| a bioenergetic explanation for the selective vulnerability of renal medullary tubules to hypoxia. | 1. in order to explain the vulnerability of medullary thick ascending limb of henle's loop (mtal) during hypoxia, adenosine 5'-triphosphate (atp) content was measured in isolated rat mtal cells during control conditions and chemically induced hypoxia and compared with those in medullary collecting duct (mcd) cells. 2. basal atp levels in mtal and mcd were estimated as 3.6 and 2.1 mmol/l, respectively. antimycin a (5 mumol/l) decreased the atp content by 41% of the control value in the mtal cells ... | 1988 | 3416564 |
| citrate or ascorbate/citrate treatment of established corneal ulcers in the alkali-injured rabbit eye. | immediate treatment of alkali-injured eyes with citrate or ascorbate has previously been shown to prevent corneal ulceration and perforation in the rabbit. other experiments showed that while ascorbate treatment of established ulcers did not appear to lead to significant healing it did reduce perforations by prolonging the presence of descemetoceles. in the present experiment with established ulcers, alkali injuries were created with 1 n naoh in a 12 mm corneal well for 35 seconds. eyes were ent ... | 1988 | 3417403 |
| boholmycin, a new aminoglycoside antibiotic. i. production, isolation and properties. | a novel aminoglycoside antibiotic, boholmycin, was produced by streptomyces hygroscopicus h617-25 isolated from a soil sample collected in bohol island, the philippines. it has a pseudotetrasaccharide structure composed of a heptose, two aminosugars and dicarbamoyl-scyllo-inositol. intrinsic antibacterial activity of boholmycin is weak but it exhibits broad spectrum activity against gram-positive and gram-negative bacteria including aminoglycoside-resistant strains. boholmycin is non-toxic in mi ... | 1988 | 3417558 |
| evidence for arrested g2 cell subpopulation in rat liver inducible to mitosis. | the intraperitoneal administration of several substances (biliverdin, heat-killed bacteria and diatomaceous earth) to rats caused the prompt appearance of a mitotic wave in the liver. autoradiographic analysis of livers of treated animals showed no evidence of [3h]-thymidine uptake by mitotic hepatocytes. in addition, livers from xenobiotic-treated rats showed a very low thymidine kinase activity, close to that found in normal livers. this excludes the possibility that non-cycling cells move to ... | 1987 | 3502928 |
| malaria transmitted to humans by mosquitoes infected from cultured plasmodium falciparum. | malaria was transmitted to six normal human volunteers by mosquitoes infected from cultured gametocytes of plasmodium falciparum. this method, which offers advantages over other methods of infecting volunteers, will be useful for evaluating the efficacy of human malaria vaccines. | 1986 | 3511753 |
| cancer incidence and lifespan vs. alpha-particle dose in beagles. | young adult beagles were injected with graded activities of 239pu, 241am, 228th, 228ra or 226ra and observed throughout their lifespans. the vast majority of the dose was from alpha particles. the lifetime incidence of bone sarcoma increased with average skeletal dose, more or less linearly up to high incidence for 239pu, 241am, 228th and 226ra, but sigmoid fashion for 228ra. based on average skeletal dose, the toxicity of the emitters relative to 226ra = 1.0 was 239pu = 16.6 +/- 4.5, 241am = 5. ... | 1987 | 3570798 |
| campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true campylobacter sp. | comparison of partial 16s rrna sequences from representative campylobacter species indicates that the campylobacter species form a previously undescribed basic eubacterial group, which is related to the other major groups only by very deep branching. this analysis was extended to include the spiral bacterium associated with human gastritis, campylobacter pylori (formerly campylobacter pyloridis). the distance between c. pylori and the other campylobacter species is sufficient to exclude the pylo ... | 1987 | 3571163 |
| [effect of vinblastine on the proliferative and immune responses of the lymphoid cells of the draining lymph node and spleen in mice undergoing regional immunization with ram erythrocytes]. | it was found that vinblastine administered 48 hours after immunization of mice by sheep red cells produced a long-term (for up to 3 days) suppression of 3h-thymidine inclusion into lymphoid cells following a 8-12-hour latent period. this was accompanied with a complete elimination of humoral response of the draining lymph node and a five-fold decrease of the number of antibody-forming cells in the spleen. | 1987 | 3582634 |
| cardiac-specific troponin-i radioimmunoassay in the diagnosis of acute myocardial infarction. | the cardiac isotype of the myofibrillar contractile protein, troponin-i, is located specifically in the mammalian heart. a sensitive radioimmunoassay has been developed to detect human and nonhuman primate cardiac troponin-i in serum down to 10 ng/ml. immunochemical cross reactivity with skeletal troponin-i was only 2% and was species nonspecific. normal patient levels of cardiac troponin-i are about 10 ng/ml. in patients with acute myocardial infarction (n = 32), serum cardiac troponin-i was el ... | 1987 | 3591601 |
| cloning and expression of the genes of two fumarate reductase subunits from wolinella succinogenes. | the fumarate reductase complex of the anaerobic bacterium wolinella succinogenes catalyzes the electron transfer from menaquinol to fumarate. two structural genes coding for subunits of the enzyme have been cloned in escherichia coli. the genes were isolated from a lambda embl3 phage gene bank by immunological screening and subcloned in an expression vector. the genes frda and frdb, which encode the fad protein (frd a, mr 79,000) and the iron-sulfur protein (frd b, mr 31,000) of the fumarate red ... | 1987 | 3609021 |
| immunospecific red cell binding of iodine 125-labeled immunoglobulin g erythrocyte autoantibodies. | the primary interaction of autoantibodies with red cells has been studied by using labeled autoantibodies. immunoglobulin g red cell autoantibodies obtained from igg antiglobulin-positive normal blood donors were labeled with radioactive iodine and compared with alloanti-d with respect to their properties and binding behavior. iodine 125-labeled igg autoantibody migrated as a single homogeneous peak with the same relative mobility as human igg on sodium dodecyl sulfate-polyacrylamide gel electro ... | 1987 | 3611953 |
| x-ray refinement study on the binding of cytidylic acid (2'-cmp) to ribonuclease a. | the x-ray structure of the inhibitor complex of bovine ribonuclease a with cytidylic acid (2'-cmp) has been determined at 2.3 a (1 a = 0.1 nm) resolution and refined by restrained least-squares refinement to r = 0.132 for 5650 reflections. incorporation of the inhibitor molecule has occurred with little disturbance of the protein main-chain atoms, although significant displacement of some side-chain atoms has occurred, particularly in the region of the active site. the binding of 2'-cmp to ribon ... | 1987 | 3656443 |
| brain metabolic correlates of hypoxic-ischemic cerebral necrosis in mid-gestational sheep fetuses: significance of hypotension. | mid-gestational sheep fetuses exposed to marked hypoxia for 2 h remain brain intact if mabp is maintained above 30 mm hg. on the other hand, similarly hypoxic fetuses, if they experience reductions in mabp below 30 mm hg, develop foci of necrosis that predominantly affect hemispheric white matter and neostriatum. cortex damage is more restricted and is usually associated with more massive underlying white matter damage. the present study examines the brain metabolic basis for the important role ... | 1986 | 3733902 |
| induction of rheumatoid factors in mice by immune complexes of bacterial lipopolysaccharide with mouse igg antibody. | administration of 2,4,6-trinitrophenylated e. coli lipopolysaccharide (tnp-lps) complexed with mouse igg antibody to tnp specifically gave rise to a marked production of rheumatoid-like factors (rf) in the recipient mice, in contrast to the low and nonspecific rf production via polyclonal b cell activation by the same dosage of lps or tnp-lps alone. the rf activity induced by the lps immune complexes was associated with both igg and igm and directed primarily to the c gamma 2 region as judged by ... | 1986 | 3753937 |
| naloxone prevents hyperthermia induced convulsions in the immature rat. | there is increasing evidence suggesting that endogenous opioid peptides play a role both in temperature regulation and in the etiopathology of seizures. we have studied the effects of the opioid antagonist naloxone on hyperthermia-induced seizures in unrestrained 15 day old rat pups. saline injected control animals exposed to an ambient temperature of 40 degrees c for one hr showed a gradual increase in body temperature reaching a maximum of 42 degrees c at 50 min of exposure; at this time, all ... | 1986 | 3807466 |
| perisplenitis and perinephritis in the curtis-fitz-hugh syndrome. | four cases of the curtis-fitz-hugh syndrome diagnosed laparoscopically and with microbiological or serological evidence of chlamydial pelvic infection are reviewed. the case histories emphasize the part played by renal angle and left upper quadrant symptoms. in one patient the surface of the spleen was affected by the same classical inflammation normally seen on the surface of the liver. in 3 patients bilateral or left-sided renal angle pain and tenderness constituted the presenting features, or ... | 1987 | 3815024 |
| embryonic somatic nerve destruction with beta-bungarotoxin. | the effects and time course of a single injection of beta-bungarotoxin into e14 rat embryos were examined with an electron-microscopic study of development of the internal intercostal somatic nerve. within 24 h of injection, axons in this nerve became swollen and fused at points along their length. by 48 h after injection no component of the nerve remained in distal segments of ribcage; complete loss of axons and components of the nerve sheath from proximal regions took slightly longer. at later ... | 1987 | 3829119 |
| gonadotropin-releasing hormone within the organum vasculosum lamina terminalis in the ovariectomized, estrogen/progesterone-treated rat: a quantitative immunocytochemical study using image analysis. | no specific function has been ascribed to the high gonadotropin-releasing hormone (gnrh) content of the organum vasculosum lamina terminalis (ovlt). the objective of this study was to determine whether levels of gnrh within the ovlt are altered during cyclic gonadotropin secretion. gnrh levels were determined at various times during an estrogen/progesterone (e/p)-induced lh surge. groups of e/p and sesame oil-treated animals were decapitated at 12.00 h, 14.00 h, 16.00 h, 18.00 h, and 22.00 h fol ... | 1985 | 3896396 |
| [cloning of human leukocyte interferon cdna and a strategy for its production in e. coli]. | | 1985 | 3906813 |
| [cloning of human leukocyte interferon cdna and a strategy for its production in e. coli]. | | 1985 | 3906813 |
| the effects of anticalmodulin drugs on the ultrastructure of boar spermatozoa. | trifluoperazine, n-6-aminohexyl-5-chloro-1-naphthalene sulfonamide (w7), and calmidazolium are known to be calmodulin inhibitors and cell membrane soluble substances. in mammalian spermatozoa, calmodulin is present and is retained to mediate several sperm processes, such as sperm activation, sperm-egg fusion, microtubule disassembly, etc. we examined the effects of anticalmodulin drugs on the ultrastructure of freshly ejaculated boar spermatozoa. whereas all the drugs, at the low concentrations ... | 1985 | 3936711 |
| the effect of lysine acetylsalicylate on joint capsule mechanoreceptors in rats with polyarthritis. | joint capsule mechanoreceptors in arthritic rats are more sensitive to pressure than similar receptors in normal animals. this greater sensitivity was reversed by the intravenous or topical administration of lysine acetylsalicylate in anaesthetised rats in doses of 15 to 50 mgm asa-equivalent/kg. the reduction in sensitivity began within 5-10 min and reached a minimum mean value of 35% of the control after 35 to 40 min. during this period there was a negative linear or exponential relation betwe ... | 1985 | 3936727 |
| the effect of lysine acetylsalicylate on joint capsule mechanoreceptors in rats with polyarthritis. | joint capsule mechanoreceptors in arthritic rats are more sensitive to pressure than similar receptors in normal animals. this greater sensitivity was reversed by the intravenous or topical administration of lysine acetylsalicylate in anaesthetised rats in doses of 15 to 50 mgm asa-equivalent/kg. the reduction in sensitivity began within 5-10 min and reached a minimum mean value of 35% of the control after 35 to 40 min. during this period there was a negative linear or exponential relation betwe ... | 1985 | 3936727 |
| pseudoephedrine-induced hypertensive emergency: treatment with labetalol. | the case of a young man in hypertensive emergency following the ingestion of 840 mg of pseudoephedrine is reported. the hypertension, which peaked at 200/160 mm hg and was associated with severe headache and diaphoresis, responded expeditiously to two initial intravenous doses of labetalol. no rebound was observed following the administration of an additional dose in the intensive care unit, after which an uneventful one-day hospitalization ensued. the toxicity of pseudoephedrine is briefly revi ... | 1986 | 3947442 |
| the purification and some equilibrium properties of the nitrite reductase of the bacterium wolinella succinogenes. | the bacterium wolinella succinogenes produces a nitrite reductase enzyme that can be purified to homogeneity in high yield by a combination of detergent extraction, hydroxyapatite chromatography and mr fractionation. nitrite reductase activity is found to be present in both a high- and a low-mr fraction. the high-mr fraction has been shown to consist of the low-mr nitrite reductase enzyme associated with a hydrophobic 'binding protein'. the amino acid composition for both proteins is reported. t ... | 1986 | 3954752 |
| kinetic studies on the nitrite reductase of wolinella succinogenes. | the six haem groups of the nitrite reductase enzyme isolated from wolinella succinogenes are rapidly reduced by the addition of dithionite (s2o4(2-)). the reduction, however, is not homogeneous. two of the haem groups, namely those that show spectral characteristics typical of five-co-ordinated haem groups, are reduced in a dithionite-concentration-dependent fashion with a rate limit of 1.5 s-1. the other four haem groups, which show spectral characteristics very similar to those of normal six-c ... | 1986 | 3954753 |
| human calcitonin and malignant osteolysis. | | 1986 | 3960585 |
| [dose-dependent effect of amrinone on hemodynamics, myocardial circulation and myocardial energy requirement. an experimental study]. | this study was designed to assess the dose-dependent effects of amrinone (1, 2 and 4 mg/kg i.v.) on hemodynamics, myocardial blood flow and myocardial oxygen consumption in anesthetised closed chest dogs (n = 8). heart rate (hr), cardiac output, mean aortic pressure (map), left ventricular end-diastolic pressure (lvedp), maximum dp/dt (dp/dtmax), myocardial blood flow (mbf) and aorto-coronary sinus oxygen difference (avdo2 cor) were measured. cardiac index (ci), stroke volume index (svi), ejecti ... | 1985 | 3993153 |
| preliminary crystallographic study of an l-asparaginase from vibrio succinogenes. | crystals of an l-asparaginase from vibrio succinogenes were obtained with the hanging drop method from ammonium sulphate-containing solutions. the crystals belong to the orthorhombic space group p22(1)2(1) with unit cell dimensions of a = 71.3 a, b = 85.8 a, c = 114.0 a, and contain two tetrameric enzyme molecules per unit cell. there are two subunits in the asymmetric unit; a molecular dyad is coincident with the crystallographic dyad. the crystal lattice is similar to that reported for an esch ... | 1985 | 4032477 |
| concentrations of total and ionic calcium, inorganic phosphorus, magnesium, and protein in maternal and fetal blood and in amniotic fluid in parathyroidectomized pregnant rats. | | 1985 | 4032928 |
| the function of antibody and complement in the lysis of bacteria. | the factors controlling lysis of gram-negative bacteria by complement are being investigated systematically. the first question was how smooth salmonella minnesota, which has on its surface lipopolysaccharide with long o polysaccharide side chains, avoids lysis. rough organisms are serum sensitive. in both smooth and rough organisms, complement components are deposited on the surface and the lytic sequence proceeds to completion. however, with serum-resistant organisms the membrane attack comple ... | 1987 | 3317749 |
| effects of endotoxemia on pulmonary vascular resistances in unanesthetized sheep. | ten experiments were conducted on nine sheep to determine the effects of endotoxemia (1.0 microgram/kg iv over 15 min) on the vascular resistances of two segments of the pulmonary circulation. the first segment (s1) was from the main pulmonary artery to the site in the pulmonary veins corresponding to the pressure measured with a deflated and wedged 7-fr swan-ganz catheter. the second segment (s2) was from the wedge pressure measurement site to the left atrium. endotoxemia caused both pulmonary ... | 1987 | 3308813 |
| preliminary crystal structure of acinetobacter glutaminasificans glutaminase-asparaginase. | the preliminary structure of a glutaminase-asparaginase from acinetobacter glutaminasificans is reported. the structure was determined at 3.0-a resolution with a combination of phase information from multiple isomorphous replacement at 4-5-a resolution and phase improvement and extension by two density modification techniques. the electron density map was fitted by a polypeptide chain that was initially polyalanine. this was subsequently replaced by a polypeptide with an amino acid sequence in a ... | 1988 | 3275637 |
| electron paramagnetic resonance and magnetic circular dichroism studies of a hexa-heme nitrite reductase from wolinella succinogenes. | the nature of the heme centers in the hexa-heme dissimilatory nitrite reductase from the bacterium wolinella succinogenes has been investigated with epr and magnetic circular dichroism spectroscopy. the epr spectrum of the ferric enzyme is complex showing, in addition to magnetically isolated low-spin ferric hemes with g values of 2.93, 2.3 and 1.48, two sets of signals at g = 10.3, 3.7 and 4.8, 3.21, which we assign to two pairs of exchange coupled hemes. the mcd spectra show that the isolated ... | 1987 | 3036599 |
| respiration-linked proton flux in wolinella succinogenes during reduction of n-oxides. | formate uncoupled proton translocation in formate-grown wolinella succinogenes cells supplied with n-oxides as terminal electron acceptors. in suspensions containing kscn (but not valinomycin), h2 supported proton translocation when no3-, no2-, and no were provided as oxidants. h+/n-oxide ratios were 4.77 for no3-, 2.49 for no2-, and 1.75 for no. kscn inhibits n2o reduction thus precluding use of n2o as oxidant. repeated exposure of cells to no inhibited their ability to translocated protons wit ... | 1986 | 3004349 |
| polysaccharides as antiviral agents: antiviral activity of carrageenan. | a number of polysaccharides showed good antiviral activity against several animal viruses. at 5 micrograms/ml, carrageenan prevented the cell monolayer from destruction by herpes simplex virus type 1 (hsv-1) growth. at 10 micrograms/ml, carrageenan reduced the formation of new infectious hsv-1 by almost five logs. no cytotoxic effects were detected with concentrations of carrageenan up to 200 micrograms/ml. when 10 micrograms of carrageenan per ml was added at the beginning of hsv-1 infection of ... | 1987 | 2823697 |
| stability of midface augmentation: an experimental study of musculoskeletal interaction and fixation methods. | this study examined stability of vertical midface augmentation when different methods to alleviate potentially distracting muscles were used. eighteen adult monkeys underwent le fort i osteotomy with interpositional bone grafts. one group had wire fixation of the maxilla and bone graft. a second group underwent a similar procedure with the addition of myotomies of the masseter and temporalis muscles. a third group wore a bite-opening appliance before downgrafting. a fourth group underwent downgr ... | 1989 | 2795299 |
| double intussusception fatally complicated by clostridial infection in a dog (a case report). | a 10-month-old alsation dog with history of anorexia, diarrhoea, dehydration and vomition developed a double intussusception which affected the distal jejunum and proximal ileum. necropsy revealed the intussusception to be swollen and congested with fibrinous adhesions between the intussusceptum and intussuscepiens. two pieces of bone believed to be the inciting cause were found within the intussusceptum. clostridium welchii and clostridium bifermentans were isolated from the lesions. | 1989 | 2736386 |
| cbp2 protein promotes in vitro excision of a yeast mitochondrial group i intron. | the terminal intron (bi2) of the yeast mitochondrial cytochrome b gene is a group i intron capable of self-splicing in vitro at high concentrations of mg2+. excision of bi2 in vivo, however, requires a protein encoded by the nuclear gene cbp2. the cbp2 protein has been partially purified from wild-type yeast mitochondria and shown to promote splicing at physiological concentrations of mg2+. the self-splicing and protein-dependent splicing reactions utilized a guanosine nucleoside cofactor, the h ... | 1989 | 2685564 |
| amino acid sequences of the two major isoforms of troponin c from crayfish. | the primary structure of the two major isoforms (alpha and gamma) of troponin c (tnc) from crayfish tail muscle has been determined by the application of manual and automated edman degradation procedures to fragments generated by suitable chemical and proteolytic cleavages. both amino acid sequences commence with an acetylated methionyl residue and contain 150 amino acid residues, including a single proline residue at position 29 and 2 residues of tyrosine at positions 95 and 102. no cysteine or ... | 1989 | 2681191 |
| purification and some characteristics of a cytochrome c-containing nitrous oxide reductase from wolinella succinogenes. | nitrous oxide reductase from wolinella succinogenes was purified very nearly to homogeneity. the enzyme was found to be dimeric, with mr = 162,000 and subunit mr = 88,000, and to contain three copper atoms and one iron atom (as cytochrome c) per subunit. the oxidized enzyme exhibited absorption bands at 410 and 528 nm, and the dithionite-reduced enzyme, at 416, 520, and 550 nm. the isoelectric point was 8.6; specific activity was at 25 degrees c and ph 7.1, 160 mumol x min-1 x mg-1; and km was 7 ... | 1989 | 2536696 |
| the structure of the dihaem cytochrome b of fumarate reductase in wolinella succinogenes: circular dichroism and sequence analysis studies. | the fumarate reductase from wolinella succinogenes contains two haem groups with markedly different midpoint potentials (-20 mv and -200 mv). the enzyme is made up of three subunits, the lipophilic one of which (cytochrome b) ligates the haems. circular dichroism (cd) spectroscopy has been applied to the reductase in order to obtain information on the structure of the haems and of their environment. this approach is integrated with amino acid sequence comparison of the cytochrome b with other qu ... | 1991 | 2001380 |
| dissociated loss of vibration, joint position and discriminatory tactile senses in disease of spinal cord and brain. | the clinical functions of the posterior columns of the spinal cord and the signs of disease of these structures have been debated for years. todd in 1847 and schiff in 1858 knew the functions of the posterior columns and 10 years later brown-séquard knew as well. reynolds, romberg, and duchenne, each described a posterior column syndrome based on a disease in which the primary lesion was not in the posterior columns. in the last 150 years almost every white matter structure of the cord has been ... | 1991 | 1913366 |
| electron paramagnetic resonance observations on the cytochrome c-containing nitrous oxide reductase from wolinella succinogenes. | nitrous oxide reductase from wolinella succinogenes, an enzyme containing one heme c and four cu atoms/subunit of mr = 88,000, was studied by electron paramagnetic resonance (epr) at 9.2 ghz from 6 to 80 k. in the oxidized state, low spin ferric cytochrome c was observed with gz = 3.10 and an axial cu resonance was observed with g parallel = 2.17 and g perpendicular = 2.035. no signals were detected at g values greater than 3.10. for the cu resonance, six hyperfine lines each were observed in th ... | 1991 | 1846617 |
| cloning and nucleotide sequence of the structural genes encoding the formate dehydrogenase of wolinella succinogenes. | the formate dehydrogenase of wolinella succinogenes is a membraneous molybdo-enzyme which is involved in phosphorylative electron transport. the gene (fdha) encoding the largest subunit was isolated from a gene bank by immunological screening. the fdha gene was located in an apparent transcriptional unit (fdha,b,c,d) together with three more structural genes. the n-terminal sequences of three polypeptides present in the isolated enzyme were found to map within the fdha, b and c structural genes. ... | 1991 | 1781728 |
| reactivity of the bacillus subtilis succinate dehydrogenase complex with quinones. | the succinate dehydrogenase isolated from bacillus subtilis was found to catalyze the oxidation of succinate with hydrophilic quinones. either naphthoquinones or benzoquinones served as acceptors. the enzyme activity increased with the redox potential of the quinone. the highest turnover number was commensurate with that of the bacterial succinate respiration in vivo. the succinate dehydrogenase was similarly active in fumarate reduction with quinols. the highest activity was obtained with the m ... | 1991 | 1655027 |
| interaction of aminoalkylcarbamates of forskolin with adenylyl cyclase: synthesis of an iodinated derivative of forskolin with high affinity for adenylyl cyclase. | 7-(2-aminoethyl)aminocarbonyl-7-desacetylforskolin (7-aec-fsk) and 6-(2-aminoethyl)aminocarbonylforskolin (6-aec-fsk) were synthesized and tested for their ability to activate adenylyl cyclase and inhibit the high affinity binding of [3h]forskolin to bovine brain membranes. forskolin and 7-aec-fsk were equipotent in activating adenylyl cyclase, with ec50 values of about 4 microm, whereas 6-aec-fsk had an ec50 of about 2 microm. 6-aec-fsk and 7-aec-fsk stimulated adenylyl cyclase about 7-fold ove ... | 1992 | 1538712 |
| modulation of adhesion molecules on human large granular lymphocytes by interleukin-2 in vivo and in vitro. | the modulation of adhesion molecules on human large granular lymphocytes (lgl) by interleukin (il)-2 was investigated both in vivo and in vitro. intercellular adhesion molecule-1 (icam-1; cd54) expression increased on lgl of cancer patients receiving il-2 adoptive immunotherapy. icam-1 expression on lgl isolated by percoll gradient centrifugation, lgl purified, and expanded by adherence to plastic surfaces and lgl identified by leu 19 (cd56) monoclonal antibody were increased significantly in re ... | 1992 | 1347485 |
| construction and characterization of a single polypeptide chain containing two enzymatically active dihydrofolate reductase domains. | a single polypeptide chain containing two dihydrofolate reductase (dhfr) sequences from escherichia coli was constructed to determine if a repeat sequence fusion protein could be expressed in an active form. the possibility that intersequence interactions could play a significant role for this enzyme is suggested by the results of hall and frieden (1989, proc. natl acad. sci. usa, 86, 3060-3064) who observed a substantial decrease in the yield of active enzyme when folded in the presence of a la ... | 1992 | 1287660 |
| the formate dehydrogenase involved in electron transport from formate to fumarate in vibrio succinogenes. | 1. the formate dehydrogenase of vibrio succinogenes, which is involved in electron transport with fumarate as terminal acceptor, was solubilized with triton x-100 and purified some 200-fold by means of chromatography on hydroxyapatite, sucrose-density-gradient centrifugation and chromatography on deae-sephadex. gel filtration failed to increase the specific acitivity of the enzyme while gel electrophoresis in the presence of dodecylsulfate revealed that 73% of the protein of the preparation cons ... | 1979 | 428397 |
| modification of yeast phosphofructokinase by trypsin and subtilisin in the presence of effectors. | yeast phosphofructokinase was subjected to limited proteolysis by trypsin in the presence of different effectors. it could be demonstrated that the substrates mgatp and fructose-6-phosphate are able to protect the enzyme from inactivation by trypsin. other effectors like amp, adp, phosphoenolpyruvate, citrate and ammonium ions exhibit only negligible effects. during the first step of degradation consisting in the conversion of the subunits from mr 120,000 to 90,000 no significant effects of the ... | 1978 | 154818 |
| controlled-test evaluation of fenbendazole against natural infections of lungworms in calves. | | 1978 | 150090 |
| purification and characterization of l-asparaginase with anti-lymphoma activity from vibrio succinogenes. | homogeneols l-asparaginase with anti-lymphoma activity was prepared from vibrio succinogenes, an anaerobic bacterium from the bovine rumen. an overall yield of pure l-asparaginase of 40 to 45% and a specific activity of 200 +/- 2 iu per mg of protein was obtained. the pure enzyme can be stored at -20 degrees for at least 3 months with no loss of activity. the isoelectric point of the l-asparaginase is 8.74. no carbohydrate, phosphorus, tryptophan, disulfide, or sulfhydryl groups were detected. t ... | 1976 | 11211 |
| facilitatory effect of antidromic stimulation on milk ejection-related activation of oxytocin neurons during suckling in the rat. | oxytocin neurons of the paraventricular nucleus were identified by their antidromic response to stimulation of the neurohypophysis and a short high-frequency burst of spikes displayed before reflex milk ejection in the urethane-anesthetized lactating rat. during suckling, the milk ejection-related bursts recurred at regular intervals. stimulation of the neurohypophysis at 50-300 pulses/s for 0.5-6 s (current 1 ma) evoked additional burst(s) with the amplitude higher than those of spontaneously o ... | 1985 | 4047499 |
| cathodoluminescence applied to the study of human glomerulonephritis. | the cathodoluminescence (cl) emission of fluorescent species submitted to electron beam irradiation in a scanning electron microscope may be used to reveal the localisation of the emitter at microscopic level. this technique has been applied to the study of human renal biopsies with membraneous glomerulonephritis. cryosections of biopsies were stained with specific antibody, labelled either by fluorescein or by rhodamine. rhodamine exhibited a stronger luminescence and a greater resistance to th ... | 1985 | 4048267 |
| solitary osteochondroma occurring on the transverse process of the lumbar spine. a case report. | we have presented a case of an osteochondroma involving the transverse process of l3 in a 17-year-old man with no evidence of other osteochondromatous lesions. the growth of the lesion was extremely rapid but demonstrated benign histology. this patient was asymptomatic and had a normal physical examination despite the size of the lesion. the benign scoliosis in our patient appears to have been unrelated to and unaffected by the osteochondroma. whether the osteochondroma would have reached suffic ... | 1985 | 4049103 |
| cardiac antiarrhythmic evaluation of naloxone with or without propranolol using a modified chloroform-hypoxia screening test in the rat. | the effects of naloxone and propranolol on cardiac arrhythmias and durations from respiratory arrest to ventricular asystole and cardiac standstill were studied in unanaesthetized young rats induced to suffer respiratory arrest and exhibit ventricular fibrillation (vf) by a modified chloroform hypoxia technique. both naloxone and propranolol reduced the incidence of vf dose dependently, indicating that they have antiarrhythmic effects. addition of naloxone to propranolol shifted the dose-respons ... | 1985 | 4092371 |
| ampicillin compared with penicillin and chloramphenicol combined in the treatment of bacterial meningitis. | | 1972 | 4149029 |
| distribution of tyrosine alpha-ketoglutarate transaminase activity in kupffer cell and whole liver fractions of rat liver. | | 1973 | 4149538 |
| [leptotrichia buccalis--culture, biochemistry and antibiotics]. | | 1974 | 4154653 |
| regulation of rat liver beta-hydroxy-beta-methylglutaryl-coa reductase activity by cholesterol. | | 1973 | 4202331 |
| the chemical composition and ultrastructure of the cell wall of scenedesmus obliquus. ii. amino acids, proteins and antigens. | | 1973 | 4206341 |
| transformation of bacillus subtilis in alpha-amylase productivity by deoxyribonucleic acid from b. subtilis var. amylosacchariticus. | deoxyribonucleic acid (dna) of bacillus subtilis var. amylosacchariticus showed almost the same ability as b. subtilis marburg to induce transfer of several genetic markers in dna-mediated transformation. dna-dna hybridization data also showed an intimate relationship between the two strains. genetic elements involved in the production of extracellular alpha-amylase (ec 3.2.1.1.) in b. subtilis var. amylosacchariticus were studied by using dna-mediated transformation. two marburg derivatives, na ... | 1974 | 4215793 |
| coupling of glycosaminoglycans to agarose beads (sepharose 4b). | 1. heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. the bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2. the uptake of glycosaminoglycan during the coupling procedure is about 0.9mg/ml of wet gel. however, direct analysis of washed and freeze-dried gels reveals that only about one-third of this amount is firmly attached to ... | 1971 | 4257093 |
| studies on the acetylcholine-stimulated incorporation of radioactive inorganic orthophosphate into the phospholipid of brain particulate preparations. ii. subcellular distribution of enzymic activity. | | 1966 | 4288042 |
| [experiences with virostatic therapy of herpetic keratitis]. | | 1971 | 4324844 |
| ontogeny of adrenal function in fetal and neonatal rhesus monkeys: in vitro corticosteroidogenesis. | | 1971 | 4325212 |
| influence of prostaglandins on autonomic responses. | | 1971 | 4330893 |
| glucose fermentation products in ruminococcus albus grown in continuous culture with vibrio succinogenes: changes caused by interspecies transfer of h 2 . | the influence of a h(2)-utilizing organism, vibrio succinogenes, on the fermentation of limiting amounts of glucose by a carbohydrate-fermenting, h(2)-producing organism, ruminococcus albus, was studied in continuous cultures. growth of v. succinogenes depended on the production of h(2) from glucose by r. albus. v. succinogenes used the h(2) produced by r. albus to obtain energy for growth by reducing fumarate in the medium. fumarate was not metabolized by r. albus alone. the only products detec ... | 1973 | 4351387 |
| relationship between antihistamine and antidepressant activity in hexahydroindenopyridines. | | 1972 | 4402485 |
| [development of the adrenal glands of the mouse. i. prenatal development (author's transl)]. | | 1974 | 4409165 |
| [development of the adrenal glands of the mouse. i. prenatal development (author's transl)]. | | 1974 | 4409165 |
| [proceedings: perinatal tumor induction using ethyl nitroso-urea: a contribution on the question of organotropism of alkylating resorptive careinogens in various mouse strains]. | | 1974 | 4460558 |
| proceedings: the electron transport-coupled phosphorylation of the anaerobe vibrio succinogenes. | | 1974 | 4461538 |
| ftc-insulin conjugates. i. preparation of fluorescein thiocarbamyl-insulin conjugates. | | 1973 | 4579445 |
| [xenogeneic kidney transplantation in closely related species (fox-dog) (author's transl)]. | | 1973 | 4584964 |
| [toxic properties of the blood serum of cattle leukemia]. | | 1973 | 4585668 |
| the effect of lactation on the absorption of 203 pb and 47 ca in rats. | | 1972 | 4674564 |
| preliminary data on the possible hypnogenic role of adenosine. | | 1973 | 4723335 |
| repressor and rex product of coliphage lambda: lack of collaboration and joint controls. | | 1973 | 4725965 |
| [determination of antibodies in hyperergic measles encephalitis]. | | 1973 | 4731035 |
| a simplified digital method for predicting anesthetic uptake and distribution. | | 1973 | 4777734 |
| sensitization and centre-surround antagonism in necturus retina. | 1. the impulse discharge of ganglion cells and the proximal negative response were recorded with extracellular micro-electrodes in necturus retina and spatial influences of background illumination studied.2. when a steady background of constant illuminance was extended in diameter from 0.25 to 1 mm, the response to a small concentrically placed test flash was enhanced (sensitization).3. this sensitization was tonic, showed marked spatial summation, was also found by surrounding a central backgro ... | 1974 | 4822576 |
| l-asparaginase production by the rumen anaerobe vibrio succinogenes. | the rumen anaerobe vibrio succinogenes possesses a constitutive l-asparaginase. the amount of enzyme produced is affected by the compound supplied to the organism to generate the fumaric acid it requires as a terminal electron acceptor. when nitrate is provided as the terminal electron acceptor, the amount of enzyme produced is affected by the compound provided to satisfy the nutritional requirement of the organism for succinic acid. specific activities of up to 8.4 iu/mg of protein in cell-free ... | 1974 | 4855647 |
| [hemoglobin of the african newborn infant. results of an investigation in senegal]. | | 1968 | 4894442 |
| pituitary and hypothalamic endocrine changes associated with reproductive development of holstein bulls. | | 1968 | 4908666 |
| steroid diabetes in the guinea pig. studies on islet-cell ultrastructure and regeneration. | | 1970 | 4908764 |
| characterization of the late steps of microbial heme synthesis: conversion of coproporphyrinogen to protoporphyrin. | cell-free extracts of various cytochrome-containing, heterotrophic microorganisms were examined for ability to convert coproporphyrinogen to protoporphyrin. extracts of escherichia coli and pseudomonas denitrificans readily accumulated large amounts of protoporphyrin when assayed under aerobic conditions. however, protoporphyrin did not accumulate under either aerobic or anaerobic conditions of assay or in the presence of various supplements in extracts of the aerobe micrococcus lysodeikticus, t ... | 1971 | 4935319 |
| lysis of vibrio succinogenes by ethylenediamine-tetraacetic acid or lysozyme. | wolin, m. j. (university of illinois, urbana). lysis of vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. j. bacteriol. 91:1781-1786. 1966.-cell suspensions of vibrio succinogenes are lysed by ethylenediaminetetraacetic acid (edta) or lysozyme. lysis occurs at alkaline ph and is prevented by 0.15 m nacl or kcl or 0.3 m sucrose. the addition of 10(-3)m mg(++), 10(-3)m spermine, or 10(-2)m ca(++) prevents lysozyme lysis, and 10(-4)m spermine prevents edta lysis. edta lysis leads ... | 1966 | 4957020 |
| solubilization and reconstitution of a particulate hydrogenase from vibrio succinogenes. | | 1966 | 4958911 |
| [the modifications of the thymus in the course of experimental tuberculosis in the mouse]. | | 1968 | 4968741 |
| chemical constituents and hydrogenase binding in cell envelopes of vibrio succinogenes. | the particulate hydrogenase of vibrio succinogenes is solubilized during treatment of cell envelopes at ph 11.0. alkali-solubilized enzyme requires sulfhydryl compounds for activity. at neutral ph, soluble enzyme is reincorporated into alkalitreated cell envelopes and no longer requires an additional activator. in the present study, cell envelopes prepared by lysing cells with ethylenediaminetetraacetic acid plus lysozyme (edta-lysozyme) were used to determine the chemical composition of cell en ... | 1969 | 4976463 |
| relationship between phosphates and alkaline phosphatase of anabaena flos-aquae in continuous culture. | | 1971 | 5001948 |
| requirement of succinate for the growth of vibrio succinogenes. | vibrio succinogenes required relatively small amounts of succinate for growth when formate plus nitrate was supplied as the energy source. the requirement for succinate was not apparent when formate plus fumarate was the energy source because fumarate is reduced to succinate. l-asparagine, fumarate, and malate replaced succinate, and it appears likely that they do so by being converted to succinate. formate plus l-aspartate or l-asparagine served as energy sources for growth. the stoichiometry o ... | 1972 | 5058443 |
| [gamma glutamyl carboxypeptidase from chicken pancreas]. | | 1972 | 5073874 |
| hydrogenase measurement with photochemically reduced methyl viologen. | methyl viologen was reduced photochemically in the presence of proflavine and ethylenediaminetetraacetic acid. the reduced methyl viologen was oxidized by hydrogenase from vibrio succinogenes. h(2) and oxidized methyl viologen were the products. hydrogenase activity was determined by spectrophotometric measurement of the disappearance of reduced methyl viologen at 600 nm. the extinction coefficient of reduced methyl viologen was determined and is 8.25 mm(-1) x cm(-1) at 600 nm. optimal condition ... | 1969 | 5781586 |
| taxonomy of anaerobes: present state of the art. | results of nucleic acid studies, which indicate relationships among strains and species more clearly than do usual phenotypic tests, have led to new bacteriologic nomenclature. some major changes in bacteroides include the recognition of three species (bacteroides melaninogenicus, bacteroides denticola, and bacteroides loescheii) formerly grouped in b. melaninogenicus subspecies melaninogenicus; two species (bacteroides intermedius with two closely related homology groups and bacteroides corpori ... | 1984 | 6201990 |
| an essential sulfhydryl group at the substrate site of the fumarate reductase of vibrio succinogenes. | | 1980 | 6250897 |
| iron-sulphur clusters in fumarate reductase from vibrio succinogenes. | (1) the fumarate reductase complex from vibrio succinogenes contains one fad molecule, one [4fe-4s]3+(3+,2+) and one [2fe-2s]2+(2+,1+) cluster per enzyme molecule. both clusters can be partly reduced by succinate. in the presence of excess na2s2o4 and fumarate, the [2fe-2s] cluster is completely oxidized, whereas the other cluster is largely reduced. (2) the [2fe-2s] cluster is localized in the mr, 31,000 subunit. the epr spectrum of the reduced cluster in the isolated subunit differs slightly i ... | 1981 | 6271222 |
| can gardnerella vaginalis and anaerobic curved rods attach to vaginal epithelial cells in vitro, resulting in clue cells? | vaginal epithelial cells were taken from asymptomatic patients and from patients with non-specific vaginosis. four bacterial species-gardnerella vaginalis, wolinella succinogenes and short and long anaerobic curved rods-were tested in vitro regarding attachment to the epithelial cells. in most experiments the curved rods and w. succinogenes attached to the cells. less often, incubation with g. vaginalis resulted in cell attachment. there was no difference in attachment ability between the short ... | 1984 | 6336133 |