| glucose regulation of cephamycin biosynthesis in streptomyces lactamdurans is exerted on the formation of alpha-aminoadipyl-cysteinyl-valine and deacetoxycephalosporin c synthase. | glucose exerted a concentration-dependent negative regulation on the biosynthesis of cephamycin c by streptomyces lactamdurans. formation of the cephamycin precursor delta(alpha-aminoadipyl)-cysteinyl-valine was greatly decreased by excess glucose. the ring-expanding enzyme deacetoxycephalosporin c synthase was strongly repressed by glucose in vivo. isopenicillin n synthase (cyclase) and isopenicillin n epimerase were not repressed by glucose. however, the activity of isopenicillin n synthase wa ... | 1986 | 3794640 |
| suppression of free fatty acids and triglycerides in normal and hypertriglyceridemic rats by the adenosine receptor agonist phenylisopropyladenosine. | activation of adenosine receptors leads to the inhibition of lipolysis in the fat cells of several species including humans. we have examined the effects of the adenosine analog phenylisopropyladenosine (pia) on lipolysis in vivo in intact rats. pia suppresses markedly serum-free fatty acid (ffa) concentrations when injected s.c. (-)-pia is more potent than (+)-pia in suppressing ffa concentrations; also, this effect of (-)-pia is blocked by the adenosine receptor antagonist 8-(p-sulfophenyl)the ... | 1986 | 3795036 |
| inhibition of the platelet activating factor (paf)-induced in vivo responses in rats by trans-2,5-(3,4,5-trimethoxyphenyl) tetrahydrofuran (l-652,731), a paf receptor antagonist. | trans-2,5-bis-(3,4,5-trimethoxyphenyl)tetrahydrofuran (l652, 731), a potent and specific receptor antagonist of platelet activating factor (paf) (hwang et al., j. biol. chem. 260: 15639-15645, 1985), potently inhibits several paf-induced in vivo responses in rats. intravenously and p.o. administered l-652,731 gave a dose-response inhibition of paf-induced lysosomal hydrolase secretion and extravasation with ed50 values of 1 and 3 mg/kg, respectively. inhibitions of 87% were achieved with 50 mg/k ... | 1986 | 3795043 |
| [role of the diabetogenic factor in the activation of the blood coagulation system in experimental alloxan diabetes]. | | 1986 | 3797076 |
| response of dogs to challenge with ancylostoma ceylanicum during the tenure of a primary hookworm infection. | a model of human hookworm infection has been developed which shows that dogs currently infected with small numbers of hookworms are considerably resistant to a challenge infection with a large inoculum of infective larvae. two groups of dogs were infected with 500 larvae and four weeks after infection one group together with a previously uninfected control group were infected with 5,000 larvae and followed for six weeks. when compared with the secondary infection control dogs, faecal egg excreti ... | 1986 | 3798534 |
| rabbit endocervical epithelium: morphometric analysis of secretory cell populations. | in this report we quantitated ultrastructural changes in two cytologically distinct secretory cell populations from the rabbit endocervix. type i and type ii cells from estrous animals differ only in the presence of one or more empty cytoplasmic vacuoles in type ii cells. comparing type ii cells from 5-day pseudopregnant (psp) rabbits with type ii cells from estrous controls, there is no increase (p greater than .05) in the average vacuole volume. when type i and type ii cells from psp animals a ... | 1986 | 3799998 |
| [changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct]. | competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease. | 1986 | 3801148 |
| a contribution to indirect ophthalmotonometry in beagles. | a test of suitability of the electroimpression tonometer from fritz schwarzer gmbh, munich, for measuring intraocular pressure in dogs was performed. intraocular pressure of 63 clinically healthy english beagles was measured in both eyes using the instrument with different plunger weights. intraocular pressure and volume of corneal depression during measurement were determined as a function of the plunger weight from readings on the tonometer with the aid of friedenwald's tables (2). using fried ... | 1986 | 3807468 |
| inferior olive lesion induces long-term modifications of cerebellar inhibition on deiters nuclei. | the frequency of discharge of cerebellar purkinje cells and lateral vestibular nuclear cells were recorded at different intervals of time after injection of 3-acetylpyridine (3ap), which destroys the inferior olivary nucleus. during the first few days, purkinje cells showed an increase of simple spike firing, while deiters cells showed a strong depression of their discharge. recordings up to 3 months demonstrated, for both groups of cells, a recovery, whose time course is faster for deiters cell ... | 1986 | 3808481 |
| inferior olive lesion induces long-term modifications of cerebellar inhibition on deiters nuclei. | the frequency of discharge of cerebellar purkinje cells and lateral vestibular nuclear cells were recorded at different intervals of time after injection of 3-acetylpyridine (3ap), which destroys the inferior olivary nucleus. during the first few days, purkinje cells showed an increase of simple spike firing, while deiters cells showed a strong depression of their discharge. recordings up to 3 months demonstrated, for both groups of cells, a recovery, whose time course is faster for deiters cell ... | 1986 | 3808481 |
| the hypoxic ventilatory response of rats with increased blood oxygen affinity. | experimental work has established an interspecific relationship between the threshold for the hypoxic ventilatory response and the 'knee' of the hb-o2 dissociation curve. however, whether this relationship exists intraspecifically remains unclear. to examine the problem further rats were treated with sodium cyanate (naocn) to lower p50 and their hypoxic ventilatory response was measured. naocn treated rats had a lower pao2 and higher hct than control rats. there was no difference between the con ... | 1986 | 3809758 |
| the hypoxic ventilatory response of rats with increased blood oxygen affinity. | experimental work has established an interspecific relationship between the threshold for the hypoxic ventilatory response and the 'knee' of the hb-o2 dissociation curve. however, whether this relationship exists intraspecifically remains unclear. to examine the problem further rats were treated with sodium cyanate (naocn) to lower p50 and their hypoxic ventilatory response was measured. naocn treated rats had a lower pao2 and higher hct than control rats. there was no difference between the con ... | 1986 | 3809758 |
| nonproductive, cell-associated virus exists before the appearance of antiviral antibodies in experimental measles encephalitis. | the ability of measles virus to induce a cell-associated state of infection in the central nervous system (cns) plays an important role in the pathogenesis of subacute sclerosing panencephalitis (sspe), a fatal disease of children and young adults. the mechanism by which such an infection state is established is unknown, but several hypotheses have been proposed. one of these suggests that antiviral antibodies induce the nonproductive infection state by a process termed antigenic modulation. res ... | 1987 | 3811233 |
| furosemide lowers intracranial pressure by inhibiting csf production. | furosemide administration effectively lowers intracranial pressure in newborn preterm and term rabbit pups. this effect may be due to the diuretic action of the drug, its ability to inhibit cerebrospinal fluid production or to a combination of both. to test these possibilities newborn rabbits were either injected with furosemide and left unmolested for 6 hours, or anaesthetized and subjected to ventriculocisternal perfusions. during the 6 hour postnatal period the decrease in body weight was 8 t ... | 1986 | 3811613 |
| circulating forms of human foetal somatomedin. | two forms of somatomedin, which crossreact in a radioreceptor-assay using foetal brain membranes as matrix, have been partially purified from the serum of human foetuses aged 16-28 weeks of gestation. the purification scheme consisted of acid precipitation, sephadex g-50 chromatography, affinity chromatography and cation exchange fast protein liquid chromatography. the two peaks of activity had apparent molecular weights of approximately 7000 and different isoelectric points. the elution positio ... | 1987 | 3811771 |
| dose-titration of fenbendazole in the treatment of poultry nematodiasis. | broiler chickens were fed ad libitum diets containing fenbendazole at various concentrations and durations for the removal of adult ascaridia galli, heterakis gallinarum, and capillaria obsignata. all regimens were 100% effective against a. galli. fenbendazole at doses of 80 ppm for 3 days and 48 or 30 ppm for 5 days was 100% effective against h. gallinarum. c. obsignata was not removed completely by any regimen, but greater than or equal to 95% effectiveness was seen in birds given fenbendazole ... | 1986 | 3814008 |
| anticholinesterase properties of methamidophos and acephate in insects and mammals. | | 1987 | 3814841 |
| use of nude mouse xenografts as preclinical drug screens: in vivo activity of established chemotherapeutic agents against melanoma and ovarian carcinoma xenografts. | to evaluate the utility of nude mouse xenografts as preclinical drug screens, the activity of ten established chemotherapeutic agents was evaluated against seven melanoma and three ovarian carcinoma xenografts. xenografts were established using primary explants from patients who had not received chemotherapy and serially passaged as sc tumors in nude mice. in vivo drug activities for dactinomycin, carmustine, vinblastine, melphalan, amsacrine, cisplatin, bleomycin, mitomycin, doxorubicin, and et ... | 1987 | 3815395 |
| influence of low-dose beta-interferon on natural killer cell activity in breast cancer patients subjected to chemotherapy. | the present study was designed to test whether immunomodulating doses of human beta-interferon would affect the natural cell-mediated cytotoxic function in untreated breast cancer patients or in those subjected to antitumor therapy. analyses were performed on 11 breast cancer patients, 3 at stage 1 and 8 at stage 2, the latter being subjected to cyclophosphamide, methotrexate, 5-fluorouracil (cmf) adjuvant chemotherapy. five patients treated with cmf and 3 patients not subjected to adjuvant chem ... | 1987 | 3815421 |
| influence of low-dose beta-interferon on natural killer cell activity in breast cancer patients subjected to chemotherapy. | the present study was designed to test whether immunomodulating doses of human beta-interferon would affect the natural cell-mediated cytotoxic function in untreated breast cancer patients or in those subjected to antitumor therapy. analyses were performed on 11 breast cancer patients, 3 at stage 1 and 8 at stage 2, the latter being subjected to cyclophosphamide, methotrexate, 5-fluorouracil (cmf) adjuvant chemotherapy. five patients treated with cmf and 3 patients not subjected to adjuvant chem ... | 1987 | 3815421 |
| incidence of shigellosis and multi-drug resistant shigellae: a 10 year study. | between 1976 and 1985 a total of 12,709 faecal samples were cultured. from these samples 760 strains (6.0%) of shigellae were isolated. shigella flexneri was the commonest isolate (55%), followed by s. dysenteriae (29.7%). a total of 15.7% of children under 5 years of age were positive for shigellae. the rate of isolation was lower during the winter months than in the summer months. only 25 strains (3.3%) were sensitive to all five antibiotics tested. a total of 617 (81.2%) were resistant to thr ... | 1987 | 3820357 |
| reaction to injectable collagen: results in animal models and clinical use. | since its commercial release, zyderm collagen implant has been used to treat more than 200,000 subjects in the united states for soft-tissue contour defects and more than 250,000 patients internationally (including the united states). approximately 3 percent of subjects' skin tested with zyderm collagen experience localized hypersensitivity reactions to collagen, whereas approximately 1 percent of treated patients demonstrate symptoms of hypersensitivity at treatment sites. of the latter treatme ... | 1987 | 3823250 |
| phase shift of daily profiles of n-acetyltransferase in the rat pineal gland. | pineal n-acetyltransferase activity (nat) has a circadian rhythm with peak values in the dark time and low values in the light time. nat time profiles were measured in rats exposed to ld 14:10, to constant dark, and to acute (less than 48 hr) light-dark treatments. in all experiments, imposition of light suppressed nat. the phase of the dark time nat cycle was altered 2 hr or less by the following treatments: 3 hr light in the early subjective night, 3 hr light in the late subjective night, 2 hr ... | 1985 | 3831309 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | | 1985 | 3835674 |
| a new partition thin-layer chromatographic method for steroid separations. | tlc plates with a 25 mu thick polyamide stationary phase were modified for the separation of neutral steroids by impregnation with propylene glycol. a mixture of tritiated 5 alpha-androstan-3 alpha,17 beta-diol, testosterone, 17 beta-hydroxy-5 alpha-androstan-3-one and 4-androstene-3,17-dione was applied to the plate and developed in a toluene mobile phase to a height of 13.6 cm. this resulted in complete resolution of the 4 compounds as detected by a gas flow scanner or imaging analyzer. cuttin ... | 1985 | 3835732 |
| isolation and characterization of neuropeptide y from porcine intestine. | the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin. | 1985 | 3838090 |
| nucleotide sequences of the cdna and an intronless pseudogene for human lactate dehydrogenase-a isozyme. | eight cdna clones for lactate dehydrogenase-a isozyme (ldh-a) were isolated from a human fibroblast cdna library, characterized, and no sequence heterogeneity was found. four cdna clones appear to contain nearly full-length cdna inserts and the complete nucleotide sequence of 1710 base pairs consists of the protein-coding sequence (999 base pairs), the 5' (97 base pairs) and 3' (565 base pairs) untranslated regions and poly(da) tail (49 base pairs). the predicted amino acid sequence of the human ... | 1985 | 3838278 |
| molsidomine on cardiovascular leukotriene d4 actions. | anaesthetized open-chest dogs were used to study the effects of intracoronary leukotriene d4 (ltd4; 0.5 micrograms/kg) on haemodynamics, the electrocardiogram (ecg), coronary blood flow in the left circumflex artery and coronary resistance in the absence or presence of the antianginal drug molsidomine (500 micrograms/kg i.v.). ltd4 increased left ventricular (lv) filling pressure from 6.5 +/- 3.8 to 14.7 +/- 3.2 mm hg (p less than 0.01), decreased lv dp/dtmax from 2,500 +/- 200 to 1,240 +/- 205 ... | 1985 | 3839292 |
| myosin light chain phosphorylation and isometric twitch potentiation in intact human muscle. | the effects of a single voluntary contraction of the quadriceps muscle group on phosphate incorporation into the phosphorylatable light chains (p-light chains) of fast and slow myosin isolated from the vastus lateralis muscle and potentiation of the electrically stimulated twitch tension was studied in intact human muscle. twitch potentiation was maximal 20 s after the voluntary contraction. thereafter, twitch potentiation declined, but was still significantly higher than pre-contraction values ... | 1985 | 3839303 |
| heterogeneity of membrane phospholipid mobility in endothelial cells depends on cell substrate. | cellular growth control and differentiation have been shown to be dependent on both cell-cell and cell-substrate contacts. interactions of cells with extracellular material are critical events during embryonic development and maintenance of tissue function. plasma membrane receptors have been described for components of the extracellular matrix such as fibronectin, laminin and various collagen types. transmembrane signalling has been shown to be influenced by the lateral mobilities of the plasma ... | 1985 | 3839906 |
| 7-hydroxyguanine, a novel antimetabolite from a strain of streptomyces purpurascens. i. taxonomy of producing organism, fermentation, isolation and biological activity. | strain a-347, an actinomycete isolated from a soil sample, was found to produce a new antimetabolite, 7-hydroxyguanine. the aerial mycelium formed spiral spore chains with spiny spore surface. the chemical composition of strain a-347 indicated that it was an actinomycete of cell wall type i. from its morphological, cultural, physiological characteristics and direct comparison with the type culture, this strain was classified as streptomyces purpurascens. 7-hydroxyguanine was purified from the br ... | 1985 | 3840157 |
| isolation and hypoglycemic activity of ganoderans a and b, glycans of ganoderma lucidum fruit bodies. | | 1985 | 3840903 |
| tactile cutaneous representation in cerebellar granule cell layer of the opossum, didelphis virginiana. | recent studies of the albino rat revealed extensive cutaneous somatosensory projections to the granule cell (gc) layer of the cerebellar hemispheres and the caudal vermis. these projections are organized asomatotopically in patchy mosaics. to determine whether similar projections exist in a marsupial, we explored the gc layer of the cerebellar cortex of anesthetized virginia opossums using in-depth microelectrode micromapping and juxtathreshold cutaneous natural stimulation techniques. we found: ... | 1985 | 3843070 |
| powerline frequency electric and magnetic fields: a pilot study of risk perception. | the perception of the potential risk arising from human exposure to 50/60 hz electric and magnetic fields was studied with a quasi-random sample of 116 well-educated, opinion leaders using the risk perception framework previously developed by slovic, fischhoff, and lichtenstein. these individuals rated exposure to fields from transmission lines and electric blankets on a variety of scales that have been found useful in characterizing people's risk attitudes and perceptions. these judgments allow ... | 1985 | 3843677 |
| single-step purification of "kallikrein-resistant" kininogen from rat plasma using monoclonal-antibody immunoaffinity chromatography. | monoclonal antibody to rat plasma kininogen, obtained after immunization of mice with the kininogen prepared by conventional methods, was purified from ascites fluid and coupled to cnbr-activated sepharose-4b. monoclonal-antibody affinity adsorbant thus prepared provided a rapid single-step method of purifying to homogeneity plasma kininogen. purified rat plasma kininogen showed identical molecular weight and immunological cross-reactivity to rat plasma low molecular weight (lmw) kininogen purif ... | 1985 | 3851460 |
| recognition of a leukemia-related antigen by an antiidiotypic antiserum to an anti-gp70 monoclonal antibody. | the possibility that receptors for retroviral gp70 share structural elements with the antigen-binding sites of anti-retroviral gp70 antibodies was investigated. a monoclonal antibody (1416) was produced that reacted with the gp70 of a cloned recombinant leukemogenic retrovirus, termed p1. an antiidiotypic antiserum raised to 1416 was tested for its ability to bind to the thymic leukemia induced by p1 (p1 thy). a membrane structure was identified on the surface of p1 thy that reacted with the ant ... | 1985 | 3856626 |
| immigrants and a public oral health care service for children in denmark. | the present study was conducted to compare level of dental caries, treatment pattern and contact patterns with the child oral health care system of children of immigrants. the study comprised 239 turkish, 117 pakistani and 91 other immigrants and a control group of 359 danish children. data were extracted from the dental records in the clinic where they were treated. all groups of immigrants showed more caries in the primary dentition and less caries in the permanent dentition than danish childr ... | 1985 | 3860330 |
| cholecystokinin potentiation of dopamine-mediated behaviors in the nucleus accumbens. | | 1985 | 3861122 |
| [morphological changes in young rat mandible after release of muscle function during growth]. | | 1985 | 3864900 |
| protein loss of bovine dental enamel during in-vitro subsurface demineralization. | a chemical system based on the dialysis principle was used to study protein loss of dental enamel during demineralization with an acetic-acid buffer solution containing calcium and phosphate, in which the fluoride-ion activity was kept constant. this resulted in a subsurface lesion, with a depth of about 130 microns. after demineralization, protein material was isolated from the demineralization solution. u.v. spectra of the protein showed strong absorbance between 240 and 300 nm. amino-acid com ... | 1985 | 3865639 |
| [adaptation of rhodopseudomonas palustris to changes in atmospheric conditions]. | the high level of delta-aminolaevulinate synthetase (ala-s) in rhodopseudomonas palustris cells grown under photosynthetic conditions decreased when they were vigorously aerated; moreover, the bacteriochlorophyll synthesis suddenly stopped. when aeration was interrupted, enzymatic level increased notably, but bacteriochlorophyll level did not. chloramphenicol added to cultures when aeration was interrupted did not affect the increase of enzymic level. when it was added as the aeration started, t ... | 1985 | 3870744 |
| reactivities of neoplastic t cells to human autoantibody and monoclonal antibodies. | | 1985 | 3873778 |
| antifibrillatory actions of bepridil and butyl-mdi, two intracellular calcium antagonists. | the antifibrillatory and electrophysiologic actions of bepridil and butyl-methylenedioxyindene (bu-mdi), two intracellular calcium antagonists, were examined in anesthetized dogs. the administration of bepridil (1.0-10.0 mg/kg i.v.) significantly increased the electrical threshold for ventricular fibrillation determined during unobstructed coronary flow, and was associated with a significant decrease in ventricular excitability and a progressive depression in ventricular myocardial conduction. b ... | 1985 | 3874780 |
| isolation and characterization of epidermal growth factor from human milk. | epidermal growth factor (egf) has been purified from human milk. the purification was monitored with a human placental membrane radioreceptor assay using murine salivary epidermal growth factor i (megf i) as a competitive ligand and was achieved exclusively by the use of reverse-phase liquid chromatography (rplc). the sequential use of preparative, semipreparative and analytical rplc on an octylsilica support with solvent systems of different solute selectivity such as pyridine formate, triethyl ... | 1985 | 3874788 |
| role of the posterior capsule in the prevention of postoperative bacterial endophthalmitis: experimental primate studies and clinical implications. | the posterior capsule has an important effect on the risk of postoperative bacterial endophthalmitis. in order to investigate whether the posterior capsule inhibited the spread of infection into the vitreous we performed extracapsular cataract extraction in both eyes of 10 primates. in one eye of each primate the posterior capsule was left intact and in the other eye a large posterior capsulectomy was performed. when the anterior chambers were challenged with equivalent inocula of staphylococcus ... | 1985 | 3877524 |
| pathophysiology of the kidney in rats with heymann nephritis. | alterations in kidney function were assessed early in the course of heymann nephritis that was induced in rats by immunization with fx1a, an extract prepared from rat kidney cortex. whole kidney and single nephron function were evaluated by clearance and micropuncture techniques. kidney function was studied in stage 1 of heymann nephritis, before the onset of proteinuria, and in stage 2, when antibodies are deposited along the brush border of proximal tubules. although overall kidney function wa ... | 1985 | 3877841 |
| clinical studies with corticotropin releasing factor: implications for the diagnosis and pathophysiology of depression, cushing's disease, and adrenal insufficiency. | corticotropin releasing factor (crf) is a 41 amino acid peptide first isolated from sheep hypothalami and thought to be a principal modulator of the hypothalamic-pituitary-adrenal cortical (hpa) axis. we report herein a series of clinical studies with crf in healthy volunteers and in patients with abnormalities in hpa function, including depression, cushing's disease, cushing's syndrome, and addison's disease. our data indicate that crf can be a diagnostic aid in distinguishing various disorders ... | 1985 | 3878972 |
| aspartate: 2-oxoglutarate aminotransferase from trichomonas vaginalis. identity of aspartate aminotransferase and aromatic amino acid aminotransferase. | aspartate: 2-oxoglutarate aminotransferase from the anaerobic protozoon trichomonas vaginalis was purified to homogeneity and characterized. it is a dimeric protein of overall mr approx. 100000. only a single isoenzyme was found in t. vaginalis. the overall molecular and catalytic properties have features in common with both the vertebrate cytoplasmic and mitochondrial isoenzymes. the purified aspartate aminotransferase from t. vaginalis showed very high rates of activity with aromatic amino aci ... | 1985 | 3879173 |
| effect of octanoate on blood-brain barrier permeability using l-dopa as a marker. | short-chain fatty acid injection into animals produces coma, seizures, and hyperventilation. one mechanism of coma production may be through alterations in membrane permeability characteristics. the spg histofluorescence technique was used to evaluate changes in blood-brain barrier permeability to injected l-dopa in rats after intraperitoneal injections of the short-chain fatty acid, sodium octanoate. diffusion of intravascular fluorescence was observed around brain capillaries in the octanoate- ... | 1985 | 3880408 |
| effect of octanoate on blood-brain barrier permeability using l-dopa as a marker. | short-chain fatty acid injection into animals produces coma, seizures, and hyperventilation. one mechanism of coma production may be through alterations in membrane permeability characteristics. the spg histofluorescence technique was used to evaluate changes in blood-brain barrier permeability to injected l-dopa in rats after intraperitoneal injections of the short-chain fatty acid, sodium octanoate. diffusion of intravascular fluorescence was observed around brain capillaries in the octanoate- ... | 1985 | 3880408 |
| effect of antigen form on local immunoglobulin a memory response of intestinal secretions to shigella flexneri. | an enhanced memory response, as shown by increased titers of specific immunoglobulin a (iga), was seen in intestinal secretions from isolated thiry-vella loops in rabbits primed orally with live, locally invasive shigella sp. x16 and challenged 60 days later with a single oral dose of the same antigen. heat-killed shigella preparations, when used as either the priming or challenge antigen, did not elicit such a memory response in this system. in the present study, the role of antigen form and do ... | 1985 | 3880720 |
| an animal model of haemolytic--uraemic syndrome in shigellosis: lipopolysaccharides of shigella dysenteriae i and s. flexneri produce leucocyte-mediated renal cortical necrosis in rabbits. | to develop an animal model of the haemolytic-uraemic syndrome during shigellosis, rabbits were injected with lipopolysaccharides (lps) extracted by the hot phenol-water method from shigella dysenteriae i and from s. flexneri. two intravenous injections of lps spaced by 24 h elicited renal cortical necrosis in a generalized shwartzman reaction characterized by fibrin deposition in glomerular capillaries and by elevated plasma creatinine concentration. rabbits rendered leucopenic by busulphan trea ... | 1985 | 3882120 |
| vesicles and cisternae in the trans golgi apparatus of human fibroblasts are acidic compartments. | recently we demonstrated that low-ph compartments can be visualized with the electron microscope using a basic congener of dinitrophenol, 3-(2,4-dinitroanilino)-3'-amino-n-methyldipropylamine (damp), which concentrates in acidic compartments and can be detected by immunocytochemistry with a monoclonal anti-dinitrophenol antibody. we now report that damp also accumulates in cisternae and vesicles associated with the trans face of the golgi apparatus. damp rapidly leaves this compartment when cell ... | 1985 | 3882239 |
| evidence for proctolin-like substances in the central nervous system of the leech hirudo medicinalis. | the distribution of proctolin-like immunoreactive (pli) cells was mapped in the central nervous system of the leech hirudo medicinalis. in segmental ganglia, pli cells can be divided into two groups: cells that stain repeatedly in every successive ganglion, and cells that stain only in specific segmental ganglia. the number of pli cells, therefore, ranged from eight to 20 cells per ganglion. one bilateral pair of pli cells (cells pli-1) was further characterized morphologically by lucifer yellow ... | 1985 | 3882776 |
| fatal fungemia with arthritic involvement caused by trichosporon beigelii in a bone marrow transplant recipient. | | 1985 | 3882853 |
| rabbit antisera against three different bacteria which can induce reactive arthritis: analysis by elisa, immunoprecipitation and western blot. | three strains of bacteria which induce reactive arthritis were collected: a shigella flexneri, designated 7060; another sh. flexneri, designated 316; and a yersinia enterocolitica of serotype 03. rabbit antisera were generated against each of them to test for the extent and nature of cross-reactivity among these strains. when analysed by the elisa technique, antisera against 7060 and 316 showed strong cross-reactivity with y. enterocolitica. in contrast, the reaction of antisera prepared against ... | 1985 | 3884493 |
| plaque formation by virulent shigella flexneri. | an in vitro tissue culture plaque assay was developed to investigate the intracellular replication and intercellular spread of virulent shigellae. shigella plaques were formed in hela cell monolayers in the presence of an agarose overlay containing tissue culture medium and gentamicin, which eliminated extracellular bacterial growth. microscopically, the plaques were characterized by a central area of dead host cells surrounded by cells infected with shigellae. cells further away from the plaque ... | 1985 | 3884506 |
| cloning the gene for congo red binding in shigella flexneri. | the ability to bind the dye congo red from agar medium is associated with virulence of shigella species. dna sequences conferring this property have been cloned from a large, 140-kilobase plasmid of shigella flexneri into a plasmid vector. this recombinant plasmid does not fully restore virulence to s. flexneri isolates which have lost the large plasmid. this indicates that other genes present on the 140-kilobase plasmid must also be required for virulence of s. flexneri. the cloned fragment con ... | 1985 | 3884508 |
| shigella flexneri bacteraemia. | shigellosis is usually a non-invasive enteric disease, rarely accompanied by extra-intestinal manifestations. shigella septicaemia is therefore reported in a child aged 10 months. in the laboratory the organism was resistant to ampicillin and only moderately susceptible to chloramphenicol. the patient responded well to cotrimoxazole to which the organism isolated was susceptible in vitro. we recommend that blood as well as faeces should be cultured in exceptionally ill patients with suspected or ... | 1985 | 3884717 |
| effect of temperature on the circular dichroism spectra of beta 2-microglobulins. | when the temperature was lowered from 25 to 5 degrees c dramatic changes were observed in the near-ultraviolet circular dichroism spectra of bovine and caprine but not human beta 2-microglobulin. comparison of the protein sequences suggests that the conformational change occurs in the amino-terminal 24 residues and that a tyrosine residue located on a potential beta-turn acts as a reporter group. because delta h degrees is small (-22 kcal x mol-1), such conformational changes, possibly not readi ... | 1985 | 3886425 |
| studies of metabolites in diarrheal stool specimens containing shigella species by frequency-pulsed electron capture gas-liquid chromatography. | eleven diarrheal stool specimens and 10 control stool specimens from cairo, egypt, were studied by frequency-pulsed electron capture gas-liquid chromatography (fpec-glc). four cases involving shigella sonnei, three cases involving shigella boydii, and four cases involving shigella flexneri were studied. the aqueous stools were centrifuged, extracted with organic solvents, and derivatized to form specific electron-capturing derivatives of carboxylic acids, alcohols, hydroxy acids, and amines. ana ... | 1985 | 3886695 |
| perturbation of insulin oscillations by nerve blockade in the in vitro canine pancreas. | the in vitro canine pancreas produces an oscillatory pattern of insulin secretion during a constant glucose concentration despite the lack of external nervous modulation or recirculating hormone feedback. the normal period of insulin fluctuations (7.4 +/- 0.34 min) is unaffected by combined adrenergic and cholinergic blockade by 5 microm atropine, 4 microm propranolol, and 4 microm phentolamine (8.0 +/- 0.31 min, p less than 0.20). to test the theory that the coordination of islet secretion may ... | 1985 | 3887939 |
| erythema nodosum associated with shigella colitis. | | 1985 | 3888122 |
| survival of bacterial enteropathogens in the ice of popular drinks. | we examined the survival of four bacterial enteropathogens frozen in ice and subsequently allowed to melt in various popular drinks. the counts of all the organisms were markedly lowered by freezing alone, and the numbers were further decreased by exposure to some of the drinks. nevertheless, none of the organisms were completely eliminated as a result of freezing for 24 hours followed by melting in any of the test drinks, even when the drink was 86-proof tequila. | 1985 | 3889393 |
| [shigella serotypes isolated july-october 1983 and their antibiotic sensitivity]. | twenty four strains of shigella were isolated from the stool of patients who had been admitted to the hospital with the complaint of diarrhea. of these, 17 were found to be sh. flexneri, and 7 sh. boydii. antibiotic susceptibility tests indicated that all of the 24 isolates probably had multiple resistance factors. twenty three of 24 strains were found to be resistant to ampicillin. this may be important from a therapeutic point of view. | 1985 | 3892249 |
| [the shigella flexneri epidemic of 1983/84: the lesson to be learned from this]. | | 1985 | 3892308 |
| [antibiotic resistance in the isolates of shigella flexneri type 2 associated with the "shrimp epidemic"]. | | 1985 | 3892309 |
| [5 cases of reactive arthritis following a shigella flexneri epidemic in the netherlands]. | | 1985 | 3892310 |
| [bacillary dysentery in utrecht: 2 epidemics]. | | 1985 | 3892311 |
| [clinical observations during an epidemic caused by shigella flexneri]. | | 1985 | 3892312 |
| [detection of shigella antigens in the feces of patients with acute intestinal diseases using a coagglutination reaction]. | a total of 113 patients with acute intestinal diseases have been examined with the use of the coagglutination test. 84.95% of the patients showed the presence of different shigella antigens. in patients with bacteriologically confirmed dysentery the corresponding shigella antigens were detected in 96.97% of cases in s. sonnei dysentery, in 90% of cases in s. flexneri dysentery, in 75% of cases in s. newcastle dysentery and in 100% of bases in s. boydii dysentery. in 81.6% of patients with acute ... | 1985 | 3892986 |
| [possible production of 2 cytotoxins by a strain of escherichia coli]. | | 1985 | 3893462 |
| humoral response of the mouse to treponema pallidum. | to investigate the development of the humoral immune response of mice to infection with treponema pallidum, balb/c and c57bl mice were injected intradermally with 10 x 10(6) virulent organisms. serum samples were taken from the mice at weekly intervals after infection and used in the electrophoretic transblotting technique to detect t pallidum and t phagedenis biotype reiter antigens. the serum samples taken from infected mice at 21, 42, 84, and 126 days after infection recognised two to 15 t pa ... | 1985 | 3894212 |
| current diagnosis of infective endocarditis. | an analysis was made of 91 cases of infective endocarditis (ie) with regard to causative organisms and their sensitivities to various antibiotics, the clinical features of the disease, the laboratory test results and other items were important in establishing a diagnosis of ie. the number of cases of ie has shown a tendency to increase in recent years, particularly in the number of elderly patients, and the ratio of total cases consisting of prosthetic valve endocarditis (pve) has shown a sharp ... | 1985 | 3894710 |
| neonatal shigellosis with bowel perforation. | | 1985 | 3895181 |
| a marker of early amacrine cell development in rat retina. | a monoclonal antibody, hpc-1, labels only amacrine and displaced amacrine cells in adult rat retina. reactivity with displaced amacrine cells was demonstrated by double-label immunofluorescence with hpc-1 and ganglion cell-specific reagents. hpc-1 antibody reacts with a polypeptide of apparent molecular weight 35,000. hpc-1 antibody labels migrating amacrine cells in late embryonic retinas. the results define cell-specific gene expression in relation to migration of one subclass of cns neurons. | 1985 | 3896407 |
| [acute alithiasic cholecystitis caused by infectious gastroenteritis]. | | 1985 | 3897740 |
| [structural characteristics of the remote interaction of shigella and salmonella with enterocytes of experimental animals]. | the electron-microscopic analysis of the adhesion of shigella and salmonella nonfimbriated strains to the surface of enterocytes of laboratory animals in vivo and in vitro has revealed the presence of structural links, whose formation occurs with the participation of the glycocalix of the interacting cells and the extracellular gel. | 1985 | 3898674 |
| plasmid-mediated drug resistance of shigellae in kuwait. | of 153 clinical isolates of shigellae examined, 64.7% belonged to shigella flexneri, 18.9% to sh. sonnei, 11.8% to sh. boydii and 4.6% to sh. dysenteriae. part of these isolates were resistant to sulfamethoxazole and streptomycin (88.2% each), ampicillin (66.7%), tetracycline (63.4%) and co-trimoxazole (43.1%), with levels of resistance (mic50 and mic90) being invariably high. resistance to three or more drugs (multidrug resistance) was seen in 77.8% of the isolates. all the 25 strains examined ... | 1985 | 3899007 |
| [interrelation of erythropoiesis and lymphopoiesis in experimental infectious processes and in viral leukemogenesis]. | interrelation of erythropoiesis and immune response was studied in mice of various lines infected with mycoplasma species, shigella flexneri 2a and rauscher leukemia virus (r-mlv). intensity of erythropoiesis was estimated by the endocolonization data of sublethally irradiated mice and 59fe incorporation, while immune reactivity--by generation of splenic antibody-forming cells in response to immunization with sheep erythrocytes. the inverse correlation between the ability of infectious agents to ... | 1985 | 3899611 |
| four cases of shigella septicemia in israel. | four cases of shigella septicemia are presented. in two children infection was caused by shigella flexneri and in two adults, who had underlying diseases which caused immunosuppression, by shigella sonnei and shigella schmitzi. all four patients responded to intravenous antibiotic therapy. in one patient the organism persisted in the stool, but was eliminated after oral administration of chloramphenicol. shigella septicemia is not uncommon and blood cultures should be obtained in suspected cases ... | 1985 | 3899637 |
| hla-b27 and clinical aspects of ankylosing spondylitis: results of prospective studies. | | 1985 | 3899917 |
| shigellosis (antibiotic resistance and transfer of r-factor). | | 1985 | 3900357 |
| [functional status of monocytes of the peripheral blood in dysentery]. | | 1985 | 3900573 |
| comparative biology of intracellular parasitism. | | 1985 | 3900672 |
| [effectiveness of thymalin and prodigiozan in the complex treatment of patients with dysentery]. | | 1985 | 3901509 |
| sensitivity to bile salts of shigella flexneri sublethally heat stressed in buffer or broth. | batch cultures of shigella flexneri m4243 were grown at 37 degrees c in broth to early stationary phase, washed, and heated at 50 degrees c in 0.1 m phosphate buffer (ph 7.0). cells were surface plated on a tryptic phytone glucose agar (tpga), tpga with 0.15 or 0.85% bile salts no. 3 (tpga-bs 0.15 or tpga-bs 0.85), or tpga with 0.25 or 0.50% sodium deoxycholate (tpga-dc 0.25 or tpga-dc 0.50). cells sampled after no heating produced colony counts on tpga-bs 0.85 or on tpga-dc 0.50 that were no mo ... | 1985 | 3901919 |
| localization and synthesis of the tumor protein oncomodulin in extraembryonic tissues of the fetal rat. | the calcium-binding protein oncomodulin, previously found only in tumors, has been detected during rat development. specific antisera to purified rat hepatoma oncomodulin (mw 11,500) were used to detect oncomodulin by radioimmunoassay (ria) and by avidin-biotin-peroxidase complex (abc) immunohistochemistry. using ria, oncomodulin was found to increase in placenta from below the limits of detection (2 ng/mg protein) on day 13 to approximately 25 ng/mg on day 16 of pregnancy, and to remain high th ... | 1985 | 3902538 |
| susceptibility and hla-b27 in post-dysenteric arthropathies. | a recent outbreak of bacillary dysentery in the netherlands revealed that, despite the close association of hla-b27 with post-dysenteric or reactive arthritis (rea), not even in one family did all hla-b27 positive patients infected by an arthritogenic bacterium, develop rea. this dissociation shows that additional factors beside b27 may determine susceptibility to rea. | 1985 | 3902627 |
| cyclosporine and partial liver allotransplants in a simplified rat model. | we designed a simplified rat model to assess the effects of cyclosporine (csa) on liver transplants. auxiliary liver grafts consisting of 20 per cent of the whole liver were supplied with portal inflow and the bile was drained into the stomach. the cuff technique was used routinely for both end-to-end and end-to-side venous anastomoses. the host liver was totally deprived of portal inflow. two inbred strains of rats, lew and f344, were used for isograft (lew----lew) and allograft (f344----lew). ... | 1985 | 3903301 |
| [functional status of the small intestine in dysentery sonnei and flexneri]. | | 1985 | 3904028 |
| decreased chemiluminescent associated phagocytic response of peripheral blood mononuclear cells to chlamydia trachomatis in patients with hla-b27+ anterior uveitis. | the chemiluminescent (cl) associated phagocytic response of peripheral blood monocytes to two serovars of chlamydia trachomatis, shigella flexneri and zymosan was assessed in a group of 26 patients with anterior uveitis (au). hla-b27+ patients with au, when compared to hla-b27- patients with au and appropriate controls, had a significantly decreased cl response to c. trachomatis but no difference between groups in the response to s. flexneri and zymosan. the decreased chemiluminescent (phagocyti ... | 1985 | 3904888 |
| identification and antigenic characterization of virulence-associated, plasmid-coded proteins of shigella spp. and enteroinvasive escherichia coli. | seven plasmid-coded polypeptides, designated a through g, were identified by two-dimensional nonequilibrium ph gradient electrophoresis of radiolabeled extracts from minicells of virulent shigella flexneri serotypes 2a and 5 and enteroinvasive escherichia coli o143. these polypeptides were deemed to be products of 140-megadalton (mda) virulence-associated plasmids because they were not synthesized in minicells which were not harboring a 140-mda plasmid or in minicells which were carrying an f la ... | 1985 | 3905608 |
| characterization of monoclonal antibodies against shiga-like toxin from escherichia coli. | three monoclonal antibodies, designated mab 16e6, mab 13c4, and mab 19g8, were produced which recognize shiga-like toxin (slt) from escherichia coli. all three monoclonal antibodies neutralized the cytotoxicity of e. coli slt and were able to immunoprecipitate intact labeled toxin with staphylococcus aureus protein a. the three antibodies were of the g1 heavy and kappa light chain classes. mab 16e6 bound to the b subunit of slt in western blots and also neutralized the lethality of the toxin for ... | 1985 | 3905611 |
| interspecific reconstitution of maltose transport and chemotaxis in escherichia coli with maltose-binding protein from various enteric bacteria. | in escherichia coli, the periplasmic maltose-binding protein (mbp), the product of the male gene, is the primary recognition component of the transport system for maltose and maltodextrins. it is also the maltose chemoreceptor, in which capacity it interacts with the signal transducer tar (taxis to aspartate and some repellents). in studies of the maltose system in other members of the family enterobacteriaceae, we found that mbp is produced by salmonella typhimurium, klebsiella pneumoniae, ente ... | 1985 | 3905762 |
| changing pattern of resistant shiga bacillus (shigella dysenteriae type 1) and shigella flexneri in bangladesh. | shigella dysenteriae type 1 (shiga bacillus) has made a dramatic comeback in bangladesh after 10 years when shigella flexneri was the dominant serogroup. whereas s. flexneri showed little increase in resistance to the commonly used antimicrobial agents--namely, trimethoprim-sulfamethoxazole (tmp-smx) and ampicillin-over the years, s. dysenteriae type 1 underwent rapid changes with regard to sensitivity pattern during the last two years. the first tmp-smx-resistant strain of s. dysenteriae type 1 ... | 1985 | 3905980 |
| [3-year study of shigellosis epidemic in rwanda, central africa. problems of public health and bacteriological aspects]. | since 1979, the lakes region (kivu in zaïre, western uganda, rwanda, burundi) has been stricken by a bacillary dysentery epidemic at s. dysenteriae type 1. supposed to be extinct since the 1950s., this bacillus has revealed a very noticeable virulence as well by the number of registered cases (524/100.000 in 1983 at ruhengeri-rwanda) as its fast break through. demographic density (from 400 to 600/km2) has played an important role and has facilitated the transition toward epidemicity. systematic ... | 1985 | 3906345 |
| [the nature of the cyclic course of the epidemiological process in dysentery]. | | 1985 | 3907219 |
| determination of nanogram quantities of vanadium in biological material by isotope dilution thermal ionization mass spectrometry with ion counting detection. | | 1985 | 3907412 |
| refined structure of alkaline phosphatase from escherichia coli at 2.8 a resolution. | the structure of alkaline phosphatase from escherichia coli has been determined to 2.8 a resolution. the multiple isomorphous replacement electron density map of the dimer at 3.4 a was substantially improved by molecular symmetry averaging and solvent flattening. from these maps, polypeptide chains of the dimer were built using the published amino acid sequence. stereochemically restrained least-squares refinement of this model against native data, starting with 3.4 a data and extending in steps ... | 1985 | 3910843 |