| pregnancy and perinatal outcomes associated with acinetobacter baumannii infection. | objective to determine perinatal and pregnancy outcomes of acinetobacter baumannii infection using clinicopathologic material from pregnant women, neonates, and perinatal postmortem examinations with positive cultures. study design this is a retrospective record review with placental and postmortem examination. results during a 5-year period, 40 positive cultures were found. three pregnancies with positive cultures close in the peripartum period were all associated with adverse outcomes includin ... | 2013 | 23943711 |
| pcr-reverse blot hybridization assay for screening and identification of pathogens in sepsis. | rapid and accurate identification of the pathogens involved in bloodstream infections is crucial for the prompt initiation of appropriate therapy, as this can decrease morbidity and mortality rates. a pcr-reverse blot hybridization assay for sepsis, the reverse blot hybridization assay (reba) sepsis-id test, was developed; it uses pan-probes to distinguish gram-positive and -negative bacteria and fungi. in addition, the assay was designed to identify bacteria and fungi using six genus-specific a ... | 2013 | 23447637 |
| effects of high hydrostatic pressure on bacterial growth on human ossicles explanted from cholesteatoma patients. | high hydrostatic pressure (hhp) treatment can eliminate cholesteatoma cells from explanted human ossicles prior to re-insertion. we analyzed the effects of hhp treatment on the microbial flora on ossicles and on the planktonic and biofilm states of selected isolates. | 2012 | 22291908 |
| development of two real-time multiplex pcr assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections. | the frequent lack of a positive and timely microbiological diagnosis in patients with lower respiratory tract infection (lrti) is an important obstacle to antimicrobial stewardship. patients are typically prescribed broad-spectrum empirical antibiotics while microbiology results are awaited, but, because these are often slow, negative, or inconclusive, de-escalation to narrow-spectrum agents rarely occurs in clinical practice. the aim of this study was to develop and evaluate two multiplex real- ... | 2015 | 25980353 |
| evaluation of the bruker maldi biotyper for identification of fastidious gram-negative rods. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has entered clinical laboratories, facilitating identification of bacteria. here, we evaluated the maldi biotyper (bruker daltonics) for the identification of fastidious gram-negative rods (gnr). three sample preparation methods, direct colony transfer, direct transfer plus on-target formic acid preparation, and ethanol-formic acid extraction, were analyzed for 151 clinical isolates. direct colony transfe ... | 2016 | 26659214 |
| antibacterial screening of secreted compounds produced by the phase i variant of photorhabdus luminescens. | in this study, antibacterial activity of metabolites secreted by the phase i variant of photorhabdus luminescens was investigated. bioactivity of these metabolites was screened against 28 different bacterial species and strains. bacterial sensitivity was determined by a modified-version of the kirby-bauer disk diffusion susceptibility method, whereas the phase i variant's culture permeate was utilized as the "antibacterial" agent. this investigation demonstrates that 11 of the 28 bacterial speci ... | 2012 | 24293735 |
| microbiological analysis of a prospective, randomized, double-blind trial comparing moxifloxacin and clindamycin in the treatment of odontogenic infiltrates and abscesses. | the objective of this study was to identify the oral pathogens found in odontogenic infections, to determine their susceptibilities to amoxicillin-clavulanic acid (amc), clindamycin (cli), doxycycline (dox), levofloxacin (lvx), moxifloxacin (mxf), and penicillin (pen), and to search for associations between specific pathogens and types of infection. swabs from patients enrolled in a randomized, double-blind phase ii trial comparing mxf with cli for the treatment of odontogenic abscesses or infla ... | 2012 | 22354306 |
| conjunctivitis due to neisseria sicca: a case report. | we report the first case, in medline-based literature, of conjunctivitis caused by gram negative diplococcus, neisseria sicca. although it is not widely accepted as such, isolation from cultures of repeated eye swab samples suggests that n. sicca may be a pathogen in conjunctival infections. positive culture for this organism should not be readily dismissed. such conjunctivitis responded favorably to treatment with netilmicin eye drops. | 2014 | 23552355 |
| nucleophilic 1,4-additions for natural product discovery. | natural products remain an important source of drug candidates, but the difficulties inherent to traditional isolation, coupled with unacceptably high rates of compound rediscovery, limit the pace of natural product detection. here we describe a reactivity-based screening method to rapidly identify exported bacterial metabolites that contain dehydrated amino acids (i.e., carbonyl- or imine-activated alkenes), a common motif in several classes of natural products. our strategy entails the use of ... | 2014 | 24937678 |
| insights into methyltransferase specificity and bioactivity of derivatives of the antibiotic plantazolicin. | peptide antibiotics represent a class of conformationally constrained natural products of growing pharmaceutical interest. plantazolicin (pzn) is a linear, polyheterocyclic natural product with highly selective and potent activity against the anthrax-causing bacterium, bacillus anthracis. the bioactivity of pzn is contingent on dimethylation of its n-terminal arg residue by an s-adenosylmethionine-dependent methyltransferase. here, we explore the substrate tolerances of two homologous pzn methyl ... | 2015 | 25635336 |
| hexr controls glucose-responsive genes and central carbon metabolism in neisseria meningitidis. | neisseria meningitidis, an exclusively human pathogen and the leading cause of bacterial meningitis, must adapt to different host niches during human infection. n. meningitidis can utilize a restricted range of carbon sources, including lactate, glucose, and pyruvate, whose concentrations vary in host niches. microarray analysis of n. meningitidis grown in a chemically defined medium in the presence or absence of glucose allowed us to identify genes regulated by carbon source availability. most ... | 2016 | 26644430 |
| performance and verification of a real-time pcr assay targeting the gyra gene for prediction of ciprofloxacin resistance in neisseria gonorrhoeae. | in the united states, 19.2% of neisseria gonorrhoeae isolates are resistant to ciprofloxacin. we evaluated a real-time pcr assay to predict ciprofloxacin susceptibility using residual dna from the roche cobas 4800 ct/ng assay. the results of the assay were 100% concordant with agar dilution susceptibility test results for 100 clinical isolates. among 76 clinical urine and swab specimens positive for n. gonorrhoeae by the cobas assay, 71% could be genotyped. the test took 1.5 h to perform, allowi ... | 2016 | 26739156 |
| evolutionary and genomic insights into meningococcal biology. | epidemic disease caused by neisseria meningitidis, the meningococcus, has been recognized for two centuries, but remains incompletely controlled and understood. there have been dramatic reductions in serogroup a and c meningococcal disease following the introduction of protein-polysaccharide conjugate vaccines, but there is currently no comprehensive vaccine against serogroup b meningococci. genetic analyses of meningococcal populations have provided many insights into the biology, evolution and ... | 2012 | 22827308 |
| bacterial flora concurrent with helicobacter pylori in the stomach of patients with upper gastrointestinal diseases. | to investigate the non-helicobacter pylori (h. pylori) bacterial flora concurrent with h. pylori infection. | 2012 | 22468090 |
| using high throughput sequencing to explore the biodiversity in oral bacterial communities. | high throughput sequencing of 16s ribosomal rna gene amplicons is a cost-effective method for characterization of oral bacterial communities. however, before undertaking large-scale studies, it is necessary to understand the technique-associated limitations and intrinsic variability of the oral ecosystem. in this work we evaluated bias in species representation using an in vitro-assembled mock community of oral bacteria. we then characterized the bacterial communities in saliva and buccal mucosa ... | 2012 | 22520388 |
| genome sequence analyses show that neisseria oralis is the same species as 'neisseria mucosa var. heidelbergensis'. | phylogenies generated from whole genome sequence (wgs) data provide definitive means of bacterial isolate characterization for typing and taxonomy. the species status of strains recently defined with conventional taxonomic approaches as representing neisseria oralis was examined by the analysis of sequences derived from wgs data, specifically: (i) 53 neisseria ribosomal protein subunit (rps) genes (ribosomal multi-locus sequence typing, rmlst); and (ii) 246 neisseria core genes (core genome mlst ... | 2013 | 24097834 |
| bacterial diversity in oral samples of children in niger with acute noma, acute necrotizing gingivitis, and healthy controls. | noma is a gangrenous disease that leads to severe disfigurement of the face with high morbidity and mortality, but its etiology remains unknown. young children in developing countries are almost exclusively affected. the purpose of the study was to record and compare bacterial diversity in oral samples from children with or without acute noma or acute necrotizing gingivitis from a defined geographical region in niger by culture-independent molecular methods. | 2012 | 22413030 |
| predictive parameters for a diagnostic bone marrow biopsy specimen in the work-up of fever of unknown origin. | to determine the role of bone marrow biopsy (bmbx), performed in association with comprehensive blood and imaging tests, in the evaluation of patients with fever of unknown origin (fuo). | 2012 | 22226833 |
| grievous temporal and occipital injury caused by a bear attack. | bear attacks are reported from nearly every part of the world. the chance of a human encountering a bear increases as the remote bear territory diminishes. the sloth bear is one of the three species of bears found in india, which inhabits the forests of india and its neighboring countries. here we describe a teenager who came to us with a critical injury involving the face, temporal and occipital bones inflicted by a sloth bear attack. he underwent a temporal exploration, facial nerve decompress ... | 2013 | 24396623 |
| polymicrobial endocarditis in intravenous heroin and fentanyl abuse. | infective endocarditis is a life threatening condition with a high mortality rate. intravenous drug abusers (ivda) are more likely to acquire endocarditis. most of the cases of infective endocarditis are caused by a single pathogen; cases of polymicrobial endocarditis are rare and they are associated with a reported mortality rate of more than 30%. only 21 cases of n. sicca endocarditis have been described in the literature since 1918, and only 15 reported cases of endocarditis which involved ac ... | 2013 | 24551699 |
| neisseria sicca endocarditis complicated by intracranial and popliteal aneurysms in a patient with a bicuspid aortic valve. | we report a case of infective endocarditis due to neisseria sicca complicated by intracranial and popliteal aneurysms and hepatic and splenic infarcts in a patient with a bicuspid aortic valve. no predisposing factor other than poor dental condition was found. the patient fully recovered after antibiotic therapy, aortic and mitral valve replacement, endovascular occlusion of the middle-cerebral artery aneurysm, and surgical treatment of the popliteal artery aneurysm. | 2013 | 23476838 |
| iatrogenic meningitis caused by neisseria sicca/subflava after intrathecal contrast injection, australia. | we report a case of invasive neisseria sicca/subflava meningitis after a spinal injection procedure during which a face mask was not worn by the proceduralist. the report highlights the importance of awareness of, and adherence to, guidelines for protective face mask use during procedures that require sterile conditions. | 2014 | 24856004 |
| molecular assay for detection of genetic markers associated with decreased susceptibility to cephalosporins in neisseria gonorrhoeae. | the incidence of antimicrobial-resistant neisseria gonorrhoeae continues to rise in canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (naats). we developed a molecular assay for surveillance use to detect mutations in genes associated with decreased susceptibility to cephalosporins that can be applied to both culture isolates and clinical samples. real-ti ... | 2015 | 25878350 |
| fast mechanically driven daughter cell separation is widespread in actinobacteria. | dividing cells of the coccoid gram-positive bacterium staphylococcus aureus undergo extremely rapid (millisecond) daughter cell separation (dcs) driven by mechanical crack propagation, a strategy that is very distinct from the gradual, enzymatically driven cell wall remodeling process that has been well described in several rod-shaped model bacteria. to determine if other bacteria, especially those in the same phylum (firmicutes) or with similar coccoid shapes as s. aureus, might use a similar m ... | 2016 | 27578753 |
| emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea. | the new superbug neisseria gonorrhoeae has retained resistance to antimicrobials previously recommended for first-line treatment and has now demonstrated its capacity to develop resistance to the extended-spectrum cephalosporin, ceftriaxone, the last remaining option for first-line empiric treatment of gonorrhea. an era of untreatable gonorrhea may be approaching, which represents an exceedingly serious public health problem. herein, we review the evolution, origin and spread of antimicrobial re ... | 2012 | 23231489 |
| a genomic approach to bacterial taxonomy: an examination and proposed reclassification of species within the genus neisseria. | in common with other bacterial taxa, members of the genus neisseria are classified using a range of phenotypic and biochemical approaches, which are not entirely satisfactory in assigning isolates to species groups. recently, there has been increasing interest in using nucleotide sequences for bacterial typing and taxonomy, but to date, no broadly accepted alternative to conventional methods is available. here, the taxonomic relationships of 55 representative members of the genus neisseria have ... | 2012 | 22422752 |
| genetic distribution of noncapsular meningococcal group b vaccine antigens in neisseria lactamica. | the poor immunogenicity of the meningococcal serogroup b (menb) capsule has led to the development of vaccines targeting subcapsular antigens, in particular the immunodominant and diverse outer membrane porin, pora. these vaccines are largely strain specific; however, they offer limited protection against the diverse menb-associated diseases observed in many industrialized nations. to broaden the scope of its protection, the multicomponent vaccine (4cmenb) incorporates a pora-containing outer me ... | 2013 | 23803905 |
| genetic characteristics of neisseria meningitidis serogroup b strains carried by adolescents living in milan, italy: implications for vaccine efficacy. | before a protein vaccine is introduced into a country, it is essential to evaluate its potential impact and estimate its benefits and costs. the aim of this study was to determine the genetic characteristics of neisseria meningitidis b (nmb) in the pharyngeal secretions of 1375 healthy adolescents aged 13-19 y living in milan, italy, in september 2012, and the possible protection offered by the two currently available nmb protein vaccines. ninety-one subjects were nm carriers (6.6%), 29 (31.9%) ... | 2013 | 23880917 |
| antimicrobial resistance in neisseria gonorrhoeae in the 21st century: past, evolution, and future. | neisseria gonorrhoeae is evolving into a superbug with resistance to previously and currently recommended antimicrobials for treatment of gonorrhea, which is a major public health concern globally. given the global nature of gonorrhea, the high rate of usage of antimicrobials, suboptimal control and monitoring of antimicrobial resistance (amr) and treatment failures, slow update of treatment guidelines in most geographical settings, and the extraordinary capacity of the gonococci to develop and ... | 2014 | 24982323 |
| chlamydia gallinacea, not c. psittaci, is the endemic chlamydial species in chicken (gallus gallus). | to investigate the prevalence and diversity of chlamydia spp. in domestic birds in china, oral and cloacal swabs of healthy chickens, ducks, geese and pigeons were collected nationwide from live-animal markets and examined by chlamydia spp. 23 s rrna gene fret-pcr followed by high-resolution melting curve analysis and confirmatory sequencing. overall, 26.2% of the birds (602/2,300) were positive for chlamydia spp. and five chlamydia spp. were identified. while occasional detection of c. suis and ... | 2016 | 26778053 |
| [preliminary analyses on bacterial diversity and resistance in infection-related skin disorders]. | to explore the bacterial diversity and resistance in infection-related skin disorders. | 2015 | 26704163 |
| identifying neisseria species by use of the 50s ribosomal protein l6 (rplf) gene. | the comparison of 16s rrna gene sequences is widely used to differentiate bacteria; however, this gene can lack resolution among closely related but distinct members of the same genus. this is a problem in clinical situations in those genera, such as neisseria, where some species are associated with disease while others are not. here, we identified and validated an alternative genetic target common to all neisseria species which can be readily sequenced to provide an assay that rapidly and accur ... | 2014 | 24523465 |
| pharyngeal carriage of neisseria species in the african meningitis belt. | neisseria meningitidis, together with the non-pathogenic neisseria species (npns), are members of the complex microbiota of the human pharynx. this paper investigates the influence of npns on the epidemiology of meningococcal infection. | 2016 | 27018131 |
| serogroup-specific interaction of neisseria meningitidis capsular polysaccharide with host cell microtubules and effects on tubulin polymerization. | we have previously shown that during late stages of the infectious process, serogroup b meningococci (menb) are able to escape the phagosome of in vitro-infected human epithelial cells. they then multiply in the cytosolic environment and spread intracellularly and to surrounding cells by exploiting the microtubule cytoskeleton, as suggested by results of infections in the presence of microtubule inhibitors and evidence of nanotubes connecting neighboring cells. in this study, by using microtubul ... | 2014 | 24166951 |
| treatment of allergic diseases: application to clinical practice of a new concept of mutual substitutions of antibody molecules on the surface of mast cells. | | 2007 | 20525152 |
| neonatal early-onset sepsis evaluations among well-appearing infants: projected impact of changes in cdc gbs guidelines. | to determine (a) the proportion of asymptomatic infants born at ≥35 weeks gestation evaluated for early-onset sepsis (eos) and exposed to postnatal antibiotics; (b) reasons for and outcomes of the evaluations, and (c) anticipated changes when applying the centers for disease control and prevention (cdc) 2010 guidelines to this study population. | 2012 | 22814941 |
| neonatal early-onset sepsis evaluations among well-appearing infants: projected impact of changes in cdc gbs guidelines. | to determine (a) the proportion of asymptomatic infants born at ≥35 weeks gestation evaluated for early-onset sepsis (eos) and exposed to postnatal antibiotics; (b) reasons for and outcomes of the evaluations, and (c) anticipated changes when applying the centers for disease control and prevention (cdc) 2010 guidelines to this study population. | 2012 | 22814941 |
| polymicrobial infective endocarditis caused by neisseria sicca and haemophilus parainfluenzae. | infective endocarditis is a common clinical problem in industrialized countries. risk factors include abnormal cardiac valves, a history of endocarditis, intracardiac devices, prosthetic valves and intravenous drug use. we report a case of polymicrobial infective endocarditis in a 33 year-old female with a history chronic heroin use caused by neisseria sicca and haemophilus parainfluenzae. we believe the patient was exposed to these microbes by cleansing her skin with saliva prior to injection. ... | 2015 | 27051571 |
| neisseria gonorrhoeae false-positive result obtained from a pharyngeal swab by using the roche cobas 4800 ct/ng assay in new zealand in 2012. | the roche cobas 4800 ct/ng assay is a commonly used commercial system for screening for neisseria gonorrhoeae infection, and previous studies have shown the method to be highly sensitive and specific for urogenital samples. we present the first confirmed clinical n. gonorrhoeae false-positive result using the cobas 4800 ng assay, obtained from testing a pharyngeal swab sample and caused by cross-reaction with a commensal neisseria strain. | 2013 | 23486711 |
| metagenomic analysis of nitrate-reducing bacteria in the oral cavity: implications for nitric oxide homeostasis. | the microbiota of the human lower intestinal tract helps maintain healthy host physiology, for example through nutrient acquisition and bile acid recycling, but specific positive contributions of the oral microbiota to host health are not well established. nitric oxide (no) homeostasis is crucial to mammalian physiology. the recently described entero-salivary nitrate-nitrite-nitric oxide pathway has been shown to provide bioactive no from dietary nitrate sources. interestingly, this pathway is d ... | 2014 | 24670812 |
| population and functional genomics of neisseria revealed with gene-by-gene approaches. | rapid low-cost whole-genome sequencing (wgs) is revolutionizing microbiology; however, complementary advances in accessible, reproducible, and rapid analysis techniques are required to realize the potential of these data. here, investigations of the genus neisseria illustrated the gene-by-gene conceptual approach to the organization and analysis of wgs data. using the gene and its link to phenotype as a starting point, the bigsdb database, which powers the pubmlst databases, enables the assembly ... | 2016 | 27098959 |
| species-level core oral bacteriome identified by 16s rrna pyrosequencing in a healthy young arab population. | reports on the composition of oral bacteriome in arabs are lacking. in addition, the majority of previous studies on other ethnic groups have been limited by low-resolution taxonomic assignment of next-generation sequencing reads. furthermore, there has been a conflict about the existence of a 'core' bacteriome. | 2016 | 27193835 |
| multiplex strand invasion based amplification (msiba) assay for detection of chlamydia trachomatis and neisseria gonorrhoeae. | nucleic acid amplification tests have become a common method for diagnosis of stis due to their improved sensitivity over immunoassays and traditional culture-based methods. isothermal nucleic acid amplification methods offer significant advantages over polymerase chain reaction (pcr) because they do not require sophisticated instruments needed for thermal cycling of pcr. we recently reported a novel isothermal nucleic acid amplification method, strand invasion-based amplification (siba), which ... | 2016 | 26837460 |
| utility of specimens positive for neisseria gonorrhoeae by the aptima combo 2 assay for assessment of strain diversity and antibiotic resistance. | in our jurisdiction, the aptima combo 2 assay (gen-probe, inc.) is used to detect neisseria gonorrhoeae from specimens collected at clinics for sexually transmitted infections (sti) and from select community patients. in addition, swabs are also collected for n. gonorrhoeae culture, susceptibility testing, and sequence typing (st). since only a small proportion of samples from provincial cases undergo culture, the available trends in antimicrobial susceptibility and predominant strain types may ... | 2013 | 24108603 |
| molecular epidemiology of selected sexually transmitted infections. | neisseria gonorrhoeae (ng), chlamydia trachomatis (ct), trichomonas vaginalis (tv) and mycoplasma genitalium (mg) are established pathogens for human genital tract. however, the role of ureaplasma urealyticum (uu) and ureaplasma parvum (up) in genital pathology is poorly unerstood. a prospective study to investigate the prevalence of above infections was performed on a cohort of 1,718 consecutive patients attending a genitourinary medicine (gum) clinic. a previously published in-house real-time ... | 2013 | 24046809 |
| sigma factor rpon (σ54) regulates pile transcription in commensal neisseria elongata. | human-adapted neisseria includes two pathogens, neisseria gonorrhoeae and neisseria meningitidis, and at least 13 species of commensals that colonize many of the same niches as the pathogens. the type iv pilus plays an important role in the biology of pathogenic neisseria. in these species, sigma factor rpod (σ(70)), integration host factor, and repressors regf and crga regulate transcription of pile, the gene encoding the pilus structural subunit. the type iv pilus is also a strictly conserved ... | 2013 | 23899162 |
| polymicrobial infective endocarditis caused by neisseria sicca and haemophilus parainfluenzae. | infective endocarditis is a common clinical problem in industrialized countries. risk factors include abnormal cardiac valves, a history of endocarditis, intracardiac devices, prosthetic valves and intravenous drug use. we report a case of polymicrobial infective endocarditis in a 33 year-old female with a history chronic heroin use caused by neisseria sicca and haemophilus parainfluenzae. we believe the patient was exposed to these microbes by cleansing her skin with saliva prior to injection. ... | 2015 | 27051571 |
| susceptibility pattern of microorganisms isolated by percutaneous needle biopsy in nonbacteremic pyogenic vertebral osteomyelitis. | pyogenic vertebral osteomyelitis (vo) is diagnosed according to several lines of evidence: clinical, biological, radiological, and histological. definitive diagnosis requires the isolation of a causative pathogen or histological confirmation. the aim of our study was to describe the microorganisms isolated by percutaneous needle biopsy (pnb) and to analyze their susceptibility patterns, in order to assess the possibility of empirical combination therapy for the treatment of nonbacteremic patient ... | 2015 | 26438497 |
| neisseria sicca endocarditis presenting as multiple embolic brain infarcts. | a 58 year old male presented with a 14 day history of progressive forgetfulness and aggressiveness. he had a history of human immunodeficiency virus infection, ischemic cardiomyopathy, and a myxomatous mitral valve (status post saint jude's mitral valve replacement 8 years before presentation). international normalized ratio was supra-therapeatutic on admission. a non-contrast computed tomography of the brain indicated multiple infarcts with hemorrhagic conversion. the source was later found to ... | 2015 | 26284256 |
| neisseria infection of rhesus macaques as a model to study colonization, transmission, persistence, and horizontal gene transfer. | the strict tropism of many pathogens for man hampers the development of animal models that recapitulate important microbe-host interactions. we developed a rhesus macaque model for studying neisseria-host interactions using neisseria species indigenous to the animal. we report that neisseria are common inhabitants of the rhesus macaque. neisseria isolated from the rhesus macaque recolonize animals after laboratory passage, persist in the animals for at least 72 d, and are transmitted between ani ... | 2013 | 23382234 |
| structural analysis of haemoglobin binding by hpua from the neisseriaceae family. | the neisseriaceae family of bacteria causes a range of diseases including meningitis, septicaemia, gonorrhoea and endocarditis, and extracts haem from haemoglobin as an important iron source within the iron-limited environment of its human host. herein we report crystal structures of apo- and haemoglobin-bound hpua, an essential component of this haem import system. the interface involves long loops on the bacterial receptor that present hydrophobic side chains for packing against the surface of ... | 2015 | 26671256 |
| bartholinitis due to aggregatibacter aphrophilus: a case report. | aggregatibacter aphrophilus, a commensal of the oro-pharyngeal flora and member of the hacek group of organisms, is an uncommonly encountered clinical pathogen. it has already been described as the causative agent of brain abscesses, empyema, meningitis, sinusitis, otitis media, bacteriemia, pneumonia, osteomyelitis, peritonitis, endocarditis and wound infections. herein we report the first case of bartholinitis due to a. aphrophilus. | 2016 | 27756231 |
| [detection of unusual uropathogens during a period of three years in a regional hospital]. | urinary tract infections (utis) are one of the most frequent both in the community and in hospitals infectious diseases. the etiology of urinary tract infections is well established but may vary depending on various factors such as age, the presence of underlying diseases such as diabetes, instrumental procedures such as urinary catheterization or exposure to antibiotics or previous hospitalizations. utis diagnosed cases were retrospectively reviewed for unusual microorganisms over a period of 3 ... | 2015 | 25904515 |
| native-valve tricuspid endocarditis due to neisseria sicca with bilateral pulmonary embolism. | | 2013 | 24007771 |
| [spondylodiscitis associated to neisseria sicca endocarditis]. | | 2013 | 23414682 |
| fatal neisseria sicca endocarditis. | | 2013 | 23297179 |
| high-resolution and specific detection of bacteria on complex surfaces using nanoparticle probes and electron microscopy. | the study of the interaction of bacteria with surfaces requires the detection of specific bacterial groups with high spatial resolution. here, we describe a method to rapidly and efficiently add nanogold particles to oligonucleotide probes, which target bacterial ribosomal rna. these nanogold-labeled probes are then used in an in situ hybridization procedure that ensures both cellular integrity and high specificity. electron microscopy subsequently enables the visualization of specific cells wit ... | 2015 | 26018431 |
| clinical pathology of bartholin's glands: a review of the literature. | the bartholin's glands are located symmetrically at the posterior region of the vaginal opening and play an important role in the female reproductive system. these two pea-sized glands are involved in mucus secretion and vaginal lubrication. cyst formation in the glands is common and results from mucus build-up in gland ducts. it is important to monitor such cysts because they may occur in the form of carcinomas. larger cysts and abscesses are found in the lower vestibular region and typically p ... | 2015 | 26195958 |
| bacterial diversity and community structure of supragingival plaques in adults with dental health or caries revealed by 16s pyrosequencing. | dental caries has a polymicrobial etiology within the complex oral microbial ecosystem. however, the overall diversity and structure of supragingival plaque microbiota in adult dental health and caries are not well understood. here, 160 supragingival plaque samples from patients with dental health and different severities of dental caries were collected for bacterial genomic dna extraction, pyrosequencing by amplification of the 16s rdna v1-v3 hypervariable regions, and bioinformatic analysis. h ... | 2016 | 27499752 |
| subgingival microbiome in smokers and non-smokers in korean chronic periodontitis patients. | smoking is a major environmental factor associated with periodontal diseases. however, we still have a very limited understanding of the relationship between smoking and subgingival microflora in the global population. here, we investigated the composition of subgingival bacterial communities from the pooled plaque samples of smokers and non-smokers, 134 samples in each group, in korean patients with moderate chronic periodontitis using 16s rrna gene-based pyrosequencing. a total of 17,927 reads ... | 2015 | 25283067 |
| development of a novel engineered antibody targeting neisseria species. | a neissaria bacterial pilus sugar, bacillosamine, was synthesized and, for the first time, used as a probe to screen a single-chain variable fragment (scfv). | 2017 | 27888365 |
| intraspecific 16s rrna gene diversity among clinical isolates of neisseria species. | in the present work, nearly the entire 16s rrna gene sequences of 46 clinical samples of neisseria spp. were determined, and the aligned sequences were analyzed to investigate the diversity of 16s rrna genes in each commensal neisseria species. two 16s rrna types were identified in two neisseria sicca strains, three 16s rrna types in five neisseria macacae strains, fourteen 16s rrna types in twenty neisseria flavescens isolates, and fourteen 16s rrna types in nineteen neisseria mucosa isolates. ... | 2014 | 24020769 |
| genotyping of commensal neisseria spp strains by pulsed-field gel electrophoresis and 16s rrna gene sequencing. | we investigated the diversity of the primary sequences of the 16s rrna genes among 46 commensal neisseria strains and evaluated the use of this approach as a molecular typing tool in comparison with pfge analysis. | 2017 | 28374932 |