and hydrochloric acids demonstrated inhibition of h. pylori growth in a concentration-depen ... | 1995 | 7592140 |
[effect of fasting and explorative laparotomy on body weight, plasma volume and protein balance in the dog]. | | 1974 | 4458354 |
effect of three commercial starters on growth of staphylococcus aureus and enterotoxins (a-d) and thermonuclease production in broth. | the growth of four enterotoxigenic staphylococcus aureus strains was partially inhibited by three commercial starters used in the meat sausage industry when grown in apt broth at 30 degrees c statically. starter sp318 (a mixture of selected strains of lactobacillus sake, pediococcus pentosaceus and staphylococcus xylosus) showed the most inhibitory activity. staphylococcal enterotoxins (a, b, c1 and d) synthesis was totally inhibited by the growth of the three starters, whereas staphylococcal th ... | 1994 | 7703025 |
production and stability of pediocin n5p in grape juice medium. | the production and stability of pediocin n5p from pediococcus pentosaceus, isolated from wine, were examined in grape juice medium. maximum growth and higher titre (4000 u ml-1) were observed at a initial ph of 7.5 and 30 degrees c. the activity of the inhibitory substance was stable between ph values from 2.0 to 5.0 at 4 degrees and 30 degrees c. at ph 10.0 it was completely inactivated. when submitted to 30 min at 80 degrees, 100 degrees and 115 degrees c, maximal stability was observed at ph ... | 1995 | 7759379 |
udp-n-acetylmuramyl-l-alanine functions as an activator in the regulation of the escherichia coli glutamate racemase activity. | d-glutamate is an essential component of the bacterial peptidoglycan. in escherichia coli, the biosynthesis of d-glutamate is catalyzed by a glutamate racemase (encoded by the dga gene) and is regulated by udp-n-acetylmuramyl-l-alanine [doublet et al. (1994) biochemistry 33, 5285], a bacterial peptidoglycan subunit precursor. investigation was conducted to elucidate the interaction between the enzyme and its regulator. whole and n-terminal truncated enzymes, encoded by individual constructs cont ... | 1995 | 7873525 |
bacterial glutamate racemase has high sequence similarity with myoglobins and forms an equimolar inactive complex with hemin. | glutamate racemase (ec 5.1.1.3), an enzyme of microbial origin, shows significant sequence homology with mammalian myoglobins, in particular in the regions corresponding to the e and f helices, which constitute the heme binding pocket of myoglobins. glutamate racemase binds tightly an equimolar amount of hemin, leading to loss of racemase activity. although this enzyme shows homology with aspartate racemase, the latter does not bind hemin. the glutamate racemase gene of pediococcus pentosaceus h ... | 1994 | 7937852 |
pediocin a, a bacteriocin produced by pediococcus pentosaceus fbb61. | pediocin a, a bacteriocin produced by pediococcus pentosaceus fbb61, was purified and partially characterized. the purification was achieved by dialysis against peg 20,000, butanol extraction and electroendosmotic preparative electrophoresis. the protocol led to a 7843-fold increase in the specific activity, with 3.9% activity recovered. sds-page of pediocin a resulted in a single 80 kda protein band. the antimicrobial compound was sensitive to proteolytic enzymes and heat (10 min at 100 degrees ... | 1994 | 8012591 |
modification of peptidoglycan precursors is a common feature of the low-level vancomycin-resistant vanb-type enterococcus d366 and of the naturally glycopeptide-resistant species lactobacillus casei, pediococcus pentosaceus, leuconostoc mesenteroides, and enterococcus gallinarum. | the biochemical basis for the acquired or natural resistance of various gram-positive organisms to glycopeptides was studied by high-pressure liquid chromatographic analysis of their peptidoglycan udp-murnac-peptide precursors. in all cases, resistance was correlated with partial or complete replacement of the c-terminal d-ala-d-ala-containing udp-murnac-pentapeptide by a new precursor with a modified c terminus. nuclear magnetic resonance analysis by sequential assignment showed that the new pr ... | 1994 | 8157610 |
pediococcus pentosaceus catheter-associated infection in an infant with gastroschisis. | | 1994 | 8170738 |
vancomycin binding to cell walls of non-streptococcal vancomycin-resistant bacteria. | the availability of peptidoglycan for binding to vancomycin was investigated in intrinsically vancomycin-resistant clinical isolates of leuconostoc mesenteroides, lactobacillus viridescens and pediococcus pentosaceus. intrinsic vancomycin resistance expressed by l. mesenteroides and p. pentosaceus was the result of poor binding of vancomycin to native or sds-treated cell walls. pre-exposure of l. viridescens to vancomycin decreased the subsequent binding of vancomycin to the cell walls, suggesti ... | 1993 | 8288496 |
functional analysis of the pediocin operon of pediococcus acidilactici pac1.0: pedb is the immunity protein and pedd is the precursor processing enzyme. | the bacteriocin pediocin pa-1 operon of pediococcus acidilactici pac1.0 encompasses four genes: peda, pedb, pedc and pedd. transcription of the operon results in the formation of two overlapping transcripts, probably originating from a single promoter upstream of peda. the major transcript comprises peda, pedb, and pedc, while a minor transcript encompasses all of these genes and pedd. by deletion analysis and overexpression of pedb in pediococcus pentosaceus we demonstrate that this gene encode ... | 1995 | 8559070 |
the antimicrobial activity of lactic acid bacteria from fermented maize (kenkey) and their interactions during fermentation. | a total of 241 lactic acid bacteria belonging to lactobacillus plantarum, pediococcus pentosaceus, lactobacillus fermentum/reuteri and lactobacillus brevis from various processing stages of maize dough fermentation were investigated. results indicated that each processing stage has its own microenvironment with strong antimicrobial activity. about half of the lact. plantarum and practically all of the lact. fermentum/reuteri investigated were shown to inhibit other gram-positive and gram-negativ ... | 1995 | 8567490 |
conservation of the major cold shock protein in lactic acid bacteria. | primers designed from consensus regions of the major cold shock gene of different bacterial species were used in pcr amplification of lactic acid bacteria (lab). an appropriately-sized pcr product was obtained from lactococcus lactis subsp. lactis ll43-1 and mg1363; lactococcus lactis subsp. cremoris lc10-1, lc11-1, and lc12-1; streptococcus thermophilus st1-1; enterococcus faecalis ef1-1; lactobacillus acidophilus la1-1; lactobacillus helveticus lh1-1; pediococcus pentosaceus pp1-1; and bifidob ... | 1998 | 9767713 |
polysaccharide production by pediococcus pentosaceus from wine. | two polymer-forming strains of pediococcus pentosaceus from argentinian wines were grown under different conditions. polysaccharides were produced independently of carbon source at the early logarithmic phase of growth. with the presence of ethanol and so2, the specific polymers production was greater. the polysaccharides comprised of glucose, fructose and galactose, with predominant linkages of alpha-1,4- and alpha-1,6-glucosidic with a ratio 1:1. they contained approximately 5500 hexose molecu ... | 1995 | 8579981 |
microbiology of mesu, a traditional fermented bamboo shoot product. | the use of mesu as a pickle and as the base of curry is a tradition in the darjeeling hills and sikkim of india. a total of 327 strains of lactic acid bacteria, representing lactobacillus plantarum, l. brevis and pediococcus pentosaceus were isolated from 30 samples of mesu. these species were present in all samples of raw bamboo shoots tested. mesu was dominated by l. plantarum followed by l. brevis; p. pentosaceus was isolated less frequently and recovered from only 40-50% of the mesu samples. ... | 1996 | 8722186 |
the effect of cations on the hydrolysis of lactose and the transferase reactions catalysed by beta-galactosidase from six strains of lactic acid bacteria. | beta-galactosidases from lactobacillus delbruekii subsp. bulgaricus 20056, lb. casei 20094, lactococcus lactis subsp. lactis 7962, streptococcus thermophilus ts2, pediococcus pentosaceus pe39 and bifidobacterium bifidum 1901 were partially purified. the rate of hydrolysis of lactose given by the predominant beta-galactosidase activity from each of the bacteria studied was in all cases enhanced by mg2+, while the effect of k+ and na+ differed from strain to strain. the beta-galactosidases from al ... | 1996 | 8987531 |
microflora of murcha: an amylolytic fermentation starter. | murcha is a traditional starter, used commonly in darjeeling hills and sikkim in india to ferment a variety of starchy substrates in order to produce sweet-sour alcoholic beverages, called jnards. murcha cakes are mildly acidic (ph 5.2) and contain 13% w/w moisture and 0.7% w/w ash (dry weight basis). a total of 194 bacterial, 190 yeast and 80 mould strains were isolated from 30 samples of murcha. the counts (cfu/g fresh weight) of micro-organisms in the samples were 2.0 x 10(7) to 4.2 x 10(8) f ... | 1995 | 7476555 |
effect of pediococcus pentosaceus fbb61, pediocin a producer strain, in caecal fermentations. | the objective of this study was to investigate the effect of pediocin a in in vitro caecal fermentations. pediococcus pentosaceus fbb61, pediocin a producer (bac+) and its isogenic mutant (bac-) ped. pentosaceus fbb61-2 were added to fermentation vessels. pediocin a did not alter the normal activity of caecal microflora. nevertheless, the presence of pediocin a producer strain reduced proteolysis compared to the mutant strain as indicated by ammonia concentrations (p < 0.05), and isobutyric and ... | 1995 | 7615417 |
identification of a cold shock gene in lactic acid bacteria and the effect of cold shock on cryotolerance. | when lactic acid bacterial cultures were frozen at -20 degrees c for 24 h, the cell viability decreased drastically, but when they were cold shocked at 10 degrees c for 2 h prior to freezing, viability improved significantly for the lactococcus lactis subsp. lactis strains (25-37%) and pediococcus pentosaceus po2 (18%), but not for the lactococcus lactis subsp. cremoris strains tested or for one strain of lactobacillus helveticus lb1 and streptococcus thermophilus ts2. when the period for cold s ... | 1997 | 9175562 |
identification of pediococcus acidilactici and pediococcus pentosaceus based on 16s rrna and ldhd gene-targeted multiplex pcr analysis. | | 1997 | 9228758 |
molecular characterization of the replicon of the pediococcus pentosaceus 43200 pediocin a plasmid pmd136. | the pediocin a-encoding plasmid of pediococcus pentosaceus 43200, pmd136, was characterized by restriction enzyme analysis. analysis of its replicon was facilitated by the construction of a probe vector consisting of the escherichia coli plasmid psp72 and the cat gene from staphylococcus aureus plasmid pc194. the replication region of pmd136 was localized on a 1.6-kb ecori/bglii fragment. sequencing analysis revealed a non-coding region, repa, spanning the first 440 bp, followed by an open readi ... | 1997 | 9228759 |
expression of glr (muri, dga) gene encoding glutamate racemase in escherichia coli. | the muri (dga) gene of escherichia coli is required for the biosynthesis of d-glutamate, an essential component of bacterial peptidoglycan (doublet, p., van heijetnoort, j., and mengin-lecreulx, d. (1992) j. bacteriol. 174, 5772-5779; dougherty, t. j., thanassi, j. a., and pucci, m. j. (1993) j. bacteriol. 175, 111-116), but its gene product has not been identified. we found that the amino acid sequence of protein deduced from the nucleotide sequence of the open reading frame of muri gene (orf1) ... | 1993 | 7901215 |
the escherichia coli dga (muri) protein shares biological activity and structural domains with the pediococcus pentosaceus glutamate racemase. | the pediococcus pentosaceus glutamate racemase gene product complemented the d-glutamate auxotrophy of escherichia coli wm335. amino acid sequence analysis of the two proteins revealed 28% identity, primarily in six clusters scattered throughout the sequence. further analyses indicated secondary structure similarities between the two proteins. these data support a recent report that the dga (muri) gene product is a glutamate racemase. | 1994 | 7904596 |
isolation and identification of tetracycline resistant lactic acid bacteria from pre-packed sliced meat products. | in recent years, the food chain has been recognised as one of the main routes for transmission of antibiotic resistant bacteria between the animal and human population. in this regard, the current study aimed to investigate if tetracycline resistant (tetr) lactic acid bacteria (lab) are present in ready-to-eat modified atmosphere packed (map) sliced meat products including fermented dry sausage, cooked chicken breast meat and cooked ham. from population graphs based on doubling tetracycline conc ... | 2000 | 10930081 |
pediocin pd-1, a bactericidal antimicrobial peptide from pediococcus damnosus ncfb 1832. | pediocin pd-1, produced by pediococcus damnosus ncfb 1832, is inhibitory to several food spoilage bacteria and food-borne pathogens. however, pediocin pd-1 is not active against other pediococcus spp. and differs in this respect to other pediocins produced by pediococcus acidilactici and pediococcus pentosaceus. production of pediocin pd-1 starts during early growth and reaches-a plateau at the end of exponential growth. pediocin pd-1 was partially purified and its size was determined by tricine ... | 1997 | 9246779 |
inducible metabolism of phenolic acids in pediococcus pentosaceus is encoded by an autoregulated operon which involves a new class of negative transcriptional regulator. | pediococcus pentosaceus displays a substrate-inducible phenolic acid decarboxylase (pad) activity on p-coumaric acid. based on dna sequence homologies between the three pads previously cloned, a dna probe of the lactobacillus plantarum pdc gene was used to screen a p. pentosaceus genomic library in order to clone the corresponding gene of this bacteria. one clone detected with this probe displayed a low pad activity. subcloning of this plasmid insertion allowed us to determine the part of the in ... | 2000 | 11073918 |
expression in escherichia coli of native and chimeric phenolic acid decarboxylases with modified enzymatic activities and method for screening recombinant e. coli strains expressing these enzymes. | four bacterial phenolic acid decarboxylases (pad) from lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis, and bacillus pumilus were expressed in escherichia coli, and their activities on p-coumaric, ferulic, and caffeic acids were compared. although these four enzymes displayed 61% amino acid sequence identity, they exhibit different activities for ferulic and caffeic acid metabolism. to elucidate the domain(s) that determines these differences, chimeric pad proteins were const ... | 2001 | 11229892 |
cloning and expression of the glutamate racemase gene of bacillus pumilus. | a glutamate racemase gene (muri) was found in bacillus pumilus cells and cloned into escherichia coli wm335, a d-glutamate auxotroph, by means of a genetic complement method. muri of b. pumilus encodes a 272-amino acid protein with an unusual initiation codon, ttg. the deduced amino acid sequence shows significant similarity with those of glutamate racemases from e. coli (ratio of identical residues, 28%), pediococcus pentosaceus (44%), and staphylococcus haemolyticus (49%). b. pumilus muri was ... | 1997 | 9354391 |
monoclonal antibody-based enzyme immunoassay for pediocins of pediococcus acidilactici. | monoclonal antibody (mab) r2-ar against pediocin rs2 was developed. mice were immunized for 12 weeks with pediocin rs2 conjugated to a polyacrylamide gel. two hybridoma fusions yielded an mab that in western blots (immunoblots) reacted only with pediocins rs2 and ach (3 kda) from pediococcus acidilactici rs2 and h, respectively, and did not react with any other bacteriocin, including sakacin a from lactobacillus sake lb 706, leuconocin lcm1 from leuconostoc carnosum lm1, nisin from lactococcus l ... | 1994 | 8085814 |
purification and partial characterization of a tripeptidase from pediococcus pentosaceus k9.2. | a tripeptidase was purified from the cytoplasm of pediococcus pentosaceus k9.2 by anion-exchange chromatography, gel filtration chromatography, and high-performance liquid chromatography. the molecular mass of the enzyme was estimated by gel filtration at 100,000 da. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified peptidase showed one protein band of 45,000 da. optimal enzyme activity was obtained at ph 7.0 and at 50 degrees c. the peptidase hydrolyzed all tripeptides t ... | 1997 | 9406407 |
pediocin n5p from pediococcus pentosaceus: adsorption on bacterial strains. | pediocin n5p is a bacteriocin produced by pediococcus pentosaceus isolated from wine. it is absorbed on both sensitive and resistant gram-positive and gram-negative bacteria in non specific (non lethal) sites and higher values, up to 30% are observed in sensitive strains suggesting the presence of particular (lethal) receptors. cell death without lysis is produced by the action of pediocin on sensitive strains. the cations mg2+ and mn2+ increase the pediocin binding by 80 and 100%, respectively. ... | 1998 | 9562879 |
fermentation of fructans by epiphytic lactic acid bacteria. | a total of 712 strains of lactic acid bacteria isolated from forage grasses were studied for their ability to ferment fructans of phlein- as well as inulin-type. only 16 strains utilized phlein and eight of these also fermented inulin. they were identified as lactobacillus paracasei subsp. paracasei, lact. plantarum, lact. brevis and pediococcus pentosaceus. in the species lact. paracasei subsp. paracasei, all strains gave positive results, whereas the other positive strains possessed unique pro ... | 1994 | 8200866 |
construction of a food-grade multiple-copy integration system for lactococcus lactis. | a food-grade vector system was developed that allows stable integration of multiple plasmid copies in the chromosome of lactococcus lactis. the vector consists of the plus origin of replication (ori+) of the lactococcal plasmid pwv01, the sucrose genes of the lactic acid bacterium pediococcus pentosaceus ppe1.0 as selectable marker, a multiple-cloning site, and a lactococcal dna fragment of a well-characterized chromosomal region. the system includes two l. lactis strains, ll108 and ll302, which ... | 1998 | 9615484 |
characterization and frequency distribution of species of lactic acid bacteria involved in the processing of mawè, a fermented maize dough from benin. | lactic acid bacteria involved in the natural fermentation of both home-produced and commercial mawè were investigated during a 72 h fermentation period. lactobacillus spp. constitute the majority (94%) of the strains of the lactic acid bacteria isolated, among which 89% represent the betabacterium group. they include l. fermentum (biotype cellobiosus) (41%), l. fermentum or l. reuteri (19%), l. brevis (26%), l. confusus (less than 2%), l. curvatus (less than 1%) and l. buchneri (less than 1%). o ... | 1993 | 8347427 |
production and characterization of antibacterial compounds produced by pediococcus damnosus and pediococcus pentosaceus. | the broad-spectrum antibacterial activity exhibited by three pediococcus strains isolated from beer was preliminarily characterized. factors affecting the production rate of bacterial inhibitors were screened and the effects of simultaneous cultivation of lactococcus and pediococcus on the production of inhibitory substances were studied. the antibacterial activity against a range of gram-negative test organisms was not affected by catalase or proteolytic enzymes and was extremely thermotolerant ... | 1993 | 8444642 |
characterization of bacteriocin produced by pediococcus pentosaceus from wine. | twenty strains of pediococcus pentosaceus isolated from wine were examined for production of bacteriocins. only two of them showed inhibitory activity, ped. pentosaceus n4p against the indicator strains of the same species and n5p against 19 strains of the three genera of lactic acid bacteria from wine. the antimicrobial substance from n5p strains was removed by membrane (0.2 micron) filtration, destroyed by organic solvents and proteolytic enzymes. it was stable for 60 min at 100 degrees c. the ... | 1993 | 8486547 |
nutritional quality of lactic fermented bitter gourd and fenugreek leaves. | pediococcus pentosaceus was selected from isolates obtained from the naturally fermenting bitter gourd and fenugreek leaves based on its high titre and broad spectrum of inhibitory activity against spoilage organisms. this strain was then employed for fermentation of bitter gourd and fenugreek which resulted in a more acceptable product having enhanced fat, pyridoxine and ascorbic acid levels. it was of interest to note that vitamin b12 was formed in the fenugreek as a result of the fermentation ... | 1998 | 9713580 |
protein modification by fermentation: effect of fermentation on the potential allergenicity of pea. | the effect of fermentation on components of potential significance for the allergenicity of pea was analyzed. pea flour was fermented with three lactic acid bacteria, pediococcus pentosaceus, lactococcus raffinolactis, and lactobacillus plantarum, and two fungi, rhizopus microsporus, var. oligosporus and geotrichum candidum. residual antigenicity against antipea antibodies was reduced to 10% by the three lactic acid bacteria and r. microsporus. reactions to anti-pea profilin and anti-bet v 1 wer ... | 1998 | 9826013 |
study of the micro-organisms associated with the fermented bread (khamir) produced from sorghum in gizan region, saudi arabia. | traditional bread (khamir) was made from sorghum flour of two local varieties, bayadh and hamra. the bread was prepared by mixing the sorghum flour with water and spices (onion, garlic, lemon juice and fenugreek) in a 1:0.8 (w/w) ratio and fermented for 24 h at 30 degrees c. two other fermentations were carried out using an inoculum from the previous fermentation. the micro-organisms were isolated from different plates and identified using different characterization systems. both total bacterial ... | 1999 | 10063620 |
proinflammatory effect of pediococcus pentosaceus, a bacterium used as hay preservative. | bacterial cultures, such as pediococcus pentosaceus, are used to treat hay with the objective of preventing hay heating and moulding, and thus, the development of the microbial growth which causes farmer's lung. the aim of this study was to investigate whether such bacterial cultures have the potential to induce a pulmonary inflammatory response. mice were instilled 3 days week-1 for 3 weeks with either saline or nonviable preparations of p. pentosaceus, saccharopolyspora rectivirgula, lactococc ... | 1996 | 8980961 |
attachment of lactic acid bacteria to beef-muscle surfaces. | the effect of immersion time and cell concentration in the attachment of several lactic acid bacteria with antibacterial activity to beef-muscle surface was studied. the number of firmly attached bacteria increased with immersion time in the case of pediococcus acidilacti, lactobacillus sake, lactococcus cremoris (two strains) and pediococcus acidilacti. pediococcus pentosaceus, lactococcus lactis and lactobacillus curvatus reached maximum adhesion after 15-30 min. the highest strength of attach ... | 1996 | 9131790 |
nucleotide sequence and analysis of plasmid pmd136 from pediococcus pentosaceus fbb61 (atcc43200) involved in pediocin a production. | the complete sequence of the 19515-bp plasmid pmd136 from pediococcus pentosaceus fbb61 (atcc43200) has been determined. this plasmid is involved in pediocin a production, a bacteriocin active against a wide range of gram-positive bacteria. it appears to replicate via a theta mechanism, with structures closely related to those of many lactococcal plasmids. genes homologous to mobilization functions are also present, which are similar in sequence and arrangement to moba, mobb, and mobc of some st ... | 2000 | 10686129 |
characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented weaning food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting. | the group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known, and these organisms have been characterized extensively by using different techniques. in this study, 180 lactic acid bacterial strains isolated from sorghum powder (44 strains) and from corresponding fermented (93 strains) and cooked fermented (43 strains) porridge samples that were prepared in 15 households were characterized by using biochemical and physiological methods, as well as by analyz ... | 2000 | 10698775 |
effect of some nutritional and environmental parameters on the production of diacetyl and on starch consumption by pediococcus pentosaceus and lactobacillus acidophilus in submerged cultures. | three series of 5-day submerged cultures with pediococcus pentosaceus mitj-10 and lactobacillus acidophilus hansen 1748 were carried out in starch-based media, and the effect of cultural factors on the changes of starch, diacetyl and amylase activity determined. in axenic cultures, ped. pentosaceus mitj-10 produced more diacetyl (63.27 mg l(-1)) by adding glucose, yeast extract and caco3 (p < 0.01), at 28 degrees c (p < 0.05); but more starch was consumed (18.4 g l(-1)) in the absence of glucose ... | 2000 | 10735253 |
broad and complex antifungal activity among environmental isolates of lactic acid bacteria. | more than 1200 isolates of lactic acid bacteria isolated from different environments were screened for antifungal activity in a dual-culture agar plate assay. approximately 10% of the isolates showed inhibitory activity and 4% showed strong activity against the indicator mould aspergillus fumigatus. the antifungal spectra for 37 isolates with strong activity and five isolates with low or no activity were determined. several of the strains showed strong inhibitory activity against the moulds a. f ... | 2003 | 12594034 |
enterocin 012, a bacteriocin produced by enterococcus gallinarum isolated from the intestinal tract of ostrich. | enterococcus gallinarum strain 012, isolated from the duodenum of ostrich, produced enterocin 012 which is active against ent. faecalis, lactobacillus acidophilus, lact. sake, listeria innocua, propionibacterium acidipropionici, propionibacterium sp., clostridium perfringens, pseudomonas aeruginosa and salmonella typhimurium. one of the four pathogenic strains of escherichia coli isolated from the intestinal tract of ostrich was inhibited by enterocin 012. no antimicrobial activity was recorded ... | 2000 | 10736005 |
analysis of the genetic determinant for production of the pediocin p of pediococcus pentosaceus pep1. | pediococcus pentosaceus pep1 is a vacuum-packaged turkish sausage isolate which produces a potentially novel bacteriocin of the pediocin (anti-listeria) family of peptides designated as pediocin p. curing experiments and plasmid profile analysis indicated that both bacteriocin immunity and production determinants were linked and encoded by 9.0 mda plasmid, phd1.0. attempts to transform purified plasmid phd1.0 into recipient escherichia coli jm109 cells by electroporation were successful but none ... | 2000 | 10986669 |
[molecular structure of the locus for sucrose utilization by lactobacillus plantarum: comparison with pediococcus pentosaceus]. | structure of the sucrose utilization locus in a lactobacillus plantarum type strain was studied using pcr and southern hybridization. restriction map analysis revealed its high similarity to the sequenced sucrose utilization locus of pediococcus pentosaceus psrq1. the l. plantarum locus proved containing oppositely oriented scra and the scrbragl operon, but not agas. the l. plantarum sucrase gene (scrb) was partly sequenced. a higher (98.6%) homology was revealed between scrb than between the 16 ... | 2001 | 11234378 |
dry sausage fermented by lactobacillus rhamnosus strains. | the ability of three probiotic lactobacillus rhamnosus strains gg, e-97800 and lc-705 and one commercial pediococcus pentosaceus starter strain (control) to produce dry sausage was studied. during the fermentation process the numbers of inoculated lactic acid bacteria increased from approx. 7 log10 to 8-9 log10 cfu/g and the ph values decreased from 5.6 to 4.9-5.0. the sensory test indicated that the dry sausages fermented by l. rhamnosus lc-705 were inferior to the control sausages. the presenc ... | 2001 | 11252505 |
phenotypic and genotypic characterization of pediococcus strains isolated from human clinical sources. | seventy-two strains of pediococci isolated from human clinical sources were characterized by conventional physiological tests, chromogenic enzymatic tests, analysis of whole-cell protein profiles (wcpp) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and analysis of chromosomal dna restriction profiles by pulsed-field gel electrophoresis (pfge). conventional tests allowed identification of 67 isolates: 52 strains were identified as pediococcus acidilactici, 15 strains were identifi ... | 2001 | 11283035 |
detection of lactobacillus, pediococcus, leuconostoc, and weissella species in human feces by using group-specific pcr primers and denaturing gradient gel electrophoresis. | denaturing gradient gel electrophoresis (dgge) of dna fragments generated by pcr with 16s ribosomal dna-targeted group-specific primers was used to detect lactic acid bacteria (lab) of the genera lactobacillus, pediococcus, leuconostoc, and weissella in human feces. analysis of fecal samples of four subjects revealed individual profiles of dna fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria s ... | 2001 | 11375166 |
the effect of lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. | whole-plant barley (39.4% dry matter) was treated with various chemical and biological additives to assess their effects on silage fermentation and aerobic stability. treatments were untreated forage, forage treated with several amounts of lactobacillus buchneri and enzymes (l. buchneri at 1 x 10(5), 5 x 10(5), and 1 x 10(6) cfu/g of fresh forage), forage treated with an inoculant containing (lactobacillus plantarum, pediococcus pentosaceus, propionibacterium freudenreichii, and enzymes), or for ... | 2001 | 11384041 |
[biological deacidification of wines using lactic-acid bacteria and yeasts]. | based on a study of 200 lactic-acid bacteria monocultures and 30 associating lactic bacteria and yeasts cultures, a stable association was created formed by leuconostoc oenos, pediococcus pentosaceus and saccharomyces cerevisiae yeasts, intended for the biological deacidification of wine. physiology of microorganisms and their effect on the wine chemical composition was studied. by means of selective association, high quality fine wines were produced from the high-acid wines. | 2001 | 11530675 |
the gram-positive tonsillar and nasal flora of piglets before and after weaning. | to investigate gram-positive nasal and tonsillar microbial flora of piglets before and after weaning. | 2001 | 11851806 |
growth and aroma production by staphylococcus xylosus, s. carnosus and s. equorum--a comparative study in model systems. | a laboratory medium inoculated with 20 different staphylococcus strains was prepared in accordance with a full factorial experimental design investigating the effect of temperature, ph, nacl and glucose on growth. the 12 strains most suited to growth in a fermented meat environment were inoculated in sausage minces together with pediococcus pentosaceus, incubated at 25 degrees c for 1 week and the produced aroma compounds collected. the data were analysed by multiple linear regression and partia ... | 2002 | 11999121 |
airborne microflora in quebec dairy farms: lack of effect of bacterial hay preservatives. | pediococcus pentosaceus is a lactic-acid producing bacterium inoculated in hay to prevent hay deterioration. this study sought to verify the effect of this treatment on the barn microenvironment. air samples were obtained from 19 barns using bacterial hay treatment and from 18 control barns with six-stage andersen samplers and all-glass impingers. appropriate culture media were used for the recovery and identification of microorganisms. endotoxins were measured with chromogenic limulus amoebocyt ... | 1999 | 10028620 |
the effect of temperature on the growth and persistence of salmonella in fermented liquid pig feed. | two studies were conducted to investigate the effect of temperature on the fate of salmonella enterica subsp. enterica serovar typhimurium dt104:30 in fermented liquid pig feed. these were (1) by co-inoculation of feed with s. typhimurium dt104:30 and pediococcus pentosaceus, as the fermenting organism, and (2) by fermenting feed for 48, 72 or 96 h prior to inoculation with s. typhimurium dt104:30. in co-inoculated feed incubated at 20 degrees c, s. typhimurium dt104:30 persisted for at least 72 ... | 2002 | 12382689 |
characterization and identification of pediococcus species isolated from forage crops and their application for silage preparation. | pediococcus species isolated from forage crops were characterized, and their application to silage preparation was studied. most isolates were distributed on forage crops at low frequency. these isolates could be divided into three (a, b, and c) groups by their sugar fermentation patterns. strains la 3, la 35, and ls 5 are representative isolates from groups a, b, and c, respectively. strains la 3 and la 35 had intragroup dna homology values above 93.6%, showing that they belong to the species p ... | 1999 | 10388681 |
chemical composition of the cell wall of lactic acid bacteria and related species. | in order to examine the relationship between biological activities and the cell wall content, the murein type and the teichoic acid of the cell wall from five strains of bacteria were studied. two of these lactobacillus casei crl 431 and l. acidophilus crl 730, are used in a commercial fermented milk (bio milk), which is believed to be beneficial for health. the other strains, lactococcus lactis crl 526, pediococcus pentosaceus crl 923 and propionibacterium acidipropionici crl 1198 were included ... | 1996 | 8996856 |
cloning, deletion, and characterization of padr, the transcriptional repressor of the phenolic acid decarboxylase-encoding pada gene of lactobacillus plantarum. | lactobacillus plantarum displays a substrate-inducible pada gene encoding a phenolic acid decarboxylase enzyme (pada) that is considered a specific chemical stress response to the inducing substrate. the putative regulator of pada was located in the pada locus based on its 52% identity with padr, the pada gene transcriptional regulator of pediococcus pentosaceus (l. barthelmebs, b. lecomte, c. diviès, and j.-f. cavin, j. bacteriol. 182:6724-6731, 2000). deletion of the l. plantarum padr gene cle ... | 2004 | 15066807 |
exopolysaccharide-producing lactic acid bacteria strains from traditional thai fermented foods: isolation, identification and exopolysaccharide characterization. | lactic acid bacteria (lab) isolated from various traditional thai fermented foods were screened for exopolysaccharides (eps) production. from 104 isolates, two rod-shaped and five coccal-shaped lab were able to produce eps from sucrose on solid media. however, only the cocci were capable of producing eps in liquid media and these were identified as pediococcus pentosaceus. pediococcus pentosaceus strains ap-1 and ap-3 produced eps in high yield. in liquid media containing sucrose as carbon sourc ... | 1999 | 10574086 |
phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis. | a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ... | 2003 | 12514026 |
bacteriocins from pediococcus pentosaceus l and s from pork meat. | two strains of pediococcus pentosaceus were isolated from refrigerated pork and found to produce antimicrobial substances that may inhibit foodborne pathogens and have potential as natural food preservatives. they were named p. pentosaceus l and s. the antimicrobial substances were purified to electrophoretical homogeneity by chloroform extraction and designated pentocins l and s with molecular masses (m) of 27 and 25 kda, respectively. both pentocins also had broad inhibition spectra and were t ... | 2003 | 12568574 |
use of starter cultures of lactic acid bacteria and yeasts in the preparation of togwa, a tanzanian fermented food. | starter cultures of lactic acid bacteria (lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum and pediococcus pentosaceus) and yeasts (candida pelliculosa, candida tropicalis, issatchenkia orientalis and saccharomyes cerevisiae) isolated from native togwa were tested singly or in combination for their ability to ferment maize-sorghum gruel to produce togwa. all species of bacteria showed an ability to ferment the gruel as judged by lowering the ph fr ... | 2003 | 12745235 |
proteolytic activities in togwa, a tanzanian fermented food. | proteolytic activities were investigated in sorghum-based togwa prepared by natural fermentation and using starter cultures previously isolated from the native product, i.e., lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum, pediococcus pentosaceus, and issatchenkia orientalis in coculture with either l. brevis or l. plantarum. both proteinase and aminopeptidase activities were substantially higher in naturally fermented togwa than in those with s ... | 2003 | 12781948 |
potential osmoprotectants for the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila. | the physiological responses of the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila (formely known as p. halophila), subjected to osmotic stress in the presence of molecules known to act as osmoprotectants for other bacteria were studied. in a defined medium, glycine betaine, dimethylsulfonioacetate, choline, proline and l-carnitine were able to relieve inhibition of growth at 0.8 m nacl. the five compounds were shown to efficiently compete with glycine betaine transpor ... | 2003 | 12781949 |
characterization of lactic acid bacteria isolated from a thai low-salt fermented fish product and the role of garlic as substrate for fermentation. | lactic acid bacteria (lab) isolated from raw materials (fish, rice, garlic and banana leaves) and processed som-fak (a thai low-salt fermented fish product) were characterized by api 50-ch and other phenotypic criteria. lactococcus lactis subsp. lactis and leuconostoc citreum were specifically associated with fish fillet and minced fish, lactobacillus paracasei subsp. paracasei with boiled rice and weisella confusa with garlic mix and banana leaves. in addition, lactobacillus plantarum, lactobac ... | 1999 | 10100902 |
optimum production, stability, partial purification and inhibitory spectrum of antimicrobial compounds produced by pediococcus pentosaceus di. | the optimum conditions for production of antimicrobial compounds by pediococcus pentosaceus di were studied. the antimicrobial compounds in cell free fermented medium were extracted (methanol-acetone), then tested for inhibitory activity against fourteen bacterial strains and its stability to heating, various ph and proteolytic enzymes. in addition, methanol-acetone extract was partial purified through sephadex g-100 column and thin-layer chromatography. production of antimicrobial compounds wer ... | 2001 | 11379284 |
fermentation and microflora of plaa-som, a thai fermented fish product prepared with different salt concentrations. | plaa-som is a thai fermented fish product prepared from snakehead fish, salt, palm syrup and sometimes roasted rice. we studied the effects of different salt concentrations on decrease in ph and on microflora composition during fermentation. two low-salt batches were prepared, containing 6% and 7% salt (w/w) as well as two high-salt batches, containing 9% and 11% salt. ph decreased rapidly from 6 to 4.5 in low-salt batches, whereas in high-salt batches, a slow or no decrease in ph was found. lac ... | 2002 | 11883675 |
persistence of escherichia coli o157:h7 in barley silage: effect of a bacterial inoculant. | the effect of a lactic acid producing bacterial (lab) inoculant on the elimination of escherichia coli o157:h7 from barley forage was assessed. | 2002 | 12147077 |
a pcr-based method for identification of lactobacilli at the genus level. | we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ... | 2002 | 12351242 |
microbiological and fermentation characteristics of togwa, a tanzanian fermented food. | selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ... | 2003 | 12423921 |
purification and genetic characterization of plantaricin nc8, a novel coculture-inducible two-peptide bacteriocin from lactobacillus plantarum nc8. | a new, coculture-inducible two-peptide bacteriocin named plantaricin nc8 (plnc8) was isolated from lactobacillus plantarum nc8 cultures which had been induced with lactococcus lactis mg1363 or pediococcus pentosaceus fbb63. this bacteriocin consists of two distinct peptides, named alpha and beta, which were separated by c(2)-c(18) reverse-phase chromatography and whose complementary action is necessary for full plantaricin nc8 activity. n-terminal sequencing of both purified peptides showed 28 a ... | 2003 | 12514019 |
autolytic activity and pediocin-induced lysis in pediococcus acidilactici and pediococcus pentosaceus strains. | to evaluate the autolytic phenotype of pediococcus acidilactici and p. pentosaceus, the peptidoglycan hydrolases content and the effect of pediocin ach/pa-1 and autolysins on cell lysis. | 2003 | 12631191 |
oxidative metabolism in pediococcus pentosaceus. i. role of oxygen and catalase. | dobrogosz, walter j. (pennsylvania state university, university park) and robert w. stone. oxidative metabolism in pediococcus pentosaceus. i. role of oxygen and catalase. j. bacteriol. 84:716-723. 1962.-studies were conducted on the physiological behavior of several strains of pediococcus pentosaceus isolated from alfalfa silages. although these organisms are regarded as homofermentative lactic acid bacteria which metabolize carbohydrates via the classic reactions of glycolysis, this investigat ... | 1962 | 14028239 |
oxidative metabolism in pediococcus pentosaceus. ii. factors controlling the formation of oxidative activities. | dobrogosz, walter j. (the pennsylvania state university, university park) and robert w. stone. oxidative metabolism in pediococcus pentosaceus. ii. factors controlling the formation of oxidative activities. j. bacteriol. 84:724-729. 1962.-studies were conducted on some factors which regulate the formation of oxidative activities in pediococcus pentosaceus, a homofermentative lactic acid organism. oxidative abilities were defined as the capacities to oxidize glucose and glycerol, and to exhibit c ... | 1962 | 14028240 |
induction and repression of l-arabinose isomerase in pediococcus pentosaceus. | dobrogosz, walter j. (university of illinois, urbana) and ralph d. demoss. induction and repression of l-arabinose isomerase in pediococcus pentosaceus. j. bacteriol. 85:1350-1355. 1963.-the inducible l-arabinose isomerase of pediococcus pentosaceus can be rapidly and conveniently measured in whole-cell preparations by use of a standard colorimetric procedure originally developed for studies with cell-free enzyme preparations. the enzyme is measured by its ability to catalyze the isomerization o ... | 1963 | 14047229 |
pentose utilization by pediococcus pentosaceus. | dobrogosz, walter j. (university of illinois, urbana) and ralph d. demoss. pentose utilization by pediococcus pentosaceus. j. bacteriol. 85:1356-1364. 1963.-data are presented which indicate that pentoses are metabolized by pediococcus pentosaceus through the use of an inducible phosphoketolase pathway. the utilization of each of the pentoses (l-arabinose, d-ribose, and d-xylose) appears to involve a different regulatory process. thus, d-ribose was fermented by cells grown on any pentose or gluc ... | 1963 | 14047230 |
glucose dehydrogenase activity in pediococcus pentosaceus. | | 1963 | 14051811 |
enzyme formation in pediococcus pentosaceus treated with mitomycin c. | | 1963 | 14066495 |
the effects of using lactic acid bacteria inoculants in maize silage on the formation of biogenic amines. | silages from five ripened varieties of silage maize with dry matter contents ranging between 275 and 410 g/kg were prepared in five laboratory experiments. whole-plant maize was fermented at 22 degrees c and silages were then stored at the same temperature for 4 months. spontaneously fermented silages were prepared as control variants and compared with silages inoculated with commercial strains of lactobacillus plantarum, lactobzcillus buchneri and a mixed preparation microsil containing l. plan ... | 2003 | 14620909 |
two distinct pathways for the formation of hydroxy fa from linoleic acid by lactic acid bacteria. | twenty-three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy fa. two distinct conversion pathways were in operation. two strains of lactobacillus acidophilus and a strain of pediococcus pentosaceus produced 13(s)-hydroxy-9-octadecenoic acid 113(s)-oh 18:11 and 10,13dihydroxyoctadecanoic acid (10,13-oh 18:0) as main and minor products, respectively, whereas 13 strains, including l. casei subsp. casei, l. paracasei subsp. paracasei, l. rhamnosus, l. lactis subsp. cremo ... | 2003 | 14870930 |
purification and characterization of bacteriocin from pediococcus pentosaceus accel. | the pediococcus pentosaceus accel bacteriocin was purified to electrophoretical homogeneity by cell adsorption-desorption and superose 12 fast performance liquid chromatography (fplc). the purified bacteriocin, with a molecular mass of 17.5 kda and an n-terminal sequence of -kyygngvtxgkhsxxvdxg-, belongs to class iia and is designated pediocin accel. it was inactivated by various proteases and stable at ph 2.0-6.0 and <100 degrees c. more than 80% activity was left even after 15 min of heating a ... | 2004 | 14995112 |
relative ability of orally administered lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract. | five porcine-derived lactobacillus or pediococcus isolates administered to pigs (n = 4), either singly or as a combination at approximately 10(10) cfu per day varied with respect to intestinal survival and persistence. two lactobacillus murinus strains survived best and were excreted at approximately 10(7) to 10(8) cfu/g of feces. in contrast, pediococcus pentosaceus dpc6006 had the lowest fecal count at approximately 10(5) cfu/g and was excreted at a significantly lower level than both l. murin ... | 2004 | 15066778 |
diversity of lactic acid bacteria isolated from aoc salers cheese. | the objective of this work was to describe the diversity of lactic acid bacteria in traditional raw milk salers cheeses at the species and strain levels. the characterization of 381 strains isolated during ripening and various strain collections was investigated using physiological analysis and molecular techniques: rep-pcr, species and genus specific amplifications and the sequence analysis of 16s rdna for strain typing and taxonomic identification. the strains belonged to lactobacillus plantar ... | 2004 | 15190953 |
characterization of mesentericin st99, a bacteriocin produced by leuconostoc mesenteroides subsp. dextranicum st99 isolated from boza. | lactic acid bacteria isolated from boza, a cereal-fermented beverage from belogratchik, bulgaria, were screened for the production of bacteriocins. with the first screening, 13 of the 52 isolates inhibited the growth of listeria innocua and lactobacillus plantarum. the cell-free supernatant of one of these strains, classified as leuconostoc mesenteroides subsp. dextranicum st99, inhibited the growth of bacillus subtilis, enterococcus faecalis, several lactobacillus spp., lactococcus lactis subsp ... | 2004 | 15252717 |
population dynamics of the constitutive biota of french dry sausages in a pilot-scale ripening chamber. | the population dynamic of constitutive biota on 84 samples belonging to two different types of french fermented dry sausages during the ripening process in a pilot-scale ripening chamber was investigated. samples were analyzed in three steps of their production: fresh product, first drying stage, and finished product. in addition, 180 strains of lactic acid bacteria were identified using a miniaturized biochemical procedure of characterization. in general, the number of lactic acid bacteria that ... | 2004 | 15508649 |
the ph-unrelated influence of salt, temperature and manganese on aroma formation by staphylococcus xylosus and staphylococcus carnosus in a fermented meat model system. | the influence of manganese (0.01-0.1-1.0 microg/g), temperature (15-24 degrees c) and salt (3-4% w/w) on volatile formation in model minces inoculated with pediococcus pentosaceus and either staphylococcus xylosus or staphylococcus carnosus was studied in a full factorial experiment. in order to study the direct, ph-unrelated effect of the parameters, data were analysed by use of multiple linear regression and partial least-squares regression both before and after transformation of the volatile ... | 2004 | 15527916 |