| utility of extended blood culture incubation for isolation of haemophilus, actinobacillus, cardiobacterium, eikenella, and kingella organisms: a retrospective multicenter evaluation. | the incidence of and average time to detection for haemophilus, actinobacillus, cardiobacterium, eikenella, and kingella (hacek) bacteria in blood cultures with standard incubation and the utility of extended incubation of blood culture bottles were reviewed at four tertiary care microbiology laboratories. hacek organisms were isolated from 35 (<0.005%) of 59,203 positive blood cultures. none of 407 blood cultures with extended incubation grew hacek or other bacteria. bacteremia from hacek bacte ... | 2006 | 16390985 |
| kingella kingae: an emerging pathogen of acute osteoarticular infections in children. | | 2006 | 16396891 |
| direct detection of cardiobacterium hominis in serum from a patient with infective endocarditis by broad-range bacterial pcr. | bacterial dna was detected directly in the serum of a patient with endocarditis by broad-range 16s rrna pcr followed by sequencing and analysis of the results by the blast search. using these methods, cardiobacterium hominis was identified in 2 days from the date of serum collection. the microorganism was also isolated and identified using conventional methods (bacterial culture and biochemical tests) 17 days from the date of sample collection. this is the first report showing the direct detecti ... | 2006 | 16455944 |
| [kingella kingae]. | | 2006 | 16462965 |
| analysis of 525 samples to determine the usefulness of pcr amplification and sequencing of the 16s rrna gene for diagnosis of bone and joint infections. | the 16s rrna gene pcr in the diagnosis of bone and joint infections has not been systematically tested. five hundred twenty-five bone and joint samples collected from 525 patients were cultured and submitted to 16s rrna gene pcr detection of bacteria in parallel. the amplicons with mixed sequences were also cloned. when discordant results were observed, culture and pcr were performed once again. bacteria were detected in 139 of 525 samples. culture and 16s rrna gene pcr yielded identical documen ... | 2006 | 16517890 |
| leukotoxin confers beta-hemolytic activity to actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans is the etiologic agent of localized aggressive periodontitis, a rapidly progressing oral disease that occurs in adolescents. a. actinomycetemcomitans can also cause systemic disease, including infective endocarditis. in early work on a. actinomycetemcomitans workers concluded that this bacterium is not beta-hemolytic. more recent reports have suggested that a. actinomycetemcomitans does have the potential to be beta-hemolytic. while growing a. actinomycetemco ... | 2006 | 16552030 |
| update on the management of skin, soft-tissue, and osteoarticular infections in children. | to provide an update on the diagnosis and management of skin, soft-tissue, and osteoarticular infections in children. | 2006 | 16721144 |
| outbreak of kingella kingae skeletal system infections in children in daycare. | the objective of this study is to describe the investigation of an outbreak of one culture-proven and two presumptive cases of kingella kingae osteomyelitis detected within a 15-day period in a daycare center in israel. | 2006 | 16732151 |
| septic arthritis in western and sub-saharan african children - a review. | this article reviews what is known about the incidence, aetiology, presentation, bacteriology and management of septic arthritis in children. it compares where possible the different presentations and characteristics of this condition in the western and sub-saharan african regions. | 2007 | 16741731 |
| septic arthritis in western and sub-saharan african children - a review. | this article reviews what is known about the incidence, aetiology, presentation, bacteriology and management of septic arthritis in children. it compares where possible the different presentations and characteristics of this condition in the western and sub-saharan african regions. | 2007 | 16741731 |
| kingella kingae infections in children. | kingella kingae is a beta-hemolytic gram-negative bacillus. it was first described in the 1960's by eo king and has been reported as a cause of osteo-articular pediatric infections since the early 1980's. we performed a retrospective review of all pediatric cases of invasive k. kingae infection between 1997 and 2002, in order to define the incidence, clinical presentation and outcome of invasive k. kingae infections in a pediatric population. during the study period, a total of 24 pediatric pati ... | 2006 | 16751740 |
| kingella kingae: an emerging pediatric pathogen. | | 2006 | 16802628 |
| kingella kingae septic arthritis with endocarditis in an adult. | kingella kingae is part of the nonpathogenic flora normally found in the oral cavity and pharynx. recent reports have established that k. kingae can cause invasive infections in pediatric patients. few cases have been described in adults, however. we report a case of k. kingae arthritis of the knee followed by endocarditis in a 59-year-old woman. physicians and microbiologists should be alert to the possibility of k. kingae infection. k. kingae is easy to detect provided its specific culture req ... | 2006 | 16807042 |
| current patterns of infective endocarditis in congenital heart disease. | to assess the changing profile of infective endocarditis in patients with congenital heart disease. | 2006 | 16818488 |
| development of a broad-range 16s rdna real-time pcr for the diagnosis of septic arthritis in children. | the broad-range pcr has been successfully developed to search for fastidious, slow-growing or uncultured bacteria, and is mostly used when an empirical antibiotic treatment has already been initiated. the technique generally involves standard pcr targeting the gene coding for 16s ribosomal rna, and includes a post-pcr visualisation step on agarose gel which is a potential source of cross-over contamination. in addition, interpretation of the presence of amplified products on gels can be difficul ... | 2007 | 16904782 |
| comparative efficacies of amoxicillin, clindamycin, and moxifloxacin in prevention of bacteremia following dental extractions. | we evaluated the efficacies of oral prophylactic treatment with amoxicillin (amx), clindamycin (cli), and moxifloxacin (mxf) in the prevention of bacteremia following dental extractions (bde). two hundred twenty-one adults who required dental extractions under general anesthesia were randomly assigned to a control group, an amx group, a cli group, and an mxf group (the individuals in the drug treatment groups received 2 g, 600 mg, and 400 mg, respectively, 1 to 2 h before anesthesia induction). ... | 2006 | 16940094 |
| brevundimonas vesicularis septic arthritis in an immunocompetent child. | septic arthritis is a rapidly destructive form of joint disease. the most common causative agents in children are staphylococcus aureus and kingella kingae, followed by group a streptococcus and streptococcus pneumoniae, and in neonates, enterobacteracea and group b streptococcus. in this paper, we describe a previously healthy toddler with septic arthritis of the shoulder joint caused by brevundimonas vesicularis. prompt treatment with cefuroxime resulted in a full recovery. this is the first r ... | 2007 | 16941131 |
| cardiobacterium hominis endocarditis: two cases and a review of the literature. | cardiobacterium hominis, a member of the hacek group (haemophilus parainfluenzae, haemophilus aphrophilus, and haemophilus paraphrophilus, actinobacillus actinomycetemcomitans, c. hominis, eikenella corrodens, and kingella species), is a rare cause of endocarditis. there are 61 reported cases of c. hominis infective endocarditis in the english-language literature, 15 of which involved prosthetic valve endocarditis. there is one reported case of c. hominis after upper endoscopy and none reported ... | 2006 | 16955250 |
| [kingella kingae ostemyelitis and septic arthritis in paediatric patients. six cases from the department of pediatrics, national university hospital of iceland.] | kingella kingae (k. kingae) is a gram negative rod most often associated with septic arthritis and osteomyelitis in children. infections caused by k. kingae had not been reported in iceland when six cases were diagnosed at the pediatric department at the national university hospital of iceland. in this report we describe those cases and review the literature. | 2000 | 17018942 |
| kingella kingae spondylodiscitis in a child. | most osteoarticular infections in children are due to staphylococcus aureus. in this case, the isolation of kingella kingae by image guided disc aspiration resulted in modification and optimization of treatment. we take a look at a case of spondylodiscitis in a young child and review some of the current literature with regards to kingella kingae infections. | 2006 | 17065283 |
| identification and characterization of an rtx toxin in the emerging pathogen kingella kingae. | kingella kingae is an emerging bacterial pathogen that is increasingly recognized as the causative agent of a variety of pediatric diseases, including septic arthritis and osteomyelitis. the pathogenesis of k. kingae disease is believed to begin with colonization of the upper respiratory tract. in the present study, we examined interactions between k. kingae and cultured respiratory epithelial cells and observed potent cytotoxicity, detected by both microscopy and lactic acid dehydrogenase (ldh) ... | 2007 | 17098895 |
| pantoea agglomerans, a plant pathogen causing human disease. | we present 53 pediatric cases of pantoea agglomerans infections cultured from normally sterile sites in patients seen at a children's hospital over 6 years. isolates included 23 from the bloodstream, 14 from abscesses, 10 from joints/bones, 4 from the urinary tract, and 1 each from the peritoneum and the thorax. p. agglomerans was most associated with penetrating trauma by vegetative material and catheter-related bacteremia. | 2007 | 17442803 |
| specific real-time polymerase chain reaction places kingella kingae as the most common cause of osteoarticular infections in young children. | the use of universal 16s rdna polymerase chain reaction (pcr) has recently shown that the place of kingella kingae in osteoarticular infections (oai) in young children has been underestimated, but this technique is not the most sensitive or the most rapid method for molecular diagnosis. we developed a specific real-time pcr method to detect k. kingae dna and applied it to the etiologic diagnosis of oai. | 2007 | 17468645 |
| [a tenacious lameness in a 2-year-old child]. | spondylodiscitis, a septic infection of the intervertebral disc spondylodiscitis, a septic infection of the intervertebral disc and adjacent vertebrae, is an unusual infection, mainly affecting children and elderly people. it is classically associated with tuberculosis, but other germs such as staphylococcus aureus, streptococcus pyogenes or mitis, and some even more unususal ones (e.g. kingella kingae), are often encountered in our countries. non tuberculous spondylodiscitis is found in approxi ... | 2007 | 17566390 |
| [meningitis due to kingella kingae]. | | 2007 | 17583630 |
| molecular diagnosis of kingella kingae pericarditis by amplification and sequencing of the 16s rrna gene. | kingella kingae is a fastidious gram-negative bacillus that is considered an emerging pathogen in pediatric settings but remains less common in adults. here we describe a case of pericarditis in an immunocompetent adult host. the microorganism was identified directly from the clinical sample by molecular techniques, i.e., 16s rrna gene amplification and sequencing. | 2007 | 17634294 |
| bacterial peritonitis caused by kingella kingae. | kingella kingae is a commensal of the upper respiratory tract that occasionally causes skeletal infections in children and endocarditis in children and adults. we report a case of a 55-year-old man with liver disease and tense ascites who performed a paracentesis on himself and developed k. kingae peritonitis and bacteremia. | 2007 | 17634309 |
| pediatric pelvic osteomyelitis. | pelvic osteomyelitis is unusual in children. we retrospectively reviewed charts of patients with this infection seen at our institution. from 1998 to 2005, 31 patients with pelvic osteomyelitis were identified: 19 males and 12 females with an age range of 1.5 months to 17 years 9 months. duration of illness prior to admission ranged from 1 day to 2.5 months. chief complaints included nonspecific pain, fever, limp, and decreased weight bearing. microorganisms isolated included staphylococcus aure ... | 2007 | 17641126 |
| bacterial endocarditis due to eikenella corrodens: a case report. | of all the causes of bacterial endocarditis, hacek group consisting of haemophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae are rare causative agents. we report a case of bacterial endocarditis by e. corrodens, which is one of the members of the hacek group. | 2003 | 17643001 |
| [epidemiology and bacteriological diagnosis of paediatric acute osteoarticular infections]. | acute paediatric osteo-articular infections require a fast and sensitive diagnosis allowing a treatment directed to the causative pathogen. many micro-organisms can be incriminated, but staphylococcus aureus and kingella kingae markedly prevail. k. kingae became the first bacterial species responsible for septic arthritis in children < 3 years. more rarely, (2)haemolytic streptococci and streptococcus pneumoniae are found. the incidence of community acquired s. aureus resistant to oxacillin in o ... | 2007 | 17956825 |
| cardiobacterium hominis endocarditis: a case report and review of the literature. | the present case report describes the clinical course of a patient who presented with cardiobacterium hominis endocarditis. a review of the literature follows the case presentation. c hominis, a fastidious gram-negative bacillus, is a member of the hacek group of microorganisms (haemophilus species, actinobacillus actinomycetemcomitans, c hominis, eikenella corrodens and kingella kingae). endocarditis caused by c hominis is uncommon and generally follows a subacute course. patients may present w ... | 2005 | 18159562 |
| infective endocarditis due to haemophilus aphrophilus: a case report. | to report the case of a child with infective endocarditis caused by haemophilus aphrophilus. | 2008 | 18213436 |
| staphylococcus aureus bacteremia complicating herpes simplex virus type 1 stomatitis: case report and review of the literature. | gingivostomatitis is a common clinical manifestation of primary herpes simplex virus type 1 (hsv-1) infection in children. the most common complication of herpetic gingivostomatitis is dehydration; rarely, it may be complicated by secondary bacteremia, and kingella kingae and group a streptococcus infections have been reported to be responsible for such episodes. | 2008 | 18251654 |
| [kingella kingae: specific growth conditions in blood culture bottles]. | | 2008 | 18358221 |
| [endocarditis due to aggregatibacter (formerly: actinobacillus) actinomycetemcomitans, a bacterium that grows in characteristic star-shaped colonies]. | a 72-year-old man, having had an artificial valve for almost 20 years now, presented with tiredness that had persisted for several weeks and reported weight loss of 5 kg. in more recent days he experienced fever and cold shivers, and an associated dry cough. bearing in mind the potential for endocarditis, blood cultures were grown. in this, we identified a small, gram-negative rod with a small, smooth, raised colony that grew slowly. we considered a micro-organism from the 'hacek group', which i ... | 2008 | 18491827 |
| direct comparison of the bd phoenix system with the microscan walkaway system for identification and antimicrobial susceptibility testing of enterobacteriaceae and nonfermentative gram-negative organisms. | the phoenix automated microbiology system (bd diagnostics, sparks, md) is designed for the rapid identification (id) and antimicrobial susceptibility testing (ast) of clinically significant human bacterial pathogens. we evaluated the performance of the phoenix instrument in comparison with that of the microscan walkaway system (dade behring, west sacramento, ca) in the id and ast of gram-negative clinical strains and challenge isolates of enterobacteriaceae (n = 150) and nonfermentative gram-neg ... | 2008 | 18495856 |
| usefulness of broad-range pcr for the diagnosis of osteoarticular infections. | conventional methods such as microbiological cultures may lack the sensitivity and specificity to establish definitive diagnosis of osteoarticular infections. herein, we review the general principles and the usefulness of broad-range pcr to improve the etiological diagnosis of osteoarticular infections. | 2008 | 18525362 |
| streptobacillus moniliformis as the causative agent in spondylodiscitis and psoas abscess after rooster scratches. | we report a case of streptobacillus moniliformis spondylodiscitis accompanied by a psoas abscess in an 80-year-old man scratched by a rooster. s. moniliformis was identified from abscess fluid by use of 16s rrna gene sequencing. after 18 weeks of antimicrobial therapy, the clinical condition of the patient improved. | 2008 | 18562588 |
| empiric antibiotic therapy for acute osteoarticular infections with suspected methicillin-resistant staphylococcus aureus or kingella. | the bacterial agents causing bone and joint infections have been changing. currently, methicillin-resistant staphylococcus aureus (mrsa) and kingella kingae are emerging pathogens. for treatment of mrsa infections, clindamycin, vancomycin, and linezolid are commonly prescribed antibiotics. kingella are sensitive to most penicillins and cephalosporins. because mrsa osteoarticular infections tend to be severe, longer periods of antibiotic treatment with more frequent monitoring of inflammatory mar ... | 2008 | 18600193 |
| osteoarticular infections in belgian children: a survey of clinical, biological, radiological and microbiological data. | the aim of this study is to report the pathogens which were found most frequently to be responsible for osteo-articular infections in infants and children in belgium, and to propose an appropriate empirical antibiotic therapy applicable before identification of the responsible pathogen. clinical presentation, imaging and blood biology are also reviewed and analysed. fifty-six cases of osteo-articular infections (acute/subacute osteomyelitis, osteo-arthritis, septic arthritis, spondylodiscitis, s ... | 2008 | 18686465 |
| [pediatric osteoarticular infections caused by kingella kingae from 1995 to 2006 at chru de tours]. | use of molecular biology shows that kingella kingae is a pathogen frequently involved in osteoarticular infections in young children. this study describes the cases of osteoarticular infections due to k. kingae which happened from 1995 to 2006 in the chru of tours. the description is based on clinical and biological features. a k. kingae specific polymerase chain reaction was performed in our laboratory in order to improve k. kingae osteoarticular infections diagnosis, and is detailed here. | 2008 | 18725349 |
| kingella kingae expresses type iv pili that mediate adherence to respiratory epithelial and synovial cells. | kingella kingae is a gram-negative bacterium that colonizes the respiratory tract and is a common cause of septic arthritis and osteomyelitis. despite the increasing frequency of k. kingae disease, little is known about the mechanism by which this organism adheres to respiratory epithelium and seeds joints and bones. previous work showed that k. kingae expresses long surface fibers that vary in surface density. in the current study, we found that these fibers are type iv pili and are necessary f ... | 2008 | 18757541 |
| trimethoprim-sulfamethoxazole for osteoarthritis caused by staphylococcus aureus or kingella kingae. | | 2008 | 18818554 |
| invasive kingella kingae infections in children: clinical and laboratory characteristics. | kingella kingae, a gram-negative coccobacillus, is being increasingly recognized as an invasive pathogen in children, causing mainly bacteremia and arthritis; however, there have been only a few studies on k kingae infections to date, mostly small-scale series. the aim of this study was to report our experience with invasive k kingae infections in children who were hospitalized at a major tertiary medical center in israel. | 2008 | 19047250 |
| molecular diagnosis of kingella kingae osteoarticular infections by specific real-time pcr assay. | kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. the bacterium is best detected by rapid inoculation in blood culture systems or by real-time pcr assays. pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as rtx, which is toxic to a variety of human cell types. the locus encoding the rtx toxin is thought to be a putative virulence factor, and is, appa ... | 2009 | 19074654 |
| infective arthritis: bacterial 23s rrna gene sequencing as a supplementary diagnostic method. | consecutively collected synovial fluids were examined for presence of bacterial dna (a 700-bp fragment of the bacterial 23s rrna gene) followed by dna sequencing of amplicons, and by conventional bacteriological methods. one or more microorganisms were identified in 22 of the 227 synovial fluids (9,7%) originating from 17 patients. sixteen of the patients had clinical signs of arthritis. for 11 patients molecular and conventional bacterial examinations were in agreement. staphylococcus aureus, s ... | 2008 | 19088916 |
| pharyngeal colonization by kingella kingae in children with invasive disease. | kingella kingae organisms isolated from the blood of 3 children with invasive infections were identical by pulsed field gel electrophoresis and random amplified polymorphic dna-polymerase chain reaction analysis to those recovered from the patients' pharynx, demonstrating the likely role of upper respiratory tract colonization in the pathogenesis of the disease caused by this bacterium. | 2009 | 19106774 |
| kingella kingae: osteoarticular infections of the sternum in children: a report of six cases. | kingella kingae is increasingly recognized as a pathogen of osteoarticular infections (oai) below the age of 2 years. it was reported that bones and joints which are rarely infected by other pathogens were frequently invaded by k. kingae. based on a series of six cases, we present the typical clinical and paraclinical manifestation of k. kingae infections of the sternum and sterno-manubrial joint. | 2008 | 19308540 |
| new real-time pcr-based method for kingella kingae dna detection: application to samples collected from 89 children with acute arthritis. | inoculation of blood culture vials with joint fluid samples has revealed the important pathogenic role of kingella kingae in pediatric arthritis. however, recent studies based on broad-range 16s ribosomal dna pcr and real-time pcr without a probe suggest that conventional methods remain suboptimal. we developed a new real-time pcr method with a probe that is highly specific for k. kingae and applied it to joint fluid samples collected from 89 children with suspected arthritis admitted to our ins ... | 2009 | 19369442 |
| diversity and site-specificity of the oral microflora in the elderly. | the purpose of the present study was to describe the bacterial diversity in the oral cavity of the elderly without root caries using bacterial microarrays, and to determine the site- and subject-specificity of bacterial colonization. samples were collected from the tongue dorsum, mucosa of the buccal fold, hard palate, supragingival plaque from sound root surfaces, and subgingival plaque from the same roots. a new 16 s rrna gene-based microarray method was used for the simultaneous detection of ... | 2009 | 19373498 |
| [usefulness of synovial fluid inoculation in blood culture bottles for diagnosing kingella kingae septic arthritis: state of the question]. | | 2009 | 19409667 |
| expression of kingella kingae type iv pili is regulated by sigma54, pils, and pilr. | kingella kingae is a member of the neisseriaceae and is being recognized increasingly as an important cause of serious disease in children. recent work has demonstrated that k. kingae expresses type iv pili that mediate adherence to respiratory epithelial and synovial cells and are selected against during invasive disease. in the current study, we examined the genome of k. kingae strain 269-492 and identified homologs of the rpon and the pils and pilr genes that are essential for pilus expressio ... | 2009 | 19465661 |
| dissemination of kingella kingae in the community and long-term persistence of invasive clones. | although kingella kingae is being increasingly recognized as an important pediatric pathogen, our current understanding of the transmission of the organism is limited. the dissemination of k. kingae in the community was studied in 2 ethnic groups living side-by-side in southern israel. | 2009 | 19593253 |
| kingella kingae prosthetic valve endocarditis complicated by a paravalvular abscess. | | 2009 | 19603602 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| effects of preincubation temperature on the detection of fastidious organisms in delayed-entry samples in the bact/alert 3d blood culture system. | this study evaluates the effect of preincubation on delayed-entry samples for fastidious organisms including the hacek group, streptococcus species, neisseria meningitidis, haemophilus species and corynebacterium species for the bact/alert 3d system (biomérieux) using the fa (aerobic) medium. bottles were inoculated with two different concentrations (0.5 mcfarland and a 1:100,000 dilution) of each organism and either loaded into the system immediately or stored at 4 degrees c, room temperature ( ... | 2009 | 19733598 |
| moraxella lacunata septic arthritis in a patient with lupus nephritis. | moraxella lacunata is a rare, usually commensal gram-negative rod most commonly associated with eye infections. we report a unique case of noniatrogenic m. lacunata bacteremia and septic knee arthritis in a patient with class iii-iv lupus nephritis and speculate on the association between invasive moraxella infection and renal impairment. | 2009 | 19794049 |
| an adult case of urinary tract infection with kingella kingae: a case report. | kingella kingae, though part of the normal upper respiratory tract and genitourinary tract, is increasingly being recognized as an important human pathogen. during the past decade, it has emerged as a significant pathogen in the pediatric age group primarily causing bacteremia and osteoarticular infections. adult infection usually occurs in individuals who are severely immunocompromised and most infections have taken the form of septicemia or septic arthritis. bacteremia due to k. kingae has bee ... | 2009 | 19830146 |
| acute haemophilus parainfluenzae endocarditis: a case report. | numerous pathogens can cause infective endocarditis, including haemophilus parainfluenzae. h. parainfluenzae is part of the h. aphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae group that may cause about 3% of the total endocarditis cases, and is characterized by a subacute course and large vegetations. | 2009 | 19830211 |
| kingella kingae endocarditis and a cluster investigation among daycare attendees. | kingella kingae can cause invasive pediatric infections and outbreaks of osteomyelitis/septic arthritis in daycare facilities have been described. this is the first reported public health investigation prompted by a case of k. kingae endocarditis in an infant attending a daycare facility. a concurrent case of osteomyelitis was identified. screening of daycare contacts revealed a low rate of colonization before antibiotic prophylaxis. | 2010 | 19884874 |
| [pediatric osteo-articular infections with negative culture results: what about kingella kingae?]. | kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. the bacterium is best detected by rapid inoculation in blood culture systems or by real-time pcr assays. pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as rtx, which is toxic to a variety of human cell types. the locus encoding the rtx toxin is thought to be a putative virulence factor, and is, appa ... | 2009 | 19994673 |
| [isolated bacteraemia caused by kingella kingae]. | | 2010 | 20006566 |
| crystal structure analysis reveals pseudomonas pily1 as an essential calcium-dependent regulator of bacterial surface motility. | several bacterial pathogens require the "twitching" motility produced by filamentous type iv pili (t4p) to establish and maintain human infections. two cytoplasmic atpases function as an oscillatory motor that powers twitching motility via cycles of pilus extension and retraction. the regulation of this motor, however, has remained a mystery. we present the 2.1 a resolution crystal structure of the pseudomonas aeruginosa pilus-biogenesis factor pily1, and identify a single site on this protein r ... | 2010 | 20080557 |
| crystal structure analysis reveals pseudomonas pily1 as an essential calcium-dependent regulator of bacterial surface motility. | several bacterial pathogens require the "twitching" motility produced by filamentous type iv pili (t4p) to establish and maintain human infections. two cytoplasmic atpases function as an oscillatory motor that powers twitching motility via cycles of pilus extension and retraction. the regulation of this motor, however, has remained a mystery. we present the 2.1 a resolution crystal structure of the pseudomonas aeruginosa pilus-biogenesis factor pily1, and identify a single site on this protein r ... | 2010 | 20080557 |
| management of acute hematogenous osteomyelitis in children. | in children, osteomyelitis is primarily hematogenous in origin and acute in nature. the principal cause of osteomyelitis in children is staphylococcus aureus, and both the epidemiology and pathogenesis of s. aureus infections, including osteomyelitis, have changed in recent years owing to the emergence of community-associated methicillin-resistant s. aureus. this review focuses on advances in the diagnosis and overall management of acute hematogenous osteomyelitis in children with these changes ... | 2010 | 20109047 |
| examination of type iv pilus expression and pilus-associated phenotypes in kingella kingae clinical isolates. | kingella kingae is a gram-negative bacterium that is being recognized increasingly as a cause of septic arthritis and osteomyelitis in young children. previous work established that k. kingae expresses type iv pili that mediate adherence to respiratory epithelial and synovial cells. pila1 is the major pilus subunit in k. kingae type iv pili and is essential for pilus assembly. to develop a better understanding of the role of k. kingae type iv pili during colonization and invasive disease, we exa ... | 2010 | 20145101 |
| invasive pediatric kingella kingae infections: a nationwide collaborative study. | kingella kingae is a gram-negative coccobacillus, increasingly recognized as an invasive pediatric pathogen. to date, only few small series of invasive k. kingae infections have been published, mostly from single medical centers. a nationwide multicenter study was performed to investigate the epidemiologic, clinical, and laboratory features of children with culture-proven k. kingae infections. | 2010 | 20182400 |
| filifactor alocis--involvement in periodontal biofilms. | bacteria in periodontal pockets develop complex sessile communities that attach to the tooth surface. these highly dynamic microfloral environments challenge both clinicians and researchers alike. the exploration of structural organisation and bacterial interactions within these biofilms is critically important for a thorough understanding of periodontal disease. in recent years, filifactor alocis, a fastidious, gram-positive, obligately anaerobic rod was repeatedly identified in periodontal les ... | 2010 | 20193074 |
| rapid differentiation of francisella species and subspecies by fluorescent in situ hybridization targeting the 23s rrna. | francisella (f.) tularensis is the causative agent of tularemia. due to its low infectious dose, ease of dissemination and high case fatality rate, f. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. microbiological diagnosis is cumbersome and time-consuming. methods for the direct detection of the pathogen (immunofluorescence, pcr) have been developed but are restricted to reference laboratories. | 2010 | 20205957 |
| kingella kingae osteoarticular infections in young children: clinical features and contribution of a new specific real-time pcr assay to the diagnosis. | kingella kingae is an emerging pathogen that may be recognized as the most common bacteria responsible for osteoarticular infections (oai) in young children. however, its diagnosis remains a challenge and thus little evoked in infants, because k. kingae is a difficult germ to isolate on solid medium, and clinical signs are often mild. the main objective of this prospective study is to describe the clinical, biologic, and radiologic features of children with oai caused by k. kingae. in addition, ... | 2010 | 20357599 |
| subtle bacterial endocarditis due to kingella kingae in an infant: a case report. | a 9-month-old infant presented with fever, dyspnoea, and a murmur. echocardiography showed a mitral vegetation with significant regurgitation. mitral valve plasty was performed on day 6, and was polymerase chain reaction positive for kingella kingae. the cardiac outcome was favourable. this case illustrates a subtle presentation of k. kingae mitral valve infective endocarditis in a normal-cardaic infant, treated with early surgery, and the agent belonged to the hacek (haemophilus spp actinobacil ... | 2010 | 20465860 |
| [sacroiliitis due to kingella kingae in an adult: updates on this pathogen]. | we report here the case of a sacro-iliitis due to kingella kingae in a 35 year-old patient. this case report points out the pathogenic potency in adults of this organism which is known as a cause of invasive infections in young children. inoculation of blood culture vials with clinical specimens and the use of nucleic acid amplification have recently improved the sensitivity to identify kingella kingae and to diagnose infections which need an efficient antimicrobial therapy. | 2010 | 20478779 |
| evaluation of the analytical performance of the xpert mtb/rif assay. | we performed the first studies of analytic sensitivity, analytic specificity, and dynamic range for the new xpert mtb/rif assay, a nucleic acid amplification-based diagnostic system that detects mycobacterium tuberculosis and rifampin (rif) resistance in under 2 h. the sensitivity of the assay was tested with 79 phylogenetically and geographically diverse m. tuberculosis isolates, including 42 drug-susceptible isolates and 37 rif-resistant isolates containing 13 different rpob mutations or mutat ... | 2010 | 20504986 |
| first report of septic arthritis caused by klebsiella oxytoca. | klebsiella oxytoca is known to be a pathogen in immunodeficient adults and children. here we report the first case of a k. oxytoca infection associated with spontaneous arthritis of the knee in a child with no history of immunosuppressive therapy or previous bacterial infections. despite an initial antibiotic treatment failure, a second treatment led to a cure of the infection with no joint sequelae. | 2010 | 20573877 |
| clinical disease caused by klebsiella in 2 unrelated patients with interleukin 12 receptor beta1 deficiency. | patients with interleukin 12 (il-12)p40 or il-12 receptor β1 (il12rβ1) deficiencies are prone to develop infections caused by mycobacteria and salmonella; other infections have only been rarely observed. in this report we describe 2 unrelated patients with complete autosomal recessive il12rβ1 deficiency who suffered from sepsis attributable to klebsiella pneumoniae. a mexican boy suffered from disseminated bacille calmette-guérin disease and infections caused by k pneumoniae and candida albicans ... | 2010 | 20855390 |
| infections of people with complement deficiencies and patients who have undergone splenectomy. | the complement system comprises several fluid-phase and membrane-associated proteins. under physiological conditions, activation of the fluid-phase components of complement is maintained under tight control and complement activation occurs primarily on surfaces recognized as "nonself" in an attempt to minimize damage to bystander host cells. membrane complement components act to limit complement activation on host cells or to facilitate uptake of antigens or microbes "tagged" with complement fra ... | 2010 | 20930072 |
| [kingella kingae spondilodiscitis in an adult]. | | 2010 | 21093187 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16s rrna gene sequencing for identification of difficult-to-identify bacterial strains. | conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16s rrna gene sequencing. maldi-tof ms might represent an alternative to 16s rrna gene sequencing. | 2010 | 21106794 |
| bacterial endosymbiont localization in hyalesthes obsoletus, the insect vector of bois noir in vitis vinifera. | one emerging disease of grapevine in europe is bois noir (bn), a phytoplasmosis caused by "candidatus phytoplasma solani" and spread in vineyards by the planthopper hyalesthes obsoletus (hemiptera: cixiidae). here we present the first full characterization of the bacterial community of this important disease vector collected from bn-contaminated areas in piedmont, italy. length heterogeneity pcr and denaturing gradient gel electrophoresis analysis targeting the 16s rrna gene revealed the presenc ... | 2010 | 21183640 |
| bacterial endosymbiont localization in hyalesthes obsoletus, the insect vector of bois noir in vitis vinifera. | one emerging disease of grapevine in europe is bois noir (bn), a phytoplasmosis caused by "candidatus phytoplasma solani" and spread in vineyards by the planthopper hyalesthes obsoletus (hemiptera: cixiidae). here we present the first full characterization of the bacterial community of this important disease vector collected from bn-contaminated areas in piedmont, italy. length heterogeneity pcr and denaturing gradient gel electrophoresis analysis targeting the 16s rrna gene revealed the presenc ... | 2010 | 21183640 |
| diagnosis of e. coli tricuspid valve endocarditis: a case report. | escherichia coli is a rare cause of bacterial endocarditis. we report a case of an elderly man with cirrhosis who developed bacterial endocarditis due to e.coli. we review the literature that summarizes a total of 41 cases, with a predominance of elderly women, diabetics, and patients with urinary tract infections and prosthetic heart valves. e.coli bacteremia may be mistakenly attributed to a more benign source such as urosepsis and overlooked as an indication of endocarditis. a delay in diagno ... | 2010 | 21225584 |
| identification of hacek clinical isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry is a rapid and accurate tool for the identification of many microorganisms. we assessed this technology for the identification of 103 haemophilus parainfluenzae, aggregatibacter aphrophilus, aggregatibacter actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae (hacek) clinical isolates and 20 haemophilus influenzae clinical isolates. ninety-three percent of hacek organisms ... | 2011 | 21227988 |
| the rtxa toxin gene of kingella kingae: a pertinent target for molecular diagnosis of osteoarticular infections. | kingella kingae is an emerging osteoarticular pathogen in young children. its isolation by traditional culture methods remains difficult, underscoring the need to implement other diagnostic methods for its detection and identification, such as nucleic acid amplification tests. although the genome of this bacterium has not yet been sequenced, a toxin named rtx has been identified. the goal of this study was to develop sensitive, specific, and rapid molecular methods based on the rtxa toxin gene s ... | 2011 | 21248099 |
| [pathognomonic presentation of kingella kingae infection]. | kingella kingae is an important and probably under-recognized pathogen in children. it has a predilection for bones, joints and vascular endothelium, but can also cause bacteraemia alone. we present a case of kingella kingae that manifested itself by presternal soft tissue infection, and suggest that infection of the lower sternum and the junction between the manubrium and the xyphoid process are typical, if not pathognomonic for the organism. | 2011 | 21276404 |
| [kingella kingae endocarditis]. | | 2011 | 21288787 |
| first report of performance of the versant ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae. | we evaluated the analytical, work flow, and clinical performance of the versant ct/gc dna 1.0 assay (versant ct/gc assay, where "ct" represents chlamydia trachomatis and "gc" represents neisseria gonorrhoeae). the assay simultaneously detects chlamydia trachomatis and neisseria gonorrhoeae in swab and first-catch urine (fcu) specimens. the limit of detection (lod) was determined to be 342 copies/ml for c. trachomatis and 137 copies/ml for gc. the versant ct/gc assay detected 15 c. trachomatis se ... | 2011 | 21307209 |
| kingella kingae: an emerging pathogen in young children. | kingella kingae is being recognized increasingly as a common etiology of pediatric osteoarticular infections, bacteremia, and endocarditis, which reflects improved culture methods and use of nucleic acid-amplification techniques in clinical microbiology laboratories. k kingae colonizes the posterior pharynx of young children and is transmitted from child to child through close personal contact. day care attendance increases the risk for colonization and transmission, and clusters of k kingae inf ... | 2011 | 21321033 |
| fastidious gram-negatives: identification by the vitek 2 neisseria-haemophilus card and by partial 16s rrna gene sequencing analysis. | taxonomy and identification of fastidious gram negatives are evolving and challenging. we compared identifications achieved with the vitek 2 neisseria-haemophilus (nh) card and partial 16s rrna gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic characterization using 100 fastidious gram negative bacteria. seventy-five strains represented 21 of the 26 taxa included in the vitek 2 nh database and 25 strains represented related species not included in th ... | 2010 | 21347215 |
| microbiological profile of haematogenous osteoarticular infections in children. | introduction: staphylococcus aureus, kingella kingae and ß-hemolytic streptococcus are presently the most frequently identified bacteria in child haematogenous osteoarticular infection. objective: to determine the microbiological profile (bacteria and antibiotic susceptibility) of osteoarticular infections in a paediatric hospital, so as to adapt treatment protocols to the ecology of the bacteria isolated. patients and methods: prospective descriptive study, including children admitted for acute ... | 2011 | 21371961 |
| cytotoxic effects of kingella kingae outer membrane vesicles on human cells. | kingella kingae is an emerging pathogen causing osteoarticular infections in pediatric patients. electron microscopy of k. kingae clinical isolates revealed the heterogeneously-sized membranous structures blebbing from the outer membrane that were classified as outer membrane vesicles (omvs). omvs purified from the secreted fraction of a septic arthritis k. kingae isolate were characterized. among several major proteins, k. kingae omvs contained virulence factors rtxa toxin and pilc2 pilus adhes ... | 2011 | 21443941 |
| kingella kingae spondylodiscitis in young children: toward a new approach for bacteriological investigations? a preliminary report. | as the result of improved bacteriological techniques, kingella kingae is a slow-growing gram-negative coccobacillus that is emerging as an important cause of spondylodiscitis in children younger than 3 years of age. the high pharyngeal carrier rates of this slow-growing gram-negative coccobacillus combined with the low incidence of identified k. kingae infections is possibly explained by a low virulence of this bacterium. the use of specific real-time polymerase chain reaction (pcr) on blood sam ... | 2010 | 21455474 |
| [kingella kingae infections in the pediatric age]. | kingella kingae infections have aroused great interest in the last few years because of the increasing number of identified cases. although considered an emerging pathogen, the increase in diagnosis of these infections can probably be explained by better knowledge of the bacteria, improved microbiological diagnostic techniques and greater awareness among clinicians. k. kingae is an aerobic cocobacillus with high tropism for osteoarticular tissue, endocardium, and vascular space. this pathogen ma ... | 2011 | 21458708 |
| differentiating osteoarticular infections caused by kingella kingae from those due to typical pathogens in young children. | osteoarticular infections caused by kingella kingae are characterized by mild-to-moderate clinical and biologic inflammatory signs that are different from those caused by gram-positive cocci. a combined score was built to find the best model to predict k. kingae osteoarticular infections by using the following 4 variables: body temperature <38°c, serum c-reactive protein <55 mg/l, white blood cell count <14,000/mm, and band forms <150/mm. | 2011 | 21494171 |
| comparison of clinical and biologic features of kingella kingae and staphylococcus aureus arthritis at initial evaluation. | we conducted a retrospective study comparing the presenting clinical and biologic features of 64 children who had septic arthritis caused by kingella kingae with 26 children who had septic arthritis caused by staphylococcus aureus. children with k. kingae septic arthritis were significantly younger than those with s. aureus septic arthritis. otherwise, there were no significant differences between the 2 groups with respect to fever, location, white blood cell count, synovial fluid cell count, c- ... | 2011 | 21552181 |
| broad spectrum biofilm inhibition by kingella kingae exopolysaccharide. | cell-free extracts prepared from kingella kingae colony biofilms were found to inhibit biofilm formation by aggregatibacter actinomycetemcomitans, klebsiella pneumoniae, staphylococcus aureus, s. epidermidis, candida albicans and k. kingae. the extracts evidently inhibited biofilm formation by modifying the physicochemical properties of the cell surface, the biofilm matrix, and the substrate. chemical and biochemical analyses indicated that the biofilm inhibition activity in the k. kingae extrac ... | 2011 | 21602333 |
| the utility of matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) following introduction for routine laboratory bacterial identification. | maldi-tof ms was evaluated prospectively in a diagnostic laboratory. 927 organisms were tested in triplicate; 2351/2781 (85%) species and 2681/2781 (96%) genus identifications were correct. known issues such as the misidentification of α-haemolytic streptococci as streptococcus pneumonia were easily corrected. identifications cost $aud0.45 per isolate and were available in minutes. maldi-tof ms is rapid, accurate and inexpensive. | 2011 | 21632894 |
| antibiotic treatment for acute haematogenous osteomyelitis of childhood: moving towards shorter courses and oral administration. | acute haematogenous osteomyelitis (ahom) of childhood usually affects the long bones of the lower limbs. although almost any agent may cause ahom, staphylococcus aureus is the most common bacterium, followed by streptococcus pneumoniae and, in some countries, salmonella spp. and kingella kingae. magnetic resonance imaging (mri) has improved the diagnostic accuracy of traditional radiography and scintigraphy. except for the pre-treatment diagnostic sample from bone before the institution of antib ... | 2011 | 21640559 |
| advances in the diagnosis and management of pediatric osteomyelitis. | acute hematogenous osteomyelitis is a common disease that affects previously healthy children of all age groups. despite its importance, there are limited data in the current literature to guide many aspects of the diagnosis and therapy of this infection. over the last decade, our understanding of the etiology of this infection has changed, with increased recognition of kingella kingae and the dramatic increase in community-associated methicillin-resistant staphylococcus aureus (ca-mrsa) infecti ... | 2011 | 21789499 |
| [antibiotic therapy of bone and joint infections in children: recent changes]. | management of bone and joint infections in children associates early appropriate antibiotic therapy against staphylococcus aureus and kingella kingae and, if necessary, surgical drainage of abscess or septic arthritis. in 2007, the paediatric infectious diseases group of the french society of paediatrics (gpip) proposed guidelines for antibiotherapy in acute and non-complicated cases, with an intravenous therapy during 4 to 7days followed by oral therapy during 3weeks. | 2011 | 21733665 |
| Epidemiology of Invasive Kingella kingae Infections in Two Distinct Pediatric Populations Cohabiting in One Geographic Area. | The annual incidence of invasive Kingella kingae younger than 4 years of age was significantly higher in westernized Jews than in indigent Bedouins living side-by-side in southern Israel (12.21/100,000 and 5.83/100,000, respectively, (P<0.05). One K. kingae clone was over-represented among isolates from Jewish children, suggesting that differences in bacterial pathogenicity may contribute to the morbidity excess detected in this population group. | 2011 | 22146739 |