| comparison of methods for rapid immunofluroescent staining of group a streptococci and neisseria gonorrhoeae. | immunofluorescent staining reactions for group a streptococci and neisseria gonorrhoeae were equivalent to those of standard "slow" methods when slides were heated on a serological water bath at 50 c for periods one to four minutes. no false negative reactions occurred when this method was used. false negative reactions occasionally occurred when previously described rapid methods which employed a slide warmer were used. | 1977 | 72052 |
| type-14 pneumococcal vaccine for prevention of gonorrhoea. | | 1979 | 86759 |
| classes of antibodies in acute gonorrhoea. | in patients with acute gonorrhoea the antibody responses to envelope antigens of neisseria gonorrhoea were investigated with a sensitive enzyme-linked immunosorbent assay. igg antibodies predominated even early in the disease but igm and iga antibodies were present. surprisingly there were circulating antibodies linked to secretory piece. these were largely but not entirely iga and were presumed to have been absorbed from sites of local production. | 1979 | 86886 |
| prostatic enlargement associated with symptomatic and asymptomatic gonorrhoea in adolescent men: an argument for routine rectal examination. | ten adolescent men presented with prostatic enlargement and positive anterior urethral cultures for gonococci. six of them had urethritis, but four were asymptomatic; the prostate is therefore implicated as a reservoir for the asymptomatic carrier and it is strongly recommended that the rectum should be included in the routine physical examination of sexually active adolescent men. | 1977 | 72583 |
| cefotaxime for bacterial meningitis. | | 1979 | 87714 |
| pathogenicity of and beta-lactamase production by branhamella (neisseria) catarrhalis. | | 1977 | 73082 |
| studies on the influence of antigens on the results with the gonococcal complement fixation test in patients with uncomplicated and complicated gonorrhoea. | the gonococcal complement fixation test (gcft) was investigated with regard to its sensitivity and specificity by testing serum specimens from (a) female patients attending a vd out-patient clinic because of suspected gonorrhoea, (b) patients with a proven, uncomplicated, urogenital gonococcal, infection, (c) patients with disseminated gonococcal infection (dgi). three different pools of gonococcal (gc) antigens were used which were comprised of gc strains from two different geographical areas. ... | 1977 | 73333 |
| [antigenic characteristics and various biological properties of glycoprotein from neisseria meningitidis of serological group a]. | it was shown that the antigen determining the group specificity of meningococcus belonging to serological group a was of mixed polysaccharide-protein nature. carbohydrate component is responsible for the interaction with the group-specific antibodies in this antigen. glycoprotein can be isolated both from the cells and from the culture fluid where it passes during the n. meningitidis cultivation in fluid nutrient medium. the described antigen possesses no properties of endotoxin. | 1978 | 75626 |
| correlation with different immunotypes of gonococcal antigens associated with growth in vivo. | | 1978 | 75944 |
| chlamydia trachomatis urethritis in men attending a venereal disease clinic: a culture and therapeutic study. | urethral specimens from 459 male patients attending a venereal disease clinic were studied for presence of chlamydia trachomatis and neisseria gonorrhoeae. of the 459 patients, 362 had symptoms suggestive of urethritis. in these 362, gonorrhoea was diagnosed in 78 (22%), while 88 (24%) harboured c. trachomatis; 15 harboured both organisms. of those patients from whom c. trachomatis was isolated, 17% had no subjective symptoms of urethritis; the same percentage of symptomless carriers of gonococc ... | 1978 | 76400 |
| safety and immunogenicity of a neisseria meningitidis type 2 protein vaccine in animals and humans. | two neisseria meningitidis vaccines consisting principally of outer membrane protein (lot 138i-0) or outer membrane protein plus group c polysaccharide (lot 138i-m1) were prepared from the group c type 2 strain 138i. lipopolysaccharide and lipid were removed by gel filtration in the presence of sodium deoxycholate. the vaccines were found to be nontoxic and nonpyrogenic in animals. they provided active protection in mice against mucin-enhanced killing by group b type 2 meningococci and induced g ... | 1978 | 77885 |
| degradation of the polysaccharide component of gonococcal lipopolysaccharide by gonococcal and meningococcal sonic extracts. | an extract made from the supernatant of neisseria gonorrhoeae gc2 strain 1291 degraded the gc2 polysaccharide antigen. chemical analysis of this polysaccharide indicated it contains glucose, galactose, glucosamine, galactosamine, glucosamine-6-phosphate, heptose, 2-keto-3-deoxyotonate, and ethanolamine and is the polysaccharide component of gonococcal lipopolysaccharide. degradation of the polysaccharide by sonic extracts resulted either in complete loss of antigenicity and immunogenicity or in ... | 1978 | 78894 |
| is group-specific meningococcal vaccination resulting in epidemics caused by groups of virulent meningococci? | in 1976 routine vaccination against neisseria meningitidis serogroups a and c was started in the finnish armed forces. a case of fulminant, complicated pneumonia caused by group-y meningococcus in a vaccinated recruit, prompted a study of the distribution of the meningococcal groups isolated from the recruits in the same unit. 14 (46%) of the 31 isolates from 84 recruits were group y. group-y meningococcus was rarely isolated from unvaccinated controls. these results suggest that widespread vacc ... | 1978 | 79765 |
| group-specific meningococcal vaccination and epidemics caused by other groups of meningococci. | | 1978 | 80699 |
| crossed immunoelectrophoretic analysis of neisseria meningitidis antigens and of corresponding antibodies in patients with meningococcal disease. | sixty-three different antigens of neisseria meningitidis groups a, b and c were demonstrated by means of crossed immunoelectrophoresis against rabbit antiserum. the group-specific polysaccharides a and c were identified in the immunoprecipitate pattern. the majority of the remaining antigens were common to all three groups of meningococci. a clearcut rise in antibodies was found in all of 27 patients with meningococcal meningitis or septicaemia. these antibodies were directed against both group- ... | 1978 | 81590 |
| meningococcal vaccines and disease due to other serogroups. | | 1978 | 81948 |
| antigenic heterogeneity of the non-serogroup antigen structure of neisseria gonorrhoeae lipopolysaccharides. | studies of the antigenic structure of the polysaccharide component of gonococcal lipopolysaccaride (lps) indicated that the non-serogroup antigen structure is antigenically heterogeneous. immunodiffusion studies of gc4 strain 8551 indicated that in addition to the gc serogroup determinent, this polysaccharide contains two other sets of determinants, one which is shared with the other five gc serogroups and a second which is shared by the gc1 and gc3 serogroups. enzyme-linked immunosorbent assay ... | 1979 | 93575 |
| genital occurrence of oral microbiota. | recent studies indicate that tonsillar gonococcal infection or colonization is fairly common. carriage rates of about 8% have been found. these studies also indicate that oro-genital contacts are common. since very little is known about the amount of oral microbiota transmitted to the genitals, we have studied the occurrence of oral streptococci and neisseria species in urethra and cervix. among 128 patients attending an std-clinic we found 10 carriers of oral streptococci, one streptococcus mit ... | 1978 | 82332 |
| precipitating antibodies against neisseria meningitidis in normal sera and their possible origin. | crossed immunoelectrophoresis was used to study precipitating antibodies against neisseria meningitidis in sera from 214 normal persons and in human gammaglobulin. a polyspecific meningococcal antigen preparation and a corresponding rabbit antiserum constituted the reference system. all the sera contained one to five precipitins against n. meningitidis which could be identified and quantified by means of the reference system. the precipitin score, which expresses the number and titre of precipit ... | 1978 | 82371 |
| comparison of antigenic heterogeneity of neisseria gonorrhoeae strains by micro-immunofluorescence and serum bactericidal tests. | the antigenic heterogeneity of neisseria gonorrhoeae strains was assessed by the micro-immunofluorescence (micro-if) and the serum bactericidal tests. the micro-if test verified the antigenic heterogeneity of nine strains received from the center for disease control and placed them into immunotypes a and b. the serum bactericidal system also detected different antigenic determinants among the strains. although the micro-if and bactericidal assays did not correspond in each instance, the overall ... | 1978 | 83299 |
| lectins in diagnostic microbiology: use of wheat germ agglutinin for laboratory identification of neisseria gonorrhoeae. | a lectin slide agglutination test has been developed for the confirmatory identification of neisseria gonorrhoeae. with wheat germ lectin as an agglutinin, 164 of 165 clinical isolates of n. gonorrhoeae gave a 3 to 4+ reaction within 6 to 8 min. four gonococcal isolates, even though negative by the fluoresecent-antibody method, gave strong positive reactions with the wheat germ lectin. among 23 isolates of neisseria meningitidis tested, which included representatives of sero-groups a, b, c,d, x, ... | 1979 | 94598 |
| common heat stable protective antigen in gonococci. | 1. gonococci possess a common heat stable antigen that is not identical to endotoxin. 2. a model of gonococcal peritonitis is described that simulates human disease in its progression from local to disseminated infection and its milieu of mucin and hemoglobin. 3. vaccination with the heat stable antigen protected against disseminated infection and raised the ld50s from 100 to 1000-fold. 4. vaccination with antigen from one strain of gonococci conferred protection against heterologous infection. ... | 1979 | 95067 |
| comparison of counterimmunoelectrophoresis and elisa for diagnosis of bacterial meningitis. | | 1979 | 84221 |
| beta-lactamase production in commensal neisseriaceae. | | 1979 | 85221 |
| disc agar diffusion antimicrobial susceptibility tests with beta-lactamase producing neisseria gonorrhoeae. | the emergence of beta-lactamase-producing strains of neisseria gonorrhoeae has led to a reexamination of the role of the disc agar diffusion method in susceptibility testing of gonococci. our data show that the disc agar diffusion test can be used to screen for beta-lactamase production by these organisms. the disc tests were done on gc agar base supplemented with 1% isovitalex. an inoculum of 10(8) colony forming units/ml and either a 10-unit-penicillin or a 10-microgram-ampicillin disc were us ... | 1978 | 96085 |
| n-terminal amino acid sequence of pilin isolated from pseudomonas aeruginosa. | the amino-terminal amino acid sequence of the pili protein from pseudomonas aeruginosa k pili is presented. the sequence is compared with those reported by others for pilin obtained from neisseria gonorrhoeae and moraxella nonlique-faciens. all three sequences are highly homologous, contain only two hydrophilic residues in the first 22 positions, and contain an unusual amino acid, n-monomethylphenylalanine, at the amino terminus. | 1978 | 96098 |
| [makeup and properties of a polycomponent meningococcal vaccine]. | the polycomponent meningococcae vaccine represented a preparation of the high-molecular fraction of meningococcus cell wall substances. meningococcae strains for the vaccine preparation were chosen in such a way that the end preparation contained antigens of group specificity a, b, c and also other antigens detected in the cell wall of strains of epidemiological significance. protein, group polysaccharides., lipopolysaccharides and nucleic acids were included into the vaccine composition. in dos ... | 1978 | 85399 |
| comparison of counter-current immunoelectrophoresis, latex agglutination, and radioimmunoassay in detection of soluble capsular polysaccharide antigens of haemophilus influenzae type b and neisseria meningitidis of groups a or c. | three serological methods, radioimmunoassay (ria), latex agglutination (lx), and counter-current immunoelectrophoresis (ciep), for sensitivity in the detection of the capsular polysaccharide antigen of haemophilus influenzae type b or neisseria meningitidis groups a and c were compared. ria was consistently the most sensitive, lx the next, and ciep the least sensitive. when ria and lx were used to test cerebrospinal fluid (csf) samples of patients with meningitis, they gave very similar results. ... | 1978 | 85636 |
| serology of neisseria gonorrhoeae. demonstration of strain-specific antigens by immunoelectrophoresis, immunofluorescence and co-agglutination techniques. | among a group of four selected strains of neisseria gonorrhaeae, common and strain-specific antigens were demonstrated by immunoelectrophoresis, indirect immunofluorescence (ifl) and co-agglutination (coa). in preparations of ultrasonically-disrupted organisms, the strain-specific antigens appeared in crossed-line immunoelectrophoresis (c-lie) with antigen containing intermediate agar gel as a two-peak precipitin line, one peak close to the antigen well, the other towards the anode. a similar pa ... | 1979 | 86276 |
| counterimmunoelectrophoresis for rapid identification of blood-culture isolates. | a total of 303 blood cultures that were positive by examination of gram-stained smears were tested immediately by counterimmunoelectrophoresis for detection of bacterial antigens. antigen was detected in all 82 blood cultures containing streptococcus pneumoniae and 11 of 22 with klebsiella pneumoniae, two of two with haemophilus influenzae, and one of one with neisseria meningiditis. false-positive cross-reactions in 265 tests occurred only with pneumococcal omniserum in two cases of nongroupabl ... | 1979 | 86294 |
| [electron microscopic representation of the pili structure of neisseria gonorrhoeae (author's transl)]. | the technique of negative staining and ultra-thin section has been used for investigations of 30 neisseria gonorrhoeae strains in order to represent the structure of pili (fimbriae) electron microscopically. the staining of the gonococci was effected by phosphotungstic acid (0,5%). the pili ascertained were 30 to 60 a thick. in course of in vitro passages up to 10. subculture morphological changes of the pili have been observed. the application of trisbuffer or solution of hylase (hyaluronidase) ... | 1979 | 86464 |
| [identification of species of the family neisseriaceae by means of counterimmunoelectrophoresis inhibition]. | | 1979 | 86640 |
| [standardization of the inoculation conditions and the principles for assessing the suitability of solid nutrient media for the meningococcus]. | | 1979 | 86641 |
| [bactericidal antibody micro-assay for detection of antibodies against meningococci (author's transl)]. | the best way to detect protective antibodies against meningococci in human serum seems to prove its bactericidal activity. for this a bactericidal antibody assay was described by who. for this test, however, a high amount of baby rabbit-serum is required. therefore a modification was introduced by reducing the test volumes, performing the test in microtiterplates and using ttc (triphenyl tetracolium chloride), as a growth indicator that changes from colourless to purple red in the presence of gr ... | 1978 | 96630 |
| [hyaluronidase in meningococcus]. | a total of 204 meningococcus strains were tested for the presence of hyaluronidase, and 45.5% of the strains were found to contain it. strains penetrating into the cerebrospinal fluid were the ones which largely produced the enzyme (in 83% of the cases). the enzyme was revealed only in 25.5% of the strains habituating on the nasopharyngeal mucosa. hyaluronidase was mostly found in the meningococcus strains referred to the serological group a; strains of other serological groups and ungrouped str ... | 1978 | 96634 |
| [use of trypsinized meningococci to obtain highly specific sera]. | | 1978 | 96636 |
| an electron microscopic india ink technique for demonstrating capsules on microorganisms: studies with streptococcus pneumoniae, staphylococcus aureus, and neisseria gonorrhoeae. | a technique using india ink in electron microscopic preparations was used to study bacterial capsules. capsules were demonstrated on streptococcus pneumoniae and staphylococcus aureus (strain m and the smith diffuse variant) from in vitro cultures. two types of false capsules were observed on neisseria gonorrhoeae grown in vitro or obtained from human urethral exudates. no true capsules were definitively identified on gonococci from these sources. the technique described does not require the use ... | 1979 | 92512 |
| [improved method of hemosensitin preparation for the production of meningococcal group-specific diagnostic agents]. | | 1979 | 92604 |
| the value of smears in the diagnosis of gonorrhoea in the female. | of 332 consecutive patients attending the female venereal disease clinic, auckland hospital, 74 patients had gonorrhoea confirmed by culture, but gram stained slides were positive in only 36 of these patients. five other patients had positive gram stained smears but were culture negative. the study showed the value of gram stained smear in the rapid diagnosis of gonorrhoea in the female. | 1979 | 93256 |
| practical considerations in using counterimmunoelectrophoresis to identify the principal causative agents of bacterial meningitis. | many clinical laboratories are currently using counterimmunoelectrophoresis (cie) as an aid in the rapid diagnosis of bacterial meningitis. because cross-reactions among causative agents have been reported, the present study was undertaken to explore the problems that might occur when reference and commercial antisera are used in cie. broth cultures of 35 bacterial strains were tested with 76 reference and commercial antisera by cie. some of the antisera tested failed to react with their homolog ... | 1979 | 93606 |
| relative opsonic and protective activities of antibodies against k1, o and lipid a antigens of escherichia coli. | the k1 escherichia coli capsular antigen has been implicated as a virulence factor because of the frequency of isolation of k1 containing strains from certain invasive human infections. in the study of the interaction between k1 strains, normal human polymorphonuclear cells (pmns) and fresh human serum, we have found varying susceptibility to phagocytosis and killing; thus, the in vitro opsonophagocytic and in vivo protective role of k1, somatic o and core glycolipid antibodies remain unclear. w ... | 1979 | 93778 |
| [transport medium for the meningococcus]. | | 1979 | 94133 |
| bacterial sensitivity to minocycline (minocin) of clinical isolations. | | 1977 | 95945 |
| prevention of autolysis in suspensions of neisseria gonorrhoeae by mercuric ions. | mercuric ions were the only metal ions which prevented autolysis of neisseria gonorrhoeae, as measured both by stabilization of optical density and by prevention of release of [3h]diaminopimelic acid. | 1978 | 97273 |
| meningitis caused by neisseria meningitidis type w-135. | | 1978 | 97374 |
| effect of different preparation procedures on the ultrastructure of gonococci. | culture samples of neisseria gonorrhoeae and escherichia coli were studied by transmission and electron microscopy to evaluate the effect of different preparation procedures on the pilation of these organisms. unfixed as well as fixed bacteria showed only few, long, filamentous appendages when investigated in ultrathin sections, negatively stained specimens, or critical-point dried preparations. snap-frozen specimens of e. coli showed many short and thin pili after being shadowed with carbon and ... | 1979 | 87241 |
| [isolation of penicillinase forming gonococci in munich]. | | 1978 | 97520 |
| immunological and serological diversity of neisseria gonorrhoeae: identification of new immunotypes and highly protective strains. | gonococci, irrespective of serotype or immunotype, varied significantly in their capacity to induce immunity in animal models, and in vitro serological relatedness did not always insure in vivo cross-protection. by using a serum bactericidal assay followed by in vivo cross-protection studies, new immunotypic strains which were highly protective were identified from cultures isolated in different geographical areas and from patients with various clinical manifestations. beta-lactamase production ... | 1979 | 88414 |
| lipopolysaccharide-derived serotype polysaccharides from neisseria meningitidis group b. | three immunologically distinct types of polysaccharides have been isolated by diethylaminoethyl (deae) chromatography from the lipopolysaccharide extracts of group b neisseria meningitidis. all types contain a set of common determinants, as well as distinct ones; all of these determinants are detectable by either immunodiffusion or enzyme-linked immunosorbent assay (elisa). the polysaccharides elute from a sepharose 4b column in the range of 2-3 x 10(5) daltons and have isoelectric points from 4 ... | 1979 | 88491 |
| [simple method of obtaining erythrocytic diagnostic agents for titration of antimeningococcal antibodies]. | | 1979 | 89237 |
| [immunoprophylaxis problem in meningococcal infection]. | | 1979 | 89767 |
| [study of molecular heterogeneity and chemical nature of polymeric components of the meningococcus cell wall]. | the high-molecular fraction of substances of the cell wall of meningococci, groups a and b, isolated in free volume in gel filtration through sepharose 4b and containing both group and intergroup antigens proved to be consisting of 2 subfractions in gel-filtration through bio-gel a-150m. molecular weight of the first was within the range of 100--150 million dalton, and of the second--of 3 to 100 million dalton. in dissociation in sodium deoxycholate the high molecular fraction complex compound o ... | 1978 | 97883 |
| characterization of fractions containing the thermostable agglutinogens of neisseria gonorrhoeae. | extract was prepared by heat treatment (100 degrees c. 2 h) of bacteria of n. gonorrhoeae strain 8551. the acid precipitate (ph 2.5) of the extract was resolved by gel chromatography (sepharose cl-6b) into material eluted with the void volume (fr. i) and material retarded by the column (fr. ii). fr. i contained at least three precipitinogens, the antigenic determinant a of lps and the protein determinant b. the antigens demonstrable in fr. i were also detected in an outer membrane preparation ob ... | 1979 | 89783 |
| antigenic subunit of the polypeptide antigenic complex of the melvin strain of neisseria gonorrhoeae. | | 1979 | 90508 |
| production of beta-bactamase by a strain of neisseria gonorrhoeae when cultured in presence of ethidium bromide. | | 1979 | 90931 |
| more than one antigen contributes to the immunogenicity of neisseria gonorrhoeae in the guinea pig chamber model. | gonococci adapted to growth in guinea pig chambers [strain bs4 (agar)] were predominantly smooth organisms and produced a type-specific antigen. a vaccine prepared by treating these gonococci with formalin, protected guinea pig chambers against homologous challenge in contrast to a similarly treated laboratory strain (bsdh) which had been selected in vitro from the same parent strain and which did not produce the type-specific antigen. surface washes of bs4 (agar) contained the type-specific ant ... | 1979 | 91661 |
| differential binding of penicillin by membrane fractions from penicillin-susceptible and -resistant gonococci. | an inverse relationship between binding of [(14)c]penicillin to cells of neisseria gonorrhoeae and the minimal inhibitory concentration of penicillin was recently reported by us. herein we report a similar relationship in binding by cytoplasmic membranes derived from 10 strains of gonococci. membranes were extracted after braun homogenization and differential centrifugation of the resulting extracts. membranes derived from penicillin-susceptible cells bound 140 to 200 nmol of [(14)c]penicillin p ... | 1978 | 96727 |
| contribution of a tem-1-like beta-lactamase to penicillin resistance in neisseria gonorrhoeae. | two beta-lactamase-producing strains of neisseria gonorrhoeae were studied. the substrate profile, molecular weight, and isoelectric point of their beta-lactamases were similar to those of the tem-1 enzyme produced by many gram-negative bacilli. the gonococcal beta-lactamase was cell bound during exponential growth and was most likely located in the periplasm. penicillin hydrolysis was efficient in intact cells, suggesting that the cell-bound beta-lactamase was freely accessible to benzylpenicil ... | 1978 | 96728 |
| sk&f 75073, new parenteral broad-spectrum cephalosporin with high and prolonged serum levels. | sk&f 75073, a new parenteral cephalosporin, was found to have broad in vitro and in vivo antibacterial activity including isolates usually resistant to cephalothin and cefazolin. this activity included indole-positive proteus and enterobacter species and some serratia isolates. proteus mirabilis strains were particularly susceptible, as were haemophilus influenzae and neisseria species. the activity of sk&f 75073 against gram-positive bacteria was poorer than that of the control cephalosporins. ... | 1978 | 96734 |
| bacteria induce lymphokine synthesis polyclonally in human b lymphocytes. | we have studied the ability of various bacteria to stimulate human lymphocytes to produce leukocyte migration inhibitory factor (lif). mononuclear cells from adult and cord blood as well as purified t and b lymphocytes were stimulated with killed bacteria. the culture supernatants were tested for the presence of lif by the agarose migration method. all nine bacterial strains tested activated unseparated mononuclear cells and b lymphocytes but not t cells to produce lif. lif was also present in c ... | 1978 | 98583 |
| role of iron in disseminated gonococcal infections. | strains of neisseria gonorrhoeae isolated from disseminated gonococcal infections have enhanced ability to acquire iron in an experimental host when compared with urogenital gonococcal isolates from patients with uncomplicated infections. | 1978 | 97231 |
| quantitative measurement of phagocytosis of neisseria gonorrhoeae by mouse peritoneal macrophages. | the simultaneous labeling of gonococci with [6-3h]uracil and of mouse peritoneal macrophages with l-[u-14c]leucine permits a quantitative assessment of the association of gonococci with macrophages under various experimental conditions. colony-type t1 (piliated) gonococci associated more than t4 (nonpiliated) organisms at 4 degrees c, but at 37 degrees c the association of t4 gonococci with macrophages exceeded that for the t1 organisms. the association of t1 gonococci with macrophages could be ... | 1978 | 97235 |
| identification of a new sequence-specific endonuclease, ngoii, from neisseria gonorrhoeae. | a class ii restriction endonuclease which recognizes the same nucleotide sequence as endor.haeiii has been found in four of seven isolates of neisseria gonorrhoeae tested. | 1978 | 97270 |
| perihepatitis associated with salpingitis in adolescents. | perihepatitis, one manifestation of complicated gonorrhea, is infrequently diagnosed and reported. this study was undertaken to examine the incidence of gonococcal perihepatitis in adolescents as well as to study the effect, if any, of this condition on tests of liver function. accordingly, the records of 137 adolescents with salpingitis were evaluated. right upper quadrant tenderness or hepatic enlargement was noted in 27, while amino transferase level elevations were observed in 19 of the 59 i ... | 1978 | 98646 |
| gonorrhea detection in posthysterectomy patients. | a retrospective study of women examined at the venereal disease clinic after hysterectomy was made to determine which culture site was most likely to show anogenital neisseria gonorrhoeae infection when the cervix was absent. seventeen of 104 such patients were culture positive for n gonorrhoeae. rates of positivity for our culture sites were as follows: urethral, 17/17 (100%); vaginal, 7/17 (41%); and rectal, 2/17 (12%). | 1978 | 98647 |
| [nonpilar surface appendages in gonococci]. | | 1978 | 95982 |
| the bacterial pathogenesis of acute pelvic inflammatory disease. | to study the bacterial pathogenesis of acute pelvic inflammatory disease, peritoneal fluid was obtained by culdocentesis in 133 of 344 women with this disease. in 104 of the specimens bacteria were identified both in the gram-stained smear and culture. neisseria gonorrhoeae was isolated from the lower genital tract in over half of these women, and there were 3 patterns of bacterial recovery from peritoneal fluid: n gonorrhoeae alone (22%), n gonorrhoeae and other organisms (32%), and nongonococc ... | 1978 | 98745 |
| serogroup identification of neisseria meningitidis: comparison of an antiserum agar method with bacterial slide agglutination. | a serum agar method for serogrouping neisseria meningitidis is described and compared with conventional bacterial slide agglutination. there was 93% agreement for 300 strains examined individually by each method. among strains from serogroups a, b, c, y, and w135, there was 100% correlation, whereas strains from serogroup 29e (z') had only 67% correlation. the serum agar method was rapid, as well as easy to perform and interpret. the potential benefits of this method for epidemiological studies ... | 1978 | 96123 |
| the activity of cephalosporins on beta-lactamase-producing neisseria gonorrhoeae. | the antibacterial activity of cephaloridine, cephalothin, cephalexin, cephradine, cefazolin, cefamandole, cefuroxime and cefoxitin was determined for six beta-lactamase-producing gonococci isolated in great britain and the usa. cefuroxime was most active against small and large inocula, then cefoxitin, while cephaloridine was least active. cefamandole was more active than cefazolin and cephalothin, but only on small inocula, and these three antibiotics, with the slightly inferior cephalexin and ... | 1978 | 98833 |
| identification of some basic extractable compounds produced by neisseria gonorrhoeae and neisseria meningitidis in a defined medium. | electron capture gas-liquid chromatography and mass spectrometry have been used to identify some of the basic extractable heptafluorobutyric anhydride reactive compounds found in a defined medium after 20 h of growth by neisseria gonorrhoeae and n. meningitidis. acetoin, 2,3-butanediol, pyrroline, and 1,3-diaminopropane were identified by both gas chromatography and mass spectrometry; 2-hydroxy-pyrrolidine and 3-aminomethyl-pyrrolidine were tentatively identified by mass spectrometry. a possible ... | 1978 | 96124 |
| comparison of a radiometric procedure with conventional methods for identification of neisseria. | a radiometric procedure was compared with the conventional cystine tryptic agar (cta) sugar fermentation method for identification of neisseria species. four different atcc cultures of neisseria were identified by both procedures with identical results. the only difference noted was that the radiometric procedure required 3 h for completion, whereas the conventional cta sugar method required overnight incubation. the radiometric procedure was also compared with the fluorescent antibody (fa) and ... | 1978 | 96125 |
| identification and grouping of neisseria meningitidis directly on agar plates by coagglutination with specific antibody-coated protein a-containing staphylococci. | it has been shown that neisseria meningitidis can be grouped by coagglutination directly upon growth on sheep blood or chocolate agar plates. all positive reactions were group specific, and only a single colony was required for a positive reaction. there was variation seen in the effectiveness of commercial antisera in preparing sensitive reagents. certain throat and sputum isolates of group z organisms failed to react by coagglutination, although they would react directly with antiserum in whol ... | 1978 | 96128 |
| [bacteriological diagnosis of gonorrhea]. | | 1978 | 99333 |
| the susceptibility of neisseria gonorrhoeae to cefoxitin sodium. | | 1978 | 99417 |
| a comparison of the in vitro activity of rosamicin, erythromycin, spiramycin, penicillin and tetracycline against n. gonorrhoeae, including beta-lactamase producing isolates. | | 1978 | 99424 |
| outer membrane protein antigens in an enzyme-linked immunosorbent assay for salmonella enteric fever and meningococcal meningitis. | outer membrane protein preparations were obtained from strains of salmonella and neisseria meningitidis. solubilized cell envelope (ce) fractions from s. typhi and salmonella groups a, b, c, and e had very similar electrophoretic mobilities on polyacrylamide gel, and common antigens were demonstrated by immunodiffusion. ce appeared to be a more satisfactory antigen than the more purified preparation (t/tei) in the enzyme-linked immunosorbent assay (elisa) with sera from typhoid and paratyphoid p ... | 1978 | 99452 |
| attachment role of gonococcal pili. optimum conditions and quantitation of adherence of isolated pili to human cells in vitro. | gonoccocal pili facilitate attachment of virulent neisseria gonorrhoeae to human cells. to characterize this attachment function, purified gonococcal pili isolated from four strains possessing antigenically distinct pili were radiolabeled with 125i and used to measure the attachment of pili to various human cells in vitro. human buccal and cervical-vaginal mucosal epithealial cells, fallopian tube mucosa, and sperm bound pili in greater numbers per micrometer2 of surface area (1--10) than fetal ... | 1978 | 96134 |
| sympathetic joint effusion in septic arthritis. | | 1978 | 99527 |
| identification of neisseria gonorrhoeae by carbohydrate disc reactions on a modified fermentation medium. | the identification of neisseria gonorrhoeae by the use of a carbohydrate disc reaction on a modified fermentation medium is described. 70 out of the 71 strains tested produced detectable acid in 18--24 hours from the dextrose disc and no acid from maltose, sucrose or lactose discs. one strain required 48 hours for good growth and acid production from dextrose disc only. the carbohydrate disc method was compared with a standard fermentation method using sugars incorporated in serum-free medium, 6 ... | 1978 | 96274 |
| [local, natural or artificial immunity, and its importance in the protection against meningococcal disease]. | | 1978 | 99792 |
| [meningococcus carriers among university hospital personnel]. | | 1977 | 99793 |
| [types of gonococci]. | | 1978 | 99901 |
| [electron-microscopic study of the effect of biseptol on the gonococcus in vitro and in vivo]. | | 1978 | 99902 |
| [study of biological properties of lyophilized gonococcal strains for the purpose of their use for vaccine production. 1]. | | 1978 | 99903 |
| [methacycline in the therapy of gonorrhea in men]. | | 1978 | 99904 |
| [antibiotic concentration in the serum of women with gonorrhea administered benzylpenicillin and bicillin-3]. | | 1978 | 99905 |
| [allergization of the body by neisseria meningitidis. i. the problem of the occurrence of n. meningitidis in cases of bronchial asthma of bacterial etiology (author's transl)]. | the object of the authors work was the examination of nasopharyngeal swabs on the prevalence of neisseria meningitidis. a group of 222 patients suffering from asthma bronchiale of bacteriological etiology was under observation in order to be able to prepare an autovaccine. nasopharyngeal swabs were inoculated on special nutrient media. neisseria meningitidis was found in 22%, mainly from nasopharyngeal swabs, together with haemophilus sp. and streptococcus pneumoniae. in 59% of examined cases th ... | 1978 | 99912 |
| [fluorescent antibody technique in identification of neisseria gonorrhoeae-microcolonies grown on membrane filters (author's transl)]. | filter membranes (nucleopore, no. n060, cpr 01300, pore size 0.6 mu) are inoculated with a pure suspension of n. gonorrhoeae or a urethral smear from patients suffering from gonococcal urethritis. the membranes are placed on chocolate agar and incubated for three hours. after fixation in formaldehyd, grown microcolonies of n. gonorrhoea are detected microscopically after incubation of the membranes with a fluorescein-isothiocyanate labeled gonococcal antiserum (difco, charge no. 605907) (fig. 3- ... | 1978 | 99913 |
| [antagonistic and bacteriocinogenic activity of meningococci]. | antagonistic and bacteriocinogenic activity was studied in 169 strains of meningococci of various serological groups and with different localization in the human organism at the time of isolation. bacteriocinogenic activity was revealed in all the meningococcus strains (by delayed antagonism method), and, in addition, antagonistic activity was found in 100 strains. the inhibitory activity was the greatest in meningococci isolated from the cerebrospinal fluid. the data obtained suggest an importa ... | 1978 | 99921 |
| [the appearance of penicillin resistant gonococci. clinical, genetic and epidemiologic problems]. | | 1978 | 96408 |
| activity of azlocillin and mezlocillin against gram-negative organisms: comparison with other penicillins. | the activities of azlocillin and mezlocillin were compared with those of carbenicillin, ticarcillin, and pirbenicillin against a wide range of gram-negative organisms. the two new drugs were considerably more active than carbenicillin against klebsiella species and escherichia coli. carbenicillin was twice as active against proteus mirabilis as mezlocillin and four times as active as azlocillin. against pseudomonas aeruginosa, azlocillin was eight times as active as carbenicillin. azlocillin and ... | 1978 | 96726 |
| [attempts at use of indirect hemagglutination in the diagnosis of gonorrhea]. | | 1978 | 98361 |
| sensitivity of gonococcus strains isolated in rotterdam to epicillin, ampicillin, penicillin and tetracycline. | quantitative determinations of the sensitivity to epicillin, ampicillin, penicillin and tetracycline were carried out for 67 gonococcus strains isolated from 67 males with urogenital gonorrhoea, and 38 strains isolated from 38 females with urogenital/rectal gonorrhoea. the results of the sensitivity determinations for ampicillin, penicillin and tetracycline were compared with the findings of a previous investigation. it was found that the gonococcus strains isolated from males had become slightl ... | 1978 | 98362 |
| quantitative determination of the serum antibody response to the capsular polysaccharide of bacteroides fragilis subspecies fragilis in women with pelvic inflammatory disease. | the antibody response to the capsular polysaccharide of bacteroides fragilis subspecies fragilis in women with acute pelvic inflammatory disease was quantitated using a sensitive radioactive antigen-binding assay. antibody detected by this technique correlated highly with antibody measured by quantitative precipitin analysis (r = 0.99). paired acute- and convalescent-phase sera obtained from women from whom b. fragilis subspecies fragilis was isolated by culdocentesis showed significantly greate ... | 1978 | 98597 |
| phenotypic and epidemiologic correlates of auxotype in neisseria gonorrhoeae. | previous studies from seattle, wash., suggested that strains of neisseria gonorrhoeae which require arginine, hypoxanthine, and uracil (arg-hyx-ura- auxotype) are unifomly highly susceptible to penicillin g, are relatively resistant to complement-dependent killing by heated, pooled human serum, and are associated with disseminated gonococcal infection. for further study of the epidemiology of these strains and for analysis of the susceptibility to penicillin, serum sensitivity, and the nutrition ... | 1978 | 98600 |
| serum bactericidal action and activation of the classic and alternate complement pathways by neisseria gonorrhoeae. | in order to more fully understand the host defense mechanisms against gonococcal infections, we decided to define the role of the classic and alternate complement pathways in gonococcal ba. sera and infecting isolates were collected from several patients with genital and disseminated gonococcal infections. sera from two never-infected subjects and a hypogammaglobulinemic patient were also collected. sera from patients with genital gonorrhea and never-infected controls demonstrated marked ba for ... | 1978 | 98602 |
| technique for transcervical intrauterine inoculation of the mouse. | suspensions of india ink or gonococci were inoculated into the murine uterus through the cervix with a simple plastic vaginal speculum and a 23 gauge blunted needle. the inoculated suspensions were distributed throughout both uterine horns, cervix, and vagina, but did not flow into the peritoneal cavity. this method avoided surgical stress as well as being simple, fast, and reproducible. | 1978 | 98665 |
| neonatal gonococcal orogastric contamination. | | 1978 | 97404 |
| microbiology of the vagina in children: normal and potentially pathogenic organisms. | vaginal cultures from 100 healthy girls, 2 months to 15 years of age, were examined for the presence of normal and potentially pathogenic microorganisms. corynebacterium vaginale, yeast species, and genital mycoplasmas were isolated from vaginal cultures from 13.5 %, 28%, and 28% of the girls examined, respectively. colonization with these organisms was not associated with signs or symptoms of vaginitis. neisseria gonorrhoeae was isolated from a 4-year-old with purulent vaginitis. trichomonas va ... | 1978 | 98750 |
| asymptomatic gonococcal urethritis in selected males. | | 1978 | 99054 |