| [blood pressure following experimental kidney injury in rabbits]. | | 1975 | 1100990 |
| complement activation in relation to protective function of antibodies to escherichia coli o antigens. | the relation between complement activation in an indirect hemolysis assay of e. coli o antibodies and their protective function in experimentally infected mice was analyzed. the antibody amounts and avidities were estimated in antisera and in igg and igm antibody fractions by the ammonium sulphate precipitation (asp) technique of farr. the protection obtained was due to both igg and igm antibodies and showed a close relation to the quantity of antibodies measured by the asp technique. no link be ... | 1975 | 1101368 |
| [the endotoxin from s-, r- and m-forms of enteropathogenic e. coli o 149: pyrogenicity, shwartzman-phenomenon and hypersensitivity (author's transl)]. | the authors are interested in elucidation of the role of endotoxin from s, r- and m-forms of e. coli o 149, and of the role of hypersensitivity in pathogenesis of colibacillosis. it was shown in this experiment that all s, r- and m-form of e. coli o149 possess endotoxin with almost the same pyrogenicity in normal rabbits. the pyrogenicity increases enormously in the hypersensitized rabbit. there were also not observed differences between endotoxins extracted from s, r, m-mutnants. all 3 endotoxi ... | 1975 | 1101577 |
| masking of peptidyl transferase activity in polyribosomes. | | 1975 | 1101832 |
| different effects of bacterial lipopolysaccharide on neonatal immunological tolerance to hsa in rabbits and chickens. | addition of bacterial lipopolysaccharide to hsa during repeated immunization of rabbits neonatally rendered tolerant to hsa led to anti-hsa antibody formation in 4 out of 5 animals. in all 6 tolerant animals immunized with hsa alone tolerance persisted after cessation of the immunization series, as shown by non-immune elimination of 125i-hsa. on the other hand, immunization with hsa together with different doses of lps did not increase anti-hsa antibody levels in tolerant and control chickens. | 1975 | 1104379 |
| mechanisms of endotoxin tolerance. the role of the spleen. | splenectomy markedly impaired the production of circulating anti-endotoxin antibodies during the initial 10 days after .v. administration of a boivin preparation of escherichia coli endotoxin (et) in both rabbit and man. increase in antibodies with secondary (flocculating and bactericidal) activities were virtually abolished, whereas increases in antibodies with primary (binding) activity were significantly reduced. on the basis of these findings, splenectomized rabbit and man were employed to t ... | 1975 | 1104660 |
| a new method for producing a chronic e. coli pyelonephritis in rabbits. | chronic pyelonephritis, especially that caused by the most common invader e. coli, corresponding satisfactorily to the pathophysiology of the human disease is difficult to reproduce. we have developed a pattern of infection with which we succeeded reliably in producing a chronic e. coli pyelonephritis. this was achieved by inserting a plastic catheter into the renal pelvis. a suspension of e. coli was injected via the catheter into the renal pelvis of 32 rabbits. this pattern of infection takes ... | 1975 | 1105218 |
| readenylation of polyadenylate-free globin messenger rna restores its stability in vivo. | using an atp:rna adenyltransferase from escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mrna from which the poly (a) segment had been previously removed. conditions for obtaining a homogenous reconstituted globin mrna preparation containing 30 adenylic residues per message molecule were determined. the reconstituted globin mrna was microinjected into xenopus laevis oocytes. its stability was very similar to that of native mrna. | 1975 | 1107033 |
| actin-like properties from escherichia coli: concept of cytotonus as the missing link between cell metabolism and the biological ion-exchange resin. | a protein fraction (a-l fraction) with characteristics reminiscent of muscle actin has been isolated from escherichia coli. the a-l fraction undergoes reversible aggregation under the same conditions in which actin is polymerized and depends primarily on potassium for its polymerization. this fraction, upon electrophoresis on acrylamide gels in the presence of sodium dodecyl sulfate, exhibits a distinct peak at the characteristic molecular weight of 45,000. passage of skeletal muscle myosin thro ... | 1976 | 1107311 |
| doublet frequencies in sequenced nucleic acids. | a doublet frequency count (set of frequencies of the 16 possible two-base sequences) can be calculated from the experimentally determined overall sequence of a nucleic acid. in this paper, a statistical methodology is developed for comparing such counts with random, with others of the same type or with doublet proportions found in whole dnas. the methods are applied to two major categories of sequenced rnas. it is found that vertebrate ribosomal and transfer rnas show significant differences fro ... | 1975 | 1107565 |
| content of elongation factor tu in escherichia coli. | the content of elongation factor tu in e. coli b has been determined both by radioimmune assay and by gdp binding. the two assays gave comparable results: cells growing at 2 doublings per hour contained about 8 molecules of tu per ribosome, whereas those growing at 0.22 doublings per hour contained about 14 molecules per ribosome. these levels resemble those reported for trna, in contrast with the 1:1 ratio of factor to ribosomes reported for elongation factors ts and g. | 1975 | 1108000 |
| strand-selective transcription of globin genes in rabbit erythroid cells and chromatin. | in order to investigate the symmetry of globin gene transcription, complementary rna (crna) was synthesized using rabbit globin complementary dna (cdna) as a template for escherichia coli dna-dependent rna polymerase (rna nucleotidyltransferase). the crna hybridized specifically to its own cdna template but not to sheep cdna, rabbit globin mrna, or poly(dt). hybridization studies with crna demonstrated that rna sequences transcribed from the dna strand complementary to the globin gene region (an ... | 1975 | 1108006 |
| cell-free transcription of mammalian chromatin. quantitative measurement of newly synthesized globin messenger rna sequences. | transcription of globin mrna sequences from rabbit marrow chromatin was detected by hybridization with globin complementary dna (cdna). the presence of newly synthesized rna in cdna-globin mrna hybrids isolated by cs2so4 density equilibrium centrifugation required the addition of both rabbit marrow chromatin and dna-dependent rna polymerase (escherichia coli) to the transcription reaction. no globin mrna sequences were detected in rna transcribed from rabbit liver chromatin or from rabbit marrow ... | 1975 | 1184580 |
| intraction of aminoacyl-trna synthetases with ribosomes and ribosomal subunits. | salt-washed ribosomes from rabbit reticulocytes stimulate seven partially purified aminoacyl-trna synthetases up to threefold: arginyl-, alanyl-, isoleucyl-, lysyl-, methionyl-, phenylalanyl- and valyl-trna synthetase. both isolated subunits but not total ribosomal rna show the stimulation. the increase in synthetase activity is also found in a highly purified escherichia coli system employing homogenous (e. coli)phenyl-alanyl-trna synthetase and (e. coli)- trnaphe. the biological significance o ... | 1976 | 1252498 |
| preparation and properties of a national reference endotoxin. | a large pool of refined endotoxin was prepared from escherichia coli o113 by extraction with hot acqueous phenol. it was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin ec. | 1976 | 1254700 |
| enterohemorrhagic escherichia coli o157:h7 possesses somatic (o) antigen identical with that of salmonella o301. | | 1992 | 1280180 |
| [the ultrastructural changes in the glomeruli and juxtaglomerular apparatus at different periods of endotoxic shock]. | it is shown that after endotoxin injection the ultrastructural changes in the glomeruli can favour development of the acute renal insufficiency. in the initial and intermediate periods of the endotoxin shock the granular and agranular forms of juxtaglomerular cells hyperfunction, respectively, are revealed as well as an increase of renin activity in plasma. at the stage of the late endotoxemia ultrastructural alterations are stabilized. the juxtaglomerular cells synthesize and accumulate secreto ... | 1992 | 1286692 |
| protective effect of re-lps antiserum on experimental multiple system organ failure. | this study examines the possible beneficial effect of re-lps (f515) antiserum on experimental multiple system organ failure (msof) in rabbits. the results showed that the plasma lps level was significantly decreased, and it took a shorter period to clear up lps in experimental than in control rabbits after receiving re-lps antiserum. pretreatment with antiserum can markedly improve the function of the liver, lungs, kidneys, blood and gastrointestinal tract. the msof incidence in the group of rab ... | 1992 | 1291201 |
| intestinal colonization & production of diarrhoea by enteroadherent-aggregative escherichia coli. | the ability of hep-2 cell adherent esch. coli of aggregative phenotype (ea-agg ec) to cause diarrhoea and to colonize the bowel of rabbits was studied. thirty six rabbits were challenged with one of three ea-agg ec strains (f23a; h766c and f17a-15, 3 and 3 rabbits respectively) or a control strain (k12-15 rabbits) in reversible ileal-tie in adult rabbit diarrhoea (ritard) model. the animals were sacrificed 72 h post challenge. severe diarrhoea occurred in greater number of f23a challenged rabbit ... | 1992 | 1291461 |
| [disseminated intravascular coagulation induced by endotoxin in rabbits: effect of treatment with t-pa and urokinase]. | to assess the therapeutic efficacy of agents capable of stimulating the fibrinolytic system, such as tissue plasminogen activator (t-pa) and urokinase (uk) on endotoxin-induced disseminated intravascular coagulation (dic) in the rabbit. | 1992 | 1293794 |
| coexpression of the cys e and cys m genes of salmonella typhimurium in mammalian cells: a step towards establishing cysteine biosynthesis in sheep by transgenesis. | the salmonella typhimurium genes for serine acetyltransferase (cys e) and o-acetylserine sulphydrylase b (cys m) were isolated and characterized in order to express these as transgenes in sheep to establish a cysteine biosynthesis pathway and, thereby, to achieve an increased rate of wool growth. comparison of the s. typhimurium and escherichia coli genes showed considerable homology, both at the nucleotide and amino acid sequence levels. the in vitro and in vivo expression studies showed that b ... | 1992 | 1301209 |
| characterization of human cytomegalovirus ul84 early gene and identification of its putative protein product. | the dna sequence and transcription pattern of human cytomegalovirus early gene ul84 were analyzed. this gene was mapped within a 2.6-kb psti fragment located between 0.534 and 0.545 map unit of the large unique segment of the human cytomegalovirus genome, which is adjacent to the pp65 and pp71 genes. a 2.0-kb mrna was transcribed from this region in the same leftward direction as the mrnas of the pp65 and pp71 genes. the message was first detected at 2.5 h postinfection and reached a maximal lev ... | 1992 | 1309892 |
| molecular cloning of human macrophage capping protein cdna. a unique member of the gelsolin/villin family expressed primarily in macrophages. | macrophage capping protein (mcp) is a ca(2+)-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. the human cdna for mcp has been cloned and sequenced. the derived amino acid sequence predicts a polypeptide of 38.4 kda. human mcp expressed in escherichia coli using a pet12a vector was functionally identical to the native protein purified from rabbit alveolar macrophages with respect to ca2+ sensitivity and ability to block mon ... | 1992 | 1322908 |
| expression of the epstein-barr virus latent membrane protein (lmp) in insect cells and detection of antibodies in human sera against this protein. | recombinant baculoviruses containing the complete lmp and truncated lmp genes were generated and high levels of the lmp proteins were expressed in spadoptera frugiperda insect cells. a specific rabbit antiserum directed against the n-terminal part of lmp was obtained by immunizing the rabbits with escherichia coli-expressed trpe-n-terminal part of lmp fusion protein. a total of 127 human sera were studied for their immune response to the recombinant full-length lmp. in immunofluorescence analysi ... | 1992 | 1326801 |
| antibodies against 70-kd heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins. | previously, we found that anti-ddded antibodies strongly inhibited in vivo nuclear transport of nuclear proteins and that these antibodies recognized a protein of 69 kd (p69) from rat liver nuclear envelopes that showed specific binding activities to the nuclear location sequences (nlss) of nucleoplasmin and sv-40 large t-antigen. here we identified this protein as the 70-kd heat shock cognate protein (hsc70) based on its mass, isoelectric point, cellular localization, and partial amino acid seq ... | 1992 | 1332978 |
| effects of steroids and immunosuppressive drugs on endotoxin-uveitis in rabbits. | anti-inflammatory actions of dexamethasone (dexa), cyclosporin a (csa) and rapamycin (rapa) were assessed on uveitis induced by intravitreal e-coli endotoxin (100ng) in rabbits at 24 hrs. in this model, endotoxin caused a breakdown of the blood-aqueous barrier (bab) and polymorphonuclear neutrophils (pmn) infiltration into the aqueous humor (ah) and iris-ciliary body (icb). intramuscular (i.m.) dexa (2mg/kg) but not topical dexa (0.1% 6 x daily) inhibited ah leukocytes and protein level. however ... | 1992 | 1336536 |
| [remember escherichia coli mutabile]. | | 1992 | 1340254 |
| inhibition of enteropathogenic escherichia coli adherence to hela cells by immune rabbit sera. | we have studied the effect of immune rabbit sera on the localized (la) and diffuse (da) adherence to hela cells of 10 enteropathogenic escherichia coli (epec) strains belonging to serogroups o55, o86, o111, o119, and o142. anti-la1 serum, obtained by rabbit immunization with an e. coli strain harboring a cloned dna fragment from an epec la plasmid, strongly inhibited the adherence of all serogroups but one (o142). similar results were obtained with anti-la2 serum, which is anti-o111 serum absorb ... | 1992 | 1342613 |
| the adherence of verocytotoxin-producing escherichia coli to rabbit intestinal cells. | verocytotoxin-producing escherichia coli (vtec) have been recognised recently as an important cause of human disease. the adherence of vtec to rabbit intestinal tract and the relationship between adherence and other virulence traits were studied. twenty clinical isolates of vtec (o157:h7 and other serotypes) and a control, commensal e. coli strain, were examined. bacteria were evaluated for the presence of surface fimbriae, plasmid profile and hybridisation with a 3.4 kb dna probe derived from t ... | 1992 | 1359147 |
| characteristics of haemolytic escherichia coli with particular reference to production of cytotoxic necrotizing factor type 1 (cnf1). | a total of 1,106 escherichia coli strains isolated in spain between 1986 and 1991 from extraintestinal infections and faeces of healthy controls were examined for production of alpha-haemolysin (hly). among strains causing urinary tract infections, sepsis and other extraintestinal infections, hly production was detected in 51% (p < 0.001), 32% (p < 0.001) and 18% (p < 0.02), respectively. in contrast, only 9% of faecal isolates from healthy individuals synthesized hly. the 356 haemolytic e. coli ... | 1992 | 1363736 |
| molecular and immunological characterization of adp-ribosylarginine hydrolases. | mono-adp-ribosylation is a reversible modification of proteins with nad:arginine adp-ribosyltransferases and adp-ribosylarginine hydrolases catalyzing the forward and reverse reactions, respectively. hydrolase activities were present in a variety of animal species, with the highest specific activities found in rat and mouse brain, spleen, and testis. rat and mouse hydrolases were dithiothreitol- and mg(2+)-dependent, whereas the bovine and guinea pig enzymes were dithiothreitol-independent. a ra ... | 1992 | 1375222 |
| synthetic recombinant vaccine expressing influenza haemagglutinin epitope in salmonella flagellin leads to partial protection in mice. | the influenza virus haemagglutinin epitope 91-108, which is a conserved amino acid sequence in all type a h3 strains, was expressed in salmonella flagellin, to evaluate its potential as a vaccine. for that purpose, a synthetic oligonucleotide comprising 54 bases coding for the corresponding sequence was inserted into the plasmid pls408 and transformed into escherichia coli jm101. colonies containing the recombinant plasmid were used to transform salmonella typhimurium lb5000 and were then transd ... | 1992 | 1376012 |
| molecular cloning, stage-specific expression and cellular distribution of a putative protein kinase from plasmodium falciparum. | a putative protein kinase gene (pfpk2) has been isolated from the human parasite plasmodium falciparum by using a mixed oligonucleotide pool which corresponds to a highly conserved region of serine/threonine protein kinases. the complete nucleotide sequence of 5 kb suggests the existence of a second transcriptional unit besides that of the pfpk2 gene, separated by a highly (a+t)-rich region and transcribed in a different orientation. no intron sequence exists in pfpk2. the predicted amino acid s ... | 1992 | 1378403 |
| location of the cytoplasmic epitope for a k(+)-competitive antibody of the (h+,k+)-atpase. | the monoclonal antibody (mab) 95-111 binds the alpha subunit of (h+,k+)-atpase and inhibits the k(+)-atpase activity. to map the epitope, all of the partial sequences of the alpha subunit were expressed in escherichia coli hb101 using rabbit alpha subunit cdna restriction fragments ligated into puex vector. bacterial recombinant lysates were separated by sodium dodecyl sulfate-gel electrophoresis, and the epitope was detected by western blotting. the antibody site was mapped between cys529 and g ... | 1992 | 1382067 |
| escherichia coli ribonucleotide reductase expression is cell cycle regulated. | the expression of the genes encoding ribonucleotide reductase in escherichia coli was investigated in cultures synchronized by obtaining the smallest cells in a population after sucrose gradient centrifugation. specific activity of ribonucleotide reductase and dna initiation were found to increase in parallel, periodically as a function of the cell cycle. the expression of nrd was also determined in cells synchronized by periodic repeated doubling in a phosphate limited medium. antibodies direct ... | 1992 | 1384814 |
| characterization of the reverse transcriptase of 1731, a drosophila melanogaster retrotransposon. | the nucleotide sequence of 1731, a retrotransposon cloned from the genome of drosophila melanogaster, reveals a structural similarity with the proviral form of the retroviruses including a pol-like gene containing a putative reverse-transcriptase(rt)-coding sequence. diverse parts of that sequence were subcloned and expressed in escherichia coli. it has been demonstrated that the expression of the rt-like sequence, when translated, gives rise to peptides displaying enzyme activity characteristic ... | 1992 | 1385119 |
| endothelium-accelerated hyporesponsiveness of norepinephrine-elicited contraction of rat aorta in the presence of bacterial lipopolysaccharide. | the role of the endothelium in the hyporesponsiveness of alpha-adrenoceptor-mediated contractions of the rat aorta was investigated. the norepinephrine-induced maximal contraction was diminished after repeated addition of the agonist. the hyporesponsiveness of the maximal contraction was endothelium dependent, being prevented by ng-monomethyl-l-arginine (0.5 mm), l-argininosuccinic acid (0.5 mm), puromycin (ic50 = 100 microm), actinomycin d (ic50 = 80 nm) but not by indomethacin, which suggests ... | 1992 | 1385173 |
| characterization of a ca2+ binding and regulatory site in the ca2+ release channel (ryanodine receptor) of rabbit skeletal muscle sarcoplasmic reticulum. | a region in the skeletal muscle ryanodine receptor between amino acids 4014 and 4765 was expressed as a trpe fusion protein. overlay studies revealed that this region bound ca2+ and ruthenium red, an indicator of ca(2+)-binding sites. ca2+ binding was mapped to subregion 13b between amino acids 4246 and 4377, encompassing a predicted high affinity ca(2+)-binding site, and to subregion 13c between amino acids 4364 and 4529, encompassing two predicted high affinity ca(2+)-binding sites. ca2+ bindi ... | 1992 | 1385418 |
| [ards: possible role of bacterial exotoxin]. | | 1992 | 1391370 |
| heterogeneity among heat-labile enterotoxins produced by porcine enterotoxigenic escherichia coli. | the heat-labile enterotoxins (lt) produced by various porcine strains (ltp) of enterotoxigenic escherichia coli were purified to homogeneity and their molecular properties were compared with each other. by double gel diffusion and rabbit skin permeability test, ltps from wt-1 (ltp-wt-1) and bo-149 (ltp-bo-149) strains were antigenically and biologically identical. by polyacrylamide gel electrophoresis with sodium dodecyl sulfate (sds), the mobilities of a and b subunits of ltp (bo-149) were iden ... | 1992 | 1397223 |
| identification of tumor necrosis factor as a transcriptional regulator of the phosphoenolpyruvate carboxykinase gene following endotoxin treatment of mice. | the decreased synthesis of hepatic phosphoenolpyruvate carboxykinase (pepck), the rate-limiting enzyme of gluconeogenesis, that occurs during endotoxemia was shown previously in rats to occur at the transcriptional level. in the current study, the exogenous administration of human recombinant tumor necrosis factor (tnf), a proximal mediator of endotoxic shock, reduced the pepck transcription rate, mrnapepck levels, and pepck enzyme activity in a time- and dose-dependent manner in cd-1 mice. comp ... | 1992 | 1398916 |
| experimental verocytotoxemia in rabbits. | the clinicopathologic effects of intravenously administered purified verocytotoxin 1 (vt1; shiga-like toxin 1) in 2-kg male rabbits was studied. the 50% lethal dose was 0.2 micrograms of protein per kg of body weight (2 x 10(4) 50% cytotoxic doses per kg). the clinical features included nonbloody diarrhea and a progressive flaccid paresis, usually culminating in death. the histopathology was characterized by edema and hemorrhage in the mucosa and submucosa of the cecum and edema, hemorrhage, and ... | 1992 | 1398926 |
| sensitivity to endotoxin in rabbits is increased after hemorrhagic shock. | the immunoinflammatory response following trauma and hemorrhage may predispose to the development of sepsis and multiple-organ failure syndrome. cardiac output (co), arterial pressure, arterial po2, and pulmonary permeability index were measured. we examined the sensitivity of rabbits to infusions of lipopolysaccharide (lps) after hemorrhagic shock. shock was produced by reducing co to 40% of baseline for 90 min, followed by resuscitation with shed blood and then with lactated ringer solution to ... | 1992 | 1400029 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| aqueous humor polyamines and alkaline phosphatase activity in endotoxin-induced uveitis: correlations to diverse leukocyte subsets. | the polyamines putrescine, spermidine and spermine have been proposed to be a part of the acute phase inflammatory response. they have been shown to be useful markers for cellular kinetics and change with various pathological conditions. the hypothesis that aqueous humor polyamines could be used to follow the time course of an endotoxin-induced inflammation in the eye was investigated. additional parameters studied were the amount of aqueous leukocytes, alkaline phosphatase activity, distributio ... | 1992 | 1407960 |
| bacterial single-chain antibody fragments, specific for carcinoembryonic antigen. | we have produced single-chain antibody (scfv) fragments in bacteria specific for carcinoembryonic antigen (cea). polymerase chain reaction (pcr) was used for the cloning and modification of the heavy and light variable regions (vh and vl) of the mouse monoclonal antibody (mab) cb-cea.1. a 14-amino acid linker was used in the synthesis of the scfv gene. the vh and vl regions were amplified from cdna by pcr using 5' end fr1 and 3' end constant region primers, and then sequenced. vh was then amplif ... | 1992 | 1418981 |
| construction of a high-copy "atg vector" for expression in escherichia coli. | we report the construction of an inducible, high-copy plasmid for the expression of foreign proteins in escherichia coli. this plasmid, ppb1, combines the trc promoter, beta-galactosidase translation start site, and polylinker of pkk233-2 with the origin of replication region of puc19. replacement of the origin of replication of pkk233-2 results in a threefold increase in plasmid copy number of ppb1 compared with pkk233-2. subclones of the cdna for rabbit muscle fructose-1,6-bisphosphate aldolas ... | 1992 | 1422227 |
| reveromycins, new inhibitors of eukaryotic cell growth. ii. biological activities. | reveromycins a, b, c and d showed inhibitory activity against egf-stimulated mitogen response in balb/mk cells. furthermore reveromycins a, c and d exhibited morphological reversion of srcts-nrk cells, antiproliferative activity against human tumor cell lines and antifungal activity. the effects of reveromycins a, c and d on eukaryotic cells were closely similar to each other, but those of reveromycin b were very weak. in vitro studies revealed that reveromycin a is a selective inhibitor of prot ... | 1992 | 1429226 |
| genotypic and phenotypic characterization of an arod deletion-attenuated escherichia coli k12-shigella flexneri hybrid vaccine expressing s. flexneri 2a somatic antigen. | the construction and characterization of ecsf2a-2, an arod-deleted escherichia coli-shigella hybrid vaccine carrying chromosomal and plasmid genes from shigella flexneri and expressing s. flexneri 2a somatic antigen in association with e. coli k12 core are described. expression of hybrid lipopolysaccharide and deletion of arod resulted in the attenuation of phenotypic characteristics associated with pathogenicity. the addition of an arod deletion results in a requirement for an aromatic precurso ... | 1992 | 1441732 |
| pharmacokinetics of 18f-labeled fleroxacin in rabbits with escherichia coli infections, studied with positron emission tomography. | 18f-labeled fleroxacin was used to measure the pharmacokinetics of fleroxacin in healthy and infected animals by positron emission tomography (pet) and tissue radioactivity measurements. in all experiments, a pharmacological dose of unlabeled drug (10 mg/kg) was coinjected with the tracer. the pharmacokinetics of [18f]fleroxacin was measured in groups of healthy mice (n = six per group) at 10, 30, 60, and 120 min after injection and in groups of rats with escherichia coli thigh infections (n = s ... | 1992 | 1444310 |
| nuclear localization and hepatic zonation of rat "spot 14" protein: immunohistochemical investigation employing anti-fusion protein antibodies. | s14 protein and mrna levels are rapidly regulated by hormones and diet. we have purified a 45-kd fusion protein from lysates of transformed e. coli that includes the entire s14 polypeptide. affinity-purified rabbit anti-fusion protein antibodies were used in immunohistochemistry to determine the distribution of s14 protein across the hepatic lobule, and to reassess its intracellular location. in hyperthyroid liver, s14 protein clustered near the central venous zone, and was not detectable in the ... | 1992 | 1446647 |
| identification and sequence of a na(+)-linked gene from the marine bacterium alteromonas haloplanktis which functionally complements the daga gene of escherichia coli. | a 4.0 kb fragment from a plasmid genomic dna library of the marine bacterium alteromonas haloplanktis atcc 19855 was found in the presence of na+ to complement the daga gene of escherichia coli. we have completely sequenced this fragment and the position of the na(+)-linked d-alanine glycine permease gene (daga) on the fragment has been determined by complementation. the predicted carrier protein consists of 542 amino acid residues (m(r) 58,955). its hydropathy profile suggests it is composed of ... | 1992 | 1447975 |
| ovine pulmonary surfactant induces killing of pasteurella haemolytica, escherichia coli, and klebsiella pneumoniae by normal serum. | pulmonary surfactant has been shown to play an increasingly important role in bacterial clearance at the alveolar surface in the lung. this study describes a bactericidal mechanism in which ovine pulmonary surfactant induces killing of pasteurella haemolytica by normal serum. to demonstrate killing, six bacterial species were incubated first with pulmonary surfactant for 60 min at 37 degrees c and then with serum for an additional 60 min at 37 degrees c. p. haemolytica type a1 strains 82-25 and ... | 1992 | 1452351 |
| salmonella serogroups c2 and c3 identified by agglutination using an immunoglobulin g3(kappa) monoclonal antibody (32-1-e3) reactive with a somatic factor 8-like polysaccharide antigen. | an immunoglobulin g3(kappa) monoclonal antibody (mab), mab 32-1-e3, which was prepared in balb/c mice by using a heated, alcohol-acetone-extracted salmonella newport cdc 50 antigen, reacted with protein-free lipopolysaccharides from salmonella groups c2 (o:6,8) and c3 (o:-,8) but not with those from any other serogroup tested. sodium periodate did not inhibit antigen reactivity, which was consistent with its identity as the abequose-containing disaccharide o:8 antigen. reactivity was inhibited b ... | 1992 | 1452684 |
| studies on processing, particle formation, and immunogenicity of the hiv-1 gag gene product: a possible component of a hiv vaccine. | antigens in a particulate conformation were shown to be highly immunogenic in mammals. for this reason, the particle forming capacity of derivatives of the hiv-1 group specific core antigen p55 gag was assayed and compared dependent on various expression systems: recombinant bacteria, vaccinia- and baculoviruses were established encoding the entire core protein p55 either in its authentic sequence or lacking the myristylation consensus signal. moreover, p55 gag was expressed in combination with ... | 1992 | 1456888 |
| site-directed mutation makes rabbit calcyclin dimer. | unlike human, rat and mouse calcyclin, purified rabbit calcyclin did not form a dimer on tricine sds-page under non-reduced conditions. based on the internal peptide sequence of rabbit calcylin, we isolated and sequenced a cdna clone encoding calcyclin. the sequence of this clone (pcalc) is 629 bp long and codes 90 amino acid residues of a protein with a molecular mass of 10,153 da. by northern blot analysis, a major band of 0.9 kbp and a minor band of 2.6 kbp were detected in the lung. the reco ... | 1992 | 1459239 |
| overexpression of a rhodococcus erythropolis protein in escherichia coli with immunological identity to the rhodococcus steroid 1-dehydrogenase. immunoelectron microscopic localization and electrophoretic studies. | the recombinant escherichia coli k-12 strain chi 6060 harbouring the plasmid pya 1201 with a gene from rhodococcus erythropolis imet 7030 overexpressed a protein which reacts with a monospecific antiserum against the steroid 1-dehydrogenase (sdh) from the same rhodococcus strain. it was shown previously that this recombinant protein exhibits no enzymatic activity. by immunogold labelling the protein was localized on ultrathin sections of the recombinant e. coli strain. after cultivation at 37 de ... | 1992 | 1460569 |
| chimeric myosin regulatory light chains identify the subdomain responsible for regulatory function. | regulatory light chains, located on the 'motor' head domains of myosin, belong to the family of ca2+ binding proteins that consist of four 'ef-hand' subdomains. vertebrate regulatory light chains can be divided into two functional classes: (i) in smooth/non-muscle myosins, phosphorylation of the light chains by a calcium/calmodulin-dependent kinase regulates both interaction of the myosin head with actin and assembly of the myosin into filaments, (ii) the light chains of skeletal muscle myosins ... | 1992 | 1464306 |
| a myxoma virus intergenic transient dominant selection vector. | the purpose of this study was to construct an intergenic transfer vector which can be used for the generation of recombinant myxoma viruses (mvs) expressing a foreign gene insert. recombinant mvs expressing the escherichia coli lacz gene were constructed in vitro by transfection of mv-infected rabbit cells with a transfer expression vector, and isolated under growth conditions selecting for transient expression of the e. coli gpt gene. the effect of inserting foreign dna sequences between the vi ... | 1992 | 1469364 |
| [protective effect of antiserum to re-lps on experimental multiple organ failure]. | we determined the possible beneficial effect of administering antiserum against re-lps(f515) on experimental multiple system organ failure (msof) in rabbits. the results showed that there were a more significant decrease of the plasma lps level and a shorter period to recovery than in the control group after receiving antiserum to re-lps. pretreatment with antiserum can remarkably improve the function of liver, lung, kidney, blood and gastrointestinal tract. the incidence of msof in rabbits rece ... | 1992 | 1473411 |
| a polyclonal antibody to the rat oestrogen receptor expressed in escherichia coli: characterization and application to immunohistochemistry. | a rat oestrogen receptor-beta-galactosidase fusion protein was expressed using a pex2/rat oestrogen receptor cdna construct. scatchard analysis of [3h]oestradiol-17 beta binding to the cell lysate revealed that the fusion protein had functional binding sites specific for oestradiol with a dissociation constant of 1.49 nmol/l. the relative molecular weight (m(r)) of the fusion protein was determined as 180,000 by immunoblot analysis of the cell lysate employing a monoclonal antibody to the human ... | 1992 | 1474341 |
| sequence homology and absence of mrna defines a possible pseudogene member of the trypanosoma cruzi gp85/sialidase multigene family. | a genomic clone, ptt21, containing dna apparently transcribed specifically in trypanosoma cruzi trypomastigotes, was obtained by differentially screening a genomic library with trypomastigote and epimastigote cdna. this 3444-bp clone contained open reading frames at each end, separated by a 1.8-kb non-coding region. the translated polypeptide from the 3' open reading frame (orf2) of 1037 bp had 25-30% identity with 5 recently published t. cruzi gp85/sialidase sequences, and 20-25% identity with ... | 1992 | 1474990 |
| effects of human intravenous immune globulin on diarrhea caused by shiga-like toxin i and shiga-like toxin ii in infant rabbits. | shiga toxin and the related shiga-like toxins (slt), produced by escherichia coli, can cause hemorrhagic colitis and hemolytic uremic syndrome (hus). human intravenous immune globulin (hivig) blocks the cytotoxicity of some slts in vitro. to examine the ability of hivig to modify disease caused by shiga-like toxin i or shiga-like toxin ii (slt-i or slt-ii), we injected 3-day-old rabbits intraperitoneally with slt-containing cell-free supernatants from escherichia coli o157: h7. a subset of rabbi ... | 1992 | 1479962 |
| effects of transcutaneous needle stimulation on the immune response in rabbits. | studies in the field of neuroimmunomodulation have shown that endorphins such as methionine-encephalin may have an immunomodulatory role. this study was undertaken to test whether transcutaneous needle stimulation, which is known to release such peptides from the central neurones, has an effect on immune response in rabbits. twelve healthy rabbits, in three similar subgroups, were exposed to pathogenic escherichia coli and their immune response was studied under different experimental conditions ... | 1992 | 1486646 |
| characterization of bep1 and bep4 antigens involved in cell interactions during paracentrotus lividus development. | we have identified and partially characterised two antigens, extracted with 3% butanol, from paracentrotus lividus embryos dissociated at the blastula stage, and encoded by the cdna clones previously described as bep1 and bep4 (bep-butanol extracted proteins). the cdna fragments containing the specific central portions of bep1 and bep4 were expressed as ms2 polymerase fusion proteins in escherichia coli. these two fusion proteins, called 1c1 (bep1) and 4a1 (bep4), were injected subcutaneously in ... | 1992 | 1499880 |
| purification and characterization of the hpr protein of pediococcus halophilus. | hpr protein, the ptsh gene product, of the phosphoenolpyruvate:sugar phosphotransferase system of pediococcus halophilus was purified to homogeneity using heat and acid treatments, deae-sepharose cl-6b and hydroxyapatite column chromatographies. the purified protein complemented the hpr activity of the phosphotransferase system in staphylococcus aureus ptsh mutant cell lysate. the molecular weight was estimated as 6,500 by sds polyacrylamide gel electrophoresis. the amino acid sequence of the am ... | 1992 | 1503564 |
| effects of bacterial endotoxin, interleukin-1 and prostaglandins on thermoregulation and cerebrospinal fluid-calcium concentration in rabbits. | | 1992 | 1508800 |
| salmonella typhi cell wall protein showing antigenic similarity to transferrin. | antiserum to cell wall proteins of s. typhi showed immunoprecipitin lines of identity with homologous antigen, normal human serum and human transferrin. also, anti-transferrin antibody showed immunoprecipitin band with s. typhi cell wall proteins and agglutinated s. typhi whole cells, but not eschericia coli. it is speculated that transferrin or a transferrin-like protein present in the cell wall may play a role in iron-uptake by s. typhi. | 1992 | 1512033 |
| mapping of immunodominant epitopes of the hiv-1 and hiv-2 integrase proteins by recombinant proteins and synthetic peptides. | different parts of the human immunodeficiency virus type 1 and type 2 (hiv-1 and hiv-2) integrase proteins were expressed as trpe fusion proteins in escherichia coli and used to screen human sera. in the immunoblot, all hiv/integrase-positive human sera tested reacted with the carboxy-terminal third of the integrase protein. furthermore, they crossreacted with the same part of the heterologous protein. half (50%) of the hiv-1/integrase-positive sera additionally detected antigenic epitopes in th ... | 1992 | 1520543 |
| cellular response to intravitreal injection of endotoxin and xanthine oxidase in rabbits. | in the present study, the ocular inflammatory response to intravitreally injected endotoxin and xanthine oxidase was studied and the cellular response of the anterior and posterior segments was contrasted. there was a clear dose response relationship to both compounds in aqueous humor protein concentration and aqueous and vitreous humor white cell number. xanthine oxidase and low doses of endotoxin (0.25 and 1.0 ng) produce a mainly mononuclear response in the anterior segment. higher doses of e ... | 1992 | 1521815 |
| association of the 90-kda heat shock protein does not affect the ligand-binding ability of androgen receptor. | an n-terminal truncated androgen receptor with putative dna- and ligand-binding domains (ar438) and that with a ligand-binding domain (ar612) were expressed under control of the t7 promoter in e. coli or translated in vitro with rabbit reticulocyte lysate, and their ligand-binding properties and the interaction with hsp90 were investigated. bacterially expressed ar438 and ar612 bound a synthetic androgen, [3h]r1881, with apparent dissociation constants of 2.6 +/- 0.2 and 3.1 +/- 0.7 nm, respecti ... | 1992 | 1525041 |
| immunochemical detection of group i and group ii phospholipases a2 in human serum. | time-resolved fluoroimmunoassay was developed for the detection of synovial-type phospholipase a2 (s-pla2) in human serum. this solid-phase, sandwich assay uses a polyclonal rabbit antibody raised against synovial-type group ii pla2 produced in escherichia coli. no cross-reactions were detected between s-pla2 and pla2 from human or porcine pancreas, human ascitic fluid, or bee or cobra venom. in healthy individuals, the average concentration of s-pla2 is 3.7 micrograms/l, with a 95% reference in ... | 1992 | 1526020 |
| overexpression of higher eukaryotic membrane proteins in bacteria. novel insights obtained with the liver mitochondrial proton/phosphate symporter. | in order to better understand why higher eukaryotic membrane proteins, in contrast to soluble proteins, are not readily expressed in escherichia coli, the gene encoding the liver mitochondrial phosphate transporter (h+/pi symporter) (ferreira, g. c., pratt, r. d., and pedersen, p. l. (1989) j. biol. chem. 264, 15628-15633), was subcloned into a plasmid (pfog402) containing the alkaline phosphatase promoter and leader sequence. although this system is highly efficient in overexpressing soluble mi ... | 1992 | 1531983 |
| characteristics and diffusion in the rabbit of a phage for escherichia coli 0103. attempts to use this phage for therapy. | a bacteriophage for escherichia coli 0103 was isolated during a study on e. coli diarrhoea in intensive breeding units of rabbits. the phage had an isometric head and a short tail and resembled coliphage n4 (podoviridae). it had a very narrow host range and seemed to be specific for serogroup 0103, suggesting that it might be used for preliminary identification of e. coli strains of this serogroup instead of the usual slide agglutination. in view of its possible use as a therapeutic phage, we in ... | 1992 | 1532681 |
| [ultrastructural features of changes in capillaries and atrial cardiomyocytes induced by endotoxin injection]. | the experiments on the rabbits and dogs have revealed that in the initial period of endotoxin shock the number of secretory granules in the atrial cardiomyocytes decreases and vascular permeability increases due to transendothelial micropinocytosis. in the intermediate period of endotoxin shock the ultrastructural alterations progress. the development of ultrastructural manifestations of endotoxemia greatly depends on the condition of endocrine heart function. | 1992 | 1535466 |
| synthesis of the biologically active beta-subunit of human nerve growth factor in escherichia coli. | the gene (ngfb) encoding the beta subunit of mature human nerve growth factor (hngfb) was subcloned into the pjla503 expression vector under the control of bacteriophage promoters pr and pl, and expressed in escherichia coli. the recombinant protein represented approximately 3% of the total cellular protein. biologically active hngfb was solubilized (0.2% total ngfb) and purified by cation-exchange chromatography and it yielded two bands on polyacrylamide-gel electrophoresis under nonreducing co ... | 1992 | 1537563 |
| neutrophil depletion protects against liver injury from bacterial endotoxin. | neutrophil (pmn) infiltration is an early occurrence in the liver after exposure to hepatotoxic doses of endotoxin lipopolysaccharide (lps). the purpose of this study was to test the hypothesis that pmns contribute to the pathogenesis of lps hepatotoxicity. the immunoglobulin fraction from serum of rabbits immunized with rat pmns (anti-pmn ig) was administered intravenously to rats 18 and 6 hours before exposure to an hepatotoxic dose of lps (escherichia coli 0128:b12). this protocol caused a gr ... | 1992 | 1538588 |
| induction of severe vascular leakage by low doses of escherichia coli hemolysin in perfused rabbit lungs. | s. aureus alpha-toxin and e. coli hemolysin (hly) represent two prototypes of pore-forming cytolysins. both are established virulence factors and have been implicated in the development of septic lung failure. low doses of these agents cause thromboxane-mediated vasoconstriction and edema formation in isolated perfused rabbit lungs. in a preceding investigation, we observed that alpha-toxin causes overt endothelial cell damage in these lungs, as demonstrable by electron microscopy (seeger w, bir ... | 1992 | 1538589 |
| cdna cloning and functional expression of the schistosoma mansoni protective antigen triose-phosphate isomerase. | m.1 monoclonal antibody has previously been shown to passively transfer partial resistance to schistosome infection within mice and to recognize a 28-kda antigen that has peptide sequence homology with triose-phosphate isomerase (tpi; d-glyceraldehyde-3-phosphate ketol-isomerase, ec 5.3.1.1). we have now isolated the complete coding dna for schistosoma mansoni tpi and confirmed that this cdna encodes the 28-kda antigen recognized by m.1. the predicted translation product has strong homology with ... | 1992 | 1542681 |
| anoxia induces phospholipase a2 activation in rabbit renal proximal tubules. | phospholipase a2 (pla2) activation during anoxic cell injury was determined by use of a variety of approaches in rabbit proximal renal tubules. arachidonic acid (aa) mass release increased from 4 +/- 1 (normoxia control) to 40 +/- 6 ng/mg protein after 20 min and 106 +/- 16 ng/mg protein after 40 min of anoxia. pla2 activity was measured by estimating the amount of sn-2 fatty acid released from either 14c-labeled escherichia coli membranes or [14c]phosphatidylethanolamine (pe) micelles incubated ... | 1992 | 1558154 |
| effect of cow's milk protein absorption on the anaphylactic and systemic immune responses of young rabbits during bacterial diarrhoea. | in vivo and in vitro studies recently showed that intestinal permeability to cow's milk proteins such as beta-lactoglobulin increased transiently in infant rabbits infected by the enteroadherent escherichia coli strain rdec-1 at weaning. the consequences of this enhanced permeability for local anaphylactic and/or systemic immune responses were studied in infected and age-matched control rabbits at weaning, given either water or cow's milk diluted in water in addition to their solid diet. the sys ... | 1992 | 1582701 |
| functional expression of the alpha-hemolysin of staphylococcus aureus in intact escherichia coli and in cell lysates. deletion of five c-terminal amino acids selectively impairs hemolytic activity. | the alpha-hemolysin gene from staphylococcus aureus, excluding the 5' region encoding the hydrophobic leader sequence, was amplified from genomic dna. the identity of the disputed c terminus has been confirmed and revisions made to the internal sequence. the hemolysin is expressed at high levels in escherichia coli and has been purified to homogeneity from this source. in addition, active [35s-met]alpha-hemolysin of high specific radioactivity can be generated in an e. coli transcription-transla ... | 1992 | 1587866 |
| biophysical studies of cytochromes b5 with amino acid substitutions in the membrane-binding domain. | | 1992 | 1600105 |
| interactions between yersinia enterocolitica and the host with special reference to virulence plasmid encoded adhesion and humoral immunity. | this thesis is based on 8 publications and a review of the literature. the aim was to summarize present knowledge on molecular components of yersinia enterocolitica involved in (i) adhesion, with special reference to plasmid encoded factors and (ii) induction of antibodies in the host, and the quantitation of these two phenomena. adhesion of y. enterocolitica was quantitatively studied by methods that measured binding to cultured epithelial cells, mucosal constituents immobilized on polystyrene, ... | 1992 | 1611921 |
| characterization of bovine bladder mucin fractions that inhibit escherichia coli adherence to the mucin deficient rabbit bladder. | we have previously shown that a dialyzed, lyophilized saline extract from bovine urothelium can restore the antiadherence activity of the rabbit bladder following mucin removal with 50% acetone. this report describes results of initial purification of antiadherence factor(s) from bovine bladder mucin and describes results of biochemical analysis in an attempt to elucidate possible mechanism of this antiadherence activity. separation performed by gel filtration (spectra gel aca 34, 20-350 kd rang ... | 1992 | 1613864 |
| mutualism based on stress: selective synthesis and phosphorylation of a stress protein by an intracellular symbiont. | the effects of a temperature shift-up and various metabolic inhibitors on the protein synthesis of an endosymbiont isolated from the pea aphid were studied. the syntheses of at least three major polypeptides were stimulated transiently immediately after a temperature shift-up, and treatment with ethanol and heavy metals (cd2+ and as2+). one of these proteins, the 63 kda heat-shock protein (63-kda hsp), was immunoprecipitated with antiserum raised against symbionin, which is selectively synthesiz ... | 1992 | 1618730 |
| the effect of cyclosporin a on cationized bovine serum albumin-induced nephropathy in nzw rabbits. | the effect of cyclosporin a (cya) was studied on the morphology and protein excretion of a rabbit chronic serum sickness nephritis using cationized bovine serum albumin (cbsa). one group of rabbits was given intravenous (i.v.) immunizing doses of cbsa and escherichia coli endotoxin. one week later, these animals began a 6-week i.v. injection schedule of cbsa only. a second group followed the same injection protocol, but was given intramuscular (i.m.) cya for 3 days prior to the immunizing dose o ... | 1992 | 1625057 |
| late effects of endotoxin on the accumulation and function of monocytes in rabbit lungs. | recent studies from our laboratory show that the lung contains a marginated pool of monocytes. the present study was designed to investigate monocyte accumulation in this pool 4 to 28 h after a single dose of endotoxin when the endotoxin had disappeared from the circulation. this was accomplished by administering a single intravenous dose of endotoxin (escherichia coli, 50 micrograms/rabbit) to unanesthetized animals (n = 6) and saline to controls (n = 5) at time 0. four hours after this dose of ... | 1992 | 1626802 |
| generation of specific antibodies against the rap1a, rap1b and rap2 small gtp-binding proteins. analysis of rap and ras proteins in membranes from mammalian cells. | specific antibodies against rap1a and rap1b small gtp-binding proteins were generated by immunization of rabbits with peptides derived from the c-terminus of the processed proteins. immunoblot analysis of membranes from several mammalian cell lines and human thrombocytes with affinity-purified antibodies against rap1a or rap1b demonstrated the presence of multiple immunoreactive proteins in the 22-23 kda range, although at strongly varying levels. whereas both proteins were present in substantia ... | 1992 | 1628649 |
| biochemical and biophysical characterization of human recombinant ige-binding protein, an s-type animal lectin. | ige-binding protein (epsilon bp) was originally identified by virtue of its affinity for ige. it is now known to be a beta-galactoside-binding lectin with the characteristic of an s-type carbohydrate recognition domain. the protein is composed of two domains: the amino-terminal domain consisting of tandem repeats and the carboxyl-terminal domain containing sequences shared by other s-type carbohydrate recognition domains. the amino-terminal domain also contains a number of potential recognition ... | 1992 | 1629216 |
| [experimental study of bacterial adherence to biliary tract in rabbits]. | adherence to host tissues and cell surfaces is an early step in infections. however nothing has been reported about bacterial adherence in biliary infections. the following experiment was carried out in rabbits with escherichia coli isolated from the bile of a patient with acute suppurative cholangitis. adhesion was observed by scanning electron microscopy. first, with cholecystic mucosa of normal, jaundice, acid (mucosa was injured with 0.1 n hydrochloric acid) and stone groups (stones were ins ... | 1992 | 1630438 |
| mapping of a region of dengue virus type-2 glycoprotein required for binding by a neutralizing monoclonal antibody. | envelope glycoprotein e of flaviviruses is exposed at the surface of the virion, and is responsible for eliciting a neutralizing antibody (ab) response, as well as protective immunity in the host. in this report, we describe a method for the fine mapping of a linear sequence of the e protein of dengue virus type-2 (den-2), recognized by a type-specific and neutralizing monoclonal ab (mab), 3h5. first, an escherichia coli expression vector containing a heat-inducible lambda pl promoter was used t ... | 1992 | 1634111 |
| lipopolysaccharide priming of alveolar macrophages for enhanced synthesis of prostanoids involves induction of a novel prostaglandin h synthase. | we report here that lipopolysaccharide (lps) priming of rabbit alveolar macrophages leads to amplified synthesis of prostanoids, at least in part, by induction of a novel prostaglandin h synthase (pgh synthase). rabbit alveolar macrophages were cultured with or without added lps derived from escherichia coli 0111:b4 for 4 h and then stimulated with opsonized zymosan (opz). lps priming of alveolar macrophages resulted in enhanced release of thromboxane (tx) upon stimulation with opz, when compare ... | 1992 | 1634505 |
| the signal for golgi retention of bovine beta 1,4-galactosyltransferase is in the transmembrane domain. | the expression and localization of bovine beta 1,4-galactosyltransferase (gal t) has been studied in mammalian cells transfected with gal t cdna constructs, and the role of the amino-terminal domains of gal t in golgi localization examined. here we demonstrate that the transmembrane (signal/anchor) domain of bovine gal t contains a positive golgi retention signal. bovine gal t was characterized in transfected cells with anti-bovine gal t antibodies, affinity-purified from a rabbit antiserum usin ... | 1992 | 1637374 |
| activation of mammalian dna ligase i through phosphorylation by casein kinase ii. | mammalian dna ligase i has been shown to be a phosphoprotein. dephosphorylation of purified dna ligase i causes inactivation, an effect dependent on the presence of the n-terminal region of the protein. expression of full-length human dna ligase i in escherichia coli yielded soluble but catalytically inactive enzyme whereas an n-terminally truncated form expressed activity. incubation of the full-length preparation from e. coli with purified casein kinase ii (ckii) resulted in phosphorylation of ... | 1992 | 1639065 |
| identification of enap-1, an antimicrobial peptide from equine neutrophils. | endogenous, cysteine-rich antimicrobial peptides known as defensins are prominent components of human, rabbit, and rat neutrophils, yet little is known about their occurrence in other mammalian species. although we did not detect mature (i.e., processed) defensins in equine neutrophil granules, we found that these granules contained small amounts of other cysteine-rich peptides with antimicrobial activity. one of these, enap-1, was purified by a combination of gel permeation and reversed-phase h ... | 1992 | 1639474 |
| biological activity of synthetic phosphonooxyethyl analogs of lipid a and lipid a partial structures. | we investigated the biological activity of four new synthetic analogs of lipid a, termed pe-1, pe-2, pe-3, and pe-4. all compounds contain an alpha-oxyethyl-linked (-o-ch2-ch2-) phosphoryl group in position 1 of the reducing glucosaminyl residue (glcn i) of lipid a. pe-1 is a hexaacylated analog of escherichia coli lipid a (compound 506). pe-2 differs from pe-1 in carrying two myristic acid residues at glcn i. pe-3 has the same acylation pattern as pe-2, but glcn i is present in the beta anomeri ... | 1992 | 1639498 |
| activity of recombinant mitogillin and mitogillin immunoconjugates. | a synthetic gene for the aspergillus protein toxin mitogillin has been synthesized and expressed in escherichia coli. the recombinant mitogillin is a potent inhibitor of protein synthesis in vitro with an ic50 of 9.7 pm. immunoconjugates of recombinant mitogillin derivatized with s-acetylmercaptosuccinic anhydride and 5-methyl-2-iminothiolane modified h65 antibody kill t cell lines and peripheral blood mononuclear cells expressing the human cd5 surface antigen. native mitogillin contains 4 cyste ... | 1992 | 1644844 |
| enhanced recombination associated with the presence of insertion and deletion mutations in poxvirus-infected cells. | genetic crosses were performed in cells infected by shope fibroma virus in order to determine whether poxviral recombination is influenced by the types of mutations participating in a cross. infected cells were cotransfected with marked bacteriophage lambda dnas and, after dna recovery, the substrates were packaged into infectious particles using in vitro packaging extracts. this made it possible to analyze the products of poxviral recombination systems using lambda mutants plated on escherichia ... | 1991 | 1651592 |
| genomic structure and expression of human guanosine monophosphate reductase. | in vitro translation in the rabbit reticulocyte system and transient expression in cos7 cells were performed to characterize the protein encoded by a chromosome 6-linked human cdna clone, whose nucleotide sequence is homologous to that of escherichia coli guanosine monophosphate reductase (gmp reductase) cdna. the molecular weight of the peptide produced by the cdna was about 37,000 dalton, and the protein produced in the cos7 cells exhibited gmp reductase activity, substantiating that the cdna ... | 1991 | 1661705 |