| transport of local anaesthetics across chromatophore membranes. | 1. both simple amines and tertiary amino local anaesthetics give rise to an accelerated decay of the absorption change of added ph indicator dyes and a decelerated decay of the endogenous carotenoid absorption band shift, following short flash excitation of rhodopseudomonas sphaeroides chromatophores. 2. with increasing medium ph, lower concentrations of amine or local anaesthetics are effective. 3. the order of potency of the local anaesthetics concurs with their reported membrane/buffer partit ... | 1979 | 312654 |
| orientation and linear dichroism of the reaction centers from rhodopseudomonas sphaeroides r-26. | linear dicroism of chromatophores and isolated reaction centers from the photosynthetic bacterium rhodopseudomonas sphaeroides strain r-26 was studied using a novel technique of orientation. the results are discussed in view of the reaction center structure and its position in the membrane. the advantages of the new orientation technique are also outlined. | 1979 | 312655 |
| linear dichroism of light harvesting bacteriochlorophyll proteins from rhodopseudomonas sphaeroides in stretched polyvinyl alcohol films. | light-harvesting bacteriochlorophyll-protein complexes from rhodopseudomonas sphaeroides 2.4.1 and r-26 mutant are solubilized in sodium dodecyl sulfate and imbedded in polyvinyl alcohol. stretching induces orientation, and the linear dichroism of visible and near infrared absorption is analyzed. based on a simple model, angles between the particle axis and the transition dipole moments are found. in the near infrared absorption band of the r-26 light-harvesting protein the dichroic ratio varies ... | 1979 | 312656 |
| the orientations of reaction center transition moments in the chromatophore membrane of rhodopseudomonas sphareroides, bases on new linear dichroism and photoselection measurements. | chromatophore membranes from rhodopseudomonas sphaeroides were oriented by drying suspensions on the surfaces of glass slides, polarized spectra of light-induced absorption changes were obtained between 500 and 1000 nm. as observed earlier, these spectra showed negative bands, reflecting photooxidation of the bacteriochlorophyll 'special pair' in the reaction centers, centered near 870, 810, 630 and 600 nm. these bands have been designated by1, by2, bx1 and bx2, respectively, corresponding to tw ... | 1979 | 312657 |
| bacteriocholorophyll a cation radical in solution and in reaction centers of rhodopseudomonas sphaeroides. resonance raman scattering. | resonance raman spectra of the pi-cation of bacterio-chlorophyll a in solution at 30 k are reported and discussed. outer (formula: see text) bonds of the pyrroles and the methine bridges are weakened by the ionization, while (formula: see text) and mg-n bonds remain essentially unaffected. resonance raman spectra of reaction centers suggest that the positive charge on p-870+ should be localized on a single bacteriochlorophyll molecule by the lifetime of the scattering process (approximately 10(- ... | 1979 | 312659 |
| membrane potential changes during chemotaxis of rhodopseudomonas sphaeroides. | | 1979 | 313342 |
| membranes of rhodopseudomonas sphaeroides: effect of cerulenin on assembly of chromatophore membrane. | the effects of cerulenin were investigated in rhodopseudomonas sphaeroides to elucidate further the mechanisms controlling the assembly of the chromatophore membrane. when this potent inhibitor of fatty acid biosynthesis was added to photosynthetically grown cultures, there was an immediate cessation of phospholipid, bacteriochlorophyll a, carotenoid, and ubiquinone formation. concurrently, there was also a marked decrease in the rate of incorporation of protein into the chromatophore membrane. ... | 1979 | 313392 |
| electric field-induced polarization of photosynthetic membranes and reaction centers of rhodopseudomonas sphaeroides, strain 1760-1. | electric fields of 10(5) v/cm cause the polarization of chromatophore and reaction center films prepared from photosynthesizing purple bacteria. photosynthetic pigments, and in particular carotenoids, the absorption spectra of which are changed in response to electric fields (electrochromism), may serve as intrinsic indicators of the development of a polarized state. polarization occurs due to changes in the orientation and the spatial position of different charged groups and particles. the fiel ... | 1978 | 314216 |
| membranes of rhodopseudomonas sphaeroides. vi. isolation of a fraction enriched in newly synthesized bacteriochlorophyll alpha-protein complexes. | radioactivity eventually destined for the chromatophore membrane of rhodopseudomonas sphaeroides was shown in pulse-chase studies to appear first in a distinct pigmented fraction. the material formed an upper pigmented band which sedimented more slowly than chromatophores when cell-free extracts were subjected directly to rate-zone sedimentation on sucrose density gradients. sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the purified fraction contained polypeptide bands ... | 1979 | 314307 |
| glutamine as a feedback inhibitor of the rhodopseudomonas sphaeroides nitrogenase system. | in whole cells of rhodopseudomonas sphaeroides, nitrogen fixation, as measured by hydrogen production and acetylene reduction, was totally inhibited by micromolar concentrations of ammonia. this inhibition could not be duplicated by glutamate or glutamine alone. the inhibition by ammonia was abolished by methionine sulfoximine, a glutamine synthetase inhibitor. inhibition by glutamine was complete in the presence of methionine sulfone, a preferential inhibitor of glutamate synthase, presumably b ... | 1979 | 314444 |
| crossed immunoelectrophoretic analysis of chromatophore membranes from rhodopseudomonas sphaeroides. | triton extracts of intracytoplasmic photosynthetic membranes (chromatophores) purified from rhodopseudomonas sphaeroides were subjected to crossed immunoelectrophoresis with antiserum raised in rabbits to purified chromatophores. a total of 31 immunoprecipitates was visualized; 2 of the immunoprecipitates were identified as reduced nicotinamide adenine dinucleotide (ec 1.6.99.3) and l-lactate dehydrogenases by enzyme staining techniques. reaction with a monospecific antiserum identified the phot ... | 1979 | 314445 |
| on the nature of the activating enzyme of the inactive form of delta-aminolevulinate synthetase in rhodopseudomonas spheroides. | the activating enzyme of the inactive form of fraction i of delta-aminolevulinate (ala) synthetase [ec 2.3.1.37] in rhodopseudomonas (r.) spheroides was purified about 1,000-fold from an extract of r. spheroides cells grown anaerobically in the light. the purification of the activating enzyme was achieved by fractionating the 100,000 x g supernatant fraction of the crude extract with ammonium sulfate and acetone, followed by sephadex g-200 chromatography, pyridoxamine phosphate-sepharose 4b chro ... | 1979 | 314447 |
| occurrence of a novel high molecular weight activator of delta-aminolevulinate synthetase in rhodopseudomonas spheroides. | | 1979 | 314448 |
| the resonance raman spectrum of carotenoids as an intrinsic probe for membrane potential. oscillatory changes in the spectrum of neurosporene in the chromatophores of rhodopseudomonas sphaeroides. | the resonance raman spectrum of the carotenoid neurosporene is shown to be a sensitive monitor of absorption shifts, and thus changes in membrane potential, in chromatophores of the glc mutant of rhodopseudomonas sphaeroides. for a raman excitation wavelength at 472.7 nm, the intensities of the two most prominent resonance raman features (v1 and v2) respond very differently to small shifts in the absorption maxima. thus, the ratio intensity v1/intensity v2 is a sensitive probe for absorption shi ... | 1979 | 314816 |
| [polarization of photosynthetic membranes and reaction centers of rhodopseudomonas sphaeroides 1760-1 in an external electrical field]. | electric fields as high as 10(5) v/cm cause polarization of chromatophores and reaction centre films prepared from photosynthesizing bacteria. photosynthetic pigments, carotenoids in particular, the absorption spectra of which are changed in response to electric fields, may serve as an intrinsic indicator of the development of a polarized state. polarization occurs due to changes in orientation and spacial position of different charge groups and particles. the field-induced polarized state can b ... | 1979 | 314820 |
| energy transduction in membranes of rhodopseudomonas sphaeroides [proceedings]. | | 1979 | 315206 |
| fluorescence yield properties of a fraction enriched in newly synthesized bacteriochlorophyll a-protein complexes from rhodopseudomonas sphaeroides. | | 1979 | 315566 |
| membranes of rhodopseudomonas sphaeroides. vii. photochemical properties of a fraction enriched in newly synthesized bacteriochlorophyll a-protein complexes. | previous pulse-chase studies have shown that bacteriochlorophyll a-protein complexes destined eventually for the photosynthetic (chromatophore) membrane of rhodopseudomonas sphaeroides appear first in a distinct pigmented fraction. this rapidly labeled material forms an upper band when extracts of phototrophically grown cells are subjected directly to rate-zone sedimentation. in the present investigation, flash-induced absorbance changes at 605 nm have demonstrated that the upper fraction is enr ... | 1979 | 315795 |
| structural studies on reconstituted reaction center-phosphatidylcholine membranes. | reaction center protein, isolated from the photosynthetic bacterium rhodopseudomonas sphaeroides r26 mutant, was incorporated into phosphatidylcholine bilayers forming a homogeneous population of unilamellar vesicles. cytochrome c, added to preformed reaction center-phosphatidylcholine vesicles, rapidly reduced up to 90% of the laser-generated (bchl)2+ of the reaction center (with kinetics of electron transfer similar to those in the chromatophore membrane) which suggests that the portion of the ... | 1979 | 315796 |
| the reconstitution of energy transfer in membranes from a bacteriochlorophyll-less mutant of rhodopseudomonas sphaeroides by addition of light-harvesting and reaction centre pigment-protein complexes. | antenna and reaction centre complexes purified from photosynthetically-grown cells of rhodopseudomonas sphaeroides have been mixed with cytoplasmic membranes prepared from an aerobically-grown bacteriochlorophyll-less mutant of rp. sphaeroides (designated 01) in the presence of 1% sodium cholate. after removal of the cholate by dislysis, the dislysate was subjected to isopycnic centrifugation. reconstituted cytochrome c2 photooxidation and cytochrome b photoreduction was demonstrated in a pigmen ... | 1979 | 315797 |
| activation of ribulose 1,5-bisphosphate carboxylase from rhodopseudomonas sphaeroides: probable role of the small subunit. | the activation properties of the form i and form ii ribulose 1,5-bisphosphate carboxylases from rhodopseudomonas sphaeroides were examined. both enzymes have a requirement of mg2+ for optimal activity. mn2+, ni2+, and co2+ can also support activity of the form i enzyme, whereas only mn2+ can substitute for mg2+ with the form ii enzyme. the effect of different preincubations on the carboxylase reaction was also examined. both enzymes exhibited a lag when preincubated with other than mg2+ and co2 ... | 1979 | 316430 |
| bacteriochlorophyll a-types in chromatophore and subchromatophore preparations from rhodopseudomonas sphaeroides. | comparison of absorption and circular dichroism (cd) spectra in the near infrared region was made with chromatophore and subchromatophore preparations obtained from rhodopseudomonas sphaeroides. the 850 nm absorption band had a positive correlation with the 850 nm and 870 nm cd bands. the 800 nm and 870 nm absorption bands seemed not to correlate with any cd bands. lipid contents in chromatophores and subchromatophores were measured. lipids in membranes seemed to contribute to the appearance of ... | 1979 | 316431 |
| photochemical reactions centre of photosynthetic bacteria. | | 1979 | 317049 |
| host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli. | the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ... | 1977 | 329101 |
| formation of cobalt-free corrinoids in rhodopseudomonas spheroides. | co-free corrinoids (cfcs) were found in the cells of rhodopseudomonas spheroides grown without cobalt salt. a predominant fraction of three cfcs on paper-electrophoregram was further purified, and investigated physico-chemically as well as microbiologically in comparison with that isolated by toohey from chromatium. the electrophoretic as well as spectrophotometric patterns of cfc from rh. spheroides were partly different from those of chromatium cfc. the former cfc was a competitive inhibitor o ... | 1977 | 335029 |
| plasmid transfer and expression in rhodopseudomonas sphaeroides. | | 1978 | 350162 |
| nutritional improvement of food proteins by enzymatic modification, especially by plastein synthesis reaction. | the present paper reviews our recents studies on the plastein reaction applied to improve nutritional quality of conventional and unconventional proteins, with special emphasis on the papaincatalyzed incorporation of essential amino acids (used in ethyl ester form) into the proteins : l-methionine into soybean protein, l-lysine into wheat gluten and l-tyrosine into fish protein (after removal of phenylalanine). the paper deals also with an attempt to improve proteins extracted from the photosynt ... | 1978 | 360951 |
| localisation of the subunits of the photosynthetic reaction centers in the chromatophore membrane of rhodospirillum rubrum. | reaction centers were isolated with the detergent lauryl dimethyl amine oxide from chromatophore membranes of rhodospirillum rubrum. the subunit composition of these reaction centers is similar to the one obtained from rhodopseudomonas spheroides: three subunits with the molecular weights of 21 000, 24 000 and 29 000. reaction centers prepared from chromatophores labeled with 131i were heavely labeled in their large subunit (h). the smaller subunits (l and m) contained only little label. sonicat ... | 1977 | 403945 |
| transfer of chromosomal genes mediated by plasmid r68.45 in rhodopseudomonas sphaeroides. | plasmid r68.45 was transferred from pseudomonas aeruginosa pao25 to the photosynthetic species rhodopseudomonas gelatinosa and rhodopseudomonas sphaeroides by selection for resistance to antibiotics. r. sphaeroides strains carrying the plasmid could transfer the plasmid and also chromosomal genes to other strains of r. sphaeroides. | 1977 | 407213 |
| reduction of adenylylsulfate and 3'-phosphoadenylylsulfate in phototrophic bacteria. | extracts of 14 species of phototrophic bacteria, partly grown with different sulfur compounds, were tested for their ability to form volatile sulfur compounds from adenylylsulfate (aps) and 3'-phosphoadenylylsulfate (paps). the rhodospirillum species showed marked activities with both aps and paps while the rhodopseudomonas species seem to prefer paps. the chromatiaceae exhibited the strongest activities with aps, whereas chlorobium limicola had equally high activity with paps. | 1977 | 408177 |
| [carbonic anhydrase activity of phototropic bacteria]. | the activity of carboanhydrase was assayed in seven species of phototrophous bacteria: three species of the rhodospirillaceae genus, three species of the chromatiaceae genus, and one species of the chlorobiaceae genus. the activity of carboanhydrase was found in five species among seven. it decreased on passing from photoautotrophous to photoheterotrophous conditions of growth, and then to dark heterotrophous conditions, which correlated with the activity of the calvin cycle in the bacteria. no ... | 1977 | 408582 |
| structural aspects of the reaction center of photosynthetic bacteria calculated from triplet state zero-field splittings. | | 1977 | 410658 |
| a comparison of electron transport and photophosphorylation systems of rhodopseudomonas capsulata and rhodospirillum rubrum. effects of antimycin a and dibromothymoquinone. | the photophosphorylation systems of rhodopseudomonas capsulata and rhodospirillum rubrum chromatophores have been compared in respect to the effects of artificial electron carriers [n-methylphenazonium methosulfate (pms) and diaminodurene], reducing agents (ascorbate in particular), and various quinones in the absence and presence of the electron transport inhibitors antimycin a and dibromothymoquinone (dbmib). in addition, the effects of both inhibitors on photosynthetic electron transport thro ... | 1978 | 414685 |
| location of o-methyl sugars in antigenic (lipo-)polysaccharides of photosynthetic bacteria and cyanobacteria. | an attempt was made to localize a number of o-methyl sugars in lipopolysaccharides and antigenic polysaccharides isolated from photosynthetic bacteria and from cyano-bacteria. methylation analysis with [2h3]methyl iodide as methylating agent was the method of choice. one has to differentiate between (lipo-)polysaccharides having only trace amounts (less than 1% of polymer dry weight) of o-methyl sugars and those having them in larger amounts (more than 4% of polymer dry weight). in the former ca ... | 1978 | 418776 |
| tetrapyrrole biosynthesis from 4,5-dioxovaleric acid in rhodopseudomonas spheroides. | prophyrin biosynthesis from 4,5-dioxovaleric acid was studied in cell suspensions of r. spheroides. the experiments show that 4,5-dioxovaleric acid is a far precursor of porphyrins through delta amino laevulinic acid formation in a transmination reaction involving also l-alanine. it differs radically from the classical delta aminolaevulinic acid synthesis using glycine and succinyl coa as substrates. | 1979 | 421821 |
| the size and number of intramembrane particles in cells of the photosynthetic bacterium rhodopseudomonas capsulata studied by freeze-fracture electron microscopy. | by freeze-fracture electron microscopy, particles have been observed on the protoplasmic leaflet (pf face) of cytoplasmic and intracytoplasmic membranes of the photosynthetic bacterium rhodopseudomonas capsulata. the particles are present under all culture conditions of chemotrophically and phototrophically grown cells. however, the number of particles per microm2 increased significantly when the formation of the photosynthetic apparatus in the membrane is induced. intracytoplasmic membranes, wh ... | 1979 | 428618 |
| the formation of homospermidine by an enzyme from rhodopseudomonas viridis [proceedings]. | | 1979 | 437275 |
| modifiable chromatophore proteins in photosynthetic bacteria. | the chromatophores of chromatium vinosum, as well as six other photosynthetic bacteria, contained two or more proteins which were insoluble when heated in the presence of sodium dodecyl sulfate (sds) and 2-mercaptoethanol (beta-me). when the chromatophores were dissolved at room temperature in sds-beta-me, these proteins were present in the sds-polyacrylamide gel electrophoresis profiles, but when the samples were dissolved at 100 degrees c, they were absent or considerably diminished. when one- ... | 1979 | 438130 |
| secondary electron transfer in chromatophores of rhodopseudomonas capsulata a1a pho. binary out-of-phase oscillations in ubisemiauinone formation and cytochrome b50 reduction with consective light flashes. | | 1979 | 446736 |
| response of rhodopseudomonas capsulata to illumination and growth rate in a light-limited continuous culture. | rhodopseudomonas capsulata was grown under anaerobic, photosynthetic conditions in a continuous culture device. under light limitation, at a constant dilution rate, it was shown that cell composition, including photopigment (bacteriochlorophyll and carotenoids) and ribonucleic acid content, was not affected by incident light intensity; however, steady state culture density varied directly and linearly with light intensity. on the other hand, photopigment and ribonucleic acid levels were affected ... | 1979 | 457612 |
| [effect of formate on the respiration of different microorganisms]. | the cells of methylotrophic bacteria (achromobacter parvulus 1, pseudomonas methylica 2 and 20, ps. fluorescens 45, ps. oleovorans 52) and candida methylica 101 grown in a medium with methanol take up oxygen in the presence of formiate. a. parvulus is most resistant to formiate (km = 2.3 +/- 0.5) x 10(-3) m; ki = 4 x 10(-1) m). when grown on cn substrates, these microorganisms cannot oxidize formiate (with an exception of a. parvulus). formiate also serves as a respiration substrate for e. coli ... | 1979 | 481274 |
| effects of light intensity on membrane differentiation in rhodopseudomonas capsulata. | cells of rhodopseudomonas capsulata, strain 37b4, leu-, precultivated anaerobically under low light intensity, were exposed to high light intensity (2000 w.m-2). the cells grew with a mass doubling time of 3 h. the synthesis of bacteriochlorophyll (bchl) began after two doublings of cell mass. reaction center and light-harvesting bchl i (b-875) were the main constituents of the photosynthetic apparatus incorporated into the membrane. the size of the photosynthetic unit (total bchl/reaction cente ... | 1979 | 486432 |
| characterization of the gene transfer agent made by an overproducer mutant of rhodopseudomonas capsulata. | | 1979 | 490646 |
| phosphorylation coupled to h2 oxidation by chromatophores from rhodopseudomonas capsulata. | | 1979 | 499500 |
| on the calibration of the carotenoid band shift with diffusion potentials. | | 1979 | 499529 |
| effect of growth condition on enzymes of the citric acid cycle and the glyoxylate cycle in the photosynthetic bacterium rhodopseudomonas palustris. | the enzymes of the citric acid and glyoxylate cycles as well as rubp4 carboxylase were measured in cell-free extracts from rhodopseudomonas palustris after growth under chemoheterotrophic, photoheterotrophic and photolithotrophic conditions. although the citric acid cycle was found to be complete under all growth conditions, significant differences in certain enzyme activities occurred as a function of the different energy sources applied. the glyoxylate cycle also was complete under all growth ... | 1979 | 552814 |
| phospholipid composition of photosynthetic membranes of rhodopseudomonas capsulata. | the phospholipids and the fatty acids present in membranes of cells of rhodopseudomonas capsulata, grown photosynthetically in anaerobiosis, were analyzed by thin layer chromatography and gas chromatography-mass spectrometry. the three phospholipids detected, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol, contained about 80% of a single monounsaturated c18 fatty acid, cis-vaccenic acid. these membranes offer therefore a naturally occurring model system endowed with an ex ... | 1979 | 553902 |
| biological activities of lipopolysaccharides and lipid a from rhodospirillaceae. | the lipopolysaccharides and free lipid a from several strains of rhodospirillaceae were assayed comparatively with those of enterobacteriaceae in a number of biological tests. free lipid a's from rhodopseudomonas gelatinosa and rhodospirillum tenue exhibited strong serological cross-reactions with each other and with free lipid a from salmonella. lipid a's from rhodopseudomonas viridis and rhodopseudomonas palustris, although cross-reacting with each other, did not do so with either the lipid a ... | 1977 | 558961 |
| electrochromic absorbance changes of photosynthetic pigments in rhodopseudomonas sphaeroides. i. stimulation by secondary electron transport at low temperature. | light-induced absorbance changes were measured at temperatures between --30 and --55 degrees c in chromatophores of rhodopseudomonas sphaeroides. absorbance changes due to photooxidation of reaction center bacteriochlorophyll (p-870) were accompanied by a red shift of the absorption bands of a carotenoid. the red shift was inhibited by gramicidin d. the kinetics of p-870 indicated electron transport from the "primary" to a secondary electron acceptor. this electron transport was slowed down by l ... | 1977 | 588564 |
| electrochromic absorbance changes of photosynthetic pigments in rhodopseudomonas sphaeroides. ii. analysis of the band shifts of carotenoid and bacteriochlorophyll. | | 1977 | 588565 |
| light-induced electron transport pathways in membrane preparations from rhodopseudomonas capsulata. | | 1977 | 596882 |
| cyanide reduction by nitrogenase in intact cells of rhodopseudomonas gelatinose molisch. | intact cells of rhodopseudomonas gelatinosa, grown in n-free medium, reduce cyanide to methane and ammonia at a rate 5--8 times lower than acetylene. cyanide reduction in whole cells is distinctly more sensitive to cyanide inhibition compared with enzyme preparations. these results are discussed in view of the exploitation of nitrogen-fixing photobacteria in the anaerobic detoxification of cyanide-containing wastewaters. | 1977 | 602470 |
| differentiation of the intracytoplasmic membrane of rhodopseudomonas palustris induced by variations of oxygen partial pressure or light intensity. | the photosynthetic apparatus of rhodopseudomonas palustris contains, in addition to reaction center bacteriochlorophyll (bchl) two spectral forms of light harvesting (lh) bchl, i.e. lh bchl i, characterized by an infrared absorption maximum at 880 nm (890 nm at 77 degrees k) and lh bchl ii absorbing at 805 and 855 nm (805 and 870 nm at 77 degrees k). lh bchl i seems to be associated with a single protein species of an apparent mol. wt. of 13 000 whereas lh bchl ii is apparently associated with t ... | 1977 | 603338 |
| primary photochemical processes in isolated reaction centers of rhodopseudomonas viridis. | picosecond and nanosecond spectroscopic techniques have been used to study the primary electron transfer processes in reaction centers isolated from the photosynthetic bacterium rhodopseudomonas viridis. following flash excitation, the first excited singlet state (p*) of the bacteriochlorophyll complex (p) transfers an electron to an intermediate acceptor (i) in less than 20 ps. the radical pair state p+i-) subsequently transfers an electron to another acceptor (x) in about 230 ps. there is an a ... | 1978 | 620009 |
| the formation of bacteriochlorophyll.protein complexes of the photosynthetic apparatus of rhodopseudomonas capsulata during early stages of development. | | 1978 | 620011 |
| aerobic nitrogen fixation by rhodopseudomonas capsulata. | | 1978 | 620801 |
| phototrophic purple and green bacteria in a sewage treatment plant. | in all purification stages of a biological sewage treatment plant, phototrophic bacteria were detected by the method of viable cell counts. the predominant species identified belonged to the genus rhodopseudomonas of purple nonsulfur bacteria. the number of phototrophic bacteria was highest in wastewater containing sludge. in activated sludge, an average of 10(5) viable cells/ml was found; the number depended upon concentration of sludge rather than on seasonal changes in light conditions in the ... | 1978 | 623470 |
| direct measurement of pure absorbance spectra of living phototrophic microorganisms. | the pure absorbance of turbid cell suspensions of various phototrophic microorganisms were determined by collecting the scattered light. a conventional spectrophotometer was used, equipped with an intergrating sphere as receiver unit, which allowed precise measurements of the absorbance in the range from zero to 0.1. in the wavelength range 300--1100 nm, where photosynthesis occurs, light scattered only once by a bacterial cell retains predominantly the forward direction. this allows measurement ... | 1978 | 626755 |
| properties of photochemical reaction centers purified from rhodopseudomonas gelatinosa. | reaction centers were isolated from a carotenoidless mutant of rhodopseudomonas gelatinosa by hydroxyapatite chromatography of purified chromatophores treated with lauryl dimethyl amine oxide. absorption spectra and spectra of light-induced absorbance changes are similar to those of reaction centers from rhodopseudomonas sphaeroides. the ratio of absorbance at 280 nm to that at 799 nm was 1.8 in the purest preparations. the extinction coefficient at the 799 nm absorption maximum was estimated to ... | 1978 | 629960 |
| molar extinction coefficients and other properties of an improved reaction center preparation from rhodopseudomonas viridis. | | 1978 | 629961 |
| isolation and characterization of light harvesting bacteriochlorophyll.protein complexes from rhodopseudomonas capsulata. | the isolation of two native light harvesting bacteriochlorophyl.protein complexes from rhodopseudomonas capsulata is described. the light harvesting bacteriochlorophyll i (b 875) has been isolated from the blue-green mutant a1a+ lacking both carotenoids and light harvesting bacteriochlorophyll ii. light harvesting bacteriochlorophyll i is associated with a protein (light harvesting band 2) of 12 000 molecular weight. light harvesting bacteriochlorophyll ii complex has been isolated from the muta ... | 1978 | 629962 |
| activation and inactivation of citrate lyase ligase from rhodopseudomonas gelatinosa. | | 1978 | 639994 |
| interrelationships of isoacceptor phenylalanine trna species of rhodopseudomonas sphaeroides. | the phenylalanine trna of rhodopseudomonas sphaeroides was fractionated on benzoylated diethylaminoethyl-cellulose into four isoaccepting species (trnaphei to iv). trnapheiii represented 80% of the total trnaphe in anaerobic, photosynthetically grown organisms, whereas in cultures grown aerobically for prolonged periods, trnapheii represented 80% of the total. in cultures adapting to aerobic growth, the addition of rifampin resulted in a trnaphe profile characteristic of anaerobic-photosynthetic ... | 1978 | 641006 |
| potassium transport system of rhodopseudomonas capsulata. | rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. these cations were actively accumulated by the cells by a process following michaelis-menten saturation kinetics. the monovalent cation transport system had km's of 0.2 mm k+, 0.5 mm rb+, and 2.6 mm cs+. the rates of uptake of substrates by the potassium transport system varied with the age of the culture, although the affinity constant for the substrates remained constant. the maximal veloc ... | 1978 | 641010 |
| divalent cation transport systems of rhodopseudomonas capsulata. | separate divalent cation transport systems for energy-dependent uptake of mg2+ and mn2+ were found both with aerobically and heterotrophically grown and with photosynthetically grown cells of rhodopseudomonas capsulata. the maximum rate of mg2+ uptake differed between photosynthetic and aerobic cells, while the km for the mg2+ transport system was constant. photosynthetic midlog-phase cells exhibited km's for uptake of about 55 micrometer mg2+ and 0.5 micrometer mn2+. the vmax's also differed be ... | 1978 | 641011 |
| an alternative pathway for the degradation of endogenous fructose during the catabolism of sucrose in rhodopseudomonas capsulata. | sucrose catabolism was studied in rhodopseudomonas capsulata. sucrose was hydrolysed by the action of a constitutive cytoplasmic sucrase. the use of a glucose-6-phosphate dehydrogenase-deficient mutant and radiorespirometric experiments demonstrated that both the glucose and fructose moieties of sucrose were catabolized via the entner-doudoroff pathway. this result was confirmed by enzyme analysis and studies on sugar assimilation. all the enzymes of the entner-doudoroff pathway were present in ... | 1978 | 641527 |
| the dye-linked alcohol dehydrogenase of rhodopseudomonas acidophila. comparison with dye-linked methanol dehydrogenases. | 1. a dye-linked alcohol dehydrogenase was purified 20-fold from extracts of rhodopseudomonas acidophila 10050 grown anaerobically in the light on methanol/hco3-. 2. the enzyme resembled many previously reported methanol dehydrogenases from other methylotrophic organisms in coupling to phenazine methosulphate, requiring ammonia as an activator, possessing a ph optimum of 9 and a mol.wt. of approx. 116000. in many other respects the enzyme showed singular properties. 3. the stability of the enzyme ... | 1978 | 646793 |
| the charging capacitance of the chromatophore membrane. | | 1978 | 658407 |
| hydrogen metabolism and nitrogen fixation in wild type and nif- mutants of rhodopseudomonas acidophila. | n2 fixation, c2h2 reduction and h2 production in rhodopseudomonas acidophila dsm 137 were shown to be stoichiometrically related in ratios of 1:2.8:2.8. the highest possible h2 oxidation rate has been calculated to be about 6 fold higher in rhodopseudomonas acidophila dsm 137 than the maximum rate of h2 production. nif- mutants were isolated and tested; all of them had lost their ability of reduce c2h2 and to produce h2. in two nif- mutants hydrogenase activity and the capacity for autotrophic g ... | 1978 | 667181 |
| regulatory properties of the nitrogenase from rhodopseudomonas palustris. | ammonium salts, glutamine, asparagine, and urea cause an immediate inactivation (switch-off) of light-dependent acetylene reduction in intact cells of the photosynthetic bacterium rhodopseudomonas palustris. this effect is reversible showing the same kinetic pattern of inactivation and reactivation with all effector compounds. its duration depends on the amount of effector added to the cells. both nitrogenase components are found catalytically active in a cell-free preparation after enzyme switc ... | 1978 | 678011 |
| growth of a photosynthetic bacterium anaerobically in darkness, supported by "oxidant-dependent" sugar fermentation. | rhodopseudomonas capsulata can obtain energy for growth from light (anaerobically) and can also grow heterotrophically in darkness using alternative energy conversion modes, namely, aerobic respiration or an unusual type of anaerobic catabolism of sugars. dark anaerobic growth with fructose as sole carbon and energy source is dependent on the presence of an "accessory" oxidant such as trimethylamine-n-oxide, is accompanied by production of lactate and other classical fermentation products, and y ... | 1978 | 678017 |
| [molecular organization of the long-wave complexes of purple photosynthesizing bacteria. effect of pronase on the b890 complex of chromatium minutissium and rhodopseudomonas palustris]. | the pronase action on the long-wave complexes b890 from two different purple bacteria has been investigated. differences in the kinetics of decrease of the reaction center photochemical activity of electron-donor activity of cytochromes and of destruction of bx890 (875) forms have been discovered. different rates of the proteolysis of rc proteins were revealed by sds-gel-electrophoresis. the heavy protein of rc was the first to degrade. the photochemical transformations deltaa890(875) in b890 co ... | 1978 | 683188 |
| effect of light nitrogenase function and synthesis in rhodopseudomonas capsulata. | the metabolic versatility of the purple nonsulfur photosynethetic bacterial permits the expression of either a phototrophic or a dark aerobic mode of growth. these organism also possess nitrogenase activity which may function under semiaerboic conditions. on the basis of these important properties, the light dependence of nitrogenase function and synthesis in rhodopseudomonas capsulata was investigated. nitrogenase activity was strictly dependent on light; no activity was observed in the dark, e ... | 1978 | 711666 |
| purification of (4fe-4s)1--2--ferredoxins (high-potential iron--sulfur proteins) from bacteria. | | 1978 | 713842 |
| crystallization and subunit composition of citrate lyase of rhodopseudomonas gelatinosa. | | 1978 | 729782 |
| light-induced oxygen reduction as a probe of electron transport between respiratory and photosynthetic components in membranes of rhodopseudomonas capsulata. | | 1978 | 742893 |
| alteration in concentration of carotenoids in rhodopseudomonas capsulata transferred from dark to light growth. | | 1978 | 744316 |
| orientation of reaction center and antenna chromophores in the photosynthetic membrane of rhodopseudomonas viridis. | | 1979 | 760778 |
| arrangement and interaction of pigment molecules in reaction centers of rhodopseudomonas viridis. photodichroism and circular dichroism of reaction centers at 100 k. | | 1979 | 760781 |
| recent studies on the chloroplast cytochrome b-559. | | 1975 | 766026 |
| isolation and analysis of the lipid a backbone. lipid a structure of lipopolysaccharides from various bacterial groups. | a degradation procedure of lipopolysaccharides was worked out which allows the isolation of the reduced backbone of lipid a in a total yield of between 20 and 30%. this procedure was applied to lipopolysaccharides of s forms (salmonella minnesota, shigella flexneri 5b, escherichia coli 086, e. coli 0111, xanthomonas sinensis, rhodopseudomonas gelatinosa) and r mutants (salmonella minnesota, shigella flexneri, 5b, e. coli bb9 and e. coli eh 100). chemical analysis, reaction with beta-n-acetyl-glu ... | 1976 | 770165 |
| localization of photosynthetic reaction centers by antibody binding to chromatophore membranes from rhodopseudomonas spheroides strain r26. | rabbit antiserum against highly purified reaction center preparations was shown to react specifically with a single component of chromatophore membranes from rhodopseudomonas spheroides strain r-26. the conjugate of purified gamma globulin and ferritin prepared with toluene diisocyanate was used to determine the localization of reaction centers in the chromatophore membranes. virtually no antibody was bound by intact membranes. after removing the 9nm atpase from these membranes by dilute edta ... | 1975 | 804932 |
| uptake of c4 dicarboxylates and pyruvate by rhodopseudomonas spheroides. | the uptake of c4 dicarboxylates by cells from exponential cultures of rhodopseudomonas spheroides followed saturation kinetics at concentrations below 100 mum with km values for succinate, malate, and fumarate of 2.7, 2.3, and 0.8, respectively. corresponding vmax values of 50, 52, and 67.5 nmol/min per mg of protein at 20 c were obtained. each of these compounds interfered competitively with uptake of the others, and a common transport system appears to be involved. fructose-grown cells took up ... | 1975 | 808529 |
| procaryote phylogeny iv: concerning the phylogenetic status of a photosynthetic bacterium. | the 16s ribosomal rna (30s subunit) of rhodopseudomonas spheroides has been characterized in terms of t1 ribonuclease digestion products. this "fingerprint" ultimately permits the placement of r. spheroides into a detailed procaryotic phylogenetic tree. given the number of major procaryotic lines that have been characterized in these terms to date, one can tentatively place the athiorhodaceae closer to the vibrio-enteric group than to the bacillaceae or cyanophyta. | 1975 | 809594 |
| carotenoid triplet states in reaction centers from rhodopseudomonas sphaeroides and rhodospirillum rubrum. | purified photochemical reaction centers from three strains of rhodopseudomonas sphaeroides and two of rhodospirillium rubrum were reduced with na2s2o4 so as to block their photochemical electron transfer reactions. they then were excited with flashes lasting 5-30 ns. in all cases, absorbance measurements showed that the flash caused the immediate formation of a transient state (pf) which had been detected previously in reaction centers from rps. sphaeroides strain r26. previous work has shown th ... | 1975 | 811259 |
| cross-reactivity of phenylalanyl-transfer ribonucleic acid ligases from different microorganisms. | the cross-reaction of phenylalanyl-transfer ribonucleic acid (trna) ligases from different microorganisms with antibodies raised against the purified enzyme from escherichia coli has been investigated. the results of immunotitration and immunodiffusion experiments and of the sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of immunoprecipitates revealed: (i) a high degree of immunochemical identity of this enzyme only within the family enterobacteriaceae; (ii) intermediate-to-w ... | 1975 | 811647 |
| [reversible photooxidation of bacteriochlorophylls a and b in aqueous solutions of detergents]. | | 1975 | 813982 |
| [control of atp metabolism in rhodopseudomonas spheroides]. | growth kinetics and cell composition (rna, protein, bacteriochlorophyll and adenosyl nucleotides phosphate) of rhodopseudomonas spheroides in photosynthetic growing culture with continuous illumination and various intermittent light regimens have been examined. the results indicated that bacteriochlorophyll and rna were controlled by atp level and protein synthesis and growth by the ability of the cells to utilize intracellular atp. | 1976 | 820451 |
| glycerol dissimilation and its regulation in bacteria. | | 1976 | 825019 |
| enzymatic modification for improving nutritional qualities and acceptability of proteins extracted from photosynthetic microorganisms spirulina maxima and rhodopseudomonas capsulatus. | the present study attempts to improve the proteins from a blue-green alga spirulina maxima and a non-sulfur purple bacterium rhodopseudomonas capsulatus through their peptic hydrolysis followed by plastein synthesis with papain. the former enzymatic process was effective in removing some photosynthetic pigments and flavors originating in the raw materials. the latter process was successful in incorporating limited amounts of methionine, lysine, and tryptophan, and thus to synthesize plasteins wh ... | 1976 | 828668 |
| synthesis and soluble pools of ribosomal proteins in rhodopseudomonas palustris. | the specific antibodies prepared against two purified ribosomal proteins (19 and 24) and total 66-s ribosomal proteins have been utilized to measure free ribosomal proteins in rhodopseudomonas palustris. the free ribosomal protein pool (66 s) amounts to 1-2% of the total soluble proteins of r. palustris. in addition, the size of free ribosomal protein pool (66 s) is calculated to be approx. 7% of the total ribosomal protein on the mature 66-s ribosomes from the pulse-labelling data. a study of t ... | 1977 | 831818 |
| the effect of nalidixic acid on the cell cycle of synchronous rhodopseudomonas palustris cultures. | the influence of the dna synthesis inhibitor, nalidixic acid, on the properties of synchronous cultures of selected rhodopseudomonas palustris swarmer cells was examined. there was little alteration in the changes in morphology, extinction, volume distribution and leucine incorporation up to bud development, and photosynthetic membrane lamellae were still synthesized de novo in the bud. however, there was no subsequent division, or flagellum or holdfast synthesis. instead cells elongated by cont ... | 1977 | 833569 |
| effects of incident light levels on photosynthetic membrane polypeptide composition and assembly in rhodopseudomonas sphaeroides. | cells of rhodopseudomonas sphaeroides were grown anaerobically with incident light levels ranging between 4,500 and 400 footcandles (ca. 48,420 and 4,304 lux). cells grown with the higher light levels had lower contents of total bacteriochlorophyll and incorporated l-[u-14c]leucine into membrane protein at higher rates than cells grown with lower light levels. the former cells also contained relatively lower amounts of light-harvesting membrane polypeptides as compared with the latter cells. in ... | 1977 | 838679 |
| h2 metabolism in the photosynthetic bacterium rhodopseudomonas capsulata: h2 production by growing cultures. | purple photosynthetic bacteria produce h2 from organic compounds by an anaerobic light-dependent electron transfer process in which nitrogenase functions as the terminal catalyst. it has been established that the h2-evolving function of nitrogenase is inhibited by n2 and ammonium salts, and is maximally expressed in cells growing photoheterotrophically with certain amino acids as sources of nitrogen. in the present studies with rhodopseudomonas capsulata, nutritional factors affecting the rate a ... | 1977 | 838685 |
| h2 metabolism in the photosynthetic bacterium rhodopseudomonas capsulata: production and utilization of h2 by resting cells. | photoproduction of h2 and activation of h2 for co2 reduction (photoreduction) by rhodopseudomonas capsulata are catalyzed by different enzyme systems. formation of h2 from organic compounds is mediated by nitrogenase and is nto inhibited by an atmosphere of 99% h2. cells grown photoheterotrophically on c4 dicarboxylic acids (with glutamate as n source) evolve h2 from the c4 acids and also from lactate and pyruvate; cells grown on c3 carbon sources, however, are inactive with the c4 acids, presum ... | 1977 | 838686 |
| [photoreduction of bacteriophenophytin b in the primary light reaction of rhodopseudomonas viridis chromatophores]. | photoconversions of the reaction center pigments in chromatophores of nonsulfur purple bacteria rhodopseudomonas viridis have been studied as a function of redox potential of medium (eh). it has been shown that at a decrease in the eh values from +400 mv to--100 divided by--600 mv a photo-induced accumulation of p980+ (oxidized primary electron donor in r. viridis) is replaced by the photoaccumulation of a reduced pigment complex p800 (bleaching of bacteriopheophytin b absorption bands at 545 an ... | 1977 | 861312 |
| ribosomal ribonucleic acid cistron homologies among hyphomicrobium and various other bacteria. | the extent of hybrid formation between the ribosomal ribonucleic acid (r-rna) of hyphomicrobium strain b-522 and deoxyribonucleic acid (dna) from bacteria of 21 different genera was examined. three generalized groupings were formed. group i (72-100%) consisted entirely of other strains of hyphomicrobium. representatives of the genera rhodopseudomonas, chromatium, caulobacter, prosthecomicrobium, rhodomicrobium, hyphomonas, and hyphomicrobium made up group ii (49-69%). the remaining gram-negative ... | 1977 | 861853 |
| isolation and spectral characteristics of the photochemical reaction center of rhodopseudomonas viridis. | a method is described for isolation of the rhodopseudomonas viridis reaction center complex free of altered, 685 nm absorbing pigment. this improved preparation contains two c-type cytochromes in the ratio p-960: cytochrome c-558: cytochrome c-553 of 1:2:2 to 3. the near infrared spectral forms of the reduced preparation are located at 790, 832, 846, and 987 nm at 77 k; the oxidized complex absorbs at 790, 808, 829 and approx. 1310 nm. the 790 nm band is attributed to bacteriophaeophytin b and t ... | 1977 | 870038 |
| [comparative study of the molecular organization of light-collecting complexes of chromatium minutissimum and rhodopseudomonas palustris]. | | 1977 | 872730 |
| the gene transfer agent of rhodopseudomonas capsulata. purification and characterization of its nucleic acid. | | 1977 | 879805 |