| expression and nucleotide sequence of the clostridium acetobutylicum beta-galactosidase gene cloned in escherichia coli. | a gene library for clostridium acetobutylicum ncib 2951 was constructed in the broad-host-range cosmid plafr1, and cosmids containing the beta-galactosidase gene were isolated by direct selection for enzyme activity on x-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactoside) plates after conjugal transfer of the library to a lac deletion derivative of escherichia coli. analysis of various psup202 subclones of the lac cosmids on x-gal plates localized the beta-galactosidase gene to a 5.1-kb ecori fr ... | 1991 | 1850729 |
| catabolism of 3- and 4-hydroxyphenylacetic acid by klebsiella pneumoniae. | klebsiella pneumoniae catabolizes both 4-hydroxyphenylacetic acid and 3-hydroxyphenylacetic acid via meta-cleavage of 3,4-dihydroxyphenylacetic acid, ultimately yielding pyruvate and succinate. the organism can synthesize two hydroxylases catalysing 3,4-dihydroxyphenylacetic acid formation, which differ in substrate specificity, cofactor requirement, kinetics and regulation. five enzymes sequentially involved in the catabolism of 3,4-dihydroxyphenylacetic acid are encoded on a 7 kbp fragment of ... | 1991 | 1851804 |
| spread of klebsiella in a neonatal ward. | the colonization of infants with klebsiella pneumoniae was prospectively studied. samples were taken from nose, throat, umbilicus and rectum on the day of arrival and thereafter once a week. phage typing was performed the first time k. pneumoniae was found at any of these sites. settle plates were exposed in the incubators and in the patient rooms 5 h/day. the study lasted for 32 weeks. the first 15 weeks was a control period with no information to the staff, the following 4 weeks was a period o ... | 1991 | 1853167 |
| gentamicin-resistant klebsiella spp. and escherichia coli in a neonatal intensive care unit. | a case of septicemia in a 7-day-old infant with a gentamicin-resistant strain of klebsiella oxytoca prompted an epidemiological survey in a neonatal unit. within a 3-month period 7 patients presented with symptomatic infection with gentamicin-resistant gram-negative bacilli. another 3 patients were asymptomatic and only harboured the organisms in the stool. the aminoglycoside modifying enzyme ant(2")-a was identified with a probe technique in different gram-negative species indicating the spread ... | 1991 | 1853168 |
| gas-forming vertebral osteomyelitis in diabetic patients. | we report 2 cases of gas-forming vertebral osteomyelitis in diabetic patients. both were caused by gram-negative bacilli, escherichia coli and klebsiella pneumoniae respectively. both ran a fulminant course to death despite appropriate parenteral antibiotics. we suggest that early surgical intervention may be needed in cases with gas-forming vertebral osteomyelitis. | 1991 | 1853175 |
| high-level resistance to cefotaxime and ceftazidime in klebsiella pneumoniae isolates from cleveland, ohio. | two isolates of klebsiella pneumoniae possessing both tem-1 and shv-2 beta-lactamases were isolated from patients at the cleveland clinic in 1988. the beta-lactamases were discriminated and identified by using substrate hydrolysis data and an isoelectric focusing procedure in which the gel was overlaid with beta-lactamase inhibitors. | 1991 | 1854155 |
| bacteriology of the anal wound after open hemorrhoidectomy. qualitative and quantitative analysis. | the purpose of this study is to analyze the size of the bacterial colonies in anal wounds after open hemorrhoidectomy. twenty patients were studied during predetermined postoperative time periods. material was collected from the surface and from within the tissue of each patient's three open wounds, intraoperatively, on the 6th, 13th and 20th postoperative days for bacteriologic examination in aerobic, microaerophilic, and anaerobic media. the bacterium most commonly identified was escherichia c ... | 1991 | 1855423 |
| possible in-vivo transfer of beta-lactamase tem-3 from klebsiella pneumoniae to salmonella kedougou. | salmonella kedougou bm2659 was isolated from the stools and a blood culture of a patient and klebsiella pneumoniae bm2657 and s. kedougou bm2658 were isolated later from the stools of the same patient. strains bm2657 and bm2658 had identical resistance phenotypes, to beta-lactams, aminoglycosides and tetracycline, due to the presence of the same genes, blat, aaca4 and tetc, respectively. oligotyping indicating that beta-lactam resistance in these strains was encoded by blat-3 and synthesis of te ... | 1991 | 1856122 |
| concomitant dissemination of three extended-spectrum beta-lactamases among different enterobacteriaceae isolated in a french hospital. | from january 1988 to august 1989, 267 non-repetitive strains of enterobacteriaceae producing extended-spectrum beta-lactamases (esbla) derived from tem (ctx-1/tem-3, caz-6) or shv (caz-5) were isolated from 219 colonized or infected patients. esblas were characterized by analytical isoelectric focusing. biotypes, resistance phenotypes and plasmid patterns were determined in order to differentiate the isolates in each species. among the 116 ctx-1-producing enterobacteriaceae, 48 strains were diff ... | 1991 | 1856124 |
| antimicrobial effects of lomefloxacin in vitro. | the mic of lomefloxacin was determined for 554 isolates from the urinary tract. some of the more resistant strains of escherichia coli, proteus mirabilis, klebsiella pneumoniae, and pseudomonas aeruginosa were studied in a dynamic in-vitro model in order to study dosing strategies. the model simulated, in mueller-hinton broth, the profile of plasma lomefloxacin concentrations in volunteers. bacteria were exposed to a range of lomefloxacin concentration profiles achievable by oral dosing. the fir ... | 1991 | 1856127 |
| ankylosing spondylitis and klebsiella--the debate continues. | | 1991 | 1856800 |
| no conclusive evidence of an epidemiological relation between klebsiella and ankylosing spondylitis. | fifty-six patients with ankylosing spondylitis and 87 healthy controls were screened for klebsiella strains in their stools using a new highly sensitive culture medium. the presence of klebsiella strains in the patient group was compared with activity of the disease. in a dynamic study changes in klebsiella quantity over a period of 3 months were compared with changes in disease activity over the same period. the patient and control group showed similar percentages of klebsiella carriage. in the ... | 1991 | 1856808 |
| detection of klebsiella pneumoniae antibodies in aotus l. lemurinus (panamanian owl monkey) using an enzyme linked immunosorbent assay (elisa) test. | an enzyme linked immunosorbent assay (elisa), was adapted to detect antibodies against klebsiella pneumoniae in aotus l. lemurinus monkeys. it was used to define the prevalence of infection and the immunogenicity of an al(oh)3 bacterin in a population of laboratory born a. l. lemurinus monkeys. this represents a preliminary step to reduce k. pneumoniae produced mortality. a striking finding during a cross-sectional prevalence study was that none of the babies of less than 2 months old had detect ... | 1991 | 1857095 |
| characterization of the helicobacter pylori urease and purification of its subunits. | helicobacter pylori (formerly campylobacter pylori) is the causative agent of gastritis in man. helicobacter pylori cells contain a large amount of an extremely active urease (e.c.3.5.1.5). this enzyme is suspected to be a virulence factor since the ammonium ion produced from urea may be responsible for tissue injury and/or survival of h. pylori in the gastric environment. helicobacter pylori urease, native relative molecular mass approximately 600,000, was purified by agarose gel filtration and ... | 1991 | 1857197 |
| predictive value of oropharyngeal cultures for identifying lower airway bacteria in cystic fibrosis patients. | identifying lower respiratory pathogens in young, non expectorating cystic fibrosis (cf) patients has been problematic. bronchial secretions are difficult to obtain, and little is known about lower airway flora in these patients. we collected simultaneous bronchial and oropharyngeal specimens in 43 cf patients in optimal respiratory status, including both expectorating (17) and nonexpectorating (26) patients, to determine the predictive value of oropharyngeal cultures for identifying lower airwa ... | 1991 | 1859056 |
| analysis of virc, an operon involved in the secretion of yop proteins by yersinia enterocolitica. | upon incubation at 37 degrees c in the absence of ca2+ ions, pathogenic yersiniae release large amounts of pyv plasmid-encoded proteins called yops that are involved in pathogenesis. yersinia enterocolitica also expresses an outer membrane protein that is considered an adhesin and called yada (previously called p1 or yopa). the production of yops is coordinately regulated by a 20-kb region of the plasmid referred to as the ca2+ dependence region and containing at least four loci called vira, vir ... | 1991 | 1860816 |
| difficulties in identifying klebsiella strains of clinical origin. | two hundred and four strains of gram-negative bacteria of clinical origin, initially identified as klebsiella using the api 20 e system, and 10 reference strains were further analysed with the api 20 ec test system and the api 50 ch, api 50 ao, api 50 aa assimilation systems. four clusters corresponding to the species klebsiella pneumoniae subsp. pneumoniae, k. oxytoca, k. planticola, and k. terrigena were formed after numerical analysis of 155 selected tests and the 26 most discriminating tests ... | 1991 | 1863314 |
| antimicrobial activity of seven oral antibiotics against selected community- and hospital-acquired pathogens. | the in vitro activity of seven oral antibiotics (ciprofloxacin, cephalexin, cefaclor, cefadroxil, cefuroxime, cefixime, and amoxicillin/clavulanate) was tested against 355 hospital-acquired pathogens and 342 community-acquired organisms. whereas staphylococcus aureus and escherichia coli were the most common pathogens isolated in community-acquired infections, enterobacter, klebsiella, and pseudomonas were isolated most frequently in hospital-acquired infections. all antibiotics with the excepti ... | 1991 | 1863937 |
| epidemiology of beta-lactamases in africa: correlation with resistance to beta-lactam antibiotics. | in 45 centers from eight african countries, 2,888 bacterial isolates were collected from patients with community-acquired infections. isolated pathogens included staphylococcus aureus (29%), escherichia coli (20%), neisseria gonorrhoeae (6%), proteus species (6%), klebsiella species (6%), klebsiella pneumoniae (4%), and staphylococcus epidermidis (4%). an overall sensitivity of 16.2% was shown to penicillin g (number of isolates tested = 2,467), 31.8% to ampicillin (2,687), 45% to amoxicillin (1 ... | 1991 | 1863940 |
| effect of cytostatic agents on the number of alveolar phagocytes and the efficacy of ceftriaxone in an experimental murine lung infection. | mice made monocytopenic and granulocytopenic by cyclophosphamide or monocytopenic by etoposide were infected by exposure to an aerosol containing klebsiella pneumoniae. eighteen hours later ceftriaxone was administered and three hours after that the experiment was ended. at the time of infection and at 18 and 21 h the numbers of alveolar macrophages and granulocytes in bronchoalveolar lavage (bal) fluid were significantly lower in the cyclophosphamide-pretreated animals than in the controls. fur ... | 1991 | 1864349 |
| preliminary evaluation of biolog, a carbon source utilization method for bacterial identification. | the biolog identification system (biolog, inc., hayward, calif.) is a new bacterial identification method that establishes an identification based on the exchange of electrons generated during respiration, leading to a subsequent tetrazolium-based color change. this system tests the ability of a microorganism to oxidize a panel of 95 different carbon sources. we report on a preliminary investigation of the ability of the instrument to identify, using its computer-driven enzyme immunoassay reader ... | 1991 | 1864931 |
| antibodies to iron-regulated outer membrane proteins of coliform bacteria isolated from bovine intramammary infections. | expression of iron-regulated outer membrane proteins (omp) by escherichia coli and klebsiella pneumoniae initially isolated from bovine intramammary infections (imi) was investigated. additionally, the presence of antibodies in bovine serum and mammary secretion directed against the iron-regulated omp was examined. outer membrane proteins were separated by sodium-dodecyl polyacrylamide electrophoresis. detection of immunoglobulin g directed against omp was by immunoblotting. all gram-negative ba ... | 1991 | 1866891 |
| [reserves for lowering the neonatal mortality of premature children]. | a tendency for a decrease in the rate of neonatal mortality among preterm babies could be followed up in industrialized countries for the current decade. this tendency is less pronounced in the ussr. therefore newer diagnostic and therapeutic approaches developed in the clinical department of the scientific and research institute of pediatrics, the ussr academy of medical sciences, could be regarded as reserves for the reduction of neonatal mortality in preterm children. they include the analysi ... | 1991 | 1867001 |
| neonatal sepsis in pakistan. presentation and pathogens. | the case records of all neonates admitted to the neonatal unit at aga khan university hospital (karachi) in a 30 month period (nov. 86-april 89) were analysed. of 60 neonates with confirmed sepsis, 33 (55%) had non-nosocomial infection (nnc) whereas 27 (45%) had nosocomial sepsis (nc). the most common organisms causing early-onset nnc sepsis were klebsiella species (53%) and escherichia coli (10%), whereas the organisms causing late-onset nnc sepsis included salmonella parathypi (21%), group a s ... | 1991 | 1867074 |
| intestinal colonization with enterobacteriaceae in pakistani and swedish hospital-delivered infants. | rectal cultures from swedish and pakistani hospital-delivered newborn infants were analysed regarding the early acquisition of enterobacteria. swedish infants were delivered vaginally, pakistani infants were delivered either vaginally or by caesarean section. the swedish infants were all breast-fed, whereas breastfeeding was incomplete and often started late among the pakistani infants. both groups of pakistani infants were more rapidly colonized with enterobacteria than were the swedish infants ... | 1991 | 1867075 |
| clinical patterns and associated conditions in chronic uveitis. | we determined the relative frequencies of the different types of chronic uveitis, and the possible associated conditions, among 400 consecutive patients with chronic uveitis examined during the years 1982 through 1988. of the 400 patients, 183 (45.75%) had anterior uveitis, 98 (24.5%) ahd panuveitis, 61 (15.3%) had intermediate uveitis, and 58 (14.5%) had isolated posterior uveitis. only four of the 98 patients with panuveitis (4.1%) were considered idiopathic after a comprehensive examination, ... | 1991 | 1867298 |
| experience with biliary audit. | experience with an audit of surgery for gallstones is discussed. three hundred and forty-six patients were treated in a three and a half year period. there was no mortality. there was a duct exploration rate of 17%, an infection rate of 8.6%, and a re-operation rate for retained bile duct stone of 1.6%. bile was cultured from 278 gall-bladders and culture was positive in 66 cases (23.7%). the most common organisms grown were coliforms and streptococci. our antibiotic policy has changed as a resu ... | 1991 | 1867610 |
| intermittent urethral self-catheterisation: long term results (bacteriological evolution, continence, acceptance, complications). | forty six (9%) out of 520 patients from the years 1976 to 1982 who used intermittent urethral self-catheterisation (iusc) as the method of bladder drainage at the moment of discharge from the swiss paraplegic centre of basel were analysed during their rehabilitation period, on discharge, 3, 6 months later and again 5 years later regarding bacteriological changes and urinary tract infections, behaviour of some problem germs such as proteus, klebsiella and pseudomonas, sterile versus clean cathete ... | 1991 | 1870888 |
| [new pathogenetic aspects of ankylosing spondylitis]. | ankylosing spondylitis is one of the oldest diseases in humans; however, it is still one of the most fascinating and mysterious in human pathology. the unusual combination of both fundamental pathological processes: inflammation and ossification (which are mostly independent in respect to time and place) is unique. until 1973, ankylosing spondylitis did not attract much immunological research. after the detection of an association between hla b27 and the disease, clinical and immunological resea ... | 1991 | 1872044 |
| septic metastatic lesions of pyogenic liver abscess. their association with klebsiella pneumoniae bacteremia in diabetic patients. | septic metastatic endophthalmitis from klebsiella pneumoniae liver abscess, first reported in seven cases treated at the veterans general hospital, taipei, taiwan, between 1981 and 1985, was seen in six similar cases at the same hospital in the subsequent 2 years. we conducted a retrospective search for factors that might be associated with these complications of pyogenic liver abscess. a total of 23 cases with septic metastatic lesions from pyogenic liver abscess were found between 1981 and 198 ... | 1991 | 1872659 |
| nitrogenase of klebsiella pneumoniae. reversibility of the reductant-independent mgatp-cleavage reaction is shown by mgadp-catalysed phosphate/water oxygen exchange. | the steady-state kinetics of reductant-independent atp hydrolysis by klebsiella pneumoniae nitrogenase at 23 degrees c at ph 7.4 were determined as a function of component protein ratio (optimal at an oxidized fe protein/mofe protein ratio of 3:1) and mgatp concentration (km 400 microm). competitive inhibition was observed for mgadp (ki 145 microm), [beta gamma-methylene]atp (mgp[ch2]ppa) (ki 115 microm), [beta gamma-monofluoromethylene]atp (mgp[chf]ppa) (ki 53 microm) and [beta gamma-difluorome ... | 1991 | 1872810 |
| in vitro activity of an oxycephem ocp 9-176 compared with its sulfur analog and other beta-lactams. | ocp 9-176 is an oxycephem antibiotic that contains a 2-aminothiazolyl, carboxypropyl side chain at c-7 and a pyridinium thiomethyl group at c-3. ocp 9-176 was generally twofold less active than its sulfur-containing analog me 1228 against escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, providencia stuartii, proteus vulgaris, and serratia marcescens. activity against enterobacter cloacae and citrobacter freundii was equivalent. ocp 9-176 was twofold less active than me-1228 against p ... | 1991 | 1873972 |
| novel transferable extended-spectrum beta-lactamase (shv-6) from klebsiella pneumoniae conferring selective resistance to ceftazidime. | a clinical isolate of klebsiella pneumoniae sensu lato isolated from throat and a blood culture taken from a neutropenic patient treated for 2 weeks with ceftazidime and vancomycin was resistant to ceftazidime (mic: 32 micrograms/ml) and moderately susceptible to aztreonam (mic: 4 micrograms/ml). the isolate contained a plasmid of 180 kb which, when transferred to escherichia coli by conjugation, conferred resistance to ceftazidime and tetracycline. the transconjugant had decreased susceptibilit ... | 1991 | 1874404 |
| a rapid fluorogenic method for the detection of escherichia coli by the production of beta-glucuronidase. | a medium containing the fluorogenic substrate 4-methylumbelliferyl-beta-d-glucuronide was developed for the isolation and identification of escherichia coli within 7.5 h and was based on the detection of beta-glucuronidase. optimum conditions for the rapid development of fluorescent colonies were determined. the optimum temperature was 41.5 degrees c. development of fluorescence was delayed when carbohydrates were incorporated into the medium. water samples were used to evaluate the medium by su ... | 1991 | 1874684 |
| intravenous ciprofloxacin for the treatment of severe infections. | intravenous ciprofloxacin at a daily dosage of 400 mg divided in two doses was administered to 19 patients with severe infections caused by ciprofloxacin-susceptible bacteria. these infections included: 11 surgical would infections, 5 soft tissue infections, 2 respiratory tract infections, 1 urinary tract infection. the offending pathogens were: 8 coagulase-negative staphylococci, 3 staphylococcus aureus, 3 pseudomonas aeruginosa, 2 proteus spp., 1 escherichia coli, 1 branhamella catarrhalis, 1 ... | 1991 | 1875231 |
| [endophthalmitis in japan--a nationwide study with reference to type and etiology]. | a nationwide clinical study on bacterial and fungal endophthalmitis was done with special reference to their type and etiology. two hundred and eighty cases of endophthalmitis were reviewed. all cases were divided into two categories such as exogenous (trauma, surgery, and ulcer) and endogenous (metastasis). penetrating trauma was responsible for 93 cases (33.2%), surgery for 88 cases (31.4%), ulcer for 11 cases (3.9%) and metastasis for 88 cases (31.4%). out of the 280 cases, 79 cases (28.2%) w ... | 1991 | 1877411 |
| cys184 and cys187 of nifl protein of klebsiella pneumoniae are not absolutely required for inhibition of nifa activity. | the sequence cys184-ala-asp-cys187 in the nifl protein of klebsiella pneumoniae, for which a role in oxygen sensing and/or metal binding has been proposed, was altered by introducing two mutations, cys184----ala and cys187----ala, using oligodeoxyribonucleotide-directed mutagenesis. neither mutation abolished ammonium or oxygen control of nif transcription, although some impairment of function was apparent. the two cys residues are therefore unlikely to have a direct role in oxygen sensing or me ... | 1991 | 1879702 |
| comparative study of amoxycillin and amoxycillin/clavulanic acid in lower respiratory infections. | a comparison of oral amoxycillin (500 mg tds) with amoxycillin/clavulanic acid (augmentin; 750 mg tds) for 7 to 10 days was completed in 76 patients with lower respiratory infection. in another 9 patients, intravenous augmentin alone was administered (1.2 g 8 hourly) for 3 days followed by oral doses as above for 7 days. in 50 (59%) patients the underlying chronic lung disease was bronchiectasis. clinical improvement (1 + or more) was seen in 66% with amoxycillin, 60% with oral augmentin and 56% ... | 1991 | 1880092 |
| klebsiella pneumonia with lung abscess. | | 1991 | 1880107 |
| n-acetyl-beta-d-glucosaminidase, etiologic agent, and duration of clinical signs for sequential episodes of chronic clinical mastitis in dairy cows. | this study examined effects of repeated episodes of clinical mastitis in chronically infected quarters on milk n-acetyl-beta-d-glucosaminidase activity and duration of clinical signs. milk samples were collected at each clinical onset from 49 chronic mastitis cases on a 1700-cow michigan dairy farm. there were 49 first episodes of clinical mastitis, 49 second episodes, and 13 episodes of third or more. agents isolated were staphylococcus aureus (18.4%), staphylococcus (7.3%), no growth (20.2%), ... | 1991 | 1880262 |
| organization and function of binding sites for the transcriptional activator nifa in the klebsiella pneumoniae nife and nifu promoters. | the interaction of the klebsiella pneumoniae nifa protein, a sigma 54-dependent activator, with the nife and nifu promoters was analysed. at these promoters nifa established contacts in addition to those predicted by the minimal formulation nifa binding site (5'-tgt-n10-aca). the positions of the contacts indicate that bound nifa molecules could assemble to form an oligomer. at both promoters contacts with nifa are made predominantly on one face of the dna helix, and all contacts appear necessar ... | 1991 | 1880804 |
| [bacteriological and clinical studies of cefodizime in pediatrics]. | bacteriological and clinical studies on cefodizime (cdzm, thr-221), a new cephem developed by hoechst ag and roussel uclaf, were carried out and the results are summarized below: 1. against gram-positive bacteria, staphylococcus aureus, streptococcus pyogenes and streptococcus pneumoniae, antibacterial activities of cdzm were similar to those of cefotaxime (ctx), cefazolin, cefotiam and piperacillin. against escherichia coli, klebsiella pneumoniae and serratia sp., antibacterial activities of cd ... | 1991 | 1880919 |
| [bacteriological, pharmacokinetic and clinical studies of cefpirome in the field of pediatrics]. | bacteriological, pharmacokinetic and clinical studies on cefpirome (cpr, hr 810), a new cephem antibiotic, were carried out in the field of pediatrics. the results obtained are summarized below. 1. antibacterial activities of cpr against clinically isolated strains of staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, streptococcus agalactiae, escherichia coli, klebsiella pneumoniae, haemophilus influenzae and haemophilus parainfluenzae were superior to those of ceftazidime ... | 1991 | 1880922 |
| [clinical evaluation of cefpirome in children]. | cefpirome (cpr, hr 810) was clinically evaluated for its efficacy and safety in 11 patients with ages from 4 months to 11 years with bacterial infection. the results obtained are summarized as follows. 1. cpr was administered to 6 patients with bronchopneumonia, a patient with pneumonia, a patient with tonsillitis, 2 patients with acute pharyngitis and a patient with suppurative parotitis at daily dosage levels ranging 55.5-91.7 mg/kg, divided into 3 using intravenous bolus injection or 30 minut ... | 1991 | 1880923 |
| [combined antibacterial activity of aztreonam and clindamycin against clinically isolated strains]. | it has been reported in some studies that the combination of aztreonam (azt) and clindamycin (cldm) have high clinical effectiveness in the treatment of intractable infections. we, therefore, studied combined in vitro antibacterial activity of these 2 compounds using many freshly isolated strains. 1. azt and cldm in combination had synergistic effects on staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, and haemophilus influenzae, which are sensitive or quasi-sensitive ... | 1991 | 1880932 |
| [in vitro sensitivity of gram-negative bacteria isolated from urinary tract infections to various antibiotics]. | this study was done on the outpatients and hospitalized patients who were suffering from urinary tract infections in türkiye yüksek ihtisas hospital. 500 positive cultures were evaluated. e. coli was the predominant organism and it is followed by klebsiella and coliform bacteria. the sensitivity of the isolated bacteria were tested against various antibiotic discs using disc diffusion method. the sensitivity of the bacteria to the used antibiotics were as follows: ceftazidime 96%, ofloxacin 95.6 ... | 1991 | 1881356 |
| [comparative activities of 15 beta-lactam antibiotics against 590 strains of klebsiella pneumoniae according to the production of beta-lactamase]. | in october 1988, all non repetitive strains of k. pneumoniae isolated in 17 hospitals have been studied. among these 590 strains: 451 (76%) only produce the specific beta-lactamase of the species shv-1 (pi 7,7) or shv-1 type (pi 7,1), while 74 (12.5%) produce a tem-1 or tem-2 type beta-lactamase, and 65 (11%) an extended broad spectrum beta-lactamase: 22 ctx-1, 5 shv-2, 4 shv-3, 26 shv-4, 8 shv-5. the minimum inhibitory concentrations of the following antibiotics were performed by a liquid micro ... | 1991 | 1881669 |
| [enterobacteriaceae isolated in nine general hospitals of the ile-de-france in 1988 and 1989: susceptibility to beta-lactam antibiotics according to the length of hospitalisation]. | susceptibility to beta-lactam antibiotics of strains of enterobacteriaceae consecutively isolated in nine general hospitals during a period of 2 months (march and april) has been studied by the disk-agar diffusion method. the separation between susceptible and resistant strains was based on the measure of the inhibition zones centered by 2 disks: cephalothin and ticarcillin. enterobacteriaceae were divided in 2 groups: strains isolated during the first 48 h of hospitalisation or isolated after. ... | 1991 | 1881679 |
| [pharmacokinetics and tissue penetration of single-dose netilmicin used for antibiotic prophylaxis during colo-rectal surgery]. | pharmacokinetics and tissue penetration of netilmicin were studied after the use of a single dose (6 mg/kg) given for antibioprophylaxis in colo-rectal surgery. thirteen patients, scheduled for elective surgery, were given 6 mg/kg iv netilmicin over 30 min, together with 1000 mg iv ornidazole. netilmicin peak serum concentration (10 min after end of infusion) was 24.4 +/- 3.4 mg/l and trough level (24 h) was 0.9 +/- 0.5 mg/l. plasma elimination half-life was 409 +/- 70 min, le volume apparent vo ... | 1991 | 1881684 |
| [the specific activity of a multicomponent vaccine made from the antigens of opportunistic microorganisms]. | multicomponent vaccine prepared from the antigens of 4 representatives of opportunistic microflora possesses high specific activity. the passive hemagglutination (pha) test with the use of associated diagnosticum showed that antibody titers in the sera of immunized rabbits increased 10- to 10(4)-fold in comparison with the titers observed prior to immunization. the pha test with the use of the antigens contained in the vaccine revealed the accumulation of antibodies to each of the 4 components o ... | 1991 | 1882605 |
| [the efficacy of klebsiella pneumoniae bacteriophage in the therapy of experimental klebsiella infection]. | the effectiveness of specific phage therapy was studied on klebsiella experimental sepsis in noninbred white mice, caused by the intraperitoneal injection of k. pneumoniae highly virulent strain k2 5055 into the animals. for treatment, klebsiella polyvalent bacteriophage administered on day 2 after the infection of the animals with klebsiella was used. the study revealed that bacteriophage could be detected in the blood and internal organs of the animals within 24 hours irrespective of the route ... | 1991 | 1882608 |
| [the immunomorphological characteristics of the appendix in a viral-bacterial lesion in children with appendicitis]. | the characteristics of viral-bacterial lesion in the appendix of children with appendicitis are given by means of histologic, histochemical, immunomorphological and morphometric methods. antigens of influenza viruses a, b, c, entero-, adeno- and paramyxoviruses were observed among which influenza c was found significantly more frequently (64.1%). viral infection in the majority of cases (79.5%) was found in the association with opportunistic flora, more frequently with e. coli, and less r. aerug ... | 1991 | 1883273 |
| postsepsis bradycardia in children with leukemia. | we observed sinus bradycardia in a small number of children with hematologic malignancies who were recovering from sepsis. our objective was to define this symptom complex and attempt to delineate its etiology. | 1991 | 1884617 |
| the anaerobic utilisation of cyanide in the presence of sugars by microbial cultures can involve an abiotic process. | the degradation of cyanide under anaerobic conditions in the presence of a growing culture of a strain of klebsiella planticola has been shown to be due to a chemical process dependent upon the presence of a reducing sugar in the medium. the conversion of cyanide to ammonia was independent of any biological factors under these conditions. | 1991 | 1884979 |
| nucleotide sequence and genetic analysis of the azotobacter chroococcum nifusvwzm gene cluster, including a new gene (nifp) which encodes a serine acetyltransferase. | nucleotide sequence was obtained for a region of 7,099 bp spanning the nifu, nifs, nifv, nifw, nifz, and nifm genes from azotobacter chroococcum. chromosomal mutations constructed at several sites within the locus confirmed a requirement for this region for expression of the molybdenum nitrogenase in this organism. the genes are tightly clustered and ordered as in klebsiella pneumoniae except for two additional open reading frames (orfs) between nifv and nifw. the arrangement of genes in a. chro ... | 1991 | 1885524 |
| false-positive chlamydiazyme results during urine sediment analysis due to bacterial urinary tract infections. | our study examined whether urinary tract infections (utis) would cause false-positive results when urine sediment was tested with the chlamydiazyme (cz) system. thirty-six infected urine samples and fifteen controls were studied. all controls were negative. forty-seven percent of escherichia coli utis (n = 30) and 100% of klebsiella pneumoniae utis (n = 4) were positive on cz testing of urine sediment. nine e. coli utis positive by cz were negative by direct fluorescent-antibody staining. when s ... | 1991 | 1885739 |
| trabulsiella guamensis, a new genus and species of the family enterobacteriaceae that resembles salmonella subgroups 4 and 5. | in 1985 the vernacular name enteric group 90 was coined for a small group of strains that had been referred to our laboratory as probable strains of salmonella but did not agglutinate in salmonella typing antisera. by dna-dna hybridization (hydroxyapatite method, 32p), seven strains of enteric group 90 were found to be closely related (98 to 100% at 60 degrees c and 94 to 100% at 75 degrees c) to the first strain received (0370-85). the relatedness of enteric group 90 to 62 strains of other spec ... | 1991 | 1885744 |
| [susceptibilities of bacteria isolated from patients with respiratory infectious diseases to antibiotics (1988)]. | isolated bacteria from respiratory infectious diseases were collected in cooperation with institutions located throughout japan, since 1981, and ikemoto et al. have been examining sensitivities of the isolates to various antibacterial agents and antibiotics, relationships between the isolates and the backgrounds of the patients and so forth each year. we report here the research results for the year 1988. in 18 institutions around the entire japan from october 1988 to september 1989, 554 strains ... | 1991 | 1886202 |
| [the peripheral joint syndrome in ankylosing spondylitis--one of the variants of reactive arthritis]. | in 75 patients with central (n = 37) and peripheral (n = 38) ankylosing spondylarthritis, eia was used to detect serum antibodies to klebsiella (igg and iga) and to the common enterobacterial antigen (ceba) as compared to the level of the esr, c-reactive protein and circulating immune complexes. out of the 75 patients, 53 were examined for the intestinal microflora. serum antibodies to klebsiella were demonstrated more frequently in the peripheral form than in the central one, particularly in de ... | 1991 | 1887423 |
| [immune disorders and sensitization to microbial antigens in seronegative spondylitis]. | a study was made of immunity in seronegative spondylarthritis. in ankylosing spondylarthritis, reactive arthritides and psoriatic arthritis, the decrease of t cells, t helpers and t suppressors was identified. patients with ankylosing spondylarthritis and reactive arthritides manifested an increase of the b-lymphocyte count. patients with ankylosing spondylarthritis were demonstrated to be sensitive to klebsiella (52%), those with reactive arthritides to intestinal yersinia (44%). the phenotype ... | 1991 | 1887424 |
| [the mutagenicity of a new multicomponent vaccine made from the antigens of klebsiella pneumoniae, staphylococcus aureus, proteus vulgaris and escherichia coli]. | inactivated bacterial vaccine, containing k. pneumoniae, s. aureus, p. vulgaris and e. coli antigenic complexes were tested for mutagenicity in the test described by ames et al. and in vivo, in experiments on mice. in salmonella typhimurium cells, strain ta-98 and ta-100, the preparation (5-75 mg/ml) did not increase the frequency of reversions and histidine-independence either in direct experiments or after metabolic activation with rat liver homogenate. in experiments on mice the vaccine (3.3 ... | 1991 | 1887711 |
| the differential effect of cigarette smoke on the growth of bacteria found in humans. | the effect of cigarette smoke on growth of those species of bacteria that are considered common potential human pathogens was examined in vitro. smoke from both mentholated and nonmentholated cigarettes inhibited the growth of gram-positive cocci to a greater degree than that of gram-negative rods. staphylococcus aureus, streptococcus pneumoniae, and a variety of other streptococci were inhibited at a smoke solution dilution of 1:8. enteric bacteria such as klebsiella, enterobacter, and pseudomo ... | 1991 | 1889244 |
| treatment of a meningitis due to an enterobacter aerogenes producing a derepressed cephalosporinase and a klebsiella pneumoniae producing an extended-spectrum beta-lactamase. | a case of nosocomial meningitis due to a klebsiella pneumoniae producing a caz-5 extended-spectrum beta-lactamase and an enterobacter aerogenes producing a derepressed cephalosporinase is reported. the intrathecal catheter incriminated was removed and a treatment with ceftazidime (4 g/24 h) and amikacin (1.5 g/24 h) was started. after 24 h ceftazidime was replaced by imipenem (2 then 4 g/24 h). this treatment failed to obtain cerebrospinal fluid sterilization; therefore the imipenem dosage was i ... | 1991 | 1889874 |
| combination effect of sce-2787 and cefepime with aminoglycosides on nosocomial gram-negative bacteria. | the in vitro activity of cefepime and sce-2787, two new parenteral cephalosporins, and the combination effect with tobramycin and gentamicin against nosocomial gram-negative rods was studied using checkerboard agar dilution technique. cefepime showed excellent in-vitro activity against klebsiella pneumoniae, enterobacter cloacae, serratia marcescens and proteus vulgaris (mic90 0.03-0.125 mg/l) and good to moderate activity against acinetobacter anitratus, pseudomonas aeruginosa and pseudomonas c ... | 1991 | 1889877 |
| the importance of positive bacterial cultures of specimens obtained during clean orthopaedic operations. | microbiological cultures of specimens of tissue and of fluids from the wound in forty patients who had had consecutive clean, elective orthopaedic operations (excluding total joint replacements) and had not received antibiotics preoperatively were analyzed. of the forty patients, twenty-three (58 per cent) had a positive culture on at least one of the media that were used and seventeen (43 per cent) had negative cultures. of the forty specimens that were obtained from swabbing of the wound, eigh ... | 1991 | 1890121 |
| ciprofloxacin in neonates and its possible adverse effect on the teeth. | | 1991 | 1891292 |
| inhibition of acid secretion from parietal cells by non-human-infecting helicobacter species: a factor in colonization of gastric mucosa? | helicobacter pylori has been shown to produce a protein that inhibits acid secretion from parietal cells. we have examined other non-human-infecting helicobacter species for this property by measuring the uptake of [14c]aminopyrine into rabbit parietal cells as an indirect assessment of acid secretion. helicobacter felis and an isolate from a rhesus monkey were shown to inhibit acid secretion. isolates of helicobacter mustelae gave variable responses. whole bacteria and cell-free sonicates impai ... | 1991 | 1894369 |
| structural and functional analysis of the rcsa gene from erwinia stewartii. | rcsa is a positive regulator of genes for extracellular polysaccharide biosynthesis in the enterobacteriaceae. the nucleotide sequence of the rcsa gene from erwinia stewartii was determined and compared to rcsa sequences from e. amylovora, escherichia coli, and klebsiella pneumoniae. three highly conserved regions of the gene were identified. the c-terminal end of the open reading frame (orf) shared significant amino acid homology to the luxr class of bacterial activator proteins. insertion and ... | 1991 | 1896018 |
| protein secretion in pseudomonas aeruginosa: the xcpa gene encodes an integral inner membrane protein homologous to klebsiella pneumoniae secretion function protein pulo. | xcp mutations have pleiotropic effects on the secretion of proteins in pseudomonas aeruginosa pao. the nucleotide sequence of a 1.2-kb dna fragment that complements the xcp-1 mutation has been determined. sequence analysis shows the xcpa gene product to be a 31.8-kda polypeptide, with a highly hydrophobic character. this is consistent with a localization in the cytoplasmic membrane in p. aeruginosa, determined after specific expression of the xcpa gene under control of the t7 phi 10 promoter. a ... | 1991 | 1898929 |
| rhythmic bacterial susceptibility to antibiotics at a large hospital. | the in vitro susceptibility response of staphylococcus aureus, klebsiella pneumoniae, escherichia coli, proteus mirabilis and pseudomonas aeruginosa to a set of antibiotics was investigated in a survey comprising 19,380 positive cultures over a period of 5 years in a large hospital environment. four out of the five species (p. aeruginosa being the exception) presented a species-specific, drug-independent, rhythmic variation of their level of susceptibility to several antibiotics over the time of ... | 1991 | 1899881 |
| spontaneous contrast in the pericardial sac caused by gas-forming organisms. | purulent pericarditis is a life-threatening disease associated with a variety of microorganisms. the case presented herein has unusual manifestations of contrast echoes on two-dimensional echocardiography. to our knowledge, this is the first reported case of pericarditis caused by gas-forming group d streptococcus and klebsiella pneumoniae with pyopericardium, resulting in contrast echoes on the two-dimensional echocardiographic image. | 1991 | 1900698 |
| periodic acid-schiff-positive organisms in primary cutaneous bacillus cereus infection. case report and an investigation of the periodic acid-schiff staining properties of bacteria. | primary cutaneous bacillus cereus infection frequently presents as a single necrotic bulla on the extremity of an immunocompromised patient. in lesional biopsy specimens and smears, the large gram-positive rods of b cereus may be mistaken for clostridium species. this is a potentially serious error, as bacillus species are resistant to penicillin and other beta-lactam antibiotics. we studied a case in which large periodic acid-schiff-staining organisms were seen in the biopsy specimen from a nec ... | 1991 | 1900984 |
| [ru 51807 (cefpodoxime proxetil). in vitro and in vivo antibacterial activity of a new orally administered active cephalosporin]. | cefpodoxime proxetil (ru 51807) is an enterally absorbed ester prodrug which is rapidly cleaved in vivo after oral administration, with release of the active free acid metabolite cefpodoxime. the in vitro antibacterial activity of the sodium salt of cefpodoxime (ru 51746) against approximately 800 clinical isolates was evaluated comparatively with other orally active beta-lactams. ru 51746 was found to be active against enterobacteria normally susceptible to third generation cephalosporins, with ... | 1991 | 1901403 |
| in vitro and in vivo activities of ljc10,627, a new carbapenem with stability to dehydropeptidase i. | the activity of ljc10,627 was compared with the activities of imipenem and other antibiotics. ljc10,627 was more active against most members of the family enterobacteriaceae, pseudomonas spp., and acinetobacter spp. but slightly less active than imipenem against staphylococci and streptococci. ljc10,627 showed stability to mouse dehydropeptidase i and was more effective in vivo than imipenem plus cilastatin against gram-negative bacterial infections and as effective against staphylococcal infect ... | 1991 | 1901698 |
| viability and distribution of bacteria after passage through a circle anaesthetic system. | we have assessed in vitro the viability of eight species of micro-organism suspended as aerosols and passed through a soda-lime absorber rebreathing system. as had been predicted, the soda-lime exerted a potent cidal effect on non-sporing organisms, all of which were rendered non-viable. one percent of the spore bearing organism, bacillus subtilis, was still viable after 30 min contact. although bacillus subtilis is an organism of low pathogenicity, spores may be more resistant to the alkaline m ... | 1991 | 1901723 |
| analysis of the lacz sequences from two streptococcus thermophilus strains: comparison with the escherichia coli and lactobacillus bulgaricus beta-galactosidase sequences. | the lacz gene from streptococcus thermophilus a054, a commercial yogurt strain, was cloned on a 7.2 kb psti fragment in escherichia coli and compared with the previously cloned lacz gene from s. thermophilus atcc 19258. using the dideoxy chain termination method, the dna sequences of both lacz structural genes were determined and found to be 3071 bp in length. when the two sequences were more closely analysed, 21 nucleotide differences were detected, of which only nine resulted in amino acid cha ... | 1991 | 1901904 |
| further observations on enhancement of the infectivity of fusobacterium necrophorum by other bacteria. | it had already been shown with a single virulent strain (a42) of fusobacterium necrophorum that suspension of the fusobacteria in sub-lethal doses of broth cultures of other bacteria reduced the minimum infective dose (greater than 10(6) organisms) for mice by subcutaneous inoculation, sometimes to less than 10 organisms. the present study extended the known range of bacteria with strong infectivity-enhancing properties to include bacillus cereus, klebsiella oxytoca and staphylococcus aureus; an ... | 1991 | 1902184 |
| expression of regulatory nif genes in rhodobacter capsulatus. | translational fusions of the escherichia coli lacz gene to rhodobacter capsulatus nif genes were constructed in order to determine the regulatory circuit of nif gene expression in r. capsulatus, a free-living photosynthetic diazotroph. the expression of nifh, nifa (copies i and ii), and nifr4 was measured in different regulatory mutant strains under different physiological conditions. the expression of nifh and nifr4 (the analog of ntra in klebsiella pneumoniae) depends on the nifr1/r2 system (t ... | 1991 | 1902215 |
| production and characterization of a human hyperimmune intravenous immunoglobulin against pseudomonas aeruginosa and klebsiella species. | a hyperimmune globulin for intravenous use (h-ivig) was prepared from the plasma of donors immunized with pseudomonas aeruginosa and klebsiella vaccines. h-ivig preparations contained substantially higher igg antibody levels to all nine p. aeruginosa vaccine antigens and to 22 of the 24 klebsiella vaccine antigens than did commercial ivig. the h-ivig was more effective at promoting the opsonophagocytic killing of p. aeruginosa and klebsiella vaccine serotype strains than normal ivig. the h-ivig ... | 1991 | 1902245 |
| bacteriological and protozoal study on domestic water supply in karachi. | four hundred and fifty eight water samples collected from domestic tanks and taps and community taps were analysed for bacterial contamination. faecal pollution was more in domestic tanks and taps in mehmoodabad, korangi and least in water collected from community taps of liaquatabad, korangi and mehmoodabad. bacteria isolated were mostly e. coli, enterobacter sp, klebsiella sp. and a. faecalis, while other organisms (except pseudomonas) were found in lower numbers. salmonella para typhi and par ... | 1991 | 1902529 |
| purification and properties of a chromosomal beta-lactamase from klebsiella oxytoca. | a beta-lactamase was purified from klebsiella oxytoca strain gn10650. the enzyme was chromosomally-mediated and gave a single protein band on polyacrylamide gel electrophoresis. its pi was 5.34 and its mw was approximately 27,000. the optimal ph and temperature were about 7.0 and 50 degrees c, respectively. the specific activity of the enzyme was 1,207 units per mg of protein for hydrolysis of penicillins and cephalosporins, including cefuroxime, cefotaxime, and aztreonam. the enzyme activity wa ... | 1991 | 1903375 |
| cloning of nitrogenase structural genes from the obligate methanotroph methylococcus capsulatus (bath). | southern hybridization techniques were used to examine the dna homologies between the three nitrogenase structural genes nifh, nifd and nifk of klebsiella pneumoniae and dna from the obligate methane oxidizing bacterium methylococcus capsulatus (bath). the high degree of homology between methanotroph dna sequences and the klebsiella nifh and nifd genes was used to isolate and clone the corresponding methylococcus nifh and nifd genes. subsequent restriction analysis revealed that all three nif st ... | 1991 | 1904041 |
| identification and characterization of two entry exclusion genes of the promiscuous incp plasmid r18. | two entry exclusion genes (designated eexa and eexb) from the promiscuous incp alpha plasmid r18 have been isolated by molecular cloning. they are located between coordinates 26.6-27.4 kb and 27.4-27.6 kb, respectively and are transcribed clockwise on the conventional r18 map. the product of the eexa gene has an apparent molecular mass of 28 kda and its n-terminus contains a putative signal sequence for protein export. a recombinant plasmid containing r18 eex genes exerted eex activity towards a ... | 1991 | 1904533 |
| in vitro and in vivo evaluation of ro 09-1428, a new parenteral cephalosporin with high antipseudomonal activity. | ro 09-1428, a new parenteral cephalosporin with a catechol moiety attached at position 7 of the cephalosporin ring, showed high in vitro activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, and streptococcus pyogenes, with mics for 90% of strains tested (mic90s) of less than or equal to 0.39 micrograms/ml. morganella morganii, providencia rettgeri, citrobacter freundii, haemophilus influenzae, staphylococcus aureus, and streptococcus pneumoniae were inhi ... | 1991 | 1906261 |
| microbial aspects of failing osseointegrated dental implants in humans. | the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ... | 1991 | 1906537 |
| community acquired pneumonia: aetiology and prognostic index evaluation. | a 10 month prospective study of all adults admitted to waikato hospital with community acquired pneumonia was performed to assess aetiology, mortality, hospital stay, and the value of a prognostic index based on that obtained from a british thoracic society study. the 92 patients in the survey had a mean age of 56 (range 13-97) years. a microbiological diagnosis was established in 72%; streptococcus pneumoniae (33%), mycoplasma pneumoniae (18%), and influenza a virus (8%) were the most common mi ... | 1991 | 1907034 |
| antigenic homology among gram-negative organisms isolated from cattle with clinical mastitis. | this study examined the degree of serologic homology among mastitis pathogens. antibodies were raised against the rc mutant, escherichia coli o111:b4 (strain j5) and affinity purified against lipopolysaccharide derived from the ra mutant, salmonella typhimurium tv119. these antibodies reacted with a battery of unrelated gram-negative bacteria in whole cell elisa. bacteria with strong cross-reactions included a heterologous, smooth e. coli, salmonella dublin, s. typhimurium, salmonella newport, a ... | 1991 | 1907303 |
| effect of sub-minimal inhibitory concentrations of ciprofloxacin and fleroxacin on the bacterial capsular antigen and opsonophagocytosis by human polymorphonuclear leukocytes. | we have studied the effect of sub-minimal inhibitory concentrations (sub-mic) of ciprofloxacin and fleroxacin on capsulated (k+) and non-capsulated (k-) gram-negative bacilli (escherichia coli o1:k1, o7:k1, o1:k-, o7:k-, and klebsiella oxytoca) as well as on staphylococcus aureus and we investigated the interaction of antibiotic pretreated bacteria with human serum and polymorphonuclear leukocytes (pmn). following overnight growth in the presence of 1/2 mic of the antibiotics, bacteria were opso ... | 1991 | 1907453 |
| selective removal of molybdenum traces from growth media of n2-fixing bacteria. | a new method for the selective removal of traces of molybdenum from growth media of n2-fixing bacteria (rhodobacter capsulatus and klebsiella pneumoniae) was developed. this method is based on the filtration of nutrient solutions through a layer of activated carbon (pulverized charcoal). the adsorption of mo (molybdate) to activated carbon was optimal if a charcoal suspension (50 g/liter) was degassed by boiling before use and if the ph of the solutions, which had to be purified, was adjusted to ... | 1991 | 1908197 |
| activity of some antiseptics against urinary tract pathogens growing as biofilms on silicone surfaces. | the activity of chlorhexidine, mandelic acid and a mandelic/lactic mixture were tested against biofilms of four species of gram-negative nosocomial organisms that commonly infect the catheterized urinary tract. cells growing on silicone discs were exposed to concentrations of agents used in bladder instillation. citrobacter diversus biofilms proved to be sensitive to all three agents. pseudomonas aeruginosa, proteus mirabilis and klebsiella pneumoniae all survived well in chlorhexidine but their ... | 1991 | 1908381 |
| [diagnosis and therapy of pneumonia caused by gram-negative bacillus including legionella]. | | 1991 | 1908504 |
| survey on use of antimicrobial agents and bacterial resistance in huashan hospital. | a cross-section study was carried out to assess the general patterns in use of antimicrobial agents and the trends of bacterial resistance in huashan hospital. of 2,400 patients whose charts were reviewed, 61% were given such drugs. 3,596 antibiotic courses were prescribed. gentamicin was most frequently used. results of the susceptibility test of 320 bacterial strains showed a high percentage of resistance against gentamicin, ampicillin, and chloramphenicol. our findings suggest that antibiotic ... | 1991 | 1908763 |
| physical protection against airborne pathogens and pollutants by a novel animal isolator in a level 3 containment laboratory. | a containment laboratory unit for research with aerosols of group 2 pathogenic microorganisms is described. the design criteria are based on current uk guidelines, which imply containment at group 3 level during aerosol production, storage, exposure of animals and sampling. within the aerosol laboratory, primary containment is provided by a henderson apparatus operating at a negative pressure to the external environment. flexible film isolators under negative pressure are used for all hazardous ... | 1991 | 1908783 |
| [nosocomial infections in an urological department. incidence and etiological factors]. | the authors report a prospective study with active request of data for nosocomial infections (ni) in an urological department during six months. from 453 patients, 43 developed an ni (incidence = 9.5%): urinary tract infections (53.5%), bloodstream infections (16.3%), lower respiratory tract infections (7%), surgical wound infections (2.3%) and sepsis syndrome (20.9%). for microorganisms, most often pseudomonas aeruginosa (22.5%) and e. coli (20%) were encountered. other microorganisms were the ... | 1991 | 1909021 |
| formaldehyde-resistance in enterobacteriaceae and pseudomonas aeruginosa: identification of resistance genes by dna-hybridization. | a 4.1. kb large dna fragment of a e. coli plasmid pvu 3695, on which the genes for formaldehyde-resistance are located, was used as a dna probe to identify bacteria that carry this segment among formaldehyde-resistant bacteria. it was shown by southern blot-, dot blot-, and colony blot- hybridization studies that the dna of all formaldehyde-resistant e. coli, serratia marcescens, enterobacter cloacae, citrobacter freundii and klebsiella pneumoniae strains tested hybridize with the dna probe from ... | 1991 | 1909132 |
| cefotaxime-hydrolysing activity of the beta-lactamase of klebsiella oxytoca d488 could be related to a threonine residue at position 140. | the chromosomally encoded beta-lactamase of klebsiella oxytoca d483 strain, active against all third-generation cephalosporins but ceftazidime, was purified to homogeneity. the pure protein was digested by trypsin, staphylococcus aureus v8 protease or proteinase asp-n. amino acid sequences of the hplc-separated proteolytic peptides were determined by manual edman degradation. overlapping fragments gave the alignment of the 263 residues of the beta-lactamase which presented 90% homology with the ... | 1991 | 1909282 |
| immunogenicity and protective efficacy of pertussis toxin subunit s1 produced by bacillus subtilis. | pertussis toxin (pt) subunit s1 was produced in bacillus subtilis as a secretory protein designated bacs1. bacs1 was partially purified and used to immunize mice. the sera were tested for pt-neutralizing antibodies and for protective capacity in a mouse model. unlike previous findings with recombinant s1 from escherichia coli, the recombinant bacs1 protein induced antibodies that were both neutralizing and protective. an adjuvant was necessary for efficient immunization with bacs1 but not with p ... | 1991 | 1909767 |
| microbiology of chronic sinusitis in children. | to better understand the factors involved in chronic sinusitis in childhood, we cultured the sinuses, middle meatus, and nasopharynx in 39 children requiring surgical intervention. sixty-nine percent of these patients had other medical problems, including asthma (49%) and immunologic compromise (18%). we cultured coagulase-negative staphylococcus in 18 patients, streptococcus viridans in 14 patients, normal flora in 10 patients, staphylococcus aureus in nine patients, group d streptococcus in fi ... | 1991 | 1910729 |
| late perinephric abscess formation after extracorporeal shock wave lithotripsy. | | 1991 | 1913081 |
| influence of environmental conditions on infection of klebsiella pneumoniae by two different types of bacteriophages. | the adsorption and efficiency of plating of bacteriophages fc3-1 and fc3-9 on klebsiella pneumoniae c3 (serotype o1:k66) cells grown at different phs and temperatures were quantitated. bacteriophage fc3-1, with lipopolysaccharide as its bacterial receptor, showed a large decrease in efficiency of plating on bacteria grown at low ph or low temperature. under the same conditions, no significant decrease in efficiency of plating was found for bacteriophage fc3-9, a phage requiring capsule and lipop ... | 1991 | 1913339 |