| construction of a new shuttle vector for lactobacillus. | to clone the malolactic enzyme gene from lactobacillus sp. 89, construction of a shuttle vector able to express itself in lactobacillus sp. 89 and escherichia coli was undertaken. the shuttle plasmid ple16 resulted from the union of pbr328 and of the plb10 plasmid extracted from lactobacillus bulgaricus 10. the bacterial transformation in lactobacillus sp. 89 was performed by electroporation, and the clones were selected on mrs medium with 30 micrograms.ml-1 chloramphenicol added. fifty percent ... | 1992 | 1581865 |
| [the sulfate-reducing capacity of bacteria in the genus pseudomonas]. | active sulphate-reducing microorganism which belongs to the genus pseudomonas has been distinguished and described. the culture is a facultative aerobe, optimum eh is -170-180 mv. pseudomonas sp. being cultivated under strictly anaerobic conditions sulphate-reduction proceeds more intensively than under aerobic conditions. this fact should be taken into account under treatment of industrial sewage. | 1992 | 1584088 |
| pathogenesis of widened ap-sp complex in cases of cerebellopontine angle tumors. | pathogenesis of widened ap-sp complexes often noted in electrocochleograms (ecochgs) in cases of cerebellopontine angle tumors remains unclear. as acoustic neuromas usually originate from the vestibular nerve, some changes in this nerve might be involved in producing the broadened waveform. effects of lidocaine on the ap-sp complex were investigated in guinea pigs by injecting this compound into the root exit zone of the vestibular nerve, where the cochlear efferent fibers run together. the ap-s ... | 1992 | 1584587 |
| purification and characterization of the ferredoxin-glutamate synthase from the unicellular cyanobacterium synechococcus sp. pcc 6301. | ferredoxin-glutamate synthase from the unicellular cyanobacterium synechococcus sp. pcc 6301 has been purified using, as main steps, ethanol fractionation in the presence of high ionic strength, ion-exchange chromatography and ferredoxin-sepharose affinity chromatography. the overall process yielded an homogeneous enzyme with a specific activity of 30 u/mg protein, after a purification of 2800-fold with a recovery of 43%. the molecular mass of the native protein was 156 kda, as calculated from i ... | 1992 | 1587284 |
| cloning and sequencing of pseudomonas genes determining sodium dodecyl sulfate biodegradation. | the nucleotide sequences of two genes involved in sodium dodecyl sulfate (sds) degradation, by pseudomonas, have been determined. one of these, sdsa, codes for an alkyl sulfatase (58,957 da) and has similarity (31.8% identity over a 201-amino acid stretch) to the n terminus of a predicted protein of unknown function from mycobacterium tuberculosis. the other gene, sdsb, codes for a positive activator protein (33,600 da) that has extensive similarity with the lysr family of helix-turn-helix dna-b ... | 1992 | 1587481 |
| patients at risk for inappropriate antibiotic treatment of bacteraemia. | in order to define patients at high risk for inappropriate antibiotic treatment of bacteraemia, we compared 682 bacteraemic patients, treated with an antibiotic drug to which the infecting micro-organism was susceptible, with 419 patients who were inappropriately treated. on a multivariate logistic regression analysis including only clinical variables, four factors were found to be both significantly and independently associated with inappropriate antibiotic treatment: hospital-acquired bacterae ... | 1992 | 1588261 |
| nucleotide sequence of the structural gene encoding a 2-haloalkanoic acid dehalogenase of pseudomonas putida strain aj1 and purification of the encoded protein. | the nucleotide sequence of a gene encoding an l-2-haloalkanoic acid halidohydrolase from pseudomonas putida strain aj1 was determined. the orf (hadl) codes for a polypeptide of 227 amino acids (mr 25,687) which has significant homology to two other l-2-haloalkanoic acid halidohydrolases of pseudomonas sp., dehci and dehcii; these show 38% and 51% amino acid identity respectively to hadl. all three enzymes produce products of an opposite optical configuration to that of the substrates. comparison ... | 1992 | 1588303 |
| identification, characterization and sequence analysis of the gene encoding phosphoenolpyruvate carboxylase in anabaena sp. pcc 7120. | the gene (ppc) encoding phosphoenolpyruvate carboxylase (pepcase) in the cyanobacterium anabaena sp. pcc 7120 has been isolated, characterized and its nucleotide sequence determined. heterologous hybridization using the synechococcus sp. pcc 7942 ppc gene as a probe of an anabaena genomic dna library identified an 8.2 kb hindiii dna fragment that contained a 3.08 kb open reading frame encoding the cyanobacterial pepcase. deletion analysis of the 8.2 kb dna fragment was used to determine sequence ... | 1992 | 1588304 |
| expression of the b subunit of escherichia coli heat-labile enterotoxin in a marine vibrio and in a mutant that is pleiotropically defective in the secretion of extracellular proteins. | a marine vibrio (designated vibrio sp. 60) that is related to vibrio anguillarum was used as a host for a plasmid that encodes the non-toxic b subunit (etxb) of escherichia coli heat-labile enterotoxin. expression of etxb in vibrio sp. 60 resulted in the efficient and selective secretion of the b subunit into the extracellular growth medium. this indicated that vibrio sp. 60, which does not normally produce cholera-like enterotoxins, nonetheless possesses a secretory machinery that permits these ... | 1992 | 1588306 |
| purification and characterization of tdp-d-glucose 4,6-dehydratase from anthracycline-producing streptomycetes. | tdp-d-glucose 4,6-dehydratase, which converts tdp-d-glucose to tdp-d-4-keto-6-deoxyglucose, was purified to near-homogeneity from the daunorubicin and baumycin-producing organism streptomyces sp. c5 (968-fold purification with a 41% recovery), and from the daunorubicin producer streptomyces peucetius atcc 29050 (1000-fold purification with a 37% recovery). the tdp-d-glucose 4,6-dehydratases from streptomyces sp. c5 and s. peucetius were determined by sds-page and hplc gel filtration to be homodi ... | 1992 | 1588310 |
| genome typing of southern african subgroup 1 geminiviruses. | the relatedness of subgroup 1 geminiviruses from a variety of naturally infected southern african graminaceous hosts was compared by dna cross-hybridization, restriction endonuclease mapping and partial sequencing. cross-hybridization divided the viruses into three groups: those closely related to maize streak virus (msvs), and separate groups comprising a panicum sp. virus (pansv) and two sugarcane viruses (ssvs). restriction mapping and comparisons, and phylogeny reconstructions from map data, ... | 1992 | 1588313 |
| effects of dietary fish oil on myocardial ischemic/reperfusion injury of wistar kyoto and stroke-prone spontaneously hypertensive rats. | the purpose of the present study was to determine whether 15 weeks of dietary fish oil supplementation to wistar kyoto (wky) and stroke-prone spontaneously hypertensive rats (sp-shr) would alter cardiac contractile performance. isolated perfused working hearts were used to determine the effects of varying left atrial filling pressures on cardiac pump output and pressure development. in addition, the ability of these hearts to recover cardiac output after a 60-minute period of low-flow ischemia f ... | 1992 | 1588834 |
| [microorganisms isolated from chronic suppurative otitis media and their antimicrobial sensitivities]. | in this study mycologic and bacteriologic cultures were made in 100 patients who had chronic suppurative otitis media. we observed 58.8% pure, 35.3% mixed growth (more than one bacteria or bacteria and fungus) in the cultures. in pure cultures, we found mostly proteus sp., s. aureus and pseudomonas sp. respectively. in 134 bacteriologic cultures most frequently isolated bacteria was s. aureus (36.6%). in sabouraud agar, we found mostly aspergillus sp. and candida sp. (28.6%). in antimicrobial se ... | 1992 | 1588848 |
| splanchnic and whole body l-[1-13c,15n]leucine kinetics in relation to enteral and parenteral amino acid supply. | the effect of the route of administration of a complete amino acid solution (0.24 g.kg-1.h-1) on leucine (leu) and alpha-ketoisocaproate (kic) metabolism in the splanchnic region (sp) was assessed in nine chronically catherized mongrel dogs receiving, for 6 h, amino acids by jugular vein (pn feeding). results were compared with those obtained previously [y. m. yu, d. a. wagner, e. e. tredget, j.a. waleszewski, j. f. burke, and v. r. young. am. j. physiol. 259 (endocrinol. metab. 22): e36-e51, 19 ... | 1992 | 1590378 |
| an allelic variant of the 6a gene for human surfactant protein a. | two human surfactant protein a (sp-a) cdna clones (1a and 6a) encoding human sp-a genes have previously been identified and characterized by our laboratory. the present study was undertaken to assess potential heterogeneity in the 6a locus among specimens from white adults and infants with respiratory distress syndrome (rds) as well as black adults. the entire and/or a portion of the published sp-a gene was amplified from different genomic dnas using the polymerase chain reaction (pcr), cloned, ... | 1992 | 1590407 |
| construction of insertion mutants of synechocystis sp. pcc 6803: evidence for an essential function of subunit iv of the cytochrome b6/f complex. | the gene encoding subunit iv of the cytochrome b6/f complex (petd) has been isolated from a genomic library of the unicellular cyanobacterium synechocystis sp. pcc 6803. the coding region consists of 480 nucleotides and can code for a polypeptide with a molecular weight of 17.5 kda. the deduced amino acid sequence shows high identity with the corresponding sequences of both the photoautotrophic prokaryote nostoc sp. pcc 7906 as well as of lower and higher photoautotrophic eukaryotes (e.g. chlore ... | 1992 | 1590707 |
| surfactant protein c is expressed in alveolar type ii cells but not in clara cells of rat lung. | the expression of the surfactant-associated proteins in bronchiolar cells remains to be defined. we used in situ hybridization to identify sites of message expression of the surfactant-associated proteins a, b, and c (sp-a, sp-b, and sp-c) in adult and fetal rat lung. the expression of these messages by in situ hybridization was also compared with the localization of sp-a by immunocytochemistry. the localization of sp-a was used to identify type ii cells and nonciliated bronchiolar epithelial (c ... | 1992 | 1591008 |
| the c-terminal domain of the pulmonary surfactant protein c precursor contains signals for intracellular targeting. | we have studied the intracellular transport of the pulmonary surfactant sp-c precursor in vitro and in vivo. in order to monitor the route of the sp-c precursor, we constructed various c-terminally truncated forms of sp-c, which were tagged with a sequence derived from the c-terminus of the human c-myc gene (aa 409-419). expression of these constructs under the control of the sv40 enhancer and the humt-ii promoter in stably transformed chinese hamster ovary (cho) cells revealed that the complete ... | 1992 | 1591009 |
| molecular and developmental studies of a sperm acrosome antigen recognized by hs-63 monoclonal antibody. | a conserved mouse sperm antigen (msa-63) recognized by a monoclonal antibody (hs-63) was isolated from mouse testes by single-step immunoaffinity chromatography. isolated msa-63 preparation was shown to be a group of proteins ranging from 24-84 kda and with isoelectric points (pis) ranging from 4.0-6.0 when analyzed by two-dimensional (2-d) gel electrophoresis. microsequencing techniques were employed to determine the relationships of various protein spots on 2-d gels. partial amino acid sequenc ... | 1992 | 1591350 |
| interactions of human sperm acrosomal protein sp-10 with the acrosomal membranes. | the interaction of the human acrosomal protein sp-10 with the acrosomal membranes was analyzed by the ability of triton x-114 (tx-114) and other agents to release sp-10 from the acrosome. treatment of human sperm with tx-114 revealed a pool of sp-10 that was released by tx-114 and a pool of sp-10 that was tx-114-resistant. tx-114-resistant sp-10 was associated with the equatorial segment and with tx-114-resistant portions of the acrosomal matrix and the inner acrosomal membrane. phase partitioni ... | 1992 | 1591355 |
| effects of atropine, pirenzepine, imipramine and phenothiazines on the mammalian neuromuscular junction. | 1. trifluoperazine (ec50 = 11.5 nm), chlorpromazine (13.8 microm), imipramine (15 microm), atropine (75.8 microm), and pirenzepine (316.2 microm), all produced neuromuscular facilitation and antagonized the blockade produced by oxotremorine (20 and 30 microm) in the rat isolated diaphragm. 2. these antagonists did not change the responses of curarized diaphragms to direct stimulation, or the twitch tension produced by retrograde injection of acetylcholine. 3. trifluoperazine (2.5 ng, intra-arter ... | 1992 | 1592221 |
| sch 42137, a novel antifungal antibiotic from an actinoplanes sp. fermentation, isolation, structure and biological properties. | a novel polycyclic xanthone, sch 42137, related to the albofungin family of compounds was isolated from culture broth. its structure was determined by detailed spectroscopic studies and comparison of circular dichroic studies to related compounds albofungin and simaomicin. sch 42137 exhibited mic values less than 0.125 micrograms/ml against yeasts and dermatophytes. details are presented herein. | 1992 | 1592676 |
| new polyenic antibiotics active against gram-positive and gram-negative bacteria. iv. structural elucidation of enacyloxin iia. | the chemical structure of a unique polyenic antibiotic enacyloxin iia (former name: fr. 2) produced by frateuria (formerly gluconobacter) sp. w-315 has been determined by extensive spectroscopic studies, in particular by nmr spectral analysis. it has a novel non-lactonic structure involving 3,4-dihydroxycyclohexanecarboxylic acid with a chlorine-containing polyenic and polyhydroxy acyl side chain attached as an ester to the 3-hydroxyl substituent of the acid. | 1992 | 1592680 |
| haloalkaliphilic maltotriose-forming alpha-amylase from the archaebacterium natronococcus sp. strain ah-36. | a haloalkaliphilic archaebacterium, natronococcus sp. strain ah-36, produced extracellularly a maltotriose-forming amylase. the amylase was purified to homogeneity by ethanol precipitation, hydroxylapatite chromatography, hydrophobic chromatography, and gel filtration. the molecular weight of the enzyme was estimated to be 74,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the amylase exhibited maximal activity at ph 8.7 and 55 degrees c in the presence of 2.5 m nacl. the activ ... | 1992 | 1592801 |
| anaerobic degradation of 2-aminobenzoic acid (anthranilic acid) via benzoyl-coenzyme a (coa) and cyclohex-1-enecarboxyl-coa in a denitrifying bacterium. | the enzymes catalyzing the initial reactions in the anaerobic degradation of 2-aminobenzoic acid (anthranilic acid) were studied with a denitrifying pseudomonas sp. anaerobically grown with 2-aminobenzoate and nitrate as the sole carbon and energy sources. cells grown on 2-aminobenzoate are simultaneously adapted to growth with benzoate, whereas cells grown on benzoate degrade 2-aminobenzoate several times less efficiently than benzoate. evidence for a new reductive pathway of aromatic metabolis ... | 1992 | 1592816 |
| transcriptional and posttranscriptional components of psba response to high light intensity in synechococcus sp. strain pcc 7942. | the psba genes, which encode the d1 protein of photosystem ii, constitute a multigene family in the cyanobacterium synechococcus sp. strain pcc 7942. levels of messages from the three psba genes change rapidly when cells are shifted from low-light to high-light conditions: the psbai message level drops, whereas psbaii and psbaiii message levels increase dramatically. we examined the potential contributions of transcriptional and posttranscriptional processes in these high-light responses by subj ... | 1992 | 1592828 |
| yeast flavohemoglobin is an ancient protein related to globins and a reductase family. | the hemoglobin of yeast is a two-domain protein with both heme and flavin prosthetic groups. the nucleotide sequences of the cdna and genomic dna encoding the protein from saccharomyces cerevisiae show that introns are absent and that both domains are homologous with a flavoheme protein from escherichia coli. the heme domains are also homologous with those of o2-binding heme proteins from several other distantly related bacteria, plants, and animals; all appear to be members of the same globin s ... | 1992 | 1594608 |
| copulatory behavior and reflexive penile erection in rats after section of the pudendal and genitofemoral nerves. | two experiments probed the roles of the pudendal and genitofemoral nerves in sexual behavior. male rats were tested for copulatory behavior and reflexive erections after transection of the sensory (sp) or motor (mp) branches of the pudendal nerves and, in experiment 2, section of the genitofemoral (gf) nerve alone or in combination with the sp nerves. damage to the gf nerve had no apparent effects. division of the sp nerve severely impaired the ability of males to achieve intromission, and hence ... | 1992 | 1594665 |
| in vitro conversion of surfactant subtypes is altered in alveolar surfactant isolated from injured lungs. | pulmonary alveolar surfactant can be separated into different subtypes on the basis of their buoyant densities. these subtypes have been characterized as ultraheavy and heavy forms, which are surface-active, and light forms, which are less surface active. the ratio of these subtypes was altered in an animal model of acute lung injury that contributed to the physiologic abnormalities. we used an in vitro method of surface-area cycling to compare conversion of heavy subtypes isolated from injured ... | 1992 | 1596011 |
| opiate activation suppresses the drinking, pressor and natriuretic responses induced by cholinergic stimulation of the medial septal area. | the present study investigates the participation and interaction between cholinergic and opiate receptors of the medial septal area (msa) in the regulation of na+, k+ and water excretion, drinking and blood pressure regulation. male holtzman rats were implanted with stainless steel cannulae opening into the msa. na+, k+ and water excretion, water intake and blood pressure were measured after injection of carbachol (cholinergic agonist), fk-33824 (an opiate agonist) + carbachol or naloxone (an op ... | 1992 | 1596737 |
| crystal structure of nad-dependent formate dehydrogenase. | the ternary complex of nad-dependent formate dehydrogenase (fdh) from the methylotrophic bacterium pseudomonas sp. 101 (enzyme-nad-azide) has been crystallised in the space group p2(1)2(1)2(1) with cell dimensions a = 11.60 nm, b = 11.33 nm, c = 6.34 nm. there is 1 dimeric molecule/asymmetric unit. an electron density map was calculated using phases from multiple isomorphous replacement at 0.30 nm resolution. four heavy atom derivatives were used. the map was improved by solvent flattening and m ... | 1992 | 1597184 |
| cestode parasites in potamotrygon motoro (natterer) (chondrichthyes: potamotrygonidae) from southwestern brazil, including rhinebothroides mclennanae n. sp. (tetraphyllidea: phyllobothriidae), and a revised host-parasite checklist for helminths inhabiting neotropical freshwater stingrays. | specimens of 5 species of cestodes were collected in 6 specimens of the freshwater stingray species potamotrygon motoro (natterer), collected in the vicinity of corumba, mato grosso do sul, brazil. acanthobothrium regoi, potamotrygonocestus orinocoensis, rhinebothroides venezuelensis, and rhinebothrium paratrygoni are reported from p. motoro and from southwestern brazil for the first time. rhinebothroides mclennanae n. sp. appears to be the sister species of rhinebothroides glandularis, the only ... | 1992 | 1597778 |
| neobenedenia pargueraensis n. sp. (monogenea: capsalidae) from the red hind, epinephelus guttatus, and comments about neobenedenia melleni. | neobenedenia pargueraensis n. sp. from epinephelus guttatus of puerto rico differs from all known species in possessing scoop-shaped accessory sclerites with pointed tips. it is most similar to neobenedenia melleni and neobenedenia longiprostata but differs from the former in having a fenestrate ovary and from the latter in that the ducts of the accessory glands are short, terminating just anterior to the vitelline reservoir rather than reaching the caudal end of the body. it differs from both s ... | 1992 | 1597779 |
| pre- and postnatal stimulation of pulmonary surfactant protein d by in vivo dexamethasone treatment of rats. | fetal (days 18 and 20 of gestation), neonatal (days 0, 2 and 4 of neonate) and adult rats were injected with dexamethasone (1 mg/kg) in vivo and 24 hours later the effect on the contents of surfactant protein d (sp-d) in the rat lungs were examined in comparison with surfactant protein a, disaturated phosphatidylcholine and phosphatidylglycerol. in vivo dexamethasone treatment resulted in significant increases of sp-d content as the other 3 components of surfactant in both fetuses and neonates, ... | 1992 | 1598064 |
| metabolic effect of pancreaticoduodenal transplantation in diabetic rats. | outbred wistar rats were randomly assigned to three experimental groups: gi, 10 nondiabetic control rats; gii, 10 alloxan-diabetic control rats; giii, 25 alloxan-diabetic rats that received pancreaticoduodenal transplantation (pdt) from normal donor wistar rats and were immunosuppressed with cyclosporin a. for 7 prior and 4, 7, 14, 21, and 30 days posttransplantation (during which the animals were housed in metabolic cages for periods of 24 hours) body weight, water and food intake, urine output ... | 1992 | 1598082 |
| purification and characterization of an extracellular muramidase of clostridium acetobutylicum atcc 824 that acts on non-n-acetylated peptidoglycan. | an extracellular enzyme showing lytic activity on non-n-acetylated peptidoglycan has been isolated from clostridium acetobutylicum atcc 824. the lytic enzyme was purified to homogeneity by anion-exchange chromatography and gel filtration, with a recovery of 24%. the enzyme was monomeric and had an estimated molecular weight of 41,000 and an isoelectric point of 3.8. it has been characterized as a muramidase whose 23-amino-acid n terminus displayed 39% homology with the n,o-diacetyl muramidase of ... | 1992 | 1599233 |
| degradation of hydrogen sulfide by xanthomonas sp. strain dy44 isolated from peat. | xanthomonas sp. strain dy44, capable of degrading h2s, was isolated from dimethyl disulfide-acclimated peat. this bacterium removed h2s either as a single gas or in the presence of the sulfur-containing compounds methanethiol, dimethyl sulfide, and dimethyl disulfide. the maximum specific h2s removal rate, obtained in the late stationary phase, was 3.92 mmol g of dry cells-1 h-1 (6.7 x 10(-16) mol cell-1 h-1) at ph 7 and 30 degrees c through a batch experiment in a basal mineral medium. since xa ... | 1992 | 1599238 |
| evaluation of aquatic sediment microcosms and their use in assessing possible effects of introduced microorganisms on ecosystem parameters. | in this paper we describe a sediment microcosm system consisting of 20 undisturbed, layered sediment cores with overlying site water which are incubated under identical conditions of temperature, light, stirring rate of overlying water, and water exchange rate. ecosystem parameters (nutrient level, photosynthetic potential, community structure of heterotrophic bacteria, thymidine incorporation rate, and oxygen microgradients) of the laboratory microcosms and the source ecosystem were compared an ... | 1992 | 1599244 |
| survival and function of a genetically engineered pseudomonad in aquatic sediment microcosms. | pseudomonas sp. strain b13 fr1(pfrc20p) is a genetically engineered microorganism (gem) which is able to degrade chloro- and methylaromatics through a constructed ortho cleavage pathway. the fate of the gem and its ability to degrade substituted aromatic compounds in two different aquatic sediments was investigated by using a microcosm system which consisted of intact layered sediment cores with an overlying water column. the gem survived in lake plussee and in rhine river sediments at densities ... | 1992 | 1599245 |
| evidence for an nih shift in oxidation of naphthalene by the marine cyanobacterium oscillatoria sp. strain jcm. | the marine cyanobacterium oscillatoria sp. strain jcm oxidized naphthalene predominantly to 1-naphthol. experiments with [1-2h]naphthalene and [2-2h]naphthalene indicated that 1-naphthol was formed with 68 and 74% retention of deuterium, respectively. no significant isotope effect was observed when the organism was incubated with a 1:1 mixture of naphthalene and [2h8]naphthalene. the results indicate that 1-naphthol is formed through a naphthalene 1,2-oxide intermediate, which rearranges spontan ... | 1992 | 1599253 |
| efficient fermentation of pinus sp. acid hydrolysates by an ethanologenic strain of escherichia coli. | process conditions for the acid hydrolysis of pine hemicellulose and cellulose have been described which provide a biocompatible sugar solution. by using an improved strain of recombinant escherichia coli, strain ko11, hydrolysates supplemented with yeast extract and tryptone nutrients were converted to ethanol with an efficiency of 85% to over 100% on the basis of monomer sugar content (approximately 72 g/liter) and with the production of 35 g of ethanol per liter in 48 h. in the process descri ... | 1992 | 1599258 |
| inhibition of mast cell secretion by oxidation products of natural polyamines. | mast cells secrete many biologically active compounds upon stimulation by immunoglobulin e (ige) and specific antigen (ag), anaphylatoxins, as well as a number of cationic compounds which include drugs, kinins and neuropeptides. the effects of the two naturally occurring polyamines, spermine (sp) and spermidine (spd), on mast cell secretion were studied because they have been implicated in the modulation of cellular processes, possibly through their cationic charge or the regulation of calcium i ... | 1992 | 1599509 |
| population genetic evidence for species a, b, c and d of the anopheles dirus complex in thailand and enzyme electromorphs for their identification. | mixtures of chromosomal forms a, b, c and d in natural populations of anopheles dirus peyton & harrison sensu lato in thailand show significant positive values of wright's fixation index for six enzyme-electromorph loci. the mean value of fis over all loci was found to be +0.28 (sd 0.02), with a range of +0.57 (odh) to +0.10 (idh-2). partitioning electromorph data for the chromosomal forms reduces the mean fis to 0.03 (sd 0.01), which suggests that positive assortative mating is a characteristic ... | 1992 | 1600224 |
| determinants of the domiciliary density of triatoma infestans, vector of chagas disease. | in two heavily infested rural villages of santiago del estero, argentina, where no indoor-spraying with residual insecticides had ever been carried out by official control services, we studied the influence of roof and wall structure, domestic use of insecticide, family size and the number of domestic dogs, on the domiciliary density of triatoma infestans (klug). bug density was significantly associated with (1) the interaction between insecticide use and type of roof, (2) the structure of indoo ... | 1992 | 1600232 |
| interaction of c1q receptor with lung surfactant protein a. | earlier we reported the purification of c1q receptor (c1qr) from u937 cells and human tonsil lymphocytes (malhotra, r. and sim, r. b., biochem. j. 1989. 218: 625) and showed that c1qr interacts with the ligands c1q, mannose-binding protein, conglutinin and lung surfactant protein a (sp-a) (malhotra, r., thiel, s., reid, k. b. m. and sim, r. b., j. exp. med. 1990. 172: 955). c1qr was characterized as an acidic glycoprotein, which, when solubilized, exists as a dimer of mr 115,000 under non-denatu ... | 1992 | 1601034 |
| structural organization of the gene encoding rat cystatin beta. | a genomic dna clone encompassing the gene (cy beta) encoding rat cystatin beta (cy beta) was isolated by screening with a rat cy beta cdna as a probe. the gene spans about 2.6 kb and comprises three exons. the first intron is located between lys22 and val23 and the second between lys56 and val57 in the deduced amino acid sequence of cy beta. the second exon contains the highly conserved qvvag sequence which, unlike the sequence of other cystatin family members, is not split by an intron. in the ... | 1992 | 1601307 |
| chlamydial-caused infectious keratoconjunctivitis in bighorn sheep of yellowstone national park. | an epizootic of infectious keratoconjunctivitis occurred in bighorn sheep (ovis canadensis) in yellowstone national park during the winter of 1981-82. the causative organism was identified as chlamydia sp. mortality related to the epizootic was approximately 60% of an estimated 500 bighorn sheep in the northern range population. the infection probably affected all sex and age classes, but field surveys of live animals and mortality suggested that mature rams died disproportionately. limited fiel ... | 1992 | 1602566 |
| spatial and seasonal distribution of potential vectors of hemorrhagic disease viruses to peninsular bighorn sheep in the santa rosa mountains of southern california. | blood-feeding midges (culicoides sp. and leptoconops sp.) were sampled in the santa rosa mountains, riverside county, california (usa), to determine which species might be involved in the transmission of bluetongue and epizootic hemorrhagic disease viruses to peninsular bighorn sheep (ovis canadensis cremnobates). host-seeking midges were sampled with co2-baited suction traps over a period of 30 mo. nineteen species of culicoides and seven of leptoconops were collected. five of the culicoides sp ... | 1992 | 1602570 |
| chlamydiosis in a red-tailed hawk (buteo jamaicensis). | a red-tailed hawk (buteo jamaicensis) with signs of respiratory distress and diarrhea was captured in the manchac wildlife management area, louisiana (usa) and died the following day. at necropsy, the carcass was emaciated and there were splenomegaly, and fibrinous pericarditis, airsacculitis, and perihepatitis. microscopically, there were fibrinous pericarditis and airsacculitis, myocardial necrosis, necrotizing hepatitis, splenic necrosis with reticuloendothelial cell hyperplasia, interstitial ... | 1992 | 1602582 |
| gastrointestinal helminths of the cetaceans phocoena spinipinnis (burmeister, 1865) and cephalorhynchus eutropia (gray, 1846) from the southern coast of chile. | gastrointestinal helminths of the cetaceans cephalorhynchus eutropia and phocoena spinipinnis accidentally entangled in gillnets off the coast of queule, chile, were identified from 1989 to 1990. pseudoterranova sp., polymorphus (polymorphus) cetaceum and synthesium tursionis occurred in both cetaceans. additionally, anisakis sp. and braunina cordiformis were found in c. eutropia, and anisakis simplex was identified from p. spinipinnis. the species with the highest prevalence and mean intensity ... | 1992 | 1602588 |
| the d1 protein of the photosystem ii reaction-centre complex accumulates in the absence of d2: analysis of a mutant of the cyanobacterium synechocystis sp. pcc 6803 lacking cytochrome b559. | the reaction center core of photosystem ii, a multiprotein membrane bound complex, is composed of a heterodimer of two proteins, d1 and d2. a random mutagenesis technique was used to isolate a photosystem ii deficient mutant, cp6t16, of the unicellular cyanobacterium, synechocystis sp. pcc 6803. nucleotide sequence analysis showed that the primary lesion in cp6t16 is an ochre mutation introducing a translational stop codon in the psbe gene, encoding the alpha-subunit of cytochrome b559, an integ ... | 1992 | 1602969 |
| regional activity of galactose-1-phosphate uridyltransferase in rat brain. | the sp act of galactose-1-phosphate uridyltransferase has been measured in individual regions of adult rat brain to see if site-specific differences in enzyme activity can aid in the understanding of brain abnormalities observed in well-treated galactosemic patients. the sp act in the cerebellum, brain stem, and midbrain were higher than in the cortex, hippocampus, and striatum. activity in the cerebellum was 2-fold greater than that found in the cortex. steady state levels of mrna of the enzyme ... | 1992 | 1603630 |
| neuropeptides in human salivary (submandibular and parotid) glands. | the existence, distribution and density of various neuropeptides in human submandibular and parotid glands were investigated using immunocytochemistry and radioimmunoassay. numerous nerve fibers containing vasoactive intestinal polypeptide (vip) and peptide histidine methionine (phm), or neuropeptide y (npy) and c-flanking peptide of npy (cpon) immunoreactivities (ir) were found in close association to acini, ducts and blood vessels. only few calcitonin gene-related peptide (cgrp)- and substance ... | 1992 | 1605004 |
| ultrastructural changes in contralateral superior vestibulo-ocular neurons one year after vestibular neurectomy in the cat. | twenty contralateral superior vestibulo-ocular neurons (svon) from 3 cats were studied morphologically one year after a right vestibular neurectomy. eighteen svon contained a smooth or slightly crenated nuclear membrane, a 63% loss of synaptic profiles (sp) and a 22% decrease in size compared to control svon. two cells contained nuclear membrane invaginations, a 40% loss of sp and a 31% size decrease compared to control svon. the volume fractions of rough endoplasmic reticulum and ribosomes were ... | 1992 | 1605013 |
| evaluation of prenatal aldrin intoxication in rats. | the aim of the present study was to evaluate the impact of prenatal exposure to low doses of aldrin on physical and behavioral developments of rats (1-21 days old). to detect the possible persistent adversities produced by this exposure, the animals were also tested when adults (90 days old). plasma determinations of both aldrin and its metabolite dieldrin and histopathological evaluations of brain slices were also performed in adult animals. pregnant female rats were subcutaneously (s.c.) treat ... | 1992 | 1605732 |
| estrogen platinum-diamine complexes: preparation of a non-steroidal estrogen platinum-diamine complex labeled with platinum-191 and a study of its binding to the estrogen receptor in vitro and its tissue distribution in vivo. | we have prepared in radiolabeled form (platinum-191) a non-steroidal estrogen platinum-diamine complex (pt-diamine complex) that is reported to have selective cytostatic activity in estrogen receptor positive mouse mammary tumors. we then studied the interaction of this metal radiolabeled complex with the estrogen receptor in vitro and its distribution in immature rats in vivo. the radiolabeled complex was prepared by incubation of the non-steroidal estrogen diamine with [191pt](ii)cl(-2)4 (t 1/ ... | 1992 | 1606047 |
| [usefulness of studies of susceptibility of ampicillin, carbenicillin, cefalotin and colistin as a presumptive identification system for the aeromonas sp. group]. | we have studied the restricted antibiotic susceptibility tests for 180 strains belonging to mesophilic aeromonas sp. isolated from stools samples. strains has been identified as follows: a. caviae (64.6%), a. hydrophila (17.7%) and a. sobria (17.7%). antibiotics tested were ampicillin (10 micrograms), carbenicillin (100 micrograms), cephalotin (30 micrograms) and colistin (10 micrograms), by means of agar diffusion tests (disk-plate). we identified four different patterns of antibiotic susceptib ... | 1992 | 1606227 |
| characterization of the rat pulmonary surfactant protein a promoter. | the expression of the pulmonary surfactant protein a (sp-a) is developmentally regulated and controlled by several hormones. in an attempt to characterize cis-acting elements involved in the regulation of sp-a expression, we have cloned the 5' flanking sequence of the rat sp-a gene. the promoter region contains a tata box but no caat box. the transcription start site has been identified by anchored polymerase chain reaction and s1 nuclease mapping of the mature and precursor transcripts. s1 mapp ... | 1992 | 1606951 |
| neuropeptide content of purified rat brain cholinergic synaptosome subpopulations. | cholinergic synaptosomes from rat cerebral cortex were isolated by a magnetic immunoaffinity technique, i.e. immunomagnetophoresis. this subpopulation was extracted and subjected to radioimmunoassay for 4 neuropeptides:neuropeptide y (npy); vasoactive intestinal peptide (vip); substance p (sp); and somatostatin (srif). three of the 4 neuropeptides were enriched in the sorted fraction compared with the mother fraction with respect to the cytosolic marker lactate dehydrogenase (ldh). the most enri ... | 1992 | 1608551 |
| specific oligosaccharide form of the rhizobium meliloti exopolysaccharide promotes nodule invasion in alfalfa. | rhizobium meliloti strain su47 produces both high molecular weight (hmw) and low molecular weight (lmw) forms of an acidic exopolysaccharide, succinoglycan. genetic studies have shown that succinoglycan is required for alfalfa root nodule invasion. we found that lmw succinoglycan, when applied exogenously to alfalfa roots, restored nodule invasion to exoa, exob, exof, and exoh mutants. nodule initiation signals were not involved, since lmw succinoglycan from r. meliloti nodd1d2d3 and noda mutant ... | 1992 | 1608972 |
| in vitro ruminal metabolism of larkspur alkaloids. | larkspur (delphinium sp) poisoning of range cattle is a serious, recurring problem in the western united states. numerous stratagems have been recommended to ameliorate larkspur intoxication, but none are completely effective. previous studies in this laboratory indicated bovine ruminal microflora are capable of chemically modifying delphinium alkaloids. research reported herein was undertaken to further evaluate whether differences in ovine and bovine rumen metabolism might explain differences ... | 1992 | 1609485 |
| biodegradation of the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) by purified pentachlorophenol hydroxylase and whole cells of flavobacterium sp. strain atcc 39723 is accompanied by cyanogenesis. | a pentachlorophenol (pcp)-degrading flavobacterium sp. (strain atcc 39723) degraded bromoxynil with the production of bromide and cyanide. no aromatic intermediates were detected in the spent culture fluid. the cyanide produced upon bromoxynil metabolism was inhibitory to the flavobacterium sp. whole cells degraded pcp more rapidly than they did bromoxynil. bromoxynil metabolism and pcp metabolism were coinduced, either substrate serving as the inducer. purified pcp hydroxylase degraded bromoxyn ... | 1992 | 1610174 |
| construction of a 3-chlorobiphenyl-utilizing recombinant from an intergeneric mating. | recombinant pseudomonas sp. strain cb15, which grows on 3-chlorobiphenyl (3cb), was constructed from pseudomonas sp. strain hf1, which grows on 3-chlorobenzoate, and from acinetobacter sp. strain p6, which grows on biphenyl, by using a continuous amalgamated culture apparatus. dna from strains cb15 and hf1 hybridized very strongly to each other, while hybridization between both parental strains, hf1 and p6, was negligible. however, dna from the recombinant cb15 hybridized moderately to strongly ... | 1992 | 1610186 |
| cloning of the guinea pig 5-lipoxygenase gene and nucleotide sequence of its promoter. | the guinea pig 5-lipoxygenase (5-lo) gene and its promoter were cloned from a guinea pig genomic dna library. sequencing analysis of the guinea pig promoter revealed that expression of the 5-lo gene in this rodent is probably governed by cis acting nucleotide sequences quite similar to the human gene. nucleotide sequences that bind factors like sp-1, ap-2, nf-kb and c-ha-ras were identified in the guinea pig 5-lo promoter region. | 1992 | 1610345 |
| a hybrid ribulosebisphosphate carboxylase/oxygenase enzyme exhibiting a substantial increase in substrate specificity factor. | two hybrid ribulose-1,5 bisphosphate carboxylase/oxygenase (rubisco) enzymes were constructed using rubisco small subunit genes (rbcs) from two eucaryotic marine organisms, cylindrotheca sp. n1 and olisthodiscus luteus, cloned downstream of the rubisco large subunit gene (rbcl) of the cyanobacterium synechococcus pcc 6301. the expression products synthesized by escherichia coli jm107 (pvtac223 and panoli) were purified and examined by polyacrylamide gel electrophoresis and compared to the purifi ... | 1992 | 1610800 |
| isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from pseudomonas sp. strain cbs-3. | the three genes encoding the 4-chlorobenzene dehalogenase polypeptides were excised from a pseudomonas sp. cbs-3 dna fragment and separately cloned and expressed in escherichia coli. the three enzymes were purified from the respective subclones by using an ammonium sulfate precipitation step followed by one or two column chromatographic steps. the 4-chlorobenzoate:coenzyme a ligase was found to be a homodimer (57-kda subunit size), to require mg2+ (co2+ and mn2+ are also activators) for activity ... | 1992 | 1610806 |
| the structure of photosystem i from the thermophilic cyanobacterium synechococcus sp. determined by electron microscopy of two-dimensional crystals. | the structure of the photosystem i (ps i) complex from the thermophilic cyanobacterium synechococcus sp. has been investigated by electron microscopy and image analysis of two-dimensional crystals. crystals were obtained from isolated ps i by removal of detergents with bio-beads. after negative staining, either single layers or two superimposed layers with a rotational different orientation were observed. the layers have a rectangular unit cell of 16.0 x 15.0 nm, which contains two ps i monomers ... | 1992 | 1610871 |
| etiology and antibiotic susceptibility of bloodstream streptococcus sp. | 249 episodes of streptococcus bacteraemia in hospitalized patients were evaluated for both clinical and microbiological features. specification of the isolates demonstrated that infective endocarditis was predominantly associated with streptococcus sanguis and streptococcus bovis whereas streptococcus pyogenes and streptococcus milleri were the most common cause of local and/or systemic infections. in vitro-susceptibility tests towards 9 selected antibiotics proved that beta-lactam antibiotics a ... | 1992 | 1611209 |
| av3v lesion suppresses the pressor, dipsogenic and natriuretic responses to cholinergic activation of the septal area in rats. | in the present study we investigated the effect of anteroventral third ventricle (av3v) lesion on pressor, dipsogenic, natriuretic and kaliuretic responses induced by the injection of carbachol (a cholinergic agonist) into the medial septal area (msa) of rats. male rats with sham or av3v lesion and a stainless-steel cannula implanted into the msa were used. carbachol (2 nmol) injected into the msa in sham lesion rats produced pressor (43 +/- 2 mmhg), dipsogenic (9.6 +/- 1.2 ml/h), natriuretic (5 ... | 1992 | 1611511 |
| analysis of porcine gastric mucus glycoprotein added to a culture medium of streptomyces sp. oh-11242 as the only source of carbon. | 1. in the process of obtaining the degradation enzymes of mucus glycoprotein, porcine gastric mucus glycoprotein (pgm), added as the only source of carbon, was removed from the culture medium of streptomyces sp. oh-11242 [iwase et al. (1988) biochem. biophys. res. commun. 151, 422-428] and analysed. 2. the amino acid and carbohydrate compositions of porcine gastric mucus glycoprotein (pgm-m) recovered from a culture medium were similar to those of original pgm. 3. however, the elution profile of ... | 1992 | 1611883 |
| the isolation and partial characterization of trypsinogen, pancreatic secretory trypsin inhibitor and multiple forms of chymotrypsinogen and trypsin from the pancreas of the ostrich (struthio camelus). | 1. psti, two chymotrypsinogens and two trypsins were purified to homogeneity by acid extraction, salt fractionation, sp-sephadex c-50 chromatography and rp-hplc. 2. a third chymotrypsinogen, a trypsinogen and another trypsin were purified using an alkaline extraction procedure, followed by trasylol- and benzamidine-sepharose affinity chromatography and hydroxylapatite chromatography. 3. the enzymes differed in amino acid composition as well as in specific activities towards synthetic amidase and ... | 1992 | 1612178 |
| identification of casuarina-frankia strains by use of polymerase chain reaction (pcr) with arbitrary primers. | free-living n2-fixing frankia strains isolated from casuarina sp. were investigated for genomic polymorphism. we used six 10-mer oligonucleotides as single arbitrary primers (ap) for the polymerase chain reaction (pcr) in order to amplify random dna fragments in the genome of free-living frankia strains. agarose-gels of the amplified genomic dna revealed that two of the six arbitrary primers showed polymorphism in the eight different frankia genomes. analysis of the ap-pcr products showed 9 poly ... | 1992 | 1612411 |
| the expanded horizon for microbial metabolites--a review. | modern screening methods have been used for a variety of new natural products. by taking advantage of the side effects of erythromycin, a derivative, em523, and several related substances (motilides) have been synthesized. these compounds are agonists of the peptide hormone, motilin. by screening for microbial metabolites which may substitute for biologically active peptides, we discovered lactacystin. it has nerve growth-factor-like activity and induces differentiation in mouse neuroblastoma ne ... | 1992 | 1612429 |
| ca-dependent k channels in smooth muscle cells permeabilized by beta-escin recorded using the cell-attached patch-clamp technique. | using the cell-attached patch-clamp technique, the activity of single, ca-dependent k channels was recorded in single smooth muscle cells permeabilized by beta-escin. the conductance and the relationship between the open probability of the channels and pca recorded in permeabilized cells were very similar to those obtained in excised inside-out patches. at pca 7, application of 30 microm acetylcholine (ach) or 0.1 microm substance p (sp) together with 1 mm guanosine 5'-trisphosphate to permeabil ... | 1992 | 1614818 |
| photoperiod and gonadal hormones influence odor preferences of the male meadow vole, microtus pennsylvanicus. | male meadow voles housed in a long photoperiod (14 h light/day, lp) preferred female to male odors, whereas males maintained in a short photoperiod (10 h light/day, sp) did not display preferences for odors of either sex. these odor-preference patterns matched those of free-living males during spring and autumn, respectively. the preference of lp male voles for female over male odors was eliminated by gonadectomy and reinstated by treatment with testosterone. in sp males, although gonadectomy di ... | 1992 | 1615048 |
| rabbit lung surfactant protein a gene: identification of a lung-specific dnase i hypersensitive site. | expression of the gene encoding pulmonary surfactant protein a, sp-a, is lung specific and developmentally and hormonally regulated. previously, we observed that sp-a gene transcription is initiated in fetal rabbit lung after day 21 of gestation and reaches maximal levels by day 28. in the present study, a cdna specific for rabbit sp-a was used to isolate the sp-a gene from a rabbit genomic library. a 7.6-kb fragment containing the entire structural gene and approximately 380 bp of 5'-flanking d ... | 1992 | 1616051 |
| effects of tnf-alpha and phorbol ester on human surfactant protein and mnsod gene transcription in vitro. | tumor necrosis factor-alpha (tnf-alpha) and the phorbol ester, 12-o-tetradecanoyl-phorbol-13-acetate (tpa) decrease the synthesis of surfactant proteins association with decreased sp-a and sp-b mrna. nuclear run-on assays were utilized to test whether the inhibitory effects of tnf-alpha and tpa were associated with changes in surfactant protein gene transcription. sp-a gene transcription was inhibited by both tnf-alpha and tpa as assessed by nuclear run-on assays. inhibitory effects of both agen ... | 1992 | 1616052 |
| binding, uptake, and localization of surfactant protein b in isolated rat alveolar type ii cells. | this study reports the ability of rat alveolar type ii cells to internalize mature bovine surfactant protein b (sp-b) in vitro. isolated type ii cells were incubated with labeled sp-b, and binding and internalization were studied biochemically and morphologically. biochemical analyses demonstrated a time-dependent association of 125i-labeled sp-b with type ii cells; binding steadily increased through 4 h and then remained constant through 20 h of incubation. the association of [3h]sp-b with type ... | 1992 | 1616054 |
| effects of surfactant apolipoproteins on liposome structure: implications for tubular myelin formation. | tubular myelin is one of several forms of lung surfactant and may play an important role in its surface activity. to determine possible mechanisms of tubular myelin formation, we studied the effects of purified surfactant proteins (sp-a, sp-b, and sp-c) on large unilamellar dipalmitoylphosphatidylcholine-egg phosphatidylglycerol (7/3; wt/wt) liposomes. we studied different types of membrane interaction induced by the apolipoproteins and correlated these with the observed changes in ultrastructur ... | 1992 | 1616057 |
| surfactant protein a metabolism in preterm ventilated lambs. | surfactant protein a (sp-a) metabolism was studied in vivo in 33 preterm ventilated lambs at 138 +/- 1 days gestational age by measuring recoveries of exogenously administered surfactant containing both radiolabeled sp-a and labeled saturated phosphatidylcholine (sat pc) given via the trachea at birth. endogenously secreted sp-a was also labeled with [35s]methionine and followed over 24 h. the exogenously labeled sp-a left the alveolar pool more rapidly than did sat pc over the first 5 h of life ... | 1992 | 1616059 |
| differential extraction for the rapid purification of bovine surfactant protein b. | surfactant protein b (sp-b), a peptide found in organic solvent extracts of mammalian surfactant, has been isolated from surfactant previously by column chromatography and/or preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds/page). we have developed a method for isolation of sp-b from bovine surfactant utilizing differential organic extraction. dried surfactant, isolated from lavage of excised cow lungs, was delipidated by extraction with diisopropyl ether-butanol (3:2). ... | 1992 | 1616060 |
| peptidase b polymorphism in cattle erythrocytes. | starch gel electrophoresis and histochemical staining with l-leucylglycyl-glycine revealed genetic polymorphism in peptidase b in cattle erythrocytes. genetic analysis of 164 full families indicated that the three phenotypes observed are the product of an autosomal locus with two codominant alleles, pepb1 and pepb2. a sample consisting of 2630 individuals representing 5 zebu, 13 european, and 2 crossbred breeds was studied. this polymorphism was observed in all zebu and crossbred breeds. the fre ... | 1992 | 1616480 |
| effects of lung surfactant proteolipid sp-c on the organization of model membrane lipids: a fluorescence study. | lipid-protein interactions of pulmonary surfactant-associated protein sp-c in model dppc/dppg and dppc/dppg/eggpc vesicles were studied using steady-state and time-resolved fluorescence measurements of two fluorescent phospholipid probes, nbd-pc and nbd-pg. these fluorescent probes were utilized to determine sp-c-induced lipid perturbations near the bilayer surface, and to investigate possible lipid headgroup-specific interactions of sp-c. the presence of sp-c in dppc/dppg membrane vesicles resu ... | 1992 | 1616924 |
| neisseriaceae and moraxella sp.: the role of related microorganisms associated with conjunctivitis in the newborn. | | 1992 | 1616970 |
| two c4-dicarboxylate transport systems in rhizobium sp. ngr234: rhizobial dicarboxylate transport is essential for nitrogen fixation in tropical legume symbioses. | to investigate the role of dicarboxylate transport in nitrogen-fixing symbioses between rhizobium and tropical legumes, we made a molecular genetic analysis of the bacterial transport system in rhizobium sp. ngr234. this braod host range strain fixes nitrogen in association with evolutionarily divergent legumes. two dicarboxylate transport systems were cloned from rhizobium ngr234. one locus was chromosomally located, whereas the other was carried on the symbiotic plasmid (psym) and contained a ... | 1992 | 1617199 |
| interaction of antileukemia agents adriamycin and daunomycin with sphinganine on the differentiation of human leukemia cell line hl-60. | a slight induction of granulocytic differentiation of hl-60 cells occurred after treatment with antileukemia chemotherapeutic agents adriamycin (adm) and daunomycin (dm). addition of an inhibitor (sphinganine, sp) of protein kinase c (pkc) enhanced 2-4-fold the adm or dm-induced differentiation. this phenomenon was accompanied by a slightly augmented antiproliferative effect. the enhancement of differentiation induction in these treatments seemed to be absolute, since the combination treatment ( ... | 1992 | 1617630 |
| high efficiency transformation of fusarium solani f. sp. cucurbitae race 2 (mating population v). | a cosmid vector, suitable for library construction and dna transformation in filamentous fungi, has been constructed and a reliable and highly efficient peg-mediated dna transformation system for f. solani f. sp. cucurbitae, based on resistance to hygromycin b, has been developed for use with this vector. this transformation system yielded 10(4) transformants per micrograms of dna when using 10(7) protoplasts. factors important in achieving high efficiency included: the maintenance of an osmotic ... | 1992 | 1617735 |
| in vitro activity of a new broad-spectrum, beta-lactamase-stable oral cephalosporin, cefixime, in comparison with other drugs, against haemophilus influenzae, haemophilus parainfluenzae, moraxella catarrhalis and streptococcus pneumoniae. | cefixime, a new orally absorbed iminomethoxyaminothiazolyl cephalosporin, was tested against some microorganisms involved in upper and lower respiratory tract infections such as haemophilus (influenzae and parainfluenzae), moraxella catarrhalis and streptococcus pneumoniae, isolated in the period from november 1990 to april 1991. its activity was compared to nine other antimicrobial agents: erythromycin, trimethoprim-sulfamethoxazole, ampicillin, amoxicillin-clavulanate, cefaclor, ceftazidime, c ... | 1992 | 1618002 |
| single-fibre and whole-nerve responses to clicks as a function of sound intensity in the guinea pig. | this paper describes a study of the intensity dependence of click-evoked responses of auditory-nerve fibres in relation to the simultaneously recorded compound action potential (cap). condensation and rarefaction clicks were presented to normal hearing guinea pigs over an intensity range of 60 db. the recorded poststimulus time histograms (psths) were characterized by the latency (tp), amplitude (ap) and synchronization (sp) of their dominant peak, parameters that are particularly important for ... | 1992 | 1618706 |
| induction of low density lipoprotein receptor and a transcription factor sp-1 by tumor necrosis factor in human microvascular endothelial cells. | we have previously reported that tumor necrosis factor-alpha (tnf-alpha) enhances expression of interleukin-6, collagenase, plasminogen activator inhibitor-1, and basic fibroblast growth factor genes in human omental microvascular endothelial (home) cells in culture. in this study, we found that treatment of home cells with tnf-alpha or interleukin-1 (il-1) caused enhanced expression of low density lipoprotein (ldl) receptor. a few-fold increase in both ldl binding activity and the receptor mrna ... | 1992 | 1618817 |
| segmental distribution and peptide content of primary afferent neurons innervating the urogenital organs and colon of male rats. | many visceral afferent neurons contain peptides, which have been proposed as histochemical markers for nerve pathways of particular targets or as transmitter candidates. the former possibility was investigated in the present study. primary afferent neurons which project to the urinary bladder, distal colon or penis of rats, and the colon of cats were labelled with retrogradely transported fluorescent dyes (fast blue, true blue, or fluoro gold). one to six weeks after dye injection into the organ ... | 1992 | 1619047 |
| prevalence and risk factors for taenia solium taeniasis and cysticercosis in humans and pigs in a village in morelos, mexico. | in a mexican village in which taenia solium infection was known to be endemic, we selected a cluster sample of 368 households (21% of the total) for demographic, environmental, and diagnostic surveys, and medical histories for taeniasis and cysticercosis. coproparasitologic studies of 1,531 participants revealed infection by taenia sp. in four (0.3%) individuals; however, 5.8% of the respondents reported a history of having passed tapeworm proglottids in feces. of 1,552 human serum specimens, 10 ... | 1992 | 1621892 |
| purification and characterization of a new bacteriocin isolated from a carnobacterium sp. | a bacteriocin-producing carnobacterium sp. was isolated from fish. the bacteriocin, termed carnocin ui49, was purified to homogeneity by a four-step purification procedure, including hydrophobic interaction chromatography and reverse-phase chromatography. carnocin ui49 has a bactericidal mode of action. it was shown to be heat tolerant and stable between ph 2 and 8. at ph above 8, carnocin ui49 was rapidly inactivated. amino acid analysis revealed a composition of about 35 to 37 amino acids in a ... | 1992 | 1622206 |
| characteristics of a new cellulolytic clostridium sp. isolated from pig intestinal tract. | gram-positive, spore-forming, motile, cellulolytic rods were isolated from 10(7) dilutions of pig fecal samples. the pigs had previously been fed pure cultures of the ruminal cellulolytic organism clostridium longisporum. isolates formed terminal to subterminal spores, and a fermentable carbohydrate was required for growth. besides cellulose, the isolates utilized cellobiose, glycogen, maltose, and starch. however, glucose, fructose, sucrose, pectin, and xylose were not used as energy sources. m ... | 1992 | 1622234 |
| cloning and expression of the cryivd gene of bacillus thuringiensis subsp. israelensis in the cyanobacterium agmenellum quadruplicatum pr-6 and its resulting larvicidal activity. | a mosquitocidal cyanobacterium has been developed by introducing the mosquito-toxic cryivd gene from bacillus thuringiensis subsp. israelensis into the unicellular cyanobacterium agmenellum quadruplicatum pr-6 (synechococcus sp. strain pcc 7002). the cryivd gene was introduced into the cyanobacterium on a derivative of the pr-6 expression vector paqe19 delta sal in which the cryivd gene was translationally fused to the initial coding sequence of the highly expressed pr-6 cpcb gene. coomassie blu ... | 1992 | 1622235 |
| effect of bacterial extracellular polymers on the saturated hydraulic conductivity of sand columns. | columns were packed with clean quartz sand, sterilized, and inoculated with different strains of bacteria, which multiplied within the sand at the expense of a continuous supply of fresh nutrient medium. the saturated hydraulic conductivity (hcsat) of the sand was monitored over time. among the four bacterial strains tested, one formed a capsule, one produced slime layers, and two did not produce any detectable exopolymers. the last two strains were nonmucoid variants of the first two. only one ... | 1992 | 1622240 |
| naphthalene degradation via salicylate and gentisate by rhodococcus sp. strain b4. | rhodococcus sp. strain b4, isolated from a soil sample contaminated with polycyclic aromatic hydrocarbons, grows with naphthalene as the sole source of carbon and energy. salicylate and gentisate were identified as intermediates in the catabolism of naphthalene. in contrast to the well-studied catabolic pathway encoded by the nah7 plasmid of pseudomonas putida, salicylate does not induce the genes of the naphthalene-degradative pathway in rhodococcus sp. strain b4. the key enzymes of naphthalene ... | 1992 | 1622263 |
| reduction of nitroaromatic compounds mediated by streptomyces sp. exudates. | exudates from streptomyces griseoflavus tü 2484 effectively mediated electron transfer between hydrogen sulfide and various nitrobenzenes. in general, pseudo-first-order kinetics were observed, except for the initial phase of the reaction at higher ph values. under fixed ph and dh conditions, linear free energy relationships were found between the logarithms of the reaction rate constants and the one-electron reduction potentials of the nitroaromatic compounds. no competition was observed betwee ... | 1992 | 1622270 |
| incubation of water samples containing amoebae improves detection of legionellae by the culture method. | some protozoans isolated from aquatic habitats, including domestic water supplies, can support the intracellular replication of autochthonous legionellae in vitro. we studied the effect of incubating water samples containing amoebae on the sensitivity of culture for legionellae. samples collected during investigations of legionellosis epidemics and shown by conventional culture procedures to contain amoebae, but not legionellae, were incubated at 35 degrees c and replated. legionellae were recov ... | 1992 | 1622278 |
| aerobic and anaerobic bacteriology of intracranial abscesses. | the bacteriologic and clinical findings of 39 pediatric patients with intracranial abscess are presented. twenty-three children presented with brain abscess and 16 with subdural empyema. predisposing conditions were present in all instances. sinusitis was present in 25 children and 4 patients each had chronic otitis media, dental abscess, and congenital heart disease. the abscess was located in the frontal area in 14 patients, parietal in 13, and temporal in 12. anaerobic organisms alone were re ... | 1992 | 1622518 |