| vaccination with live escherichia coli expressing brucella abortus cu/zn superoxide dismutase protects mice against virulent b. abortus. | vaccination of mice with escherichia coli expressing brucella cu/zn superoxide dismutase (sod) [e. coli(pbssod)] induced a significant level of protection against virulent brucella abortus challenge, although this level was not as high as the one reached with b. abortus vaccine strain rb51. in addition, vaccination with e. coli(pbssod) induced antibodies to cu/zn sod and a strong proliferative response of splenocytes when stimulated in vitro with a thioredoxin-cu/zn sod fusion protein. | 1999 | 9916121 |
| cloning and expression of the dnak gene of campylobacter jejuni and antigenicity of heat shock protein 70. | campylobacter jejuni is a leading cause of infectious diarrhea throughout the world. in addition, there is growing evidence that guillain-barré syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by c. jejuni infection. in the present study, the hrca-grpe-dnak gene cluster of c. jejuni was cloned and sequenced. the dnak gene consists of an open reading frame of 1,869 bp and encodes a protein with a high degree of homology to other bacterial 70 ... | 1999 | 10024560 |
| genomic fingerprinting and development of a dendrogram for brucella spp. isolated from seals, porpoises, and dolphins. | genomic dna from reference strains and biovars of the genus brucella was analyzed using pulsed-field gel electrophoresis (pfge). fingerprints were compared to estimate genetic relatedness among the strains and to obtain information on evolutionary relationships. electrophoresis of dna digested with the restriction endonuclease xbai produced fragment profiles for the reference type strains that distinguished these strains to the level of species. included in this study were strains isolated from ... | 1999 | 10098687 |
| effect of early antibiotic treatment on the antibody response to cytoplasmic proteins of brucella melitensis in mice. | to test whether antibiotic therapy hampers the antibody response to brucella antigens, 30 balb/c mice were infected with brucella melitensis h38 and randomized for treatment with doxycycline administered intraperitoneally for 42 days starting at 7 or 28 days postinfection (p.i.) (groups dox7 and dox28, respectively) or for no treatment (control group). antibodies to smooth lipopolysaccharide (lps) reached peak levels (mean optical density [od] = 2.618) between days 56 and 70 p.i. in the control ... | 1999 | 10225853 |
| molecular characterization of a brucella species large dna fragment deleted in brucella abortus strains: evidence for a locus involved in the synthesis of a polysaccharide. | a brucella melitensis 16m dna fragment of 17,119 bp, which contains a large region deleted in b. abortus strains and dna flanking one side of the deletion, has been characterized. in addition to the previously identified omp31 gene, 14 hypothetical genes have been identified in the b. melitensis fragment, most of them showing homology to genes involved in the synthesis of a polysaccharide. considering that 10 of the 15 genes are missing in b. abortus and that all the polysaccharides described in ... | 1999 | 10338472 |
| cross-reactivity between the rheumatoid arthritis-associated motif eqkraa and structurally related sequences found in proteus mirabilis. | cross-reactivity or molecular mimicry may be one of the underlying mechanisms involved in the etiopathogenesis of rheumatoid arthritis (ra). antiserum against the ra susceptibility sequence eqkraa was shown to bind to a similar peptide esrral present in the hemolysin of the gram-negative bacterium proteus mirabilis, and an anti-esrral serum reacted with eqkraa. there was no reactivity with either anti-eqkraa or anti-esrral to a peptide containing the ederaa sequence which is present in hla-drb1* ... | 1999 | 10338479 |
| experimental brucella ovis infection in pregnant ewes. | forty yearling brucella-free ewes were inoculated with brucella ovis by the conjunctival route in mid or late first pregnancy. only a few ewes excreted b ovis during pregnancy and gave birth to stillborn lambs, but most of them excreted the organism at lambing or during lactation. one of the 11 lambs which were born alive but died before they were weaned was found to be infected postmortem. in contrast, none of the 46 surviving lambs which were reared in isolation until adulthood, was found to b ... | 1999 | 10371013 |
| brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family rhizobiaceae. | brucellae have been reported to be phylogenetically related to bacteria of the family rhizobiaceae. in the present study, we used a panel of monoclonal antibodies (mabs) to brucella outer membrane proteins (omps) to determine the presence of common omp epitopes in some representative bacteria of this family, i.e., ochrobactrum anthropi, phyllobacterium rubiacearum, rhizobium leguminosarum, and agrobacterium tumefaciens, and also in bacteria reported to serologically cross-react with brucella, i. ... | 1999 | 10391877 |
| genetic characterization of a tn5-disrupted glycosyltransferase gene homolog in brucella abortus and its effect on lipopolysaccharide composition and virulence. | we constructed a rough mutant of brucella abortus 2308 by transposon (tn5) mutagenesis. neither whole cells nor extracted lipopolysaccharide (lps) from this mutant, designated ra1, reacted with a brucella o-side-chain-specific monoclonal antibody (mab), bru-38, indicating the absence of o-side-chain synthesis. compositional analyses of lps from strain ra1 showed reduced levels of quinovosamine and mannose relative to the levels in the parental, wild-type strain, 2308. we isolated dna flanking th ... | 1999 | 10417145 |
| epididymitis by brucella ovis: experimental infection in virgin ram lambs. | ten sexually immature rams were experimentally infected with brucella ovis, to verify the antibody kinetics and its localization in urinary and genital tracts. all animals became positive to the complement fixation test from the 2nd post infection (p.i.) week and reached the maximum titre (1:256) on the 4th p.i. week. bacteriemia was demonstrated on 3rd, 4th and 5th p.i. weeks. two animals, respectively slaughtered 11 and 13 weeks after the infection, showed macroscopic and microscopic genital l ... | 1999 | 10423741 |
| outer membrane proteins omp10, omp16, and omp19 of brucella spp. are lipoproteins. | the deduced sequences of the omp10, omp16, and omp19 outer membrane proteins of brucella spp. contain a potential bacterial lipoprotein processing sequence. after extraction with triton x-114, these three proteins partitioned into the detergent phase. processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. the three proteins were radioimmunoprecipitated from [(3)h]palmitic acid-labeled brucella abortus lysates with monoclonal antibodies. ... | 1999 | 10456959 |
| identification of an is711 element interrupting the wboa gene of brucella abortus vaccine strain rb51 and a pcr assay to distinguish strain rb51 from other brucella species and strains. | brucella abortus vaccine strain rb51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. the genetic mutations that are responsible for the roughness and the attenuation of strain rb51 have not been identified until now. also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain rb51 from its parent strain 2308 is available. in the present study, we demonstrate that the wboa gene encoding a glycosyltransferas ... | 1999 | 10473532 |
| improvement of the immune response to foot and mouth disease virus vaccine in calves by using avridine as adjuvant. | the epidemiological analysis of the cattle population during the eradication plan of foot and mouth disease (fmd) in argentina clearly indicated a higher incidence of the disease in animals within their first year of age. it is important to improve the efficacy of the vaccination in those animals. in a previous report, we have shown the effect of an immunomodulator, avridine (avr), in the enhancement of the immune response elicited by fmd virus (fmdv) vaccines in experimental hosts [berinstein, ... | 1999 | 10490231 |
| [isolation of brucella ovis from semen of sheep seropositive by elisa and with clinically normal genitals]. | | 1999 | 10509410 |
| protection of mice against brucellosis by vaccination with brucella melitensis wr201(16mdeltapurek). | human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. a vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. vaccines currently used in animals are unsuitable for human use. we tested a live, attenuated, purine-auxotrophic mutant strain of brucella melitensis ... | 1999 | 10531243 |
| the outer membrane of brucella ovis shows increased permeability to hydrophobic probes and is more susceptible to cationic peptides than are the outer membranes of mutant rough brucella abortus strains. | the permeability of the outer membrane (om) to hydrophobic probes and its susceptibility to bactericidal cationic peptides were investigated for natural rough brucella ovis and for mutant rough brucella abortus strains. the om of b. ovis displayed an abrupt and faster kinetic profile than rough b. abortus during the uptake of the hydrophobic probe n-phenyl-naphthylamine. b. ovis was more sensitive than rough b. abortus to the action of cationic peptides. bactenecins 5 and 7 induced morphological ... | 1999 | 10531286 |
| complement fixation test to assess humoral immunity in cattle and sheep vaccinated with brucella abortus rb51. | the live attenuated brucella abortus strain rb51 is a rifampin-resistant, lipopolysaccharide (lps) o-chain-deficient mutant of virulent b. abortus 2308. the reduced o-chain content in rb51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to lps. the absence of available serologic tests for rb51 also complicates the diagnosis of possible rb51 infections in humans exposed to this strain. ... | 1999 | 10548564 |
| characterization of specific immune responses of mice inoculated with recombinant vaccinia virus expressing an 18-kilodalton outer membrane protein of brucella abortus. | using the shuttle vector pmco2 and the vaccinia virus wild-type wr strain, we constructed a recombinant virus expressing an 18-kda outer membrane protein of brucella abortus. balb/c mice inoculated with this virus produced 18-kda protein-specific antibodies, mostly of immunoglobulin g2a isotype, and in vitro stimulation of splenocytes from these mice with purified maltose binding protein-18-kda protein fusion resulted in lymphocyte proliferation and gamma interferon production. however, these mi ... | 2000 | 10618289 |
| identification of brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis. | bacteria of the genus brucella are facultative intracellular pathogens which have developed the capacity to survive and multiply in professional and nonprofessional phagocytes. the genetic basis of this aspect of brucella virulence is still poorly understood. to identify new virulence factors, we have adapted signature-tagged transposon mutagenesis, which has been used essentially in animal models, to an in vitro human macrophage infection model. a library of 1,152 brucella suis 1330 tagged mini ... | 2000 | 10678941 |
| molecular characterization of brucella strains isolated from marine mammals. | recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as brucella species by conventional phenotypic analysis. this study found the 16s rrna gene from one representative isolate was identical to the homologous sequences of brucella abortus, b. melitensis, b. canis, and b. suis. is711-based dna fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical brucella species. in general, fingerprint patterns grouped by ... | 2000 | 10699036 |
| characterization of heat, oxidative, and acid stress responses in brucella melitensis. | brucella melitensis is a facultative intracellular pathogen which is able to survive and replicate within phagocytic cells. therefore, it has to adapt to a range of different hostile environments. in order to understand the mechanisms of intracellular survival employed by virulent b. melitensis 16m, an initial approach consisting of analysis of the differences in patterns of protein synthesis in response to heat, oxidative, and acid ph stresses by two-dimensional (2-d) polyacrylamide gel electro ... | 2000 | 10768994 |
| brucella abortus and its closest phylogenetic relative, ochrobactrum spp., differ in outer membrane permeability and cationic peptide resistance. | the outer membrane (om) of the intracellular parasite brucella abortus is permeable to hydrophobic probes and resistant to destabilization by polycationic peptides and edta. the significance of these unusual properties was investigated in a comparative study with the opportunistic pathogens of the genus ochrobactrum, the closest known brucella relative. ochrobactrum spp. oms were impermeable to hydrophobic probes and sensitive to polymyxin b but resistant to edta. these properties were traced to ... | 2000 | 10816465 |
| overexpression of protective antigen as a novel approach to enhance vaccine efficacy of brucella abortus strain rb51. | brucella abortus strain rb51 is an attenuated rough strain that is currently being used as the official live vaccine for bovine brucellosis in the united states and several other countries. we reasoned that overexpression of a protective antigen(s) of b. abortus in strain rb51 should enhance its vaccine efficacy. to test this hypothesis, we overexpressed cu/zn superoxide dismutase (sod) protein of b. abortus in strain rb51. this was accomplished by transforming strain rb51 with a broad-host-rang ... | 2000 | 10816475 |
| complementation of brucella abortus rb51 with a functional wboa gene results in o-antigen synthesis and enhanced vaccine efficacy but no change in rough phenotype and attenuation. | brucella abortus rb51 is a stable rough, attenuated mutant vaccine strain derived from the virulent strain 2308. recently, we demonstrated that the wboa gene in rb51 is disrupted by an is711 element (r. vemulapalli, j. r. mcquiston, g. g. schurig, n. srirauganathan, s. m. halling, and s. m. boyle, clin. diagn. lab. immunol. 6:760-764, 1999). disruption of the wboa gene in smooth, virulent b. abortus, brucella melitensis, and brucella suis results in rough, attenuated mutants which fail to produc ... | 2000 | 10858205 |
| identification of protective outer membrane antigens of brucella ovis by passive immunization of mice with monoclonal antibodies. | outer membrane proteins (omps) and rough lipopolysaccharide (r-lps), the main surface antigens of brucella ovis, display surface-exposed epitopes. mixtures of monoclonal antibodies (mabs) to both antigens were previously shown to protect mice against a b. ovis challenge. to further identify the antigens involved, seven mabs against brucella omps (omp10, omp16, omp19, omp25, omp31, omp2b and omp1) and three to r-lps were tested for protection either individually or in combinations. significant re ... | 2000 | 10865193 |
| a multiplex approach to molecular detection of brucella abortus and/or mycobacterium bovis infection in cattle. | a multiplex amplification and detection platform for the diagnosis of mycobacterium bovis and brucella abortus infection simultaneously in bovine milk and nasal secretions was developed. this system (designated the bovine pathogen detection assay [bpda]-pcr) consists of duplex amplification of species-specific targets (a region of the bcsp31k gene of b. abortus and a repeat-sequence region in the hsp65 gene of m. bovis, respectively). this is followed by a solid-phase probe capture hybridization ... | 2000 | 10878051 |
| fluorescent whole-cell hybridization with 16s rrna-targeted oligonucleotide probes to identify brucella spp. by flow cytometry. | a whole-cell hybridization assay with fluorescent oligonucleotide probes derived from the 16s rrna sequence of brucella abortus in combination with flow cytometry has been developed. with the three fluorescent probes selected, a positive signal was observed with all the representative strains of the species and biovars of brucella and with a total of nine different brucella clinical isolates. using the b9 probe in the hybridization assay, it was possible to discriminate between brucella suis bio ... | 2000 | 10878084 |
| validation of the abbreviated brucella amos pcr as a rapid screening method for differentiation of brucella abortus field strain isolates and the vaccine strains, 19 and rb51. | the brucella amos pcr assay was previously developed to identify and differentiate specific brucella species. in this study, an abbreviated brucella amos pcr test was evaluated to determine its accuracy in differentiating brucella abortus into three categories: field strains, vaccine strain 19 (s19), and vaccine strain rb51/parent strain 2308 (s2308). two hundred thirty-one isolates were identified and tested by the conventional biochemical tests and brucella amos pcr. this included 120 isolates ... | 2000 | 10921983 |
| sensitivity and specificity of an elisa as a screening test for the diagnosis of brucella ovis in sheep. | a hot saline extract of brucella ovis strain reo 198 at a concentration of 5 micrograms/ml in phosphate buffer ph 7.2 was used to adsorb onto maxisorb plates and incubated at 37 degrees c during 12 h; unadsorbed excess antigen was washed off thrice with phosphate buffer containing 0.5% tween 20. as blocking agent 1% skim milk was used. the conjugate used was protein g bound to peroxidase diluted 1:100. thirty three sheep sera from bacteriologically confirmed infected animals and 39 sheep sera fr ... | 1997 | 10932721 |
| evaluation of tests employed in serological diagnosis of brucellosis caused by brucella ovis. | a survey was carried out to verify the sensitivity and specificity of various tests (complement fixation test--cf; agar gel immunodiffusion--agid; indirect enzyme linked immunosorbent assay--elisa; immunoblotting--ib) employed in the serological diagnosis of brucellosis caused by brucella ovis. the tests were executed on 44 blood serum samples of rams coming from b. ovis-free flocks, 75 of b. ovis experimentally infected rams and 1139 from rams living in flocks where b. ovis had been previously ... | 2000 | 10939043 |
| a homologue of an operon required for dna transfer in agrobacterium is required in brucella abortus for virulence and intracellular multiplication. | as part of a brucella abortus 2308 genome project carried out in our laboratory, we identified, cloned, and sequenced a genomic dna fragment containing a locus (virb) highly homologous to bacterial type iv secretion systems. the b. abortus virb locus is a collinear arrangement of 13 open reading frames (orfs). between virb1 and virb2 and downstream of orf12, two degenerated, palindromic repeat sequences characteristic of brucella intergenic regions were found. gene reporter studies demonstrated ... | 2000 | 10940027 |
| an immunoblotting technique for the serodiagnosis of brucellosis by brucella ovis. | an immunoblotting (ib) technique was developed for the serodiagnosis of brucellosis caused by brucella ovis. immunoblotting was performed, using a b. ovis hs (hot saline extract) antigen, on 44 blood serum samples which came from rams belonging to known brucella-free flocks, 114 samples originating from ten experimentally b. ovis infected rams and 100 from rams of naturally b. ovis infected flocks. no bands were noted on any of the 44 serum samples which originated from known negative flocks. se ... | 2000 | 10946406 |
| an is711 element downstream of the bp26 gene is a specific marker of brucella spp. isolated from marine mammals. | dna polymorphism of the bp26 gene, coding for a diagnostic protein antigen for brucellosis, was assessed by pcr and restriction fragment length polymorphism analysis using primers to amplify the bp26 gene with its flanking regions. surprisingly, whereas pcr performed on dna of the reference strains of the six recognized brucella species produced a product of the expected size (1,029 bp), pcr performed on dna of three representative strains from marine mammals (from a seal, a dolphin, and a porpo ... | 2000 | 10973465 |
| identification and characterization of the brucella abortus phosphoglucomutase gene: role of lipopolysaccharide in virulence and intracellular multiplication. | smooth lipopolysaccharide (lps) of brucella abortus has been reported to be an important virulence factor, although its precise role in pathogenesis is not yet clear. while the protective properties of lps against complement are well accepted, there is still some controversy about the capacity of rough mutants to replicate intracellularly. the b. abortus phosphoglucomutase gene (pgm) was cloned, sequenced, and disrupted. the gene has a high index of identity to agrobacterium tumefaciens pgm but ... | 2000 | 10992476 |
| limited genetic diversity of brucella spp. | multilocus enzyme electrophoresis (mlee) of 99 brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. in mlee-derived dendrograms, brucella abortus and a marine brucella sp. grouped into a single electrophoretic type related to brucella neotomae and brucella ovis. brucella suis and brucella canis formed another cluster linked to brucella melitensis and related to rhizobium tropici. th ... | 2001 | 11136777 |
| brucella abortus rb51 and hot saline extract from brucella ovis as antigens in a complement fixation test used to detect sheep vaccinated with brucella abortus rb51. | the efficacy of brucella abortus rb51 and hot saline extract (hse) from brucella ovis as antigens in complement fixation (cf) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with b. abortus strain rb51. for this study, four 5-month-old sheep were vaccinated subcutaneously with 5 x 10(9) cfu of rb51, and two sheep received saline. serum samples collected at different times after vaccination were tested for the presence of antibodies to rb51 by a cf test with rb ... | 2001 | 11139204 |
| deletion of wboa enhances activation of the lectin pathway of complement in brucella abortus and brucella melitensis. | brucella spp. are gram-negative intracellular pathogens that survive and multiply within phagocytic cells of their hosts. smooth organisms present o polysaccharides (ops) on their surface. these ops help the bacteria avoid the bactericidal action of serum. the wboa gene, coding for the enzyme glycosyltransferase, is essential for the synthesis of o chain in brucella. in this study, the sensitivity to serum of smooth, virulent brucella melitensis 16m and b. abortus 2308, rough wboa mutants vtrm1, ... | 2001 | 11401980 |
| brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication in hela cells. | null cyclic beta-1,2-glucan synthetase mutants (cgs mutants) were obtained from brucella abortus virulent strain 2308 and from b. abortus attenuated vaccinal strain s19. both mutants show greater sensitivity to surfactants like deoxycholic acid, sodium dodecyl sulfate, and zwittergent than the parental strains, suggesting cell surface alterations. although not to the same extent, both mutants display reduced virulence in mice and defective intracellular multiplication in hela cells. the b. abort ... | 2001 | 11401996 |
| major outer membrane protein omp25 of brucella suis is involved in inhibition of tumor necrosis factor alpha production during infection of human macrophages. | brucella spp. can establish themselves and cause disease in humans and animals. the mechanisms by which brucella spp. evade the antibacterial defenses of their host, however, remain largely unknown. we have previously reported that live brucellae failed to induce tumor necrosis factor alpha (tnf-alpha) production upon human macrophage infection. this inhibition is associated with a nonidentified protein that is released into culture medium. outer membrane proteins (omps) of gram-negative bacteri ... | 2001 | 11447156 |
| a brucella ovis antigenic complex bearing poly-epsilon-caprolactone microparticles confer protection against experimental brucellosis in mice. | a hot saline antigenic extract (hs) from brucella ovis was encapsulated in poly-epsilon-caprolactone microparticles (pec), and tested as a vaccine against b. ovis and b. abortus infections in mice. subcutaneous but not oral administration in balb/c mice of the hs-pec induced high amounts of ifn-gamma and il-2 but low quantities of il-4 suggesting a combined th1/th2 cellular immune response. the vaccine administered either subcutaneously or orally protected mice against b. ovis infection. such pr ... | 2001 | 11457533 |
| induction of specific cytotoxic lymphocytes in mice vaccinated with brucella abortus rb51. | a safe, more sensitive, nonradioactive, neutral red uptake assay was adopted to replace the traditional 51cr release assay for detection of brucella-specific cytotoxic t lymphocyte (ctl) activity. our studies indicated that brucella abortus strain rb51 vaccination of mice induced specific ctls against both strain rb51- and strain 2308-infected j774.a1 macrophages but not against listeria monocytogenes-infected j774.a1 cells. the antigen-specific cytotoxic activity was exerted by t lymphocytes bu ... | 2001 | 11500423 |
| cloning, nucleotide sequence, and expression of the brucella melitensis sucb gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein. | the brucella melitensis sucb gene encoding the dihydrolipoamide succinyltransferase (e2o) enzyme (previously identified as an immunogenic protein in infected sheep) was cloned and sequenced. the amino acid sequence predicted from the cloned gene revealed 88.8 and 51.2% identity to the dihydrolipoamide succinyltransferase sucb protein from brucella abortus and escherichia coli, respectively. sera from naturally infected sheep showed antibody reactivity against the recombinant sucb protein. | 2001 | 11553602 |
| mouse cytokine profiles associated with brucella abortus rb51 vaccination or b. abortus 2308 infection. | this study indicated that mice immunized with brucella abortus rb51 bacteria and subsequently challenged with b. abortus 2308 were protected from reinfection. after vaccination, both th1 and th2 cytokine patterns were observed. of those, the early production of gamma interferon seems to have the prominent role in inducing an immunologically based protection. | 2001 | 11553603 |
| characterization of a brucella species 25-kilobase dna fragment deleted from brucella abortus reveals a large gene cluster related to the synthesis of a polysaccharide. | in the present study we completed the nucleotide sequence of a brucella melitensis 16m dna fragment deleted from b. abortus that accounts for 25,064 bp and show that the other brucella spp. contain the entire 25-kb dna fragment. two short direct repeats of four nucleotides, detected in the b. melitensis 16m dna flanking both sides of the fragment deleted from b. abortus, might have been involved in the deletion formation by a strand slippage mechanism during replication. in addition to omp31, co ... | 2001 | 11598046 |
| minor nucleotide substitutions in the omp31 gene of brucella ovis result in antigenic differences in the major outer membrane protein that it encodes compared to those of the other brucella species. | the gene coding for the major outer membrane protein omp31 was sequenced in five brucella species and their biovars. although the omp31 genes appeared to be highly conserved in the genus brucella, nine nucleotide substitutions were detected in the gene of brucella ovis compared to that of brucella melitensis. as shown by differential binding properties of monoclonal antibodies (mabs) to the two brucella species, these nucleotide substitutions result in different antigenic properties of omp31. th ... | 2001 | 11598077 |
| congeners of smap29 kill ovine pathogens and induce ultrastructural damage in bacterial cells. | smap29, an ovine cathelicidin, was systematically altered to create a family of 23 related peptides for mic and minimum bactericidal concentration determinations. smap28, smap29, and a derivative of smap29 called ovispirin were all antimicrobial. however, many congeners of smap29 and ovispirin were not as active as the parent molecules. with immunoelectron microscopy, smap29 was seen on membranes and within the cytoplasm of pseudomonas aeruginosa pao1. | 2001 | 11600395 |
| the genome sequence of the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and malta fever in humans. the genome of b. melitensis strain 16m was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 orfs. by using the bioinformatics suite ergo, 2,487 (78%) orfs were assigned functions. the origins of replication of the two chromosomes are similar to those of other alpha-proteobacter ... | 2002 | 11756688 |
| the genome sequence of the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and malta fever in humans. the genome of b. melitensis strain 16m was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 orfs. by using the bioinformatics suite ergo, 2,487 (78%) orfs were assigned functions. the origins of replication of the two chromosomes are similar to those of other alpha-proteobacter ... | 2002 | 11756688 |
| diagnostic usefulness of antibodies against ribosome recycling factor from brucella melitensis in human or canine brucellosis. | the diagnostic usefulness of an enzyme-linked immunosorbent assay (elisa) using a purified recombinant ribosome recycling factor from brucella melitensis (cp24 antigen) was tested in human and canine infections caused by smooth and rough brucella species, respectively. anti-cp24 antibodies were detected in 9 (43%) of 21 consecutive cases of canine brucellosis and in 8 (53%) of 15 dogs followed for 60 days after the diagnosis of acute brucellosis. among eight patients with acute brucellosis, anti ... | 2002 | 11874879 |
| experimental brucella ovis infection in mouflon (ovis musimon). | brucella ovis was isolated for the first time in italy in 1994 from the genital organs of two domestic rams. in subsequent years bacteriologic and serologic investigations demonstrated an increasing distribution of this disease in domestic sheep. mouflon (ovis musimon) occur in several hilly and mountainous areas of italy where they can potentially contact domestic sheep. to determine if this species may have a role in the epidemiology of b. ovis, four male and four female mouflon, serologically ... | 2002 | 12038127 |
| single-step purification and evaluation of recombinant bp26 protein for serological diagnosis of brucella ovis infection in rams. | to investigate the value of the bp26 protein in the serological diagnosis of ovine brucellosis caused by brucella ovis, recombinant bp26 protein was produced in echerichia coli and purified for use in an indirect enzyme-linked immunosorbent assay (i-elisa). the majority of the recombinant protein was recovered from the supernatant of sonicated recombinant e. coli cells in a soluble form. this facilitated the purification of the recombinant bp26 protein which was achieved by using ion-exchange ch ... | 2002 | 12052332 |
| polyester microparticles as a vaccine delivery system for brucellosis: influence of the polymer on release, phagocytosis and toxicity. | microparticles, containing an antigenic complex from brucella ovis (hs), were evaluated for vaccine purposes against brucellosis. they were prepared by the double emulsion solvent evaporation method using two different polyesters, poly-lactide-co-glycolide acid (75:25; rg 756) and poly-epsilon-caprolactone. the encapsulation efficiency and release of hs from the microparticles, their capacity to be phagocytosed and also their toxicity on murine monocytes j774.2 were investigated. both polymers l ... | 2002 | 12075822 |
| brucella species lacking the major outer membrane protein omp25 are attenuated in mice and protect against brucella melitensis and brucella ovis. | to aid in the development of novel efficacious vaccines against brucellosis, omp25 was examined as a potential candidate. to determine the role of omp25 in virulence, mutants were created with brucella abortus (ba25), brucella melitensis (bm25), and brucella ovis (bo25) which contain disruptions in the omp25 gene (deltaomp25 mutants). western immunoblot analysis and pcr verified that the omp25 protein was not expressed and that the omp25 gene was disrupted in each strain. balb/c mice infected wi ... | 2002 | 12151196 |
| development of microparticles prepared by spray-drying as a vaccine delivery system against brucellosis. | the antigenic extract hot saline from brucella ovis was microencapsulated by the spray-drying technique with different polyesters (poly-lactide-co-glycolide rg502h [plga], and blends with poly- epsilon -caprolactone [pec]) in order to obtain microparticles smaller than 5 microm. microparticles were tested for encapsulation efficiency, release studies, acidification of the in vitro release medium, and in vitro j744-macrophage experiments (phagocytosis and toxicity of the preparations) to determin ... | 2002 | 12176275 |
| effect of omp10 or omp19 deletion on brucella abortus outer membrane properties and virulence in mice. | the distinctive properties of brucella outer membrane have been considered to be critical for brucella sp. virulence. among the outer membrane molecules possibly related to these properties, omp10 and omp19 are immunoreactive outer membrane lipoproteins. moreover, these proteins of brucella could constitute a new family of outer membrane proteins specifically encountered in the family rhizobiaceae: we evaluated the impact of omp10 or omp19 deletion on brucella abortus outer membrane properties a ... | 2002 | 12228280 |
| an overview of the eradication of brucella melitensis from kwazulu-natal. | brucella melitensis is a gram-negative bacterium whose primary hosts are goats and sheep. like the other brucelia spp., with the exception of brucella ovis, it is not particularly host specific as it is pathogenic for a variety of other mammal species including humans. in humans the disease caused by it is rated as one of the most important zoonoses. three outbreaks have been recorded in goats and sheep in south africa; the first outbreak occurred in sheep in 1965 in the mpumalanga and northern ... | 2002 | 12233997 |
| comparison of serological tests based on outer membrane or internal antigens for detecting antibodies to brucella ovis in infected flocks. | the aim of this work was to compare the performance of 6 serological tests using outer or internal antigens from brucella for the diagnosis of brucella ovis infection in sheep in an endemic area. outer membrane antigens included a hot saline extract (hs) and the rough lipopolysaccharide (r-lps) from b. ovis. internal antigens were lps-free total cytosolic proteins (cp) and an 18-kda cytosolic protein (p18) from brucella spp. sera from 200 sheep from naturally infected flocks were assayed by agar ... | 2002 | 12296393 |
| combined s99/rb51 antigen for complement fixation test for serological diagnosis of brucellosis in cattle and sheep. | to assess the efficiency of a single antigen for the complement fixation (cf) test, prepared by combining brucella abortus smooth strain 99 (s99) with brucella abortus rough strain rb51(rb51), in detecting cattle and sheep infected or vaccinated with brucella spp. | 2002 | 12392535 |
| the analysis of the intramacrophagic virulome of brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. | the pathogen brucella suis resides and multiplies within a phagocytic vacuole of its host cell, the macrophage. the resulting complex relationship has been investigated by the analysis of the set of genes required for virulence, which we call intramacrophagic virulome. ten thousand two hundred and seventy-two minitn5 mutants of b. suis constitutively expressing gfp were screened by fluorescence microscopy for lack of intracellular multiplication in human macrophages. one hundred thirty-one such ... | 2002 | 12438693 |
| modulation of the cellular immune response after oral or subcutaneous immunization with microparticles containing brucella ovis antigens. | an antigenic extract (hs) from brucella ovis was encapsulated in either poly-epsilon-caprolactone (pec) or poly-lactic-co-glycolic acid 75:25 (plga) microparticles containing beta-cyclodextrin and pluronic f-68 as stabilising agents. the resulting microparticles displayed sub-5 microm sizes. antigen loading was 5.2 and 3.8 microg/mg for hs-pec and hs-plga microparticles, respectively. specific hs cytokine profiles were determined after subcutaneous and oral immunisation of balb/c mice. gut distr ... | 2002 | 12480328 |
| evaluation of a pcr test for the diagnosis of brucella ovis infection in semen samples from rams. | the sensitivity and specificity of a pcr assay with primers derived from the insertion sequence is6501 was compared with that of bacteriological culture and serological tests for the diagnosis of brucella ovis infection in rams. no amplifications were detected with dnas from the strains phylogenetically related to brucella and from the seven bacterial species considered as the main etiologic agents of epididymitis in rams. in addition, the specificity of the pcr was 100% when testing semen sampl ... | 2003 | 12488071 |
| serological diagnosis of brucellosis caused by brucella canis. | blood serum samples from 2,328 dogs were tested to detect antibodies against brucella canis with the agar gel immunodiffusion (agid) and 2-mercaptoethanol slide agglutination test (me-sat) using brucella ovis as the antigen. all blood serum samples were also evaluated for antibodies against brucella abortus and brucella melitensis using the rose bengal test. twentyfive (1.07%) of the sera evaluated were considered positive with agid test. only 4 (16%) of these blood serum samples were positive w ... | 2003 | 12578313 |
| production of the type iv secretion system differs among brucella species as revealed with virb5- and virb8-specific antisera. | expression of the virb operon, encoding the type iv secretion system required for brucella suis virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic ph values. to analyze the production of virb proteins, polyclonal antisera against b. suis virb5 and virb8 were generated. western blot analysis revealed that virb5 and virb8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation. unl ... | 2003 | 12595417 |
| role of the brucella suis lipopolysaccharide o antigen in phagosomal genesis and in inhibition of phagosome-lysosome fusion in murine macrophages. | brucella species are gram-negative, facultative intracellular bacteria that infect humans and animals. these organisms can survive and replicate within a membrane-bound compartment inside professional and nonprofessional phagocytic cells. inhibition of phagosome-lysosome fusion has been proposed as a mechanism for intracellular survival in both cell types. however, the molecular mechanisms and the microbial factors involved are poorly understood. smooth lipopolysaccharide (lps) of brucella has b ... | 2003 | 12595466 |
| the recombinant omp31 from brucella melitensis alone or associated with rough lipopolysaccharide induces protection against brucella ovis infection in balb/c mice. | immunogenicity and protective activity against brucella ovis of detergent-extracted recombinant omp31 (romp31 extract) from brucella melitensis produced in escherichia coli, purified rough lipopolysaccharide from b. ovis (r-lps) and a mixture of romp31 extract and r-lps (romp31 extract + r-lps) were assessed in balb/c mice. the experimental vaccines were compared with a hot saline extract (hs extract) from b. ovis mainly composed of outer membrane proteins (omps) and r-lps, and known to be prote ... | 2003 | 12650766 |
| type iii secretion homologs are present in brucella melitensis, b. ovis, and b. suis biovars 1, 2, and 3. | protein sequences from characterized type iii secretion (tts) systems were used as probes in silico to identify several tts gene homologs in the genome sequence of brucella suis biovar 1 strain 1330. four of the genes, named flhb, flip, flir, and flif on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in pcr and hybridization assays to determine their distribution among other brucella nomen species and biovars. the results indicated that flhb, flip, flir and ... | 2003 | 12732970 |
| characterization of brucella abortus o-polysaccharide and core lipopolysaccharide mutants and demonstration that a complete core is required for rough vaccines to be efficient against brucella abortus and brucella ovis in the mouse model. | brucella abortus rough lipopolysaccharide (lps) mutants were obtained by transposon insertion into two wbk genes (wbka [putative glycosyltransferase; formerly rfbu] and per [perosamine synthetase]), into manb (pmm [phosphomannomutase; formerly rfbk]), and into an unassigned gene. consistent with gene-predicted roles, electrophoretic analysis, 2-keto-3-manno-d-octulosonate measurements, and immunoblots with monoclonal antibodies to o-polysaccharide, outer and inner core epitopes showed no o-polys ... | 2003 | 12761107 |
| association of maedi visna virus with brucella ovis infection in rams. | maedi visna virus (mvv) is the etiological agent of a systemic disease of sheep, which causes lesions in lungs, the central nervous system, joints, and mammary glands. it has been speculated that the association with brucella ovis may lead to the venereal shedding of the virus. in this work, samples of epididymis from ten rams positive for mvv and infected experimentally with brucella ovis, were subjected to liquid-phase pcr, immunohistochemistry (ihc) and in situ pcr tests, aimed at identifying ... | 2003 | 12777212 |
| identification and characterization of transposable elements of paracoccus pantotrophus. | we studied diversity and distribution of transposable elements residing in different strains (dsm 11072, dsm 11073, dsm 65, and lmd 82.5) of a soil bacterium paracoccus pantotrophus (alpha-proteobacteria). with application of a shuttle entrapment vector pmec1, several novel insertion sequences (iss) and transposons (tns) have been identified. they were sequenced and subjected to detailed comparative analysis, which allowed their characterization (i.e., identification of transposase genes, termin ... | 2003 | 12813068 |
| epitope mapping of the brucella melitensis bp26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. | sequencing of bp26, the gene encoding the brucella sp. immunogenic bp26 periplasmic protein, was performed in the reference strains of brucella abortus, b. suis, and b. ovis. the three bp26 sequences were almost identical to that published for b. melitensis 16m bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. the bp26 genes of the seven b. abortus biovar reference strains and b. abortus s19 and rb51 vaccine strains were also ... | 2003 | 12853399 |
| brucella 'hoof-prints': strain typing by multi-locus analysis of variable number tandem repeats (vntrs). | currently, there are very few tools available for subtyping brucella isolates for epidemiological trace-back. subtyping is difficult because of the genetic homogeneity within the genus. sequencing of the genomes from three brucella species has facilitated the search for dna sequence variability. recently, hypervariability among short tandem repeat sequences has been exploited for strain-typing of several bacterial pathogens. | 2003 | 12857351 |
| studies on brucella ovis (n.sp.), a cause of genital disease of sheep in new zealand and australia. | | 1956 | 13367402 |
| placental pathology. i. placental lesions of sheep experimentally infected with brucella ovis. | | 1963 | 14080519 |
| comparative distribution of brucella abortus, brucella melitensis, and brucella ovis in experimentally infected pregnant sheep. | | 1964 | 14266901 |
| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
| molecular characterization of brucella abortus chromosome ii recombination. | large-scale genomic rearrangements including inversions, deletions, and duplications are significant in bacterial evolution. the recently completed brucella melitensis 16m and brucella suis 1330 genomes have facilitated the investigation of such events in the brucella spp. suppressive subtractive hybridization (ssh) was employed in identifying genomic differences between b. melitensis 16m and brucella abortus 2308. analysis of 45 ssh clones revealed several deletions on chromosomes of b. abortus ... | 2003 | 14526025 |
| [evaluation of 2 hemoculture media for the isolation of brucella spp.]]. | the diagnostic efficiency of two hemoculture media for the detection of different species of brucella strains was evaluated. strains of brucella melitensis, brucella suis, brucella abortus, brucella ovis, and brucella abortus s19 were used. each strain was diluted in phosphate buffer saline (pbs) to obtain a concentration of 10(5) colony forming units/ml (cfu/ml). blood from goats, pigs, cattle, and sheep was mixed with the bacterial suspension to obtain a final concentration minor or equal to 1 ... | 2003 | 14587372 |
| identification and distribution of insertion sequences of paracoccus solventivorans. | three novel insertion sequences (iss) (ispso1, ispso2, and ispso3) of the soil bacterium paracoccus solventivorans dsm 11592 were identified by transposition into entrapment vector pmec1. ispso1 (1,400 bp) carries one large open reading frame (orf) encoding a putative basic protein (with a dde motif conserved among transposases [tnps] of elements belonging to the is256 family) with the highest levels of similarity with the hypothetical tnps of rhodospirillum rubrum and sphingopyxis macrogoltabid ... | 2003 | 14660342 |
| brucella abortus rough mutants are cytopathic for macrophages in culture. | rough mutants of brucella spp. are attenuated for survival in animal models. however, conflicting in vitro evidence has been obtained concerning the intracellular survival of rough mutants. transposon-derived rough mutants isolated in our laboratory were previously shown to exhibit small but significant reductions in intracellular survival in a 12-h in vitro assay. several recent publications report that rough mutants exhibited increased macrophage uptake relative to their smooth parental strain ... | 2004 | 14688125 |
| antibody reactivity to omp31 from brucella melitensis in human and animal infections by smooth and rough brucellae. | group 3 of outer membrane proteins (omps) of brucella includes omp25 and omp31, which share 34% identity. omp25 is highly conserved in brucella species, and omp31 is present in all brucella species, except brucella abortus. antibodies to brucella melitensis omp31 have been sought only in infected sheep, and western blotting of sera from infected sheep did not reveal anti-omp31 reactivity. we obtained recombinant purified omp31 (b. melitensis) and tested its recognition by sera from humans and an ... | 2004 | 14715555 |
| bartholin's gland abscess caused by brucella melitensis. | we report herein a case of bartholin's gland abscess caused by brucella melitensis. clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a brucellar etiology in a broad range of clinical settings. | 2004 | 14766890 |
| influence of the co-encapsulation of different excipients on the properties of polyester microparticle-based vaccine against brucellosis. | this work evaluates the influence of different pharmaceutical auxiliaries (pluronic f68, polyvinylpyrrolidone [pvp] or tween 20), when mixed with an antigenic extract from brucella ovis (hot saline; hs), on the characteristics of the resulting poly(epsilon-caprolactone) (pec) and poly(lactide-co-glycolide) (plga) microparticles. in all cases, pec microparticles were smaller than plga ones. concerning the hs loading, plga microparticles were highly dependent on the type of the excipient used, whe ... | 2004 | 15129979 |
| lack of evidence of brucella ovis infection in rams in quebec. | a study was conducted to estimate the seroprevalence of brucella ovis infection in rams in the estrie and bas-saint-laurent regions (quebec). rams sera (n = 258) were serologically evaluated from 224 rams in 30 commercial flocks and from 34 rams at 2 slaughterhouses by using an enzyme linked immunosorbent assay. epididymides and testes were examined by palpation on farms and microscopically for culled rams. no ram was seropositive to brucella ovis or had lesions suggestive of brucellosis from th ... | 2004 | 15144103 |
| development and evaluation as vaccines in mice of brucella melitensis rev.1 single and double deletion mutants of the bp26 and omp31 genes coding for antigens of diagnostic significance in ovine brucellosis. | the live attenuated brucella melitensis rev.1 strain is considered the best vaccine available for the prophylaxis of brucellosis in sheep caused by either b. melitensis or brucella ovis. however, its application stimulates antibody responses in vaccinated animals indistinguishable by the current conventional serological tests from those observed in infected animals. the periplasmic protein bp26 and the outer membrane protein (omp) omp31 are immunodominant antigens in the serological responses of ... | 2004 | 15246618 |
| occurrence and potential diagnostic applications of serological cross-reactivities between brucella and other alpha-proteobacteria. | agrobacterium, sinorhizobium, and ochrobactrum are genera closely related to brucella but, in contrast to the latter, are not pathogenic for humans and animals. we studied by an indirect enzyme-linked immunosorbent assay (elisa) the reactivities of brucellosis sera against cytosolic (cyt) and membrane (ma) antigens from these nonpathogenic bacteria, and we evaluated the potential usefulness of these cross-reactions for the diagnosis of brucellosis in humans, sheep, cows, and dogs. canine infecti ... | 2004 | 15358645 |
| dna polymorphism in the omp25/omp31 family of brucella spp.: identification of a 1.7-kb inversion in brucella cetaceae and of a 15.1-kb genomic island, absent from brucella ovis, related to the synthesis of smooth lipopolysaccharide. | five genes homologous to the well-known omp25 and omp31 genes, that code for two major brucella spp. outer membrane proteins (omps), have been detected in the genome of brucella melitensis 16m and brucella suis 1330. in this work we have determined the nucleotide sequence of these five genes, named omp31b, omp25b, omp25c, omp25d and omp22, in the six classical brucella species reference strains and in representative strains of the recently proposed species brucella cetaceae and brucella pinniped ... | 2004 | 15374004 |
| genetic bases of the rifampin resistance phenotype in brucella spp. | rifampin is one of the most potent and broad-spectrum antibiotics against bacterial pathogens. its bactericidal activity is due to its ability to bind to the beta subunit of the dna-dependent rna polymerase encoded by the rpob gene. mutations of the rpob gene have been characterized in rifampin-resistant (rif(r)) strains of escherichia coli and mycobacterium tuberculosis. the genetic bases of rif(r) in brucella spp. are still unknown. in the present study, the nucleotide sequences of the rpob ge ... | 2004 | 15583262 |
| efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to yersinia enterocolitica o:9. | yersinia enterocolitica o:9 bears a smooth lipopolysaccharide (s-lps) of brucella sp. o-chain a+c/y epitopic structure and is a cause of false-positive serological reactions (fpsr) in standard tests for cattle brucellosis. brucella s-lps, cross-reacting s-lpss representing several o-chain epitope combinations, brucella core lipid a epitopes (rough lps), brucella abortus s-lps-derived polysaccharide, native hapten polysaccharide, rough lps group 3 outer membrane protein complexes, recombinant bp2 ... | 2005 | 15642999 |
| use of brucella canis antigen for detection of ovine serum antibodies against brucella ovis. | brucella ovis causes a genital disease of sheep manifested by epididymitis in rams and placentitis in ewes producing reduced fertility in the flock. clinical diagnosis is not sensitive enough and bacteriological testing is not feasible for detection of the disease in large numbers of animals. indirect methods of serological testing are preferred for routine diagnosis, of which agar gel immunodiffusion (agid), complement fixation (cf) and elisa tests are recommended as the most efficient. since b ... | 2004 | 15708814 |
| subcellular fractions of brucella ovis distinctively induce the production of interleukin-2, interleukin-4, and interferon-gamma in mice. | the aim of this study was to evaluate the effect of 3 brucella ovis subcellular protein fractions: outer membrane (omp), inner membrane (imp), and cytoplasm (cp), on cellular immune response by in vitro production of interleukin (il)-2, il-4, and interferon (ifn)-gamma. each fraction was inoculated 3 times into balb/c mice, primary cultures of mice spleen cells were done, and these were then stimulated with the fractions. culture supernatants were collected at 24, 48, 72, 96, and 120 h postinocu ... | 2005 | 15745223 |
| rhizobial 16s rrna and dnak genes: mosaicism and the uncertain phylogenetic placement of rhizobium galegae. | the phylogenetic relatedness among 12 agriculturally important species in the order rhizobiales was estimated by comparative 16s rrna and dnak sequence analyses. two groups of related species were identified by neighbor-joining and maximum-parsimony analysis. one group consisted of mesorhizobium loti and mesorhizobium ciceri, and the other group consisted of agrobacterium rhizogenes, rhizobium tropici, rhizobium etli, and rhizobium leguminosarum. although bootstrap support for the placement of t ... | 2005 | 15746335 |
| restriction site polymorphisms in the genes encoding new members of group 3 outer membrane protein family of brucella spp. | thirty-seven brucella reference and field strains representing all the species and their biovars were analysed by pcr-rflp to determine the degree of variation in the genes encoding the new members of group 3 outer membrane protein (omp) family. analysis of the omp22 and omp25c/omp25d genes indicated that the restriction patterns were identical for all species and biovars with all restriction enzymes tested, except for brucella ovis that showed a short 30 bp deletion close to omp22 gene, and for ... | 2005 | 15796983 |
| demonstration of polymorphism among brucella ovis field isolates by pulsed-field gel electrophoresis. | brucella ovis is recognized worldwide as an important pathogen of sheep, and has also been identified in farmed deer in new zealand. previously, only one strain type of b. ovis has been identified. the objective of this paper was to perform pulsed-field gel electrophoresis (pfge) on field isolates of b. ovis to determine whether strain variations exist, whether sheep and deer are affected by the same strains, and to compare the performance of the rare-cutting restriction enzymes xbai and swai. t ... | 2005 | 15871911 |
| the persistence of antibodies against brucella ovis and brucella abortus in rams following vaccination: a field study. | | 1967 | 16030713 |
| the complement fixation test for brucella ovis. | | 1982 | 16030829 |
| further practitioner comment on brucella ovis. | | 1982 | 16030847 |
| brucella ovis eradication. | | 1982 | 16030863 |
| serology as an aid to diagnosis: uses and abuses. | the importance of correct interpretation of serological test results, common sources of error and problems associated with tests are discussed. in bovine brucellosis, a disease which is ideally suited to serological diagnosis, foetal contact with infection may cause the calf to be a serologically negative carrier. the immune tolerant animal resulting from foetal contact with virus is a major problem in the serological detection of border disease. in johnes' disease and to a lesser extent in bruc ... | 1982 | 16030880 |
| the brucella ovis complement fixation test. | | 1983 | 16030984 |
| the complement fixation test for the diagnosis of brucella ovis infection in rams--an animal health division report. | complement fixation tests using three b. ovis antigen preparations in warm fixation tests (wcft) and cold fixation (ccft) tests were done on 541 ram sera. semen samples from the same rams were examined culturally to identify b. ovis excretors. the ccft, using an antigen prepared by heat extraction of b. ovis cells, had a sensitivity of 97% in 124 rams which were shedding b.ovis. the specificity was 99% in 144 rams from non-infected flocks. seventy-seven per cent of 156 rams which reacted to this ... | 1983 | 16030997 |