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an abattoir survey of bull genitalia.this paper describes a survey of 504 bull genitalia collected after slaughter at 3 western australian abattoirs over 18 months. details of the gross pathology, histopathology, and microbiology of the genitalia, and physical dimensions of the testicles are presented. the major breeds represented were hereford (30%), shorthorn (16%), friesian (14.3%), and aberdeen angus (13.9%). the mean age of 263 bulls (52.6%) was 4.4 years. the mean length and breadth of 469 paired testicles was 10.8 cm and 6.9 ...1977901333
campylobacter-associated diarrhoea in southampton. 1977912384
campylobacter-associated diarrhoea in edinburgh. 1977912385
porcine intestinal adenomatosis associated with serologically distinct campylobacter sputorum subspecies mucosalis.a variant of campylobacter sputorum subspecies mucosalis which is serologically distinct from all strains previously examined is described. it has been isolated from cases of porcine intestinal adenomatosis and necrotic enteritis and in all other characteristics conforms to the description of mucosalis.1977928990
studies on campylobacter sputorum subspecies mucosalis.catalase-negative vibrios can be isolated in large numbers from the affected intestinal mucosa of pigs suffering from a range of porcine enteropathies in which the mucosa has an adenomatous component. these vibrios cannot be distinguished from strains of campylobacter sputorum subsp. mucosalis. the resemblance between these bacteria strengthens the case already made on morphological evidence that these enteropathies have a common origin. catalase-negative vibrios have also been isolated from the ...1976933148
[biochemical and serological investigations of flagellae of campylobacter fetus (author's transl)].flagellae of campylobacter fetus group o, types 1, 2 and 7 were prepared. first they were separated from cell bodies using an ultramix. the suspension was then centrifuged for 20 mins. at 10000 rpm and the supernatant frozen at -40 degrees c. this is a simple method for the enrichment of preparations of flagellae, as they become tangled up and accumulate in the inferior third part of the frozen liquid. the physicochemical basis of this phenomenon was discussed. after thawing and spinning for 20 ...1976936824
[epidemiologic studies on the vibrio fetus infection of cattle]. 1976938446
stimulatory effect of dihydroxyphenyl compounds on the aerotolerance of spirillum volutans and campylobacter fetus subspecies jejuni.the aerotolerance of the microaerophilic bacterium spirillum volutans was greatly stimulated in a defined medium by the presence of dihydroxyphenyl ferric iron-binding compounds such as nor-epinephrine at 10(-5) to 10(-6) m. dihydroxyphenyl compounds at 2 x 10(-4) m, or iron salts (ferrous or ferric) at high concentration, greatly increased the aerotolerance of a strain of campylobacter fetus subsp. jejuni when grown on streak plates of brucella agar. the results suggest that the microaerophilis ...1976942201
septicaemia and meningitis with campylobacter fetus subspecies intestinalis.two cases of infection with campylobacter fetus subspecies intestinalis are reported. in a 46-year-old farmer the development of septicaemic campylobacteriosis with acute gastroenterocolitis was favoured by malnutrition due to chronic alcoholism. after treatment with erythromycin the patient recovered completely. the second patient, a 40-year-old worker with meningitis, recovered after combined streptomycin-erytro-mycin therapy. in both patients antibodies against homologous and reference strain ...1976947847
campylobacterosis. 1976947862
regional ileitis in pigs isolation of campylobacter from affected ileal mucosa. 1976952257
immunisation against bovine vibriosis due to campylobacter fetus subsp. fetus biotype intermedius.a vaccine prepared from campylobacter fetus subsp. fetus biotype venerealis gave only partial protection to heifers and bulls against genital infection with c. fetus subsp. fetus biotype intermedius. heifers and bulls were fully immunised against c. fetus subsp. venerealis biotype intermedius by administration of a vaccine prepared from homologous organisms.1976985256
nature of antibodies to campylobacter fetus in preputial secretions from a vaccinated bull. 1976990892
[study of the specificity of the agglutination reaction in the diagnosis of ovine vibriosis]. 1976997251
a simple technique for mass cultivation of campylobacter fetus.studies using 86 media for maximum growth of campylobacter fetus for antigen production showed that a diphasic medium (solid base with liquid overlay) was most suitable. the solid base was double strength cystine heart agar. the liquid overlay was thioglycollate medium of brewer (135-c) without agar. this medium yielded maximum growth of c. fetus in six days with good motility, less clumping and less filament formation than all other media tried.19761000394
vibrio fetus endocarditis in a patient with systemic lupus erythematosus.vibrio fetus endocarditis occurred in a patient with systemic lupus erythematosus receiving azathoprin and prednisone. blood cultures required 14 days to become positive. the fastidious growth requirement of this organism is reviewed because lack of appreciation of these may result in failure to make the diagnosis. this is the first reported case of vibrio fetus endocarditis occurring in the setting of a connective tissue disorder and immunosuppressive therapy.19761015513
[oral vibrions method of isolation and demonstration of their relation to sherophorus fusiformis]. 19761020709
[the bacteriological diagnosis of vibrio fetus infection in man (author's transl)].a synopsis of the present classification of vibrio fetus is given (tab. 1, 2). besides the presentation of the importance of these bacteria in veterinary medicine, 97 human infections have been published up to now. the geographic, age and sex distribution of these cases and the material out of which the pathogens were isolated is presented (tab. 3, 4). the clinical picture is summarized. for the culture of vibrio fetus liver broth as described by tarozzi incubated for at least 3-4 days was suita ...19751041142
bilateral deep brachial vein thrombophlebitis due to vibrio fetus.a patient had bilateral deep brachial vein thrombophlebitis in which vibrio fetus was recovered from six blood cultures of the six drawn. fever and phlebitis continued with treatment with intravenous doses of heparin and oxacillin but rapidly improved with treatment change of oxacillin to clindamycin. in vitro antibiotic disk susceptibility testing confirmed resistance to oxacillin and susceptibility to clindamycin. vibrio fetus infection is associated with the vascular endothelium in this and p ...19751041482
bovine venereal vibriosis. ultrastructure of endometrial inflammatory lesions.the inflammatory lesions in bovine venereal vibriosis have been examined by light and transmission electron microscopy in order to provide a better understanding of host defense of the uterus against bacterial invasion. neutrophils and eosinophils were found mainly at the endometrial surface and in glandular lumina. mononuclear cells within the endometrial tissue were identified ultrastructurally. lymphocytes were most abundant, plasma cells were next in frequency, and mononuclear phagocytes wer ...19751080534
porcine intestinal adenomatosis: a possible relationship with necrotic enteritis, regional ileitis and proliferative haemorrhagic enteropathy.necrotic enteritis and regional ileitis are recognised causes of sporadic death in pigs. in both conditions, the intestinal mucosa harbours the bacterium, campylobacter sputorum subsp mucosalis, which was first associated with procine intestinal adenomatosis.19751080616
bovine veneral vibriosis: cure of genital infection in females by systemic immunization.cure of female cattle with venereal vibriosis by systemic immunization with killed campylobacter fetus cells in incomplete freund adjuvant was investigated. heifers infected in the cervicovaginal area with a cloned population of c. fetus venerealis were vaccinated subcutaneously 14 and 24 days thereafter with the infecting strain in incomplete freund adjuvant. six of eight vaccinated heifers were free of infection 25 to 48 days postinfection. one of the cured animals had an intercurrent infectio ...19751112614
vaccination against vibrio (campylobacter) fetus infection in sheep in late pregnancy.a single injection of vaccine containing disrupted formol-killed vibrio (campylobacter) fetus var intestinalis serotype v was shown to give good protection against abortion from an oral challenge of the same serotype administered simultaneously. protection was also afforded by vaccination at the time of the first abortion in sheep infected orally one month previously. these results suggest that vaccination subsequent to an abortion might be of value in preventing a proportion of further abortion ...19751119063
[outbreak of vibrio abortion in sheep in iraq]. 19751120061
the characterisation of campylobacter sputorum subspecies mucosalis isolated from pigs.a comparison is made between the recognised subspecies of campylobacter sputorum isolated from humans and cattle and the previously unrecognised catalase negative vibrios isolated from porcine intestinal adenomatosis. the characters of the porcine strains warrant their inclusion within the species campylobacter sputorum. differentiation between all three is possible in the laboratory and we propose that, in addition to the recognised subspecies, sputorum and bubulus, the pig strains be accorded ...19751129532
campylobacter fetus infection in humans. analysis of three cases. 19751133862
the possibility that campylobacter (vibrio) coli and treponema hyodysenteriae are both involved in swine dysentery. 19751139284
vibrio fetus septicemia in colorado. 19751145030
effects of mycoplasma spp, trichomonas fetus, and campylobacter fetus on ciliary activity of bovine uterine tube organ cultures.microscopic observations were made of sections of normal bovine uterine tube (oviduct) and fimbriae maintained in vitro and exposed to agents known to localize in the bovine genital tract, i.e., mycoplasma, trichomonas fetus, and campylobacter fetus (vibrio fetus). only c fetus stopped ciliary activity. scanning electron microscopy (sem) revealed loss of most cilia. sterile filtrate of c fetus culture had no effect.19751155827
perinatal lamb mortality in western australia: 5. vibrionic infection.vibrionic abortion was diagnosed in sheep on 48 occasions between 1963-65 and 3 of these were associated with other major abortion pathogens. the outbreaks were scattered throughout the agricultural area of western australia. time-of-death of 91 infected lambs was: ante-parturient death 50.5%, parturient death 13.2% and post-parturient death 36.3% (deaths occurring immediately after death 28.6%, deaths delayed after death 2.2%, and late post-parturient death 5.5%). the majority of lambs had gros ...19751164266
the influence of age on the susceptibility of bulls to campylobacter fetus subsp venerealis.observations were carried out on 22 hereford bulls to determine the age at which they bacame carriers of c. fetus. during preliminary investigations, 5 bulbs were repeatedly exposed to the organism by artificial or natural means from the age of 18 months. all animals became longterm carriers on reaching an age of between 40 and 70 months. prior to this, a temporary carrier state lasting from 4 to 29 days was produced in 4 bulls. in a subsequent experiment, 17 bulls of 3 different age groups were ...19751167135
bovine venereal vibriosis: activity of inflammatory cells in protective immunity.the capability of bovine polymorphonuclear (pmn) and mononuclear phagocytes to kill campylobacter (vibrio) fetus venerealis was tested under various conditions in order to judge their roles in protective immunity. bovine pmn killed c fetus in the presence of immunoglobulin g (igg) but not immunoglobulin a (iga) antibodies. some phagocytosis occurred in the absence of antibody in glass-adherent cultures, but not in suspension cultures--indicating that surface phagocytosis by the neutrophils norma ...19751173209
immunisation of cattle against vibriosis with vaccines prepared from campylobacter fetus subsp fetus.subcutaneous administration of vaccines prepared from cells of campylobacter fetus subsp fetus strain a28 to heifers gave substantial protection against infertility due to c. fetus subsp venerealis strain b6. strains a28 and b6 had different heat-stable antigens and conformed respectively to serotype b and serotype a of berg et al (1971). the results suggested that the protective antigens were heat-labile antigens common to both strains. although vaccines prepared from serotype b strains of c. f ...19751180771
isolation of campylobacter sputorum subsp mucosalis from oral cavity of pigs. 19751189242
immunosuppression in ovine trypanosomiasis. 19751216438
vibrio fetus infection. 19751235756
[classification of genus campylobacter]. 19751240297
studies on veneral transmission of campylobacter fetus by immunised bulls.temporary contamination of the penis and prepuce with campylobacter fetus subsp. venerealis and campylobacter fetus subsp. venerealis biotype intermedius may occur when immunised bulls mate with infected heifers. however, 2 experiments showed that this contamination was not a significant cause of genital infection in susceptible heifers that subsequently mated with bulls.19751243730
cystine aminopeptidase activity (oxytocinase) in pregnant guinea pigs: normal and infected with campylobacter fetus.the cystine aminopeptidase (cap) activity was determined in plasma from guinea pigs at various stages of gestation and in pregnant guinea pigs infected with campylobacter fetus subspecies intestinalis. the cap activity decreased with length of gestation, which is in contrast to the changes in activity in persons. infected guinea pigs near the time of abortion can be distinguished from normal guinea pigs on the basis of plasma cap activity, if the plasma samples are taken 1 to 2 days before abort ...19761259230
ovine campylobacterosis: preliminary studies of the efficacy of the in vitro serum bactericidal test as an assay for the potency of campylobacter (vibrio) fetus subsp intestinalis bacterins.an in vitro serum bactericidal test was developed to assess the efficacy of campylobacter fetus bacterins. four experimental monovalent bacterins (either serotype c or a-2) and 2 commercial bivalent bacterins (a "suspect" and an "efficacious" bacterin) with aluminum hydroxide adjuvant were administered to sheep and rabbits from which antiserums were then prepared. the different vaccines were evaluated by comparing the in vitro bactericidal activity of the sheep and rabbit antiserums. results of ...19761267237
t cell responses to myelin proteins in guillain-barré syndrome.we have investigated the hypothesis that the pathogenesis of guillain-barré syndrome (gbs) involves an autoimmune t cell response to p0 and p2 proteins of peripheral nerve myelin. the proliferative responses of blood mononuclear cells (mnc) to myelin proteins and synthetic peptides derived from them were determined in patients with gbs and chronic idiopathic demyelinating polyradiculoneuropathy (cidp), normal controls (nc) and patients with other neuropathies (onp). twelve out of 19 gbs patients ...19921279128
use of ribosomal rna gene restriction patterns to investigate two outbreaks of campylobacter enteritis in melbourne, australia.the analysis of ribosomal rna (rrna) gene patterns (ribotyping) has been used to differentiate strains within bacterial species. we used this method to investigate two outbreaks of campylobacter enteritis that occurred recently in melbourne, australia. the first outbreak involved seven patients although isolates from only five patients were available for typing. the second outbreak consisted of three patients infected with human immunodeficiency virus (hiv) on the same ward of a hospital. analys ...19921281233
rapid and sensitive detection of campylobacter spp. in chicken products by using the polymerase chain reaction.the polymerase chain reaction (pcr) after a short enrichment culture was used to detect campylobacter spp. in chicken products. after the 16s rrna gene sequence of campylobacter jejuni was determined and compared with known sequences from other enterobacteria, a primer and probe combination was selected from the region before v3 and the variable regions v3 and v5. with this primer set and probe, 426-bp fragments from c. jejuni, campylobacter coli, and campylobacter lari could be amplified. the d ...19921282312
the surveillance and control of campylobacter infection.campylobacter jejuni and c. coli are the most commonly reported bacterial causes of enteritis in man in the united kingdom. the reported incidence of campylobacter infection varies with place and time. its epidemiology differs from that of salmonella: campylobacters do not multiply on food, secondary spread is rare, and infections peak at the end of may (salmonellas peak in july). known sources of infection include poultry, milk, water, pets and other domestic animals. campylobacters are widespr ...19921284935
campylobacter surveillance. 19921285113
clinical features of sporadic campylobacter infections in norway.to assess risk factors and clinical impact of campylobacteriosis in norway, a case-control study of sporadic cases of infection with thermotolerant campylobacter spp. was conducted. this report describes: (1) the frequency and duration of signs and symptoms, antimicrobial treatment, hospitalization, and faecal carriage among the study patients; (2) diarrhoeal illness and campylobacter carriage among their household members; and (3) antimicrobial susceptibility pattern among bacterial isolates. a ...19921287808
introduction and spread of thermophilic campylobacters in broiler flocks.campylobacteriosis is the most commonly reported infectious cause of human gastroenteritis in developed countries and broiler chickens are considered to be the major food-borne source of the infection. the control of the infection in man depends upon its control in broiler flocks but the epidemiology in poultry is poorly understood. up to 50 per cent of broiler flocks may be infected and most of the birds in an infected flock carry the organisms until slaughter. vertical transmission through the ...19921287951
gastroenteritis outbreak at an industrial camp--british columbia. 19921291017
emergence of resistance to erythromycin and fluoroquinolones in thermotolerant campylobacter strains isolated from feces 1987-1991.during the period 1987 to 1991 a retrospective study was performed to determine the resistance of thermotolerant campylobacter species isolated from feces to erythromycin and fluoroquinolones. of the 672 strains studied, 614 (91.3%) were identified as campylobacter jejuni and 58 (8.7%) as campylobacter coli. during the study period the rate of resistance of campylobacter jejuni to erythromycin remained relatively stable (0.9-3.5%), while resistance of campylobacter coli to erythromycin emerged l ...19921291314
helicobacter pylori and peptic ulcer diseases: prevalence and association with antral gastritis in 210 patients.evidence is accumulating that helicobacter pylori infection plays a major contributory role in peptic ulcer disease [duodenal ulcer (du) and gastric ulcer (gu)] and non-ulcer dyspepsia (nud). we, therefore, studied prospectively 210 consecutive patients with upper gastrointestinal symptoms (62 du, 38 gu and 110 nud) to determine the prevalence of h. pylori infection and to investigate their association with histological gastritis. using endoscopic biopsy of the gastric antrum for diagnosing h. p ...19921293255
[the diagnostic potentials of an immunological method for detecting helicobacter (campylobacter) pylori]. 19921294826
helicobacter pylori and dyspepsia in an arab population.to determine the incidence and significance of the detection of helicobacter pylori in an arab population, 116 patients with dyspepsia were studied. 89 percent of these patients had h. pylori detected by culture or/and histological definition of campylobacter-like organisms. by the modified rapid urease test (rut) 80% of the patients had h. pylori (sensitivity of 91%, specificity of 75%). irrespective of the endoscopic diagnosis, the presence of h. pylori was associated with histologic evidence ...19921295138
campylobacter jejuni in acute diarrhoea in infancy and its relation to faecal leucocytic count.a prospective study was carried out to identify the relative risk of campylobacter jejuni infection in 50 infants with acute diarrhoea, 24 infants with acute resistant diarrhoea and 25 healthy normal infants as a matching control group. faecal samples were collected from the three groups and were cultured on both selective media for campylobacter and other media for isolation of other organisms. direct stool smears, stained with methylene blue, were examined for detection of faecal leucocyte in ...19921295942
characteristics of the internalization and intracellular survival of campylobacter jejuni in human epithelial cell cultures.the characteristics associated with the internalization and intracellular behavior of campylobacter jejuni during short-term and long-term cultivation with int 407 cells were examined. the internalization of c. jejuni by int 407 cells was inhibited by cytochalasin dansylcadaverine, chemicals that disrupt microfilament formation and inhibit receptor cycling, respectively. ammonium chloride and methylamine, two chemicals that inhibit endosomal acidification, did not affect c. jejuni internalizatio ...19921297914
in vivo effect of cadmium chloride on intestinal colonization of rats by campylobacter jejuni.campylobacter jejuni (10(8) c.f.u) fed intragastrically unto 18 laboratory rats (white whiskers) resulted in colonization of the 18 rats intestines and their eventual excretion of the organism for four months post infection. when 10(8) cfu of c. jejuni was mixed with 10 mu/ml of cadmium chloride (cdcl2) and fed immediately to another set of 18 rats, colonization of rats intestines was greatly reduced and c. jejuni was recovered from two (11%) out of 18 rats and the faeces were positive for c. je ...19921298614
[studies of outer membrane protein profiles by sds-page for campylobacter jejuni in an epidemiological investigation].the characteristics of outer membrane protein profiles of 41 strains of campylobacter jejuni from various sources by sds-page was studied. seven and nine omp patterns were differentiated respectively by the presence or absence of six outer membrane protein bands and by the number and size of the molecular weight of the major protein bands. comparing the omp patterns of the strains from human with those from animals, the authors inferred that chickens and other animals might be one of the sources ...19921298718
[the leading routes and factors in the transmission of the causative agent of campylobacteriosis under current conditions].epidemiological investigations carried out at the foci of campylobacter infection in moscow and the moscow region in 1987-1990 demonstrated that campylobacter infection was recently registered as sporadic cases in a few foci. the alimentary route of the transmission of this infection was the main factor of its spread. a high role of everyday contacts in the spread of this infection was noted. the possibility for outbreaks and sporadic cases of campylobacter infection to be masked by very frequen ...19921301659
[evaluation of antimicrobial drugs and atmospheres for the isolation of campylobacter fetus subspp. from the bovine genital tract].growth of 3 reference bovine c. fetus strains in media with and without antibiotics and bacteriostats active against the most common contaminant bacteria in the bovine genital tract was evaluated. in addition, 2 regional bovine c. fetus strains and 1 reference c. sputorum biovar bubulus strain were used in some experiments. reference strain c. fetus subsp. venerealis was completely inhibited by polymyxin (> or = 0.25 iu/ml) whereas the other c. fetus strains were not inhibited. in shepler's medi ...19921302865
analysis of cell surface antigens of campylobacter rectus.campylobacter rectus is one of the predominant bacteria in the lesions of human periodontitis. the surface antigens of the bacterium contain several components which may play significant roles in colonization and pathogenesis. a high-molecular-weight protein was selectively isolated from the cell surface of c. rectus by acid extraction and purified by deae sepharose. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the extracted protein was 150 kda. the protein was ...19921303324
[advances in the research on the pathogenic role of campylobacter jejuni in guillain-barre syndrome]. 19921305004
colonization of infant mice with flagellar variants of campylobacter jejuni.the role of flagella in the colonization of the intestine by campylobacter jejuni was investigated by challenging infant mice with two flagellated strains and their nonflagellated variants. the intestinal tracts of infant mice were regularly colonized with motile strains, but not by nonmotile variants. colonization of mice with motile c. jejuni occurred with as few as 1000 bacteria per mouse.19921307436
[detection of campylobacter species by using polymerase chain reaction and nonradioactive dna probes. ii. pcr direct sequencing of the campylobacter dna].we have developed a sensitive dna hybridization assay for the detection and identification of campylobacter species which are recognized as important pathogens of acute diarrheal disease in humans. this technique utilizes dna probes complementary to nucleotide sequences present in 16s ribosomal rna (rrna) of c. jejuni, c. coli, c. laridis, c. fetus, c. fetus subsp. fetus, c. fetus subsp. venerealis and c. hyointestinalis, and polymerase chain reaction. the partial sequence of dnas encoding the c ...19921307626
[result of the refinement of a technique for the isolation and identification of campylobacter from food commodities].campylobacter jejuni has been researched in raw milk, skin and excrement of chicken. all milk samples are negatives but 66% of chicken skin and 12% of chicken excrements are positives. an enrichment phase using brucella broth added with horse blood and antibiotics is necessary.19921309139
detection of ciprofloxacin resistance in gram-negative bacteria due to alterations in gyra.two plasmids containing the cloned escherichia coli wild-type gyra gene were used to transform ciprofloxacin-resistant gram-negative clinical isolates to screen for dna gyrase a-mediated quinolone resistance. the results show that the technique is simple and applicable to a wide range of gram-negative species including e. coli, enterobacter cloacae, klebsiella aerogenes, morganella morganii, proteus mirabilis, pseudomonas aeruginosa, campylobacter jejuni and neisseria gonorrhoeae. the use of an ...19921310671
genome maps of campylobacter jejuni and campylobacter coli.little information concerning the genome of either campylobacter jejuni or campylobacter coli is available. therefore, we constructed genomic maps of c. jejuni ua580 and c. coli ua417 by using pulsed-field gel electrophoresis. the genome sizes of c. jejuni and c. coli strains are approximately 1.7 mb, as determined by sali and smai digestion (n. chang and d. e. taylor, j. bacteriol. 172:5211-5217, 1990). the genomes of both species are represented by single circular dna molecules, and maps were ...19921313002
high-level quinolone resistance in clinical isolates of campylobacter jejuni.during a recent clinical trial of ciprofloxacin in the therapy of acute diarrhea, two subjects infected with campylobacter jejuni who received ciprofloxacin failed microbiologically and one also failed clinically. although both pretreatment isolates were susceptible to ciprofloxacin, the posttreatment isolates were resistant to ciprofloxacin (mic = 32 micrograms/ml) and to other quinolones. the posttreatment isolates remained susceptible to nonquinolone antimicrobials. dna gyrase holoenzyme was ...19921313069
expression of helicobacter pylori urease genes in escherichia coli grown under nitrogen-limiting conditions.helicobacter pylori produces a potent urease that is believed to play a role in the pathogenesis of gastroduodenal diseases. four genes (urea, ureb, urec, and ured) were previously shown to be able to achieve a urease-positive phenotype when introduced into campylobacter jejuni, whereas escherichia coli cells harboring these genes did not express urease activity (a. labigne, v. cussac, and p. courcoux, j. bacteriol. 173:1920-1931, 1991). results that demonstrate that h. pylori urease genes could ...19921313413
profiles of toxin production by thermophilic campylobacter of animal origin.seventy-five strains of campylobacter jejuni and c. coli, which were isolated from a variety of animal species, primarily poultry, were examined for production of toxin. polymyxin extracts were tested in in vitro assays using cho-ki, fcl (foetal calf lung), vero, hela and cef (chicken embryo fibroblast) cells. the toxic effects observed were cell rounding and death. extracts from almost all c. jejuni and c. coli strains were toxic to both cho-ki and fcl cells and 69.0% of c. jejuni isolates and ...19921313623
the fortuitous diagnosis of cholera in a two-year-old girl.we are reporting the fortuitous diagnosis of a case of cholera and the unusual failure of the commercial bacteriologic media that led to the unexpected isolation of vibrio cholerae. the case demonstrates the need for communication between the medical staff and laboratory personnel when an uncommon disease, such as cholera, is suspected. this case also alerts the clinician to the possibility of multiple enteric pathogens coinfecting a traveller.19921315232
microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics.a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ...19921315389
[neonatal campylobacter fetus septicemia: a case secondary to maternal-fetal contamination].a favourable outcome of a severe cambylobacter fetus sepsis in a neonate is reported. the transmission was probably vertical. his mother experienced diarrhoea 15 days before birth. no infecting organism was isolated from maternal stools, but maternal antibody response was significant. such a perinatal infection with meningitis and septicemia is uncommon. bacteriological diagnosis requires an enriched medium and a long incubation. the diagnosis should be evoked in view of an anamnestic context, a ...19921319039
in vitro activity of lomefloxacin and other antimicrobials against bacterial enteritis pathogens.lomefloxacin is a new, difluoroquinolone. in this study, the in vitro activity of lomefloxacin against clinical isolates of a variety of bacterial species associated with acute diarrheal disease was determined and compared with that of ciprofloxacin, ofloxacin, amoxicillin, sulphamethoxazole, trimethoprim, tetracycline, and chloramphenicol. bacterial isolates were obtained from different geographical areas, including western europe and the united kingdom, southern europe, africa, the middle east ...19921319301
campylobacter laridis colitis in a human immunodeficiency virus-positive patient treated with a quinolone. 19921319755
cloning of campylobacter jejuni genes required for leucine biosynthesis, and construction of leu-negative mutant of c. jejuni by shuttle transposon mutagenesis.campylobacter jejuni is a gram-negative pathogen responsible for diarrhoeal diseases in humans. to date, very little is known about the genetic organization and molecular biology of this microorganism. the cosmid vector phc79 was used to construct a genomic library from the total genomic dna of c. jejuni strain c31 in escherichia coli and recombinant cosmids capable of complementing the auxotrophic defect in leucine biosynthesis of e. coli hb101 were identified. three of 400 clones tested were f ...19921322552
differentiation between thermophilic campylobacter species by species-specific antibodies.the four species of thermophilic campylobacters, campylobacter jejuni, c. coli, c. upsaliensis and c. lari, are difficult to distinguish from each other because of their lack of reactivity in many conventional biochemical and physiological tests. those tests which do discriminate sometimes give discordant results. species-specific antibody preparations (aps), capable of discriminating between the thermophilic campylobacter species by dot-elisa, were raised by inoculation of mice with partially p ...19921322880
inflammatory-demyelinating polyneuropathies.recent advances in the pathogenesis and management of guillain-barré syndrome (gbs) and chronic inflammatory demyelinating polyneuropathy (cidp) are presented. these diseases are both diagnosable and treatable. the role of campylobacter infection preceding gbs and the use of intravenous immunoglobulin to treat cidp are discussed.19921323748
e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ...19921324301
synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens.our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ...19921324304
[microbial ecology of the digestive tract in patients with acute intestinal infections]. 19921324548
cytochrome composition and oxygen-dependent respiration-driven proton translocation in wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis.the membrane fractions of the microaerobically grown type strains of wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis contained membrane-bound cytochrome b, cytochrome c, and co-binding cytochrome c. soluble cytochrome c and co-binding cytochrome c were also present. although b. gracilis is oxidase negative, it possessed cytochrome c. with h2 or formate as the electron donor, proton efflux from anaerobic cells occurred upon addition of a pulse of oxygen. with f ...19921325865
[acute campylobacter jejuni enterocolitis resistant to quinolones in an hiv-positive patient]. 19921327169
comparative study of subgingival microbiological sampling techniques.the presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. the technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. in this study, paper point sampling and scaler sampling are compared. subgingival samples using both methods were taken from three surfaces in each of 12 patients at the following time points: at each of two appointments one week apart before treatment a ...19921328591
analysis of chromosome-sized dna from the bacterial genome of thermophilic campylobacter laridis by pulsed-field gel electrophoresis and physical mapping.three restriction enzymes apai, sali and smai, among nine enzymes tested, were found to produce distributions of dna fragments which were useful for analysis of chromosome-sized dna from thermophilic campylobacter laridis by pulsed-field gel electrophoresis. from experiments with c. laridis jcm2530t and four isolates of c. laridis, the size of the genome of c. laridis was calculated to range from 1,590 to 1,700 kb, with a mean of 1,640 kb. an smai restriction map was derived by the partial diges ...19921328825
[guillain-barré disease: a current treatment method using immunoglobulins]. 19921328903
in vitro and in vivo antibacterial activities of am-1155, a new 6-fluoro-8-methoxy quinolone.am-1155 is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and mycoplasma pneumoniae. am-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against staphylococcus aureus including methicillin-resistant strains, staphylococcus epidermidis, streptococcus pneumoniae, and enterococcus faecalis; its mics for 90% of strains tested were 0.10 to 0.78 micrograms/ml. the activity of am-1155 was comparable to that of ciprofloxacin ...19921332587
dna probe and enzyme analysis in periodontal diagnostics.recent research in periodontology has indicated that a finite number of bacterial species are associated with periodontal disease. this has generated the hypothesis that periodontal disease is essentially an infection due to one or more of the putative periodontopathogens; i.e., the specific plaque hypothesis (sph). this microbial specificity paradigm has already changed the way researchers and some clinicians view periodontal disease. the clinician must heed several cautions signs, however, if ...19921336048
immunological cross-reactivity between outer membrane pore proteins of campylobacter jejuni and escherichia coli.immunocrossreactivity between the major outer membrane protein (momp) of campylobacter jejuni 85h and the ompc porin of escherichia coli k-12 was observed. these results indicate that a common antigenic domain is conserved in both momp and ompc. this antigenic region is detected only after a 96 degrees c treatment suggesting that it is buried in the native conformation of the respective porins. in addition, differences were observed between the major outer membrane proteins from various c. jejun ...19921337053
clinical use of the new macrolides, azalides, and streptogramins in pediatrics.macrolides are the primary drugs of choice for a number of clinically significant infections in children. the clinical aspects of newer macrolides such as roxithromycin, clarithromycin, dirithromycin, flurithromycin, miocamycin, rokitamycin, azithromycin and rp 59500 are discussed in different pediatric infections including streptococcal infections (e.g. pharyngitis, otitis, pneumonia, skin infections), staphylococcus soft tissue infections, mycoplasma pneumonia, chlamydial infections as well as ...19921337554
the occurrence of high-level streptothricin resistance in thermotolerant campylobacters isolated from the slurry of swine and the environment.this is the first report on the occurrence of streptothricin resistance (mic > 400 micrograms/ml) in campylobacter spp. the majority of resistant strains has been typed as c. coli by biotyping and sds disc electrophoresis of bacterial whole cell proteins. the resistance to streptothricin was strongly connected with resistance to kanamycin (100%) and tetracycline (80%). as an important source of streptothricin-resistant campylobacter strains we localized slurry of swine previously fed with feed c ...19921338941
[salmonella m'bandaka isolated in a nursery].a epidemic of enteric pathogens bacteria occurred in december 1989 and caused two deaths in a nursery. there were many investigations between december 1989 and september 1990 trying to clarify this epidemic context. a new serovar of salmonella had for the first time been isolated from babies' feces in algeria. salmonella m'bandaka has been identified in more than 50% of analysis. during an hygienic investigation in this nursery the above salmonella m'bandaka was isolated in a open physiologic so ...19921339248
[preparation and use of fish fillet infusion as a basic medium for culturing bacteria].the authors present the first results on the utilization of fish infusion (ifp) as a basic medium for the cultivation of bacteria. the infusion was obtained from a common marine fish, corvina (micropogonias furnieri) according to the technique used in the preparation of beef infusion broth. streptococcus pyogenes, s. pneumoniae, neisseria meningitidis, campylobacter jejuni, escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, staphylococcus aureus and bacillus su ...19921345299
seropositivity for legionella in campylobacter infection. 19921346055
false-positive legionella serology in campylobacter infection. 19921346687
a review of hospitalized patients with bacterial gastroenteritis.the distribution and clinical management of thirty-two hospitalized patients with salmonella and campylobacter infections were reviewed and the impact of these infections on hospital resources was assessed. eighteen patients with salmonella infection had an age and sex distribution comparable with the community cases. in contrast, 10 out of 14 (71.4%) patients with campylobacter infection were under 20 years of age though the peak incidence of the infection in the community occurred in the 21-65 ...19921348754
physical map of campylobacter jejuni tgh9011 and localization of 10 genetic markers by use of pulsed-field gel electrophoresis.the physical map of campylobacter jejuni tgh9011 (atcc 43430) was constructed by mapping the three restriction enzyme sites sacii (ccgcgg), sali (gtcgac), and smai (cccggg) on the genome of c. jejuni by using pulsed-field gel electrophoresis and southern hybridization. a total of 25 restriction enzyme sites were mapped onto the c. jejuni chromosome. the size of the genome was reevaluated and was shown to be 1,812.5 kb. ten c. jejuni genetic markers that have been isolated in our laboratory were ...19921350583
crossreactions between legionella and campylobacter spp. 19921353168
emerging quinolone resistance in campylobacters. 19921353169
crossreactions between legionella and campylobacter spp. 19921354302
crossreactions between legionella and campylobacter spp. 19921354303
differentiation of the subspecies of campylobacter fetus by genomic sizing.campylobacter fetus subsp. fetus and c. fetus subsp. venerealis are currently differentiated by tolerance to glycine and by their epidemiology. analysis of c. fetus dna by pulsed-field gel electrophoresis, after digestion with the restriction endonucleases smai and sali, was used to differentiate between the subspecies. all strains presently identified as c. fetus subsp. fetus had a genomic size of 1.1 mb, whereas the majority of the c. fetus subsp. venerealis strains had a genomic size of 1.3 m ...19921354478
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