| monoclonal antibody analysis of bovine herpesvirus-1 glycoprotein antigenic areas relevant to natural infection. | neutralizing antigenic areas on the glycoproteins of bovine herpesvirus-1 (bhv-1) were identified by reciprocal competition radioimmunoassays using monoclonal antibodies. three interrelated and two independent antigenic areas were identified on the 77-kda (k) giv envelope glycoprotein. antigenic analysis of this protein has not been previously described. four interrelated and one independent antigenic areas were found on the 97k giii envelope glycoprotein. a third group of monoclonal antibodies ... | 1988 | 2457277 |
| association of bovine respiratory syncytial virus with atypical interstitial pneumonia in feedlot cattle. | thirty-three cattle with fatal respiratory tract disease were examined for gross and histologic lesions and for the presence of viral and bacterial agents in the lungs. fifteen cattle had lesions characteristic of atypical interstitial pneumonia (aip), and 18 had other respiratory tract diseases, including infectious bovine rhinotracheitis, shipping fever pneumonia, bronchopneumonia, pulmonary abscess, and edema of the trachea. gross necropsy findings in the cattle with aip were uncollapsed and ... | 1988 | 2458690 |
| combined effects of fasting and diet on interferon production and virus replication in calves infected with a vaccine strain of infectious bovine rhinotracheitis virus. | a study was undertaken to investigate the combined effects of fasting and different diets on interferon (ifn) production and virus replication measured in nasal secretions of calves inoculated with a vaccine strain of infectious bovine rhinotracheitis virus. four groups of calves were inoculated intranasally with infectious bovine rhinotracheitis virus. two groups were inoculated 24 hours after onset of a 3-day fast; upon refeeding, 1 group was fed a maintenance diet (m diet) of hay, and the oth ... | 1988 | 2459999 |
| passively administered neutralizing monoclonal antibodies do not protect calves against bovine herpesvirus 1 infection. | monoclonal antibodies specific for defined epitopes on the gi, giii and giv envelope glycoproteins of bhv-1 were used individually or in glycoprotein-monospecific pools for passive immunization of young calves. although serum antibody titres comparable to those found in naturally infected and recovered calves were achieved, passive immunization failed to prevent the growth of bhv-1 in nasal and ocular mucosa and did not decrease the duration of viral shedding. | 1988 | 2461005 |
| epitope specificity and protective efficacy of the bovine immune response to bovine herpesvirus-1 glycoprotein vaccines. | bovine herpesvirus-1 (bhv-1) envelope glycoproteins gi, giii and giv were individually purified on monoclonal antibody affinity columns and injected intradermally into bhv-1 seronegative calves. the calves developed serum neutralizing antibodies that monospecifically precipitated the immunizing glycoprotein from a preparation of 125i-labelled bhv-1 envelope proteins. a competitive radioimmunoassay using the bovine antisera demonstrated that known functional epitopes had been retained in the glyc ... | 1988 | 2461006 |
| functional and topographical analyses of epitopes on bovine herpesvirus type 1 glycoprotein iv. | bovine herpesvirus type 1 (bhv-1) glycoprotein giv was purified by affinity chromatography. purified preparations showed two distinct components of 71 k and 140 k following electrophoresis in sodium dodecyl sulphate polyacrylamide gels. the polypeptides were separated, excised from the gel and used to immunize rabbits; the resulting antisera showed a high degree of cross reactivity indicating that these polypeptides represent monomeric and dimeric forms of the same glycoprotein. purified giv was ... | 1988 | 2463822 |
| characterization of anti-idiotype reagents to bovine herpesvirus-1 monoclonal antibody. | a monoclonal antibody (mab) to a neutralization epitope on the 97-kd glycoprotein of bovine herpesvirus-1 (bhv-1) was used to prepare an anti-idiotypic antibody in rabbits. purified f(ab')2 fragments of the mab were used to immunize the animals and the sera containing the greatest anti-idiotype activity were identified by elisa. after digestion of the immunoglobulins with pepsin and purification by affinity chromatography, anti-idiotype f(ab')2 fragments reacted specifically with the mab in elis ... | 1988 | 2467429 |
| epitope specificity of the bovine antibody response to the giii glycoprotein of bovine herpesvirus type 1. | a panel of monoclonal antibodies (mabs) to bhv-1, specific for gi and giii glycoproteins and for a nonglycosylated core protein p100, was used to examine the epitope specificity of the immune response to the virus in naturally exposed and experimentally infected cattle. sera from experimentally infected calves were analyzed in a competition elisa (c-elisa). antibody to an epitope on giii appeared as early as 4 days post infection, although virus-neutralizing antibody did not appear until day 8 o ... | 1989 | 2476147 |
| effects of bovine respiratory disease viruses and isoprinosine on bovine leukocyte function in vitro. | peripheral blood mononuclear cells obtained from 4- to 6-month-old-calves were inoculated in vitro with bovine herpesvirus-1, parainfluenza-3, or bovine virus diarrhea viruses. no increase in infectious virus progeny was observed; however, the viruses were detected in the cells for at least 96 h post-infection without any significant reduction in cell viability. the three viruses, either alone or in combination, suppressed phytohemagglutinin-induced proliferation of the mononuclear cells. the gr ... | 1989 | 2477940 |
| [the effectiveness of the paramunity inducer baypamun (pind-orf) for the prevention and metaphylaxis of an experimental infection with the infectious bovine rhinotracheitis virus in cattle]. | efficacy of the paramunity inducer baypamun (pind-orf) was evaluated by an ibr challenge trial in cattle, as one model for infectious diseases in bovine. prophylactic treatment with baypamun protected cattle against manifestation of clinical symptoms after experimental ibr infection. the degree of protection depended on the time between paramunization and challenge infection. even in metaphylactically paramunized cattle baypamun reduced the ibr symptoms. in correlation to the reduction of clinic ... | 1989 | 2479119 |
| effects of formalin inactivation on bovine herpes virus-1 glycoproteins and antibody response elicited by formalin-inactivated vaccines in rabbits. | the possibility of using a radioimmune assay (ria) to measure the retention of antigenic determinants on gi, giii, and giv, the three major bovine herpes virus 1 (bhv-1) glycoproteins, during the formalin inactivation and aluminium hydroxide adsorption commonly used in the manufacture of killed virus vaccines was investigated. monoclonal antibodies were used to measure thirteen previously identified glycoprotein epitopes in experimental vaccines containing live bhv-1 or formalin-killed bhv-1 and ... | 1989 | 2481909 |
| the interferon sensitivity of selected porcine viruses. | the objective of this study was to compare the sensitivity of 11 porcine viruses to the antiviral effects of porcine interferon-alpha in serum from piglets which had been infected 19 h previously with transmissible gastroenteritis virus, and of porcine interferon-beta prepared in pk-15 cells by induction with polyinosinic:polycytidylic acid, in yield reduction assays in pig kidney cells which were treated with interferon before virus challenge, and both before and after virus challenge. the most ... | 1989 | 2492445 |
| a farming systems study of abortion in dairy cattle on the atherton tableland. 2. the pattern of infectious diseases. | the role of infectious agents on dairy farms on the atherton tableland in tropical north queensland was studied as part of a comprehensive investigation into the causes of bovine abortion. the prevalence of antibody in serums collected from 7 herds whose annual abortion rates ranged from 3% to 21% were as follows: leptospira hardjo 49.9% (426/853), l. pomona 0.4% (3/851), bovine virus diarrhoea (bvd) 33.7% (35/104). infectious bovine rhinotracheitis virus (ibr) 11.5% (12/105), akabane virus 92.2 ... | 1989 | 2505741 |
| technical improvements of a commercial elisa to detect antibodies against bovine herpesvirus 1. | prevention and control of the spread of ibr/ipv virus in cattle presumes exact diagnosis even with large sample collections. the requirements are fulfilled firstly by the application of the most sensitive testing methods for virus specific antibodies, such as the enzyme-linked immunosorbent assay (elisa), and secondly by the introduction of bovine milk as a specimen for sampling. data are presented demonstrating the application of a commercial elisa system for evaluation of large sample collecti ... | 1989 | 2535887 |
| bovine herpesvirus-1 and parainfluenza-3 virus interactions: clinical and immunological response in calves. | calves infected with bovine herpesvirus-1 (bhv-1) or both bhv-1 and parainfluenza-3 virus (piv-3) developed clinical signs including fever, cough, and nasal and ocular discharges. animals infected with both viruses appeared more depressed and showed higher rectal temperature, while calves inoculated with piv-3 alone had a very mild clinical disease. both bhv-1 and piv-3 were recovered from nasal secretions up to six to eight days postinoculation. however, the virus titers were lower in calves wi ... | 1989 | 2536581 |
| inhibition of t-lymphocyte mitogenic responses and effects on cell functions by bovine herpesvirus 1. | the mitogenic response of bovine peripheral blood mononuclear cells stimulated by concanavalin a (cona) was suppressed by infectious bovine herpesvirus 1 (bhv-1). proliferation in response to interleukin-2 (il-2) by il-2-dependent lymphocyte cultures was also inhibited by bhv-1. although inhibition of mitogenesis approached 100%, less than 1 cell in 1,000 was productively infected by bhv-1 in cona-stimulated cultures. neither conditioned medium from mitogen-stimulated peripheral blood mononuclea ... | 1989 | 2538643 |
| characterization of bovine mononuclear cell populations with natural cytolytic activity against bovine herpesvirus 1-infected cells. | freshly isolated or overnight cultured peripheral blood mononuclear cells from immune or nonimmune animals had natural cytolytic activity against bovine herpesvirus 1 (bhv-1)-infected tumor target cells. no lysis was demonstrated against tumor target cells alone. this natural cytolytic activity was present in mononuclear cells from the spleen, lymph node, and peripheral blood but little or no cytolytic activity was detected in bone marrow or thymus cells. when monoclonal antibodies and complemen ... | 1989 | 2539267 |
| role of interferon-gamma in inducing cytotoxicity of peripheral blood mononuclear leukocytes to bovine herpesvirus type 1 (bhv-1)-infected cells. | in the present study, the possible role of interferon (ifn)-gamma on the induction of cytotoxic activity of peripheral blood mononuclear leukocytes (pbml) from bhv-1-immune cattle was investigated. supernatants obtained from bhv-1-immune pbml, stimulated under conditions similar to those required to demonstrate cytotoxicity, contained an antiviral substance capable of inducing 2'-5'-oligoadenylate synthetase activity in mdbk cells and mhc class ii antigen expression on epithelial cells. these su ... | 1989 | 2539268 |
| a neutrophil-derived antiviral protein: induction requirements and biological properties. | polymorphonuclear neutrophilic granulocytes (pmn) have been implicated as playing a role in antiviral defense. in addition to having phagocytic and cytotoxic activities, pmn may produce an antiviral substance with interferon (ifn)-like activity. the product, for which the name polyferon (pf) has been coined, is produced upon direct encounter of pmn with bovine herpesvirus 1 (bhv-1)-infected bovine cells or membranes thereof. exposure to purified virus only does not induce pf. the intimate intera ... | 1989 | 2539494 |
| synthesis, cellular location, and immunogenicity of bovine herpesvirus 1 glycoproteins gi and giii expressed by recombinant vaccinia virus. | two of the major glycoproteins of bovine herpesvirus 1 (bhv-1) are gi, a polypeptide complex with apparent molecular weights of 130,000, 74,000, and 55,000, and giii (a 91,000-molecular-weight [91k] glycoprotein), which also exists as a 180k dimer. vaccinia virus (vac) recombinants were constructed which carry full-length gi (vac-i) or giii (vac-iii) genes. the genes for gi and giii were each placed under the control of the early vac 7.5k gene promoter and inserted within the vac gene for thymid ... | 1989 | 2539509 |
| rapid detection of bovid herpes virus 1 antigens by counter immunoelectrophoresis and agar-gel immunodiffusion. | using hyperimmune rabbit sera to bhv1, id and ciep tests detected one antigen in bhv1 infected cell culture harvests. in nasal and ocular secretions collected from experimentally infected cattle during the early course of clinical disease two and one bhv1 antigens were detected by the id and ciep tests respectively. in specimens collected from eight cattle for 10 days after the onset of pyrexia the id test demonstrated precipitating antigens in 22/35 ocular secretions and 43/60 nasal secretions, ... | 1989 | 2540574 |
| infertility in heifers inoculated with modified-live bovine herpesvirus-1 vaccinal strains against infectious bovine rhinotracheitis on postbreeding day 14. | heifers were inoculated iv with 1 of 4 modified-live bovine herpesvirus-1 vaccinal strains against infectious bovine rhinotracheitis (2 heifers/strain) on postbreeding day (pbd) 14. the effect of infection on fertility was monitored by plasma progesterone assay at 1- to 3-day intervals from the time of virus exposure until pbd 60. infertility was detected in 4 of 8 inoculated heifers. in 2 heifers, progesterone concentrations decreased to values indicative of estrus within 10 days after inoculat ... | 1989 | 2540687 |
| bovine naturally cytolytic cell activation against bovine herpes virus type 1-infected cells does not require late viral glycoproteins. | freshly isolated or overnight cultured bovine peripheral blood mononuclear (pbm) cells lysed bovine herpes virus 1 (bhv-1)-infected allogeneic and xenogeneic target cells but not non-infected target cells. to determine if late viral genes contribute to target cell lysis, phosphonoacetic acid (paa), an inhibitor of dna polymerase activity, was used to block dna replication that is required for expression of late viral proteins. both adherent and non-adherent (na) cell populations mediated lysis a ... | 1989 | 2541073 |
| serological evidence of bovine herpesvirus 1-related virus infection in the white-tailed deer population on anticosti island, quebec. | high white-tailed deer (odocoileus virginianus) population densities and the occurrence of harsh environmental conditions are present on anticosti island, located in the gulf of saint-lawrence (quebec, canada). this island is the northernmost region of white-tailed deer distribution in northeastern north america. the aim of this work was to determine whether a herpesvirus serologically related to bovine herpesvirus 1 (bhv1) may occur in a stressed white-tailed deer population. one hundred one de ... | 1989 | 2541261 |
| efficacy of viral components of a nonabortigenic combination vaccine for prevention of respiratory and reproductive system diseases in cattle. | efficacy and safety of components of an im-administered vaccine for prevention of infectious bovine rhinotracheitis virus (ibrv), parainfluenza type-3 (pi-3) virus, bovine viral diarrhea virus (bvdv), and respiratory syncytial virus (rsv) infections and campylobacteriosis and leptospirosis were evaluated in cattle, including calves and pregnant cows. challenge of immunity tests were conducted in calves for ibrv, pi-3 virus, or bvdv vaccinal components. all inoculated calves developed serum-neutr ... | 1989 | 2542202 |
| detection of bovine herpesvirus type 1 via an immunoperoxidase method, using monoclonal antibodies. | the sensitivity and specificity of the immunoperoxidase technique, using monoclonal antibodies, for the detection of bovine herpesvirus type 1 (bhv-1) was assessed and compared with viral isolation methods. in this study, bhv-1 antigens were detected in impression smears of brain obtained from calves in which bhv-1 was isolated. false-positive results were not observed after double-blind examination. preliminary identification of isolates as antigenic variants was possible by use of 3 monoclonal ... | 1989 | 2543238 |
| temperature-sensitive mutants of bovine herpesvirus type 1: mutants which make unaltered levels of 'early' glycoproteins but fail to synthesize a 'late' glycoprotein. | the major glycoproteins of bovine herpesvirus type 1 showed distinct temporal patterns of expression. the glycoproteins gvp 11 and gvp 6 as well as its cleavage products, gvp 11a and gvp 16, were expressed early in the infectious process, whereas gvp 9 was expressed late. temperature-sensitive mutants were developed and characterized. mutants belonging to two complementation groups were unable to synthesize dna at 40 degrees c, the non-permissive temperature. in cells infected with these mutants ... | 1989 | 2543735 |
| analysis of the bovine herpesvirus type 1 thymidine kinase (tk) gene from wild-type virus and tk-deficient mutants. | five thymidine kinase (tk)-deficient mutants (b1 to b5) of bovine herpesvirus type 1 (bhv-1) were isolated by selection for resistance to the nucleotide analogue bromovinyldeoxyuridine. the genetic lesion in mutant b1 was localized in a 2.7 kb sali-sali subfragment (ftk2.7) which maps between 0.456 and 0.475 within the hindiii a fragment of the bhv-1 genome. the tk genes from wild-type and the tk-mutants b1 to b5 were cloned and sequenced using eight unique synthetic primers designed from a publ ... | 1989 | 2543766 |
| the effect of bovine herpesvirus type 1 glycoproteins gi and giii on herpesvirus infections. | we expressed the bovine herpesvirus type 1 (bhv-1) glycoproteins, gi and giii, in bovine cells using a bovine papillomavirus vector. the proteins expressed by these cells had the same mr as the native bhv-1 proteins and monoclonal antibodies detected no differences in their antigenic structure. cells expressing gi were infected with either bhv-1 or herpes simplex virus type 1 (hsv-1). the number of plaques in gi-expressing cells was similar to that seen with normal fibroblasts infected with bhv- ... | 1989 | 2543789 |
| [serologic and virologic studies of selected cattle with antibodies to bovine herpesviruses]. | in order to investigate the specificity of low titer antibodies to bhv 1, twelve cattle were subjected to stress and dexamethasone treatment. they were monitored virologically by inoculating cell cultures with naso-pharyngeal-, ocular- and vaginal- or preputial swabs and serologically by assessing the prevalence and incidence of antibodies to bovine, caprine-, porcine-, and equine herpesviruses and to bovine leukemia virus. antibodies were classified as specific for bhv 1 if the animals excreted ... | 1989 | 2544992 |
| production and characterization of monoclonal antibodies to bovid herpesvirus-4. | thirty-five hybridoma cell lines secreting monoclonal antibodies (mabs) against bovid herpesvirus-4 (bhv-4) strain v. test were produced. these hybrid cells resulted from the fusion of sp2/0 myeloma cells with splenocytes of balb/c mice previously immunized with purified bhv-4. a modified indirect fluorescent antibody test (ifat) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. the selected mabs were tested by the same ifat against a panel ... | 1989 | 2546320 |
| aujeszky's disease elisa: cross-reactions with other herpesvirus antisera. | the production of antibodies in pigs to 11 herpesviruses was investigated in relation to their ability to cross-react with aujeszky's disease virus (suid herpesvirus 1--shv1). of the herpesviruses tested only two, sheep herpesvirus (caprine herpesvirus 1) and dog herpesvirus (canid herpesvirus 1), failed to produce homologous virus antibodies. only the antibodies to bovine herpesvirus 1 (bhv1) produced a cross-reaction by shv1 enzyme-linked immunosorbent assay (elisa). no shv1 neutralizing antib ... | 1989 | 2548320 |
| transmission of a vaccinal strain of infectious bovine rhinotracheitis virus from intranasally vaccinated steers commingled with nonvaccinated steers. | ninety-seven feeder steers, averaging 7 months of age, were allotted to 3 groups. group i (n = 33) was vaccinated intranasally with an infectious bovine rhinotracheitis virus (ibrv) vaccine on postinoculation day (pid) 0; group ii (n = 31) was not vaccinated on pid 0 but was commingled with group i; and group iii (n = 33) served as controls housed in the same facility, but was physically separated from groups i and ii. on pid 20, all steers were given a modified-live ibrv vaccine im. virus isola ... | 1989 | 2548418 |
| the serological response to foot-and-mouth disease vaccination is not affected by simultaneous infectious bovine rhinotracheitis/adenovirus/parainfluenza 3 vaccination. | young calves were simultaneously vaccinated by subcutaneous route against foot-and-mouth disease (fmd) and against infectious bovine rhinotracheitis/adenovirus/parainfluenza-3 (ibr/adeno/pi-3) by intranasal route. the serological response against the 3 fmd virus types of the fmd vaccine was clearly positive. there was no significant difference between results of simultaneous fmd and ibr/adeno/pi-3 vaccination and fmd vaccination only. | 1989 | 2549906 |
| changes in the bovine herpesvirus 1 genome during acute infection, after reactivation from latency, and after superinfection in the host animal. | three subtypes, as defined by hindiii restriction endonuclease (re) analysis patterns, of bovine herpesvirus 1 (bhv 1) were used to inoculate seronegative, bhv 1-free cattle. these included: infectious bovine rhinotracheitis virus (ibrv), subtype 1.1; infectious pustular vulvovaginitis virus (ippv) isolate k22, subtype 1.2b; and ipvv isolate fi, subtype 1.2a. nasal, vaginal, and buffy coat samples were taken for virus isolation from each animal. re analysis was done on virus isolates collected d ... | 1989 | 2549919 |
| in vitro assessment of viral antigen content in inactivated aluminum hydroxide adjuvanted vaccines. | antigen capture enzyme immunoassays (elisa) were developed to assess the antigenic content of inactivated aluminum hydroxide (ah) adjuvanted porcine parvovirus, pseudorabies, and infectious bovine rhinotracheitis vaccines. reference preparations of these viruses were constructed as a basis for comparison. because ah-associated elisa interference was largely circumvented, the need for isotopic or complex antigen-adjuvant desorption methods was eliminated. a 4-parameter logistic model related opti ... | 1989 | 2550498 |
| infection of the middle nasal meatus of calves with pasteurella haemolytica serotype 1. | eight healthy nonstressed calves were inoculated with pasteurella haemolytica serotype 1, by instilling a broth culture into the middle nasal meatus of the left nostril. the inoculated left nostrils shed p haemolytica from the ventral nasal meatus at a steady rate for a mean of 7 days, whereas the uninoculated right nostrils of the same calves shed p haemolytica sporadically and in lower concentrations. the duration, frequency, and concentration of p haemolytica shed from the inoculated nostrils ... | 1989 | 2551200 |
| histological changes in lungs of calves exposed to an aerosol of pasteurella haemolytica. | an experiment was designed to study the interaction of pasteurella haemolytica with an attenuated bovine herpesvirus 1 in calves. low titre of the virus culture used for aerosol exposure failed to produce measurable interaction. however, the experiment provided the first opportunity to study the light-microscopic changes in lungs of calves (n = 3) to a low-dose exposure (5-min aerosol) of p. haemolytica a1 from a fresh 5-h log-phase culture. the histopathological study was confined to tissue exp ... | 1989 | 2551938 |
| spatial and temporal distribution of bovine herpesvirus 1 transcripts. | northern (rna) blot analysis was used to determine the spatial and temporal distribution of bovine herpesvirus 1 (bhv-1) transcripts. total rna was isolated from madin-darby bovine kidney cells which had been infected with bhv-1.2b strain k22 or bhv-1.1 strain jura in the presence or absence of metabolic inhibitors. cloned restriction fragments representing the entire genome of strain k22 were labeled with 32p and hybridized to immobilized rna. a total of 54 bhv-1 transcripts were found, ranging ... | 1989 | 2552160 |
| [cell-mediated immunity in calves immunized against or infected with the bovine rhinotracheitis virus]. | simple and expeditious methods--leucocyte adherence inhibition lai and leucocyte migration inhibition lmi--are described in the present paper; these methods enable to investigate cell-mediated immunity of animals. the two tests, along with serological and virological methods, were used to study changes in the immunity of calves after experimental infection by ibrv and after immunization by inactivated oil vaccine against ibrv. the results have indicated that the changes in cell-mediated immunity ... | 1989 | 2552637 |
| two different strains of an alphaherpesvirus can establish latency in the same tissue of the host animal: evidence from bovine herpesvirus 1. | we inoculated plaque-purified bovine herpesvirus type 1 (bhv 1), strain k22, subtype bhv 1.2b, intravenously into susceptible cattle. five months later, we reactivated latent virus with dexamethasone and then super-infected the same cattle intranasally and intravaginally with a different plaque-purified bhv 1, strain cooper, subtype bhv 1.1. after a second dexamethasone treatment four months later, reactivated viruses were isolated and examined with restriction endonucleases. we showed that both ... | 1989 | 2552959 |
| negative enrichment of bovine t lymphocytes with monoclonal antibodies and magnetic microspheres. | a highly enriched population of bovine t lymphocytes was produced from peripheral blood leukocytes following the depletion of monoclonal antibody-labelled b lymphocytes and monocytes with magnetic microspheres. this negative-enrichment protocol was simple, rapid, and specific. also, it had a high recovery efficiency and was consistently reproducible. the enriched t lymphocytes proliferated in response to recombinant bovine interleukin 2 and, following the addition of monocytes, to concanavalin a ... | 1989 | 2554562 |
| nucleotide sequence of bovine herpesvirus type 1 glycoprotein giii, a structural model for giii as a new member of the immunoglobulin superfamily, and implications for the homologous glycoproteins of other herpesviruses. | the gene encoding bovine herpesvirus type 1 (bhv-1) glycoprotein giii was mapped, cloned, and sequenced. the gene is situated between map units 0.122 and 0.135 and encodes a predicted protein of 521 amino acids. the identity of the sequenced gene has been verified previously by expression of immunologically authentic recombinant bhv-1 giii (fitzpatrick et al. (1988) j. virol. 62, 4239-4248). comparison of the bhv-1 giii amino acid sequence with the homologous glycoproteins of other alphaherpesvi ... | 1989 | 2554578 |
| a comparison of polypeptides and restriction endonuclease sites of bhv-1 isolates and identification of ipv virus in japan. | bovine herpesvirus 1 (bhv-1) isolates from respiratory tract and from vagina of bovine in japan were analyzed by sds-polyacrylamide gel electrophoresis (sds-page) and the dna restriction endonuclease cleavage pattern, and compared with european bhv-1 strains. both protein profile and dna cleavaged pattern of bhv-1 isolates from respiratory tract were the same as those of european infectious bovine rhinotracheitis (ibr) virus, whereas the protein profile and dna cleavage patterns of one isolate ( ... | 1989 | 2557474 |
| a microplate immunofluorescence assay for the detection of monoclonal antibodies against viral antigens in cell culture. | a microplate immunofluorescence assay for the screening of culture fluids from large numbers of hybridoma clones has been developed. this method enables the simultaneous screening of a small volume of culture fluid from initial hybridoma clones on several different viruses or several strains of the same virus, enabling the early isolation and stabilization of the clones with the desired specificity. | 1989 | 2558137 |
| efficacy of a live pasteurella multocida vaccine for the prevention of experimentally induced bovine pneumonic pasteurellosis. | seventeen holstein-friesian calves weighing an average of 139.8 +/- 13.5 (mean +/- standard deviation) kg were used in a study to determine the efficacy of a live vaccine containing of pasteurella multocida a:3 and pasteurella haemolytica a:1. eleven calves received the vaccine by intramuscular injection in the right shoulder, whereas six calves received vaccine diluent and served as non-vaccinated controls. fourteen days following vaccination (day 15) all calves were inoculated deep intranasall ... | 1989 | 2558441 |
| changes in the restriction endonuclease patterns of four modified-live infectious bovine rhinotracheitis virus (ibrv) vaccines after one passage in host animal. | four genomically different bovine herpes virus 1 (bhv 1) vaccines were used to inoculate bhv 1-negative heifers. restriction endonuclease analysis (rea) of virus isolates from animals during acute infection, after reactivation of virus from latency, and after reactivation followed by superinfection, showed that changes occurred in the bhv 1 genome after one passage in the host animal. furthermore, the changes varied according to the tissue from which virus was isolated. southern probe analyses i ... | 1989 | 2558453 |
| bovine recombinant interleukin-2 augments immunity and resistance to bovine herpesvirus infection. | the in vivo administration of bovine recombinant interleukin-2 (ril-2) was evaluated in calves vaccinated and then challenged with bovine herpesvirus-1 (bhv-1). in experiment 1, 24 calves were allotted to four groups: control; bovine ril-2; bhv-1 vaccine (modified-live); and bovine ril-2 + bhv-1 vaccine. serum neutralizing antibody titers to bhv-1 were increased sixfold, and virus shedding was fourfold less in calves vaccinated and treated with ril-2 (25 micrograms/kg, intramuscularly) when comp ... | 1989 | 2559538 |
| [the detection of bovine herpesvirus type 1 (bhv 1) using an intradermal test. i. field studies]. | an intradermal test (delayed hypersensitivity test) for the diagnosis of bhv1 infection was evaluated in 791 cattle of 16 dairy farms. the skin reactions were compared with the results of serological examinations using a commercial bhv1 elisa kit (trachitest). as antigen concentrated, purified and inactivated bhv1 was used. the skin reaction (increase of the skin fold thickness) was used for the interpretation of test results. the best results were obtained with the control of the skin reaction ... | 1989 | 2559561 |
| [research on antibodies against bhv-1, bhv-2, bhv-4, bvd-md virus, bovine adenovirus a and b, rotavirus and coronavirus in cattle in western zaire: complementary results]. | two-hundred bovine sera from western zaire were screened for antibodies to 8 viruses: bhv-1, bhv-2, bhv-4, bvd-md virus, bovine adenovirus a and b, bovine rotavirus and bovine coronavirus. positive sera were found to all these viruses. for animals whose origin was undoubted, the main features were the high prevalence of infections by rotavirus and bhv-4 and the low prevalence of infections by coronavirus and bvd-md virus. | 1989 | 2560579 |
| dna restriction enzyme analysis of a bovine herpesvirus 1 strain isolated from encephalitis in hungary. | the dna of a bovine herpesvirus 1 (bhv-1) strain isolated from calf encephalitis in hungary was analysed with restriction enzymes. the cleavage pattern of the encephalitis strain na/67 differed from those of all the other hungarian bhv-1 isolates investigated so far. the ecori and hindiii cleavage patterns of virus strain na/67 were found to be similar to the patterns of two other encephalitis strains (n569 and a663 from australia and argentina, respectively) characterized earlier. strain na/67 ... | 1989 | 2561660 |
| molecular cloning of dna from a bovine herpesvirus 1 strain isolated in hungary. | molecular cloning of the hindiii fragments of bovine herpesvirus 1 (bhv-1) strain hb144, isolated from infectious bovine rhinotracheitis (ibr) in hungary, and of an infectious pustular vulvovaginitis (ipv) reference strain (k22) is reported. so far 52% of the ibr viral genome and 28% of the ipv viral genome have been cloned. the analysis of differences between the strains is currently in progress. | 1989 | 2561661 |
| the conserved open reading frame overlapping the thymidine kinase gene of different herpesviruses exists also in bovine herpesvirus 1. | similar open reading frames (orf) overlapping the thymidine kinase (tk) gene on the opposite strand or being close to it have been identified in 8 herpesviruses (jacobson et al., 1989). study of the dna sequence of bhv-1 tk gene published by mittal and field (1989) and kit and kit (1986) revealed the existence of a similar orf in this virus genome, too. | 1989 | 2561662 |
| demonstration of infectious bovine rhinotracheitis virus antigen in paraffin sections. | nine pregnant heifers were inoculated intravenously with infectious bovine rhinotracheitis virus (ibrv) in the sixth month of pregnancy. tissues were collected from the fetus of a heifer killed 13 days postinoculation (pi), from fetuses of 6 heifers that aborted 16-27 days pi, and from mummified fetuses of 2 heifers that aborted 53 and 85 days pi, respectively. control tissues were obtained from the fetus of a non-inoculated heifer that was killed in the seventh month of gestation. tissues were ... | 1989 | 2562191 |
| detection of bovine herpesvirus-1-specific igm using a capture enzyme immune assay with isotype-specific monoclonal antibodies. | the detection of virus-specific immunoglobulin m (igm) antibodies in acute-phase serum samples offers the possibility of making an accurate and rapid serologic diagnosis. we have developed a solid-phase capture assay that uses murine monoclonal antibodies specific for bovine igm to separate the whole igm fraction of a bovine serum sample. the igm specific for bovine herpesvirus-1 (bhv-1) is then detected by the addition of viral antigen, which in turn is detected by bhv-1-specific monoclonal ant ... | 1989 | 2562193 |
| use of in situ hybridization with a biotinylated probe for the detection of bovine herpesvirus-1 in aborted fetal tissue. | forty-five cases of bovine abortion were examined using in situ hybridization (ish) with a biotinylated dna probe specific for bovine herpesvirus-1 (bhv-1). of the 45 cases, 16 were diagnosed as due to bhv-1, 15 were determined to be due to other causes, and 14 were of undetermined etiology. direct comparisons between ish and an immunoperoxidase (ip) test specific for bhv-1 were performed on formalin-fixed, paraffin-embedded tissue sections of lung, liver, kidney, spleen, thymus, and placenta; f ... | 1989 | 2562195 |
| rapid fluorescence detection of in situ hybridization with biotinylated bovine herpesvirus-1 dna probes. | the use of biotinylated dna hybridization probes for clinical detection of bovine herpesvirus-1 was investigated. biotinylated dna hybridization probes were prepared from bovine herpesvirus-1 dna purified from infected cell cultures. the viral dna was nick translated in the presence of biotin-dutp with dna polymerase to incorporate biotin into the newly synthesized strand. the probe was tested for specificity in in situ hybridization assays with bovine herpesvirus-1 dna. hybridization was detect ... | 1989 | 2562208 |
| use of an immunoperoxidase test for the detection of bovine herpesvirus-1 in aborted fetal tissue. | an indirect immunoperoxidase (ip) procedure using a specific monoclonal antibody and an avidin-biotin-peroxidase complex was developed and applied to detect virus antigen in formalin-fixed, paraffin-embedded tissue sections. this ip procedure was compared with currently used diagnostic tests for detection of virus-induced abortions caused by bovine herpesvirus-1 (bhv-1). the ip procedure was applied to detect bhv-1 antigen in sections of liver and lung from 87 aborted fetuses. sixteen of these c ... | 1989 | 2562209 |
| effect of viral infection of fc receptors and immunologically nonspecific receptors of blood polymorphonuclear leukocytes: an in vitro model. | i describe the effect on phagocytic activity of the migration of bovine blood polymorphonuclear leukocytes (pmnl) through collagen and a monolayer of pk-15 cells in vitro infected with rotaviruses (strain osu, rfc-17, sa-11), a picornavirus (strain svdv), bovine herpesvirus (bhv-1) and equine herpesvirus (ehv-1). the pmnl were examined before and after their migration as follows: (1) for ea rosette formation with sensitized sheep erythrocytes, (2) for phagocytic activity mediated through fc rece ... | 1989 | 2569805 |
| viral-bacterial pneumonia in calves: effect of bovine herpesvirus-1 on immunologic functions. | the temporal relation between a lung infection with bovine herpesvirus-1, suppression of some immune functions, and susceptibility to secondary bacterial infection resulting in fibrinous pneumonia prompted a study to determine the mechanism(s) involved in the apparent immunosuppression. in six independent experiments employing from five to 40 calves, we studied the immunologic parameters of migration of and superoxide anion production by polymorphonuclear neutrophils, lectin-induced lymphocyte p ... | 1985 | 2580916 |
| the pathology of concurrent bovine viral diarrhoea and infectious bovine rhinotracheitis virus infection in newborn calves. | concurrent bovine viral diarrhoea (bvd) and systemic infectious bovine rhinotracheitis (ibr) are reported from two neonatal (11 and 15 days old) calves. the diseases occurred sporadically in a large-scale herd which may have been due to the calves' heterogeneous immunobiological status. gross pathological and histopathological examinations revealed focal interstitial pneumonia with acidophilic intranuclear inclusions in the alveolar epithelial cells and necrotic foci in the liver with a few intr ... | 1989 | 2627007 |
| the evolution of vaccines for bovine pneumonic pasteurellosis. | since the early 1900s bovine pneumonic pasteurellosis has been recognised as a major economic problem to european and north american cattle industries. initial attempts to prevent the disease were complicated by incomplete knowledge of the causative organisms. despite some early reports of vaccine-induced protection against disease, initial vaccines were of questionable protective value. from the late 1950s to the 1970s pasteurella haemolytica and p multocida bacterins were the primary type of v ... | 1989 | 2672200 |
| 2',5' oligoadenylate synthetase activity in bovine peripheral blood mononuclear cells following bovine herpesvirus type-1-induced respiratory disease: a prognostic indicator? | following aerosol-challenge of nonimmune calves with bovine herpesvirus type-1 (bhv-1) the levels of 2',5' oligoadenylate (2-5a) synthetase in peripheral blood mononuclear cells (pbml) increased significantly to peak 4-5 days after bhv-1 infection. this corresponded temporally to the period of highest susceptibility to secondary bacterial infection. ten days post virus infection, the enzyme levels had returned to baseline values. aerosol challenge with bacteria (pasteurella haemolytica) did not ... | 1989 | 2715670 |
| the frequency, distribution and effects of antibodies, to seven putative respiratory pathogens, on respiratory disease and weight gain in feedlot calves in ontario. | during 1983-85, 279 calves requiring treatment for bovine respiratory disease and 290 comparison (control) animals from 15 different groups of feedlot calves were bled on arrival and again at 28 days postarrival. their sera were then analyzed for antibodies to seven putative respiratory pathogens. on arrival, the prevalences of indirect agglutination titers to pasteurella haemolytica, p. haemolytica cytotoxin, mycoplasma bovis and m. dispar were greater than 50%, the prevalence of titers to bovi ... | 1989 | 2766158 |
| isolation of infectious bovine rhinotracheitis virus from a latently infected feral pig. | infectious bovine rhinotracheitis (ibr) virus was isolated from the trigeminal ganglion of a feral pig after dexamethasone treatment. three pigs inoculated intranasally with the ibr virus did not respond clinically or serologically. the virus was re-isolated from tonsillar swabs from two animals on post-infection day (pid) 3. | 1987 | 2821673 |
| the inhibition of bovine herpesvirus-1 by methyl 2-pyridyl ketone thiosemicarbazone and its effects on bovine cells. | methyl 2-pyridyl ketone thiosemicarbazone (mpkt) was found to inhibit bovine herpesvirus-1 (bhv-1) at an ed50 concentration of approximately 5-10 microm. several virus strains were shown to have similar sensitivity to the drug and serial passage of virus in the presence of mpkt failed to yield resistant progeny. there was evidence for toxic effects on cells at drug concentrations similar to those required to inhibit virus and passage of cells in low concentrations of mpkt gave results suggesting ... | 1987 | 2821894 |
| vaccination of rabbits with a bovine herpesvirus type 1 subunit vaccine: adjuvant effect of iscoms. | bovine herpesvirus type 1 has two major immunogenic surface glycoproteins: a 90 kda haemagglutinin and the 130 (74 + 54) kda glycoprotein. these proteins were purified by rate zonal sucrose density gradient centrifugation after extraction with nonionic detergent triton x-100. for the preparation of iscoms, the glycoproteins were further adsorbed during a second rate zonal centrifugation, to micelles of quil a glycoside, already added to the gradient. haemagglutinating peaks were collected and us ... | 1987 | 2823496 |
| specificity of the indirect enzyme-linked immunosorbent assay for detection of pseudorabies virus antibodies in pigs exposed to bovine herpesvirus-1. | six 5-week-old pigs were inoculated intranasally (in) with 10(7.6) tcid50 of bovine herpesvirus-1 (bhv-1). three of the pigs also were inoculated iv with a similar dose of bhv-1. clinical responses were not observed in these 6 pigs before oronasal challenge exposure with 10(7.8) tcid50 of virulent pseudorabies virus (prv) at postinoculation day 42. two pigs inoculated in with bhv-1 and challenge exposed with prv remained healthy, whereas the remaining 4 pigs developed severe clinical signs of ps ... | 1987 | 2823651 |
| mapping bovine herpesvirus type 1 latency-related rna in trigeminal ganglia of latently infected rabbits. | here we have used the bovine herpesvirus type 1 (bhv-1) rabbit model together with in situ nucleic acid hybridization to identify and map viral rna present in latently infected neurons. radioactively labeled cloned hindiii fragments representing most of the bhv-1 genome (cooper strain) were individually hybridized to sections of trigeminal ganglia taken from rabbits during acute and latent stages of infection. whereas all viral genomic fragments hybridized to lytically infected tissue culture ce ... | 1987 | 2824817 |
| map location of the thymidine kinase gene of bovine herpesvirus 1. | bovine herpesvirus 1 has been reported to contain a thymidine kinase (tk) gene which is nonessential for virus replication. we have isolated a thymidine kinase-negative mutant of the virus and localized the mutation by marker rescue experiments to a 1.1-kilobase bglii-sali fragment which maps at 0.47 to 0.48 on the bovine herpesvirus 1 genomic map. a thymidine kinase-negative bovine cell line isolated in our laboratory was used in these studies. | 1987 | 2824831 |
| bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination. | the 87,000-dalton glycoprotein (gp87) of bovine herpesvirus type 1 (bhv-1) was found to selectively attach to susceptible cells. the attachment of gp87 to the cells was markedly decreased by the prior adsorption of intact virions. anti-gp87 (site ia) monoclonal antibody, which inhibited bhv-1 adsorption to the cells and neutralized the virus without complement [okazaki et al., virology 250: 260-264], was effective in inhibiting the adsorption of gp87. only the same antibody was able to inhibit t ... | 1987 | 2827610 |
| rapid, sensitive, competitive serologic enzyme-linked immunosorbent assay for detecting serum antibodies to bovine herpesvirus type 1. | an enzyme-linked immunosorbent assay (elisa) for the detection of cattle antibodies to bovine herpesvirus type 1 was developed on the basis of competition between serum antibody and a virus-neutralizing mouse monoclonal antibody. the assay showed improved sensitivity over the virus neutralization (vn) test and over an enhanced vn test in which incubation of antibody-virus mixtures was carried out for 24 h. with the elisa, antibodies in sera from experimentally infected cattle were detected earli ... | 1987 | 2828419 |
| effect of dietary selenium on the primary and secondary immune response in calves challenged with infectious bovine rhinotracheitis virus. | the present study was conducted to determine if a selenium deficiency affects the primary and secondary humoral immune response of calves challenged with infectious bovine rhinotracheitis virus (ibrv) and to evaluate changes in glutathione peroxidase (gsh-px) and creatine phosphokinase activities associated with selenium deficiency and viral stress. treatments included 1) +se (0.2 mg/kg diet) and 2) -se (0.03 mg/kg diet). calves were adapted to their assigned diets during an 84-d depletion perio ... | 1988 | 2828581 |
| pasteurella haemolytica serotype 1 colonization of the nasal passages of virus-infected calves. | nasal passages of calves with a virus-induced respiratory tract disease became colonized by pasteurella haemolytica serotype 1 after they were inoculated intranasally with p haemolytica. inoculation with infectious bovine rhinotracheitis virus caused a more severe clinical illness and resulted in a greater degree of colonization with p haemolytica than developed after inoculation with parainfluenza-3 virus. nasal passages of parainfluenza-3 virus-inoculated calves were colonized to a greater deg ... | 1987 | 2829666 |
| genomic localization and sequence analysis of the putative bovine herpesvirus-1 dna polymerase gene. | the bovine herpesvirus-1 (bhv-1) genome was analysed by southern blot hybridization using the herpes simplex virus type 1 (hsv-1) dna polymerase gene as a probe. a 2.5 kilobase region which hybridized specifically to the hsv-1 dna polymerase gene was identified within the hind iii g fragment at approximate map units 0.334-0.352. in order to provide further evidence that this is the location of the bhv-1 dna polymerase gene, the 2.5 kilobase region was cloned and part of it sequenced. an uninterr ... | 1988 | 2829796 |
| [cellular and humoral immune responses in calves after experimental infection with bovine herpesvirus 1]. | studied were some of the humoral and cellular factors of immunity with calves experimentally infected with herpes virus 1. the clinical signs manifested by the infected calves were followed up for 10 to 12 days. the shedding of the virus through the nasal mucosa was demonstrated up to the eighth day, the peak values reached being between the 3rd and 5th day of infection. demonstrated was blastic transformation in vitro with a phytohemagglutinin of the lymphocytes obtained from the peripheral blo ... | 1987 | 2830708 |
| specificity of cleavage in replicative-form dna of bovine herpesvirus 1. | the linear double-stranded dna genome of herpesvirus as it is present in infectious virions needs to be circularized after infection of host cells and before dna replication. replicative-form genomes have to be cleaved into linear unit-length molecules during virion maturation and are most probably the substrate for inversion of the short segment relative to the long segment of the bovine herpesvirus 1 (bhv-1) genome. those regions of the bhv-1 genome which are functionally involved in these pro ... | 1988 | 2831397 |
| serological investigations for some bacterial and viral pathogens in fallow deer (cervus dama) and wild boar (sus scrofa) of the san rossore preserve, tuscany, italy. | sera of 43 fallow deer (cervus dama) of the san rossore preserve (tuscany, italy) were examined for antibodies against eight pathogens; one proved positive for brucella sp., 21 for listeria monocytogenes, 34 for chlamydia psittaci, three for coxiella burnetii, one for infectious bovine rhinotracheitis virus, 11 for parainfluenza-3 virus, 25 for bovine viral diarrhea virus and six for bovine respiratory syncytial virus. no age and sex difference in the positivity rates and titers was evidenced, w ... | 1988 | 2832622 |
| occurrence of bovine herpesvirus-1 dna in nucleosomes and chromatin of bovine herpesvirus-1-infected cells: identification of a virion-associated protein in chromatin of infected cells. | during virus replication a fraction of the intranuclear dna of bovine herpesvirus-1 (bhv-1) was present in the nucleosomal structure of infected eukaryotic cells, and virion proteins were associated with the chromatin of virus infected cells. synthesis of bhv-1 dna in bovine embryonic lung (bel) cells was found to begin four to six hours post-infection (p.i.) and to continue until at least 24 hours p.i. chromatin isolated from infected cell nuclei at ten hours p.i. contained both bhv-1 viral and ... | 1988 | 2835948 |
| serological survey of herpesvirus infections in wild ruminants of france and belgium. | the presence of antibodies against bovine herpesvirus 1 (bhv-1), bovid herpesvirus 6 (bhv-6), herpesvirus of cervidae type 1 (hvc-1), reindeer herpesvirus, bovine herpesvirus 2 (bhv-2) and bovid herpesvirus 4 (bhv-4) was investigated in wild ruminants of france and belgium between 1981 and 1986. there were no animals serologically positive for bhv-4. antibodies against bhv-2 were demonstrated in roe deer (cervus capreolus) (less than 1%) and chamois (rupicapra rupicapra) (1%) in france. animals ... | 1988 | 2836635 |
| evaluation of an antigen-capture elisa for the detection of bovine herpesvirus type 1 shedding from feedlot cattle. | a total of 457 nasal swab specimens from cases of respiratory disease in 2 feed lots were evaluated for the detection of bovine herpesvirus type 1 (bhv-1) by elisa. thirty-three were found to be positive for bhv-1 by the recovery of infectious virus and 21 of these were positive by elisa, yielding a sensitivity of 64%. fifteen other virus isolations were made and included bovine viral diarrhea viruses, rhinoviruses and parainfluenza type 3 viruses; none of these cases were positive with the bhv- ... | 1988 | 2836986 |
| identification of major antigenic proteins of bovine herpesvirus 1 and their correlation with virus neutralizing activity. | the polypeptides of an australian isolate of bovine herpesvirus 1 were analysed by polyacrylamide gel electrophoresis, and western blotting was used to identify those polypeptides which reacted most strongly with sera from infected animals. approximately 20 polypeptides ranging in molecular weight from 11,000 to 240,000 daltons (11-240k) were identified by 35s-methionine labelling of virus and approximately half of these classed as glycoproteins using 14c-mannose and 3h-glucosamine incorporation ... | 1988 | 2836987 |
| prevalence of antibodies to seven viruses in a flock of ewes in minnesota. | blood samples were collected from a flock of healthy ewes at a university of minnesota research station. sera from these blood samples were tested for antibodies against 7 viruses, using 3 tests (eg, virus-neutralization test for bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, bovine adenovirus type 3, and bovine respiratory syncytial virus; hemagglutination inhibition test for parainfluenza virus type 3; and agar-gel immunodiffusion test for lentivirus of ovine progressive ... | 1988 | 2837112 |
| protection of newborn calves against fatal multisystemic infectious bovine rhinotracheitis by feeding colostrum from vaccinated cows. | to determine whether consumption of colostrum with high levels of serum neutralizing antibody to bovine herpesvirus 1 would protect neonatal calves from the frequently fatal multisystemic form of infectious bovine rhinotracheitis, holstein calves were fed for 48 h after birth with either pooled colostrum from seropositive vaccinated cows or colostrum from seronegative unvaccinated cows. the serum neutralizing antibody achieved in the former calves was between 64 and 256 and the titer in the latt ... | 1987 | 2839276 |
| vaccination to protect calves against infectious bovine rhinotracheitis. | a commercially available inactivated vaccine against infectious bovine rhinotracheitis (bhv1) was tested to assess its ability to immunise young seronegative calves and protect them against challenge with a virulent strain of bhv1. calves showed seroconversion after one or two doses of vaccine. a two-dose and three-dose vaccination regimen each afforded calves significant protection against challenge as judged by the development of clinical symptoms. vaccinated calves were on average 7 to 10 kg ... | 1988 | 2839926 |
| effects of infection with parainfluenza-3 virus and infectious bovine rhinotracheitis virus on neutrophil functions in calves. | calves were challenge exposed in separate experiments with parainfluenza-3 (pi-3) virus or infectious bovine rhinotracheitis (ibr) virus. blood neutrophils were assayed for functional activity every other day for at least 3 weeks by random migration, staphylococcus aureus ingestion, antibody-dependent cell-mediated cytotoxicity, cytochrome-c reduction, iodination, and native chemiluminescence. exposure to pi-3 virus resulted in a brief febrile response and no other clinical signs. alterations in ... | 1988 | 2840008 |
| [surveillance investigations of brucellosis-, leukosis- and bhv-free cattle herds. elisa-based bulk milk studies compared to single animal sample studies with traditional test systems. safety and cost]. | | 1988 | 2840257 |
| characterization of bovine herpesviruses isolated from six sheep and four goats by restriction endonuclease analysis and radioimmunoprecipitation. | viral dna from 10 herpesviruses isolated from 6 sheep and 4 goats were examined by restriction endonuclease analysis with respect to their relatedness to one another; to bovine herpesvirus type 6 (bhv-6), also known as caprine herpesvirus; and to 2 strains of bovine herpesvirus type 1 (bhv-1), known as infectious bovine rhinotracheitis virus (ibrv) and infectious pustular vulvovaginitis virus (ipvv). viral proteins from the isolates were examined by radioimmunoprecipitation with anti-bhv-1/ibrv ... | 1988 | 2840840 |
| comparison of the bovine herpesvirus 1 gi gene and the herpes simplex virus type 1 gb gene. | in a previous report, we localized the gene for a 130-kilodalton envelope glycoprotein (gi) of bovine herpesvirus 1 (bhv-1) to a 3.6-kilobase hpai-kpni restriction endonuclease fragment from the long unique region of the bhv-1 genome (map position 0.405 to 0.432) and showed that a herpes simplex virus 1 (hsv-1) glycoprotein b (gb) probe uniquely hybridized to this bhv-1 restriction fragment. here we present the complete nucleotide sequence of the bhv-1 gi gene and the predicted 932-amino-acid se ... | 1988 | 2841484 |
| associations between viral infection and respiratory disease in young beef bulls. | blood samples were taken from bull calves at two meat and livestock commission performance testing centres, just after weaning at six months of age and at six weekly intervals until the end of the performance test seven months later. sera were assayed by specific elisas for antibodies to bovine herpes virus 1 (bhv1), respiratory syncytial virus, parainfluenza 3 (pi3) and adenoviruses a and b. seroconversions between each sampling were related to the occurrence of clinical respiratory disease usi ... | 1988 | 2842921 |
| an attempt to establish a herd serologically negative for infectious bovine rhinotracheitis. | to improve his position as an exporter of pedigree animals, an owner wished to establish his herd as serologically negative for infectious bovine rhinotracheitis (ibr). after a test on all animals older than six months the herd was split into seronegative and seropositive groups. the seronegative group was tested four times during the first year, twice during the second year and given a final test 12 months later; any positive animals were removed. the results indicated that in the right circums ... | 1988 | 2842923 |
| expression of bovine herpesvirus 1 glycoproteins gi and giii in transfected murine cells. | genes encoding two of the major glycoproteins of bovine herpesvirus 1 (bhv-1), gi and giii, were cloned into the eucaryotic expression vectors prsvcat and psv2neo and transfected into murine lmtk- cells, and cloned cell lines were established. the relative amounts of gi or giii expressed from the two vectors were similar. expression of gi was cell associated and localized predominantly in the perinuclear region, but nuclear and plasma membrane staining was also observed. expression of gi was add ... | 1988 | 2845135 |
| antibodies to bovine bacterial and viral pathogens in pronghorns in alberta, 1983. | sera from 210 pronghorns (antilocapra americana) ranging in southeastern alberta were tested for antibodies to disease agents present in indigenous cattle. no antibodies to brucella abortus, leptospira interrogans serovars pomona, hardjo, or grippotyphosa, or infectious bovine rhinotracheitis virus were found. antibodies at prevalences of 43.8% and 49.2% were detected to bovine virus diarrhea (bvd) and parainfluenza type 3 (pi-3) viruses, respectively. the much higher prevalence of bvd virus ant ... | 1986 | 2845155 |
| bovine herpesvirus 1: rapid diagnosis of infection by direct filter hybridization. | direct filter hybridization (dfh) was applied as a simple method of nucleic acid hybridization to diagnose bovine herpesvirus 1 (bhv-1) infection without previous purification of nucleic acids from the specimens. the dna of bhv-1 was cleaved with the restriction endonuclease pst i and randomly cloned into pkh47 plasmids. the clones were labelled with 32p or biotin and selected on uninfected and infected cells for the highest specific activity to detect bhv-1 infection. two clones, which detected ... | 1988 | 2845258 |
| sequence of a bovine herpesvirus type-1 glycoprotein gene that is homologous to the herpes simplex gene for the glycoprotein gb. | the 130-kda bovine herpesvirus-1 (bhv-1) glycoprotein gvp 6 was found to cross-react immunologically with the herpes simplex glycoprotein gb. antibodies in polyclonal serum against gb immunoprecipitated gvp 6 and its cleavage products from a lysate of bhv-1-infected cells. conversely, polyclonal serum against gvp 6 precipitated gb from hsv-1-infected cell lysates. sera against the other glycoproteins did not demonstrate cross-reactivity. a 3.6-kb kpnl-hpal fragment of bhv-1 dna that hybridized t ... | 1988 | 2845660 |
| propagation and quantitation of animal herpesviruses in eight cell culture systems. | a comparative study was carried out to determine the relative sensitivities of eight different cell culture systems to six different herpesviruses of animals. the cells used were: ofl (ovine fetal lung), ml (mink lung), fk (ferret kidney), ptk-2 (potoroo kidney), tek (turkey embryo kidney), ed (equine dermal), bt (bovine turbinate), and pk15 (porcine kidney). the viruses tested were: prv (pseudorabies) of swine, cphv (caprine herpesvirus), ibrv (infectious bovine rhinotracheitis virus), dn-599 s ... | 1988 | 2846231 |
| effects of a bovine herpesvirus-1 isolate on reproductive function in heifers: classification as a type-2 (infectious pustular vulvovaginitis) virus by restriction endonuclease analysis of viral dna. | a bovine herpesvirus-1 (bhv-1) isolate (fi) from an aborted fetus was used to infect 9 heifers at various stages of gestation. two heifers were inoculated iv on postbreeding day (pbd) 1, 7, or 14, and 3 heifers were inoculated in the sixth month of pregnancy. plasma progesterone assays were used to monitor corpus luteum function in heifers inoculated during early pregnancy. low progesterone values and infertility were seen in the 2 heifers inoculated on pbd 1. luteal function remained normal in ... | 1988 | 2847601 |
| immune production of interferon by cultured peripheral blood mononuclear cells from calves infected with bhv1 and pi3 viruses. | peripheral blood mononuclear cells (pbmc) from calves infected with bovine herpesvirus type 1 (bhv1) or parainfluenza 3 virus (pi3) were cultured in vitro in the presence of inactivated specific antigen presented on mdbk cells. in the presence of inactivated antigen, pbmc from both bhv1-infected and control calves produced interferon (ifn)-alpha in 24 hour cultures. altering the culture conditions did not result in the detection of immune-specific ifn produced by mononuclear cells from bhv1-infe ... | 1988 | 2848301 |
| serological comparisons of antigenically related herpesviruses in cattle, red deer and goats. | serological comparisons were made using related herpesviruses from cattle (bovid herpesvirus 1), red deer (herpesvirus of cervidae 1) and goats (bovid herpesvirus 6) by virus neutralization and enzyme-linked immunosorbent assays. the test samples comprised field sera from british cattle, red deer and goats and sera from experimentally infected or immunized animals. both the cervine and caprine viruses appeared to be more closely related to bovid herpesvirus 1 than they were to each other. cattle ... | 1988 | 2848356 |
| [acid-stable and acid-labile interferons inducible by the bovine infectious rhinotracheitis virus]. | attenuated and virulent strains of cattle infectious rhinotracheitis virus (irtv) induced in cattle leukocytes the production of both acid-stable and acid-labile interferon. the attenuated irtv strain induced the production of the acid-labile interferon in spleen and bone marrow cells of balb/c mice. the indication of the assignment of the acid-labile interferon to the leukocyte-type of interferon consists in the activity of bovine interferon in a heterologous culture of human diploid fibroblast ... | 1988 | 2848365 |