| protection activity of a novel probiotic strain of bacillus subtilis against salmonella enteritidis infection. | the activity of 240 bacterial isolates screened from the gastrointestinal tracts of native chickens were evaluated for use as a potential probiotic in food animal production in order to protect against animal diseases and reduce pathogenic contamination of human food products. in observing the antagonistic activity of 117 bacilli isolates, 10 of these isolates exhibited higher growth inhibition of seven foodborne pathogens, including salmonella enteritidis, salmonella typhimurium, escherichia co ... | 2011 | 21907374 |
| Inactivation of Listeria monocytogenes on agar and processed meat surfaces by atmospheric pressure plasma jets. | An apparatus for generating atmospheric pressure plasma (APP) jet was used to investigate the inactivation of Listeria monocytogenes on the surface of agar plates and slices of cooked chicken breast and ham. He, N2 (both 7 L/min), and mixtures of each with O2 (0.07 L/min) were used to produce the plasma jets. After treatment for 2 min with APP jets of He, He + O2, N2, or N2 + O2, the numbers of L. monocytogenes on agar plates were reduced by 0.87, 4.19, 4.26, and 7.59 log units, respectively. Si ... | 2011 | 21925030 |
| Effect of high pressure, in combination with antilisterial agents, on the growth of Listeria monocytogenes during extended storage of cooked chicken. | A cocktail of Listeria monocytogenes strains was inoculated into cooked chicken (~2.2 × 10³ CFU g?¹) which was then pressure-treated (600 MPa/2 min/20 °C) and stored for up to 105 days at 8 °C. In addition, sodium lactate (2% w/w) or a pressure-resistant Weissella viridescens strain, known to have antilisterial activity, were added to the meat prior to inoculation with the pathogen and pressure treatment, to investigate the effect on Listeria survival. Pressure treatment alone was not sufficient ... | 2011 | 21925036 |
| the effects of sodium lactate and starter cultures on ph, lactic acid bacteria, listeria monocytogenes and salmonella spp. levels in pure chicken dry fermented sausage. | two starter cultures (a and b) and seven sodium lactate concentrations were evaluated for chicken raw dry-fermented sausage processing. starter culture b contained more lactic acid bacteria and less staphylococci than starter a. their effects on acidification and inhibition of pathogens (listeria monocytogenes and salmonella spp.) were monitored. starter culture b grew faster and was less inhibited by sodium lactate, thus inducting a faster and more important ph drop into the sausages. with lowe ... | 2003 | 22063699 |
| control of listeria monocytogenes growth in a ready-to-eat poultry product using a bacteriophage. | a bacteriophage (phage) that infected strains of the species listeria monocytogenes as well as listeria ivanovii and listeria welshimeri, but not listeria grayi or listeria innocua, was isolated from sheep faeces. the phage had a contractile tail and an icosohedral head indicating that it was a myovirus, and was morphologically similar to phage a511. at 30 °c, phages added at 5.2 × 10⁷ pfu ml⁻¹ prevented the growth in broth of l. monocytogenes present at approximately twice this concentration fo ... | 2011 | 21925027 |
| effects of slightly acidic low concentration electrolyzed water on microbiological, physicochemical, and sensory quality of fresh chicken breast meat. | anticmicrobial effect of slightly acidic low concentration electrolyzed water (slalcew) and strong acidic electrolyzed water (staew) on fresh chicken breast meat was evaluated in this study. meat samples each of 10 ± 0.2 g in weight and 2.5 × 2.5 cm(2) in size were experimentally inoculated with listeria monocytogenes (atcc 19115) and salmonella typhimurium (atcc 14028) and subjected to dipping treatment (22 ± 2 °c for 10 min) with slalcew and staew. shelf-life study was conducted for inoculate ... | 2012 | 22260115 |
| the cultivation of the mushroom agaricus bisporus (champignon): micro-organisms and preservability. | cultivation of agaricus bisporus mushrooms requires the use of substrates that are potentially dangerous from the microbiological point of view, such as chicken and horse manure. microorganisms can pose risks to consumers and workers, and generate lower profits. packaging of fresh mushrooms with impermeable films is used to extend their shelf life but creates anaerobic and humidity conditions that could favour the growth of microorganisms such as listeria monocytogenes and clostridium botulinum. ... | 2011 | 22508614 |
| [outbreaks of salmonella spp., staphylococcus aureus and listeria monocytogenes associated with poultry consumption. systematic review]. | food borne diseases are a serious public health problem. poultry are often associated with these outbreaks. | 2012 | 23715186 |
| exploring the chicken embryo as a possible model for studying listeria monocytogenes pathogenicity. | listeria monocytogenes is a bacterial pathogen capable of causing severe infections in humans, often with fatal outcomes. many different animal models exist to study l. monocytogenes pathogenicity, and we have investigated the chicken embryo as an infection model: what are the benefits and possible drawbacks? we have compared a defined wild-type strain with its isogenic strains lacking well-characterized virulence factors. our results show that wild-type l. monocytogenes, already at a relatively ... | 2014 | 25540772 |
| age-associated alterations in cd8α+ dendritic cells impair cd8 t-cell expansion in response to an intracellular bacterium. | age-associated decline in immunity to infection has been documented across multiple pathogens, yet the relative contributions of the aged priming environment and of lymphocyte-intrinsic defects remain unclear. to address the impact of the aging environment on t-cell priming, adult naïve ot-i tcr transgenic cd8 t cells, specific for the h-2kb-restricted immunodominant ova(257-264) epitope, were transferred into adult or old recipient mice infected with the recombinant intracellular bacterium list ... | 2012 | 22862959 |
| proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules. | the chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. mass spectrometry analysis of the proteins recovered after heparin-affinity chromatography, revealed 20 proteins, including known antimicrobial proteins (avidin, lysozyme, tenp, ovalbu ... | 2016 | 27294500 |
| extracts containing clps of bacillus amyloliquefaciens jn68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant staphylococcus aureus and listeria monocytogenes. | bacillus amyloliquefaciens jn68, which has been discussed with regards to its antimicrobial activities, was successfully isolated from healthy chicken intestines in the present study. using the spot-on-the-lawn antagonism method, the preliminary study indicated that a suspension culture of the b. amyloliquefaciens jn68 strain can inhibit the growth of aspergillus niger and penicillium pinophilum. furthermore, the cyclic lipopeptides (clps) produced by the b. amyloliquefaciens jn68 strain were fu ... | 2016 | 27840979 |
| antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing bacillus subtilis kku213: potential as a probiotic strain. | the antimicrobial activity and probiotic properties of bacillus subtilis strain kku213, isolated from local soil, were investigated. the cell-free supernatant (cfs) of a kku213 culture containing crude bacteriocins exhibited inhibitory effects on gram-positive bacteria, including bacillus cereus, listeria monocytogenes, micrococcus luteus, and staphylococcus aureus. the antibacterial activity of the cfs precipitated with 40% ammonium sulfate (as) remained even after treatment at 60 and 100 °c, a ... | 2015 | 25440998 |
| antibacterial, antiviral, and antifungal properties of wines and winery byproducts in relation to their flavonoid content. | grapes produce organic compounds that may be involved in the defense of the plants against invading phytopathogens. these metabolites include numerous phenolic compounds that are also active against human pathogens. grapes are used to produce a variety of wines, grape juices, and raisins. grape pomace, seeds, and skins, the remains of the grapes that are a byproduct of winemaking, also contain numerous bioactive compounds that differ from those found in grapes and wines. this overview surveys an ... | 2014 | 24945318 |
| radiation-treated ready-to-eat (rte) chicken breast adobo for immuno-compromised patients. | usually in hospitals low-bacterial diets are served to immuno-compromised patients (icps). however, low-bacterial diets still pose a high risk of microbial infections and limit the food selection of the patients. thus, pathogen-free dishes must be made available. this study presents the development of pathogen-free ready-to-eat (rte) filipino ethnic food chicken breast adobo, sterilized by exposure to high-dose gamma rays (25 kgy) in combination with conventional treatments. frozen vacuum-packed ... | 2014 | 24912709 |
| food-borne pathogens of animal origin-diagnosis, prevention, control and their zoonotic significance: a review. | the term food borne diseases or food-borne illnesses or more commonly food poisoning are used to denote gastrointestinal complications that occur following recent consumption of a particular food or drink. millions of people suffer worldwide every year and the situation is quiet grave in developing nations creating social and economic strain. the food borne pathogens include various bacteria viz., salmonella, campylobacter, escherichia coli, listeria monocytogenes, yersinia enterocolitica, staph ... | 2013 | 24506006 |
| microbiological quality of chicken- and pork-based street-vended foods from taichung, taiwan, and laguna, philippines. | the microbiological quality of chicken- and pork-based street-food samples from taichung, taiwan's night markets (50) and laguna, philippines' public places (69) was evaluated in comparison to a microbiological guideline for ready-to-eat foods. different bacterial contamination patterns were observed between 'hot-grilled' and 'cold cooked/fried' food types from the two sampling locations with 'hot grilled' foods generally showing better microbiological quality. several samples were found to be u ... | 2013 | 23764220 |
| airborne microbial composition in a high-throughput poultry slaughtering facility. | a high-throughput chicken slaughtering facility in beijing was systematically investigated for numbers of airborne microorganisms. samples were assessed for counts of aerobic bacteria, staphylococcus aureus, total coliforms, escherichia coli, pseudomonas aeruginosa, listeria monocytogenes, bacillus cereus, and salmonella. during a 4-month period (september to december 2011), samples were collected for 10 min three times daily (preproduction, production, and postproduction). samples were collecte ... | 2013 | 23462077 |
| development of sandwich enzyme-linked immunosorbent assay for the detection of cronobacter muytjensii (formerly called enterobacter sakazakii). | this study aimed to produce a polyclonal antibody against cronobacter muytjensii (c. muytjensii, formerly called enterobacter sakazakii) and to develop an immunoassay for its detection. the optimum production of rabbit anti-c. muytjensii immunoglobulin g (igg) and chicken anti-c. muytjensii igy was reached in weeks 8 and 9, respectively. purification of rabbit anti-c. muytjensii igg from immunized rabbit sera was accomplished using the caprylic acid and ammonium sulfate precipitation method. as ... | 2012 | 22519814 |
| purification and characterization of a novel anti-campylobacter bacteriocin produced by lactobacillus curvatus dn317. | the lactic acid bacteria (lab) microbiota of saudi chicken ceca was determined. from 60 samples, 204 isolates of lactic acid bacteria were obtained. three isolates produced antimicrobial activities against campylobacter jejuni, listeria monocytogenes, and bacillus subtilis. the isolate dn317, which had the highest activity against campylobacter jejuni atcc 33560, was identified as lactobacillus curvatus (genbank accession numbers: kx353849 and kx353850). full inhibitory activity was observed aft ... | 2016 | 27812926 |
| purification of antilisterial peptide (subtilosin a) from novel bacillus tequilensis fr9 and demonstrate their pathogen invasion protection ability using human carcinoma cell line. | this study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (gallus gallus domesticus). fifty seven colonies were isolated and three isolates (fr4, fr9, and fr12) were selected and identified as lactobacillus gasseri fr4, bacillus tequilensis fr9, and l. animalis fr12 by 16s rrna sequencing. three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. especially, fr9 exh ... | 2016 | 27990138 |
| application of protective cultures against listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere. | to control the growth, or reduce the numbers, of food pathogens such as listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere (map), the effectiveness of protective cultures was evaluated in this study. leuconostoc pseudomesenteroides pck18 reduced the counts of l. monocytogenes by 1.22 log cfu/g in chicken burgers under map after 24 d. furthermore, a reduction of 1.16 log cfu/g in c. jejuni together with a delay in the growth of lactic acid bacte ... | 2013 | 23472035 |
| zoonotic public health hazards in backyard chickens. | backyard poultry has become increasingly popular in industrialized countries. in addition to keeping chickens for eggs and meat, owners often treat the birds as pets. however, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. the occurrence and the antimicrobial resistance of salmonella enterica, campylobacter spp., listeria monocytogenes and enteropathoge ... | 2016 | 26752227 |
| rapid identification and classification of campylobacter spp. using laser optical scattering technology. | campylobacter jejuni and campylobacter coli are the two important species responsible for most of the campylobacter infections in humans. reliable isolation and detection of campylobacter spp. from food samples are challenging due to the interferences from complex food substances and the fastidious growth requirements of this organism. in this study, a novel biosensor-based detection called bardot (bacterial rapid detection using optical scattering technology) was developed for high-throughput s ... | 2015 | 25583335 |
| efficacy of neutral electrolyzed water, quaternary ammonium and lactic acid-based solutions in controlling microbial contamination of food cutting boards using a manual spraying technique. | bactericidal activity of neutral electrolyzed water (new), quaternary ammonium (quat), and lactic acid-based solutions was investigated using a manual spraying technique against salmonella typhimurium, escherichia coli o157:h7, campylobacter jejuni, listeria monocytogenes and staphylococcus aureus that were inoculated onto the surface of scarred polypropylene and wooden food cutting boards. antimicrobial activity was also examined when using cutting boards in preparation of raw chopped beef, chi ... | 2016 | 27027449 |
| validation of a high-throughput immunobead array technique for multiplex detection of three foodborne pathogens in chicken products. | this study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, campylobacter jejuni, listeria monocytogenes, and salmonella spp., for its actual application in routine food testing. due to the limitation of the detection limit of the developed method, an enrichment step was included in this study by using campylobacter enrichment broth for c. jejuni and universal pre-enrichment broth for l. monocytogenes and salmonella ... | 2016 | 26950032 |
| effectiveness of inactivation of foodborne pathogens during simulated home pan frying of steak, hamburger or meat strips. | in order to evaluate the effect of simulated home pan frying of raw meat and meat preparations of different animal species on the thermal inactivation of pathogens, the heat resistance (d-value) of three strains of campylobacter jejuni, escherichia coli o157:h7, salmonella spp., listeria monocytogenes and two strains of generic e. coli was validated in bhi and adjusted bhi (i.e. ph5.6 and 1.5% nacl) at 60°c. the d-values were obtained of the linear phase of the survivor curves created in ginafit ... | 2015 | 26004267 |
| antibiotic susceptibility and prevalence of foodborne pathogens in poultry meat in romania. | the occurrence of pathogenic strains in poultry meat is of growing concern in romania. another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. this study aimed to evaluate the prevalence of pathogenic bacteria in poultry carcasses obtained in romania in 2012-2013 and to reveal the most prevalent patterns of antimicrobial resistance in the isolated strains. | 2015 | 25596569 |
| detection of salmonella spp. by a loop-mediated isothermal amplification (lamp) method targeting bcfd gene. | in this study, we developed and validated a loop-mediated isothermal amplification (lamp) assay for salmonella detection targeting bcfd gene, a conserved fimbrial operon gene existing in salmonella. the salmonella lamp assay we developed successfully amplified 44 salmonella strains (14 standard strains and 30 clinical isolates), but none of 9 non-salmonella standard strains (proteus mirabilis, listeria monocytogenes, escherichia coli, pseudomonas aeruginosa, shigella flexneri, shigella sonnei, k ... | 2014 | 25199410 |
| reduction of microbiological risk in minced meat by a combination of natural antimicrobials. | responsibility for food safety must be taken through the entire food-production chain, to avoid consumer cross-contamination. the antimicrobial activities of an alpinia katsumadai seed extract and epigallocatechin gallate (egcg), and their combination, were evaluated against individual food-borne pathogenic strains of listeria monocytogenes, escherichia coli and campylobacter jejuni, individually and as a cocktail, in chicken-meat juice and sterile minced meat as food models, and in minced meat ... | 2014 | 24532379 |
| behaviour of non-stressed and stressed listeria monocytogenes and campylobacter jejuni cells on fresh chicken burger meat packaged under modified atmosphere and inoculated with protective culture. | numerous investigations have provided evidence that chicken products are a source of listeria monocytogenes and campylobacter jejuni. different strategies applied in final products are needed to prevent consumers' contamination. in this work, the combination of modified atmosphere packaging (map) and protective culture to control the growth of freeze stressed and non-stressed l. monocytogenes and c. jejuni on fresh chicken meat burger was studied. meat burgers were inoculated with l. monocytogen ... | 2012 | 22831819 |
| campylobacter, salmonella, listeria monocytogenes, verotoxigenic escherichia coli, and escherichia coli prevalence, enumeration, and subtypes on retail chicken breasts with and without skin. | this study examined the prevalence, counts, and subtypes of campylobacter, salmonella, listeria monocytogenes, verotoxigenic escherichia coli (vtec), and e. coli on raw retail chicken breast with the skin on versus the skin off. from january to december 2007, 187 raw skin-on chicken breasts and 131 skin-off chicken breasts were collected from randomly selected retail grocery stores in the region of waterloo, ontario, canada. campylobacter isolates were recovered from a higher proportion of the s ... | 2012 | 22221353 |
| inactivation of salmonella, listeria monocytogenes and enterococcus faecium nrrl b-2354 in a selection of low moisture foods. | the aims of this study were to obtain data on survival and heat resistance of cocktails of salmonella, listeria monocytogenes and the surrogate enterococcus faecium (nrrl b-2354) in four low moisture foods (confectionery formulation, chicken meat powder, pet food and savoury seasoning) during storage before processing. inoculated samples were stored at 16°c and cell viability examined at day 0, 3, 7 and 21. at each time point, the heat resistance at 80°c was determined. the purpose was to determ ... | 2016 | 27174678 |
| enrichment, amplification, and sequence-based typing of salmonella enterica and other foodborne pathogens. | detection of salmonella enterica in foods typically involves microbiological enrichment, molecular-based assay, and subsequent isolation and identification of a pure culture. this is ideally followed by strain typing, which provides information critical to the investigation of outbreaks and the attribution of their sources. pulsed-field gel electrophoresis is the "gold standard" for s. enterica strain typing, but its limitations have encouraged the search for alternative methods, including whole ... | 2017 | 28221883 |
| inactivation of salmonella spp., pathogenic escherichia coli, staphylococcus spp., or listeria monocytogenes in chicken purge or skin using a 405-nm led array. | raw poultry are sometimes contaminated with foodborne pathogens, which can lead to illness in humans. in recent years research has focused on a variety of light technologies to decontaminate food and food contact surfaces during meat and poultry processing. in this study we evaluated the ability of 405-nm light generated from an led array to inactivate multi-isolate cocktails of either salmonella spp., pathogenic escherichia coli, staphylococcus spp., or listeria monocytogenes suspended in chick ... | 2017 | 28213017 |
| modeling optimal process conditions for uv-heat inactivation of foodborne pathogens in liquid foods. | the combination of ultraviolet radiation and heat (uv-h treatment) has been demonstrated as a promising strategy to overcome the limited uv germicidal effect in fruit juices. nonetheless, there are so far no data regarding the efficacy of the combined process for the inactivation of bacterial foodborne pathogens in other liquid foods with different ph and composition. in this investigation, the optimum uv-h processing conditions for the inactivation of escherichia coli, salmonella typhimurium, l ... | 2016 | 27554141 |
| foodborne bacterial pathogens in retail prepacked ready-to-eat mixed ingredient salads. | prepacked ready-to-eat mixed ingredient salads (rte salads) are readily available whole meals that include a variety of ingredients such as raw vegetables, cooked meat, and pasta. as part of a trend toward healthy convenience foods, rte salads have become an increasingly popular product among consumers. however, data on the incidence of foodborne pathogens in rte salads are scarce. in this study, the microbiological safety of 141 rte salads containing chicken, ham, or smoked salmon was investiga ... | 2016 | 27296602 |
| uv-heat treatments for the control of foodborne microbial pathogens in chicken broth. | this investigation established the process criteria for using uv-c light and mild heat (uv-h treatment) to inactivate 5-log10 cycles (performance criterion) of common foodborne pathogen populations, escherichia coli, salmonella typhimurium, listeria monocytogenes, and staphylococcus aureus, when inoculated in chicken broth. to define the target microorganism and the proper uv-h treatment conditions (including uv dose, treatment time, and temperature) that would achieve the stated performance cri ... | 2015 | 26539493 |
| detection of poultry meat specific bacteria using ftir spectroscopy and chemometrics. | ftir spectra of poultry meat specific bacteria viz. salmonella enteritidis, pseudomonas ludensis, listeria monocytogenes and escherichia coli were collected and investigated for identification of spectral windows capable of bacterial classification and quantification. two separate datasets obtained at different times were used in the study to check reproducibility of results. multivariate data analysis techniques viz. principal component analysis (pca), partial least-squares discriminant analysi ... | 2015 | 26028771 |
| passive maternal exposure to environmental microbes selectively modulates the innate defences of chicken egg white by increasing some of its antibacterial activities. | egg defence against bacterial contamination relies on immunoglobulins (igy) concentrated in the yolk and antimicrobial peptides/proteins predominantly localized in the egg white (ew). hens contaminated with pathogenic microorganisms export specific igys to the egg (adaptative immunity). no evidence of such regulation has been reported for the antimicrobial peptides/proteins (innate immunity) which are preventively secreted by the hen oviduct and are active against a large range of microbes. we i ... | 2013 | 23758641 |
| development of a dna macroarray for simultaneous detection of multiple foodborne pathogenic bacteria in fresh chicken meat. | a dna macroarray was developed to provide the ability to detect multiple foodborne pathogens in fresh chicken meat. probes targeted to the 16s rrna and genus- and species-specific genes, including fimy, ipah, prfa, and uspa, were selected for the specific detection of salmonella spp., shigella spp., listeria monocytogenes, and escherichia coli, respectively. the combination of target gene amplification by pcr and a dna macroarray in our system was able to distinguish all target bacteria from pur ... | 2013 | 23754709 |
| behaviour of co-inoculated pathogenic and spoilage bacteria on poultry following several decontamination treatments. | the potential of chemical decontaminants to cause harmful effects on human health is among the causes of the rejection of antimicrobial treatments for removing surface contamination from poultry carcasses in the european union. this study was undertaken to determine whether decontaminants might give a competitive advantage to pathogenic bacteria on poultry and involve a potential risk to consumer. a total of 144 chicken legs were co-inoculated with similar concentrations of pathogenic bacteria ( ... | 2012 | 23072701 |
| efficient role of bactn635 on the safety properties, sensory attributes, and texture profile of raw minced meat beef and chicken breast. | bacteriocin bactn635, produced by lactobacillus plantarum sp. tn635, was purified and characterised in previous work. in this study we report the biotechnological application of this bacteriocin as a biopreservative during storage at 4°c of raw minced meat beef and chicken breast. overall, the results obtained showed that the addition of the semi-purified bactn635 at 500 or 1000 au g(-1) in raw minced meat beef and chicken breast can delay the proliferation of spoilage microorganisms, suppress t ... | 2014 | 24320082 |
| the antimicrobial action of resveratrol against listeria monocytogenes in food-based models and its antibiofilm properties. | resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a natural phytoalexin synthesized by plants in response to stress. this compound has several beneficial documented properties, namely anti-inflammatory, antioxidant, neuroprotective and antimicrobial activities. in this study the antimicrobial activity of resveratrol against listeria monocytogenes and listeria innocua was investigated. | 2016 | 26867522 |
| prevalence and antimicrobial resistance of listeria species isolated from different types of raw meat in iran. | listeria and particularly listeria monocytogenes are important foodborne pathogens that can cause listeriosis and severe complications in immunocompromised individuals, children, pregnant women, and the elderly. the objective of this study was to determine the prevalence of listeria spp. in raw meat in iran. from july 2010 to november 2011, a total of 1,107 samples of various raw meats were obtained from randomly selected retail butcher shops. the results of conventional bacteriologic and pcr me ... | 2012 | 23212021 |
| contamination by salmonella spp., campylobacter spp. and listeria spp. of most popular chicken- and pork-sausages sold in reunion island. | one of the most popular meat products of the local "cuisine" is sausage composed with 100% chicken or 100% pork. in this study, we aimed to determine the presence of salmonella spp., campylobacter spp. and listeria spp. in chicken- and pork-sausages, quantify salmonella spp. population and identify the factors that could be associated with contamination in the outlets. two hundred and three batches of pork and chicken sausages were randomly collected from 67 local outlets (supermarkets, grocerie ... | 2017 | 28371717 |
| fate of listeria monocytogenes , pathogenic yersinia enterocolitica , and escherichia coli o157:h7 gfp(+) in ready-to-eat salad during cold storage: what is the risk to consumers? | in this study, we investigated the fate of listeria monocytogenes , pathogenic yersinia enterocolitica , and escherichia coli o157:h7 gfp(+) inoculated in low numbers into ready-to-eat baby spinach and mixed-ingredient salad (baby spinach with chicken meat). samples were stored at recommended maximum refrigerator temperature (8°c in sweden) or at an abuse temperature (15°c) for up to 7 days. mixed-ingredient salad supported considerable growth when stored at 15°c during shelf life (3 days), with ... | 2017 | 28221975 |
| nanoengineered eggshell-silver tailored copolyester polymer blend film with antimicrobial properties. | in this study, the reinforcement effect of different proportions of eggshell/silver (es-ag) nanomaterial on the structural and antimicrobial properties of 70/30 poly(butylene-co-adipate terephthalate)/polylactic acid (pbat/pla) immiscible blends was investigated. the es-ag was synthesized using a single step ball milling process and characterized with x-ray diffraction (xrd), x-ray photoelectron spectroscopy (xps), and transmission electron microscopy (tem). these results confirmed the existence ... | 2017 | 28206760 |
| use of rhodomyrtus tomentosa ethanolic leaf extract for the bio-control of listeria monocytogenes post-cooking contamination in cooked chicken meat. | controlling foodborne pathogen in ready-to-eat food is important in food safety. the present study accessed the potential use of rhodomyrtus tomentosa ethanolic leaf extract as a bio-control agent against listeria monocytogenes in cooked chicken meat model system. the antilisterial activity of the plant extract was better under microwave condition and enhanced as storage temperature increased from 4 to 37 °c. the extract could reduce l. monocytogenes numbers at low (10(4) cfu/g) and high (10(6) ... | 2016 | 28115764 |
| evaluation of the 3m™ molecular detection assay (mda) 2 - listeria monocytogenes for the detection of listeria monocytogenes in a variety of foods and select environmental surfaces: collaborative study, first action 2016.08. | the 3m™ molecular detection assay (mda) 2 - listeria monocytogenes uses loop-mediated isothermal amplification of unique dna target sequences combined with bioluminescence to rapidly detect listeria monocytogenes in a broad range of food types and on environmental surfaces. using an unpaired study design, technicians from 13 laboratories located in the united states and canada compared the 3m mda 2 - listeria monocytogenes to the u.s. department of agriculture food safety inspection service micr ... | 2017 | 28059049 |
| prevalence and antibiotic susceptibility profiles of listeria monocytogenes contamination of chicken flocks and meat in oyo state, south-western nigeria: public health implications. | food contamination with listeria monocytogenes is on the increase posing threats to public health with growing trends in food products recalls due to suspected listeria contamination. | 2016 | 27980380 |
| effect of sodium alginate coating incorporated with nisin, cinnamomum zeylanicum, and rosemary essential oils on microbial quality of chicken meat and fate of listeria monocytogenes during refrigeration. | the present study was conducted to preserve the microbial quality of chicken meat fillets during storage time by using sodium alginate active coating solutions incorporated with different natural antimicrobials including nisin, cinnamomum zeylanicum (cinnamon), and rosemary essential oils (eos) which were added individually and in combination. the samples were stored in refrigeration condition for 15days and were analyzed for total viable count, enterobacteriaceae count, lactic acid bacteria cou ... | 2016 | 27620825 |
| listeria monocytogenes infection of hd11, chicken macrophage-like cells. | listeria monocytogenes can be carried by and infect poultry, although the clinical disease in birds is rare. escape from macrophage phagocytosis is a key step in pathogenesis for l. monocytogenes therefore, we investigated the infection of the chicken macrophage-like cell line hd11 with 2 strains of l. monocytogenes egd-e and scott a. after infection, l. monocytogenes was quantified by spread plating and hd11 was quantified with trypan blue exclusion stain before enumeration. the standard macrop ... | 2016 | 27738121 |
| the development of methods for the detection of salmonella in chickens by a combination of immunomagnetic separation and pcrs. | micro- and nanoimmunomagnetic beads (mimbs and nimbs) used for immunomagnetic separation (ims) with pcr were studied for the rapid detection of salmonella. the capture efficiency of the two different imbs was evaluated by a conventional plate counting method, and the binding pattern was studied using scanning electron microscopy. the specificity of the imbs was tested with salmonella, shigella flexneri, enterohemorrhagic escherichia coli o157:h7, and listeria monocytogenes. by comparing the pre- ... | 2016 | 27730684 |
| a chitosan-coated liposome encapsulating antibacterial peptide, apep10: characterisation, triggered-release effects and antilisterial activity in thaw water of frozen chicken. | contamination of listeria monocytogenes in food and their processing environment is a focus of attention in the food industry. to achieve the controlled release of antibacterial agents to a food processing environment contaminated by l. monocytogenes, chitosan-stabilised liposomes encapsulating apep10 (glarclagtl), an antibacterial peptide derived from boiled-dried anchovies, were prepared by utilising listeria toxins to activate the peptide release. characteristics including the particle size, ... | 2016 | 27713959 |
| anti-listerial effect of selected essential oils and thymol. | the anti-listerial effect of marjoram, thyme essential oils (eos) and thymol on listeria monocytogenes inoculated chicken breast fillets was investigated. before inoculation the fillets were pretreated by washing or not under running tap water. inoculated samples were kept at 6 °c for 24 h to allow the growth of l. monocytogenes. after this, the fillets were put in marinating solutions containing salt (5%) and eos or thymol in mic/2 concentration established in vitro. total germ count (tgc) and ... | 2016 | 27630055 |
| inhibition of listeria monocytogenes on cooked cured chicken breasts by acidified coating containing allyl isothiocyanate or deodorized oriental mustard extract. | ready-to-eat meats are considered foods at high risk to cause life-threatening listeria monocytogenes infections. this study screened 5 l. monocytogenes strains for their ability to hydrolyze sinigrin (a glucosinolate in oriental mustard), which formed allyl isothiocyanate (aitc) and reduced l. monocytogenes viability on inoculated vacuum-packed, cooked, cured roast chicken slices at 4 °c. tests involved incorporation of 25-50 μl/g aitc directly or 100-250 mg/g oriental mustard extract in 0.5% ( ... | 2016 | 27052706 |
| effect of salt types and concentrations on the high-pressure inactivation of listeria monocytogenes in ground chicken. | national and international health agencies have recommended a significant reduction in daily intake of sodium by reducing the amount of nacl in foods, specifically processed meats. however, sodium reduction could increase the risk of survival and growth of spoilage and pathogenic microorganisms on these products. therefore, alternate processing technologies to improve safety of sodium reduced foods are necessary. this study examined the effects of three different salt types and concentrations on ... | 2016 | 26613161 |
| in-vitro antioxidant and antibacterial properties of fermentatively and enzymatically prepared chicken liver protein hydrolysates. | protein hydrolysates were prepared from chicken liver using fermentation and enzymatic hydrolysis. the lactic acid bacteria pediococcus acidilactici ncim5368 was employed in the fermentation process and a commercial protease (alcalase® 2.5) was used in enzymatic hydrolysis. chicken liver hydrolysates prepared by fermentation (fclh) and enzymatic hydrolysis (eclh) revealed appreciable amounts of protein [55.85 and 61.34 %; on dry weight basis, respectively]. fermentation and enzymatic hydrolysis ... | 2015 | 26604378 |
| a comparison analysis of listeria monocytogenes isolates recovered from chicken carcasses and human by using rapd pcr. | chicken meat is a valuable source of protein and consumption of it continues to rise day to day. the aim of the present study was to evaluate the genetic homogeneity of listeria monocytogenes (l. monocytogenes) isolates obtained from chicken carcasses and human. random amplification of polymorphic dna (rapd) pcr with three different primers were used to analyze the 31 l. monocytogenes isolates recovered from human and chicken carcasses. primers were d8635, hlwl74, and opm01. scanned images of ra ... | 2015 | 26309714 |
| sonorensin: a new bacteriocin with potential of an anti-biofilm agent and a food biopreservative. | the emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. in this study, sonorensin, predicted to belong to ... | 2015 | 26292786 |
| the effect of short-time microwave exposures on listeria monocytogenes inoculated onto chicken meat portions. | listeria monocytogenes can be found throughout the environment and in many foods. it is associated primarily with meat and animal products. listeria monocytogenes has become increasingly important as a food-borne pathogen. the aim of this study was to evaluate the effect of microwave (mw) treatment of chicken meat samples which were inoculated with l. monocytogenes. drumettes of broiler carcasses were soaked in fully growth of l. monocytogenes in brain-heart infusion broth. the swab samples were ... | 2015 | 26261715 |
| characterization of specific alleles in inla and prfa of listeria monocytogenes isolated from foods in osaka, japan and their ability to invade caco-2 cells. | listeria monocytogenes expresses the surface protein internalin a (inla), enabling the invasion of human intestinal epithelial cells to cause severe food-borne diseases. full-length sequence analysis of inla of 114 food isolates resulted in the detection of 29 isolates with a premature stop codon (pmsc) mutation and 6 isolates with 3-codon deletion mutations (aa 738 to 740) in inla. the isolates with inla pmscs demonstrated a significantly lower level of invasion than the other food isolates in ... | 2015 | 26143289 |
| using the chicken embryo to assess virulence of listeria monocytogenes and to model other microbial infections. | microbial infections are a global health problem, particularly as microbes are continually developing resistance to antimicrobial treatments. an effective and reliable method for testing the virulence of different microbial pathogens is therefore a useful research tool. this protocol describes how the chicken embryo can be used as a trustworthy, inexpensive, ethically desirable and quickly accessible model to assess the virulence of the human bacterial pathogen listeria monocytogenes, which can ... | 2015 | 26134955 |
| atlas(®) listeria monocytogenes lmg2 detection assay using transcription mediated amplification to detect listeria monocytogenes in selected foods and stainless steel surface. | the atlas listeria monocytogenes lmg2 detection assay, developed by roka bioscience inc., was compared to a reference culture method for seven food types (hot dogs, cured ham, deli turkey, chicken salad, vanilla ice cream, frozen chocolate cream pie, and frozen cheese pizza) and one surface (stainless steel, grade 316). a 125 g portion of deli turkey was tested using a 1:4 food:media dilution ratio, and a 25 g portion for all other foods was tested using 1:9 food:media dilution ratio. the enrich ... | 2016 | 25902984 |
| evaluation of novel micronized encapsulated essential oil-containing phosphate and lactate blends for growth inhibition of listeria monocytogenes and salmonella on poultry bologna, pork ham, and roast beef ready-to-eat deli loaves. | essential oils and their constituents are reported to possess potent antimicrobial activity, but their use in food processing is limited because of low solubility in aqueous systems and volatilization during processing. two proprietary noncommercial essential oil-containing phosphate blends were evaluated for antimicrobial activity against salmonella enterica cocktail (sc)-and listeria monocytogenes (lm)-inoculated deli meat products made from pork, poultry, or beef. four treatments were tested ... | 2015 | 25836394 |
| combined effect of a nanoemulsion of d-limonene and nisin on listeria monocytogenes growth and viability in culture media and foods. | the present work evaluated the antibacterial effect of nanoemulsions from natural compounds d-limonene and nisin against listeria monocytogenes in tryptic soy broth growth medium, chicken broth, and vegetable cream. experiments were performed by means of optical density growth curves and studies of viability in culture media and foods. stability of nanoemulsions was evaluated by a mastersizer 2000 equipment. results showed greater effectiveness when applying d-limonene in form of nanoemulsion th ... | 2016 | 25788168 |
| effect of chitosan on spoilage bacteria, escherichia coli and listeria monocytogenes in cured chicken meat. | the effects of essential oil (eo) of oregano and chitosan on the microbial quality and growth inhibition of listeria monocytogenes and escherichia coli o157:h7 in the iranian traditional ready-to-barbecue chicken was evaluated. thus, three groups of samples were prepared. one of them was considered to evaluate for aerobic plate count (apc), lactic acid bacteria (lab), psychrophilic and enterobacteriacae counts and the other two groups were inoculated with e. coli o157:h7 and l. monocytogenes 4b ... | 2015 | 25735728 |
| manure source and age affect survival of zoonotic pathogens during aerobic composting at sublethal temperatures. | heat is the primary mechanism by which aerobic composting inactivates zoonotic bacterial pathogens residing within animal manures, but at sublethal temperatures, the time necessary to hold the compost materials to ensure pathogen inactivation is uncertain. to determine the influence of the type of nitrogen amendment on inactivation of salmonella, listeria monocytogenes, and escherichia coli o157:h7 in compost mixtures stored at sublethal temperatures, specific variables investigated in these stu ... | 2015 | 25710145 |
| essential oils from herbs against foodborne pathogens in chicken sausage. | consumption of chicken meat and its products, especially sausage, have increased in recent years. however, this product is susceptible to microbial contamination during manufacturing, which compromises its shelf life. the flavoring and preservative activities of essential oils (eo) have been recognized and the application of these antimicrobial agents as natural active compounds in food preservation has shown promise. the aim of this study was to evaluate the effect of ocimum basilicum and origa ... | 2015 | 25492235 |
| environmental and biological factors influencing the uv-c resistance of listeria monocytogenes. | in this investigation, the effect of microbiological factors (strain, growth phase, exposition to sublethal stresses, and photorepair ability), treatment medium characteristics (ph, water activity, and absorption coefficient), and processing parameters (dose and temperature) on the uv resistance of listeria monocytogenes was studied. the dose to inactivate 99.99% of the initial population of the five strains tested ranged from 21.84 j/ml (stcc 5672) to 14.66 j/ml (stcc 4031). the uv inactivation ... | 2015 | 25475293 |
| prevalence and populations of listeria monocytogenes in meat products retailed in sao paulo, brazil. | this study evaluated the prevalence of the populations and serotypes of listeria monocytogenes in 552 refrigerated samples of ground beef, chicken leg, hot dog, and pork sausage collected in supermarkets in the city of sao paulo, sp, brazil, between may 2008 and july 2009. the supermarkets were selected after stratification by geographical region and by random draw. tests for presence and enumeration of l. monocytogenes were based on iso 11290-1:1996/amd.1:2004 and iso 11290-2:1998 methods, resp ... | 2014 | 25407460 |
| efficacy of acetic acid against listeria monocytogenes attached to poultry skin during refrigerated storage. | this work evaluates the effect of acetic acid dipping on the growth of l. monocytogenes on poultry legs stored at 4 °c for eight days. fresh inoculated chicken legs were dipped into either a 1% or 2% acetic acid solution (v/v) or distilled water (control). changes in mesophiles, psychrotrophs, enterobacteriaceae counts and sensorial characteristics (odor, color, texture and overall appearance) were also evaluated. the shelf life of the samples washed with acetic acid was extended by at least two ... | 2014 | 28234335 |
| thermal and nonthermal factors affecting survival of salmonella and listeria monocytogenes in animal manure-based compost mixtures. | reduction of enteric pathogens in animal manures before field application is essential for mitigating the risk of foodborne illness associated with produce. aerobic composting of manures has been advocated as an effective treatment for reducing pathogen populations, and heat is a major factor contributing to pathogen inactivation. this study was initiated to determine the potential contribution of both thermal and nonthermal (ph, volatile acids, and ammonia) factors to pathogen inactivation duri ... | 2014 | 25198842 |
| prevalence and antimicrobial susceptibility of listeria monocytogenes on chicken carcasses in bandung, indonesia. | this study was conducted to determine the prevalence and quantify the number of listeria monocytogenes in fresh chicken carcasses sold in traditional markets and supermarkets in bandung, west java, indonesia, and to determine the antimicrobial resistance patterns of the isolated l. monocytogenes strains. the overall prevalence of l. monocytogenes in chicken carcasses was 15.8% (29/184). when comparing samples from traditional markets and supermarkets, no significant difference in the l. monocyto ... | 2014 | 25198605 |
| combined effects of chlorine and thiamine dilauryl sulfate on reduction of listeria monocytogenes in chicken breast and development of predictive growth models. | the inhibitory effect of chlorine (50, 100, and 200 ml/kg) and thiamine dilauryl sulfate (tds: 100, 500, and 1,000 mg/kg) on listeria monocytogenes in chicken breast was investigated. also, predictive growth models as a function of chlorine and tds concentration, and storage temperature (4, 10, and 15°c) were developed using a polynomial model. listeria monocytogenes counts were significantly (p < 0.05) different in samples treated with sterile distilled water and combinations of chlorine and td ... | 2014 | 24879700 |
| technologies and mechanisms for safety control of ready-to-eat muscle foods: an updated review. | ready-to-eat (rte) muscle foods refer to a general category of meat and poultry products that are fully cooked and consumable without reheating. these products, including whole and sliced pork, beef, turkey, chicken, and variety of meats, in the forms of ham, roast, rolls, sausage, and frankfurter, are widely available in the delicatessen section of retail stores or various food service outlets. however, difficulties in avoidance of contamination by foodborne pathogens, notably listeria monocyto ... | 2015 | 24754253 |
| antibacterial activity of bacteriocin-like substance p34 on listeria monocytogenes in chicken sausage. | the antimicrobial activity of the bacteriocin-like substance (bls) p34 against listeria monocytogenes was investigated in chicken sausage. the bls was applied to chicken sausages (256 au g(-1)) previously inoculated with a suspension of 10(2) cfu g(-1) of l. monocytogenes. bls p34 inhibited the indicator microorganism in situ in all incubation times for up to 10 days at 5 °c. the effectiveness of bls p34 was increased when it was added in combination with nisin. the bacteriocin was also tested i ... | 2013 | 24688506 |
| in-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. | consumption of foods contaminated with pathogenic bacteria is a major public health concern. foods contain microorganisms, the overwhelming majority of which are nonpathogenic, some are responsible for food spoilage, and some cause serious illness leading to death or a variety of diseases in humans. the key challenge in food safety is to rapidly screen foods to determine the presence of pathogens so that appropriate intervention protocols can be pursued. a simple fluorometric immunological metho ... | 2014 | 24583684 |
| inhibitory effect of chlorine and ultraviolet radiation on growth of listeria monocytogenes in chicken breast and development of predictive growth models. | the inhibitory effect of chlorine (50, 100, and 200 mg/kg) was investigated with and without uv radiation (300 mw·s/cm(2)) for the growth of listeria monocytogenes in chicken breast meat. using a polynomial model, predictive growth models were also developed as a function of chlorine concentration, uv exposure, and storage temperature (4, 10, and 15°c). a maximum l. monocytogenes reduction (0.8 log cfu, cfu/g) was obtained when combining chlorine at 200 mg/kg and uv at 300 mw·s/cm(2), and a maxi ... | 2014 | 24570440 |
| in vitro detection of pathogenic listeria monocytogenes from food sources by conventional, molecular and cell culture method. | among current in vitro methods for identification of pathogenic listeria monocytogenes (l. monocytogenes) rely on growth in culture media, followed by isolation, and biochemical and serological identification. now pcr (polymerase chain reaction) has been used for the rapid, sensitive and specific detection of pathogenic l. monocytogenes. the pathogenicity of the organism is highly correlated with haemolytic factor known as listeriolysin o (llo). a total of 400 samples from meat and 250 samples f ... | 2013 | 24516442 |
| listeria monocytogenes infection in poultry and its public health importance with special reference to food borne zoonoses. | listeriosis is a disease that causes septicemia or encephalitis in humans, animals and birds. although, the disease is rare and sporadic in poultry but if occurs then causes septicemia or sometimes localized encephalitis. occasionally, the disease is seen in young chicks and the causative agent, like in humans and animals, is listeria monocytogenes. the organism is capable to infect almost all animals and poultry; however, outbreaks of listeriosis are infrequent in birds. it is widely distribute ... | 2013 | 24498796 |
| listeria monocytogenes in retailed raw chicken meat in turkey. | the objectives of this study were, to find the prevalence and antimicrobial resistance of l. monocytogenes from a total of 116 chicken meat samples including 50 carcasses and 66 meat parts marketed in turkey between 2008 and 2009 using immunomagnetic separation (ims) based cultivation technique, to detect the hlya gene for the verification of the isolates by pcr, and to identify the genoserotypes of the l. monocytogenes isolates by multiplex pcr assay. in the study, 51 l. monocytogenes colonies ... | 2014 | 24490342 |
| biofilm formation under different temperature conditions by a single genotype of persistent listeria monocytogenes strains. | some listeria monocytogenes strains, termed persistent strains, originate from the same processing plant and have the ability to survive and grow over extended periods of time at contamination sources. in order to evaluate biofilm formation by such persistent strains, we isolated the pathogen from chicken samples collected from the same retail shop in repeated visits over 6 months. strains that were of serotype 1/2b and were assigned to the same genotype by multi-virulence-locus sequence typing ... | 2014 | 24406011 |
| prevalence of salmonella serovars, listeria monocytogenes, and escherichia coli o157:h7 in mediterranean ready-to-eat meat products in jordan. | the presence of salmonella, listeria monocytogenes, and escherichia coli o157:h7 in ready-to-eat (rte) meat products is considered a major concern for food control authorities worldwide. the aims of this study were to determine (i) the prevalence of salmonella, l. monocytogenes, and e. coli o157:h7 in mediterranean rte chicken and beef (cb) products sold in jordanian restaurants and (ii) the susceptibility of the isolates to antibiotics. a total of 1,028 samples of various types of rte cb produc ... | 2014 | 24406006 |
| crystal structure of the interferon gamma receptor alpha chain from chicken reveals an undetected extra helix compared with the human counterparts. | interferon gamma (ifn-γ) is an important cytokine that induces antiviral, antiproliferative, and immunomodulatory effects on target cells, and is also crucial in the early defense against intracellular parasites, such as listeria monocytogenes and toxoplasma gondii. the biological activity of ifn-γ relies upon the formation of a complex with its 2 receptors, the interferon gamma alpha chain (ifngr1) and beta chain (ifngr2), which are type ii cytokine receptors. structural models of ligand-recept ... | 2014 | 24283193 |
| use of germicidal uv light to reduce low numbers of listeria monocytogenes on raw chicken meat. | listeria monocytogenes is a common constituent of the microbiological community in poultry processing plants and can be found in low numbers on raw poultry. raw meat is the most important source of this pathogen in commercial cooking facilities. germicidal uv light was tested as a means to kill l. monocytogenes inoculated onto broiler breast fillets. treatments at 800 μw/ cm(2) for 5 s to 5 min of exposure were tested against inocula of 35 to 60 cells per fillet. all fillets were sampled by rins ... | 2013 | 24215703 |
| detection of listeria monocytogenes from selective enrichment broth using maldi-tof mass spectrometry. | conventional methods used for primary detection of listeria monocytogenes from foods and subsequent confirmation of presumptive positive samples involve prolonged incubation and biochemical testing which generally require four to five days to obtain a result. in the current study, a simple and rapid proteomics-based maldi-tof ms approach was developed to detect l. monocytogenes directly from selective enrichment broths. milk samples spiked with single species and multiple species cultures were i ... | 2014 | 24080423 |
| contamination of poultry products with listeria monocytogenes at poultry processing plants. | this study aimed to confirm that poultry products packed at poultry processing plants have already been contaminated with listeria monocytogenes and that poultry products contaminated with l. monocytogenes are derived from broiler flocks infected with l. monocytogenes. l. monocytogenes was isolated from 16.8% (58/345) of chicken breast products and 2.3% (8/345) of chicken liver products. in contrast, l. monocytogenes was isolated from the pooled cecal content sample from only 1 (4%) of 25 flocks ... | 2014 | 24018827 |
| effect of different concentrations of acetic, citric, and propionic acid dipping solutions on bacterial contamination of raw chicken skin. | bacterial contamination of raw, processed poultry may include spoilage bacteria and foodborne pathogens. we evaluated different combinations of organic acid (oa) wash solutions for their ability to reduce bacterial contamination of raw chicken skin and to inhibit growth of spoilage bacteria and pathogens on skin during refrigerated storage. in experiment 1, raw chicken skin samples were dipped into a suspension of either 10(8) cfu/ml of salmonella typhimurium, escherichia coli o157:h7, or lister ... | 2013 | 23873572 |
| isolation and characterization of listeria monocytogenes isolates from retail foods in shaanxi province, china. | listeria monocytogenes is an important foodborne pathogen of public health concern. a total of 902 retail food samples, including 342 ready-to-eat (rte) foods, 366 infant foods, and 194 raw chickens were collected randomly in supermarkets and farmers' markets in 12 geographic areas in shaanxi province, china and screened for l. monocytogenes. all l. monocytogenes isolates were characterized by antimicrobial susceptibility testing, serotyping, and pulsed-field gel electrophoresis (pfge). twenty-s ... | 2013 | 23841657 |
| efficacy of malic acid against listeria monocytogenes attached to poultry skin during refrigerated storage. | this work evaluated the effect of malic acid washing on the growth of listeria monocytogenes on poultry legs stored at 4°c for 8 d. fresh inoculated chicken legs were dipped into a 1 or 2% malic acid solution (vol/vol) for 5 min or distilled water (control). surface ph values, sensorial characteristics (odor, color, texture, and overall appearance) and l. monocytogenes, mesophile, psychrotroph, and enterobacteriaceae counts were evaluated after treatment (d 0) and after 1, 3, 6, and 8 d of stora ... | 2013 | 23776283 |
| prevalence and quantification of listeria monocytogenes in chicken offal at the retail level in malaysia. | a total of 216 chicken offal samples (chicken liver = 72; chicken heart = 72; chicken gizzard = 72) from wet markets and hypermarkets in selangor, malaysia, were examined for the presence and density of listeria monocytogenes by using a combination of the most probable number and pcr method. the prevalence of l. monocytogenes in 216 chicken offal samples examined was 26.39%, and among the positive samples, the chicken gizzard showed the highest percentage at 33.33% compared with chicken liver (2 ... | 2013 | 23687164 |
| molecular subtyping and virulence gene analysis of listeria monocytogenes isolates from food. | a total of 67 listeria monocytogenes isolates from 698 raw meat samples were characterized for molecular serogroup identification and antimicrobial susceptibility. approximately one third (32.8%) of the isolates belonged to molecular serogroup 1/2a, 3a, followed by 1/2c, 3c (26.9%), 1/2b, 3b, 7 (22.4%), 4b, 4d, 4e (16.4%) and 4a, 4c (1.5%). most of the l. monocytogenes isolates were susceptible to 14 antimicrobials tested but several were resistant to tetracycline, ciprofloxacin and nitrofuranto ... | 2013 | 23628615 |
| ovalbumin-related protein x is a heparin-binding ov-serpin exhibiting antimicrobial activities. | ovalbumin family contains three proteins with high sequence similarity: ovalbumin, ovalbumin-related protein y (ovay), and ovalbumin-related protein x (ovax). ovalbumin is the major egg white protein with still undefined function, whereas the biological activity of ovax and ovay has not yet been explored. similar to ovalbumin and ovay, ovax belongs to the ovalbumin serine protease inhibitor family (ov-serpin). we show that ovax is specifically expressed by the magnum tissue, which is responsible ... | 2013 | 23615912 |
| frequency of salmonella and listeria monocytogenes in five commercial brands of chicken eggs using a combined method of enrichment and nested-pcr. | eggs or egg-based foods, either raw or undercooked, have been identified as vehicles of salmonella outbreaks. the low numbers of salmonella organisms in eggs makes it difficult to detect them in frequency studies. the nested-pcr (n-pcr) technique shows more sensitivity and specificity than bacteriological culture methods (bcms). a preenrichment method followed by enrichment and n-pcr is a good alternative for the investigation of salmonella and listeria monocytogenes in eggs. a total of 2,650 ch ... | 2013 | 23462079 |
| low potential virulence associated with mutations in the inla and prfa genes in listeria monocytogenes isolated from raw retail poultry meat. | packaged raw foods can represent a potential source of listeria monocytogenes contamination when opened at home, and listeriosis is associated with the consumption of undercooked raw foods. the aim of this study was to characterize a group of l. monocytogenes strains isolated from 56 packages of raw chicken meat from a single brand in order to determine the diversity of the strains that dominate in a particular food over time, as well as their pathogenic potential. forty (71%) samples were found ... | 2013 | 23317868 |
| antilisterial properties of marinades during refrigerated storage and microwave oven reheating against post-cooking inoculated chicken breast meat. | this study evaluated growth of listeria monocytogenes inoculated on cooked chicken meat with different marinades and survival of the pathogen as affected by microwave oven reheating. during aerobic storage at 7 °c, on days 0, 1, 2, 4, and 7, samples were reheated by microwave oven (1100 w) for 45 or 90 s and analyzed microbiologically. l. monocytogenes counts on nonmarinated (control) samples increased (p < 0.05) from 2.7 ± 0.1 (day-0) to 6.9 ± 0.1 (day-7) log cfu/g during storage. initial (day- ... | 2013 | 23311403 |
| multiplex fiber optic biosensor for detection of listeria monocytogenes, escherichia coli o157:h7 and salmonella enterica from ready-to-eat meat samples. | listeria monocytogenes, escherichia coli o157:h7 and salmonella enterica are the most common foodborne bacterial pathogens and are responsible for many outbreaks. therefore, multiplex detection of these three using a single assay platform is highly desirable. the objective was to develop and optimize a fiber optic sensor for simultaneous detection of these three from food. the streptavidin coated optical waveguides were immobilized with biotinylated polyclonal antibodies and exposed to the bacte ... | 2013 | 23200648 |
| hospital-acquired listeriosis outbreak caused by contaminated diced celery--texas, 2010. | listeria monocytogenes causes often-fatal infections affecting mainly immunocompromised persons. sources of hospital-acquired listeriosis outbreaks can be difficult to identify. we investigated a listeriosis outbreak spanning 7 months and involving 5 hospitals. | 2013 | 22997210 |