| [the etiologic significance of ultrafine forms of the causative agent of tuberculosis in the development of sarcoidosis of the respiratory organs]. | pathological material, i. e. bronchoalveolar lavage fluid (balf) and blood plasma from patients with various pathological processes in the lungs was studied. eighty seven patients with sarcoidosis of the respiratory organs were subjected to complex microbiological investigation and ultrasmall forms of tubercle bacilli were detected in balf of 67 (77 per cent) of them. histological examination revealed alveolitis and granulomas of lymphoid-macrophagal nature in the lungs of the experimental anima ... | 1989 | 2505249 |
| 3-(2-imidazo[4,5-b]pyridine)propionic acid and some of its derivatives with suspected tuberculostatic activity. | the reactions of corresponding 2,3-diamino-pyridines with succinic anhydride were carried out. 3-(2-imidazo[4,5-b]pyridine)propionic acid and its n-1 and n-3 methyl derivatives were isolated. the acids obtained were converted into the esters, amides, nitriles, thioamides, amidoximes and hydrazides. some of the synthesized compounds were tested for tuberculostatic activity. | 1989 | 2505276 |
| [tuberculosis and hiv infection: study of 50 cases]. | the clinical, microbiological, radiological and sonographical finding of 50 patients with tuberculosis and hiv infection are herewith described. 44 of the patients were men and 46 were intravenous drug addicts. severe respiratory symptoms and peripheral pathological adenopathies were the most encountered manifestations. those patients with tuberculosis and negative hiv titers (p less than 0.005) commonly showed lung miliary signs and mediastinal adenopathies when compared to others. abdominal ad ... | 1989 | 2505338 |
| tenosynovitis of the hand: a forgotten manifestation of tuberculosis. | once the most common form of chronic tenosynovitis of the hand, tenosynovitis due to mycobacterium tuberculosis has become rare. descriptions of this clinical entity can no longer be found in medical textbooks. because of the rarity of this condition, diagnosis is frequently delayed. we present a case and review the presentation, diagnostic criteria, and treatment of tuberculous tenosynovitis. the relation of this infection to antecedent trauma and dupuytren's contracture is discussed. m. tuberc ... | 1989 | 2505354 |
| the modern mycobacteriology laboratory. how it can help the clinician. | the mycobacteriology laboratory provides the information necessary to diagnose mycobacteriosis and to suggest proper patient management. when the definite diagnosis of disease due to m. tuberculosis is made on the basis of the laboratory result, contact follow-up studies should continue and the patient may be considered infectious if the bacilli were isolated from the sputum. a report of another mycobacterium species suggests that a contact follow-up is not necessary, that the patient need not b ... | 1989 | 2505961 |
| drug-resistant tuberculosis. | multiply resistant tuberculosis is on the rise throughout the world. it poses the risk that an increasing percentage of patients will have disease that cannot be cured in economically limited nations and, thus, resistant tubercle bacilli will be spread in an exponential manner. for such patients in the united states, aggressive chemotherapy, coupled with surgery in cases of localized disease, is the best hope for cure. | 1989 | 2505962 |
| new antimycobacterial agents. | development of new antimycobacterial agents has gained impetus from the frequent occurrence of disseminated m. avium complex infections associated with the aids epidemic and the resurgence of tuberculosis. promising new agents are being developed by modification of existing antimycobacterial agents (for example, aminoglycosides, macrolides, beta-lactams, and rifamycins) and by development of new therapeutic classes of drugs (for example, 4-quinolones). in addition, new drug delivery systems (lip ... | 1989 | 2505963 |
| cloning, sequence determination, and expression of a 32-kilodalton-protein gene of mycobacterium tuberculosis. | we describe the identification of the gene encoding an immunodominant 32-kilodalton (kda) protein of mycobacterium tuberculosis. the 32-kda antigen is abundantly secreted into the culture supernatant of a variety of mycobacteria and appears to be a major stimulant of cellular and humoral immunity against mycobacteria. recombinant clones expressing a 140- or 125-kda beta-galactosidase fusion protein reactive with rabbit polyclonal anti-32 kda protein serum were detected. the corresponding dna seq ... | 1989 | 2506131 |
| limiting dilution analysis of t cell unresponsiveness to mycobacteria in advanced disseminated tuberculosis. | peripheral blood mononuclear cells from patients with advanced disseminated tuberculosis (dis-tb) do not respond to purified protein derivative (ppd) measured as cell proliferation, lymphokine production and interleukin (il)-2 receptor (tac antigen) expression. limiting dilution analysis revealed "multi-hit" curves and low frequencies of ppd-reactive t cells in cultures of dis-tb, and "single-hit" curves and high frequencies of ppd-reactive t cells in cultures of patients with localized form of ... | 1989 | 2506411 |
| exploratory laparotomy for diagnosis of tuberculous peritonitis. | tuberculous (tb) peritonitis is uncommon but may present at any age and socioeconomic group. we reviewed 14 patients with tb peritonitis diagnosed during a five year period (six were white and eight, pacific islanders). the mean age was 31 years (a range of three to 69 years). symptoms, signs and laboratory and roentgenologic studies were not specific. peritoneal tap and laparoscopic procedures gave positive results of mycobacterium tuberculi in four of seven patients. diagnosis was not suspecte ... | 1989 | 2506655 |
| detection of mycobacterial antigens in cerebrospinal fluid by enzyme-linked immunosorbent assay. | by use of commonly available antibodies against mycobacterium bovis bcg, mycobacterium tuberculosis antigens can be detected by a rapid and sensitive double-antibody sandwich enzyme-linked immunosorbent assay (elisa). the elisa was dose-dependent and capable of detecting as little as 4 ng of antigens. absorbance for 5 patients with confirmed tuberculous meningitis ranged from 0.150 to 0.600 with a mean value of 0.271 +/- 0.190. for 134 non-meningitis control patients and 6 treated tuberculous me ... | 1989 | 2506683 |
| broth culture: the modern 'guinea-pig' for isolation of mycobacteria. | three cases of mycobacterial infection were confirmed in our laboratory by exclusive isolation of the pathogen in middlebrook 7h9 broth medium. the mycobacteria failed to grow in all three cases on three different solid media. in one patient mycobacterium tuberculosis was isolated from a pleural biopsy; a lymph node biopsy from another patient also yielded m. tuberculosis and m. avium-intracellulare was isolated from the spinal fluid of a patient with acquired immunodeficiency syndrome (aids). o ... | 1989 | 2506684 |
| elisa analysis of bactec bottles for the earlier diagnosis of tuberculosis. | enzyme-linked immunosorbent assays (elisa) have enabled earlier identification of mycobacterium tuberculosis (tb) in clinical settings by utilizing both tb antibody and antigen detection. we studied the sensitivity and specificity of elisa detection of tb antigen by using a commercially available anti-bcg antibody in conjunction with bactec 7h12b culture bottles. we compared these results against those obtained with cultures of mycobacterium avium-intracellulare and mycobacterium kansasii. all b ... | 1989 | 2506783 |
| development of a rapid method for determining the susceptibility of mycobacterium tuberculosis to isoniazid using the gen-probe dna hybridization system. | a rapid test was developed for determining the susceptibility of mycobacterium tuberculosis to isoniazid by using nucleic acid hybridization. the method was based on quantification of total mycobacterial rrna hybridized to a 125i-labeled dna probe in the absence and presence of various concentrations of isoniazid. the radioactive hybridized complex was isolated by adsorption to hydroxyapatite crystals and measured in a gamma counter. the susceptibilities of four reference strains and 20 clinical ... | 1989 | 2506802 |
| drug resistance of m. tuberculosis in chile: rates of initial resistance for 1986 and acquired resistance for 1985. | | 1989 | 2506959 |
| synthesis of galactofuranose disaccharides of biological significance. | methyl beta-d-galactofuranoside was readily obtained by tin(iv) chloride-catalyzed glycosylation of penta-o-benzoyl-alpha,beta-d-galactofuranose, followed by debenzoylation with sodium methoxide. glycosylation of 1 with 2,3,5-tri-o-benzoyl-d-galactono-1,4-lactone or with the 6-o-trityl-lactone derivative 5 gave the benzoylated beta-d-galactofuranosyl-(1----6)-d-galactono-1,4-lactone 6 in excellent yield. the structure of disaccharide 6 was confirmed by borohydride reduction to the glycosyl-aldit ... | 1989 | 2507141 |
| [identification of mycobacteria and measurement of whole cell fatty acids using a gas chromatography computer system]. | the technique of combination gas chromatography with computer used in measuring fatty acids of 28 species standard mycobacteria was introduced in this study. the data of components of fatty acids in this genus and gc graphs with satisfaction were gained. several peaks with good reproducibility were distinguished automatically, based on which, m. tuberculosis, m. bovis and other comment atypical mycobacteria may be separated respectively. it was showed that this method have the characteristics of ... | 1989 | 2507179 |
| role of culture for mycobacteria in fine-needle aspiration diagnosis of tuberculous lymphadenitis. | a total of 390 cases of tuberculous lymphadenitis was subjected to fine-needle aspiration cytology; 100 of the aspirates were subjected to culture for mycobacteria. the overall acid-fast bacilli (afb) positivity in smears was 23.58%, with a maximum positivity of 32.94% in smears with both necrosis and granuloma. the overall rate of isolation of mycobacteria on culture was 35%. mycobacteria were more frequently isolated from caseating lesions (40%) than noncaseating lesions (9%). caseating lesion ... | 1989 | 2507267 |
| characterization of t cell antigens associated with the cell wall protein-peptidoglycan complex of mycobacterium tuberculosis. | mycobacterium tuberculosis cell walls are likely to contain critical t cell ag capable of inducing protective immunity against the development of tuberculosis in animal models. therefore, we characterized cell wall-associated ag that stimulate t lymphocytes in tuberculosis patients and clinically well tuberculin-positive individuals. a protein-peptidoglycan complex isolated from the m. tuberculosis cell wall had potent immunologic activity, evoking pbmc proliferative responses similar to those i ... | 1989 | 2507635 |
| synthesis and characterization of new chromium(iii), vanadium(iv), and titanium(iii) complexes with biologically active isonicotinic acid hydrazide. | new complexes of the type [cr(inh)2cl2]cl.2h2o, vo(inh)2cl2 and tio(inh)2cl, where inh = isonicotinic acid hydrazide, have been prepared. the complexes were characterized by infrared and uv-vis spectroscopy, proton nuclear magnetic resonance (nmr) and elemental analyses, molar conductivity and x-ray powder diffraction measurements. for the cr(iii)-complex, the ligand was coordinated through its carbonyl group and amino nitrogen atom; for v(iv)-complex and ti(iii)-complex, the ligand was coordina ... | 1989 | 2507740 |
| improved elisa with immunoabsorbent-purified mycobacterial antigen for serodiagnosis of tuberculosis. | an antigen was purified from mycobacterium tuberculosis h37ra culture filtrate by immunoabsorbent affinity chromatography with cnbr-activated sepharose 4b column coupled with pooled gamma-globulin fraction from patients with active tuberculosis. the column was washed extensively with pbs and eluted with 3m sodium thiocyanate. peak fractions were pooled and used as coating antigen in an elisa. sera from 86 normal subjects and 54 patients with active tuberculosis were tested against the immunoabso ... | 1989 | 2507785 |
| [evaluation of cycloserine in the treatment of infections caused by nontuberculous mycobacteria viewed from in vitro experiments]. | evaluation of cycloserine as a drug in the treatment of infections caused by nontuberculous mycobacteria was made from in-vitro studies, in which mycobacterium tuberculosis strains were used as the standard of the evaluation. the susceptibility testing to cycloserine was made using ogawa egg medium. bacterial suspensions, 10 mg wet weight/ml, prepared from 10 day-old cultures (m. tuberculosis, 14 day-old cultures) growing on ogawa egg medium were used as the source of inoculation. a 0.02 ml-samp ... | 1989 | 2507817 |
| [molecular cloning and expression of mycobacterium tuberculosis strain aoyama b peptide antigen genes in escherichia coli--a gene encoding 15 kd antigen (at 01)]. | a genomic library of mycobacterium tuberculosis strain aoyama b in escherichia coli k-12 was constructed by cloning sau 3a i cleaved m. tuberculosis aoyama b chromosomal dna into puc18, puc181 or puc182. clones reacting with anti-ppds-rabbit-serum were screened by immunoblotting among 3 x 10(4) clones. seven clones were selected; designating pat 01, pat 101, pat 102, pat 103, pat 104, pat 105 and pat 201. on western blotting, they were shown to produce 15 kd (pat 01, pat 101, pat 105), 18 kd (pa ... | 1989 | 2507818 |
| [a supplement study on the in vitro activity of sulfadimethoxine on mycobacterium avium complex--combined effect with other antituberculosis drugs]. | previously the author observed that sulfadimethoxine is most effective among sulfa drugs against mycobacterium avium complex strains (tsukamura, m.: kekkaku 58: 247-250, 1983) and used this drug in the treatment of pulmonary infection caused by m. avium complex (tsukamura, m.: kekkaku 59: 33-37, 1983). in the present study, some supplemental observations were carried out on the in vitro activity of this drug. in vitro susceptibility testing of sulfadimethoxine by the use of ogawa egg medium was ... | 1989 | 2507819 |
| detection and identification of mycobacteria by amplification of mycobacterial dna. | a 383bp segment of the gene coding for the 65kd mycobacterial antigens from mycobacterium tuberculosis, mycobacterium bovis, mycobacterium avium, mycobacterium paratuberculosis, and mycobacterium fortuitum was amplified using taq polymerase and synthetic oligonucleotide primers and the amplified dnas from four of these species were compared by nucleotide sequencing. although the gene segments from these species showed considerable similarity, oligonucleotide probes which could distinguish m. tub ... | 1989 | 2507865 |
| effect of cyclosporine-a on connective tissue deposition in experimental inflammatory lesions. | the effect of cyclosporine-a, a potent immunosuppressant, on the development of experimental inflammatory lesions in rats was studied both histologically and biochemically. continuous treatment with cyclosporine was begun after the implantation of sponges impregnated with heat-killed mycobacterium tuberculosis and continued for up to 63 days. cyclosporine-a significantly reduced the numbers of neutrophil polymorphonuclear leukocytes and lymphocytes present during both the acute and chronic phase ... | 1989 | 2507884 |
| tuberculous abscess of the pancreas in aids: ct findings. | tuberculous abscess of the pancreas is a rare complication of disseminated tuberculosis. the computed tomographic appearance of a tuberculous pancreatic abscess in a patient with acquired immune deficiency syndrome (aids) is described. one should consider this diagnosis whenever a patient with aids has diffuse abdominal lymphadenopathy. | 1989 | 2507908 |
| [clinical picture and course of newly detected pulmonary tuberculosis in elderly and senile patients, discharging l-forms of mycobacterium tuberculosis]. | one hundred and forty two new cases of pulmonary tuberculosis of elderly and senile ages were observed clinically. in terms of the vegetating mycobacterial population, all the patients were divided into 3 groups. it was shown that pronounced clinical and x-ray signs of pulmonary tuberculosis were characteristic of the patients isolating only bacterial forms of the pathogen (group 1). intensive chemotherapy of the patients of that group was the most efficient. at the same time lethality in that g ... | 1989 | 2508081 |
| [determination of antibodies in blood serum of patients with pulmonary tuberculosis using mycobacterial antigen affinity isolation]. | possible use of radio- and enzyme immunoassays (ria and eia, respectively) in serodiagnosis of tuberculosis with antigen bcg isolated by affinity was studied in comparison to the use of the routine antigen ppd. the specific igg antibodies were determined in serum specimens of 150 patients with various forms of pulmonary tuberculosis and 50 healthy donors. a statistically reliable difference in the efficiency of tuberculosis serodiagnosis (high levels of specificity and sensitivity) by the ria an ... | 1989 | 2508082 |
| [method of combined micro- and macrocultivation of mycobacterium tuberculosis in egg medium using agar gel disks]. | a procedure for combined micro- and macrocultivation of tubercle bacilli in the egg media by using agar gel disks was developed and experimentally tested. the essence of the procedure lies in plating mycobacteria on agar gel disks applied to the egg medium in petri dishes followed by incubation at 37 degrees c for 5-7 days. later on the agar gel disks are removed from the medium and examined microscopically for growth of tubercle bacilli in microcolonies (microcultivation). then the gel disks ar ... | 1989 | 2508083 |
| rapid radiometric method for pyrazinamide susceptibility testing of mycobacterium tuberculosis. | pyrazinamide (pza) is one of the most important drugs in modern chemotherapy of tuberculosis. since pza is active only at an acid ph, testing the susceptibility of mycobacterium tuberculosis to pza is difficult and timeconsuming. therefore, we evaluated the bactec system for rapid testing of pza susceptibility at ph 6. a total of 91 m. tuberculosis strains and 2 different strains of m. bovis bcg were screened for susceptibility to pza. each strain was tested in special 7h12 broth supplemented wi ... | 1989 | 2508202 |
| serum hyaluronate levels reflect disease activity in experimental arthritis models. | serum hyaluronate (ha) levels were measured in rats subjected to adjuvant or type ii collagen induced arthritis. as the arthritic lesions developed, both models showed an increase in serum ha levels of approximately 5 times, from a baseline level of 61-126 ng/ml (range). furthermore a positive correlation was found between ha level and arthritic score. the increase in ha was not related to metabolic impairment, as the half life of serum ha in adjuvant arthritic rats was similar to that of normal ... | 1989 | 2508444 |
| controlled clinical trial of levamisole in short-course chemotherapy for pulmonary tuberculosis. a kenyan/zambian/british medical research council collaborative study. | the effect of adding a once-weekly dose of levamisole for 4 or 8 wk to a 6-month antituberculosis chemotherapy regimen has been studied in a double-blind, placebo-controlled clinical trial. all patients received the same daily chemotherapy regimen of streptomycin, isoniazid, rifampin, and pyrazinamide for 8 wk followed by thiacetazone and isoniazid for 18 wk, the first 10 wk being given fully supervised on an inpatient basis in hospital and the remainder to be self-administered at home. in 504 p ... | 1989 | 2508527 |
| the use of sputum cultures in the evaluation of immigrants classified as tuberculosis suspects. | because of possible deficiencies in the evaluation, based on symptoms and chest roentgenogram review, of new immigrants classified during the visa application process as tuberculosis suspects, a prospective (cohort) and a retrospective (case control) study were done to test the usefulness of routinely obtaining sputum specimens for culture in that setting. in the prospective study, 249 consecutive classified immigrants who were considered on the basis of clinical and roentgenographic findings to ... | 1989 | 2508528 |
| does pyrazinoic acid as an active moiety of pyrazinamide have specific activity against mycobacterium tuberculosis? | the commonly accepted hypothesis explaining the mechanism of action of pyrazinamide (pza) is based on the assumption that pza-susceptible mycobacterium tuberculosis strains produce pyrazinamidase, which hydrolyzes pza to the antibacterial moiety pyrazinoic acid (poa). it is not clear whether poa has specific antimicrobial activity or the inhibition of growth caused by poa is due to its ability to lower the ph of the environment below the limits of tolerance of m. tuberculosis growth. we confirme ... | 1989 | 2508544 |
| [initial resistance to streptomycin, isoniazid, thiacetazone, rifampicin and ethambutol in bacilliferous tuberculosis in maniema, zaire]. | initial resistance to streptomycin, isoniazid, thiacetazone, rifampicin and ethambutol was tested in 102 patients with bacilliferous tuberculosis in kalima, a rural area in eastern zaïre. the initial resistance for at least one of these tuberculostatics was 43%. the highest resistance recorded was for streptomycin (31%). no resistance to rifampicin or ethambutol was found. the practical interest of these findings is discussed. | 1989 | 2508582 |
| sensitivity to "atypical" mycobacteria in high school children in two community health departments. | to evaluate the prevalence of atypical mycobacteria infections in our population, a study was done among students of secondary fifth grade (15-19 years of age) in the community health departments of maisonneuve-rosemont in montreal and of sherbrooke. the tuberculin used was the rt-23 2 t.u. with tween 80 and the sensitins prepared by the statens serum institute of copenhagen from mycobacterium intracellulare (battey) and from mycobacterium kansasii. each student had a tuberculin test on one arm ... | 1989 | 2509059 |
| scalar, dipolar-correlated and j-resolved 2d-nmr spectroscopy of the specific phenolic mycoside of mycobacterium tuberculosis. | two-dimensional chemical shift correlated (cosy), nuclear overhauser (noesy) and j-resolved spectroscopy were used to determine the complete structure of the major triglycosyl dimycocerosyl phenol phthiocerol of the tubercle bacillus (strain canetti) without any other analytical technique. the cosy spectrum of the native glycolipid allowed the composition of the trisaccharide and the location of one methoxyl group to be determined through the assignment of the resonances of the non-anomeric meth ... | 1989 | 2509203 |
| t lymphocytes from healthy individuals with specificity to self-epitopes shared by the mycobacterial and human 65-kilodalton heat shock protein. | the immune response to mycobacterial pathogens comprises a significant percentage of t cells with specificity for a 65-kda heat shock protein (hsp) which is highly conserved in bacteria and man. pbmc were activated in vitro with killed mycobacterium tuberculosis and afterward tested for ctl activity on autologous target cells primed with 1) killed m. tuberculosis, 2) intact recombinant 65-kda hsp of mycobacterium bovis/m. tuberculosis; or 3) tryptic fragments of the recombinant 65-kda hsp. stron ... | 1989 | 2509558 |
| concurrent pulmonary blastomyces dermatitidis and mycobacterium tuberculosis infection in an hiv-1 seropositive man. | | 1989 | 2509572 |
| synthesis and antitubercular activity of n-(2-naphthyl)glycine hydrazide analogues. | n-(2-naphthyl)glycine hydrazide analogues were synthesized and tested for possible in vitro antitubercular activity. n-(2-naphthyl)alanine hydrazide (3), n-methyl-n-(2-naphthyl)glycine hydrazide (5), n-(6-methoxy-2-naphthyl)glycine hydrazide (7), and 3-(2-naphthylamino)butyric acid hydrazide (23) showed potent inhibitory action against mycobacterium tuberculosis h37rv in youman's medium at concentrations ranging from 0.5 to 10.0 micrograms/ml. these compounds showed significant inhibitory action ... | 1989 | 2509708 |
| [thin-needle aspiration biopsy of the lung in the diagnosis of tuberculosis]. | the results of bacteriological examination of materials collected by thin needle aspiration biopsy (tnab) of the lung in patients with suspected cancer are presented. koch's bacilli were detected in 67 cases per 1470 examinations performed for a period of 11 years. positive bacterioscopic results were stated in 20 patients with negative cytological results. in 17 cases drug sensitivity of tubercle bacilli was determined which was important for the treatment tactics. the examination showed that t ... | 1989 | 2510144 |
| [acute hematogenic generalization of tuberculosis caused by l-forms of mycobacteria]. | | 1989 | 2510146 |
| biological activities of some 1,2,4-triazoles and 1,2,4-triazolin-5-ones. | | 1989 | 2510189 |
| [a comparative study of delayed hypersensitivity due to mycobacteria and their isolated protein fractions]. | the role of intracellular protein fractions, isolated from m. tuberculosis virulent strains in the process of the electrophoretic separation, in the development of delayed hypersensitivity reactions has been studied. low reactogenicity of protein fractions has been established on the basis of the development of faint immunopathological reactions in sensitized animals. high-molecular fraction 1 may be used as a sensitive preparation for the differentiation of individual mycobacterial species. | 1989 | 2510431 |
| presumed choroidal tuberculosis in a human immunodeficiency virus infected host. | | 1989 | 2510520 |
| human igg antibodies immunoreacting with specific sulfolipids from mycobacterium tuberculosis. | igg and igm antibodies immunoreacting with sulfolipids from mycobacterium tuberculosis were detected in sera from tuberculosis patients. the method used was an enzyme linked immunoassay (elisa) and the antigens were a 2, 3, 6, 6' tetraacyl trehalose-2'-sulfate (sulfolipid i, sl i) and a 2, 3 diacyl trehalose-2'-sulfate (sulfolipid iv, sl iv). the sl iv antigen was satisfactory for igg assays. the sensitivity and the specificity of the test were, respectively, 59 and 100%; the predictive values o ... | 1989 | 2510752 |
| does reactive arthritis to tuberculosis (poncet's disease) exist? | | 1989 | 2511306 |
| radiometric studies on the use of selective inhibitors in the identification of mycobacterium spp. | radiometric selective inhibition tests were developed and evaluated for the rapid differentiation of mycobacterium spp. both a p-nitrobenzoic acid (pnb) test and a commercially-prepared p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (nap) test successfully differentiated m. tuberculosis and m. bovis from "atypical" mycobacteria or mycobacteria other than tubercle bacilli (mott). thiophene-2-carboxylic acid hydrazide (tch) readily distinguished human m. tuberculosis strains from m. bovis, ir ... | 1989 | 2511320 |
| enzyme-linked immuno-assay for the detection of mycobacterium tuberculosis specific igg antibody in the cerebrospinal fluid in cases of tuberculous meningitis. | the efficacy of enzyme-linked immuno-assay in the detection of igg antibody against mycobacterium tuberculosis in the cerebrospinal fluid of patients suffering from tuberculous meningitis was measured in 50 children consecutively admitted to hospital. the controls were 15 cases of tuberculosis other than of the central nervous system; 24 cases of pyogenic meningitis; 19 cases of neurologic problems but with essentially normal cerebrospinal fluid. the specificity of the test ranged from 93 to 100 ... | 1989 | 2511333 |
| [two cases of long-lasting spcn status]. | the author experienced two model cases of long-lasting spcn (smear positive and culture negative) status. the both cases having no prior chemotherapy, which were suggestive of relationship of spcn to the use of rfp and the presence of huge cavity wherein tuberculous bacilli were most abundant. in these two cases, the laboratory examination showed resistance of the bacillus against inh which was most effective in tuberculous chemotherapy and the bacillus were sensitive against rfp. the spcn conti ... | 1989 | 2511374 |
| [tuberculotic cirrhosis and indices of its activity]. | clinical pictures of tuberculous cirrhosis were studied in 195 persons observed in various groups of dispensary registration. in addition, bacteriological-morphological data relating to 79 operated patients were compared and bacteriological investigations were performed in 145 x-ray negative phthisiologists and 80 patients with nontuberculous diseases of the respiratory organs. it was shown that in the majority of the patients and persons with posttuberculotic cirrhosis the symptomatic process o ... | 1989 | 2511572 |
| [the results of the use of riboxin in the combined treatment of patients with pulmonary tuberculosis]. | studies with infected animals showed that riboxine (inosine) having anabolic, antifibrotic, immunostimulating, antihypoxic and hepatoprotective activities, by the level of its therapeutic effect was not inferior to levamisole or sodium oxybutyrate and exceeded methyluracil. the evidence of the riboxine therapeutic effect mostly correlated with the antihypoxic activity. the use of riboxine at the early stages in the treatment of new cases with infiltrative destructive tuberculosis of the lungs of ... | 1989 | 2511573 |
| [the etiopathogenetic treatment of experimental tuberculosis using preparations with immunomodulating action]. | the effectiveness of the complex chemotherapy of experimental tuberculosis in combination with the remedies of pathogenetic therapy has been studied. the immunomodulating action of levamisole, sodium succinate and the positive effect of rnaase on specific resistance in the complex treatment of guinea pigs infected with tuberculosis have been established. on the basis of the pronounced normalization of the systemic and specific resistance characteristics, as well as the clinical effect, the exped ... | 1989 | 2511711 |
| [glycolipid-patterns of mycobacterium tuberculosis as an aid for sub-typing]. | total lipids were extracted from mycobacterium tuberculosis with chloroform-methanol (2:1), applied on a silica-gel thin-layer plate, and developed with chloroform-methanol-acetone (90:10:5). glycolipids were detected by spraying anthrone-reagent and heating. strain h37rv of m. tuberculosis showed four anthrone-positive spots, namely trehalose-monomycolate, unidentified glycolipid, trehalose-dimycolate and gl-rv, and strain h37ra showed only two spots corresponding to trehalose-monomycolate and ... | 1989 | 2512409 |
| isolation of mycobacterium tuberculosis from faeces. | faeces from patients with myco, tuberculosis in their sputum was decontaminated using naoh, acid and an alkaline precipitation method. treated specimens were diluted, to reduce the number of surviving normal flora, and cultured in liquid selective media. sub-cultures were made on selective agar and lowenstein-jensen medium. both alkaline methods were superior to acid treatment. use of all three methods, dilution and culture, yielded growth of myco, tuberculosis from 90% of patients' faeces, alth ... | 1989 | 2512463 |
| [improvement of the microbiological diagnosis of tuberculosis with an account of the detection of l forms and ultrasmall mycobacteria]. | possible addition of various drugs to the dorozhkova nutrient medium for isolation of l-forms of m. tuberculosis was studied with a purpose of preventing microbial contamination of the cultures. it was shown that the use of malachite green as 1% sterile aqueous solution in an amount of 11 ml per 1 liter of the medium lowered the number of the sprouts from 35 to 4.5 per cent. various microscopic methods were tested to choose the most suitable one for isolating l-forms of m. tuberculosis. differen ... | 1989 | 2512571 |
| [effects of low-frequency ultrasound on mycobacterium tuberculosis]. | the effect of low frequency ultrasound on various strains of tubercle bacilli was studied in 105 experiments. the analysis of the experimental results showed that under the conditions corresponding to those of dissection, sawing and sanation during the surgical operations the ultrasound had a bactericidal action on tubercle bacilli, both sensitive and resistant to antituberculous drugs. in 75 per cent of the objects there was an ultrasound-induced change in the drug resistance of tubercle bacill ... | 1989 | 2512572 |
| diagnosis of tuberculosis: present and future. | current methods used worldwide for diagnosis of tuberculosis (tb) are not markedly different from those used 50 years ago. the standard has been to demonstrate microbiologically the presence of mycobacterium tuberculosis in secretions and/or tissue from the patient. improvements have been made that permit greater sensitivity for the examination of stained smears and for more rapid detection of growth of the organism using radiometric techniques. new methods for diagnosis that may well eliminate ... | 1989 | 2512602 |
| present chemotherapy of tuberculosis. | significant advances in the treatment of tuberculosis have occurred with the introduction of short-course therapy using isoniazid and rifampin throughout therapy. the addition of pyrazinamide to the initial phase of therapy permits shortening of the total duration of treatment to 6 months for most patients. this regimen is well tolerated, results in rapid disappearance of bacilli from the sputum, and has a low rate of relapse. ethambutol should be added to this regimen for any patient with an in ... | 1989 | 2512603 |
| mycobacterium tuberculosis scleritis. | | 1989 | 2512813 |
| frequency of mycobacterium tuberculosis bacteremia in patients with tuberculosis in an area endemic for aids. | mycobacterium tuberculosis bacteremia has recently been reported in patients infected with the human immunodeficiency virus (hiv). at our institution, tuberculosis occurs commonly among patients with and without hiv infection. we sought to determine the frequency of m. tuberculosis bacteremia among patients with newly diagnosed tuberculosis. during a 4-month period, mycobacterial blood cultures were obtained on all identifiable patients with newly diagnosed tuberculosis. fifteen percent (9/59) o ... | 1989 | 2513763 |
| [antimycobacterial antihistaminics]. | the testing of antihistaminic compounds of various chemical classes for antimycobacterial properties is described. a lot of these compounds inhibit the growth of mycobacteria at a concentration of 8-32 micrograms/ml. | 1989 | 2513793 |
| detection of mycobacterial antigens in cerebrospinal fluid by enzyme-linked immunosorbent assay utilizing anti-mycobacterial saline extract antibodies. | using anti-mycobacterial saline extract antibodies, mycobacterium tuberculosis antigens can be detected in cerebrospinal fluid by a highly specific and sensitive double-antibody sandwich enzyme-linked immunosorbent assay (elisa). the elisa was dose-dependent and capable of detecting as little as 6.0 ng of antigens. specificity was demonstrated by negative results (0.063 +/- 0.008) with other bacterial and cryptococcal antigens (n = 9), and maximum cross-reactivity with other mycobacteria was les ... | 1989 | 2514074 |
| [the optimization of the corticosteroid therapy of patients with destructive pulmonary tuberculosis]. | corticosteroids were administered in 120 patients with active pulmonary tuberculosis during the morning hours every other day in association with tuberculostatic agents. it was found that corticosteroids in a dose similar to 15 mg of prednisolone did not influence the efficacy of antibacterial agents while an increase to 20 mg used for not less than 2 months increases the treatment efficacy to 83.3% and over 3 months--to 89.5%. | 1989 | 2514485 |
| [mycobacterium tuberculosis l forms in patients with pulmonary and pleural tuberculosis during antibacterial chemotherapy]. | | 1989 | 2514488 |
| pitfalls with anti-neutrophil cytoplasmic antibodies (anca). | a 39-year-old ugandan student is described presenting with general malaise, fever and a pulmonary infiltrate. open lung biopsy showing infarction and positive anca lead to a diagnosis of wegener's granulomatosis and a treatment with immunosuppressive drugs was instituted. five weeks after admission, however, sputum cultures turned out to be positive for mycobacterium tuberculosis. the importance of anca-interpretation and the possibility of false positive results is discussed. | 1989 | 2515023 |
| inter-bottle transfer of mycobacteria by the bactec 460. | as a result of several episodes of inter-bottle transfer of molds and mycobacteria in our bactec 460 tb system (johnston laboratories, towson, md), we designed some experiments to attempt to reproduce the transfer process. we demonstrated that organism transfer could occur with our instrument during routine use without any indication of instrument malfunction. utilizing a redesigned a1b4 circuit board that extends the time of the needle heating cycle to 85 sec and increases the needle heater max ... | 1989 | 2515024 |
| [production of monoclonal antibodies for mycobacterium tuberculosis aoyama-b and immunological activity of the affinity-purified antigens]. | nine hybridomas of balb/c mouse, producing monoclonal antibodies (mab: mta 1-9) directed to mycobacterium tuberculosis aoyama-b (m. tbc ab), were established. the mabs reacted to m. tbc ab antigens, forming a main band of 32 kd, 24 kd or 19 kd on immunoblotting, except mta 1 that reacted with 58 kd in addition to 32 kd antigen. immunoglobulin isotypes of mabs were igm, igg 1 or igg 2b. on a mode of reactivity of elisa against various mycobacterial antigens, mabs were divided into two categories: ... | 1989 | 2515338 |
| [complications in tuberculous patients--with special reference to secondary mixed infections]. | an attempt was made to elucidate the mechanism which makes pulmonary tuberculosis intractable. patients with pulmonary tuberculosis were divided into the following two groups; one group consisted of patients (n = 37) in which tubercle bacilli converted to negative within 6 months after the institution of anti-tuberculous chemotherapy and remained negative thereafter, and the other group (n = 21) in which tubercle bacilli persisted in spite of long-term chemotherapy. a comparison was made on seru ... | 1989 | 2515339 |
| incidence of inapparent active mycobacterial infections in france detected by an igg serological test based on antigen 60. | a serological test detecting circulating mycobacterial antibodies of the igg type applied to various populations. the test detected no positive cases among old age people in a retirement home. among sexually active women, 3% were positive. this percentage of antigen 60-positive cases was also found among asymptomatic hiv seropositives. the percentage augmented to 5.7% when hospital attendants were analyzed and augmented further to 14% when people known to have had contact with a man suffering fr ... | 1989 | 2515425 |
| [mycobacteriosis--tuberculosis]. | | 1989 | 2515513 |
| [immunology of mycobacteria: various recent contributions to the field of tuberculosis]. | | 1989 | 2515557 |
| drug susceptibility testing in tuberculosis: a comparison of the proportion methods using lowenstein-jensen, middlebrook 7h10 and 7h11 agar media and a radiometric method. | in the present investigation, the conventional drug susceptibility testing procedures using the 1% proportion method on löwenstein-jensen (lj) and middlebrook 7h10/7h11 agar media were compared with a recent radiometric method. the latter using "bactec 460-tb" apparatus and a special broth medium (becton and dickinson diagnostic instrument systems, towson, md, usa) gave drug susceptibility results within a week as compared to 4-5 weeks with the lj method, and 3-4 weeks using 7h10/7h11 agar media ... | 1989 | 2515563 |
| selective inhibition of the mycobacterium tuberculosis complex by p-nitro-alpha-acetylamino-beta-hydroxypropio phenone (nap) and p-nitrobenzoic acid (pnb) used in 7h11 agar medium. | the potential of p-nitrobenzoic acid (pnb) and p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (nap) for discriminating the mycobacterium tuberculosis (m.tb) complex from other mycobacteria was evaluated in cases of clinical isolates. for this purpose, 500 micrograms/ml of pnb and 5 or 10 micrograms/ml of nap were incorporated in 7h11 agar medium, and appropriate dilutions corresponding to about 10(6) viable units were then plated on pnb- or nap-containing media. results were reported as gro ... | 1989 | 2515564 |
| pneumocystis carinii is not a major cause of pneumonia in hiv infected patients in lusaka, zambia. | the clinical occurrence of pneumocystis carinii and mycobacterium tuberculosis was investigated in patients infected with human immunodeficiency virus (hiv) who had clinical pneumonia of unknown aetiology in lusaka, zambia. the results were compared with a similar group of patients in stockholm, sweden. induced sputum samples were stained for pneumocystis by indirect immunofluorescence using monoclonal antibody 3f6 and toluidine blue o. mycobacterial culture and acid fast stain were performed on ... | 1989 | 2515630 |
| slide culture sensitivity tests. | a new method for slide culture sensitivity tests of mycobacterium tuberculosis is described in which smear-positive sputum spread on slides is incubated without prior decontamination in a selective lysed human blood medium. results are available 7 days after setting up the tests and are particularly useful for guiding the treatment of smear-positive patients with a long history of unsuccessful chemotherapy. drug concentrations and definitions of resistance are suggested for tests against isoniaz ... | 1989 | 2515643 |
| drug resistant tuberculous meningitis in the philippines: report of a case. | a fatal case of tuberculous meningitis caused by a multiply-resistant tubercle bacillus is described, the first such case from southeast asia. increased efforts to isolate mycobacterium tuberculosis from the cerebrospinal fluid and determine the extent and pattern of drug resistance are necessary if the high mortality from this disease is to be reduced. | 1989 | 2515644 |
| comparison of the inoculation of mycobacteria by spiral loop and by pipette. | | 1989 | 2515645 |
| gastric washing smears--help or hindrance? | | 1989 | 2515646 |
| resistance to antituberculosis drugs in riyadh, saudi arabia. | the prevalence of resistance to antituberculosis drugs in riyadh was found to be 21.3%, while the rate of primary drug resistance was 11.5%. resistance to isoniazid was the most common (19.4%) followed by rifampicin and streptomycin. the prevalence of primary and acquired rifampicin resistance was 3 and 33.7% respectively. the majority of isolates from patients with acquired resistance to rifampicin were resistant also to isoniazid. | 1989 | 2515647 |
| [antitubercular agents. 45. antimycobacterial thiohydrazide]. | the present paper investigates the activity of thiohydrazides containing a structural fragment of oxalic acid against mycobacterium tuberculosis and mycobacterium kansasii. the drugs included heterocyclic compounds as well. all compounds under study show medium activity. the minimal inhibition concentrations against mycobacterium kansasii are approximately 3 times higher than against mycobacterium tuberculosis. the most effective aromatic compounds have the rm values (silica gel impregnated with ... | 1989 | 2515678 |
| nocardia, mycobacteria and aids. | | 1989 | 2515885 |
| soluble antigen fluorescent antibody test in the serodiagnosis of tuberculous meningitis. | diagnosis of tuberculous meningitis (tbm) is difficult and frustrating. modified soluble antigen fluorescent antibody (safa) test was applied to 40 csf and 32 serum samples from 48 patients to assess the efficacy of this test in the rapid diagnosis of tbm. using this test, false negative results were obtained in 41.6% and 40% of csf and serum samples. false positive results were obtained in 22.2% and 26.7% of csf and serum samples respectively. safa test was not found to be helpful in the sero-d ... | 1989 | 2516087 |
| antigenicity and specificity of selected glycolipid fractions from mycobacterium tuberculosis. | antigenicity of mycobacterium tuberculosis glycolipids polyphthienoyl trehalose (pptr), phenolic glycolipid (pgl-tb1), tetraacyl trehalose-2'-sulphate (sl-i) and diacyl trehalose-2'-sulphate (sl-iv) was examined in rabbits. pptr did not induce production of igg antibodies in rabbits, while pgl-tb1, sl-i and sl-iv glycolipids were efficient in this respect. immune sera raised in rabbits immunoreacted exclusively with the corresponding antigens, which indicated that they were remarkably specific. ... | 1989 | 2516354 |
| tuberculosis and immunology. | | 1989 | 2516444 |
| treatment of pulmonary tuberculosis with short course chemotherapy in south india--5-year follow up. | a controlled clinical trial of three short-course chemotherapy regimens was undertaken in patients with newly diagnosed bacteriologically positive pulmonary tuberculosis. the patients were randomly allocated to receive one of three regimens: rifampicin, streptomycin, isoniazid and pyrazinamide daily for 2 months, followed by streptomycin, isoniazid and pyrazinamide twice weekly for 3 months (r/5) or for 5 months (r/7), or the same regimen as r/7 but without rifampicin (z/7). a bacteriological re ... | 1989 | 2516669 |
| high initial drug resistance in pulmonary tuberculosis in ghana. | between july 1985 and march 1987, initial sensitivity to anti-tuberculosis drugs was studied in patients presenting at the chest clinic of agogo hospital in the forest area of ghana. culture and sensitivity test results were obtained in 99 out of 123 consecutive patients with pulmonary tuberculosis who claim not to have received previous treatment. isoniazid resistance was alarmingly high in the isolates of m. tuberculosis: 21 out of 57 (37%), and thiacetazone resistance was very high in the m. ... | 1989 | 2516670 |
| total and anti-mycobacterial ige levels in serum from patients with tuberculosis and leprosy. | a radioallergosorbent assay (rast) was developed and used to determine the levels of ige antibodies to soluble antigens of mycobacterium tuberculosis (bcg vaccine strain) in sera from patients with tuberculosis and leprosy and in healthy control subjects. total ige levels in the same sera were quantitated with a commercial radioimmunoassay kit. patients with tuberculosis and leprosy had higher total and specific ige levels than the control groups but the overlap of levels in patients and control ... | 1989 | 2516671 |
| the value of routine bronchial aspirate culture at fibreoptic bronchoscopy for the diagnosis of tuberculosis. | we review the results of bronchial aspirate culture for mycobacteria sent routinely in a series of 1734 fibreoptic bronchoscopic procedures. the incidence of tuberculosis in the series was 8.3% (144 cases). of these cases, a positive bronchial aspirate culture was obtained in 119 (82.6%) cases, and it was the exclusive means of diagnosis in 64 (44.4%). in 66% of these cases tuberculosis was not suspected at the time of bronchoscopy. our results suggest that in an area with a high prevalence of t ... | 1989 | 2516672 |
| the interest of follow-up of resistance of the tubercle bacillus in the evaluation of a programme. | | 1989 | 2516748 |
| sensitivity of mycobacterium tuberculosis strains to antituberculosis drugs isolated at dakar university hospital, from 1987 to 1988. | | 1989 | 2516749 |
| an ordinary mortal's guide to the molecular biology of mycobacteria. | | 1989 | 2516750 |
| [2 cases of drug-resistant pulmonary tuberculosis treated with ofloxacin]. | two cases of chronic, cavernous pulmonary tuberculosis with bacilli resistant to several drugs are presented. both patients were treated with a new antituberculous drug ofloxacin. the agent was used with other antituberculous drugs. sputum negativization was seen in only one of the patients, while the in other only a decrease of dissemination was observed. | 1989 | 2516906 |
| [the epidemiological hazard of patients with active pulmonary tuberculosis and undetermined bacterial shedding]. | the degree of the epidemiological hazard of new cases of active pulmonary tuberculosis with unconfirmed isolation of tubercle bacilli and patients registered in groups i (without isolation of tubercle bacilli) and ii of the dispensary observation was estimated in comparison to persons isolating tubercle bacilli. the hazard of the patients with active tuberculosis of the lungs and not confirmed isolation of tubercle bacilli for the members of their families and neighbours was shown to be 5-6 time ... | 1989 | 2516907 |
| [new drugs for tuberculosis: are they necessary in brazil?]. | | 1989 | 2516916 |
| [the antigenic and immunogenic substances in mycobacteria and the problem of creating a molecular vaccine]. | | 1989 | 2516948 |
| new strategies for leprosy and tuberculosis and for development of bacillus calmette-guérin into a multivaccine vehicle. | | 1989 | 2516995 |
| [studies on pyrazine derivatives. xxvi. synthesis and tuberculostatic activity of n-pyrazinylthiourea]. | 2-amino-3-chloropyrazine and 2-amino-6-chloropyrazine were reacted with appropriate sodium alkoxides to give 2-aminopyrazine derivatives with the methoxy, benzyloxy, chlorobenzyloxy, dichlorobenzyloxy, bromobenzyloxy or dibromobenzyloxy group at positions 3 and 6 (i-xiv). the obtained compounds were converted into n-pyrazinyl-n'-benzoylthioureas (xv-xxxi) by reacting with benzoyl isothiocyanate. their hydrolysis yielded n-pyrazinylthioureas xxxii-xlvii. analogical reactions of alkoxyaminopyrazin ... | 1989 | 2517187 |
| glycolipids of recent clinical isolates of mycobacterium tuberculosis: chemical characterization and immunoreactivity. | five distinct glycolipids were readily detected in isolates of mycobacterium tuberculosis. spectroscopic methods and chemical degradation techniques allowed the structural identification of four of these glycolipids. the specific phenolic glycolipid antigen previously characterized from the canetti strain was found in all the strains examined, with identical structural features (triglycosyl phenol phthiocerol dimycocerosate). the other three glycolipids identified were acylated trehaloses: penta ... | 1989 | 2517299 |