| metabolism of alcaligenes denitrificans in biofilm vs planktonic cells. | to compare the effect of phosphorous concentration (200 mg p l(-1) and 20 mg p l(-1)) on the denitrifying efficiency of alcaligenes denitrificans when in the form of planktonic cells or in the form of a biofilm, and to select the most adequate c/n ratio. | 2002 | 11849353 |
| evaluation of the id 32e for the identification of gram-negative glucose-fermenting and glucose-non-fermenting bacilli. | objective: to evaluate the id 32e bacterial identification system for accuracy in the identification of members of the family enterobacteriaceae, pseudomonas aeruginosa, stenotrophomonas maltophilia, and acinetobacter baumannii/iwoffii. methods: stock cultures of 497 enterobacteriaceae and 27 commonly encountered non-enteric gram-negative rods were tested in the id 32e system. for each isolate, the resulting 11-digit profile number was converted to an identification using the apilab plus softwar ... | 1999 | 11856267 |
| postkeratoplasty endophthalmitis by alcaligenes faecalis: a case report. | to describe a postkeratoplasty endophthalmitis owing to alcaligenes faecalis that resolved with medical management. | 2002 | 11862104 |
| side-chain effect of second monomer units on crystalline morphology, thermal properties, and enzymatic degradability for random copolyesters of (r)-3-hydroxybutyric acid with (r)-3-hydroxyalkanoic acids. | three types of random copolymers with 94 mol % (r)-3-hydroxybutyric acid (3hb) and 6 mol % (r)-3-hydroxyalkanoic acids with different side-chain lengths, (r)-3-hydroxypentanoic acid (3hv), (r)-3-hydroxyhexanoic acid (3hhx), and medium-chain-length (r)-3-hydroxyalkanoic acids (mcl-3ha, c8-c12), were prepared by biological synthetic techniques. the solid-state structure and thermal properties of melt-crystallized films for copolymers were characterized by means of wide-angle x-ray diffraction, sma ... | 2002 | 11866565 |
| the comparative in vitro activity of fk-037 (cefoselis), a new broad-spectrum cephalosporin. | fk-037, a new parenteral cephalosporin, was active against clinical isolates of both gram-positive and gram-negative aerobic bacteria. the activity of fk---037 was similar to that of cefpirome and cefepime and generally superior to that of ceftazidime. methicillin-resistant staphylococci were less susceptible than methicillin-sensitive isolates but most remained within the sensitive range. all streptococci and enterobacteriaceae were sensitive as were the majority of isolates of acinetobacter sp ... | 1995 | 11866715 |
| alcaligenes xylosoxidans dissimilatory nitrite reductase: alanine substitution of the surface-exposed histidine 139l ligand of the type 1 copper center prevents electron transfer to the catalytic center. | nitrite reductase of alcaligenes xylosoxidans contains three blue type 1 copper centers with a function in electron transfer and three catalytic type 2 copper centers. the mutation h139a, in which the solvent-exposed histidine ligand of the type 1 copper ion was changed to alanine, resulted in the formation of a colorless protein containing 4.4 cu atoms per trimer. the enzyme was inactive with reduced azurin as the electron donor, and in contrast to the wild-type enzyme, no epr features assignab ... | 2002 | 11876652 |
| characterization of an extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase from pseudomonas alcaligenes lb19. | an extracellular medium-chain-length poly(3-hydroxyalkanoate) (mcl-pha) depolymerase from an isolate, pseudomonas alcaligenes lb19, was purified to electrophoretic homogeneity by hydrophobic interaction chromatography using octyl-sepharose cl-4b and gel permeation chromatography using sephadex g-150. the molecular mass of the enzyme, which consisted of a single polypeptide chain, was approximately 27.6 kda. the pi value of the enzyme was estimated to be 5.7, and its maximum activity was observed ... | 2002 | 11888314 |
| nonhydrolytic fragmentation of a poly[(r)-3-hydroxybutyrate] single crystal revealed by use of a mutant of polyhydroxybutyrate depolymerase. | this paper reports the initial process of the enzymatic degradation of solution-grown lamellar single crystals of bacterial poly[(r)-3-hydroxybutyrate] (p(3hb)) with an extracellular polyhydroxybutyrate (phb) depolymerase purified from alcaligenes faecalis t1. we used a hydrolytic-activity-disrupted mutant of the phb depolymerase in order to avoid the influence of hydrolytic reaction in the system. the effect of addition of the mutant enzyme upon the p(3hb) single crystals was investigated by tu ... | 2002 | 11888317 |
| synergistic activities of macrolide antibiotics against pseudomonas aeruginosa, burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans isolated from patients with cystic fibrosis. | azithromycin and clarithromycin were paired with other antibiotics to test synergistic activity against 300 multidrug-resistant pathogens isolated from cystic fibrosis (cf) patients. clarithromycin-tobramycin was most active against pseudomonas aeruginosa and inhibited 58% of strains. azithromycin-trimethoprim-sulfamethoxazole, azithromycin-ceftazidime, and azithromycin-doxycycline or azithromycin-trimethoprim-sulfamethoxazole inhibited 40, 20, and 22% of stenotrophomonas maltophilia, burkholder ... | 2002 | 11897598 |
| halogenated metabolites from the new okinawan red alga laurencia yonaguniensis. | a novel brominated diterpene based on the rare neoirieane skeleton, named neoirietetraol (1), has been isolated along with a halogenated c15 acetogenin, (3z)-laurenyne (2), from a new laurencia species, l.yonaguniensis masuda et abe, species inedita, collected at yonaguni island, okinawa prefecture, japan. the structures of these metabolites were elucidated by spectroscopic data (ir, 1h nmr, 13c nmr, 2d nmr, and ms). neoirietetraol (1) was toxic to the brine shrimp (altemia salina; lc50, 40.1 mi ... | 2002 | 11908989 |
| [nonlinearity in the growth of bacterial colonies: conditions and causes]. | the universally recognized kinetic model of colony growth, introduced by pirt, predicts a linear increase of colony size. the linearity follows from the assumption that the colony expands through the growth of only such cells that are located immediately behind the moving colony front, in the so-called peripheral zone of constant width and density. in this work, pirt's model was tested on two bacteria--alcaligenes sp. and pseudomonas fluorescens--having markedly distinct cultural properties and ... | 2002 | 11910809 |
| purification and characterization of alkaline protease from alcaligenes faecalis. | extracellular alkaline protease from the alkalophilic bacterium alcaligenes faecalis was purified by a combination of ion-exchange and size-exclusion chromatographic methods, and its properties were examined. the purified enzyme had a specific activity of 563.8 micromol of tyrosine/min per mg of protein and gave a single band on native page and sds/page with a molecular mass of 67 kda. gelatin zymogram also revealed one clear zone of proteolytic activity which corresponded to the band obtained w ... | 2002 | 11916457 |
| ribosomal dna-directed pcr for identification of achromobacter (alcaligenes) xylosoxidans recovered from sputum samples from cystic fibrosis patients. | the opportunistic human pathogen achromobacter (alcaligenes) xylosoxidans has been recovered with increasing frequency from respiratory tract culture of persons with cystic fibrosis (cf). however, confusion of this species with other closely related respiratory pathogens has limited studies to better elucidate its epidemiology, natural history, and pathogenic role in cf. misidentification of a. xylosoxidans as burkholderia cepacia complex is especially problematic and presents a challenge to eff ... | 2002 | 11923333 |
| [a case of liver abscess with particular pathological features due to alcaligenes xylosoxidans infection]. | | 2002 | 11925869 |
| the hydrogen-sensing apparatus in ralstonia eutropha. | molecular hydrogen is widely used by microorganisms as a source of energy. one of the best studied aerobic hydrogen oxidizers, the beta-proteobacterium ralstonia eutropha (formerly alcaligenes eutrophus), harbors two distinct [nife]-hydrogenases which catalyze the heterolytic cleavage of h2 into 2h+ and 2e-. the genes encoding the hydrogenase subunits are arranged in two large operons together with accessory and regulatory genes involved in hydrogenase biosynthesis. both operons are transcribed ... | 2002 | 11931556 |
| transformation of thiodiglycol by resting cells of alcaligenes xylosoxydans pgh10. | a new strain of alcaligenes xylosoxydans able to aerobically cometabolize thiodiglycol, the primary hydrolysis product of sulfur mustard, was isolated and tested in a laboratory scale stirred tank reactor. the strain, named pgh10, cannot use tdg as sole carbon and energy source for growth, but resting cells previously grown on either rich broth or defined mineral media efficiently metabolize this compound through [(2-hydroxyethyl)thio]acetic acid and thiodiacetic acid as intermediates. degradati ... | 2002 | 11934292 |
| investigation of an outbreak due to alcaligenes xylosoxydans subspecies xylosoxydans by random amplified polymorphic dna analysis. | in 1999, over a 3-week period, alcaligenes xylosoxydans subsp. xylosoxydans was isolated from five blood cultures and one cerebrospinal fluid specimen from five children hospitalized in a pediatric hematology ward as well as from two respiratory therapy devices of two children hospitalized in an intensive care unit. the infection control unit of the hospital conducted an epidemiological investigation and identified a detergent-disinfectant solution as the source of contamination. conventional bi ... | 2002 | 11939390 |
| the substrate specificity of l-asparaginase from alcaligenes eutrophus. | | 1971 | 11945731 |
| novel carbohydrate-binding module of beta-1,3-xylanase from a marine bacterium, alcaligenes sp. strain xy-234. | a beta-1,3-xylanase gene (txya) from a marine bacterium, alcaligenes sp. strain xy-234, has been cloned and sequenced. txya consists of a 1,410-bp open reading frame that encodes 469 amino acid residues with a calculated molecular mass of 52,256 da. the domain structure of the beta-1,3-xylanase (txya) consists of a signal peptide of 22 amino acid residues, followed by a catalytic domain which belongs to family 26 of the glycosyl hydrolases, a linker region with one array of dgg and six repeats o ... | 2002 | 11948152 |
| the x-ray structure of ferric escherichia coli flavohemoglobin reveals an unexpected geometry of the distal heme pocket. | the x-ray structure of ferric unliganded lipid-free escherichia coli flavohemoglobin has been solved to a resolution of 2.2 a and refined to an r-factor of 19%. the overall fold is similar to that of ferrous lipid-bound alcaligenes eutrophus flavohemoglobin with the notable exception of the e helix positioning within the globin domain and a rotation of the nad binding module with respect to the fad-binding domain accompanied by a substantial rearrangement of the c-terminal region. an inspection ... | 2002 | 11964402 |
| comparative evaluation of the bd phoenix and vitek 2 automated instruments for identification of isolates of the burkholderia cepacia complex. | we evaluated two new automated identification systems, the bd phoenix (becton dickinson) and the vitek 2 (biomérieux), for identification of isolates of the burkholderia cepacia complex (bcc). the test sample included 42 isolates of the highly virulent and epidemic genomovar iii, 45 isolates of b. multivorans, and 47 isolates of other members of the bcc. rates of correct identification by the bd phoenix and vitek 2 were similar when all bcc isolates were considered (50 and 53%, respectively) but ... | 2002 | 11980954 |
| response of spring rape (brassica napus var. oleifera l.) to inoculation with plant growth promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase depends on nutrient status of the plant. | responses of rape (brassica napus var. oleifera l.) to inoculation with plant growth promoting rhizobacteria, pseudomonas putida am2, pseudomonas putida bm3, alcaligenes xylosoxidans cm4, and pseudomonas sp. dp2, containing 1-aminocyclopropane-l-carboxylate (acc) deaminase were studied using growth pouch and soil cultures. in growth pouch culture, the bacteria significantly increased root elongation of phosphorus-sufficient seedlings, whereas root elongation of phosphorus-deficient seedlings was ... | 2002 | 11989762 |
| plasmid-encoded asp operon confers a proton motive metabolic cycle catalyzed by an aspartate-alanine exchange reaction. | tetragenococcus halophila d10 catalyzes the decarboxylation of l-aspartate with nearly stoichiometric release of l-alanine and co(2). this trait is encoded on a 25-kb plasmid, pd1. we found in this plasmid a putative asp operon consisting of two genes, which we designated aspd and aspt, encoding an l-aspartate-beta-decarboxylase (aspd) and an aspartate-alanine antiporter (aspt), respectively, and determined the nucleotide sequences. the sequence analysis revealed that the genes of the asp operon ... | 2002 | 12003930 |
| effect of (1-->3)- and (1-->4)-linkages of fully sulfated polysaccharides on their anticoagulant activity. | chemically fully sulfated polysaccharides including xylan (-->4xylbeta-(1-->4)xylbeta1-->), amylose (-->4glcalpha-(1-->4)glcalpha1-->), cellulose (-->4glcbeta-(1-->4)glcbeta1-->), curdlan (-->3glcbeta-(1-->3)glcbeta1-->) and galactan (-->3galbeta-(1-->3)galbeta1-->), which have been isolated from korean clam, were prepared, and their anticoagulant activity was investigated. the results strongly suggest that the activity might not be depending on anomeric configuration (alpha or beta) or monosacc ... | 2002 | 12007475 |
| in vitro antiseptic susceptibility of clinical isolates from nosocomial infections. | to evaluate the susceptibility of a large number of strains to various antiseptics, we elaborated a simple, qualitative broth turbidity method in which we could quickly judge the efficacy visually. for this method, we prepared a modified neutralizer broth, consisting of trypticase soy broth containing 15% tween 80, 1% soybean lecithin and 0.5% sodium thiosulfate. the susceptibilities of serratia marcescens no. 26 to 4 antiseptics obtained from the turbidity method showed a good agreement with th ... | 2002 | 12011516 |
| production of specific copolymers of polyhydroxyalkanoates from industrial waste. | polyhydroxyalkanoates, biodegradable plastics with the desired physical and chemical properties of conventional synthetic plastics, are extensively investigated. in this study, specific bacterial strains produced specific copolymers from food waste. copolymers of hb and hv (poly[3-hydroxybutyrate-co-3-hydroxyvalerate]) were obtained using various ratios of butyric acid (c4) and valeric acid (c5) as carbon sources. the c4 to c5 ratio affected the melting points of the copolymers. melting and glas ... | 2002 | 12018290 |
| detection of a variant metallo-beta-lactamase, imp-10, from two unrelated strains of pseudomonas aeruginosa and an alcaligenes xylosoxidans strain. | the gene bla(imp-10) of a variant metallo-beta-lactamase, imp-10, had a single base replacement of g by t at nucleotide 145, which led to an amino acid alteration of val49 to phe compared to the imp-1 enzyme, indicating that imp-10 was a point mutation derivative of imp-1. highly purified enzymes revealed that imp-10 was different from imp-1 in its extremely low hydrolyzing activities for penicillins, such as benzylpenicillin, ampicillin, and piperacillin. | 2002 | 12019129 |
| the iron-containing superoxide dismutase of ralstonia metallidurans ch34. | the iron-containing superoxide dismutase (fe-sod) of ralstonia metallidurans ch34 was purified and characterised as a homodimer of 2 x 21500 da containing one iron atom per monomer and exhibiting all the characteristics of the prokaryotic fe-sods except for a higher isoelectric point. the protein was 2-fold overexpressed in the presence of selenite, zinc or paraquat. r. metallidurans ch34 was suggested to contain a gene encoding for a manganese-containing sod located in the inducible chromate re ... | 2002 | 12023089 |
| autecological properties of 3-chlorobenzoate-degrading bacteria and their population dynamics when introduced into sediments. | ecologically significant properties of wild-type and genetically engineered bacteria capable of degrading 3-chlorobenzoate (3-cb) were compared in the laboratory, and isolates were introduced into streambed sediments in microcosms to observe their population dynamics. 3-cb metabolism, growth on algal extract, temperature optima, and ingestion by protozoa were ecological properties considered relevant to the persistence of these bacteria if introduced into nature. cell-specific vmax for 3-cb meta ... | 2002 | 12023727 |
| diversity of pseudomonas spp. isolated from rice rhizosphere populations grown along a salinity gradient. | along the coastline of tamil nadu, five sites were chosen to assess the diversity of pseudomonas populations isolated from rice (oryza sativa) cultivated along a salinity gradient. one of these sites was under organic farming while the other four were under inorganic farming. a total of 256 pseudomonas strains isolated from these five sites were analyzed using both phenotypic (substrate utilization patterns and antibiotic resistance assay) and genotypic (pcr-rflp of 16s rdna) characteristics. th ... | 2002 | 12023735 |
| [the bioutilization of thiodiglycol (a breakdown product of mustard gas): isolation of degraders and investigation of degradation conditions]. | the alcaligenes xylosoxydans subsp. denitrificans strain td1 capable of degrading thiodiglycol (tdg), a breakdown product of mustard gas, was isolated from soil contaminated with breakdown products of this chemical warfare agent. the selected stable variant of td1 (strain td2) can grow on tdg with a lag phase of 4-8 h and a specific growth rate of 0.04-0.045 h-1. optimal conditions for the biodegradation of tdg (ph, the concentration of tdg in the medium, and its relative content with respect to ... | 2002 | 12024827 |
| opportunistic colonization of ralstonia solanacearum-infected plants by acinetobacter sp. and its natural competence development. | the behavior of the soil bacterium acinetobacter sp. bd413 was monitored in ralstonia solanacearum-infected and non-infected tomato plants after direct injection into the stem or natural infection by roots. in healthy plants, acinetobacter sp. bd413 failed to colonize plant tissue. in plants infected simultaneously by the pathogen r. solanacearum,the acinetobacter population increased linearly to about 3.1 x 10(7) cells per gram plant material and was maintained at a high level until the death o ... | 2002 | 12037607 |
| fluoranthene degradation in pseudomonas alcaligenes pa-10. | pseudomonas alcaligenes strain pa-10 degrades the four-ring polycyclic aromatic hydrocarbon fluoranthene, co-metabolically. hplc analysis of the growth medium identified four intermediates, 9-fluorenone-1-carboxylic acid; 9-hydroxy-1-fluorene carboxylic acid; 9-fluorenone and 9-fluorenol, formed during fluoranthene degradation. pre-exposure of pa-10 to 9-fluorenone-1-carboxylic acid and 9-hydroxy-1-fluorene-carboxylic acid resulted in increases in fluoranthene removal, while pre-exposure to 9-fl ... | 2001 | 12051645 |
| bacteria-degraders as the base of an amperometric biosensor for detection of anionic surfactants. | several strains belonging to genera pseudomonas and achromobacter and characterized by the ability to degrade anionic surfactants were tested as potential bases of microbial biosensors for surfactant detection. for each strain the substrate specificity and stability of sensor signals were studied. the total amount of the substrates tested (including carbohydrates, alcohols, aromatics, organic acids, etc.) was equal to 60; the maximal signals were observed towards the anionic surfactants. the low ... | 2002 | 12052348 |
| a broad host range plasmid vector that does not encode replication proteins. | the 640-bp minimal replication region derived from a plasmid dna preparation from an acidothiobacillus ferrooxidans strain capable of autonomous replication in a range of gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, acinetobacter calcoaceticus and alcaligenes faecalis) was identified. this dna fragment (named tfk replicon) does not encode rep proteins and appears to be unrelated to other known replicons. | 2002 | 12052556 |
| dithionite reduction kinetics of the dissimilatory copper-containing nitrite reductase of alcalegenes xylosoxidans. the so(2)(.-) radical binds to the substrate binding type 2 copper site before the type 2 copper is reduced. | we report here the first detailed study of the dithionite reduction kinetics of a copper-containing dissimilatory nitrite reductase (nir). the reduction of the blue type 1 copper (t1cu) center of nir preparations that contained both type 1 and type 2 copper atoms, followed biphasic kinetics. in contrast, nir that was deficient in type 2 copper (t2dnir), followed monophasic kinetics with a second-order rate constant (t2d)k = 3.06 x 10(6) m(-1) s(-1). in all cases the so(2)(.-) radical rather than ... | 2002 | 12082116 |
| characterization of polychlorinated biphenyl-degrading bacteria isolated from contaminated sites in czechia. | biphenyl-utilizing polychlorinated biphenyls (pcb)-degrading bacteria were isolated from sites highly contaminated by pcbs, and their degradation abilities were determined using gc for typical commercial pcb mixtures (delor 103 and delor 106). out of twelve strains which utilized biphenyl as a sole source of carbon and energy, strains pseudomonas alcaligenes kp2 and p. fluorescens kp12, characterized by the biolog identification system and the neferm test, were shown to significantly co-metaboli ... | 2002 | 12094733 |
| antibiotic resistance of gram-negative bacteria in rivers, united states. | bacteria with intrinsic resistance to antibiotics are found in nature. such organisms may acquire additional resistance genes from bacteria introduced into soil or water, and the resident bacteria may be the reservoir or source of widespread resistant organisms found in many environments. we isolated antibiotic-resistant bacteria in freshwater samples from 16 u.s. rivers at 22 sites and measured the prevalence of organisms resistant to beta-lactam and non-beta-lactam antibiotics. over 40% of the ... | 2002 | 12095440 |
| [species composition and microbial contamination of atmospheric humidity condensate and potable water from the mir water supply systems during mir main missions 4 through 27]. | analyzed were quantitative and qualitative characteristics, and processes of formation and transformation of microflora in the atmospheric humidity condensate (ahc) and potable water (pw) regenerated from the condensate by the long-operating system (srv-k) aboard space station mir. the paper presents data on species composition of microflora of ahc, and pw regenerated by srv-k in the period from 1989 through to 1999. the following microbial species were identified in ahc: alcaligenes (14.8%), ba ... | 2002 | 12098948 |
| alcaligenes infection in cystic fibrosis. | the aim of this study was to investigate the effect of chronic alcaligenes species infection of the respiratory tract on the clinical status of patients with cystic fibrosis. we conducted a retrospective case-controlled study. the microbiological records of all patients attending the leeds regional pediatric and adult cystic fibrosis units from 1992-1999 were examined. chronic alcaligenes infection was defined as a positive sputum culture on at least three occasions over a 6-month period. these ... | 2002 | 12112775 |
| active site titration as a tool for the evaluation of immobilization procedures of penicillin acylase. | native and immobilized preparations of penicillin acylase from escherichia coli and alcaligenes faecalis were studied using an active site titration technique. knowledge of the number of active sites allowed the calculation of the average turnover rate of the enzyme in the various preparations and allowed us to quantify the contribution of irreversible inactivation of the enzyme to the loss of catalytic activity during the immobilization procedure. in most cases a loss of active sites as well as ... | 2002 | 12115439 |
| bioavailability of zinc in runoff water from roofing materials. | corrosion and runoff from zinc-coated materials and outdoor structures is an important source for the dispersion of zinc in the environment. being part of a large inter-disciplinary research project, this study presents the bioavailability of zinc in runoff water immediately after release from the surface of 15 different commercially available zinc-based materials exposed to the urban environment of stockholm, sweden. runoff water was analysed chemically and evaluated for its possible environmen ... | 2002 | 12137040 |
| [intestinal microflora in rats under the conditions of a chronic toxic lesion of the liver]. | intestinal microflora in healthy rats and its changes under the conditions of experimental chronic toxic hepatitis were studied. the study revealed that in intact animals the microflora of the small intestine was represented by bacteria of the genera escherichia, enterobacter, moraxella, alcaligenes, staphylococcus, streptococcus. bacteria of the genera escherichia, enterobacter, moraxella, alcaligenes, staphylococcus, corynebacterium and clostridium were isolated from the large intestine. no ba ... | 2002 | 12141042 |
| use of a co2 electrode to monitor fermentations that use xenobiotic compounds as source of carbon. | bacterial strains were isolated from contaminated waters, mud or soils. they are capable of growing in mineral medium with different chemicals as carbon source, such as aliphatic or aromatic hydrocarbons and polychlorinated biphenyls (pcbs). most of these strains tolerate high concentrations (up to 30% v/v) of the xenobiotic substrates. this is particularly important for the development of fermenting processes to treat effluents or residues with a high content of contaminating compounds. an ion- ... | 2002 | 12180260 |
| characterization of copolymer hydroxybutyrate/hydroxyvalerate from saponified vernonia, soybean, and "spent" frying oils. | poly(beta-hydroxyalkanoate)s (phas) were biosynthesized by ralstonia eutropha (formerly known as alcaligenes eutrophus) by using saponified soybean, vernonia, and "spent" frying oils. these phas were isolated and characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms), gas chromatography/mass spectrometry (gc/ms), proton nuclear magnetic resonance spectrometry (1h nmr), and 2-dimensional homonuclear (1h-1h) correlation spectroscopy (cosy). the ... | 2002 | 12180688 |
| electrostatic role of aromatic ring stacking in the ph-sensitive modulation of a chymotrypsin-type serine protease, achromobacter protease i. | achromobacter protease i (api) has a unique region of aromatic ring stacking with trp169-his210 in close proximity to the catalytic triad. this paper reveals the electrostatic role of aromatic stacking in the shift in optimum ph to the alkaline region, which is the highest ph range (8.5-10) among chymotrypsin-type serine proteases. the ph-activity profile of api showed a sigmoidal distribution that appears at ph 8-10, with a shoulder at ph 6-8. variants with smaller amino acid residues substitut ... | 2002 | 12180992 |
| identification of bacteria in drinking and purified water during the monitoring of a typical water purification system. | a typical purification system that provides purified water which meets ionic and organic chemical standards, must be protected from microbial proliferation to minimize cross-contamination for use in cleaning and preparations in pharmaceutical industries and in health environments. | 2002 | 12182763 |
| genetic analysis of pigment biosynthesis in xanthobacter autotrophicus py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria. | a highly efficient method of transposon mutagenesis was developed for genetic analysis of xanthobacter autotrophicus py2. the method makes use of a transposon delivery vector that encodes a hyperactive tn 5 transposase that is 1,000-fold more active than the wild-type transposase. in this construct, the transposase is expressed from the promoter of the teta gene of plasmid rp4, which is functional in a wide variety of organisms. the transposon itself contains a kanamycin resistance gene as a sel ... | 2002 | 12189420 |
| crystallization and preliminary crystallographic analysis of a d-aminoacylase from alcaligenes faecalis da1. | d-aminoacylases catalyze the hydrolysis of n-acyl-d-amino acids into d-amino acids with the aid of zinc ions. the first d-aminoacylase crystal from alcaligenes faecalis has been obtained in hanging drops at ph 5.6 by the vapour-diffusion method using 30% polyethylene glycol 4000 as precipitant. it belongs to space group p2(1)2(1)2(1), with unit-cell parameters a = 60.2, b = 76.6, c = 135.3 a. reflections to 1.2 a resolution are observable. an initial atomic model with 472 residues has been built ... | 2002 | 12198309 |
| revisiting the kinetics of nitric oxide (no) binding to soluble guanylate cyclase: the simple no-binding model is incorrect. | soluble guanylate cyclase (sgc) is a ferrous iron hemoprotein receptor for nitric oxide (no). no binding to the heme activates the enzyme 300-fold. sgc as isolated is five-coordinate, ferrous with histidine as the axial ligand. the no-activated enzyme is a five-coordinate nitrosyl complex where the axial histidine bond is broken. past studies using rapid-reaction kinetics demonstrated that both the formation of a six-coordinate intermediate and the conversion of the intermediate to the activated ... | 2002 | 12209005 |
| imaging the proteins pseudoazurin and apo-pseudoazurin on gold by stm in air: effect of the bias voltage. | we have applied scanning tunnelling microscopy (stm) to the study of two proteins: pseudoazurin and apo-pseudoazurin. both proteins adsorbed onto a au (1 1 1) surface are visible to stm individually, forming into layers and multilayers, with currents from about 55 to 600 pa. the images reproduce well the expected dimensions laterally but not in the z direction. the apparent height of the proteins varies with the voltage polarity, being higher at negative sample voltages. the bias also affects th ... | 2002 | 12211474 |
| use of potassium tellurite for testing the survival and viability of pseudomonas pseudoalcaligenes kf707 in soil microcosms contaminated with polychlorinated biphenyls. | this study shows that the oxyanion tellurite teo3(2-) can be used as a tool to detect and quantify the release in soil microcosms of pseudomonas pseudoalcaligenes kf707, a strain spontaneously resistant to tellurite with a minimal inhibitory concentration (mic) of 150 microg ml(-1). kf707 cells which carry the genes for degradation of a wide range of polychlorinated biphenyl congeners (pcbs) were used for inoculation of laboratory microcosms prepared with two different pcb-contaminated soils (ci ... | 2002 | 12234009 |
| pathogenic effects of bacteria isolated from larvae of hylesia metabus crammer (lepidoptera: saturniidae). | hylesia metabus larvae are susceptible to several pathogens indigenous to the area in which they are found. some larvae show symptoms characteristic of bacterial infection; they become flaccid and lethargic, and show a marked loss of appetite. we isolated and identified 29 bacterial strains from live, dead and experimentally infected h. metabus larvae, and evaluated their pathogenic activity. the bacteria which caused mortality in the larvae were: pseudomonas aeruginosa (60-93.3%), proteus vulga ... | 2002 | 12234536 |
| the active site of arsenite oxidase from alcaligenes faecalis. | arsenite oxidase, a member of the dmso reductase family of molybdenum enzymes, has two molecules of guanosine dinucleotide molybdenum cofactor coordinating the molybdenum at the active site. x-ray absorption spectroscopy indicates that the mo-s bonds shorten from 2.47 to 2.37 a upon reduction with the physiological substrate. it also indicates the presence of an oxo ligand at 1.70 a in both oxidized and reduced forms of the enzyme, together with a short, 1.83 a, mo-o bond in the oxidized form th ... | 2002 | 12236735 |
| [use of maleic acid by mixed cultures of microorganisms]. | in a mixed batch culture, alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed maleic acid into malic acid. bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. both bacterial cultures dissociated with the formation of r, s, and m forms. at a concentration of 5.0 g/l, maleic acid was utilized maximally by rs and ss forms of the association a. xylosoxidans and bacillus subtilis. at concentrations 15.0 and 25.0 g/l, maleic acid was u ... | 2002 | 12325296 |
| accumulation of poly(3-hydroxybutyrate) from octanoate in different pseudomonas belonging to the rrna homology group i. | it is admitted that one of the characteristics of pseudomonads is their inability to accumulate poly(3-hydroxybutyrate). in this paper, we show that poly(3-hydroxyoctanoate) synthesis is restricted to pseudomonas rrna homology group i, which includes both fluorescent and nonfluorescent species. however, within the genus pseudomonas, the p. aeruginosa complex can be subdivided into two groups: the "p. aeruginosa group", which includes p. aeruginosa, p. alcaligenes, p. citronellolis, p. mendocina, ... | 2002 | 12353870 |
| microbial diversity in an in situ reactor system treating monochlorobenzene contaminated groundwater as revealed by 16s ribosomal dna analysis. | a molecular approach based on the construction of 16s ribosomal dna clone libraries was used to investigate the microbial diversity of an underground in situ reactor system filled with the original aquifer sediments. after chemical steady state was reached in the monochlorobenzene concentration between the original inflowing groundwater and the reactor outflow, samples from different reactor locations and from inflowing and outflowing groundwater were taken for dna extraction. small-subunit rrna ... | 2002 | 12353878 |
| identification and characterization of a new gene from variovorax paradoxus iso1 encoding n-acyl-d-amino acid amidohydrolase responsible for d-amino acid production. | an n-acyl-d-amino acid amidohydrolase (n-d-aaase) was identified in cell extracts of a strain, iso1, isolated from an environment containing n-acetyl-d-methionine. the bacterium was classified as variovorax paradoxus by phylogenetic analysis. the gene was cloned and sequenced. the gene consisted of a 1467-bp orf encoding a polypeptide of 488 amino acids. the v. paradoxusn-d-aaase showed significant amino acid similarity to the n-acyl-d-amino acid amidohydrolases of the two eubacteria alcaligenes ... | 2002 | 12354118 |
| use of 16s rrna gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center. | during 1999, we used partial 16s rrna gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. these 46 isolates were genotypically identified as pseudomonas aeruginosa (19 isolates, 12 patients), achromobacter xylosoxidans (10 isolates, 8 ... | 2002 | 12354883 |
| structural analysis of the curdlan-like exopolysaccharide produced by cellulomonas flavigena ku. | cellulomonas flavigena ku produces large quantities of an insoluble exopolysaccharide (eps) under certain growth conditions. the eps has previously been shown to be a glucose polymer and to have solubility properties similar to curdlan, a beta-1,3-d-glucan produced by alcaligenes faecalis var. myxogenes 10c3k. furthermore, eps purified by alkaline extraction stains with aniline blue, a dye specific for curdlan-type polysaccharides. however, eps-producing colonies of c. flavigena ku do not stain ... | 2002 | 12355320 |
| sulphoacetaldehyde acetyltransferase yields acetyl phosphate: purification from alcaligenes defragrans and gene clusters in taurine degradation. | the facultatively anaerobic bacterium alcaligenes defragrans nkntau was found to oxidize taurine (2-aminoethanesulphonate) with nitrate as the terminal electron acceptor. taurine was transaminated to 2-sulphoacetaldehyde. this was not converted into sulphite and acetate by a "sulphoacetaldehyde sulpho-lyase" (ec 4.4.1.12), but into sulphite and acetyl phosphate, which was identified by three methods. the enzyme, which required the addition of phosphate, thiamin diphosphate and mg(2+) ions for ac ... | 2003 | 12358600 |
| alcaligenes xylosoxidans subspecies denitrificans pseudobacteraemia in a university children's hospital. | | 2002 | 12372332 |
| structural-based mutational analysis of d-aminoacylase from alcaligenes faecalis da1. | d-aminoacylase is an attractive candidate for commercial production of d-amino acids through its catalysis in the zinc-assistant hydrolysis of n-acyl-d-amino acids. we report here the cloning, expression, and structural-based mutation of the d-aminoacylase from alcaligenes faecalis da1. a 1,007-bp pcr product amplified with degenerate primers, was used to isolate a 4-kb genomic fragment, encoding a 484-residue d-aminoacylase. the enzyme amino-terminal segment shared significant homology within a ... | 2002 | 12381838 |
| microbiological evaluation of a commercial transport system for urine samples. | the aim of this study was to evaluate a commercial tube prepared with boric acid, sodium formate and sorbitol (hemogard vacutainer tubes, becton-dickinson, hg tubes). fourteen bacterial strains were incubated in urine in hg tubes and conventional tubes. during a 24-h period, most of the microorganisms grew readily in conventional tubes at room temperature, whereas the bacterial counts were comparatively unchanged when chilled or kept in hg tubes. the bacterial counts of alcaligenes faecalis and ... | 2002 | 12387577 |
| purple urine bag syndrome. | | 2002 | 12429328 |
| metal-ligand interplay in blue copper proteins studied by 1h nmr spectroscopy: cu(ii)-pseudoazurin and cu(ii)-rusticyanin. | the blue copper proteins (bcps), pseudoazurin from achromobacter cycloclastes and rusticyanin from thiobacillus ferrooxidans, have been investigated by (1)h nmr at a magnetic field of 18.8 t. hyperfine shifts of the protons belonging to the coordinated ligands have been identified by exchange spectroscopy, including the indirect detection for those resonances that cannot be directly observed (the beta-ch(2) of the cys ligand, and the nh amide hydrogen bonded to the s(gamma)(cys) atom). these dat ... | 2002 | 12431099 |
| metabolism of ddt [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] by alcaligenes denitrificans itrc-4 under aerobic and anaerobic conditions. | an isolated bacterium, alcaligenes denitrificans itrc-4, metabolizes 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt) under both aerobic and anaerobic conditions. the aerobic metabolism is inhibited by 38% and 47% in the presence of 1.0 g l(-1) of sodium acetate and sodium succinate, respectively, but remains uninhibited in the presence of 1.0 g l(-1) of glucose. also, the metabolism is inhibited completely in the presence of biphenyl vapors, as well as 0.8 g l(-1) of 2,2'-bipyridyl. under an ... | 2003 | 12432467 |
| preliminary crystallographic studies of two c-terminally truncated copper-containing nitrite reductases from achromobacter cycloclastes: changed crystallizing behaviors caused by residue deletion. | the c-terminal segment of copper-containing nitrite reductase from achromobacter cycloclastes (acnir) has been found essential for maintaining both the quaternary structure and the enzyme activity of acnir. c-terminal despentapeptide acnir (nirc-5) and desundecapeptide acnir (nirc-11) are two important truncated mutants whose activities and stability have been affected by residue deletion. in this study, the two mutants were crystallized using the hanging drop vapor diffusion method. crystals of ... | 2002 | 12437965 |
| runoff rates, chemical speciation and bioavailability of copper released from naturally patinated copper. | the release of copper, induced by atmospheric corrosion, from naturally patinated copper of varying age (0 and 30 years) has been investigated together with its potential ecotoxic effect. results were generated in an interdisciplinary research effort in which corrosion science and ecotoxicology aspects were combined. the aim of the investigation was to elucidate the situation when copper-containing rainwater leaves a roof in terms of runoff rate, chemical speciation, bioavailability and ecotoxic ... | 2002 | 12442792 |
| degradation of aliphatic polyester films by commercially available lipases with special reference to rapid and complete degradation of poly(l-lactide) film by lipase pl derived from alcaligenes sp. | commercial lipases were examined for their degradation efficiency of aliphatic polyester films. in 100 days immersion of polyester films in lipase solutions at 37 degrees c at ph 7.0, lipase asahi derived from chromobacterium viscosum degraded polybutylene succinate-co-adipate (pbsa), poly (e-caprolactone) (pcl) and polybutylene succinate (pbs), and lipase f derived from rhizopus niveus degraded pbsa and pcl during 4-17 days. lipase f-ap15 derived from rhizopus orizae could degrade pbsa in 22 da ... | 2002 | 12449316 |
| [thiodiglycol metabolism in alcaligenes xylosoxydans subsp. denitrificans]. | the investigation of the degradation of thiodiglycol (the major product of mustard gas hydrolysis) by alcaligenes xylosoxydans subsp. denitrificans strain td2 showed that thiodiglycol is metabolized through the oxidation of its primary alcohol groups and the subsequent cleavage of c-s bonds in the intermediate products, thiodiglycolic and thioglycolic acids. the end products of these reactions are so4(2-) ions and acetate, the latter being involved in the central metabolism of strain td2. the ox ... | 2002 | 12449625 |
| [persistent properties of protozoa-associated bacteria]. | the species structure and persistent properties (antilysozyme and antihistone activity) of bacteria forming associations with protozoa is revealed. among them, 68.9% of the isolates were enterobacteria, the remaining organisms belonged to the families aeromonas, alcaligenes, pseudomonas, vibrio, etc. within the family enterobacteriaceae bacteria of the escherichia group prevailed. 50.4% of the isolates were found to have antilysozyme activity and 97%--antihistone activity. the level of persisten ... | 2002 | 12449703 |
| a novel high-cell-density protein expression system based on ralstonia eutropha. | we describe the development of a novel protein expression system based on the industrial fermentation organism ralstonia eutropha (formerly known as alcaligenes eutrophus) ncimb 40124. this new system overcomes some of the shortcomings of traditional escherichia coli-based protein expression systems, particularly the propensity of such systems to form inclusion bodies during high-level expression. using a proteomics approach, we identified promoters that can be induced by simple process paramete ... | 2002 | 12450812 |
| crystal structure of d-aminoacylase from alcaligenes faecalis da1. a novel subset of amidohydrolases and insights into the enzyme mechanism. | d-aminoacylase is an attractive candidate for commercial production of d-amino acids through its catalysis in the hydrolysis of n-acyl-d-amino acids. we report here the first d-aminoacylase crystal structure from a. faecalis at 1.5-a resolution. the protein comprises a small beta-barrel, and a catalytic (betaalpha)(8)-barrel with a 63-residue insertion. the enzyme structure shares significant similarity to the alpha/beta-barrel amidohydrolase superfamily, in which the beta-strands in both barrel ... | 2003 | 12454005 |
| molecular characterization of a salt-tolerant bacterial community in the rice rhizosphere. | the diversity of salt-tolerant bacteria present in the rhizosphere of oryza sativa was investigated. fourteen bacterial strains, isolated after enrichment in nitrogen-free, semi-solid medium and showing tolerance to 3% nacl, were analyzed by restriction patterns produced by amplified dna coding for 16s rdna (ardra) with enzymes sau3ai, alui and rsai which showed that they were represented by 4 ardra types. biodiversity among the 14 strains was also analyzed by the random amplified polymorphic dn ... | 2002 | 12455705 |
| antimicrobial activity of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), compared to other quinolones, against clinical isolates from cancer patients. | the in vitro spectrum of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), was compared with that of ciprofloxacin, levofloxacin, and trovafloxacin against 736 clinical isolates from cancer patients. garenoxacin was the most active agent overall against gram-positive organisms, with potent activity against aerococcus spp., micrococcus spp., rhodococcus equi, stomatococcus mucilaginous, bacillus spp., enterococcus faecalis, listeria monocytogenes, methicillin-susceptible staphylococcus ... | 2002 | 12458127 |
| metal-ligand interactions in perturbed blue copper sites: a paramagnetic (1)h nmr study of co(ii)-pseudoazurin. | pseudoazurin is an electron transfer copper protein, a member of the cupredoxin family. the protein is frequently found in denitrifying bacteria, where it is the electron donor of nitrite reductase. the copper at the active site is coordinated to his40, cys78, his81 and met86 in a distorted tetragonal geometry. we have recorded and assigned the (1)h nmr spectra of co(ii)-substituted pseudoazurin from achromobacter cycloclastes. the (1)h nmr spectrum of co(ii)-pseudoazurin closely resembles that ... | 2003 | 12459901 |
| development and characterization of murine monoclonal antibodies specific for dissimilatoric copper nitrite reductase. | several hybridoma cell lines from mice were established, producing monoclonal antibodies (mabs) directed against the dissimilatoric copper nitrite reductase (dnir) to detect actual denitrifying bacteria at the single cell level under nondestructive conditions in the environment. the mice were immunized with native or recombinant enzyme gained from two different bacteria, ochrobactrum anthropi and alcaligenes faecalis. the antibodies obtained could be divided into two groups according to their di ... | 2002 | 12470477 |
| arsenite oxidase aox genes from a metal-resistant beta-proteobacterium. | the beta-proteobacterial strain ulpas1, isolated from an arsenic-contaminated environment, is able to efficiently oxidize arsenite [as(iii)] to arsenate [as(v)]. mutagenesis with a lacz-based reporter transposon yielded two knockout derivatives deficient in arsenite oxidation. sequence analysis of the dna flanking the transposon insertions in the two mutants identified two adjacent open reading frames, named aoxa and aoxb, as well as a putative promoter upstream of the aoxa gene. reverse transcr ... | 2003 | 12486049 |
| a reactor system combining reductive dechlorination with co-metabolic oxidation for complete degradation of tetrachloroentylene. | a laboratory sequential anaerobic-aerobic bioreactor system, which consisted of an anaerobic fixed film reactor and two aerobic chemostats, was set up to degrade tetrachloroethylene (pce) without accumulating highly toxic degradation intermediates. a soil enrichment culture, which could reductively dechlorinate 900 microm (ca. 150 mg/l) of pce stoichiometrically into cis-1,2-dichloroethylene (cis-dce), was attached to ceramic media in the anaerobic fixed film reactor. a phenol degrading strain, ... | 2002 | 12491716 |
| precipitin response of potato processing workers to work-related microbial allergens. | serum samples from 61 potato processing workers and 30 urban dwellers not exposed to organic dusts (as a reference group) were examined in agar-gel precipitation test performed by ouchterlony double diffusion method with the antigens of 12 microorganisms associated with organic dusts. each serum was tested twice: not concentrated, and three-fold concentrated, for the detection of low levels of precipitins. the antibody response of workers to the antigen of coryneform bacterium agromyces ramosus ... | 2002 | 12498593 |
| purification and characterization of beta-1,3-xylanase from a marine bacterium, alcaligenes sp. xy-234. | a beta-1,3-xylanase-producing bacterium, alcaligenes sp. xy-234, was isolated from the marine environment. the organism produced endo-1,3-beta-xylanase at a high level in the culture fluid. the enzyme was purified 292-fold by ammonium sulfate precipitation and several column chromatographies. the final enzyme preparation appeared to be homogeneous on disc gel electrophoresis and sds-page with a molecular mass of 59 kda, and the pi was 4.0. the enzyme hydrolyzed beta-1,3-xylan and larger xyloolig ... | 1998 | 12501421 |
| evaluation of microscan autoscan for identification of pseudomonas aeruginosa isolates from cystic fibrosis patients. | accurate identification of gram-negative bacilli from cystic fibrosis (cf) patients is essential. only 57% (108 of 189) of nonmucoid strains and 40% (24 of 60) of mucoid strains were definitively identified as pseudomonas aeruginosa with microscan autoscan. most common misidentifications were pseudomonas fluorescens-pseudomonas putida (i.e., the strain was either p. fluorescens or p. putida, but the system did not make the distinction and yielded the result p. fluorescens/putida) and alcaligenes ... | 2003 | 12517904 |
| characterization of microbial community in nitrogen removal process of metallurgic wastewater by pcr-dgge. | the metallurgic wastewater generated from the processes of recovering precious metals from industrial wastes contains high concentrations of nitrogen compounds such as ammonia and nitric acid and of salts such as sodium chloride and sodium sulfate. biological nitrogen removal from this wastewater was attempted by a circulating bioreactor system equipped with an anoxic packed bed and an aerobic fluidized bed. the anoxic packed bed of this system was found to effectively remove nitrite and nitrate ... | 2002 | 12523738 |
| genetic analysis and characterization of poly(aspartic acid) hydrolase-1 from sphingomonas sp. kt-1. | sphingomonas sp. kt-1 hydrolyzes poly(aspartic acid) (paa) containing alpha- and beta-amide units and has at least two different types of paa hydrolases. the paa hydrolase-1 hydrolyzes selectively beta-beta amide units in paa. molecular cloning of paa hydrolase-1 from sphingomonas sp. kt-1 has been carried out to characterize its gene products. genetic analysis shows that the deduced amino acid sequence of paa hydrolase-1 has a similarity with those of the catalytic domain of poly(3-hydroxybutyr ... | 2003 | 12523851 |
| directing the mode of nitrite binding to a copper-containing nitrite reductase from alcaligenes faecalis s-6: characterization of an active site isoleucine. | unlike the heme cd(1)-based nitrite reductase enzymes, the molecular mechanism of copper-containing nitrite reductases remains controversial. a key source of controversy is the productive binding mode of nitrite in the active site. to identify and characterize the molecular determinants associated with nitrite binding, we applied a combinatorial mutagenesis approach to generate a small library of six variants at position 257 in nitrite reductase from alcaligenes faecalis s-6. the activities of t ... | 2003 | 12538888 |
| molecular diversity and characterization of nitrite reductase gene fragments (nirk and nirs) from nitrate- and uranium-contaminated groundwater. | nitrate-contaminated groundwater samples were analysed for nirk and nirs gene diversity. the samples differed with respect to nitrate, uranium, heavy metals, organic carbon content, ph and dissolved oxygen levels. a total of 958 nirk and 1162 nirs clones were screened by restriction fragment length polymorphism (rflp) analysis: 48 and 143 distinct nirk and nirs clones, respectively, were obtained. a single dominant nirk restriction pattern was observed for all six samples and was 83% identical t ... | 2003 | 12542709 |
| preliminary study of an enzyme extracted from alcaligenes sp. strain yf11 capable of degrading pesticides. | | 2003 | 12545372 |
| [tn5 mutagenesis and the characteristics of indole-3-acetic acid biosynthesis in alcaligenes faecalis a1501]. | the iaa production of alcaligenes faecalis wild type strain a1501 depended on addition of l-tryptopham(trp). a1501 could grow well in minimal medium without trp, but could not produce iaa. the results indicated that there was trp-dependent iaa biosynthesis pathway in a. faecalis a1501. a1501 shows a characteristic of cell density-dependent iaa biosynthesis. the tn5-induced mutant bank of a1501 was constructed using tn5 mutagenesis and one trp auxotrophic mutant at63 was selected and identified. ... | 2000 | 12548770 |
| [the construction of a recombined e. coli strain with stable and high production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)]. | plasmid pjmc2 was constructed by cloning the parde fragment of rk2 into ptz18u-phb which harbored phacab from alcaligenes eutrophus and was transferred into e. coli hms174 and e. coli jm107 separately. it is very stable in its hosts cultured in medium without ampicillin. e. coli hms 174(ptz18u-phb) and e. coli jm107(ptz18u-phb) produced p(3hb-co-3hb) in a low phosphate concentration medium(18 mmol/l). the proportion of 3-hydroxyvalerate(3hv) in the polymer was 5%-8%. a fed-batch culture of e. co ... | 2000 | 12548874 |
| [effects of nitrogen feeding on the accumulation of poly-beta-hydroxybutyrate with alcaligenes eutrophus]. | on the basis of analysis of phb fermentation processes, the effects of ammonium sulfate feeding rate at phb formation period on the phb accumulation by alcaligenes eutrophus were investigated. it was shown that the complete absence of nitrogen source at phb formation phase would lead to the decline of phb synthetic activity, and the obvious influences of different nitrogen feeding rate on phb synthesis were observed. higher phb content, but relative lower cell dry weight, phb concentration and p ... | 2000 | 12548994 |
| [cloning of a new catechol 1,2-dioxygenase gene (tfd c) from plesiomonas and its expression in the e. coli]. | a new catechol-1,2-dioxygenase gene (tfd c) was cloned from the plesiomonas using the pcr method. primers were designed according to the reported sequence of catechol-1,2-dioxygenase (c120) gene from alcaligenes eutroplus. the amplified fragment contained a 765 bp open reading frame (orf), encoding a protein of 255 amino acids. the new tfd c gene shared a high homology with the one cloned from alcaligenes eutroplus, showing only one base difference at 693 site (c-->a) and consequently one amino ... | 2000 | 12549050 |
| [the construction of alcaligenes faecalis ntrc-lacz fusion gene and its expression during association with rice roots]. | a broad host range vector pla2917 containing ntrc gene or ntrc-lacz fusion were constructed, namely plac1 and plac2. the plasmids plac1 and plac2 were introduced into a. faecalis wild type strain a1501 by conjugation, subsequently to abtain a15c1 and a15c2. the expression and regulation of ntrc gene of a. faecalis associated with rice roots was investigated under the condition of the associative nitrogen fixation using x-gal decoration method, micrograph and ntrc partially deletion mutant. the b ... | 1998 | 12549327 |
| [studies on microbial factor on color change of dunhuang mural. i. classification of microbes on color-changed mural and property of some typical species]. | microbial strains were isolated from 51 typical color changed samples of mural in 6 grottos of dunhuang mogao gretto. after identification, it is proved that belong to 6 genera of bacteria and 5 genera of mould. bacillus, alcaligenes and penicillium were dominant species in all of them. in the imitative experiment, it is found that cladosporium sp., a. niger and two strains of bacteria have a great effect on the color change of red pigment of mural and aging of sizing agent. | 1998 | 12549389 |
| molecular characterisation of bacteria in a wetland used to remove ammoniacal-n from landfill leachate. | bacteria associated with leachate from a constructed, surface-flow wetland effective at removing ammonium-n, were characterised using molecular methods which bypass the need for cultivation. dna was extracted from the sample and bacterial 16s rdna sequences amplified and cloned. ninety-six individual clones were re-amplified and analysed by restriction digestion and targeted sequencing. a collector's curve was used to estimate the total number of bacterial groups (operational taxonomic units, ot ... | 2002 | 12549664 |
| [studies on the parasites of paris polyphylla var. yunnanensis]. | two bacteria and three fungi were isolated from the hypogeal stems of paris polyphylla var. yunnanensis. the bacteria were identified as bacillus cereus and pseudomonas alcaligenes, and the fungi were identified as periconia sp., pachnocybe albida and hormomyces paridiphilus. the results on liquid culture of b. cereus, p. alcaligenes and h. paridiphilus indicated that the colloidization and polysaccharide content increasing in hypogeal stems of p. polyphylla var. yunnanensis were due to the extr ... | 1999 | 12555422 |
| [optimization of fermentation conditions for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with alcaligenes eutrophus]. | a close relationship, between the initial addition time and concentration of propionate and hv fraction, was observed in shaking culture of alcaligenes eutrophus for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate). the optimal initial addition time of propionate was determined at the onset of phbv formation period. although relatively high hv unit could be obtained under high propionate concentration, the growth and product synthetic activities were inhibited obviously. different ... | 1999 | 12555542 |
| [effects of h2o2 addition on oxygen supply and metabolism of microorganisms]. | | 2002 | 12557363 |
| [cloming, sequence analysis of imidase gene from alcaligenes eatrophus and its expression in e. coli]. | a hydantoin-cleaving microorganism 112r4 is screened and identified to be alcaligenes eutrophus. the resting cell of alcaligenes eutrophus 112r4 can catalyze the hydrolysis of hydantoin, dihydropyrimidine and succinimide effectively, but not function to 5-monosubstituted hydantoins or 5,5'-disubstituted hydantoins. the microorganism can utilize succinimide as a sole carbon source and nitrogen source, which indicates the presence of a complete transformation pathway of succinimide, and a hydantoi ... | 2002 | 12557390 |
| [influence of ph control on the production of curdlan by alcaligenes faecalis strain]. | a two-stage ph control method was employed in batch fermentation of curdlan by alcaligenes faecalis wx-c12 where cell-growth stage was constantly controlled at ph 7.0 and stationary stage was controlled at a constant ph as well. the influence of ph control on the curdlan production was investigated. the optimal ph control of batch process for curdlan production was obtained when cell-growth stage was controlled at ph 7.0 and stationary stage was constantly controlled at ph 5.6. production and pr ... | 2002 | 12561215 |