| [two cases of infective endocarditis caused by haemophilus aphrophilus and actinobacillus actinomycetemcomitans: case reports and microbiological studies]. | | 1984 | 6086781 |
| classification and identification of actinobacillus actinomycetemcomitans and haemophilus aphrophilus by cluster analysis and deoxyribonucleic acid hybridizations. | | 1982 | 6219206 |
| relative proportions of haemophilus species in the throat of healthy children and adults. | to determine normal proportions of pharyngeal haemophilus species, qualitative and quantitative mapping of the species in the pharynx of ten healthy children and ten healthy adults was carried out using a selective and a non-selective medium. haemophilus organisms were present in all samples, comprising approximately 10% of the total cultivable flora (range 0.6-36.9%). haemophilus parainfluenzae was a member of the normal flora throughout life, constituting 74% of pharyngeal haemophilus organism ... | 1984 | 6332018 |
| identification and characterization of the major cell envelope proteins of oral strains of actinobacillus actinomycetemcomitans. | the major cell envelope protein compositions of seven actinobacillus actinomycetemcomitans strains of human origin were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the major envelope polypeptides were homogeneous, in relation to molecular weight, in all of the strains that were examined. the characterization of the five major proteins, designated env1 through env5, in the leukotoxic strain y4 revealed that proteins env2 to -5 may reside in the outer membrane as suggest ... | 1983 | 6401694 |
| serology of oral actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease. | actinobacillus actinomycetemcomitans from the human oral cavity was serologically characterized with rabbit antisera to the type strain nctc 9710; a number of reference strains, including y4, atcc 29522, atcc 29523, atcc 29524, nctc 9709; and our own isolates representative of each of 10 biotypes. using immunoabsorbed antisera, we identified three distinct serotypes by immunodiffusion and indirect immunofluorescence. serotype a was represented by atcc 29523 and sunyab 75; serotype b was represen ... | 1983 | 6407997 |
| vertebral osteomyelitis due to coccobacilli of the hb group. | three cases of pyogenic vertebral osteomyelitis occurred in which unusual, fastidious, gram negative coccobacilli belonging to the "hb" group were isolated. the organisms were haemophilus aphrophilus in case 1, intermediate between h aphrophilus and actinobacillus actinomycetemcomitans in case 2, and eikenella corrodens in case 3. all hb bacteria are sensitive to a wide range of antibiotics. | 1983 | 6416539 |
| rapid identification of capnocytophaga isolated from septicemic patients. | four capnocytophaga strains from blood cultures of immunocompromised patients with malignant disease and the type strains of three capnocytophaga species were examined and compared to strains representing five other genera that are hard to differentiate from capnocytophaga. with three rapid identification methods, negative catalase and oxidase reactions and positive onpg assay, capnocytophaga was easily separated from eikenella corrodens, actinobacillus actinomycetemcomitans, cardiobacterium hom ... | 1984 | 6468367 |
| increased frequency of isolation of pasteurella and actinobacillus species and related organisms. | ninety-six clinical isolates of pasteurella, actinobacillus and related organisms were submitted to our reference laboratory for identification. the procedures for detecting the 11 identified species, pasteurella multocida, pasteurella haemolytica, pasteurella ureae, pasteurella pneumotropica, actinobacillus lignieresii, actinobacillus equuli, actinobacillus actinomycetemcomitans, haemophilus aphrophilus, cardiobacterium hominis, and two unnamed species of cdc groups, hb-5 and ef-4, are describe ... | 1984 | 6468368 |
| distribution of oral haemophilus species in dental plaque from a large adult population. | the periodontal status of maxillary first molars in 284 young adults demonstrating near-health to early disease was evaluated, and supragingival and subgingival plaque samples were collected. plaque samples were processed anaerobically, enumerated microscopically for bacterial morphotypes, and cultivated on various media to enumerate the microflora. although haemophili were ubiquitous (recovered in 98.5 and 96.2% of the supragingival and subgingival plaque samples, respectively), 50% of the resp ... | 1984 | 6500711 |
| differentiation between actinobacillus actinomycetemcomitans and haemophilus aphrophilus based on carbohydrates in lipopolysaccharide. | in the present study, the closely related facultative, gram-negative rods, actinobacillus actinomycetemcomitans and haemophilus aphrophilus, were distinguished taxonomically by means of their carbohydrate composition in phenol-extracted lipopolysaccharide. both a. actinomycetemcomitans and h. aphrophilus lipopolysaccharide contained rhamnose, fucose, galactose, glucose, l-glycero-d-mannoheptose, galactosamine, and glucosamine. the content of galactose was approximately twice as high in lipopolys ... | 1984 | 6511846 |
| haemophilus aphrophilus endocarditis. | | 1984 | 6512618 |
| whole-cell methanolysis as a rapid method for differentiation between actinobacillus actinomycetemcomitans and haemophilus aphrophilus. | | 1984 | 6520182 |
| special topics in antimicrobial susceptibility testing: test accuracy against methicillin-resistant staphylococcus aureus, pneumococci, and the sensitivity of beta-lactamase methods. | three contemporary problems in antimicrobial susceptibility testing were assessed by the cap-microbiology surveys program in 1982. a penicillin-resistant streptococcus pneumoniae was categorized correctly as resistant by nearly 78% of infectious disease survey subscribers. this rate compares with a 15% accuracy in the 1981 surveys challenge, and all results reported from the nccls recommended 1 microgram oxacillin screening test correctly found penicillin resistance. further improvement in the m ... | 1983 | 6605086 |
| cardiobacterium hominis: review of microbiologic and clinical features. | cardiobacterium hominis, like other fastidious, opportunistic gram-negative bacilli, including actinobacillus actinomycetemcomitans, haemophilus aphrophilus, and eikenella corrodens, is increasingly recognized as a cause of human disease. in this review the microbiologic and clinical features of c. hominis are discussed. the findings are based on observations of two infected patients (the case history of one was reported previously) and on reports in the literature of 32 others. microbiologicall ... | 1983 | 6622885 |
| determination of acids in whole lipopolysaccharide and in free lipid a from actinobacillus actinomycetemcomitans and haemophilus aphrophilus. | acids from whole lipopolysaccharide and free lipid a of the closely related bacteria actinobacillus actinomycetemcomitans and haemophilus aphrophilus were determined by gas chromatography and gas chromatography-mass spectrometry. in whole lipopolysaccharide, 3-hydroxymyristic acid was most abundant, followed by myristic and 3-deoxy-d-manno-2-octulosonic acid. in the lipid a moiety, myristic acid dominated, followed by 3-hydroxymyristic acid. the acid composition of whole lipopolysaccharide and f ... | 1984 | 6746814 |
| determination of bound cellular fatty acids in actinobacillus actinomycetemcomitans and haemophilus aphrophilus by gas chromatography and gas chromatography-mass spectrometry. | | 1984 | 6746820 |
| abscess formation by haemophilus paraphrophilus secondary to a submandibular salivary calculus. | | 1983 | 6886439 |
| [haemophilus endocarditis. a case of haemophilus aphrophilus endocarditis in a patient with aortic and mitral valve prosthesis]. | | 1982 | 6923792 |
| enzymatic characterization of some oral and nonoral gram-negative bacteria with the api zym system. | the api zym system (analytab products, plainview, n.y.), containing 19 chromogenic substrates, was utilized semiquantitatively to detect extracellular acid and alkaline phosphatases, aminopeptidases, proteases, esterase-lipase, phosphoamidase, and glycosidases in 128 oral and nonoral isolates of black-pigmented bacteroides, actinobacillus, haemophilus aphrophilus, capnocytophaga, fusobacterium nucleatum, wolinella recta, and veillonella parvula. in the black-pigmented bacteroides group of organi ... | 1981 | 7026598 |
| a selective medium for actinobacillus actinomycetemcomitans and the incidence of the organism in juvenile periodontitis. | a selective medium, malachite green bacitracin agar, was developed for the isolation of actinobacillus actinomycetemcomitans from subgingival plaque of periodontally diseased patients. the medium consisted of trypticase soy agar 40 gm/liter, bacitracin 128 micrograms/ml, malachite green 8 micrograms/ml and 5% defibrinated sheep blood. the medium, when incubated in an atmosphere of air plus 10% co2 for 5 days, permitted greater than 80% recovery of pure cultures of a. actinomycetemcomitans when c ... | 1981 | 7028938 |
| salient biochemical characters of actinobacillus actinomycetemcomitans. | a total of 136 strains of actinobacillus actinomycetemcomitans were studied for 135 features. all isolates were small nonmotile capnophilic gram-negative rods which grew with no requirement of x or v growth factors. they all decomposed hydrogen peroxide, were oxidase-negative and benzidine-positive, reduced nitrate, produced strong alkaline and acid phosphatases, and fermented fructose, glucose and mannose. variable fermentation results were obtained with dextrin, maltose, mannitol and xylose. s ... | 1982 | 7065813 |
| selective medium for isolation of actinobacillus actinomycetemcomitans. | a selective medium, tsbv (tryptic soy-serum-bacitracin-vancomycin) agar, was developed for the isolation of actinobacillus actinomycetemcomitans, tsbv agar contained (per liter) 40 g of tryptic soy agar, 1 g of yeast extract, 100 ml of horse serum. 75 mg of bacitracin, and 5 mg of vancomycin. the tsbv medium suppressed most oral species and permitted significantly higher recovery of a. actinomycetemcomitans than nonselective blood agar medium. the distinct colonial morphology and positive catala ... | 1982 | 7068837 |
| [haemophilus aphrophilus endocarditis]. | | 1982 | 7117973 |
| [diagnosis of vertebral diseases by vertebral biopsy]. | vertebral biopsies were performed in 28 patients, 17 males and 11 females, because of vertebral body and disc changes between june 1978 and may 1982. in two patients biopsy was performed in two segments. punctures were done in local and superficial anaesthesia of the vertebral body periosteum during continuous fluoroscopy control using jamshidi's instrumentation. aspiration and tissue cylinders were evaluated histologically and bacteriologically. there were no complications among the 8 thoracic ... | 1982 | 7140565 |
| bacteremia and meningitis associated with haemophilus aphrophilus infection in a previously healthy child. | | 1982 | 7152687 |
| soft tissue infection associated with haemophilus aphrophilus. | | 1981 | 7243479 |
| cellular fatty acid and soluble protein composition of actinobacillus actinomycetemcomitans and related organisms. | the cellular fatty acid and protein content of twenty-five representative strains of actinobacillus actinomycetecomitans isolated from juvenile and adult periodontitis patients was compared to that of 15 reference strains of oral and nonoral actinobacillus species and haemophilus aphrophilus. trimethylsilyl derivatives of the fatty acid methyl esters were analyzed by gas-liquid chromatography. the predominant fatty acids of all 40 strains examined were 14:0, 3-oh 14:0, 16 delta, and 16:0. actino ... | 1981 | 7287893 |
| cellular fatty acid composition of actinobacillus actinomycetemcomitans and haemophilus aphrophilus. | strains of actinobacillus actinomycetemcomitans isolated from deep pockets of patients with juvenile periodontitis were analyzed for their content of cellular fatty acids. oral haemophilus strains, morphologically and biochemically similar to haemophilus aphrophilus, were also examined for their content of cellular fatty acids. the extractable lipids of the actinobacilli represented approximately 10% of the cell dry weight, with the bound lipids representing 2 to 5%. the major fatty acids consis ... | 1980 | 7430333 |
| morphology and ultrastructure of oral strains of actinobacillus actinomycetemcomitans and haemophilus aphrophilus. | selected human oral and nonoral strains of the genera actinobacillus and haemophilus were examined by transmission and scanning electron microscopy. the strains examined were morphologically identical to recognized actinobacillus actinomycetemcomitans, haemophilus aphrophilus, and haemophilus paraphrophilus. by transmission electron microscopy, the cells were typically gram negative in morphology, with several strains possessing some extracellular ruthenium red-staining polymeric material. numer ... | 1980 | 7439996 |
| use of a nonradioactive genetic probe identified, synthesized, and labeled in the polymerase chain reaction. | this study introduces a strategy to identify and produce sequences useful as genetic markers, or native genetic probes for dna-dna hybridization in bacterial strains where the genetics is not well described. actinobacillus actinomy-cetemcomitans (a.a.) was used as an example. fifty ng genomic dna from a.a. atcc 33384 and haemophilus aphrophilus atcc 33389 was amplified in a thermocycler using a single 10-mer primer. the pcr products were separated by electrophoresis on a 1% submarine agarose gel ... | 1994 | 7521967 |
| [haemophilus aphrophilus isolated from the blood of a patient with infective endocarditis]. | on july 1994, a 62-year-old female, having a history of mitral regurgitation, was admitted because of high fever, hematuria and conjunctival petechiae. she was diagnosed as having infective endocarditis with mitral valve vegetation proved by ultrasonic cardiography. the gram negative rods were isolated from blood cultures performed five times, performed prior to the administration of antibiotics. the isolates were identified as strains of h. aphrophilus. after two days of treatment with pcg (12 ... | 1995 | 7561248 |
| molecular and immunological characterization of a 64-kda protein of actinobacillus actinomycetemcomitans. | the 64-kda protein to which about half the sera from patients with localized juvenile periodontitis and rapidly progressive periodontitis reacted strongly was purified from actinobacillus actinomycetemcomitans y4. determination of the n-terminal sequence of the protein revealed that it was a groel-like protein. the dna fragment containing the groel gene of a. actinomycetemcomitans was amplified by polymerase chain reaction, and the groesl operon was cloned by using colony hybridization with the ... | 1995 | 7567064 |
| outpatient treatment of endocarditis in a clinic-based program in argentina. | the major cost in the treatment of infective endocarditis (ie) is the length of hospitalization required for the administration of intravenous antibiotics. this can be reduced by substituting shorter regimens and by the introduction of outpatient parenteral antibiotic therapy (opat). careful selection of patients is vital for the success of opat in ie. the patients should be hemodynamically stable and without clinical complications. the delivery of opat for ie followed a clinical and home-based ... | 1995 | 7588859 |
| haemophilus paraphrophilus endocarditis: case report and review. | endocarditis due to haemophilus paraphrophilus is an uncommon disease. we report a case of h. paraphrophilus endocarditis with embolic complications in which the causative organism was resistant to beta-lactam antibiotics. before april 1994, 16 cases of h. paraphrophilus endocarditis had been reported. infection by this organism usually affects a previously damaged mitral valve. we emphasize the fastidiousness of the organism and the high incidence of embolic complications, which determine the o ... | 1995 | 7620027 |
| genetic relationship of strains of haemophilus aphrophilus, h. paraphrophilus, and actinobacillus actinomycetemcomitans studied by ribotyping. | strains of h. aphrophilus, h. paraphrophilus, and a. actinomycetemcomitans are phenotypically very similar. ribotyping of 24 strains of h. aphrophilus, 22 strains of h. paraphrophilus, 8 strains of a. actinomycetemcomitans, and one strain each of the species pasteurella aerogenes, h. parahaemolyticus, and the genus capnocytophaga was studied using a non-radioactive digoxigenin labelled probe based on e. coli 16s- and 23s-ribosomal rna. restriction fragments were generated using restriction enzym ... | 1993 | 7690272 |
| haemophilus paraphrophilus infection: a pitfall in laboratory diagnosis. | many clinical laboratories have difficulty in identifying a group of organisms which are catalase negative, oxidase positive, gram negative rods. we describe a case of purulent sacroiliitis due to haemophilus paraphrophilus where the organism was initially misidentified as eikenella corrodens leading to inappropriate antimicrobial chemotherapy. we review the strains of h. paraphrophilus and e. corrodens that were identified by the national collection of type cultures over the last ten years. onl ... | 1995 | 7751671 |
| haemophilus paraphrophilus--an unusual cause of endocarditis. | | 1994 | 7848169 |
| single polymerase chain reaction for the detection of tetracycline-resistant determinants tet k and tet l. | oligonucleotide primers were used in polymerase chain reaction assays to detect tetracycline-resistant determinants tet k and tet l. forty-three isolates representing 11 genera carrying tet k and/or tet l determinants gave appropriate pcr products. the pcr products hybridized with labelled tet k or tet l probes, and differentiated the tet k from the tet l pcr products. we confirmed that two haemophilus aphrophilus carried the tet k determinant and two veillonella parvula carried the tet l determ ... | 1994 | 7877638 |
| [haemophilus aphrophilus bacteremia and diagnostic criteria of infectious endocarditis]. | | 1994 | 7981295 |
| haemophilus aphrophilus as a rare cause of arthritis. | a case of septic arthritis due to haemophilus aphrophilus is presented. this organism has rarely been reported as a cause of bone or joint infections. we believe this is the third reported case of septic arthritis caused by this microorganism. we review the clinical and bacteriologic findings and the previously reported cases of infection caused by h. aphrophilus. treatment with ceftriaxone was followed by full recovery without sequelae. | 1994 | 7986906 |
| binding of human hemoglobin by haemophilus influenzae. | binding of biotinylated human hemoglobin to haemophilus influenzae was detected when organisms were grown in heme-deplete, but not heme-replete, conditions. hemoglobin binding was completely inhibited by a 100-fold excess of unlabelled human hemoglobin or human hemoglobin complexed with human haptoglobin. binding was only partially inhibited by rat hemoglobin, bovine hemoglobin, human globin, and bovine globin, and not at all by heme, human serum albumin, bovine serum albumin, human transferrin, ... | 1994 | 8020748 |
| liver abscess following blunt trauma: a case report and review of the literature. | we report a case of haemophilus paraphrophilus causing primary liver abscesses after blunt nonpenetrating trauma. a 32-year-old previously healthy white man sustained a back injury 2 months prior to admission with fever, chills, and night sweats. a computed tomography (ct) scan-directed needle aspirate of several hypoechoic hepatic lesions grew h paraphrophilus. recent blunt trauma to the lower back may have contributed to the localization of this infection to an area of contusion or hematoma wi ... | 1994 | 8052889 |
| case report of spinal epidural abscess caused by haemophilus paraphrophilus. | haemophilus paraphrophilus was recovered in pure culture from purulent material collected at surgery from a patient presenting with a spinal epidural abscess and a severe neurological deficit. this is the first report of such an occurrence. | 1994 | 8150977 |
| electron microscopy of phages in serotypes of actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans, actinobacillus ureae, haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, haemophilus parainfluenzae, pasteurella haemolytica and pasteurella multocida strains were examined by transmission electron microscopy for the presence of bacteriophages. phages were detected in serotype a (suny 75) and e (uoh 1705) and in the fresh clinical isolates uoh q1243 and uoh q1247 of a. actinomycetemcomitans. phages were not found in serotype b, c an ... | 1993 | 8152840 |
| haemophilus paraphrophilus vertebral osteomyelitis. | to report the first case of haemophilus paraphrophilus vertebral osteomyelitis--the second reported case of osteomyelitis of any site caused by this organism. | 1994 | 8170429 |
| vertebral osteomyelitis caused by haemophilus aphrophilus. | | 1993 | 8223668 |
| multivariate chemosystematics demonstrate two groups of actinobacillus actinomycetemcomitans strains. | chemical analysis by us has indicated that actinobacillus actinomycetemcomitans is not a homogeneous species. the present study used chemometric methods and a multitude of chemical characters to examine this further. strains were characterized by cell sugar and fatty acid contents, lysis kinetics during edta and edta plus lysozyme exposure, methylene blue reduction, and api zym enzymatic assessment of whole cells and outer membrane vesicles/fragments. in total, 41 quantitative variables were ana ... | 1993 | 8233565 |
| killing of actinobacillus actinomycetemcomitans and haemophilus aphrophilus by human polymorphonuclear leukocytes in serum and saliva. | the ability of polymorphonuclear leukocytes from human peripheral blood to kill actinobacillus actinomycetemcomitans and haemophilus aphrophilus was examined with fresh isolates and laboratory strains from each species (5 strains within each group) under different conditions. bacterial cells were mixed with a polymorphonuclear leukocyte suspension in the presence of either active serum or heat-inactivated serum or active serum together with sterile-filtered saliva. surviving bacteria were determ ... | 1993 | 8233566 |
| isolation of haemophilus paraphrophilus from an abdominal abscess. | | 1993 | 8243492 |
| endophthalmitis caused by unusual gram-negative bacilli: three case reports and review. | endophthalmitis due to gram-negative bacilli has been associated with a high degree of vision loss. we report three cases due to the nonenteric gram-negative bacilli moraxella nonliquefaciens, haemophilus paraphrophilus, and multidrug-resistant haemophilus influenzae. the features of these cases are compared with those of other reported cases of endophthalmitis due to unusual nonenteric gram-negative bacilli. fifty-eight percent of patients had no vision in the affected eye after treatment. earl ... | 1993 | 8268350 |
| polymorphonuclear leukocyte chemiluminescence induced by actinobacillus actinomycetemcomitans and haemophilus aphrophilus in serum and saliva. | the ability of different strains of actinobacillus actinomycetemcomitans (a.a.) and haemophilus aphrophilus (h.a.) to trigger activation of an oxidative burst in human polymorphonuclear leukocytes (pmnl) was examined by measuring the luminol-amplified light emission--chemiluminescence (cl)--from these cells. bacterial cells were incubated with pmnl from one healthy subject, in the presence of either active serum, heat-inactivated serum, saliva, or saliva and active serum. in the presence of acti ... | 1993 | 8290876 |
| identification of actinobacillus actinomycetemcomitans by leukotoxin gene-specific hybridization and polymerase chain reaction assays. | eleven strains of actinobacillus actinomycetemcomitans isolated from cases of systemic infections, local abscesses, and periodontitis were identified by genetic assays using the leukotoxin gene as the target. we have developed a polymerase chain reaction (pcr) assay, based on the leukotoxin structural gene of this pathogen, which clearly identified all tested strains of a. actinomycetemcomitans and separated them from the closely related haemophilus aphrophilus as well as other bacterial species ... | 1993 | 8349764 |
| haemophilus aphrophilus discitis and vertebral osteomyelitis. | an unusual case of discitis and vertebral osteomyelitis due to haemophilus aphrophilus is described. infections due to this organism have usually responded to treatment with beta-lactam antibiotics. however, our isolate was resistant to third-generation cephalosporins which has not been reported previously in the world literature. the patient made a good clinical response to ciprofloxacin treatment. | 1995 | 8539556 |
| liver abscess caused by haemophilus paraphrophilus and streptococcus anginosus. | | 1995 | 8572383 |
| [haemophilus aphrophilus as a rare cause of bacterial spondylitis]. | we report a case of lumbar spondylitis due to haemophilus aphrophilus in a 36-year-old man with monoclonal gammopathy of undetermined significance. haemophilus aphrophilus is a gram-negative capnophilic, slow growing coccobacillus, a component of the normal human flora of the oropharynx. this organism is considered an uncommon human pathogen and has been reported in the literature in only 10 cases of bacterial spondylitis. we review the bacteriological and clinical findings, predisposing conditi ... | 1996 | 8701249 |
| polymicrobial brain abscess involving haemophilus paraphrophilus and actinomyces odontolyticus. | a case of brain abscess involving haemophilus paraphrophilus and actinomyces odontolyticus is presented. this combination of organisms has not previously been described. all brain abscess specimens should routinely be processed rapidly and cultured for a prolonged period to ensure recovery of fastidious organisms which may have implications for antibiotic therapy. | 1996 | 8761504 |
| novel fnr homologues identified in four representative oral facultative anaerobes: capnocytophaga ochracea, capnocytophaga sputigena, haemophilus aphrophilus, and actinobacillus actinomycetemcomitans. | based upon dna sequence data and positive immunochemical reactivity of expressed protein, novel homologues of the fnr family were identified in four representative oral facultative anaerobes: capnocytophaga ochracea, capnocytophaga sputigena, haemophilus aphrophilus, and actinobacillus actinomycetemcomitans. the similarity to e. coli fnr and to hlyx (itself 71% similar to e. coli fnr, while regulating expression of hemolysin operon in actinobacillus pleuropneumoniae) was estimated from the deduc ... | 1996 | 8998988 |
| myeloperoxidase isoform activities released by human neutrophils in response to dental and periodontal bacteria. | myeloperoxidase is an enzyme released by polymorphonuclear leukocytes which has been used, experimentally, as an indicator of periodontal disease activity when measured in gingival crevicular fluid. there are three myeloperoxidase isoforms: mpo i, mpo ii and mpo iii. we examined the activities of myeloperoxidase isoforms released by neutrophils in response to serum-opsonized actinobacillus actinomycetemcomitans, haemophilus aphrophilus, eikenella corrodens, capnocytophaga sputigena and streptoco ... | 1997 | 9151641 |
| rapid identification of actinobacillus actinomycetemcomitans, haemophilus aphrophilus, and haemophilus paraphrophilus by restriction enzyme analysis of pcr-amplified 16s rrna genes. | restriction enzyme analysis of pcr-amplified 16s rrna genes was used to distinguish among clinical isolates of actinobacillus actinomycetemcomitans, haemophilus aphrophilus, and haemophilus paraphrophilus which were originally identified by conventional phenotypic methods. this pcr-based method is a reliable and rapid alternative to conventional methods for identification of these bacterial species. | 1997 | 9163503 |
| spinal abscess of haemophilus paraphrophilus. a case report. | a case of paraspinal abscess formation from haemophilus paraphrophilus is presented. | 1997 | 9431611 |
| haemophilus aphrophilus bacteraemia complicated with vertebral osteomyelitis and spinal epidural abscess in a patient with liver cirrhosis. | haemophilus aphrophilus is rarely implicated as an aetiology of spinal epidural abscess. a 73-year-old woman with liver cirrhosis who developed h. aphrophilus bacteraemia complicated with vertebral osteomyelitis and spinal epidural abscess is presented. without surgical decompression, she was successfully treated with cefotaxime for 3 weeks, followed by maintenance with ciprofloxacin for another 10 weeks. the clinical features of eight previously reported cases of vertebral osteomyelitis without ... | 1997 | 9459409 |
| serum antibodies reacting with subgingival species in refractory periodontitis subjects. | the purpose of this investigation was to compare the levels of serum igg antibody to 85 subgingival species in 32 refractory periodontitis, 56 successfully treated, and 33 periodontally healthy subjects. refractory subjects showed mean full mouth attachment loss and/or >3 sites showing attachment loss >2.5 mm within 1 year after 2 treatment modalities, scaling and root planing and surgery plus systemically administered tetracycline. successfully-treated subjects showed mean attachment level gain ... | 1998 | 9696261 |
| haemophilus paraphrophilus; a rare cause of intracranial abscess. | we report a case of a 42-year-old man man who presented with neurological symptoms and was found to have an intracranial abscess. a stereotactic aspiration of the abscess yielded a pure growth of haemophilus paraphrophilus. the patient responded to treatment with cefotaxime. we postulate the mechanism of infection in this patient. | 1998 | 9733388 |
| growth differences of capnocytophaga canimorsus strains and some other fastidious organisms on various columbia-based blood agar media. | three human isolates of capnocytophaga canimorsus from blood cultures showed no growth when cultivated on sheep blood agar plates based on bbl columbia agar base, whereas the same three strains grew excellently on sheep blood agar plates based on difco columbia agar bases no. 2 or eh. two of the three strains also grew well on blood agar plates based on biomérieux columbia agar. strains that grew better on either difco or biomérieux columbia based blood agar plates were found among eikenella cor ... | 1995 | 9810657 |
| haemophilus aphrophilus meningitis complicated by hydrocephalus in an immunocompetent adult. | a case of meningitis due to an unusual organism, haemophilus aphrophilus in an adult is described. in spite of rapid clearance of the organism from the cerebrospinal fluid with intravenous cefotaxime, fever persisted over 8 days of therapy and infection was complicated by hydrocephalus. h. aphrophilus meningitis is uncommon and has not been previously reported in an adult male. | 1998 | 9861570 |
| epithelial cell invasion by actinobacillus actinomycetemcomitans strains from restriction fragment-length polymorphism groups associated with juvenile periodontitis or carrier status. | the epithelial cell invasiveness of actinobacillus actinomycetemcomitans strains of different restriction fragment-length polymorphism (rflp) groups associated with disease conversion and asymptomatic carrier status in localized juvenile periodontitis was examined. twenty clinical isolates were studied for their ability to invade kb monolayers, using the quantitative gentamicin killing assay. five isolates were found to be invasive, five were not invasive; and the other 10 did not invade better ... | 1998 | 9872109 |
| evaluation of two commercial kits and arbitrarily primed pcr for identification and differentiation of actinobacillus actinomycetemcomitans, haemophilus aphrophilus, and haemophilus paraphrophilus. | the closely related species actinobacillus actinomycetemcomitans, haemophilus aphrophilus, and haemophilus paraphrophilus are common findings in oral microbiota. the aims of this study were to evaluate the applicability of the rapid nh and api zym kits and arbitrarily primed pcr (ap-pcr) in the identification and differentiation of the three species from each other. the material included 62 clinical isolates and three reference strains of a. actinomycetemcomitans representing the 5 serotypes and ... | 1999 | 9986843 |
| dominant cross-reactive antibodies generated during the response to a variety of oral bacterial species detect phosphorylcholine. | the intraperitoneal immunization of balb/c mice with subgingival plaque from advanced periodontal pockets or with certain strains of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, actinomyces israelii, streptococcus mitis, or streptococcus oralis yielded frequently indistinguishable igm monoclonal antibodies which were reactive with antigens from a variety of oral bacteria. this study aimed to characterize the specificity of such monoclonal antibodies and the diversity of oral ba ... | 1999 | 10065949 |
| [endocarditis caused by haemophilus aphrophilus]. | | 1999 | 10217861 |
| haemophilus aphrophilus osteomyelitis after dental prophylaxis. a case report. | a 36-year-old patient who was otherwise healthy had acute osteomyelitis of the humeral shaft develop after routine prophylactic dental cleaning and ultrasonic scaling. haemophilus aphrophilus grew on cultures of material obtained during biopsy of the humerus, and pathologic examination confirmed the diagnosis of acute osteomyelitis. haemophilus aphrophilus, a fastidious gram negative bacillus, is part of the normal oral flora and is a rare pathogen. osteomyelitis caused by haemophilus aphrophilu ... | 1999 | 10379323 |
| microtubules are associated with intracellular movement and spread of the periodontopathogen actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans suny 465, the invasion prototype strain, enters epithelial cells by an actin-dependent mechanism, escapes from the host cell vacuole, and spreads intracellularly and to adjacent epithelial cells via intercellular protrusions. internalized organisms also egress from host cells into the assay medium via protrusions that are associated with just a single epithelial cell. here we demonstrate that agents which inhibit microtubule polymerization (e.g., colchicine) ... | 1999 | 10569770 |
| recurrent cervical lymphadenitis caused by haemophilus aphrophilus. | | 2000 | 10722471 |
| serological relationship of some v-factor dependent pasteurellaceae (haemophilus sp.) from guineapigs and rabbits. | culture of guinea pig and rabbit respiratory tracts for bacteria using x- (haemin) and v- (nad) factor in agar media detected infection by v-factor dependent pasteurellaceae (haemophilus sp.) in three colonies of guinea pigs and a group of rabbits. the 12 haemophilus strains comprised three api nh codes classed as haemophilus parainfluenzae and two codes classed as haemophilus aphrophilus/paraphrophilus. six cell wall lipid profiles were detected, but these were not related to api nh codes. both ... | 1999 | 10759399 |
| checkerboard dna-dna hybridization analysis of endodontic infections. | the purpose of this investigation was to examine the microbiota of infected root canals by using a molecular genetic method. | 2000 | 10846131 |
| [a case of pyogenic vertebral osteomyelitis caused by haemophilus aphrophilus]. | on aug. 3, 1999, a 73-year-old male was admitted to our hospital with the chief complaint of pain in the neck, high fever, and numbness in the arm. mri of the cervix showed high intensity at the c3/c4 disc space. laboratory data showed several signs of inflammation. haemophilus aphrophilus was detected from the specimen of the disc space, and the diagnosis of pyogenic vertebral osteomyelitis caused by h. aphrophilus was made. after the identification of h. aphrophilus, antibiotic therapy with ce ... | 2000 | 11193560 |
| possible misidentification of haemophilus aphrophilus as pasteurella gallinarum. | | 2001 | 11247724 |
| it could only happen to a doctor--haemophilus aphrophilus septicaemia complicated by a prevertebral infection after dental work. | a 53 year old man presented with severe neck pain and a flu-like illness; he had recently returned from sri lanka and had had dental treatment six days before illness onset. blood culture showed infection by haemophilus aphrophilus. magnetic resonance imaging was performed and exploratory surgery undertaken. the prevertebral cervical fascia was inflamed but no abscess identified. he was treated with antibiotics and made an uneventful recovery. | 2001 | 11264493 |
| vertebral infections caused by haemophilus aphrophilus: case report and review. | to review in detail clinical presentation, bacteriologic findings, associated conditions and treatment of haemophilus aphrophilus vertebral osteomyelitis and to compare them to a case we report herein. | 2001 | 11318807 |
| phosphorylcholine-dependent cross-reactivity between dental plaque bacteria and oxidized low-density lipoproteins. | antibodies reactive with phosphorylcholine (pc) are ubiquitous in human sera, but the antigens stimulating their production and their function are not clear. previous studies have shown that a significant proportion of dental plaque bacteria contain pc as determined by reactivity with pc-specific mouse myeloma proteins and monoclonal antibodies. additionally, serum antibody concentrations of immunoglobulin (igg) g anti-pc are higher in sera of individuals who have experienced periodontal attachm ... | 2001 | 11598029 |
| haemophilus aphrophilus bleb infection after a mitomycin trabeculectomy. | haemophilus aphrophilus is a rare cause of ocular infection. it has been reported once as a cause of late-onset endophthalmitis in a patient with an inadvertent bleb after cataract surgery. we present a case of haemophilus aphrophilus bleb infection after a mitomycin trabeculectomy. | 1995 | 11980080 |
| haemophilus aphrophilus endocarditis after tongue piercing. | piercing invades subcutaneous areas and has a high potential for infectious complications. the number of case reports of endocarditis associated with piercing is increasing. we studied a 25-year-old man with a pierced tongue, who arrived at memorial health university medical center with fever, chills, rigors, and shortness of breath of 6 days' duration and had an aortic valvuloplasty for correction of congenital aortic stenosis. | 2002 | 12141972 |
| images in clinical medicine. septic peripheral embolization from haemophilus aphrophilus endocarditis. | | 2002 | 12226152 |
| biofilm dispersal of neisseria subflava and other phylogenetically diverse oral bacteria. | polystyrene petri dishes containing liquid medium were inoculated with single-cell suspensions of a fresh clinical isolate of neisseria subflava and were incubated under conditions of low vibration. n. subflava colonies grew firmly attached to the surface of the dish, while the broth remained clear. growing colonies released cells into the medium, resulting in the appearance of 10(2) to 10(4) small satellite colonies attached to the surface of the dish in an area adjacent to each mature colony a ... | 2002 | 12324342 |
| haemophilus aphrophilus brain abscess: a case report. | haemophilus aphrophilus infection is rare, and the organism is infrequently implicated in serious infection. we report a case of a 61-year-old patient who experienced left hemiparesis with dizziness. computed tomography of the brain demonstrated a lesion with ring enhancement in the right frontotemporal region. craniotomy was performed, abscess was drained, and h. aphrophilus was isolated. following the surgical procedure and further antibiotic treatment, the patient recovered completely. | 2002 | 12380792 |
| infective endocarditis due to haemophilus aphrophilus treated with levofloxacin. | | 2002 | 12505130 |
| haemophilus paraphrophilus prosthetic valve endocarditis. | we report a case of prosthetic, aortic valve, infective endocarditis caused by haemophilus paraphrophilus. there are no other cases described in the available literature where this microorganism has caused prosthetic valve endocarditis and no other case reported involving only the aortic valve. | 2003 | 12643871 |
| actinobacillus actinomycetemcomitans and haemophilus aphrophilus in systemic and nonoral infections in finland. | the oral cavity is the ecological niche for actinobacillus actinomycetemcomitans and haemophilus aphrophilus, but occasionally they cause severe nonoral infections. in this study we present 20 systemic or nonoral infections due to a. actinomycetemcomitans and h. aphrophilus, comprising all isolates of these species forwarded to and stored in finnish reference laboratories during the years 1988-2000. the time from specimen collection to correct species identification was 9.3 days for a. actinomyc ... | 2003 | 12969021 |
| [bacterial endocarditis due to haemophilus aphrophilus]. | | 1962 | 13996319 |
| brain abscess due to haemophilus aphrophilus. | | 1964 | 14195761 |
| infection with haemophilus aphrophilus: brain abscess. | | 1965 | 14271378 |
| [bacteremia in 2 hospitals in rosario, argentina]. | bacteremia is associated with high morbidity and mortality. nosocomial bacteremia is associated with an attributable mortality of 35%. in order to characterize the etiological agent of bacteremia cases during two time periods, 6605 patients were studied as follows: 1) from 1980 to 1982 (n = 2401) and 2) from 1988 to 2000 (n = 4204). a total of 596 cases of bacteremia were detected. the most frequent agents were staphylococcus aureus, negative coagulase (cns) staphylococcus, klebsiella pneumoniae ... | 2003 | 14587380 |
| [piercing and tattoos in patients with congenital heart disease -- is it a problem?]. | piercing and tattooing enjoy widespread popularity in modern society. patients with congenital heart disease are at elevated risk for infective endocarditis. however, it is not yet known whether piercing and tattooing are dangerous for these patients.a search of the literature provided 10 published cases of infective endocarditis after piercing or tattooing. affected patients were adolescents or young adults ranging in age from 13 to 30 years (5 male, 5 female). four of the patients had a known ... | 2004 | 15338148 |
| [solitary abscessing osteomyelitis of the mandibular condyle. a rarity]. | we report on a rare case of secondary chronic osteomyelitis of the left condyle. haemophilus aphrophilus could be isolated from the abscess material. the condyle was resected and reconstructed with an endoprosthesis in the same operation. | 2005 | 15965774 |
| [shoulder arthritis due to haemophilus aphrophilus]. | we report a case of shoulder arthritis due to haemophilus aphrophilus. the patient, a 56 year-old woman, was immunocompetent. she presented with a septic arthritis of the left shoulder without portal of entry. a synovial fluid sample was cultured and positive for a gram-negative bacillus after 8 days. it was identified as haemophilus aphrophilus, in the haccek group, by pcr arn 16s. we did not find any associated endocarditis. the patient recovered. as far as we know, this is only the 5th report ... | 2005 | 15982845 |
| a case of haemophilus aphrophilus and mobiluncus mulieris hepatic abscess. | | 2005 | 16038743 |
| clinical characteristics of invasive haemophilus aphrophilus infections. | haemophilus aphrophilus, an oral fastidious gram-negative commensal with low pathogenicity, is a member of the hacek group (h. aphrophilus, h. paraphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella spp.), and a rare cause of human infections. we reviewed the characteristics of 8 cases of h. aphrophilus infections diagnosed in our hospital from 1990-2003, and an additional 20 cases identified from the medline database, from 1990 to 2003. t ... | 2005 | 16118675 |
| haemophilus aphrophilus brain abscess in the first decade. | this report presents a case of brain abscess due to haemophilus aphrophilus in a six-year old boy. he was admitted to our hospital suffering from left-sided weakness. the initial radiological diagnosis was an intracranial abscess. purulent material was obtained by puncturing the subcortical lesion and the sample was cultured on conventional media. h. aphrophilus was isolated in pure culture, identified according to conventional methods and confirmed by becton dickinson laboratory (bbl) crystal s ... | 2005 | 16327124 |
| cardiobacterium hominis endocarditis: two cases and a review of the literature. | cardiobacterium hominis, a member of the hacek group (haemophilus parainfluenzae, haemophilus aphrophilus, and haemophilus paraphrophilus, actinobacillus actinomycetemcomitans, c. hominis, eikenella corrodens, and kingella species), is a rare cause of endocarditis. there are 61 reported cases of c. hominis infective endocarditis in the english-language literature, 15 of which involved prosthetic valve endocarditis. there is one reported case of c. hominis after upper endoscopy and none reported ... | 2006 | 16955250 |
| reclassification of actinobacillus actinomycetemcomitans, haemophilus aphrophilus, haemophilus paraphrophilus and haemophilus segnis as aggregatibacter actinomycetemcomitans gen. nov., comb. nov., aggregatibacter aphrophilus comb. nov. and aggregatibacter segnis comb. nov., and emended description of aggregatibacter aphrophilus to include v factor-dependent and v factor-independent isolates. | the aim of this study was to reinvestigate the relationships and the generic affiliations of the species actinobacillus actinomycetemcomitans, haemophilus aphrophilus, haemophilus paraphrophilus and haemophilus segnis. the nicotinamide phosphoribosyltransferase gene (nadv) conferring v factor-independent growth was identified in haemophilus aphrophilus. the gene encodes a polypeptide of 462 amino acids that shows 74.5 % amino acid sequence identity to the corresponding enzyme from actinobacillus ... | 2006 | 16957111 |
| the soda gene as a target for phylogenetic dissection of the genus haemophilus and accurate identification of human clinical isolates. | the genus haemophilus constitutes a heterogeneous group of pasteurellaceae species, and conventional identification of isolates other than haemophilus influenzae and haemophilus parainfluenzae is often challenging. here, simple colony-pcr and sequencing assays with the same pair of degenerate primers were used to characterize a 449- to 458-bp fragment (soda(int)) internal to the soda gene encoding the manganese-dependent superoxide dismutase in type strains of all 15 haemophilus species and acti ... | 2006 | 17049306 |