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[meningitis due to kingella kingae]. 200717583630
molecular diagnosis of kingella kingae pericarditis by amplification and sequencing of the 16s rrna gene.kingella kingae is a fastidious gram-negative bacillus that is considered an emerging pathogen in pediatric settings but remains less common in adults. here we describe a case of pericarditis in an immunocompetent adult host. the microorganism was identified directly from the clinical sample by molecular techniques, i.e., 16s rrna gene amplification and sequencing.200717634294
bacterial peritonitis caused by kingella kingae.kingella kingae is a commensal of the upper respiratory tract that occasionally causes skeletal infections in children and endocarditis in children and adults. we report a case of a 55-year-old man with liver disease and tense ascites who performed a paracentesis on himself and developed k. kingae peritonitis and bacteremia.200717634309
kingella kingae endocarditis in a sixteen-month-old-child.we report the case of a child with acute neurologic symptoms who was found to have bacterial endocarditis caused by kingella kingae. the case alerts microbiologists and pediatricians to an organism that has rarely been reported to cause endocarditis in children.200111332678
bacterial endocarditis due to eikenella corrodens: a case report.of all the causes of bacterial endocarditis, hacek group consisting of haemophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae are rare causative agents. we report a case of bacterial endocarditis by e. corrodens, which is one of the members of the hacek group.200317643001
septic arthritis due to kingella kingae in an adult. 200111235790
cardiobacterium hominis endocarditis: a case report and review of the literature.the present case report describes the clinical course of a patient who presented with cardiobacterium hominis endocarditis. a review of the literature follows the case presentation. c hominis, a fastidious gram-negative bacillus, is a member of the hacek group of microorganisms (haemophilus species, actinobacillus actinomycetemcomitans, c hominis, eikenella corrodens and kingella kingae). endocarditis caused by c hominis is uncommon and generally follows a subacute course. patients may present w ...200518159562
infective endocarditis due to haemophilus aphrophilus: a case report.to report the case of a child with infective endocarditis caused by haemophilus aphrophilus.200818213436
staphylococcus aureus bacteremia complicating herpes simplex virus type 1 stomatitis: case report and review of the literature.gingivostomatitis is a common clinical manifestation of primary herpes simplex virus type 1 (hsv-1) infection in children. the most common complication of herpetic gingivostomatitis is dehydration; rarely, it may be complicated by secondary bacteremia, and kingella kingae and group a streptococcus infections have been reported to be responsible for such episodes.200818251654
two cases of diskitis attributable to anaerobic bacteria in children.diskitis, an inflammation of the intervertebral disk, is generally attributable to staphylococcus aureus and rarely staphylococcus epidermidis, kingella kingae, enterobacteriaciae, and streptococcus pneumoniae. in many cases, no bacterial growth is obtained from infected intervertebral discs. although anaerobic bacteria were recovered from adults with spondylodiscitis, these organisms were not reported before from children. the recovery of anaerobic bacteria in 2 children with diskitis is report ...200111158500
[endocarditis due to aggregatibacter (formerly: actinobacillus) actinomycetemcomitans, a bacterium that grows in characteristic star-shaped colonies].a 72-year-old man, having had an artificial valve for almost 20 years now, presented with tiredness that had persisted for several weeks and reported weight loss of 5 kg. in more recent days he experienced fever and cold shivers, and an associated dry cough. bearing in mind the potential for endocarditis, blood cultures were grown. in this, we identified a small, gram-negative rod with a small, smooth, raised colony that grew slowly. we considered a micro-organism from the 'hacek group', which i ...200818491827
direct comparison of the bd phoenix system with the microscan walkaway system for identification and antimicrobial susceptibility testing of enterobacteriaceae and nonfermentative gram-negative organisms.the phoenix automated microbiology system (bd diagnostics, sparks, md) is designed for the rapid identification (id) and antimicrobial susceptibility testing (ast) of clinically significant human bacterial pathogens. we evaluated the performance of the phoenix instrument in comparison with that of the microscan walkaway system (dade behring, west sacramento, ca) in the id and ast of gram-negative clinical strains and challenge isolates of enterobacteriaceae (n = 150) and nonfermentative gram-neg ...200818495856
usefulness of broad-range pcr for the diagnosis of osteoarticular infections.conventional methods such as microbiological cultures may lack the sensitivity and specificity to establish definitive diagnosis of osteoarticular infections. herein, we review the general principles and the usefulness of broad-range pcr to improve the etiological diagnosis of osteoarticular infections.200818525362
streptobacillus moniliformis as the causative agent in spondylodiscitis and psoas abscess after rooster scratches.we report a case of streptobacillus moniliformis spondylodiscitis accompanied by a psoas abscess in an 80-year-old man scratched by a rooster. s. moniliformis was identified from abscess fluid by use of 16s rrna gene sequencing. after 18 weeks of antimicrobial therapy, the clinical condition of the patient improved.200818562588
isolation of kingella kingae from synovial fluids using four commercial blood culture bottles.according to the literature, kingella kingae may be an underdiagnosed cause of joint and bone infections in children. the use of the bactec blood culture system for culture of joint fluids has dramatically improved the isolation of this fastidious bacterium. the aim of this study was to test the recovery rate and detection time of four commercial blood culture systems: three different bact/alert (organon teknika, usa) bottles and one bactec (becton dickinson microbiology systems, usa) bottle, al ...200011014623
osteoarticular infections in belgian children: a survey of clinical, biological, radiological and microbiological data.the aim of this study is to report the pathogens which were found most frequently to be responsible for osteo-articular infections in infants and children in belgium, and to propose an appropriate empirical antibiotic therapy applicable before identification of the responsible pathogen. clinical presentation, imaging and blood biology are also reviewed and analysed. fifty-six cases of osteo-articular infections (acute/subacute osteomyelitis, osteo-arthritis, septic arthritis, spondylodiscitis, s ...200818686465
[pediatric osteoarticular infections caused by kingella kingae from 1995 to 2006 at chru de tours].use of molecular biology shows that kingella kingae is a pathogen frequently involved in osteoarticular infections in young children. this study describes the cases of osteoarticular infections due to k. kingae which happened from 1995 to 2006 in the chru of tours. the description is based on clinical and biological features. a k. kingae specific polymerase chain reaction was performed in our laboratory in order to improve k. kingae osteoarticular infections diagnosis, and is detailed here.200818725349
kingella kingae expresses type iv pili that mediate adherence to respiratory epithelial and synovial cells.kingella kingae is a gram-negative bacterium that colonizes the respiratory tract and is a common cause of septic arthritis and osteomyelitis. despite the increasing frequency of k. kingae disease, little is known about the mechanism by which this organism adheres to respiratory epithelium and seeds joints and bones. previous work showed that k. kingae expresses long surface fibers that vary in surface density. in the current study, we found that these fibers are type iv pili and are necessary f ...200818757541
trimethoprim-sulfamethoxazole for osteoarthritis caused by staphylococcus aureus or kingella kingae. 200818818554
invasive kingella kingae infections in children: clinical and laboratory characteristics.kingella kingae, a gram-negative coccobacillus, is being increasingly recognized as an invasive pathogen in children, causing mainly bacteremia and arthritis; however, there have been only a few studies on k kingae infections to date, mostly small-scale series. the aim of this study was to report our experience with invasive k kingae infections in children who were hospitalized at a major tertiary medical center in israel.200819047250
molecular diagnosis of kingella kingae osteoarticular infections by specific real-time pcr assay.kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. the bacterium is best detected by rapid inoculation in blood culture systems or by real-time pcr assays. pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as rtx, which is toxic to a variety of human cell types. the locus encoding the rtx toxin is thought to be a putative virulence factor, and is, appa ...200919074654
infective arthritis: bacterial 23s rrna gene sequencing as a supplementary diagnostic method.consecutively collected synovial fluids were examined for presence of bacterial dna (a 700-bp fragment of the bacterial 23s rrna gene) followed by dna sequencing of amplicons, and by conventional bacteriological methods. one or more microorganisms were identified in 22 of the 227 synovial fluids (9,7%) originating from 17 patients. sixteen of the patients had clinical signs of arthritis. for 11 patients molecular and conventional bacterial examinations were in agreement. staphylococcus aureus, s ...200819088916
pharyngeal colonization by kingella kingae in children with invasive disease.kingella kingae organisms isolated from the blood of 3 children with invasive infections were identical by pulsed field gel electrophoresis and random amplified polymorphic dna-polymerase chain reaction analysis to those recovered from the patients' pharynx, demonstrating the likely role of upper respiratory tract colonization in the pathogenesis of the disease caused by this bacterium.200919106774
kingella kingae: osteoarticular infections of the sternum in children: a report of six cases.kingella kingae is increasingly recognized as a pathogen of osteoarticular infections (oai) below the age of 2 years. it was reported that bones and joints which are rarely infected by other pathogens were frequently invaded by k. kingae. based on a series of six cases, we present the typical clinical and paraclinical manifestation of k. kingae infections of the sternum and sterno-manubrial joint.200819308540
new real-time pcr-based method for kingella kingae dna detection: application to samples collected from 89 children with acute arthritis.inoculation of blood culture vials with joint fluid samples has revealed the important pathogenic role of kingella kingae in pediatric arthritis. however, recent studies based on broad-range 16s ribosomal dna pcr and real-time pcr without a probe suggest that conventional methods remain suboptimal. we developed a new real-time pcr method with a probe that is highly specific for k. kingae and applied it to joint fluid samples collected from 89 children with suspected arthritis admitted to our ins ...200919369442
diversity and site-specificity of the oral microflora in the elderly.the purpose of the present study was to describe the bacterial diversity in the oral cavity of the elderly without root caries using bacterial microarrays, and to determine the site- and subject-specificity of bacterial colonization. samples were collected from the tongue dorsum, mucosa of the buccal fold, hard palate, supragingival plaque from sound root surfaces, and subgingival plaque from the same roots. a new 16 s rrna gene-based microarray method was used for the simultaneous detection of ...200919373498
[usefulness of synovial fluid inoculation in blood culture bottles for diagnosing kingella kingae septic arthritis: state of the question]. 200919409667
expression of kingella kingae type iv pili is regulated by sigma54, pils, and pilr.kingella kingae is a member of the neisseriaceae and is being recognized increasingly as an important cause of serious disease in children. recent work has demonstrated that k. kingae expresses type iv pili that mediate adherence to respiratory epithelial and synovial cells and are selected against during invasive disease. in the current study, we examined the genome of k. kingae strain 269-492 and identified homologs of the rpon and the pils and pilr genes that are essential for pilus expressio ...200919465661
dissemination of kingella kingae in the community and long-term persistence of invasive clones.although kingella kingae is being increasingly recognized as an important pediatric pathogen, our current understanding of the transmission of the organism is limited. the dissemination of k. kingae in the community was studied in 2 ethnic groups living side-by-side in southern israel.200919593253
kingella kingae prosthetic valve endocarditis complicated by a paravalvular abscess. 200919603602
comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray.this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim).200919722792
effects of preincubation temperature on the detection of fastidious organisms in delayed-entry samples in the bact/alert 3d blood culture system.this study evaluates the effect of preincubation on delayed-entry samples for fastidious organisms including the hacek group, streptococcus species, neisseria meningitidis, haemophilus species and corynebacterium species for the bact/alert 3d system (biomérieux) using the fa (aerobic) medium. bottles were inoculated with two different concentrations (0.5 mcfarland and a 1:100,000 dilution) of each organism and either loaded into the system immediately or stored at 4 degrees c, room temperature ( ...200919733598
osteomyelitis/septic arthritis caused by kingella kingae among day care attendees--minnesota, 2003.kingella kingae is a fastidious gram-negative coccobacillus that colonizes the respiratory and oropharyngeal tract in children. k. kingae occasionally causes invasive disease, primarily osteomyelitis/septic arthritis in young children, bacteremia in infants, and endocarditis in school-aged children and adults. although diagnosis of this organism frequently is missed, invasive disease is uncommon. only sporadic, non-epidemiologically linked cases have been reported previously. in october 2003, th ...200415041952
an adult case of urinary tract infection with kingella kingae: a case report.kingella kingae, though part of the normal upper respiratory tract and genitourinary tract, is increasingly being recognized as an important human pathogen. during the past decade, it has emerged as a significant pathogen in the pediatric age group primarily causing bacteremia and osteoarticular infections. adult infection usually occurs in individuals who are severely immunocompromised and most infections have taken the form of septicemia or septic arthritis. bacteremia due to k. kingae has bee ...200919830146
acute haemophilus parainfluenzae endocarditis: a case report.numerous pathogens can cause infective endocarditis, including haemophilus parainfluenzae. h. parainfluenzae is part of the h. aphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae group that may cause about 3% of the total endocarditis cases, and is characterized by a subacute course and large vegetations.200919830211
kingella kingae endocarditis and a cluster investigation among daycare attendees.kingella kingae can cause invasive pediatric infections and outbreaks of osteomyelitis/septic arthritis in daycare facilities have been described. this is the first reported public health investigation prompted by a case of k. kingae endocarditis in an infant attending a daycare facility. a concurrent case of osteomyelitis was identified. screening of daycare contacts revealed a low rate of colonization before antibiotic prophylaxis.201019884874
[pediatric osteo-articular infections with negative culture results: what about kingella kingae?].kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. the bacterium is best detected by rapid inoculation in blood culture systems or by real-time pcr assays. pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as rtx, which is toxic to a variety of human cell types. the locus encoding the rtx toxin is thought to be a putative virulence factor, and is, appa ...200919994673
[isolated bacteraemia caused by kingella kingae]. 201020006566
crystal structure analysis reveals pseudomonas pily1 as an essential calcium-dependent regulator of bacterial surface motility.several bacterial pathogens require the "twitching" motility produced by filamentous type iv pili (t4p) to establish and maintain human infections. two cytoplasmic atpases function as an oscillatory motor that powers twitching motility via cycles of pilus extension and retraction. the regulation of this motor, however, has remained a mystery. we present the 2.1 a resolution crystal structure of the pseudomonas aeruginosa pilus-biogenesis factor pily1, and identify a single site on this protein r ...201020080557
crystal structure analysis reveals pseudomonas pily1 as an essential calcium-dependent regulator of bacterial surface motility.several bacterial pathogens require the "twitching" motility produced by filamentous type iv pili (t4p) to establish and maintain human infections. two cytoplasmic atpases function as an oscillatory motor that powers twitching motility via cycles of pilus extension and retraction. the regulation of this motor, however, has remained a mystery. we present the 2.1 a resolution crystal structure of the pseudomonas aeruginosa pilus-biogenesis factor pily1, and identify a single site on this protein r ...201020080557
management of acute hematogenous osteomyelitis in children.in children, osteomyelitis is primarily hematogenous in origin and acute in nature. the principal cause of osteomyelitis in children is staphylococcus aureus, and both the epidemiology and pathogenesis of s. aureus infections, including osteomyelitis, have changed in recent years owing to the emergence of community-associated methicillin-resistant s. aureus. this review focuses on advances in the diagnosis and overall management of acute hematogenous osteomyelitis in children with these changes ...201020109047
kingella kingae infections in children--united states, june 2001-november 2002.kingella kingae is recognized increasingly as a cause of skeletal infections in children. recent studies indicate that direct inoculation of clinical specimens into aerobic blood culture bottles (abcbs), instead of direct plating of specimens on solid media, might improve recovery of the fastidious bacteria. prompted by a report of a possible cluster of osteoarticular infections caused by k. kingae among children, the infectious diseases society of america emerging infections network (idsa-ein) ...200415041953
[discitis caused by kingella kingae--case report].the patient with acute infection and the presence of bacterium kingella kingae cultured from cerebrospinal fluid is presented. the analysis of clinical picture and the results of all performed tests did not allow to diagnose cerebrospinal meningitis. despite of being not sure if kingella kingae was an etiopathogenetic factor in the described subject, we decided to discuss this interesting case.200315049217
filifactor alocis--involvement in periodontal biofilms.bacteria in periodontal pockets develop complex sessile communities that attach to the tooth surface. these highly dynamic microfloral environments challenge both clinicians and researchers alike. the exploration of structural organisation and bacterial interactions within these biofilms is critically important for a thorough understanding of periodontal disease. in recent years, filifactor alocis, a fastidious, gram-positive, obligately anaerobic rod was repeatedly identified in periodontal les ...201020193074
rapid differentiation of francisella species and subspecies by fluorescent in situ hybridization targeting the 23s rrna.francisella (f.) tularensis is the causative agent of tularemia. due to its low infectious dose, ease of dissemination and high case fatality rate, f. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. microbiological diagnosis is cumbersome and time-consuming. methods for the direct detection of the pathogen (immunofluorescence, pcr) have been developed but are restricted to reference laboratories.201020205957
kingella kingae osteoarticular infections in young children: clinical features and contribution of a new specific real-time pcr assay to the diagnosis.kingella kingae is an emerging pathogen that may be recognized as the most common bacteria responsible for osteoarticular infections (oai) in young children. however, its diagnosis remains a challenge and thus little evoked in infants, because k. kingae is a difficult germ to isolate on solid medium, and clinical signs are often mild. the main objective of this prospective study is to describe the clinical, biologic, and radiologic features of children with oai caused by k. kingae. in addition, ...201020357599
subtle bacterial endocarditis due to kingella kingae in an infant: a case report.a 9-month-old infant presented with fever, dyspnoea, and a murmur. echocardiography showed a mitral vegetation with significant regurgitation. mitral valve plasty was performed on day 6, and was polymerase chain reaction positive for kingella kingae. the cardiac outcome was favourable. this case illustrates a subtle presentation of k. kingae mitral valve infective endocarditis in a normal-cardaic infant, treated with early surgery, and the agent belonged to the hacek (haemophilus spp actinobacil ...201020465860
[sacroiliitis due to kingella kingae in an adult: updates on this pathogen].we report here the case of a sacro-iliitis due to kingella kingae in a 35 year-old patient. this case report points out the pathogenic potency in adults of this organism which is known as a cause of invasive infections in young children. inoculation of blood culture vials with clinical specimens and the use of nucleic acid amplification have recently improved the sensitivity to identify kingella kingae and to diagnose infections which need an efficient antimicrobial therapy.201020478779
evaluation of the analytical performance of the xpert mtb/rif assay.we performed the first studies of analytic sensitivity, analytic specificity, and dynamic range for the new xpert mtb/rif assay, a nucleic acid amplification-based diagnostic system that detects mycobacterium tuberculosis and rifampin (rif) resistance in under 2 h. the sensitivity of the assay was tested with 79 phylogenetically and geographically diverse m. tuberculosis isolates, including 42 drug-susceptible isolates and 37 rif-resistant isolates containing 13 different rpob mutations or mutat ...201020504986
first report of septic arthritis caused by klebsiella oxytoca.klebsiella oxytoca is known to be a pathogen in immunodeficient adults and children. here we report the first case of a k. oxytoca infection associated with spontaneous arthritis of the knee in a child with no history of immunosuppressive therapy or previous bacterial infections. despite an initial antibiotic treatment failure, a second treatment led to a cure of the infection with no joint sequelae.201020573877
[kingella kingae ostemyelitis and septic arthritis in paediatric patients. six cases from the department of pediatrics, national university hospital of iceland.]kingella kingae (k. kingae) is a gram negative rod most often associated with septic arthritis and osteomyelitis in children. infections caused by k. kingae had not been reported in iceland when six cases were diagnosed at the pediatric department at the national university hospital of iceland. in this report we describe those cases and review the literature.200017018942
infections of people with complement deficiencies and patients who have undergone splenectomy.the complement system comprises several fluid-phase and membrane-associated proteins. under physiological conditions, activation of the fluid-phase components of complement is maintained under tight control and complement activation occurs primarily on surfaces recognized as "nonself" in an attempt to minimize damage to bystander host cells. membrane complement components act to limit complement activation on host cells or to facilitate uptake of antigens or microbes "tagged" with complement fra ...201020930072
[kingella kingae spondilodiscitis in an adult]. 201021093187
matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16s rrna gene sequencing for identification of difficult-to-identify bacterial strains.conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16s rrna gene sequencing. maldi-tof ms might represent an alternative to 16s rrna gene sequencing.201021106794
bacterial endosymbiont localization in hyalesthes obsoletus, the insect vector of bois noir in vitis vinifera.one emerging disease of grapevine in europe is bois noir (bn), a phytoplasmosis caused by "candidatus phytoplasma solani" and spread in vineyards by the planthopper hyalesthes obsoletus (hemiptera: cixiidae). here we present the first full characterization of the bacterial community of this important disease vector collected from bn-contaminated areas in piedmont, italy. length heterogeneity pcr and denaturing gradient gel electrophoresis analysis targeting the 16s rrna gene revealed the presenc ...201021183640
bacterial endosymbiont localization in hyalesthes obsoletus, the insect vector of bois noir in vitis vinifera.one emerging disease of grapevine in europe is bois noir (bn), a phytoplasmosis caused by "candidatus phytoplasma solani" and spread in vineyards by the planthopper hyalesthes obsoletus (hemiptera: cixiidae). here we present the first full characterization of the bacterial community of this important disease vector collected from bn-contaminated areas in piedmont, italy. length heterogeneity pcr and denaturing gradient gel electrophoresis analysis targeting the 16s rrna gene revealed the presenc ...201021183640
diagnosis of e. coli tricuspid valve endocarditis: a case report.escherichia coli is a rare cause of bacterial endocarditis. we report a case of an elderly man with cirrhosis who developed bacterial endocarditis due to e.coli. we review the literature that summarizes a total of 41 cases, with a predominance of elderly women, diabetics, and patients with urinary tract infections and prosthetic heart valves. e.coli bacteremia may be mistakenly attributed to a more benign source such as urosepsis and overlooked as an indication of endocarditis. a delay in diagno ...201021225584
identification of hacek clinical isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry.matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry is a rapid and accurate tool for the identification of many microorganisms. we assessed this technology for the identification of 103 haemophilus parainfluenzae, aggregatibacter aphrophilus, aggregatibacter actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae (hacek) clinical isolates and 20 haemophilus influenzae clinical isolates. ninety-three percent of hacek organisms ...201121227988
the rtxa toxin gene of kingella kingae: a pertinent target for molecular diagnosis of osteoarticular infections.kingella kingae is an emerging osteoarticular pathogen in young children. its isolation by traditional culture methods remains difficult, underscoring the need to implement other diagnostic methods for its detection and identification, such as nucleic acid amplification tests. although the genome of this bacterium has not yet been sequenced, a toxin named rtx has been identified. the goal of this study was to develop sensitive, specific, and rapid molecular methods based on the rtxa toxin gene s ...201121248099
[pathognomonic presentation of kingella kingae infection].kingella kingae is an important and probably under-recognized pathogen in children. it has a predilection for bones, joints and vascular endothelium, but can also cause bacteraemia alone. we present a case of kingella kingae that manifested itself by presternal soft tissue infection, and suggest that infection of the lower sternum and the junction between the manubrium and the xyphoid process are typical, if not pathognomonic for the organism.201121276404
kingella kingae spondylodiscitis in a child.most osteoarticular infections in children are due to staphylococcus aureus. in this case, the isolation of kingella kingae by image guided disc aspiration resulted in modification and optimization of treatment. we take a look at a case of spondylodiscitis in a young child and review some of the current literature with regards to kingella kingae infections.200617065283
first report of performance of the versant ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae.we evaluated the analytical, work flow, and clinical performance of the versant ct/gc dna 1.0 assay (versant ct/gc assay, where "ct" represents chlamydia trachomatis and "gc" represents neisseria gonorrhoeae). the assay simultaneously detects chlamydia trachomatis and neisseria gonorrhoeae in swab and first-catch urine (fcu) specimens. the limit of detection (lod) was determined to be 342 copies/ml for c. trachomatis and 137 copies/ml for gc. the versant ct/gc assay detected 15 c. trachomatis se ...201121307209
kingella kingae: an emerging pathogen in young children.kingella kingae is being recognized increasingly as a common etiology of pediatric osteoarticular infections, bacteremia, and endocarditis, which reflects improved culture methods and use of nucleic acid-amplification techniques in clinical microbiology laboratories. k kingae colonizes the posterior pharynx of young children and is transmitted from child to child through close personal contact. day care attendance increases the risk for colonization and transmission, and clusters of k kingae inf ...201121321033
fastidious gram-negatives: identification by the vitek 2 neisseria-haemophilus card and by partial 16s rrna gene sequencing analysis.taxonomy and identification of fastidious gram negatives are evolving and challenging. we compared identifications achieved with the vitek 2 neisseria-haemophilus (nh) card and partial 16s rrna gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic characterization using 100 fastidious gram negative bacteria. seventy-five strains represented 21 of the 26 taxa included in the vitek 2 nh database and 25 strains represented related species not included in th ...201021347215
microbiological profile of haematogenous osteoarticular infections in children.introduction: staphylococcus aureus, kingella kingae and ß-hemolytic streptococcus are presently the most frequently identified bacteria in child haematogenous osteoarticular infection. objective: to determine the microbiological profile (bacteria and antibiotic susceptibility) of osteoarticular infections in a paediatric hospital, so as to adapt treatment protocols to the ecology of the bacteria isolated. patients and methods: prospective descriptive study, including children admitted for acute ...201121371961
cytotoxic effects of kingella kingae outer membrane vesicles on human cells.kingella kingae is an emerging pathogen causing osteoarticular infections in pediatric patients. electron microscopy of k. kingae clinical isolates revealed the heterogeneously-sized membranous structures blebbing from the outer membrane that were classified as outer membrane vesicles (omvs). omvs purified from the secreted fraction of a septic arthritis k. kingae isolate were characterized. among several major proteins, k. kingae omvs contained virulence factors rtxa toxin and pilc2 pilus adhes ...201121443941
kingella kingae spondylodiscitis in young children: toward a new approach for bacteriological investigations? a preliminary report.as the result of improved bacteriological techniques, kingella kingae is a slow-growing gram-negative coccobacillus that is emerging as an important cause of spondylodiscitis in children younger than 3 years of age. the high pharyngeal carrier rates of this slow-growing gram-negative coccobacillus combined with the low incidence of identified k. kingae infections is possibly explained by a low virulence of this bacterium. the use of specific real-time polymerase chain reaction (pcr) on blood sam ...201021455474
[kingella kingae infections in the pediatric age].kingella kingae infections have aroused great interest in the last few years because of the increasing number of identified cases. although considered an emerging pathogen, the increase in diagnosis of these infections can probably be explained by better knowledge of the bacteria, improved microbiological diagnostic techniques and greater awareness among clinicians. k. kingae is an aerobic cocobacillus with high tropism for osteoarticular tissue, endocardium, and vascular space. this pathogen ma ...201121458708
differentiating osteoarticular infections caused by kingella kingae from those due to typical pathogens in young children.osteoarticular infections caused by kingella kingae are characterized by mild-to-moderate clinical and biologic inflammatory signs that are different from those caused by gram-positive cocci. a combined score was built to find the best model to predict k. kingae osteoarticular infections by using the following 4 variables: body temperature <38°c, serum c-reactive protein <55 mg/l, white blood cell count <14,000/mm, and band forms <150/mm.201121494171
comparison of clinical and biologic features of kingella kingae and staphylococcus aureus arthritis at initial evaluation.we conducted a retrospective study comparing the presenting clinical and biologic features of 64 children who had septic arthritis caused by kingella kingae with 26 children who had septic arthritis caused by staphylococcus aureus. children with k. kingae septic arthritis were significantly younger than those with s. aureus septic arthritis. otherwise, there were no significant differences between the 2 groups with respect to fever, location, white blood cell count, synovial fluid cell count, c- ...201121552181
broad spectrum biofilm inhibition by kingella kingae exopolysaccharide.cell-free extracts prepared from kingella kingae colony biofilms were found to inhibit biofilm formation by aggregatibacter actinomycetemcomitans, klebsiella pneumoniae, staphylococcus aureus, s. epidermidis, candida albicans and k. kingae. the extracts evidently inhibited biofilm formation by modifying the physicochemical properties of the cell surface, the biofilm matrix, and the substrate. chemical and biochemical analyses indicated that the biofilm inhibition activity in the k. kingae extrac ...201121602333
the utility of matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) following introduction for routine laboratory bacterial identification.maldi-tof ms was evaluated prospectively in a diagnostic laboratory. 927 organisms were tested in triplicate; 2351/2781 (85%) species and 2681/2781 (96%) genus identifications were correct. known issues such as the misidentification of α-haemolytic streptococci as streptococcus pneumonia were easily corrected. identifications cost $aud0.45 per isolate and were available in minutes. maldi-tof ms is rapid, accurate and inexpensive.201121632894
antibiotic treatment for acute haematogenous osteomyelitis of childhood: moving towards shorter courses and oral administration.acute haematogenous osteomyelitis (ahom) of childhood usually affects the long bones of the lower limbs. although almost any agent may cause ahom, staphylococcus aureus is the most common bacterium, followed by streptococcus pneumoniae and, in some countries, salmonella spp. and kingella kingae. magnetic resonance imaging (mri) has improved the diagnostic accuracy of traditional radiography and scintigraphy. except for the pre-treatment diagnostic sample from bone before the institution of antib ...201121640559
identification and characterization of an rtx toxin in the emerging pathogen kingella kingae.kingella kingae is an emerging bacterial pathogen that is increasingly recognized as the causative agent of a variety of pediatric diseases, including septic arthritis and osteomyelitis. the pathogenesis of k. kingae disease is believed to begin with colonization of the upper respiratory tract. in the present study, we examined interactions between k. kingae and cultured respiratory epithelial cells and observed potent cytotoxicity, detected by both microscopy and lactic acid dehydrogenase (ldh) ...200717098895
[antibiotic therapy of bone and joint infections in children: recent changes].management of bone and joint infections in children associates early appropriate antibiotic therapy against staphylococcus aureus and kingella kingae and, if necessary, surgical drainage of abscess or septic arthritis. in 2007, the paediatric infectious diseases group of the french society of paediatrics (gpip) proposed guidelines for antibiotherapy in acute and non-complicated cases, with an intravenous therapy during 4 to 7days followed by oral therapy during 3weeks.201121733665
Epidemiology of Invasive Kingella kingae Infections in Two Distinct Pediatric Populations Cohabiting in One Geographic Area.The annual incidence of invasive Kingella kingae younger than 4 years of age was significantly higher in westernized Jews than in indigent Bedouins living side-by-side in southern Israel (12.21/100,000 and 5.83/100,000, respectively, (P<0.05). One K. kingae clone was over-represented among isolates from Jewish children, suggesting that differences in bacterial pathogenicity may contribute to the morbidity excess detected in this population group.201122146739
Simplifying the treatment of acute bacterial bone and joint infections in children.The treatment of acute hematogenous bone and joint infections of children - osteomyelitis (OM), septic arthritis (SA) and OM-SA combination (OM+SA) - has simplified over the past years. The old approach included months-long antibiotic treatment, started intravenously for at least a week, followed by oral completion of the course. Recent prospective randomized trials show that most cases heal with a total course of 3 weeks (OM, OM+SA) or 2 weeks (SA) of an appropriate antibiotic, provided the cli ...201122114963
kingella kingae endocardial abscess and cerebral infarction in a previously well immunocompetent child.kingella kingae is an emerging paediatric pathogen that most commonly is associated with relatively benign osteoarticular disease in children. this report concerns a 1-year-old child with kingella kingae endocarditis and perivalvular abscess complicated by septic cerebral emboli and osteomyelitis leading to long-term neurological sequelae, highlighting the capacity of this organism to cause severe invasive disease in children.200922190987
abiotrophia defectiva knee prosthesis infection: a case report.abstract:201121896178
can early mri distinguish between kingella kingae and gram-positive cocci in osteoarticular infections in young children?background: k. kingae is a common causative organism in acute osteoarticular infections (oais) in children under 4 years of age. differentiation between k. kingae and gram-positive cocci (gpc) is of great interest therapeutically. objective: our aim was to identify early distinguishing mri features of oais. materials and methods: thirty-one children younger than 4 years of age with oai underwent mri at presentation. of these, 21 were caused by k. kingae and ten by gpc. bone and soft tissue react ...201121909715
high frequency of tropheryma whipplei in culture-negative endocarditis."classical" whipple's disease (cwd) is caused by tropheryma whipplei and is characterized by arthropathy, weight loss, and diarrhea. t. whipplei infectious endocarditis (twie) is rarely reported, either in the context of cwd or as isolated twie without signs of systemic infection. the frequency of twie is unknown, and systematic studies are lacking. here, we performed an observational cohort study on the incidence of t. whipplei infection in explanted heart valves in two german university center ...201122135251
Kingella kingae endocarditis: A rare case of mitral valve perforation.Kingella kingae, a HACEK (Haemophilus parainfluenzae, Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, Cardiobacterium hominis, Eikenella corrodens, Kingella kingae) organism, is a common resident of the upper airway in children; it has been associated with endocarditis in children with pre-existing heart conditions. This case report describes K. kingae endocarditis leading to valvular damage in a previously healthy 18-month-old child. Our patient developed a K. kingae bactere ...201121976892
[espondilodiskitis caused by kingella kingae in children: a case report].during the last years, spondilodiskitis due to kingella kingae has been a new target of interest, since it is the second agent that causes non tuberculous espondilodiskitis in children, after staphylococcus aureus. its clinical presentation is very inespecific, sometimes with fever, abdominal or lumbar disconfort, nocturnal pain, altered walking and sedestation. images, culture methods and pcr (polymerase chain reaction) can be helpful for diagnosis, although in an important percent of the cases ...201122052404
Kingella kingae Infections in Children: An Update. 201122125036
[a tenacious lameness in a 2-year-old child].spondylodiscitis, a septic infection of the intervertebral disc spondylodiscitis, a septic infection of the intervertebral disc and adjacent vertebrae, is an unusual infection, mainly affecting children and elderly people. it is classically associated with tuberculosis, but other germs such as staphylococcus aureus, streptococcus pyogenes or mitis, and some even more unususal ones (e.g. kingella kingae), are often encountered in our countries. non tuberculous spondylodiscitis is found in approxi ...200717566390
gangliosides block aggregatibacter actinomycetemcomitans leukotoxin (ltxa)-mediated hemolysis.aggregatibacter actinomycetemcomitans is an oral pathogen and etiologic agent of localized aggressive periodontitis. the bacterium is also a cardiovascular pathogen causing infective endocarditis. a. actinomycetemcomitans produces leukotoxin (ltxa), an important virulence factor that targets white blood cells (wbcs) and plays a role in immune evasion during disease. the functional receptor for ltxa on wbcs is leukocyte function antigen-1 (lfa-1), a ß-2 integrin that is modified with n-linked car ...201022069577
Direct Detection of Staphylococcus Osteoarticular Infections by Use of Xpert MRSA/SA SSTI Real-Time PCR.We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. U ...201121998420
septic arthritis caused by kingella kingae: a case report. 201122737437
human immunodeficiency virus & cardiovascular risk.highly active antiretroviral therapy (haart) significantly changed the prevalence of the cardiovascular manifestations of human immunodeficiency virus (hiv)/aids. in developed countries, a 30 per cent reduction in the prevalence of cardiomyopathy and pericardial effusion was observed, possibly related to a reduction of opportunistic infections and myocarditis. in developing countries, however, where the availability of haart is limited, and the pathogenic impact of nutritional factors is signifi ...201122310821
the rebirth of culture in microbiology through the example of culturomics to study human gut microbiota.bacterial culture was the first method used to describe the human microbiota, but this method is considered outdated by many researchers. metagenomics studies have since been applied to clinical microbiology; however, a "dark matter" of prokaryotes, which corresponds to a hole in our knowledge and includes minority bacterial populations, is not elucidated by these studies. by replicating the natural environment, environmental microbiologists were the first to reduce the "great plate count anomal ...201525567229
diagnostic criteria and problems in infective endocarditis. 200415145855
rapid universal identification of bacterial pathogens from clinical cultures by using a novel sloppy molecular beacon melting temperature signature technique.a real-time pcr assay with the ability to rapidly identify all pathogenic bacteria would have widespread medical utility. current real-time pcr technologies cannot accomplish this task due to severe limitations in multiplexing ability. to this end, we developed a new assay system which supports very high degrees of multiplexing. we developed a new class of mismatch-tolerant "sloppy" molecular beacons, modified them to provide an extended hybridization range, and developed a multiprobe, multimelt ...200919923485
rapid universal identification of bacterial pathogens from clinical cultures by using a novel sloppy molecular beacon melting temperature signature technique.a real-time pcr assay with the ability to rapidly identify all pathogenic bacteria would have widespread medical utility. current real-time pcr technologies cannot accomplish this task due to severe limitations in multiplexing ability. to this end, we developed a new assay system which supports very high degrees of multiplexing. we developed a new class of mismatch-tolerant "sloppy" molecular beacons, modified them to provide an extended hybridization range, and developed a multiprobe, multimelt ...200919923485
a guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2013 recommendations by the infectious diseases society of america (idsa) and the american society for microbiology (asm)(a).the critical role of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between the physician and the microbiologists who provide enormous value to the health care team. this document, developed by both laboratory and clinical experts, provides information on which tests are valuable and in which contexts, and on tests that add little or no value for diagnostic decisions. sections are divided into anatomic systems, including bloodstream i ...201323845951
impact of calcium signaling during infection of neisseria meningitidis to human brain microvascular endothelial cells.the pili and outer membrane proteins of neisseria meningitidis (meningococci) facilitate bacterial adhesion and invasion into host cells. in this context expression of meningococcal pilc1 protein has been reported to play a crucial role. intracellular calcium mobilization has been implicated as an important signaling event during internalization of several bacterial pathogens. here we employed time lapse calcium-imaging and demonstrated that pilc1 of meningococci triggered a significant increase ...201425464500
neisseria meningitidis translation elongation factor p and its active-site arginine residue are essential for cell viability.translation elongation factor p (ef-p), a ubiquitous protein over the entire range of bacterial species, rescues ribosomal stalling at consecutive prolines in proteins. in escherichia coli and salmonella enterica, the post-translational β-lysyl modification of lys34 of ef-p is important for the ef-p activity. the β-lysyl ef-p modification pathway is conserved among only 26-28% of bacteria. recently, it was found that the shewanella oneidensis and pseudomonas aeruginosa ef-p proteins, containing ...201626840407
patient-specific analysis of periodontal and peri-implant microbiomes.periodontally involved teeth have been implicated as 'microbial reservoirs' in the etiology of peri-implant diseases. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the degree of congruence between adjacent peri-implant and periodontal microbiomes in states of health and disease. subgingival and peri-implant biofilm samples were collected from 81 partially edentulous individuals with periodontal and peri-implant health and disease. bacterial dna wa ...201324158341
molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis.the human palatine tonsils, which belong to the central antigen handling sites of the mucosal immune system, are frequently affected by acute and recurrent infections. this study compared the microbiota of the tonsillar crypts in children and adults affected by recurrent tonsillitis with that of healthy adults and children with tonsillar hyperplasia. an in-depth 16s rrna gene based pyrosequencing approach combined with a novel strategy that included phylogenetic analysis and detection of species ...201323437130
the influence of smoking on the peri-implant microbiome.smokers are at high risk for 2 bacterially driven oral diseases: peri-implant mucositis and peri-implantitis. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the effect of smoking on the peri-implant microbiome in states of health and disease. peri-implant biofilm samples were collected from 80 partially edentulous subjects with peri-implant health, peri-implant mucositis, and peri-implantitis. bacterial dna was isolated and 16s ribsomal rna gene li ...201526124222
reduced set of virulence genes allows high accuracy prediction of bacterial pathogenicity in humans.although there have been great advances in understanding bacterial pathogenesis, there is still a lack of integrative information about what makes a bacterium a human pathogen. the advent of high-throughput sequencing technologies has dramatically increased the amount of completed bacterial genomes, for both known human pathogenic and non-pathogenic strains; this information is now available to investigate genetic features that determine pathogenic phenotypes in bacteria. in this work we determi ...201222916122
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