| expression of antisense rna fails to inhibit influenza virus replication. | cell lines were constructed which permanently express influenza virus-specific rna. two approaches were followed. c127 cells were transformed with bovine papilloma virus (bpv) vectors and the resulting cell lines were found to inhibit the replication of influenza virus at low multiplicity of infection (moi 0.05). however, examination of cellular rna using single-stranded probes revealed the presence of both (+)sense and antisense rna transcripts (45-70 copies per cell). in this bpv-based system ... | 1989 | 2481910 |
| analysis of a region of the human cytomegalovirus (ad169) genome coding for a 25-kilodalton virion protein. | in a previous study, we reported the isolation of a lambda gt11 cdna clone (c3) for a virus message that mapped to the hindiii r fragment (human cytomegalovirus [ad169]). in this report, we further analyze transcription from this region of the genome. c3 was used to probe northern (rna) blots of rna isolated from infected cells. two abundant messages, 1.3 and 1.6 kilobases (kb) in size, were detected at 62 h postinfection (p.i.). examination of different time points determined that the 1.6-kb mr ... | 1989 | 2535729 |
| a human cytomegalovirus early gene has three inducible promoters that are regulated differentially at various times after infection. | the long inverted repeat and the adjacent sequences are major early transcription sites of the human cytomegalovirus genome (m. w. wathen and m. f. stinski, j. virol. 41:462-477, 1982). an early transcription unit which flanks the large terminal repeat was analyzed by rna mapping at various times after infection. three unspliced, overlapping rnas were transcribed from different initiation sites and terminated at the same 3' end. individual promoters were isolated for all three transcripts. these ... | 1989 | 2535734 |
| rapid diagnosis of cytomegalovirus by indirect immunofluorescence assay with monoclonal antibody f6b in a commercially available kit. | anti-human cytomegalovirus (cmv) monoclonal antibody (mab) f6b produced by clone 95/12 was used for the rapid diagnosis (16 to 24 h) of cmv isolates in 308 clinical specimens and compared with classic isolation. mab f6b gave 100% correlation with isolation. when this mab was used for direct diagnosis with 212 urine and bronchoalveolar specimens, detection of cmv varied from 50 to 75% compared with viral isolation. | 1989 | 2536759 |
| electric field-induced cell fusion and human monoclonal antibodies. | a persistent problem in the generation of antigen-specific human monoclonal antibodies is the rarity of appropriate b cells in human blood or splenic tissues. in order to immortalize the rare antigen-specific cells that are available, an electric field-induced cell fusion technique has been shown to markedly increase the fusion efficiency in comparison to polyethylene glycol-induced cell fusion using epstein-barr virus (ebv) or pokeweed mitogen activated b cells. fusion efficiency of 10(-3)-10(- ... | 1989 | 2536773 |
| regulation of cytomegalovirus late-gene expression: differential use of three start sites in the transcriptional activation of icp36 gene expression. | we have investigated the transcriptional regulation of the human cytomegalovirus gamma gene encoding the icp36 family (p52, the major late dna-binding protein). the icp36 transcription unit initiates at three distinct sites which are separated by approximately 50 nucleotides and are differentially regulated during infection. at early times (8 h postinfection), only two of these start sites, the most proximal and distal site, were active whereas at late times (36 h postinfection), the middle star ... | 1989 | 2538657 |
| multiplicity dependent expression of the predominant phosphoprotein pp65 of human cytomegalovirus. | recent clinical isolates of human cytomegalovirus (hcmv) display considerable quantitative differences in the expression of the structural phosphoprotein pp65. this study shows that a reduced production of pp65 correlates with reduced amounts of its coding transcript in wild-type strains compared to the high level expression in the laboratory strain ad169. the cleavage pattern of the encoding dnas including the promoter region did not show major differences among the isolates. therefore the vari ... | 1989 | 2539706 |
| protective effects of towne cytomegalovirus vaccine against low-passage cytomegalovirus administered as a challenge. | to test the protective effect of towne live attenuated human cytomegalovirus (hcmv) vaccine in normal individuals, we developed a parenteral challenge consisting of a low-passage isolate (toledo stain) inoculated subcutaneously in graded doses. this challenge virus caused a mild mononucleosis syndrome in seronegative individuals at doses of 10 or 100 pfu. the illness was accompanied by atypical lymphocytosis, raised hepatic enzymes, excretion of hcmv and hcmv-specific immune responses. naturally ... | 1989 | 2540247 |
| genomic locus for a 140 kda structural protein (pp150) of human cytomegalovirus in strains towne and ad169. | an hcmv specific clone was isolated from a genomic library of human cytomegalovirus (hcmv) dna cloned into the expression vector lambda gt11. this clone (lambda 111-1) expressed an hcmv/beta-galactosidase fusion protein which was reactive with rabbit antibody prepared against purified hcmv virions and dense bodies as well as human hcmv immune serum. by probing western blots of hcmv virion proteins or hcmv-infected cells with antibody prepared against the fusion protein, the authentic gene produc ... | 1989 | 2541578 |
| detection of cytomegalovirus dna in short term cultures. | detection of human cytomegalovirus (cmv) by in situ dna hybridisation six days after incubation of human diploid fibroblasts (isdh-6) was evaluated prospectively in 205 urine samples, obtained from 57 kidney transplant and 17 bone marrow transplant recipients. the results were compared to those of conventional virus isolation (cvi) and the detection of cmv early antigens after one day of cultivation (ea-1). of 42 samples positive for cmv by at least one of these methods, 40 (95%) were detected w ... | 1989 | 2542353 |
| the human cytomegalovirus mtrii colinear region in strain tanaka is transformation defective. | the morphological transforming region ii (mtrii) of human cytomegalovirus (hcmv) strain towne has been localized to a 980-base-pair fragment containing three putative open reading frames (orfs) of 79, 83, and 34 amino acids (aa). in addition, noncoding dna sequence elements which have the potential to form stem-loop structures were also observed within mtrii. to determine what elements within hcmv towne mtrii are important in transformation, colinear regions in other hcmv strains (ad169 and tana ... | 1989 | 2542596 |
| posttranscriptional regulation of a class of human cytomegalovirus phosphoproteins encoded by an early transcription unit. | we have further characterized and determined the origins of a family of nuclear phosphoproteins of 84, 50, 43, and 34 kilodaltons (kda) encoded by a class of early transcripts arising from the adjacent ecori fragments r and d (map units 0.682 to 0.713) of the strain ad169 human cytomegalovirus genome. these rnas have a complex spliced structure with common 5' and internal exons and alternative 3' exons with coterminal 3' ends. at early times, two fully processed species of 2.1 and 2.2 kilobases ... | 1989 | 2542619 |
| restriction endonuclease analysis of human cytomegalovirus isolated in immunocompromised patients. | cytomegalovirus is frequently isolated from immunosuppressed patients or from patients with acquired immunodeficiency syndrome. twelve patients were studied for several months. in 7 patients more than 1 cytomegalovirus was isolated; in one of these patients three viruses were isolated, two of them show small differences in their migration pattern and the third seems to be a different strain after restriction analysis of cmv genome. this could reflect variation in the genome of the cmv virus as i ... | 1989 | 2542869 |
| the folate antagonist, methotrexate, is a potent inhibitor of murine and human cytomegalovirus in vitro. | cytomegalovirus (cmv) is a major source of morbidity for immunocompromised patients, such as aids patients. the folic acid antagonists have not been explored as potential antiviral agents against cmv. we examined the effects of methotrexate, compared to acyclovir and ganciclovir, on both murine cmv (mcmv) and human cmv (hcmv) in vitro. using a plaque assay in mouse embryo cells or human foreskin fibroblasts for mcmv and hcmv respectively, we found that methotrexate, in micromolar concentrations, ... | 1989 | 2543289 |
| expression of oncogenic ras in human teratocarcinoma cells induces partial differentiation and permissiveness for human cytomegalovirus infection. | retinoic acid (ra)-induced differentiation of human teratocarcinoma (t2) cells results in a change from a normally non-permissive phenotype for human cytomegalovirus (hcmv) infection to cells which are fully permissive. we have used this system to analyse factors associated with differentiation which may regulate hcmv gene expression. differentiation of t2 cells results in an increase of c-ras expression. consequently, we have introduced ras expression vectors into t2 cells. we find that, as wit ... | 1989 | 2543742 |
| human cytomegalovirus and monocytes: limited infection and negligible immunosuppression in normal mononuclear cells infected in vitro with mycoplasma-free virus strains. | human cytomegalovirus (hcmv) infection has previously been associated with the production of immunosuppression. the mechanism by which any such immunosuppressive effect might be mediated is unclear but previous work has implicated an effect of the virus on monocytes. we have attempted to characterize the immunosuppressive activity produced by in vitro infection of normal monocytes with hcmv strain ad169. we first examined the ability of hcmv ad169 and recent clinical isolates to infect normal pe ... | 1989 | 2543753 |
| identification and preliminary use of recombinant lambda gt11 fusion proteins in human cytomegalovirus diagnosis. | we have isolated reactive clones from a lambda gt11 expression library of human cytomegalovirus (hcmv) dna using hcmv-positive human sera. among the recombinant clones obtained, one carried a fragment encoding a portion of p52, the major non-structural dna-binding protein of 52k (p52) and another carried a part of the gene coding for pl50, the major structural phosphoprotein. these two fusion proteins were examined by immunoblot analysis to test their ability to bind specific antibodies in human ... | 1989 | 2543778 |
| synthesis and antiviral activity of the nucleotide analogue (s)-1-[3-hydroxy-2-(phosphonylmethoxy)propyl]cytosine. | the acyclic nucleotide analogue (s)-1-[3-hydroxy-2-(phosphonylmethoxy)propyl] cytosine (2, hpmpc) was prepared on a multigram scale in 18% overall yield starting from (r)-2,3-o-isopropylideneglycerol. the key step in the nine-step synthetic route is coupling of cytosine with the side-chain derivative 8 which bears a protected phosphonylmethyl ether group. in vitro data showed that hpmpc has good activity against herpes simplex virus types 1 and 2, although it was 10-fold less potent than acyclov ... | 1989 | 2544723 |
| a subset of herpes simplex virus replication genes induces dna amplification within the host cell genome. | herpes simplex virus (hsv) induces dna amplification of target genes within the host cell chromosome. to characterize the hsv genes that mediate the amplification effect, combinations of cloned dna fragments covering the entire hsv genome were transiently transfected into simian virus 40 (sv40)-transformed hamster cells. this led to amplification of the integrated sv40 dna sequences to a degree comparable to that observed after transfection of intact virion dna. transfection of combinations of s ... | 1989 | 2547992 |
| enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction. | polymerase chain reaction (pcr) amplification was used to detect human cytomegalovirus (hcmv) sequences. the fragments selected for amplification were fragments of 130 and 152 base pairs (bp) located at two opposite ends of hcmv strain ad169 ecori fragment d. amplification of the 152-bp dna was consistently greater than that of the 130-bp dna. at the optimal mg2+ concentration of 5 mm, specific pcr amplification of 152-bp dna with taq polymerase was sensitive; only one ad169-infected fibroblast ... | 1989 | 2549097 |
| protein analysis of herpes simplex virus latency in vitro established with cycloheximide. | herpes simplex virus (hsv)-specific protein synthesis was examined during establishment of hsv latency and reactivation of virus in human embryonic lung cells treated with cycloheximide and incubated at 40.5 degrees. eight viral proteins, identified during the first two days of establishment of latency at 40.5 degrees, were undetectable by day 3. at least two synthesized proteins were present during the maintenance phase of latency. reactivation of hsv (viral protein 135k) was first detected in ... | 1989 | 2549718 |
| myocardial involvement due to a disseminated human cytomegalovirus infection in a heart transplant recipient. a case report. | a cytomegalovirus seronegative 9 year-old child, affected by terminal dilated cardiomyopathy, underwent cardiac transplantation and received the heart of a cytomegalovirus seropositive donor. after the positive outcome of an acute moderate rejection episode which was treated with steroids, the patient developed an infectious clinical syndrome with cytomegalovirus seroconversion and virus isolation from peripheral blood leukocytes. the 5th endomyocardial biopsy showed a typical cytomegalovirus-li ... | 1989 | 2550305 |
| properties of igg subclasses to human cytomegalovirus. | the igg subclass of antiviral antibodies to human cytomegalovirus (cmv) is mainly of igg1 type. most cmv seropositive sera also have virus-specific igg3, but of a lower titre as measured by enzyme-linked immunosorbent assay (elisa). we studied the reactivity pattern of these two igg subclasses to cmv structural polypeptides in order to define how virus-specific igg1 and igg3 contribute to the neutralization of cmv. neutralization of cmv was performed with cmv igg1 and igg3 separated from cmv ser ... | 1989 | 2550502 |
| human cytomegalovirus rnas immunoprecipitated by multiple systemic lupus erythematosus antisera. | the association of human cytomegalovirus (hcmv) rnas with ribonucleoprotein particles that react with antibodies from patients with systemic lupus erythematosus was tested by immunoprecipitation with multiple patients' sera. a major late 2.8 kb rna and several minor rnas encoded by the hcmv long repeat region were immunoprecipitated from hcmv-infected cells by la, ro and, much less abundantly, sm autoimmune antisera. the exact location of these rnas was determined by high resolution r-loop mappi ... | 1989 | 2550574 |
| induction by sodium butyrate of cytomegalovirus replication in human endothelial cells. | cultured endothelial cells are shown to be induced in regard to permissiveness to human cytomegalovirus by temporary treatment postinfection with sodium butyrate (1-2 mm). drug-treated cells are demonstrated to exhibit expression of immediate early and early viral antigens, synthesis of viral dna and viral structural glycoprotein b. progeny virus could be visualized by electron microscopy. | 1989 | 2552958 |
| immunohistochemical detection of an immediate early antigen of human cytomegalovirus in normal tissues. | as a member of the herpesvirus family, human cytomegalovirus (hcmv) induces a life-long latent infection in most individuals infected with it. this latent infection is subject to periodic reactivations that serve as an important source of disease and death in patients with defective immunologic responses. the specific types of cells that harbor latent hcmv have not yet been identified. to detect latent hcmv and identify the host cells carrying it, tissue sections from nine hcmv-seropositive norm ... | 1989 | 2553823 |
| molecular cloning of dna sequences from cervical intraepithelial neoplasia that hybridize to human cytomegalovirus dna. | we have prepared embl3 libraries of dna extracted from the cervix of a patient with cervical intraepithelial neoplasia (cin) and isolated seven recombinant clones containing sequences that hybridize to human cytomegalovirus (hcmv) dna. restriction analysis of one clone with bamhi and sali endonucleases revealed that the insert dna showed a high degree of homology to the hcmv ad169 genome over the region between the hindiii k/e site and the sali site located within the bamhi p fragment. the hcmv ... | 1989 | 2554581 |
| seroepidemiologic studies of cytomegalovirus and epstein-barr virus infections in relation to human immunodeficiency virus type 1 infection in selected recipient populations. transfusion safety study group. | antibodies to human cytomegalovirus (cmv) and epstein-barr virus (ebv) were evaluated among 1,171 persons with and without antibodies to human immunodeficiency virus type 1 (anti-hiv-1). these included 97 blood donors, 577 persons given blood components or products, and 497 controls. a significantly higher proportion of anti-hiv-1 positive than -negative donors were anti-cmv-positive, a finding associated with homosexual contact among some of the former. among subjects with treated clotting diso ... | 1989 | 2555471 |
| disparate effects of two herpesvirus [corrected] immediate-early gene trans-activators on the hiv-1 ltr. | the bmlf1 region of the epstein-barr virus (ebv) genome and the immediate-early (ie) region of human cytomegalovirus (hcmv) both encode proteins which can trans-activate heterologous promoter/chloramphenicol acetyl transferase (cat) constructs, including a human immunodeficiency virus type-1 promoter/cat construct. we demonstrate here that this trans-activation by the ebv bmlf1 gene product, which we have previously shown to be largely post-transcriptional, is reporter gene dependent. in contras ... | 1989 | 2556854 |
| complement-independent neutralising monoclonal antibody with differential reactivity for strains of human cytomegalovirus. | a mouse monoclonal antibody with complement-independent neutralising activity against cytomegalovirus (cmv) and reactive with the 86 kilodalton (kda) viral glycoprotein h is described. neutralisation tests against a range of different strains of cmv showed significant crossreactivity, but clear differences were evident between the two prototype viruses ad169 and davis, and particularly between ad169 and several low-passage recent clinical isolates; cmv present in urine was neutralised weakly if ... | 1989 | 2557384 |
| detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients. | peripheral blood polymorphonuclear (pmn) cells from 35 immunocompromised patients (22 heart transplant recipients and 13 aids patients) and four normal subjects were tested for the presence of human cytomegalovirus (hcmv) immediate early antigen (iea) (antigenemia) by indirect immunofluorescence (ifa) and iea-specific monoclonal antibodies (mab). pmn samples were tested in parallel for hcmv isolation (viremia) by using mab to viral early antigens (ea) and the ifa technique 24-48 hr after inocula ... | 1989 | 2557386 |
| characteristics of mnng induced repair synthesis and dna synthesis induced by human cytomegalovirus. | dna synthesis induced by n-methyl-n'-nitro-n-nitrosoguanidine in mouse embryo and human embryo cells was compared with dna synthesis induced in these cells by human cytomegalovirus. in virus infected human embryo cells grown in the medium depleted of arginine dna synthesis showed resistance to hydroxyurea and arabinofuranosylcytosine, similarly as repair synthesis induced by mnng. dna synthesis induced by the virus in mouse embryo cells was partially sensitive to both inhibitors. | 1989 | 2559565 |
| anti-herpesvirus activity of carbocyclic oxetanocin g in vitro. | a series of new compounds, carbocyclic oxetanocins, have been synthesized and their anti-herpesvirus activity determined. carbocyclic oxetanocin g (oxt-g) was most active against herpes simplex virus (hsv) and human cytomegalovirus (hcmv) among carbocyclic oxetanocins tested; the median effective concentrations (ec50) for hsv-1, -2, and hcmv were 0.23, 0.04 and 0.40 micrograms/ml, respectively. the ec50 value of carbocyclic oxt-g against hsv-2 was significantly lower than those of acyclovir, gan ... | 1989 | 2559911 |
| high levels of expression of full length human pro-alpha 2(v) collagen cdna in pro-alpha 2(v)-deficient hamster cells. | a full length cdna encoding human pro-alpha 2(v) collagen was constructed. partial sequencing of the cdna and primer extension analysis of mrna from fibroblasts found that pro-alpha 2(v) mrna differs from the mrnas of other fibrillar collagens in the increased length of its 5'-untranslated region. the pro-alpha 2(v) cdna was placed downstream of the human cytomegalovirus immediate early promoter/regulatory sequences for expression studies in cultured chinese hamster lung cells. these cells have ... | 1989 | 2584236 |
| improved retroviral vectors for gene transfer and expression. | we describe a set of murine retrovirus-based vectors that include unique cloning sites for insertion of cdnas such that the cdna can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter. the vectors carry the neomycin phosphotransferase gene expressed from an alternate promoter as a selectable marker. these vectors have been constructed to prevent viral protein synthesis from the remaining viral sequ ... | 1989 | 2631796 |
| treatment of cytomegalovirus retinopathy with ganciclovir. | ganciclovir is an experimental antiviral drug with activity against human cytomegalovirus (cmv). forty patients with acquired immune deficiency syndrome (aids) and cmv retinopathy were treated with ganciclovir on a compassionate protocol basis. initial treatment doses ranged from 5.0 to 14.0 mg/kg/day for 9 to 26 days. signs of drug response were a halt to enlargement of lesions, decreased opacification of retinal tissue, and resolution of hemorrhage and vasculitis. complete response was seen in ... | 1987 | 2821464 |
| human cytomegalovirus structural components: intracellular and intraviral localization of p28 and p65-69 by immunoelectron microscopy. | using specific monoclonal antibodies, we have localized two structural proteins (p65-69 and p28) of human cytomegalovirus in infected cells and in virions and/or virus-related particles by immunoelectron microscopy using protein a-gold. protein p65-69 is present in some roundish structures in the nuclei, often in contact with the viroplasm, and in the cytoplasm, exclusively within the dense body matrix. protein p28 is present only in the outline of cytoplasmic capsids, and reaches the highest de ... | 1987 | 2821705 |
| resistance to methylation de novo of the human cytomegalovirus immediate early enhancer in a model for virus latency and reactivation in vitro. | rat-9g cells carry several stably integrated copies of the major immediate early (ie) transcription unit of the human cytomegalovirus (hcmv). in these cells ie expression is repressed but inducible. in this report we describe the dna methylation status of hpaii, hhai and ahaii sites within the ie gene, determined at different passage levels. most, if not all, of the resident ie genes were progressively methylated in a similar fashion. this resulted in dna methylation patterns in which sites surr ... | 1987 | 2824664 |
| human cytomegalovirus (hcmv) enhances bovine papilloma virus (bpv) transformation in vitro. | infection of nih 3t3 cells with a combination of hcmv and bpv resulted in more foci than infection with bpv alone. foci were microscopically apparent at 4 days in the mixed infection and did not appear until 2 days later in the cultures infected with bpv alone. the enhancement was abolished by heat inactivation of the hcmv and also when the hcmv was replaced by a "mock inoculum." southern blot analysis of cellular dna from transformed cells showed a similar amount of extrachromosomal bpv dna in ... | 1987 | 2824682 |
| four phosphoproteins with common amino termini are encoded by human cytomegalovirus ad169. | in this report, we identify the proteins encoded by the 2.2-kilobase class of early transcripts arising from a region of the strain ad169 human cytomegalovirus genome (map units 0.682 to 0.713) which contains cell-related sequences. these transcripts, encoded by adjacent ecori fragments r and d, have a complex spliced structure with 5' and 3' coterminal ends. antiserum directed against a synthetic 11-amino-acid peptide corresponding to the predicted amino terminus of the proteins was generated a ... | 1988 | 2824853 |
| ultrastructural characterization of a new human b lymphotropic dna virus (human herpesvirus 6) isolated from patients with lymphoproliferative disease. | a new dna virus, designated "human b lymphotropic virus" or "human herpesvirus 6" (hblv), has been isolated from the peripheral blood leukocytes of patients with various lymphoproliferative disorders, in some instances also associated with human immunodeficiency virus (hiv-1) infection. hblv, propagated in vitro in human cord blood lymphocytes, was found to be ultrastructurally similar to members of the herpesvirus family. it is an enveloped virus with an icosahedral nucleocapsid made up of 162 ... | 1987 | 2824914 |
| inhibition of antibody-dependent cell-mediated cytolysis of tumor cells by fc receptor of human cytomegalovirus. brief report. | the presence of hcmv-infected cells inhibits the killing of mouse tumor cells by adherent pec in an antibody-dependent cell-mediated cytotoxicity (adcc) test system. the effect of hcmv-infected cells on adcc is mediated by the binding of igg to the fc receptors of infected cells. | 1987 | 2825621 |
| human immune responses to major human cytomegalovirus glycoprotein complexes. | sera from both human cytomegalovirus (hcmv)-seropositive adults and infants with congenital hcmv infection recognized two major hcmv glycoprotein complexes. however, proliferative responses of peripheral blood mononuclear cells to these complexes varied among seropositive adults and were not detected in any of the infants. thus, these glycoproteins alone may not be sufficient to develop a subviral hcmv vaccine. | 1988 | 2828655 |
| negative and positive regulation by a short segment in the 5'-flanking region of the human cytomegalovirus major immediate-early gene. | to analyze the significance of inducible dnase i-hypersensitive sites occurring in the 5'-flanking sequence of the major immediate-early gene of human cytomegalovirus (hcmv), various deleted portions of the hcmv immediate-early promoter regulatory region were attached to the chloramphenicol acetyltransferase (cat) gene and assayed for activity in transiently transfected undifferentiated and differentiated human teratocarcinoma cells, tera-2. assays of progressive deletions in the promoter regula ... | 1987 | 2828927 |
| an ebv-based mammalian cell expression vector for efficient expression of cloned coding sequences. | the construction of a mammalian cell expression vector using human cytomegalovirus immediate early gene enhancer to initiate transcription of inserted coding sequences is described. the vector also carries epstein-barr virus ebna-1 nuclear antigen gene, ori-p sequences and hygromycin b resistance gene hph from e. coli. the expression capacity of this construct was tested by inserting the chloramphenicol acetyltransferase (cat) gene into the vector. the ebv-cat construct was transfected into vari ... | 1988 | 2829966 |
| application of a new medium supplement for propagation and storage of human cytomegalovirus. | a new medium supplement, nu-serum, was evaluated for cultivation of human embryonic lung fibroblasts (hel) and for propagation and storage of human cytomegalovirus (hcmv). nu-serum was comparable to fetal bovine serum (fbs) in promoting rapid growth of hel if they were seeded at a sufficient density. hcmv replicated quite satisfactorily in hel cultured with media supplemented with nu-serum as well as fbs. inactivation of hcmv at 37 c occurred similarly when the medium contained fbs or nu-serum. ... | 1987 | 2830472 |
| cytomegalovirus replicates efficiently in human kidney mesangial cells. | human cytomegalovirus (hcmv) is a major renal pathogen in congenitally infected infants and renal allograft recipients. we postulated that a specific renal cell type was involved in hcmv infection and reactivation. human fetal kidney cortex cell cultures were assayed for their ability to support hcmv infection. infectious center assays indicated that the low level of viral replication observed by virus yield assay occurred from a fraction of the cells in the mixed cultures. virus-specific immuno ... | 1988 | 2830624 |
| a gene for dihydrofolate reductase in a herpesvirus. | the enzyme dihydrofolate reductase (dhfr) is found ubiquitously in both prokaryotes and eukaryotes. it is essential for de novo synthesis of purines and of deoxythymidine monophosphate for dna synthesis. among viruses, however, only the t-even and t5 bacteriophage have been found to encode their own dhfr. in this study a gene for dhfr was found in a specific subgroup of the gamma or lymphotropic class of herpesviruses. dna sequences for dhfr were found in herpesvirus saimiri and herpesvirus atel ... | 1988 | 2830673 |
| effects of herpesvirus infections on the chemiluminescence induced by zymosan phagocytosis in mouse peritoneal macrophages. | the chemiluminescence (cl) induced by zymosan phagocytosis was tested in mouse peritoneal macrophages infected with three different types of herpes viruses: herpes simplex type-1 (hsv-1), human cytomegalovirus (hcmv) and murine cytomegalovirus (mcmv). the intensity of cl was tested in various intervals of virus infections. in the first eight hours zymosan induced chemiluminescence decreased in all the three systems. by the 24th hour, the macrophages infected with hcmv had almost completely recov ... | 1987 | 2830762 |
| human cytomegalovirus-specific cytotoxic t cells: their precursor frequency and stage specificity. | human virus-specific cytotoxic t (tc) cells may be important in maintaining the virus/host equilibrium during persistent herpes virus infections such as that with human cytomegalovirus (hcmv). we have previously shown that hcmv-specific tc cells are present in peripheral blood in normal asymptomatic seropositive individuals (l. k. borysiewicz et al., eur. j. immunol. 1983. 13: 804). in this study we have used limiting dilution analysis to estimate the precursor frequency of these tc cells and to ... | 1988 | 2832181 |
| a 46 kd polypeptide, present in purified human cytomegalovirus, is provided with dnase activity and is antigenically related to a higher molecular weight, enzymatically inactive, cellular protein. | dnase activity can be detected in lysates of extensively purified human cytomegalovirus (hcmv) particles. the presence of activity only in purified virions and not in dense bodies and its neutralization by hcmv-positive human sera strongly suggest that the enzyme is virus-specific. in situ analysis of the enzymatic activity of individual hcmv polypeptides, separated in a dna-containing polyacrylamide gel, has shown that the dnase activity present in purified virions is mainly associated with a p ... | 1988 | 2832711 |
| characterization of two different human cytomegalovirus glycoproteins which are targets for virus neutralizing antibody. | in previous studies we have identified two viral polypeptides detected by murine monoclonal antibodies which neutralize the infectivity of human cytomegalovirus (cmv) ad169. one is an 86,000-da polypeptide (p86) and the second is a complex of two major coimmunoprecipitating polypeptides of 130,000 and 55,000 da (p130/55). in this study we have shown that the two viral polypeptides are immunologically unrelated and have distinct peptide cleavage patterns. we have characterized these polypeptides ... | 1988 | 2833009 |
| comparison of cytomegalovirus antibodies by means of cf, elisa and immunoblotting techniques. | antibody to human cytomegalovirus in sera of immune blood donors reacted with 12 viral polypeptides of molecular weight 200.000. 150.000, 65.000, 48.000, 34,000. 25.000, 23.000, 21.000 and 18.000. development of cmv infection in renal transplant recipients resulted in increase of antibody reacting with various cmv polypeptides as well as in intensity of this reaction. the highly comparable results for cmv antibody detecting were obtained with immunoblotting and elisa techniques. | 1987 | 2833198 |
| the use of elisa and nonradioactive dna hybridization assays for the detection of human cytomegalovirus. | a hybridization assay using a biotinylated dna probe was compared to both elisa and direct isolation methods for detecting human cytomegalovirus (hcmv). the biotin labeled hcmv ad 169 hindiii-o-dna fragment was used in a dot-blot assay to screen for the presence of hcmv in 186 urine specimens obtained from kidney transplant patients. the biotinylated hcmv hindiii-o probe could detect 3 log10 tcid50 units of hcmv. urine specimens were also examined for the presence of hcmv by either elisa or dire ... | 1988 | 2836464 |
| induction of gene expression under human cytomegalovirus immediate early enhancer-promoter control by inhibition of protein synthesis is cell cycle-dependent. | in this paper we describe stably transfected rat cell lines which harbour either the human cytomegalovirus (hcmv) immediate early (ie) gene encoding the 72k ie nuclear antigen (iea) or the bacterial chloramphenicol acetyltransferase (cat) gene both under transcriptional control of the hcmv ie enhancer-promoter (-484 to -19 relative to the ie cap site, +1). in these cell lines ie gene or cat gene expression is repressed but can be induced by heat-shock, by sodium arsenite and by inhibitors of pro ... | 1988 | 2838570 |
| association of cytomegalovirus infection with autoimmune type 1 diabetes. | the lymphocytes from 59 newly diagnosed type 1 diabetic patients and 38 normal control subjects were examined for the presence of human cytomegalovirus (cmv) genome by molecular hybridizations with human cmv specific probe. the cmv specific viral genome was found in 13 (22%) of 59 diabetic patients, but in 1 (2.6%) of 38 control subjects. of the patients, 39% had islet cell antibody (ica) and 41% had cytotoxic beta cell surface antibody (cbsa) in their serum; of the controls the corresponding ra ... | 1988 | 2898620 |
| on the intracellular transport and the nuclear association of human cytomegalovirus structural proteins. | in cells productively infected with human cytomegalovirus (hcmv) ad169, large amounts of two viral proteins, the 150k major capsid and the 68k major matrix proteins, are continuously produced during the late phase of infection. in the present study, the mechanism for the intracellular transport of the 150k and 68k proteins was investigated. infected cells were labelled for 30 min at 72 h post-infection with [35s]methionine, chased for various periods of time at 37 degrees c, and fractionated int ... | 1985 | 2984313 |
| the structure of the major immediate early gene of human cytomegalovirus strain ad169. | the nucleotide sequence of the major immediate early (ie) gene of human cytomegalovirus strain ad169 was determined. the structure of the gene was examined by nuclease mapping and by sequence analysis of a cdna clone made from ie mrna. the gene encodes a spliced molecule of 1736 nucleotides, made up of four exon sequences of 121, 88, 185 and 1342 nucleotides. three introns (827, 114 and 170n) were located near the 5' end of the gene. a single open reading frame starting in the second exon extend ... | 1985 | 2986374 |
| catalytic properties of a human cytomegalovirus-induced protein kinase. | human cytomegalovirus, a dna virus whose genome contains a fragment of transforming dna, induces a threonine-serine protein kinase having a molecular mass of 68 kda (p68). p68 was extracted from cells 96-144 h after infection, and immunoprecipitated with a monoclonal antibody (f6b). antibody-enzyme complexes were immobilized on heat/formaldehyde-inactivated staphylococcus aureus. the best substrates for p68 were acidic proteins, phosvitin and casein. glycogen synthase, phosphorylase alpha and hi ... | 1985 | 2986975 |
| cell-related sequences in the dna genome of human cytomegalovirus strain ad169. | human cytomegalovirus (hcmv) cloned ecori fragments r and b hybridized strongly, under standard high-stringency conditions, to uninfected cellular dna of human, murine, or sea urchin origin. less hybridization was detected with fragments, a, c, e, wl(f), wn(h), i, m, o, p, q, v, c, d, and e. southern blot analysis of the hcmv-related human dna localized the major sites of hybridization of hcmv ecori fragments r, b, and d to defined regions of the 28s rrna gene. | 1985 | 2991601 |
| a very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. | a strong transcription enhancer was identified in the genomic dna (235 kb) of human cytomegalovirus (hcmv), a ubiquitous and severe pathogen of the herpesvirus group. cotransfection of enhancerless sv40 dna with randomly fragmented hcmv dna yielded two sv40-hcmv recombinant viruses that had incorporated overlapping segments of hcmv dna to substitute for the missing sv40 enhancer. within hcmv, these enhancer sequences are located upstream of the transcription initiation site of the major immediat ... | 1985 | 2985280 |
| precise localization of genes on large animal virus genomes: use of lambda gt11 and monoclonal antibodies to map the gene for a cytomegalovirus protein family. | we describe an efficient procedure, which uses monoclonal antibodies directed against specific viral proteins, for the precise mapping of genes on large dna virus genomes. we have used the technique to locate the gene encoding a family of antigenically related dna-binding proteins on the 240-kilobase-pair human cytomegalovirus (cmv) genome. a random library of cmv dna fragments was generated using the prokaryotic vector lambda gt11, which expresses open reading frames as beta-galactosidase fusio ... | 1985 | 2983334 |
| inhibition of cellular dna polymerase alpha and human cytomegalovirus-induced dna polymerase by the triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine. | the triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine were examined for their inhibitory effect on highly purified cellular dna polymerase alpha and human cytomegalovirus (towne strain)-induced dna polymerase. these two nucleoside triphosphates competitively inhibited the incorporation of dgmp into dna catalyzed by the dna polymerases. the virus-induced dna polymerase had greater binding affinity for the triphosphate of 9-(2-hydroxyethoxymethyl)guanin ... | 1985 | 2983088 |
| early and late antigens of human cytomegalovirus: electroimmunodiffusion assay of numbers, relationships, and reactivities with donor sera. | early antigens (ea) of human cytomegalovirus extracted from cytosine arabinoside-blocked cells infected with 0.01-20 infectious units (iu)/cell were assayed with human serum by electroimmunodiffusion (eid). the number of detectable ea types increased from one to eight as the iu/cell was raised from 0.01 to 10. there was no increase in the number of ea with further increases in iu/cell, with prolonged culture, or when detergent was included in the extraction buffer. at least five of the eight ea ... | 1985 | 2983008 |
| identification and nucleotide sequence of the glycoprotein gb gene of equine herpesvirus 4. | the nucleotide sequence of the glycoprotein gb gene of equine herpesvirus 4 (ehv-4) was determined. the gene was located within a bamhi genomic library by a combination of southern and dot-blot hybridization with probes derived from the herpes simplex virus type 1 (hsv-1) gb dna sequence. the predominant portion of the coding sequences was mapped to a 2.95-kilobase bamhi-ecori subfragment at the left-hand end of bamhi-c. potential tata box, cat box, and mrna start site sequences and the translat ... | 1989 | 2915378 |
| detection of human cytomegalovirus dna and viral antigens in tissues of different manifestations of cmv infection. | biopsy and autopsy specimens from 22 patients with cytomegalovirus (cmv) infections were investigated by means of in situ hybridization (ish) to detect viral dna and by immunohistochemistry (ihc) to visualize viral proteins. both methods proved to be valuable tools for histopathology. ish sometimes recognized cells that did not show typical cmv inclusions. an antiserum against the full spectrum of viral proteins (non-infectious enveloped particles) detected most cytomegalic cells in disseminated ... | 1988 | 2901164 |
| human antibody response to human cytomegalovirus-specific dna-binding proteins. | human antibody responses to human cytomegalovirus (hcmv) specific dna-binding proteins were studied in serum samples by the western blot technique. the molecular weights of six dna-binding proteins found in hcmv-infected cells, ranged from 52kd to 18kd. the sera obtained from patients with acute hcmv infections reacted well with the six hcmv specific dna-binding proteins. the strongest reactivity was observed with the 52kd and 35kd proteins. the sera from healthy hcmv seropositive donors reacted ... | 1988 | 2897767 |
| antibodies to human cytomegalovirus structural polypeptides during primary infection. | this study aimed at broadening the understanding of the immunogenic potential of cytomegalovirus (cmv) structural polypeptides during natural infection and to ascertain their possible use in serological diagnosis. immunoblotting was used to analyse the appearance and development of serum igg and igm against human cmv structural polypeptides in sequential sera from renal transplant recipients during the first 1-3 months of primary cmv infection. the results showed that the first igg to appear is ... | 1988 | 2851606 |
| detection of cytomegalovirus in bronchial lavage and urine using a monoclonal antibody to an hcmv early nuclear protein. | laboratory diagnosis of 24 cases of human cytomegalovirus (hcmv) infection in patients with the acquired immunodeficiency syndrome, renal transplant recipients and premature infants was achieved. these results were obtained by a rapid, sensitive and versatile hcmv-antigen detection method, which combined cell culture and immunoperoxidase staining with a monoclonal antibody to an hcmv "early" nuclear protein. the results were compared with hcmv isolation by the conventional cell culture method. w ... | 1988 | 2850996 |
| interaction of herpes simplex virus type 2 with a rat glioma cell line. | the interaction between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and two neural cell lines, mouse neuroblastoma (n1e-115) and rat glioma (c6-bu-1), was investigated. n1e-115 cells were permissive to both types of hsv. in c6-bu-1 cells, on the other hand, all the hsv-1 strains tested so far showed persistent infection, and the infectious virus of hsv-2 strains disappeared spontaneously. the hsv-2-infected c6-bu-1 cells were positive for hsv-2-specific dna sequences, virus-specific r ... | 1988 | 2850449 |
| detection of human cytomegalovirus by slot-blot hybridisation assay employing oligo-primed 32p-labelled probe. | a 32p-labelled hind iii-0 dna fragment (nine kilobases; kb) from human cytomegalovirus ad-169 (hcmv) was used in slot-blot hybridisation assay for the detection of hcmv in clinical samples. the results obtained with dna hybridisation assay (dna ha) were compared with virus isolation using conventional tube cell culture (ctc) and centrifugation vial culture (cvc), immunofluorescence (if), and complement fixation test (cft). of 15 ctc-positive samples, 13 were positive with dna ha (sensitivity 86. ... | 1988 | 2850344 |
| effect of natural human interferon-beta on the replication of human cytomegalovirus. | the antiviral effect of natural human interferon-beta (huifn-beta) against human cytomegalovirus (cmv) was evaluated in human embryonic lung fibroblasts (hel). natural huifn-beta, like other huifns, inhibited the replication of cmv. pretreatment of the cells with natural huifn-beta inhibited the appearance of immediate-early antigen (iea) or pre-early nuclear antigen (pena) as well as the production of infectious cmv. after a single treatment with natural huifn-beta, intracellular 2', 5'-oligoad ... | 1988 | 2850341 |
| interactions of human cytomegalovirus with leukocytes in vivo: analysis by in situ hybridization. | reactivation of human cytomegalovirus (hcmv) from latency occurs in immunosuppressed individuals and infection is itself immunosuppressive. to better understand the basis for this virally induced impairment of immune function, we have analyzed virus-leukocyte interactions by in situ hybridization. we detected viral dna in 12 viremic patients in the mononuclear cell population, predominantly in cells identified as monocytes by their morphology and by labelling the cells with a monocyte specific m ... | 1987 | 2849021 |
| prophylaxis of serious cytomegalovirus infection in renal transplant candidates using live human cytomegalovirus vaccine. interim results of a randomized controlled trial. | we report the interim results of a randomized, double-blind, placebo-controlled, clinical trial of prophylactic, live, attenuated cytomegalovirus (cmv) vaccination (towne strain of cmv) of renal transplant candidates (rtcs). one hundred seventy-two rtcs were treated and subsequently underwent transplantation and followed up for at least one year and up to five years after transplantation. eighty-eight rtcs received vaccine, and 84 received placebo. results were analyzed according to the prevacci ... | 1988 | 2847687 |
| selective inhibition of human cytomegalovirus replication by a novel nucleoside, oxetanocin g. | a novel nucleoside with an oxetanosyl-n-glycoside has been recently isolated from a culture filtrate from bacillus megaterium and named oxetanocin a (n. shimada, s. hasegawa, t. harada, t. tomisawa, a. fujii, and t. takita, j. antibiot. 39:1623-1625, 1986). in this study, we evaluated the antiherpesvirus activity of oxetanocin a and its derivatives and found that 9-(2-deoxy-2-hydroxymethyl-beta-d-erythro-oxetanosyl)guanine (oxt-g) was very potent and selective in inhibiting the replication of hu ... | 1988 | 2847638 |
| hydrolysis of inositol lipids: an early signal of human cytomegalovirus infection. | the production of inositol 1,4,5-triphosphate (ip 3) and 1,2-diacylglycerol (dg) as an early cellular response to human cytomegalovirus (hcmv) infection was investigated in human embryo lung (lu) cells. the results obtained show transient 85 and 30% increases in ip3 and dg, respectively, 20 min post-exposure to hcmv. following heat inactivation or serum neutralization of hcmv stock or in the presence of papaverine we failed to detect increased levels of ip3 and dg relative to mock-infected lu cu ... | 1988 | 2845892 |
| synthesis and antiherpes virus activity of phosphate and phosphonate derivatives of 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine. | a series of phosphate esters of 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, 1) were synthesized and evaluated for antiherpes virus activity. the cyclic phosphate esters were made by a new, efficient method utilizing stannic chloride as a solubilizing agent. monophosphate 2 and bisphosphate 4 showed comparable activity to dhpg and probably acted as prodrugs of dhpg. on the other hand, the cyclic phosphate of dhpg 3 was taken up by cells and bypassed the virus-specified thymidine kinase. as ... | 1986 | 3009811 |
| immune responses to isolated human cytomegalovirus envelope proteins. | a group of envelope proteins of human cytomegalovirus, ga protein (l. pereira, m. hoffman, m. tatsuno, and d. dondero, virology 139:73-86, 1984; l. pereira, p. 383-404, in b. roizman, ed., the herpesviruses, vol. 3, 1985), and two protein mixtures (58,000-molecular-weight [58k]-66k and 130k-66k), separated by serial columns prepared with anti-ga immunoglobulin g from sera of immunized guinea pigs, induced neutralizing antibodies and a cellular immune response in the animals. the ga is a disulfid ... | 1986 | 3009877 |
| natural killing of fibroblasts infected with low-passage clinical isolates of human cytomegalovirus. | fibroblasts infected with most low-passage clinical isolates of human cytomegalovirus (cmv) were as susceptible to lysis by human natural killer (nk) cells as high passage ad-169-infected fibroblasts. nk lysis occurred despite the absence of detectable cmv-specific late membrane antigen(s) on the majority of the target cells infected with most of the low passage strains. the magnitude of nk lysis of different cmv-infected target cells did not correlate with their ability to induce ifn-alpha. nk ... | 1988 | 2844455 |
| comparative study with monospecific and monoclonal antibodies against a 65 k human cytomegalovirus protein. | immunofluorescence assay using monospecific and monoclonal antibodies to the 65 k major protein of human cytomegalovirus (hcmv) was carried out to monitor the expression of this protein in infected cells. regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immunofluorescent staining, all stained cytoplasmic inclusion bodies localized to the site of the hcmv-induced receptor for the fc portion of igg, suggesting that most of the 65 k major ... | 1988 | 2843150 |
| synthesis and antiviral activity of certain 4-substituted and 2,4-disubstituted 7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidines. | treatment of the sodium salt of 4-chloro-2-(methylthio)pyrrolo[2,3-d]pyrimidine (2) with (2-acetoxyethoxy)methyl bromide (3) has provided 4-chloro-2-(methylthio)-7[(2-acetoxyethoxy)methyl]pyrrolo[2,3- d]pyrimidine (4). ammonolysis of 4 at room temperature gave 4-chloro-2-(methylthio)-7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3- d]pyrimidine (5). however, ammonolysis of 5 at 130 degrees c furnished 4-amino-2-(methylthio)-7-[(2-hydroxyethoxy)methyl]-pyrrolo[2,3- d]pyrimidine (6), which on desulfurizati ... | 1988 | 2840500 |
| cytomegalovirus alters the von willebrand factor content in human endothelial cells. | cultured human endothelial cells were infected with human cytomegalovirus ad 169 and kerr. the infection resulted in the appearance of viral antigens in the nuclei of about 10% of the endothelial cells and in the concomitant disappearance of vwf from the infected cells. no differences were observed between endothelial cells from different sources (umbilical cord veins or arteries, adult veins). | 1988 | 2838925 |
| the use of dna probes in studies of human cytomegalovirus. | hybridization assays provide a sensitive and rapid means for studying the molecular biology of viral replication and for identifying viral nucleic acid in biological specimens. such assays are attractive because the detection of virus does not require intact virions or concomitant viral protein synthesis, both of which may be absent in a latently infected cell or in a virus-associated tumor. for molecular and clinical studies on human cytomegalovirus (hcmv), we have cloned and characterized subg ... | 1985 | 2992841 |
| monocyte-derived inhibitor of interleukin 1 induced by human cytomegalovirus. | it has previously been shown that human cytomegalovirus (hcmv) can exert immunosuppressive effects, and it has been suggested that these may be mediated by monocytes, although the mechanism is unclear. we showed that infection of human monocytes with the ad169 strain of hcmv abrogates their production of interleukin 1 (il-1) activity. this was associated with the release from infected monocytes of an inhibitor of il-1 activity which was also released after hcmv infection of the u937 macrophage-l ... | 1985 | 2991578 |
| inhibition of human cytomegalovirus by combined acyclovir and vidarabine. | the inhibition of human cytomegalovirus (hcmv) isolates by acyclovir (acv) and vidarabine (ara-a) was assessed by using an infectious-center plaque-reduction assay. when fixed concentrations of 4.5 micrograms of acv and 250 ng of ara-a per ml were compared singly and in combination, the viral inhibition resulting from the acv-ara-a combination was synergistic for three of four hcmv clinical isolates studied and additive for one hcmv isolate. an additional four hcmv strains obtained at postmortem ... | 1985 | 2988432 |
| cytomegalovirus dna in colorectal carcinoma tissues. | numerous studies had linked human cytomegalovirus (hcmv) infections with neoplasia. among various other malignant tumors, colonic carcinoma tissues were reported to contain dna sequences hybridizing with dna extracted from virus particles. gene technology allowed us to use a cloned viral dna library to measure hcmv in colorectal tumors more specifically. four of 38 tissue specimens did contain dna sequences homologous to cloned viral dna probes; however, in each of those cases, identical hybridi ... | 1985 | 2987605 |
| sequences in human cytomegalovirus which hybridize with the avian retrovirus oncogene v-myc are g + c rich and do not hybridize with the human c-myc gene. | the degree of relatedness between previously identified cross-hybridizing regions within human cytomegalovirus strain ad169 and the avian retrovirus oncogene v-myc were investigated by nucleotide sequence comparison. we found that the homologous regions between the human cytomegalovirus genome and v-myc are limited to short g + c-rich regions in each genome and that the human cytomegalovirus genome shares little or no homology with the human c-myc gene. | 1985 | 2993868 |
| abortive infection with human cytomegalovirus induces an alteration of growth pattern: morphological changes with cytocidal effect in rabbit kidney epithelial cells. brief report. | rabbit kidney epithelial cells (rk13) exhibited a cytopathic effect (cpe) characterized by cell rounding after infection with human cytomegalovirus (hcmv). although hcmv-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny virus could be observed in virus-infected rk13 cells. hcmv-infected rk13 cells showed a prolonged doubling time and a decreased saturation density in cell growth compared to uninfected ... | 1985 | 2994600 |
| antiprostate carcinoma monoclonal antibody (d83.21) cross reacts with a membrane antigen expressed on cytomegalovirus-transformed human fibroblasts. | virological and epidemiological studies have implicated human cytomegalovirus (hcmv) as a possible etiological agent of prostate cancer. because of the suspected associations, this laboratory tested the reactivity of a prostate-associated monoclonal antibody with hcmv-transformed cells. this mouse monoclonal antibody, d83.21, reacts with a membrane antigen on prostate and bladder tumor cells and does not bind to a variety of other malignant or normal cells. the results of this study indicated th ... | 1985 | 2983294 |
| demonstration of the colinearity of human cytomegalovirus genomes and construction of restriction maps of unknown isolates using cloned subgenomic fragments. | in this study, we have established the colinearity of human cytomegalovirus (hcmv) genomes using stringent conditions of dna-dna filter hybridization of hcmv hindiii fragments and cosmid-cloned ad169 strain hcmv dna fragments. large cosmid-cloned fragments of ad169 dna were used for the preparation of radioactive probes by nick translation. these probes were hybridized to hindiii digests of dna from three fresh isolates of hcmv and to that of the davis strain. using published hindiii restriction ... | 1985 | 2995554 |
| the selection and characterization of human monoclonal antibodies to human cytomegalovirus. | this communication describes the application of epstein-barr virus lymphocyte transformation technology to the production of human monoclonal antibodies specific for human cytomegalovirus. a group of such igg antibodies have been characterized in terms of subclass, light-chain composition, specificity for particular viral proteins and neutralizing capacity. these results have shown that the production of antibodies by transformed lymphocytes is representative of the in vivo human immune response ... | 1986 | 3021800 |
| molecular cloning and analysis of three cdna clones homologous to human cytomegalovirus rnas present during late infection. | three virus-specific clones were isolated from a cdna library synthesized from human cytomegalovirus (ad169)-infected cell rna and cloned into the expression vector lambda gt11. these clones, designated c3, d10, and h10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. by using the cloned cdna, we were able to identify the transcripts that code for each gene product and study the kinetics of exp ... | 1986 | 3021986 |
| immunological cross-reactivities among three herpesviruses. | sixty adults were tested for humoral and cell-mediated immunity to varicella zoster virus (vzv), type 1 herpes simplex virus (hsv-1) and the human cytomegalovirus (cmv). since herpesviruses share common antigens, we compared results in these individuals to assess whether our tests gave false positives due to cross-reactions. of the 60, igg antibody to vzv, hsv-1, cmv tested by elisa was detected in 51 (85%), 34 (57%) and 20 (33%) respectively. all possible permutations of results were obtained a ... | 1985 | 2997331 |
| detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. | in situ hybridization was used to detect human cytomegalovirus (hcmv) in the peripheral blood mononuclear cells of some naturally infected (seropositive) individuals. a subpopulation of cells hybridized specifically to a portion of the hcmv genome that is heavily transcribed during the immediate-early period of infection. the hybridization signal was markedly reduced by base hydrolysis and ribonuclease, and therefore the probe appears to be detecting viral rna. a fluorescence-activated cell sort ... | 1985 | 2997930 |
| [effect of tunicamycin on human cytomegalovirus replication]. | | 1985 | 2998078 |
| preparation of highly purified human cytomegalovirus envelope antigen. | a human cytomegalovirus (hcmv) envelope preparation was highly purified by immunoaffinity column chromatography using an anti-cellular-igg column. the purified envelope induced high titre antibodies to hcmv in guinea-pigs. analysis of the guinea-pig immune sera by ria and immunofluorescence (if) showed that this envelope preparation, unlike its unpurified counterpart, did not induce antibody to cellular contaminants. dot-blot assay revealed viral proteins in the flow-through fraction and cellula ... | 1985 | 2998113 |
| a murine monoclonal antibody recognising a single glycoprotein within a human cytomegalovirus virion envelope glycoprotein complex. | nonionic detergent solubilised polypeptides from highly purified human cytomegalovirus virions were used as immunogens to produce murine monoclonal antibody secreting hybridomas. one monoclonal antibody was shown, by immunoprecipitation followed by sds-polyacrylamide gel electrophoresis (sds-page), to precipitate three glycoproteins with molecular weights 52, 95, and 130 (all x 10(3)) and one minor component with a molecular weight of 50 x 10(3). when virion envelope components were first separa ... | 1985 | 2999324 |
| autoregulation of the human cytomegalovirus major immediate-early gene. | the gene coding for the human cytomegalovirus major immediate-early 72-kilodalton protein was cloned into simian virus origin of dna replication plasmid psvod. transfection of this plasmid (psvcc2) into cells constitutively expressing the simian virus 40 t-antigen resulted in readily detectable levels of immediate-early region 1-specific rna and protein. partial restriction enzyme digestion of psvcc2 was used to generate specific amino acid deletions within the 72-kilodalton protein. mutant delt ... | 1985 | 2999424 |
| a test for human cytomegalovirus-specific immunoglobulins using a modification of a commercial test kit. | a technique (ig-eia) for the detection of cmv-specific igg, igm and iga in human blood is described. ig-eia utilizes alkaline phosphatase-labeled goat anti-human igg, igm and iga as a detection probe and cmv antigen-coated solid phase from commercial kits. ig-eia is compared to indirect fluorescent assay (ifa) and indirect hemagglutination (iha) for sensitivity and specificity. on sequential samples of blood from a set of patients, ig-eia clearly demonstrated seroconversion in cmv-specific igg a ... | 1987 | 3031114 |
| killing of human cytomegalovirus-infected fibroblasts by antiviral antibody and complement. | complement-dependent cytolytic antibodies (cyab) to cytomegalovirus (cmv)-infected fibroblasts were detectable in acute- and convalescent-phase sera from renal allograft recipients (n = 44) and nonimmunocompromised patients (n = 14) with symptomatic cmv infection but not in sera from control donors (n = 75; p less than .001 by wilcoxon rank sum test). renal allograft recipients with secondary cmv infection had the highest levels of cyab activity. activity closely correlated with the serum antibo ... | 1986 | 3001193 |