| effects of histamine-sensitizing factor and cortisol on lymphocyte adenyl cyclase responses. | levels of cyclic adenosime monophosphate (camp) in lymphocytes are regulated by beta-adrenergic agonists and pge1. the effect of these agonists is potentiated by cortisol. incubation of lymphocytes with histamine-sensitizing factor (hsf) leads to loss of response to epinephrine and pge1. the response can be partially restored by cortisol. incubation of lymphocytes with beta-adrenergic antagonists such as propranolol leads to the loss of beta-adrenergic agents but not pge1. the inhibition by prop ... | 1977 | 833377 |
| ir-gene control of antibody class production. | the antibody response in mice to dnp-insulin is under ir-gene control. the ir gene defects in two strains have been analyzed. in both cases the igg immune response was impaired whereas the igm response was not affected. one h-2 gene haplotype was characterized by lack of igg response, independent of the immunization protocol. a second h-2 haplotype manifested a low response of igg after immunization with bordetella pertussis as an adjuvant but a high response after complete freund's adjuvant. it ... | 1977 | 839064 |
| passive cutaneous anaphylaxis and vasoactive amine challenge in nude (nu/nu) mice. a comparison with nu/+, axenic and young normal animals. | recent suggestions of a thymic origin or thymo-dependent differentiation of tissue mast cells prompted an investigation in the athymic nude (nu/nu) mouse. the outbred nu/nu examined were found to possess mast cells in at least comparable numbers to the phenotypically normal nu/+. these nu/nu were superior to nu/+ as recipient for mast cell-dependent, long latent period (ige-type), passive cutaneous anaphylactic (pca) reactions. a variety of studies performed, including competition with nu/+ seru ... | 1977 | 844890 |
| erythromycin prophylaxis for pertussis. | a neonate admitted to the newborn nursery was found to have bacteriologically proven bordetella pertussis whooping cough, and two of seven infants exposed to this child became infected. erythromycin administration to the seven infants apparently prevented further infection as well as the appearance of clinical disease in the two infected infants. this limited experience supports previous reports of the efficacy of erythromycin in preventing b. pertussis infection and disease in susceptible child ... | 1977 | 850603 |
| prevention of homocytotropic antibody formation and anaphylactic sensitization by prefeeding antigen. | studies were conducted in rats to determine whether pre-feeding antigen can prevent the development of bordetella pertussis-induced homocytotropic antibody (hca) formation and anaphylactic sensitization to the fed antigen. da rats fed ragweed for a minimum of 2 wk and sprague-dawley rats fed horse serum for at least 4 wk demonstrated specific unresponsiveness to anaphylactic sensitization as measured by their inability to form hca and their normotensive response to intravenous challenge with ant ... | 1977 | 893876 |
| evidence for suppressor cells in lewis rats' experimental allergic encephalomyelitis. | in this work we demonstrate a suppressive activity on the induction of experimental allergic encephalomyelitis (eae) in lewis rats, transferable to syngeneic animals, challenged with encephalitogenic mixture (myelin basic protein, complete freud's adjuvant plus bordetella pertussis organisms) 24 h later. this activity is probably effected by t cells and not by (an) inhibitory serum factor(s). the induction of this specific protection could be due to the penetration of the myelin basic protein an ... | 1977 | 923633 |
| adjuvant effect of bordetella pertussis vaccine to sheep erythrocytes in mice: enhancement of cell-mediated immunity by subcutaneous administration of adjuvant and antigen. | the subcutaneous route (s.c.) was used to study the adjuvant effect of bordetella pertussis vaccine (pv) on cell-mediated immunity to sheep erythorcytes (srbc). the immune response was measured by a sensitive assay procedure in which the antigen is injected intracutaneously into the mouse ear and the inflammatory swelling is measured with calipers. pv significantly enhanced cell-mediated immunity to srbc, and the enhancement persisted for at least 3 weeks. pv administered up to 6 days before srb ... | 1977 | 924677 |
| host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines. i. description and characterization of adoptive transfer by immune spleen cells. | cellular immune responses after immunization with a number of inactivated venezuelan equine encephalomyelitis (vee) virus vaccines were evaluated in mice by means of an adoptive transfer system. formalin-inactivated, tc-83 strain vee virus vaccine was immunogenic and highly effective in protecting recipient mice against challenge with virulent vee virus. in contrast to immunization with live tc-83 vee virus vaccine, however, immunization with inactivated vee vaccine did not provide donor mice wi ... | 1976 | 939921 |
| induction and suppression of reagins in the neonatal mouse. | neonatal swr mice are capable of synthesizing reagins when immunized with a mixture of concanavalin and ovalbumin or a mixture of bordetella pertussis, alum and ovalbumin. reaginic antibody-forming cells can be found in the spleen, lymph nodes, bone marrow and peyer's patches. tolerance with respect to ige can be induced by the injection of deaggregated ovalbumin into neonatal mice. | 1976 | 945227 |
| activation and consumption of hageman factor in the anaphylactic shock of the rat. | in the early stages of anaphylactic shock of rats pretreated with bordetella pertussis vaccine, a prompt and parallel activation of the factor xiia-dependent intrinsic coagulation, kinin generation, and fibrinolytic acticity was observed. the coagulation studies, the similarity of anaphylactic results with those produced by a single injection of ellagic acid, and the effective inhibition of the anaphylactic and the ellagic acid-induced activation of these pathways by lysozyme all suggest that fa ... | 1976 | 965106 |
| pertussis vaccine pleurisy: a model of delayed hypersensitivity. | male wistar rats have been sensitized to bordetella pertussis using a mixture of freund's incomplete adjuvant and pertussis organisms. intrapleural challenge 12 days later with pertussis produced a marked delayed inflammatory response, maximal at 48 hours and dominated by influx of mononuclear cells. dosing with d-penicillamine (25 mg/kg) and levamisole (5 mg/kg) at the time of challenge produced a significant enhancement of the reaction. a long period of dosing with either drug, or treatment wi ... | 1976 | 970291 |
| macrophage function and host resistance against infection with toxoplasma gondii. | the role of macrophages on the course of an infection with toxoplasma gondii has been examined. stimulation of macrophage function by killed bordetella pertussis cells did not show any beneficial effect as an increased susceptibility became apparent. the functional blockade of macrophages by dextran sulfate or carbon particles did not result in a higher susceptibility of mice to the lethal primary infection with t. gondii. thus in vivo macrophages apparently do not play an essential role as effe ... | 1976 | 974901 |
| kinetics and localization of ige tetanus antibody response in mice immunized by the intratracheal, intraperitoneal and subcutaneous routes. | the heterologous adoptive cutaneous anaphylaxis system was used to determine the kinetics of appearance of ige-producing cells in various lymphoid tissues of mice following intratracheal (i.t.), intraperitoneal (i.p.), or subcutaneous (s.c.) immunization with tetanus toxoid and bordetella pertussis organisms. immunization, i.t. and i.p., produced similar patterns of response with the bronchial lymph nodes quantitatively exceeding the responses in other lymphoid tissues. in both cases the splenic ... | 1976 | 992717 |
| the effect of formaldehyde on the toxicity and potency of pertussis vaccine. | storage of concentrated suspensions of bordetella pertussis organisms at 5 c for long periods of time in the presence of 0.02% thimerosal as well as heating at 56 c for 30 minutes cannot be relied upon to detoxify the vaccines. our data show that the toxic thimerosal-killed vaccines can be rapidly detoxified by adding formalin to a final concentration of 0.025-0.05% and incubating at temperatures of 5-20 c for from one to three months. these formalin-detoxified vaccines are protective and meet p ... | 1975 | 1053237 |
| stimulation of lymphocytes of patients administered with a trypsin inhibitor, trasylol (basic pancreatic trypsin inhibitor pharmaceutical), in vitro with bpti and other several stimulants. | distinguished lymphocytes stimulation was observed in vitro with one out of four patients received trasylol therapy. the peak response of dna synthesis was shown to occur at 100 mug/tube of basic pancreatic trypsin inhibitor (bpti) itself, about 6 days after addition of bpti. reactivity (3h-thymidine incorporation) of lymphocytes of patients after the therapy, to specific antigen, ie., purified protein derivative (ppd) or bordetella pertussis organisms in vitro, was markedly depressed. on the ot ... | 1975 | 1085380 |
| quantitation of circulating t and b lymphocytes in children with whooping cough. | the numbers of circulating t and b lymphocytes in seven children with whooping cough due to bordetella pertussis and eight control subjects were determined. all the children with whooping cough had an absolute lymphocytosis (mean 29,142/mm3vs. 5,225/mm3) and by surface marker criteria both t cells and b cells were increased (mean t cells, whooping cough 15,794/mm3 vs. 3,516/mm3 controls; mean b cells, 13,393/mm3 whooping cough vs. 1,706/mm3 controls). however, the ratios of t cells to b cells in ... | 1976 | 1087001 |
| studies on immune responses to parasite antigens in mice. ii. aspects of the t cell dependence of circulating reagin production to ascaris suum antigens. | heat-labile, rat skin-fixing antibodies were detected readily in the sera of young female mice dosed intranasally with the body fluid of ascaris suum (abf) and the adjuvant, bordetella pertussis vaccine (bpv). in addition, washed cell suspensions prepared from spleen and the lymph nodes regional to the lungs were positive in an adoptive cutaneous anaphylaxis assay, an assay which may detect activities of reagins associated with mast cells rather than reaginic antibody-secreting cells. the intrap ... | 1976 | 1087924 |
| adjuvant effect of bordetella pertussis vaccine to sheep erythrocytes: enhancement of antibody formation by using subcutaneous administration of adjuvant and antigen. | the subcutaneous route (s.c.) was used to study the adjuvant effect of bordetella pertussis vaccine (pv) on the primary antibody response to sheep erythrocytes. the reasons for using the s.c. route are discussed. pv, besides enhancing the hemagglutinin response, also markedly increased the number of plaque-forming cells in the draining lymph nodes. a heated preparation of pv was tested and found to possess significant adjuvant activity. interestingly, the enhancement occurred in the absence of m ... | 1975 | 1104487 |
| evidence of fibrinogen degradation in rat anaphylaxis. | in active anaphylactic shock of rats pretreated with bordetella pertussis vaccine, both plasma thrombin clotting time and the amount of antigenically active fibrinogen degradation products in the serum were increased. the formation of clottable fibrinogen fragments was shown by sds polyacrylamide gel electrophoresis of thrombin-induced clots. when plasma of rats pretreated with 125i rat fibrinogen and then subjected to anaphylaxis was submitted to sds polyacrylamide gel electrophoresis, fibrinog ... | 1975 | 1158526 |
| [leukocytes and antibody activity in lung infection by bordetella pertussis in mice]. | | 1975 | 1240236 |
| mechanism of reappearance of contact sensitivity to dncb in desensitized guinea pigs. | the different immunologic activities of lymph node and spleen cells as compared to peritoneal exudate cells were studied in temporarily desensitized, dncb-contact-sensitive guinea pigs. lymph node (and spleen) lymphocytes: (a) transfer contact sensitivity to syngeneic recipients when harvested at the time of unresponsiveness; (b) are only temporarily inactivated in tolerant recipients by the desensitizing procedure; (c) enter the lymph node (or spleen) compartments in a higher proportion than pe ... | 1976 | 1245756 |
| sensitizing capacity of syngeneic, allogeneic and xenogeneic ocular tissue. effect on intact and injured rat eyes. | experimental allergic uveoretinitis could not be induced by the following procedures in a total of 87 rats of four different inbred strains; intramuscular or intraperitoneal immunization with syngeneic, allogeneic or xenogeneic native ocular tissue (up to 80 eyes, treatment up to 15 months); intramuscular and subcutaneous application of complete freund's adjuvants and bordetella pertussis vaccine followed by perforation (induction of uveal and lenticular lesions) of the left eye. | 1976 | 1250241 |
| acute effects of bordetella pertussis vaccine in vitro. | | 1976 | 1263184 |
| absolute configuration of 3-hydroxy fatty acids present in lipopolysaccharides from various bacterial groups. | the absolute configuration of 3-hydroxy fatty acids has been studied, which are present in the lipopolysaccharides of the following bacteria: phodopseudomonas gelatinosa, rh. viridis, rhodospirillum tenue, chromobacterium violaceum, pseudomonas aeruginosa, bordetella pertussis, vibrio metchnikovii, vibrio cholerae, salmonella spp., escherichia coli, shigella flexneri, proteus mirabilis, yersinia enterocolitica and fusobacterium nucleatum. the 3-hydroxy acids were liberated by strong alkaline hyd ... | 1976 | 1278168 |
| the epidemiology of pertussis in england and wales. | recovery in the uptake of whole-cell pertussis vaccine from 30% in 1978 to 91% in 1992 has resulted in a major reduction in the incidence of pertussis and in the proportion of cases occurring in pre-school children. this has been accompanied by a decline in pertussis mortality rates, with no deaths reported since september 1990. seventy-four per cent of the 50 fatal cases reported since 1980 have been infants under one year of age, demonstrating the importance of completing primary immunisation ... | 1992 | 1285134 |
| immunosuppressive effects induced by the polysaccharide moiety of some bacterial lipopolysaccharides. | the immunomodulatory properties of several lipopolysaccharides (lps) derived from clinical isolates of pseudomonas aeruginosa, branhamella catarrhalis, and bordetella pertussis were evaluated for their capacity to influence the magnitude of the antibody response to type iii pneumococcal polysaccharide (sss-iii), which is known to be regulated by suppressor and amplifier t cells (ts and ta, respectively). the administration of lps, two days after immunization resulted in a significant increase in ... | 1992 | 1286878 |
| epidemiology of pertussis and reactions to pertussis vaccine. | it remains clear that pertussis is a dangerous infectious disease that is well-controlled in industrialized countries by widespread immunization. in the developing world, it remains a source of high morbidity and mortality because of previously inadequate immunization programs. however, because of the intense efforts of the world health organization's expanded programme on immunization, the effects of pertussis have already been ameliorated and show promise of being within a decade of approximat ... | 1992 | 1289114 |
| bordetella pertussis extract induces increase in the activities of glycolytic enzymes in mouse liver. | the hypoglycemic effect of bordetella pertussis (challenge strain no.18323) purified cell extract (protein with traces of carbohydrates, 2 mg%) administered (0.1 mg/100 g body wt. i.v.) into mice on the activities of the key regulatory enzymes, viz. glucokinase, phosphofructokinase, pyruvate kinase, glyceraldehyde phosphodehydrogenase, glucose-6-phosphate dehydrogenase (g-6-pd) and lactate dehydrogenase, of glycolytic pathway in liver has been studied at varying intervals after injection. the ma ... | 1992 | 1289237 |
| cloning and sequence analysis of the evgas genes involved in signal transduction of escherichia coli k-12. | we have cloned and sequenced new escherichia coli genes which belong to member of the family of environmentally responsive two-component system and named evga and evgs because their amino acid sequences were found the most homologus to the bordetella pertussis bvga and bvgs. they were mapped at 51 min. and extending from 6b9 to 7g9 in the kohara miniset library of the e. coli chromosome. in fact, both evga and evgs proteins predicted from their dna sequences were identified in the in vitro coupl ... | 1992 | 1289796 |
| laboratory correlates of protection and protective immunity to bordetella pertussis. | | 1992 | 1295347 |
| effects of ketotifen on airway responses to allergen challenge in the actively sensitized brown norway rat. | the purpose of the study was to examine the effect of ketotifen on the airway responses and the recruitment of the inflammatory cells into the airways of sensitized rats after antigen challenge. twenty-five brown norway rats, 7-9 weeks old, were actively sensitized to ovalbumin (oa) (1 mg s.c.) and bordetella pertussis vaccine (10(9) bacilli i.p.). at 14 days after sensitization rats were anesthetized with urethane (1.1 g/kg i.p.) and intubated endotracheally. aerosols of oa (5% w/v in saline fo ... | 1992 | 1295373 |
| bordetella pertussis fha antibodies in maternal/infants sera and colostrum. | the high incidence of pertussis in the first year of life confirms that susceptibility remains high for children in this age group despite > 90% pertussis vaccine compliance. in this respect, immunoresponse to bordetella pertussis was investigated. filamentous hemagglutinin (fha) antibodies were studied due to their important protective role, in blocking the adherence of the bacteria to respiratory tract ciliated cells. the relative rate of detection and degree of positivity of igg and iga antib ... | 1992 | 1295946 |
| siderophore production and membrane alterations by bordetella pertussis in response to iron starvation. | bordetella pertussis was grown in iron (fe)-free defined medium to limit the growth of the organism. doubling times of the fe-starved organism increased by approximately 1 h, and a 40% reduction in the final extent of growth in fe-depleted medium was observed. under these conditions, a hydroxamate siderophore named bordetellin was secreted by b. pertussis. lactoferrin and transferrin supported growth of b. pertussis even when the protein was sequestered inside dialysis tubing. this suggested tha ... | 1992 | 1309510 |
| signal transduction during antibody-dependent cellular cytotoxicity mediated by u937 cells. | stimulation of the human promonocytic cell line u937 with antibody-coated chicken red blood cells (ab-crbc), leads to inositol phosphate (ip) release in the effector cells. neomycin (5 x 10(-4) m) completely inhibits activation of phosphoinositide breakdown, while adcc is suppressed in a dose-dependent manner. bordetella pertussis toxin (pt) (0.5 micrograms/ml), entirely inhibits ip release, while adcc activity is markedly suppressed. the pkc inhibitors h-7 and propranolol also suppress adcc. ha ... | 1992 | 1312511 |
| bordetella pertussis induces respiratory burst activity in human polymorphonuclear leukocytes. | virulent bordetella pertussis strains survive intracellularly within human polymorphonuclear leukocytes (pmn), at least in part because of inhibition of phagosome-lysosome fusion (l. l. steed, m. setareh, and r. l. friedman, j. leukocyte biol. 50:321-330, 1991). further investigations were done to determine if b. pertussis also inhibited respiratory burst activity of pmn as an additional mechanism of intracellular survival. chemiluminescence and flow cytometry assays showed that b. pertussis ind ... | 1992 | 1314225 |
| adenosine 3',5'-monophosphate suppresses metastatic spread in nude mice of steroidogenic rat granulosa cells transformed by simian virus-40 and ha-ras oncogene. | 8-bromo-camp and substances elevating camp levels within cells, such as forskolin, cholera toxin, and bordetella pertussis-invasive adenylate cyclase (bpac), suppress the growth of cultured granulosa cells cotransfected by simian virus-40 (sv40) dna and ha-ras oncogene concomitantly with the induction of steroidogenesis and without affecting oncogene expression. we, therefore, tested the hypothesis that camp can modulate tumorigenesis and metastatic spread of these cells in vivo. the cotransfect ... | 1992 | 1319328 |
| enzymatic activity of adenylate cyclase toxin from bordetella pertussis is not required for hemolysis. | adenylate cyclase (ac) toxin from bordetella pertussis enters cells to cause supraphysiologic increases in camp. ac toxin is also hemolytic. substitution of lys-58 with a methionine residue by site-directed mutagenesis of the structural gene for ac toxin, cyaa, and introduction of this mutation onto the b. pertussis chromosome results in an organism that synthesizes an enzyme-deficient ac toxin molecule. this mutant toxin molecule exhibits 1000-fold reduction in enzymatic activity relative to wi ... | 1992 | 1319923 |
| isolation and molecular characterization of a novel broad-host-range plasmid from bordetella bronchiseptica with sequence similarities to plasmids from gram-positive organisms. | a 2.6 kb plasmid, named pbbr1, was isolated from bordetella bronchiseptica s87. after insertion of an antibiotic resistance marker, this plasmid could be transferred into escherichia coli, bordetella pertussis, b. bronchiseptica, vibrio cholerae, rhizobium meliloti, and pseudomonas putida by transformation or conjugation. conjugation was possible only when the incp group transfer functions were provided in trans. as shown by incompatibility testing, pbbr1 does not belong to the broad-host-range ... | 1992 | 1321324 |
| trans-acpd inhibits camp formation via a pertussis toxin-sensitive g-protein. | in primary cultured striatal neurons we found that (+-)-trans-1-amino-cyclopentyl-1,3-dicarboxylate (trans-acpd) could inhibit forskolin-induced camp formation in a dose-dependent manner (ec50 156 +/- 38 microm, n = 5, maximal inhibition 37.8 +/- 1.2, n = 37). the trans-acpd-induced inhibition was totally abolished in neurons preincubated with bordetella pertussis toxin (1 microgram/ml), demonstrating the involvement of a g-protein. this is the first report in intact neurons of a glutamate metab ... | 1992 | 1323480 |
| preparative separation of foreign antigens for highly efficient presentation to t cells in vitro. | a method is described for the separation and purification of proteins from complex mixtures of foreign antigens in a form suitable for stimulating t cells in vitro. the technique involves electrophoretic separation of proteins followed by elution, concentration and adsorption of the polypeptide subunits to latex microspheres. alternatively, where a specific antibody is available, proteins may be affinity-purified from a heterogeneous mixture of antigens, using antibody-coated latex microspheres. ... | 1992 | 1335464 |
| construction and analysis of bordetella pertussis mutants defective in the production of fimbriae. | although the role of fimbriae in bacterial disease has been well established, little is known about the function of bordetella pertussis fimbriae. to study this function, well-defined fimbrial mutants were constructed. b. pertussis harbours three fimbrial genes, fim2, fim3 and fimx, and strains were constructed in which one or more fimbrial genes were inactivated by means of gene replacement. analysis of these strains by means of immunoblotting suggested the presence of a fourth fimbrial gene, t ... | 1992 | 1350044 |
| characterization of a metabotropic glutamate receptor: direct negative coupling to adenylyl cyclase and involvement of a pertussis toxin-sensitive g protein. | we have characterized a g-protein-coupled glutamate receptor in primary cultures of striatal neurons. glutamate, quisqualate, or trans-1-aminocyclopentane-1,3-dicarboxylate inhibited by 30-40% either forskolin-stimulated camp production in intact cells or forskolin plus vasoactive intestinal peptide-activated adenylyl cyclase assayed in neuronal membrane preparations. these inhibitory effects were suppressed after treatment of striatal neurons with bordetella pertussis toxin, suggesting the invo ... | 1992 | 1355603 |
| gene replacement in bordetella pertussis by transformation with linear dna. | we replaced the wild-type tox operon of bordetella pertussis with in vitro mutated, detoxified alleles by electroporetic transformation using unmarked linear dna. uptake of dna was selected by transient ampicillin resistance and two simultaneous recombination events resulted in gene-replacement at the natural locus with no integration of heterologous dna. tox alleles were stable without selection and recombinant strains secreted non-toxic, fully assembled, protective pertussis toxin (pt) analogu ... | 1990 | 1366929 |
| protective surface antigen p69 of bordetella pertussis: its characterization and very high level expression in escherichia coli. | the surface antigen, p69 of bordetella pertussis, an n-terminal fragment of the precursor protein, p93, is likely to be an important component of future subunit vaccines against whooping cough. we have expressed several defined n-terminal fragments of p93 in e. coli and compared their electrophoretic mobilities with that of purified p69 from b. pertussis. these experiments show that p69 is considerably smaller than the 69 kd originally estimated from its gel mobility and is probably 60.4 kd in s ... | 1990 | 1366930 |
| the effect of ph on the production of pertussis toxin by bordetella pertussis. | the production of pertussis toxin by bordetella pertussis was increased by controlling the ph at 7.0 through the addition of sulfuric acid. the more commonly used hydrochloric acid and tris buffer were observed to be detrimental to toxin yields. | 1991 | 1366987 |
| production of cell mass and pertussis toxin by bordetella pertussis. | the cultivation of bordetella pertussis affects production of pertussis toxin and biomass. comparison of batch mode, chemostat operation and phstat-turbidostatic control showed that productivities for the continuous process were greater than that for the batch operation. continuous operation in balanced growth at the maximum specific growth rate, provided by the phstat, resulted in the maximum specific production rate. because of the strong association of pertussis toxin synthesis and cell growt ... | 1991 | 1367364 |
| high frequency of independent is50 transposition during tn5 mutagenesis of bordetella pertussis virulence-associated genes. | transposon tn5-generated mutants of bordetella pertussis were selected on the basis of their inability to bind the dye congo red (crb-). no mutants which were solely crb- were found. ten mutants were phenotypically equivalent to previously described strains with mutations in the virulence regulatory (bvg) locus and failed to express a range of virulence-associated factors. two of these mutants were shown to have tn5 insertions within the bvg locus, while another two mutants showed deletions in t ... | 1992 | 1368786 |
| immunological characterization of the lipooligosaccharide b band of bordetella pertussis. | two structurally and immunologically different components of bordetella pertussis endotoxin can be visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining: a major a band and a faster-migrating minor b band. certain mutant strains of b. pertussis express only the b band, while the wild-type strains produce both lipooligosaccharides (los). two monoclonal antibodies (mabs) directed against the minor los b band were generated, allowing the study of this surface m ... | 1992 | 1377181 |
| recombinant acellular pertussis vaccine--from the laboratory to the clinic: improving the quality of the immune response. | vaccination is the most effective way to prevent infectious diseases. recombinant dna technologies have provided powerful new tools to develop vaccines that were previously impossible or difficult to make, and to improve the vaccines that were already available but had been developed using old technology. in the case of whooping cough, an effective vaccine (composed of killed bacterial cells) is available, but its use is controversial because of the many side effects that have been associated wi ... | 1992 | 1384602 |
| removal of endotoxin from culture supernatant of bordetella pertussis with aminated poly(gamma-methyl l-glutamate) spherical beads. | attempts were made to prepare adsorbents having a high affinity for endotoxin in the culture supernatant of bordetella pertussis. when poly(gamma-methyl l-glutamate) (pmlg) was used as a matrix and amino groups as the ligand, the highest affinity for endotoxin was attained even at a high ionic strength (mu = 0.2-0.4). pmlg beads containing amino groups of about 3.2 meq/g selectively removed endotoxin from the culture supernatant of b. pertussis without affecting the protective antigens. it was d ... | 1992 | 1384997 |
| is bordetella pertussis clonal? | to establish whether bordetella pertussis is essentially clonal. | 1992 | 1392709 |
| is bordetella pertussis clonal? | | 1992 | 1392809 |
| characterization of a salmonella typhimurium aro vaccine strain expressing the p.69 antigen of bordetella pertussis. | the p.69 bordetella pertussis protective antigen was expressed by use of the trc promoter from the chromosome of a salmonella typhimurium aro vaccine strain, brd509, by integrating the prn gene, encoding the 93-kda precursor of this protein, into the aroc locus. p.69 was detected on the cell surface of the s. typhimurium strain (brd640) by agglutination and immunoelectron microscopy. balb/c mice immunized orally or intravenously with brd640 showed a significant level of protection against an aer ... | 1992 | 1398911 |
| specific lung mucosal and systemic immune responses after oral immunization of mice with salmonella typhimurium aroa, salmonella typhi ty21a, and invasive escherichia coli expressing recombinant pertussis toxin s1 subunit. | pertussis toxin (pt) is considered an essential protective component for incorporation into new generation vaccines against bordetella pertussis, the causative agent of whooping cough. traditionally, antipertussis vaccination has employed an intramuscular route. an alternative to this approach is to stimulate mucosal and systemic immune responses by oral immunization with live vaccine carrier strains of salmonella spp. or escherichia coli. recombinant s1 subunit of pertussis toxin was expressed ... | 1992 | 1398937 |
| proliferative responses and gamma interferon and tumor necrosis factor production by lymphocytes isolated from tracheobroncheal lymph nodes and spleen of mice aerosol infected with bordetella pertussis. | a group of mice was aerosol infected with live, virulent bordetella pertussis bacteria. during a period of 7 weeks following the infection, with intervals of 1 week, lymphocytes were isolated from the tracheobroncheal lymph nodes (tbl) and the spleens (spl) of the infected mice. the in vitro proliferative responses as well as the gamma interferon and tumor necrosis factor production levels of the isolated lymphocytes in response to stimulation with whole killed b. pertussis bacteria were measure ... | 1992 | 1398968 |
| uptake and intracellular survival of bordetella pertussis in human macrophages. | recent reports have demonstrated that bordetella pertussis has invasive behavior in vivo and in vitro. in this study, we investigated the ability of a virulent strain, avirulent mutants, and mutants deficient in specific virulence factors to enter and survive intracellularly in human macrophages in vitro. uptake of virulent b. pertussis was dose dependent and occurred in the absence of serum or specific antibody, with entry occurring via a microfilament-dependent phagocytic process. the virulent ... | 1992 | 1398970 |
| recombinant interleukin-8 induces changes in cytosolic ca2+ in human neutrophils. | activation of polymorphonuclear leukocytes (pmnl) by most soluble stimulants is associated with a marked increase in cytosolic free ca2+ ([ca2+]i). interleukin-8 (il-8), a monocyte-derived neutrophil chemotactic factor and potent neutrophil-activating cytokine, effectively enhanced the resting free [ca2+]i within human pmnl in a dose-dependent manner (maximal effect with 100 ng/ml). the increase in [ca2+]i was substantially (55%) inhibited in the absence of extracellular ca2+. thus, the increase ... | 1992 | 1402020 |
| [protective activity of tea and catechins against bordetella pertussis]. | we examined the bactericidal activity of tea and catechins against bordetella pertussis. green tea, black tea and coffee showed marked bactericidal activity at their concentrations in beverages, while pu-erh tea killed the bacteria in a moderate way. (-) epigallocatechin gallate (egcg) and theaflavin digallate (tf3) showed also marked bactericidal activity. green tea and black tea also effectively blocked the adhesion of b. pertussis to hela and cho cells, whereas ecgg and tf3 could not. egcg an ... | 1992 | 1402092 |
| antibody response to bordetella pertussis antigens after immunization with american and canadian whole-cell vaccines. | because of apparent differences in the incidence and epidemiology of pertussis in the united states and canada, we measured the antibody response to four bordetella pertussis antigens and to a whole-bacteria preparation in children immunized with american and canadian whole-cell pertussis vaccines. all infants received combined pertussis, tetanus, and diphtheria vaccines from one of two american manufacturers or a single canadian manufacturer. the canadian children received either oral poliomyel ... | 1992 | 1403383 |
| brm activities of low-toxic bordetella pertussis lipopolysaccharides. | a low-toxic lipopolysaccharide (bp-lps) was isolated from killed bordetella pertussis (tohama strain). ld50 of bp-lps was about 0.8 mg/mouse which was about 10-fold higher than the ld50 of e. coli-lps(80 micrograms/mouse). toxicity measured by decrease in body weight of bp-lps-injected mice was similarly low. bp-lps had strong antitumor activities against various murine syngeneic tumors, and its systemic administration caused clear regression of such as mm46 mammary carcinoma and meth a fibrosar ... | 1992 | 1414607 |
| evaluation of an improved dna probe for diagnosis of pertussis. | a bordetella pertussis specific subclone, prz61, of a bordetella genus-specific clone, pb23, was evaluated on nasopharyngeal aspirates of 179 patients with suspected pertussis. hybridization was performed directly after spotting or after 1-3 days of preculture of the nylon membranes on solid culture medium. a direct comparison of the two probes was obtained by reprobing with the subclone the same membranes that had been hybridized with the parent probe. prz61 detected 50% of the serologically de ... | 1992 | 1424512 |
| distinct steps in the penetration of adenylate cyclase toxin of bordetella pertussis into sheep erythrocytes. translocation of the toxin across the membrane. | adenylate cyclase (ac) toxin from bordetella pertussis penetrates eukaryotic cells and upon activation by calmodulin generates unregulated levels of intracellular camp. the process of toxin penetration into sheep erythrocytes was resolved into three consecutive steps including insertion, translocation, and intracellular cleavage. insertion of the toxin into the cell membrane occurred over a wide temperature range (4-36 degrees c). in contrast, translocation of the toxin, i.e. transfer of the nh2 ... | 1992 | 1429610 |
| [an epidemiological study on bordetella pertussis infection in fukui prefecture from 1986 to 1991--especially observation on the patients of culture-confirmed pertussis]. | in order to clarify the epidemiological situation of whooping cough in fukui prefecture, 478 nasopharyngeal swabs from patients with pertussis-like symptoms were submitted to bacterial isolation. laboratory data of these patients with clinical informations at the departments of pediatrics of 6 hospitals in fukui city from june 1986 to may 1991 were also examined in relation to the above bacterial isolation. the results observed in culture positive patients were as follows: 1) b. pertussis were i ... | 1992 | 1431384 |
| cloning and sequencing of a gene encoding a 21-kilodalton outer membrane protein from bordetella avium and expression of the gene in salmonella typhimurium. | three gene libraries of bordetella avium 197 dna were prepared in escherichia coli le392 by using the cosmid vectors pcp13 and pya2329, a derivative of pcp13 specifying spectinomycin resistance. the cosmid libraries were screened with convalescent-phase anti-b. avium turkey sera and polyclonal rabbit antisera against b. avium 197 outer membrane proteins. one e. coli recombinant clone produced a 56-kda protein which reacted with convalescent-phase serum from a turkey infected with b. avium 197. i ... | 1992 | 1447140 |
| bordetella pertussis versus non-l. pneumophila legionella spp.: a continuing diagnostic challenge. | | 1992 | 1452724 |
| bacteriological and serological study of pertussis in abeokuta, nigeria. | charcoal horse blood agar is the medium of choice for isolation of bordetella pertussis from patients with early whooping cough. since sterile animal blood often is not available in developing countries, a field study in nigeria was undertaken to evaluate donated human blood as supplement to charcoal agar. out of 209 children with suspected early pertussis, 33 were culture-positive (isolation rate 16%). out of 188 children studied serologically by enzyme immunoassay, 36 (19%) were seropositive. ... | 1992 | 1455525 |
| guanine nucleotide binding proteins in the dual regulation of lacrimal function. | the purpose of this study was to identify and characterize functional g proteins that couple regulatory peptides with lacrimal secretory functions. membranes were prepared from isolated rat exorbital lacrimal gland acini, and guanosine 5'-triphosphate (gtp)-dependence of adenylyl cyclase activity, known to be coupled with regulation of secretion, was measured. the guanine nucleotide gtp produced a biphasic response in the activity of membrane-bound adenylyl cyclase during a 10 min incubation wit ... | 1992 | 1464505 |
| [the evaluation of the mutagenic activity of pertussis vaccinal preparations]. | two vaccine preparations obtained from bordetella pertussis, whole-cell vaccine constituting one of the components of adsorbed dpt vaccine and acellular vaccine, were tested for mutagenicity. the doses of the preparations covered the range 1-100 ed50. ames' test and the metaphase analysis of marrow cells of c57bl/6j mice were used. the acellular preparation was also tested on thymectomized mice, taking into consideration chromosomal aberrations in marrow metaphases. whole-cell and acellular pert ... | 1992 | 1466172 |
| prolonged survival of bordetella pertussis in a simple buffer after nasopharyngeal secretion aspiration. | a simple method for recovery of bordetella pertussis is described using phosphate-buffered saline containing a casein hydrolysate for transporting secretions collected by nasopharyngeal aspirate. bordetella pertussis was reisolated from 92% of clinical specimens held at 4 degrees c for 1 week and from all specimens held at -20 degrees c. this method will facilitate the centralization of laboratory facilities for the diagnosis of pertussis. | 1992 | 1477793 |
| serratia marcescens forms a new type of cytolysin. | most serratia marcescens strains produce a new type of cytolysin (hemolysin) which is also found in other serratia species. the hemolytic polypeptide shla (m(r) 162 101) is secreted across the outer membrane through the help of the shlb protein which also involves conversion of an inactive precursor in an hemolytically active form. both proteins are synthesized with signal sequences which are released during export across the cytoplasmic membrane. mutants expressing inactive shlb derivatives are ... | 1992 | 1478465 |
| [basic and clinical study of meropenem in pediatric field]. | meropenem (mepm), a novel parenteral carbapenem antibiotic, was examined in a cooperative study involving 12 pediatric and 1 neonatologic facilities. the results are summarized as follows. 1. antibacterial activity antibacterial activity of mepm against stock organisms including 31 strains of streptococcus agalactiae, 14 of listeria monocytogenes, 4 of bordetella pertussis and 3 of neisseria meningitidis ranged from 0.025 to 0.10 micrograms/ml in mic90's, which were equal or lower than those of ... | 1992 | 1479687 |
| bacterial agents protect against autoimmune disease. i. mice pre-exposed to bordetella pertussis or mycobacterium tuberculosis are highly refractory to induction of experimental autoimmune encephalomyelitis. | infectious agents have often been implicated in the etiology of autoimmune diseases. here we show that bacteria may also play a role in resistance to autoimmune diseases. sjl/j and (sjl/j x balb/c)f1 mice are genetically susceptible to induction of experimental autoimmune encephalomyelitis (eae), a murine model for human demyelinating autoimmune diseases such as multiple sclerosis. we studied the effect of several bacteria on the development of eae and found that exposure of sjl/j or (sjl/j x ba ... | 1992 | 1489483 |
| inhibition of morphine dependence by a lipopolysaccharide from pantoea agglomerans. | a lipopolysaccharide from pantoea agglomerans (lpsp) was purified and examined for relief of morphine dependence by observing its inhibition of the jumping of mice on naloxone-precipitate withdrawal. administration of lpsp either intravenously or intradermally showed marked inhibition of the jumping. beta-endorphin in mouse serum and brain tissue were recognized to be in synchrony with the time course of the relief. administration of tnf-alpha gave similar effect, suggesting that lpsp induces a ... | 1992 | 1421014 |
| the agglutinin response to whole-cell and acellular pertussis vaccines is bordetella pertussis--strain dependent. | to determine the significance of the bordetella pertussis strain used as the antigen in the agglutinin assay for the evaluation of pertussis vaccines. | 1992 | 1415040 |
| homeostasis as regulated by activated macrophage. vi. protective effect of lpsw (a lipopolysaccharide from wheat flour) against acute infection by toxoplasma gondii in mice. | an oral administration of partially purified lpsw, a lipopolysaccharide (lps) from wheat flour, at a concentration of 20 ng/ml in drinking water beginning 1d after infection significantly decreased mouse mortality and prevented animal weight loss in acute infection with toxoplasma gondii. whereas 71% (5/7) of mice in a control group that did not receive lpsw died of toxoplasmosis, only 14% (1/7) of mice treated with lpsw died (p less than 0.05). the administration of lps purified from bordetella ... | 1992 | 1394645 |
| high-affinity calmodulin binding is required for the rapid entry of bordetella pertussis adenylyl cyclase into neuroblastoma cells. | bordetella pertussis produces a calmodulin-stimulated adenylyl cyclase that invades animal cells and raises intracellular camp levels [confer, d. l., & eaton, j. w. (1982) science 217, 948-950; shattuck, r. l., & storm, d. r. (1985) biochemistry 24, 6323-6328]. the mechanism for invasion of animal cells by this enzyme has not been defined, but there is considerable evidence that it does not enter by receptor-mediated endocytosis [gordon, v. m., leppla, s. h., & hewlett, e. l. (1988) infect. immu ... | 1992 | 1390675 |
| the relationship between late asthmatic responses and antigen-specific immunoglobulin. | the aim of this study was to examine the relationships between allergen-induced early and late airway responses and antigen-specific ige, igg, and lymphocyte subsets in blood and bronchoalveolar lavage (bal). brown norway rats were sensitized at 7 weeks of age with ovalbumin (1 mg s.c.) with use of bordetella pertussis as an adjuvant. three weeks after sensitization, animals were anesthetized and challenged with an aerosol of ovalbumin (5% wt/vol in saline) for 5 minutes. each animal was studied ... | 1992 | 1383308 |
| pertussis whole cell vaccine: relation between intracerebral protection in mice and antibody response to pertussis toxin, filamentous hemagglutinin and adenylate cyclase. | n:nih mice were vaccinated according to the who recommendations for the potency test with the second international standard for pertussis vaccine (ispv). blood for serological investigation was taken from the animals on day 14 post immunization before intracerebral challenge with bordetella pertussis 18323 was done. the relationship between anti-pertussis toxin, anti-filamentous hemagglutinin and anti-adenylate cyclase antibody levels as measured by elisa and protection from intracerebral challe ... | 1992 | 1520970 |
| multiple discontinuity as a remarkable feature of the development of acellular pertussis vaccines. | development of the japanese acellular pertussis vaccines (apvs) of the 1980s involved six procedural or conceptual features that were discontinuous with the then-accepted views of how pertussis vaccines should be made and tested. these discontinuities were: modification of the standard intracerebral mouse test for protective potency; use of culture supernates, rather than cells, of bordetella pertussis as the feedstock for antigen purification; use of haemagglutination as a measure of protective ... | 1992 | 1523873 |
| homeostasis as regulated by activated macrophage. iii. protective effect of lpsw (lipopolysaccharide (lps) of wheat flour) on gastric ulcer in mice as compared with those of other lps from various sources. | protective effect of lipopolysaccharide (lps) from various sources on gastric ulcer has been examined in mice using parenteral as well as oral route. ulcer is induced by indomethacin, stress or alcohol. lps was prepared from 6 species of bacteria (escherichia coli, pantoea agglomerans, serratia ficaria, enterobacter cloacae, bordetella pertussis, alcaligenes faecalis) and from wheat flour. when administered intravenously, lps of pantoea agglomerans was the most effective among other lps examined ... | 1992 | 1525959 |
| the longevity of the immune response to filamentous hemagglutinin and pertussis toxin in patients with pertussis in a semiclosed community. | titers of antibodies to the filamentous hemagglutinin (fha) and pertussis toxin (pt) of bordetella pertussis were studied in patients from a semiclosed community after an outbreak of the disease in 1985 and in the general population. in convalescent-phase serum obtained after b. pertussis infection in patients in the semiclosed community, high anti-fha igg and anti-pt igg titers were observed; these titers tended to disappear over the next 5 years. on the other hand, among the medical staff, hig ... | 1992 | 1527428 |
| cloning, nucleotide sequence and heterologous expression of the protective outer-membrane protein p.68 pertactin from bordetella bronchiseptica. | the prn gene encoding the 68 kda protective outer-membrane protein of bordetella bronchiseptica (p.68 pertactin) was cloned, sequenced and expressed in escherichia coli. the gene was isolated by dna:dna hybridization experiments using a radioactively-labelled fragment of the homologous prn gene from bordetella parapertussis. dna sequence analysis reveals that the gene is capable of encoding a protein with a molecular mass of 93996 da (p.94); this precursor molecule is processed to form the p.68 ... | 1992 | 1527510 |
| impact of pertussis on the afflicted child and family. | the effects of pertussis in an unimmunized population were investigated in 61 children consecutively identified by positive culture for bordetella pertussis. parents were interviewed twice, at a mean of 3 and 11 weeks after onset of symptoms. all children had a disease duration of more than 3 weeks. behavioral changes were reported for 84% of the study children. parents reported negative effects for themselves in 95% and for siblings in 63% of the families. the main problems were disturbance of ... | 1992 | 1528646 |
| lymphocyte migration in the mouse. i. time course of cell accumulation and the effect of antigen sensitisation and challenge in a murine model of chronic inflammation. | the effect of time and of antigen sensitisation and challenge on lymphocyte migration into a site of chronic inflammation has been examined in the mouse. enhanced lymphocyte migration occurred at sites of chronic inflammation after sensitisation and challenge to bordetella pertussis vaccine (bpv). biphasic migration was observed with time (5 min to 24 h), the initial very rapid but transient localisation at the inflamed site being followed by a second slower more sustained influx of cells. incre ... | 1992 | 1382369 |
| effect of pertussis toxin on susceptibility of infant rats to haemophilus influenzae type b. | pertussis toxin is an important virulence factor of bordetella pertussis that may also contribute to the toxicity of pertussis vaccines. the effect of low doses of pertussis toxin on response to haemophilus influenzae type b (hib) infection was examined in infant rats. pretreatment of rats with 10 or 100 ng of pertussis toxin increased blood bacterial concentration (p less than .01), serum endotoxin levels (p less than .01), and mortality (p less than .05) relative to saline pretreated controls ... | 1992 | 1533239 |
| the monoclonal antibody mel-14 can block lymphocyte migration into a site of chronic inflammation. | the effect of mel-14, a monoclonal antibody which binds to the lymphocyte homing receptor (mel-14 ag) on lymphocytes for peripheral lymph node (pln) high endothelial venules (hev), was investigated on lymphocyte migration into a delayed-type hypersensitivity (dth)-like lesion produced by sensitization and challenge to bordetella pertussis vaccine (bpv). pretreatment of lymphocytes with saturating concentrations of mel-14 caused a highly significant inhibition of lymphocyte migration into the chr ... | 1992 | 1534762 |
| recombinant p.69/pertactin: immunogenicity and protection of mice against bordetella pertussis infection. | the immunogenicity of recombinant (r-) pertactin was examined. parenteral immunization of mice with natural or r-pertactin produced a similar increase in serum anti-pertactin antibodies and a decrease in bordetella pertussis lung counts following aerosol challenge. study of the kinetics of b. pertussis growth in the respiratory tract of immunized and control mice revealed that immunization with r-pertactin halted the multiplication of b. pertussis in the lungs and facilitated the early onset of ... | 1992 | 1539459 |
| dna probes for bordetella species and a colorimetric reverse hybridization assay for the detection of bordetella pertussis. | three oligonucleotide probe sequences were inferred from the 16s ribosomal ribonucleic acid (rrna) and the 16s-23s rrna spacer sequences of bordetella pertussis atcc 10380. these probes were used in hybridization tests with deoxyribonucleic acid from bordetella species and other relevant bacterial taxa. a probe from the spacer region hybridized exclusively to the b. pertussis strains tested and not to strains from other species. using a combination of three probes, b. pertussis, b. parapertussis ... | 1992 | 1382221 |
| effects of guanidinium hydrochloride on the structure and immunological properties of bordetella pertussis fimbriae. | denaturation of bordetella pertussis fimbrial preparations by guanidinium hydrochloride (gdnhcl) has been characterized using static light scattering, c.d., fluorescence and antibody recognition. the susceptibility of fim2 + 3 (a mixed preparation of two fimbrial types) to gdnhcl was found to be highly dependent on ph; as the ph was increased from ph 7.2 to 10.5, the concentration of gdnhcl required to induce 50% denaturation was decreased. at ph 10.5, fim2 + 3 was denatured by gdnhcl in a three ... | 1992 | 1375451 |
| immunochemical localization of a region of chaperonin-60 important for productive interaction with chaperonin-10. | an igg1 monoclonal antibody (mab 54g8) which binds to both bordetella pertussis chaperonin-60 (cpn60) and escherichia coli cpn60 (groel) was produced. mab 54g8 as well as fab fragments prepared from this antibody were found to abolish the ability of chaperonin-10 (cpn10, groes) to inhibit the atpase activity of both b. pertussis cpn60 and e. coli cpn60. electron microscopy was used to localize the binding site of the monoclonal antibody on the b. pertussis cpn60 molecule. in the absence of the a ... | 1992 | 1361184 |
| characterization of a bordetella pertussis fimbrial gene cluster which is located directly downstream of the filamentous haemagglutinin gene. | the biosynthesis of fimbriae is a complex process requiring multiple genes which are generally found clustered on the chromosome. in bordetella pertussis, only major fimbrial subunit genes have been identified, and no evidence has yet been found that they are located in a fimbrial gene cluster. to locate additional genes involved in the biosynthesis of b. pertussis fimbriae, we used tnphoa mutagenesis. a phoa+ mutant (designated b176) was isolated which was affected in the production of both ser ... | 1992 | 1360139 |
| somatostatin inhibition of phosphoinositides turnover in isolated rat acinar pancreatic cells: interaction with bombesin. | the effects of somatostatin-14 and bombesin on [3h]inositol phosphate accumulation were studied in 24 h myo-[3h]inositol-prelabeled cultured rat acinar cells. bombesin, 10 nm, stimulated basal formation of phosphatidyl monophosphate (insp1), phosphatidyl 4,5-biphosphate (insp2) and inositol 1,4,5-triphosphate (insp3) by 128 +/- 5.2%, 147 +/- 10% and 155 +/- 5%, respectively. at 5 s, the ed50 value for insp3 stimulation was 0.70 +/- 0.2 nm. this stimulation was partly blocked (64 +/- 0.04% inhibi ... | 1992 | 1359613 |
| [molecular and cellular bases of the virulence of shigella flexneri]. | shigella flexneri, a gram negative bacillus, causes bacillary dysentery, an ulcerative disease of the human colon, by invading intestinal epithelial cells. entry into epithelial cells occurs via an induced phagocytic process which involves the actino-myosin complex. the host-cell receptor and the transmembrane signal which initiate reorganization of the cytoskeleton are under study. binding to integrins has recently been demonstrated in related models such as the entry of yersinia pseudotubercul ... | 1992 | 1554236 |
| a monoclonal antibody directed against the catalytic site of bacillus anthracis adenylyl cyclase identifies a novel mammalian brain catalytic subunit. | a brain adenylyl cyclase was shown to contain an epitope closely related to that specified by a conserved sequence containing a nucleotide-binding consensus sequence gxxxxgks and located in the catalytic sites of bacterial, calmodulin-dependent adenylyl cyclases [goyard, s., orlando, c., sabatier, j.-m., labruyere, e., d'alayer, j., fontan, g., van rietschoten, j., mock, m., danchin, a., ullmann, a., & monneron, a. (1989) biochemistry 28, 1964-1967]. a monoclonal antibody, mab 164, produced agai ... | 1992 | 1554706 |
| common accessory genes for the bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papc and papd gene families. | the bordetella pertussis filamentous hemagglutinin (fha) is a major virulence factor responsible for attachment, one of the early events in bacterial pathogenesis. deletion of its structural gene, fhab, or a tn5 insertion in fhaa, downstream of fhab, resulted in a fha- and fimbriae- phenotype, although fhab and the fim genes are not linked. the fhab downstream region therefore most likely encodes accessory proteins required for the biosynthesis of fha and fimbriae, despite the lack of sequence s ... | 1992 | 1354611 |
| agglutination of bordetella species by lectins. | phase i cells of bordetella pertussis but not those of b. parapertussis, b. bronchiseptica or b. avium were agglutinated by limulus polyphemus lectin. most strains of b. pertussis but not those of the other species were also agglutinated by helix pomatia lectin. in precipitation reactions between lectins and purified bordetella lipopolysaccharide (lps) preparations a similar pattern occurred. lectin agglutination provides a rapid presumptive method for the differentiation of b. pertussis from b. ... | 1992 | 1555759 |
| potency testing of acellular pertussis vaccines. | the laboratory assessment of the potential of acellular pertussis vaccines to protect against human disease is a major problem. the mouse intracerebral challenge test, which is the accepted potency assay for whole cell pertussis vaccines, is not suitable for testing acellular vaccines, and more recently developed murine respiratory infection assays have methodological drawbacks and doubtful relevance to the human infection. we have found that the ability of several bordetella pertussis antigens ... | 1992 | 1557927 |
| role of carbohydrate recognition domains of pertussis toxin in adherence of bordetella pertussis to human macrophages. | pertussis toxin (pt) and filamentous hemagglutinin can each mediate the association of bordetella pertussis with human macrophages. adherence via filamentous hemagglutinin leads to integrin-mediated entry and survival of the bacteria within the human cell. we determined the contribution of pt to bacterial adherence to human macrophages. plating macrophages on wells coated with recombinant pt subunit 2 (s2) or s3 decreased pt-dependent bacterial binding by greater than 60%; s1, s4, and s5 were in ... | 1992 | 1353482 |
| memory cell generation ablated by soluble protein antigen by means of effects on t- and b-lymphocyte compartments. | adult c57bl/6 mice were injected with 100 micrograms of soluble, freshly deaggregated human serum albumin (hsa) to produce partial immunologic tolerance. uninjected normal control (n) mice contain only approximately 100 b cells in their spleens with the capacity to (i) be activated in vitro into clonal proliferation by escherichia coli lipopolysaccharide plus interleukins 2, 4, and 5, (ii) form igg1 as well as igm antibody, and (iii) display specificity for hsa when only igg1 is allowed to score ... | 1992 | 1348366 |
| immune response in mice immunized with acidic antigenic fractions from trypanosoma cruzi cytosol. | the humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of t. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, f iii and f iv, plus bordetella pertussis as adjuvant. the immunization with f iii induced positive ith and dth responses to homologous antigens. in mice immunized with f iv, the ith was negative and four out of six animals presented positive dth reactions. in both groups of mice the analysis of i ... | 1992 | 1342100 |