| molecular cloning and in vitro expression of a cdna clone for human cellular tumor antigen p53. | three clones for the human tumor antigen p53 were isolated from a cdna library prepared from a431 cells. one of these clones, pr4-2, contains the entire coding region for human p53. this clone directs the synthesis of a polypeptide with the correct molecular weight and immunological epitopes of an authentic p53 molecule in an in vitro transcription-translation reaction. although the pr4-2 clone contains the coding region for p53, it is not a full-length copy of the human p53 mrna. northern analy ... | 1985 | 3894933 |
| gonadotropin-releasing hormone within the organum vasculosum lamina terminalis in the ovariectomized, estrogen/progesterone-treated rat: a quantitative immunocytochemical study using image analysis. | no specific function has been ascribed to the high gonadotropin-releasing hormone (gnrh) content of the organum vasculosum lamina terminalis (ovlt). the objective of this study was to determine whether levels of gnrh within the ovlt are altered during cyclic gonadotropin secretion. gnrh levels were determined at various times during an estrogen/progesterone (e/p)-induced lh surge. groups of e/p and sesame oil-treated animals were decapitated at 12.00 h, 14.00 h, 16.00 h, 18.00 h, and 22.00 h fol ... | 1985 | 3896396 |
| identification of hydroxy fatty acids in aeromonas hydrophila, aeromonas sobria, and aeromonas caviae. | gas-liquid chromatographic analysis of fatty acid methyl esters obtained from clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae revealed the presence of two hydroxy fatty acid species, 3-oh 12:0 and 3-oh 14:0. | 1985 | 3934208 |
| successful treatment of post-mitral valve annuloplasty aspergillus flavus endocarditis. | aspergillus endocarditis is associated with a very high mortality. of approximately 67 cases reported in the english language literature, there have been only two known survivors. this report describes a patient with aspergillus flavus endocarditis after mitral valve annuloplasty who recovered with combined surgical and antifungal therapy. this is the first successfully treated case due to a. flavus and the first involving an annuloplasty ring. | 1985 | 3934969 |
| effects of 0.5 ppm ozone on glycoprotein secretion, ion and water fluxes in sheep trachea. | we studied the effects of ozone (o3) exposure on airway mucus secretion. sheep were exposed in vivo to 0.5 ppm o3, 4 h/day for 2 days (acute, n = 6), 6 wks (chronic, n = 6) or 6 wks + 1 wk recovery (chronic + recovery, n = 6). secretion of glycoproteins (radiolabeled with 35so4 and [3h]threonine), and transepithelial fluxes of cl-, na+ and water were subsequently measured in tracheal tissues in vitro, and were compared with values from control, unexposed sheep (n = 8). acute o3 exposure increase ... | 1986 | 3957842 |
| prevalence, species differentiation, and toxigenicity of aeromonas strains in cases of childhood gastroenteritis and in controls. | in a 1-year period (january to december 1984), aeromonas strains were isolated from feces of 21 of 561 (3.7%) children with gastroenteritis and from 12 of 576 (2.1%) children without intestinal disturbances (controls). the difference between the two isolation rates was not significant (x2 = 2.2; p greater than 0.05). in five cases of illness other intestinal pathogens were isolated together with aeromonas in the same stool sample. a total of 39 aeromonas strains were detected since in some cases ... | 1986 | 3958147 |
| immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria. | purified catabolic ornithine carbamoyltransferase of pseudomonas putida and anabolic ornithine carbamoyltransferase (argf product) of escherichia coli k-12 were used to prepare antisera. the two specific antisera gave heterologous cross-reactions of various intensities with bacterial catabolic ornithine carbamoyltransferases formed by pseudomonas and representative organisms of other bacterial genera. the immunological cross-reactivity observed only between the catabolic ornithine carbamoyltrans ... | 1985 | 3968036 |
| metastatic nontraumatic clostridium septicum osteomyelitis. | nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated. | 1985 | 3980945 |
| comparative in vitro activities of selected antimicrobial agents against aeromonas species and plesiomonas shigelloides. | the in vitro antimicrobial susceptibilities of fecal isolates of aeromonas caviae, aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were tested by agar dilution. this in vitro study suggested that ciprofloxacin, enoxacin, norfloxacin, tetracycline, and trimethoprim-sulfamethoxazole might be effective oral agents for treatment of diarrhea involving aeromonas spp. or p. shigelloides. | 1985 | 4004196 |
| [regulation of heliomycin biosynthesis by the carbon source]. | the effect of different carbon sources on the biosynthesis of heliomycin by streptomyces olivocinereus 11-98 was studied. inverse relationship between the culture growth rate and the biosynthesis of heliomycin in the media with different sugars was shown. arabinose and glucose provided the highest culture growth rate, while the levels of the antibiotic biosynthesis were the lowest. with the use of lactose the culture growth rate was the lowest and the antibiotic biosynthesis level was the highes ... | 1985 | 4026244 |
| [probability in the evaluation of the reliability of bactericidal effects]. | | 1985 | 4029632 |
| in vitro susceptibilities of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae to 22 antimicrobial agents. | mics of 22 antimicrobial agents for 60 strains of three aeromonas species were determined by a microdilution method. the newer cephalosporins such as moxalactam, cefotaxime, and cefoperazone, the aminoglycosides, and chloramphenicol, tetracycline, nitrofurantoin, and trimethoprim-sulfamethoxazole inhibited most of the strains studied. within the genus, a. hydrophila was more resistant than either a. caviae or a. sobria to the antibiotics tested. | 1985 | 4037775 |
| comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae. | sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ... | 1985 | 4056003 |
| aeromonas caviae: an enteric pathogen? | of the aeromonas spp. isolated routinely in our laboratory from human feces, about two thirds represent aeromonas caviae. in contrast to aeromonas hydrophila and aeromonas sobria, this species has been considered to be of little enteropathogenic significance due to the absence of known virulence factors. the clinical data presented here suggest a clinical significance of a. caviae, at least in some cases. as yet nothing is known about the pathogenic mechanisms involved. | 1985 | 4066046 |
| classically conditioned hyperglycemia in the obese mouse. | the obese (c57bl/6j ob/ob) mouse is a commonly used animal model of non-insulin-dependent diabetes mellitus. it has recently been demonstrated that this mouse is not consistently hyperglycemic, however, unless it is subjected to environmental stress. in the present study, hyperglycemia in obese mice was induced by classical conditioning. obese diabetic mice and lean control animals were exposed to shaking stress. all animals developed hyperglycemia in response to shaking. to demonstrate classica ... | 1985 | 4070525 |
| electrophoretic types of transaldolase, transketolase, and other enzymes in bifidobacteria. | | 1974 | 4136709 |
| interrelations of the search for naturally-occurring goitrogens to the treatment of thyrotoxicosis. | | 1974 | 4141488 |
| effect of salicylates on human platelets. | | 1968 | 4173780 |
| biosynthesis of peptidoglycan in the one million molecular weight range by membrane preparations from bacillus megaterium. | | 1974 | 4211096 |
| [pharmacologic studies of radix puerariae. i. its effect on dog arterial pressure, vascular reactivity, and cerebral and peripheral circulation]. | | 1974 | 4211555 |
| [influence of temperature on the development of experimental infection of the newborn mouse by coxsackie virus]. | | 1966 | 4222899 |
| [studies concerning the demonstration of sessile antibodies in carp (cyprinus carpio l.). ii. demonstration of sessile antibodies to aeromonas punctata in carp adapted under natural conditions]. | | 1969 | 4239396 |
| occurrence and development of experimental silicosis under the effect of vitamin c and rutascorbin. | | 1969 | 4245171 |
| studies on the structure of the mitochondrial membranes. | | 1970 | 4245917 |
| [study of the metabolism of chondroitin sulfate in the cartilage of vitamin a deficient chickens using labelled precursors]. | | 1970 | 4249424 |
| the response of the pituitary-adrenal system to a stressful stimulus: the effect of conditioning and pentobarbitone treatment. | | 1965 | 4284301 |
| inhibitory activity of tannic acid and flavonols on the infectivity of herpesvirus hominis and herpesvirus suis. | | 1965 | 4286615 |
| deficiencies of chromatophore proteins in some mutants of rhodopseudomonas spheroides with altered carotenoids. | chromatophore proteins of a wild type and three mutant strains of rhodopseudomonas spheroides were examined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the mutants consisted of a green and a blue-green one, whose phenotypes were essentially the same as those of known mutants, and a brown one, which may be a double mutant and represents a new phenotype. wild-type chromatophores contained at least six major and seven minor protein bands, with molecular weights ... | 1971 | 4323296 |
| [a field trial on the effect of vitamin d 3 against parturient paresis]. | | 1971 | 4334605 |
| d-fucose metabolism in a pseudomonad. i. oxidation of d-fucose to d-fucono- -lactone by a d-aldohexose dehydrogenase. | | 1972 | 4335867 |
| origin and central pathways of crossed inhibitory effects of afferents from the masseteric muscle on the masseteric motoneuron of the cat. | | 1973 | 4352016 |
| salmonella typhimurium hisg46 (r-utrecht): possible use in screening mutagens and carcinogens. | salmonella typhimurium lt2 hisg46 becomes a more sensitive strain for assaying mutagens and carcinogens when it carries the resistance transfer factor r-utrecht. | 1973 | 4358393 |
| antimicrobial therapy in experimental pseudomonas infection. | | 1974 | 4372741 |
| simple method of 24-hour spleen preservation: further studies relating to hemophilia. | | 1971 | 4398505 |
| simple method of 24-hour spleen preservation: further studies relating to hemophilia. | | 1971 | 4398505 |
| the multiplication of main drain virus in two species of culicoides (diptera, ceratopogonidae). | | 1974 | 4441256 |
| effect of oral chenodeoxycholic acid on bile acid kinetics and biliary lipid composition in women with cholelithiasis. | bile acid kinetics and biliary lipid composition were characterized in six women with gallstones before and after 6 mo of oral therapy with chenodeoxycholic acid, an agent that induces dissolution of cholesterol gallstones in man. over a dosage range of 1-4 g/day, absorption varied from 0.8 to 2.3 g/day. the chenodeoxycholic acid pool expanded two-to sixfold, and bile became composed predominantly (> 90%) of chenodeoxycholic acid conjugated chiefly with glycine. cholic acid and deoxycholic acid ... | 1973 | 4583981 |
| technique for chronic head restraint and electrophysiologic recording in the awake rhesus monkey. | | 1974 | 4612566 |
| [oral and parenteral, active immunization of carp against aeromonas punctata]. | | 1972 | 4665867 |
| giardia intestinalis. a prospective investigation of the prevalence in denmark. | | 1973 | 4697076 |
| depression of isolated heart muscle by bacterial endotoxin. | | 1973 | 4711286 |
| hepatitis b antigens: separation of two populations differing in particle size distribution. | | 1973 | 4733164 |
| [nitrogen excretion in growing poultry on a nitrogen-free diet]. | | 1973 | 4750714 |
| [the formation of subjective evaluation of time in man]. | | 1973 | 4772236 |
| surgical reconstruction for collapsed tracheal rings. | | 1973 | 4785759 |
| a chromosomal break and partial deletion of a number 9 chromosome. | | 1973 | 4792221 |
| [activity of glycine amidinotransferase and its isoforms in dog kidney tissue normally and in acute renal insufficiency]. | | 1974 | 4823750 |
| effect of anaerobiosis on the concentrations of demethylmenaquinone, menaquinone and ubiquinone in escherichia freundii, proteus mirabilis and aeromonas punctata. | | 1968 | 4875411 |
| the nature, intergeneric distribution and biosynthesis of isoprenoid quinones and phenols in gram-negative bacteria. | 1. twenty-two aerobically grown gram-negative bacteria were analysed for demethylmenaquinones, menaquinones, 2-polyprenylphenols, 6-methoxy-2-polyprenylphenols and ubiquinones. 2. all the eight enterobacteria and both the two facultative organisms (aeromonas punctata and aeromonas hydrophila) examined contain all the compounds listed above. the principal homologues are octaprenyl; in addition lower (down to tri- or tetra-prenyl for the 2-polyprenylphenols) and sometimes higher homologues are als ... | 1969 | 4886765 |
| comparative study of isoenzyme formation of bacterial beta-galactosidase. | the enzyme beta-galactosidase was studied in crude extracts of escherichia coli 3300, e. coli grown on a selenium and sulfur medium, salmonella typhimurium f-lac, serratia marcescens f-lac, s. marcescens p-lac, proteus mirabilis f-lac, p. mirabilis p-lac, aeromonas formicans, and streptococcus lactis. the isoenzymes could be demonstrated by an alternative histochemical technique. different isoenzyme patterns were found to be determined by the beta-galactosidase structural gene and not by the cyt ... | 1970 | 4908684 |
| osteopetrosis virus growth in the kidney of infected chickens. | | 1966 | 4960239 |
| comparative allostery of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthetase as a molecular basis for classification. | the allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. the implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organisms, sporosarcina ureae and aeromonas formicans, the taxonomic positions of which have been historically controversial. s. ureae has characteristics of both bacillaceae and microco ... | 1970 | 4985590 |
| localization of function within the dorsolateral prefrontal cortex of the rhesus monkey. | | 1970 | 4987453 |
| the reaction of anopheles annulipes larvae to infection by aeromonas punctata. | | 1972 | 5014911 |
| experimental infection of beagle dogs with coxsackievirus type b1. | | 1971 | 5110117 |
| experimental infection of beagle dogs with coxsackievirus type b1. | | 1971 | 5110117 |
| [study of the antigenic properties of aeromonas punctata strains]. | | 1971 | 5140664 |
| [the ultrastructure of aeromonas punctata bacteria]. | | 1971 | 5152580 |
| [blood changes in the carp following infection with aeromonas punctata]. | | 1971 | 5158662 |
| purification and characterization of the beta-galactosidase of aeromonas formicans. | rohlfing, s. r. (western reserve university, cleveland, ohio), and i. p. crawford. purification and characterization of the beta-galactosidase of aeromonas formicans. j. bacteriol. 91:1085-1097. 1966.-the beta-galactosidase of aeromonas formicans was purified by diethylaminoethyl cellulose chromatography and gel filtration on sephadex g-200. the properties of the enzyme molecule were compared with purified beta-galactosidase from escherichia coli. the sedimentation coefficients and electrophoret ... | 1966 | 5326700 |
| the natural relationships of aeromonas formicans. | | 1967 | 5602475 |
| [study of the properties of the toxin of aeromonas punctata]. | | 1968 | 5720508 |
| [study of flagellation in aeromonas punctata]. | | 1966 | 6002909 |
| morphological and biochemical characteristics of aeromonas punctata (hydrophila, liquefaciens) isolated from human sources. | the isolation of aeromonas punctata (hydrophila, liquefaciens) from feces, throat, and sputum cultures is presented as further evidence that aeromonads are found in man. morphological and biochemical studies of these strains indicate that the chief differences between the aeromonads and physiologically similar members of the enterobacteriaceae are found in the polar arrangement of the flagella and in the production of oxidase by the former. the oxidase test should be performed on all paracolon-l ... | 1967 | 6029837 |
| enzymes of arginine utilization and their formation in aeromonas formicans ncib 9232. | in aeromonas formicans two inducible catabolic pathways of l-arginine have been characterized. the arginine decarboxylase is induced by arginine which also induces the three enzymes of the arginine deiminase pathway but only in stress conditions such as a shift from aerobic growth conditions to very low oxygen tension. addition of glucose to medium containing arginine leads to repression of the enzymes involved in the arginine deiminase pathway while exogenous camp prevents that repression of en ... | 1982 | 6303241 |
| [study of various application methods for active immunization of fishes with aeromonas salmonicida, aeromonas punctata and vibrio anguillarum. 1. immunization with aeromonas salmonicida strain 314]. | | 1983 | 6352234 |
| purification and properties of amino acid racemase from aeromonas punctata subsp. caviae. | an amino acid racemase, which occurs in the cytoplasmic fraction of aeromonas punctata subsp. caviae, has been purified to homogeneity by the criteria of electrophoresis and ultracentrifugation. the enzyme has a molecular weight of about 80,000 and consists of two subunits identical in molecular weight (about 40,000). the enzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme, and exhibits absorption maxima at 280 nm and 420 nm. the holoenzyme is resolved by dialysis against hydroxyla ... | 1984 | 6427786 |
| value of blood agar for primary plating and clinical implication of simultaneous isolation of aeromonas hydrophila and aeromonas caviae from a patient with gastroenteritis. | the simultaneous recovery of aeromonas hydrophila and aeromonas caviae from the stool of a 49-year-old woman with watery diarrhea was facilitated through the use of a blood agar medium which detected the hemolytic capability of a. hydrophila. in vitro phenotypic tests support the conclusion that only the a. hydrophila isolate was clinically significant. | 1984 | 6520231 |
| biotyping of aeromonas isolates as a correlate to delineating a species-associated disease spectrum. | a group of 147 aeromonas isolates from diverse clinical and environmental sources was subjected to the biotyping scheme of popoff and veron. of the 147 isolates biotyped, 137 (93%) could be identified, with aeromonas hydrophila predominating (48%) and equal percentages (25 to 27%) of the other two species (aeromonas sobria and aeromonas caviae). a number of additional biochemical properties were found to be significantly associated with one or more of these three species. these included lysine d ... | 1984 | 6690467 |
| biochemical characteristics of enterotoxigenic aeromonas spp. | biotypes of aeromonas spp. correlated well with enterotoxin production in a study of 174 strains. using biochemical characteristics determined by conventional methods and multitest systems, we correctly classified 93% of the strains with regard to enterotoxin production. most of the enterotoxigenic strains were voges-proskauer (vp) positive and did not hydrolyze arabinose, but vp-positive strains which hydrolyzed arabinose were mainly non-enterotoxigenic. aeromonas punctata subsp. caviae, which ... | 1982 | 6764776 |
| [studies of lipase and antilipase on different strains of the genera serratia, aeromonas and vibrio (author's transl)]. | lipase and antilipase were studied in the following genera: serratia, aeromonas and vibrio. numerous comparative investigations showed that the jipase of serratia marcescens is not of a uniform antigenic structure as not all antilipase-sera of serratia marcescens have the ability to neutralize the lipase of all studied strains of this species. cross-reactions were seen between the genera serratia and aeromonas. a special reaction of the antilipase sera of aeromonas hydrophila and aeromonas punct ... | 1980 | 6999789 |
| enterotoxigenicity of aeromonas strains in suckling mice. | the enterotoxigenicity of 170 aeromonas strains isolated from different sources (food poisoning, random food sampling, water, faeces) was examined by the suckling mouse test. the strains were grown on syncaye culture medium covered with sterilized membrane for kiil-kidney. the culture supernatants were inoculated orally. ileal loop dilatation was compared to that produced by the international standard enterotoxic escherichia coli b7a (o148 : h28) and b2c (o6 : h16) strains. of the 87 aeromonas h ... | 1980 | 7415872 |
| chronic diarrhea due to a single strain of aeromonas caviae. | over a period of 17 months, aeromonas caviae was cultured 15 times as the sole enteropathogen from the feces of a man who had developed chronic diarrhea after traveling to turkey. determination of rrna gene restriction patterns confirmed that the seven isolates of aeromonas caviae studied were identical (hybridization group [hg]4). after therapy with ciprofloxacin for four weeks, the patient was culture negative for the original isolate, and six months later a novel strain of aeromonas media wit ... | 1995 | 7537217 |
| cloning and primary structure of the chia gene from aeromonas caviae. | the chia gene from aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase a of serratia marcescens. expression in escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane. | 1995 | 7608101 |
| aeromonas caviae exhibits aggregative adherence to hep-2 cells. | | 1994 | 7880270 |
| molluscan immunobiology: isolation of an aeromonas formicans which escapes the internal defense system of helix pomatia. | bacterial infections are surprisingly rare in wild populations of gastropod molluscs. a strain of aeromonas formicans, which has now been isolated, kills helix pomatia, a land snail, when injected at doses greater than 10(6) viable cells g-1 snail weight. koch's postulates were fulfilled with this organism. initial clearance of aeromonas from the circulation was followed within hours by a bacteremia; since neither culture supernatants, killed cells nor cell extracts killed helix, there is eviden ... | 1982 | 7160512 |
| el fenómeno suicida en cepas de aeromonas mesófilas aisladas de muestras clínicas [the suicide phenomenon in strains of mesophilic aeromonas isolated from clinical specimens]. | a study was carried out to determine the occurrence of the suicide phenomenon in aeromonas spp strains, isolated from clinical samples, and to establish its relationship with the clinical manifestations of diarrheal diseases. 23 strains were studied: 10, of aeromonas sobria; 7, of aeromonas hydrophila; and 6, of aeromonas caviae. all suicidal strains were isolated from patients with acute diarrheal disease. 3 out of 8, isolated from non-diarrheic feces, showed an intermediate phenotypic profile. ... | 1993 | 7984811 |
| [the identification of aeromonas species isolated in cuba]. | 100 aeromonas strains, isolated from children under 5 years with acute diarrheal diseases from various health centers of the country, were studied from january to july, 1990. using the janda-modified popoff's and veron's model, 63% of the strains were identified in species through primary tests, and 100% of the other 37% were identified in aeromonas sobria, aeromonas hydrophila, and aeromonas caviae, using also supplementary tests. | 1993 | 7984818 |
| the vertebrate peptide antibiotics dermaseptins have overlapping structural features but target specific microorganisms. | the physiological significance of the occurrence of sequence similar antimicrobial peptides in frog skin, as the bombinins in bombina, the magainins in xenopus, and the dermaseptins in phyllomedusa, is a major unanswered question. dermaseptins s1, s2, s3, s4, and s5, a family of cationic (lysine-rich), amphipathic antifungal peptides of 28-34 residues were thus synthesized, purified to homogeneity, and evaluated for their growth-inhibition activity in vitro against various pathogenic microorgani ... | 1994 | 7989335 |
| aeromonas peritonitis. | five new cases of peritonitis caused by aeromonas species are reported, and 29 others described in the literature are reviewed. males predominated (71%), and the mean age was 56.9 years. acquisition was nosocomial in 20% of the cases. all patients except one (3%) had significant underlying diseases; 73% had chronic hepatic disease, 15% had chronic renal failure (treated with chronic ambulatory peritoneal dialysis [capd]), and 9% had an intestinal perforation. symptoms were similar to those of pe ... | 1994 | 8054435 |
| characterisation of haemolytic activity from aeromonas caviae. | aeromonas caviae, an enteropathogen associated with gastroenteritis, displays several virulence characteristics. studies on the kinetics of growth of a. caviae and expression of beta-haemolytic toxin revealed that a. caviae produced maximum haemolytic activity extracellularly during the stationary phase. preliminary studies on the properties of a. caviae haemolysin suggested that divalent cations (mg2+ and ca2+) and thiol compounds, dithiothreitol and mercaptoethanol enhanced the haemolytic acti ... | 1994 | 8150003 |
| characterization of mesophilic aeromonas from clinical specimens by computerized analysis of sds-page protein profiles and by enzymatic activity. | mesophilic aeromonas (aeromonas hydrophila, aeromonas sobria, aeromonas caviae) have recently been considered important aetiological agents of human diseases, mainly gastrointestinal infections. although several findings have pointed out the significance of this group of microorganisms as enteric pathogens and suggested the presence of virulence factors, epidemiological and clinical studies are limited by the difficulty of correctly identifying mesophilic aeromonas at the species level. sds-page ... | 1993 | 8264423 |
| factors influencing beta-galactosidase activity of aeromonas caviae. | aeromonas caviae, often reported to be associated with diarrhoeal patients, elaborates several virulence factors as well as catabolic enzymes such as xylanase and beta-galactosidase. studies on the kinetics of growth of a. caviae and synthesis of beta-galactosidase suggested that the activity was cell associated and reached a peak during the late logarithmic phase of growth. the optimum ph for beta-galactosidase activity was 7.0 and required ca2+ and glutathione for enhancement of its activity; ... | 1994 | 7932108 |
| incidence of motile aeromonas spp. in foods. | a total of 80 food samples were purchased from local retail consumer shops and examined for the presence of motile aeromonas spp. of the food categories tested, poultry had the highest incidence, with 100% positive. this was followed by lamb samples, with 60% positive. raw milk and cheese samples had very low incidence (20%). no motile aeromonas spp. were found in pre-prepared salads. shellfish, fish, pork and beef samples had incidences of 40%. most of the strains isolated were aeromonas hydrop ... | 1994 | 7873101 |
| tolerance to acid in ph 5.0-grown organisms of potentially pathogenic gram-negative bacteria. | a wide range of potentially pathogenic species of gram-negative bacteria were far more resistant to extreme acidity (ph 2.0-3.5) when cultured at ph 5.0 (habituated to acid) than after ph 7.0 culture. the differences were particularly great for citrobacter spp., enterobacter spp., klebsiella spp. and for vibrio parahaemolyticus; substantial habituation was also observed for proteus mirabilis and aeromonas formicans but the effect was less marked for serratia marcescens and acinetobacter calcoace ... | 1995 | 8554762 |
| prevention of suicide phenomenon in aeromonads. | clinical isolates of aeromonas (13 aeromonas caviae), 8 aeromonas hydrophila, 3 aeromonas spp., and 2 aeromonas media recovered from diarrheal feces of children were submitted to the suicide phenomenon test and investigated at intervals of 24 h for up to 120 h. the same isolates were also stimulated by repeated passages in broth for ten days before the test. after the bacterial stimulus, a decreases in the number of aeromonads with suicidal capacity was observed. the suicide phenomenon was expre ... | 1996 | 8793406 |
| diarrheal and environmental isolates of aeromonas spp. produce a toxin similar to shiga-like toxin 1. | diarrheal and environmental isolates of 39 strains of aeromonas spp. were studied for detection of virulence factors. although these 39 strains did not produce either heat-labile or heat-stable enterotoxins, culture filtrates of 31 strains produced cytopathic effects on vero cells. among these, culture filtrates of three strains of aeromonas hydrophila and one strain of aeromonas caviae could be neutralized by escherichia coli o157:h7 shiga-like toxin 1 antiserum. a single band of plasmid dna of ... | 1996 | 8857271 |
| proteolytic activity of aeromonas caviae. | aeromonas strains produce a variety of virulence factors including proteases. studies on the kinetics of growth of aeromonas caviae nrrl b-966 and its proteases suggest that the proteolytic activities are produced throughout the growth phase, with peak level occurring at stationary phase. a. caviae synthesize both intracellular and extracellular proteases with the latter account for major portion of the total activity. optimum ph for the a. caviae proteolytic activity is at 7.0. a. caviae produc ... | 1995 | 7473065 |
| changes in microbial populations on fresh cut spinach. | the microbial populations found on fresh-cut spinach leaves that were stored in gas permeable bags at 10 degrees c for 12 days were examined and identified. the microorganisms consisted of mesophilic aerobic bacteria, psychrotrophic bacteria, pseudomonadaceae, enterobacteriaceae, micrococcaceae, lactic acid bacteria and yeasts. populations of mesophiles, psychrotrophs, pseudomonadaceae and enterobacteriaceae increased sharply during the storage period. the initial populations were 10(7), 10(6), ... | 1996 | 8880301 |
| expression of aeromonas caviae bla genes in escherichia coli. | an isolate of aeromonas caviae 035 carried a 55.5 kb self-transferable plasmid. transfer of the plasmid to escherichia coli k12 resulted in the expression of a tem-like beta-lactamase that was not expressed in parental a. caviae. the bla gene sequence was detectable by dna hybridization and pcr amplification of the plasmid when extracted from parental a. caviae or from e. coli k12 transconjugants. sequence analysis of the bla genes revealed in each case an 858 bp open reading frame, differing fr ... | 1996 | 8889718 |
| diarrhea associated with aeromonas species in children in day care centers. | outbreaks of diarrhea caused by enteropathogens have been reported in day care centers (dcc), but aeromonas species have not been implicated. this study evaluated 381 children involved in 51 outbreaks in four dcc to determine the association of aeromonas species with diarrhea and to characterize the isolates. the organism was identified in two outbreaks of diarrhea. in one, aeromonas species were isolated from 6 (24%) of 25 children and in the other from 5 (21%) of 24 children. seven other aerom ... | 1993 | 8515113 |
| characterization of cytotoxic, hemolytic aeromonas caviae clinical isolates and their identification by determining presence of a unique hemolysin gene. | aeromonas caviae has recently been recognized as an important enteropathogen and its hemolysin is purported to be one of the virulence factors, in this study, a total of 80 clinical isolates of aeromonas spp. were investigated by pcr with synthetic oligonucleotides targeting a cloned hemolysin-encoding sequence from an a. caviae isolate of clinical origin. of the 35 clinical a. caviae isolates tested, only 6 contained the target sequence. | 1996 | 8940472 |
| adherence of aeromonas caviae to human cell lines hep-2 and caco-2. | adherence of aeromonas caviae to hep-2 and caco-2 cell monolayers was investigated with 24 clinical isolates. growth phase, temperature, multiplicity of infection and length of incubation affected adherence. treatment of the bacteria with trypsin, sodium metaperiodate, mechanical shearing and the addition of cytochalasin b and cycloheximide to the monolayer significantly reduced the adherence capabilities of the strains investigated. the use of chloramphenicol to inhibit protein synthesis reduce ... | 1996 | 8958248 |
| characterization of aeromonas spp. isolated from humans with diarrhea, from healthy controls, and from surface water in bangladesh. | aeromonas isolates from patients with diarrhea in bangladesh (n = 69), from healthy controls (n = 11), and from surface water (n = 40) were analyzed with respect to their hybridization groups (hgs) by the aid of fatty acid methyl ester (fame) characterization and dna fingerprinting by aflp, biochemical phenotypes (phe-neplate [php] types), and the production of hemolysin and cytotoxin. the aim of the investigation was to find out whether certain strains carrying virulence factors predominated am ... | 1997 | 9003598 |
| rapid and sensitive method for the detection of aeromonas caviae and aeromonas trota by polymerase chain reaction. | a 16s rdna-based polymerase chain reaction (pcr) method was developed for the detection of aeromonas caviae and aeromonas trota. these two species were identified from other aeromonas spp. and closely related species by primers set (aer1 and aer2). the amplified product was 316 bp. the identity of the amplified product was confirmed by dna-dna hybridization. two sets of primers (aer8 and aer9) were used for specific identification of aer. caviae. amplifying the 260 bp fragment of 16s rrna gene r ... | 1997 | 9134769 |
| the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis. | from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ... | 1996 | 9147724 |
| adherence and invasion of aeromonas caviae to monolayer cells. adherence and invasion of aeromonas caviae. | | 1997 | 9192016 |
| evaluation of the synergistic haemolysis (camp-like) test in the identification of motile, mesophilic aeromonas species. | usefulness of the synergistic haemolysis (camp-like) test in the identification of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae was evaluated, using beta-toxin producing staphylococcus aureus atcc 25923 and alpha-toxin producing clostridium perfringens atcc 13124 as sphingomyelinase producing indicator strains. the tests were done on columbia blood agar base supplemented with 5% sheep blood (cba; difco laboratories, detroit, mi, usa). when cultured aerobically, 77% of a. hydrophi ... | 1997 | 9241799 |
| cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of aeromonas caviae. | a 5.0-kbp ecorv-ecori restriction fragment was cloned and analyzed from genomic dna of aeromonas caviae, a bacterium producing a copolyester of (r)-3-hydroxybutyrate (3hb) and (r)-3-hydroxyhexanoate (3hhx) [p(3hb-co-3hhx)] from alkanoic acids or oils. the nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (pha) synthase gene (phac(ac) [i.e., the phac gene from a. caviae]) together with four open reading frames (orf1, -3, -4, and -5) and one putative promoter region. t ... | 1997 | 9244271 |
| distribution and expression of beta-lactamase genes among aeromonas spp. | clinical and environmental isolates of aeromonas spp. (20 aeromonas caviae, 33 aeromonas veronii and 21 aeromonas hydrophila) were examined for their ability to yield mutants derepressed for beta-lactamase production and for the distribution of the three chromosomally encoded beta-lactamase genes, amps and ceps from a. veronii bv. sobria and cpha from a. hydrophila. of these isolates, 100% and 96% of the isolates from a. hydrophila and a. caviae, respectively, yielded beta-lactamase derepressed ... | 1997 | 9301981 |