| crystal structure of the novel paib transcriptional regulator from geobacillus stearothermophilus. | | 2011 | 21633969 |
| cloning, expression, purification, crystallization and preliminary x-ray crystallographic study of gk0767, the copper-containing nitrite reductase from geobacillus kaustophilus. | the soluble region (residues 32-354) of gk0767, a copper-containing nitrite reductase from the thermophilic gram-positive bacterium geobacillus kaustophilus hta426, has been cloned and overexpressed in escherichia coli. the purified recombinant protein was crystallized using the hanging-drop vapour-diffusion method. x-ray diffraction data were collected and processed to a maximum resolution of 1.3 å. the crystals belonged to space group r3, with unit-cell parameters a = b = 115.1, c = 87.5 å. pr ... | 2011 | 21636914 |
| geobacillus galactosidasius sp. nov., a new thermophilic galactosidase-producing bacterium isolated from compost. | two thermophilic spore-forming strains, with optimum growth temperature at 70°c, were isolated from compost of the "experimental system of composting" (teora, avellino, italy). a phylogenetic analysis based on 16s rrna gene sequences showed that these organisms represented a new species of the genus geobacillus. based on polyphasic taxonomic data the strains represented a novel species for which the name geobacillus galactosidasius sp. nov. is proposed. the type strain is cf1b(t) (=atcc baa-1450 ... | 2011 | 21645983 |
| amyloid histology stain for rapid bacterial endospore imaging. | bacterial endospores are some of the most resilient forms of life known to us, with their persistent survival capability resulting from a complex and effective structural organization. the outer membrane of endospores is surrounded by the densely packed endospore coat and exosporium, containing amyloid or amyloid-like proteins. in fact, it is the impenetrable composition of the endospore coat and the exosporium that makes staining methodologies for endospore detection complex and challenging. th ... | 2011 | 21653779 |
| isolation and characterization of arsenic resistant geobacillus kaustophilus strain from geothermal soils. | a thermophilic, arsenate resistant bacterial strain was isolated from a geothermal field located in the area surrounding monterotondo (tuscany, italy). based on 16s rrna gene analysis and recn comparisons the strain was identified as geobacillus kaustophilus. cells of the strain, designated a1, were rod-shaped, 2-3 μm long and reacted negatively to gram staining, despite its taxonomic classification as a gram positive microorganism. strain a1 is a thermophilic spore-forming bacterium, and grows ... | 2011 | 21656800 |
| identification of the substrate binding site in the n-terminal tbp-like domain of rnase h3. | ribonuclease h3 from bacillus stearothermophilus (bst-rnase h3) has the n-terminal tbp-like substrate-binding domain. to identify the substrate binding site in this domain, the mutant proteins of the intact protein and isolated n-domain, in which six of the seventeen residues corresponding to those involved in dna binding of tbp are individually mutated to ala, were constructed. all of them exhibited decreased enzymatic activities and/or substrate-binding affinities when compared to those of the ... | 2011 | 21664908 |
| simultaneous hydrocarbon biodegradation and biosurfactant production by oilfield-selected bacteria. | aims:ôçé to study the bacterial diversity associated with hydrocarbon biodegradation potentiality and biosurfactant production of tunisian oilfields bacteria. methods and results:ôçé eight tunisian hydrocarbonoclastic oilfields bacteria have been isolated and selected for further characterization studies. phylogenetic analysis revealed that three thermophilic strains belonged to the genera geobacillus, bacillus and brevibacillus, and that five mesophilic strains belonged to the genera pseudomona ... | 2011 | 21668593 |
| splint sterilization-a potential registration hazard in computer-assisted surgery. | purpose: registration of preoperative targeting information for the intraoperative situation is a crucial step in computer-assisted surgical interventions. point-to-point registration using acrylic splints is among the most frequently used procedures. there are, however, no generally accepted recommendations for sterilization of the splint. an appropriate method for the thermolabile splint would be hydrogen peroxide-based plasma sterilization. this study evaluated the potential deformation of th ... | 2011 | 21813224 |
| trimer-based design of ph-responsive protein cage results in soluble disassembled structures. | limited studies have been done on the interactions between subunits of self-assembling protein cages. e2 protein cage from bacillus stearothermophilus was investigated in this work to impart ph-sensitive disassembly profile. key amino acids were identified at the intratrimer and intertrimer interfaces, and histidine residues were introduced to these key sites to probe for their influences on the e2 assembly. we found that both the intratrimer- and the intertrimer-modified mutant proteins have th ... | 2011 | 21797220 |
| crystallization and preliminary crystallographic analysis of the phosphotriesterase-like lactonase from geobacillus kaustophilus. | gk1506 from the thermophilic bacterium geobacillus kaustophilus is a member of the phosphotriesterase-like lactonases, which can catalyze the hydrolysis of a broad range of compounds with different chemical properties. it is of particular interest because of its high thermostability and its dual activity towards organophosphate compounds and some lactones. these properties make gk1506 an attractive target for future enzyme engineering and use in practical applications. in order to resolve the cr ... | 2011 | 21795796 |
| design of a coupled bioluminescent assay for a recombinant pyruvate kinase from a thermophilic geobacillus. | a simple and rapid method using coupled bioluminescent assay was developed to determine level of adp. adp is involved in many biological reactions and adp assay can be used for assaying some reactions universally by monitoring adp formation or depletion. adp analysis involves incubation of adp or extracts containing adp with pyruvate kinase (pk) and pep. the atp formed by this reaction is determined by measuring the intensity of the initial light flash produced when luciferin-luciferase preparat ... | 2011 | 21790618 |
| crystal structures and repair studies reveal the identity and the base-pairing properties of the uv-induced spore photoproduct dna lesion. | uv light is one of the major causes of dna damage. in spore dna, due to an unusual packing of the genetic material, a special spore photoproduct lesion (sp lesion) is formed, which is repaired by the enzyme spore photoproduct lyase (spl), a radical s-adenosylmethionine (sam) enzyme. we report here the synthesis and dna incorporation of a dna sp lesion analogue lacking the phosphodiester backbone. the oligonucleotides were used for repair studies and they were cocrystallized with a polymerase enz ... | 2011 | 21780197 |
| hydrocarbon degradation and bioemulsifier production by thermophilic geobacillus pallidus strains. | geobacillus pallidus xs2 and xs3 were isolated from oil contaminated soil samples in yumen oilfield, china, and were able to produce bioemulsifiers on different hydrocarbons. biodegradation assays exhibited that approximately 70% of pah (250mg/l) or 85% of crude oil (500mg/l) was removed by the thermophilic bacteria after 20days. the bioemulsifiers of the two strains were isolated and obtained a productive yield of 4.24±0.08 and 3.82±0.11g/l, respectively. gpc analysis revealed that the number-a ... | 2011 | 21764302 |
| a head-to-head comparison of hydrogen peroxide vapor and aerosol room decontamination systems. | objective.ôçânew technologies have emerged in recent years for the disinfection of hospital rooms and equipment that may not be disinfected adequately using conventional methods. there are several hydrogen peroxide-based area decontamination technologies on the market, but no head-to-head studies have been performed. design.ôçâwe conducted a head-to-head in vitro comparison of a hydrogen peroxide vapor (hpv) system (bioquell) and an aerosolized hydrogen peroxide (ahp) system (sterinis). setting. ... | 2011 | 21828962 |
| bioprocessing of agricultural residues to ethanol utilizing a cellulolytic extremophile. | a recently discovered thermophilic isolate, geobacillus sp. r7, was shown to produce a thermostable cellulase with a high hydrolytic potential when grown on extrusion-pretreated agricultural residues such corn stover and prairie cord grass. at 70-¦c and 15-20% solids, the thermostable cellulase was able to partially liquefy solid biomass only after 36-áh of hydrolysis time. the hydrolytic capabilities of geobacillus sp. r7 cellulase were comparable to those of a commercial cellulase. fermentatio ... | 2011 | 21837419 |
| role of the pyridine nitrogen in pyridoxal 5'-phosphate catalysis: activity of three classes of plp enzymes reconstituted with deazapyridoxal 5'-phosphate. | pyridoxal 5'-phosphate (plp; vitamin b(6))-catalyzed reactions have been well studied, both on enzymes and in solution, due to the variety of important reactions this cofactor catalyzes in nitrogen metabolism. three functional groups are central to plp catalysis: the c4' aldehyde, the o3' phenol, and the n1 pyridine nitrogen. in the literature, the pyridine nitrogen has traditionally been assumed to be protonated in enzyme active sites, with the protonated pyridine ring providing resonance stabi ... | 2011 | 21827189 |
| transformation of chenodeoxycholic acid by thermophilic geobacillus stearothermophilus. | we performed a series of experiments with geobacillus stearothermophilus, a thermophile isolated from oil-contaminated soil in the kuwaiti desert. the organism has a good potential for the transformation of a broad spectrum of organic molecules such as steroids, amino acids, and aromatic hydrocarbons. in the present study, we tested its potential for the transformation of a bile component, chenodeoxycholic acid (cdca). five transformed products, namely, cholic acid, methylcholate, methylchenodeo ... | 2011 | 21838799 |
| toward the smallest active subdomain of a tim-barrel fold: insights from a truncated +¦-amylase. | amytm is a truncated mutant of the +¦-amylase of bacillus stearothermophilus us100. it has been derived from the wild type amylase gene via a reading frame shift, following a tandem duplication of the mutant primer, associated to an adenine base deletion. amytm was composed of 720 nucleotides encoding 240 amino acid residues out of 549 of the wild type. the amytm protein was devoided of the three catalytic residues but still retains catalytic activity. it is ca-independent maltotetraose producin ... | 2011 | 21741359 |
| importance of the c2, n7, and c8 positions to the mutagenic potential of 8-oxo-2'-deoxyguanosine with two a family polymerases. | 8-oxo-2'-deoxyguanosine (odg) is a prominent dna lesion produced from the reaction of 2'-deoxyguanosine (dg) with reactive oxygen species. while dg directs the insertion of only dctp during replication, odg can direct the insertion of either dctp or datp, allowing for the production of dg → dt transversions. when replicated by klenow fragment-exo (kf-exo), odg preferentially directs the incorporation of dctp over datp, thus decreasing its mutagenic potential. however, when replicated by a highly ... | 2011 | 22081979 |
| comparative hydrogen-deuterium exchange for a mesophilic vs thermophilic dihydrofolate reductase at 25 °c: identification of a single active site region with enhanced flexibility in the mesophilic protein. | the technique of hydrogen-deuterium exchange coupled to mass spectrometry (hdx-ms) has been applied to a mesophilic (e. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (b. stearothermophilus) ortholog, ec-dhfr and bs-dhfr, respectively. the analysis of hydrogen-deuterium exchange patterns within proteolytically derived peptides allows spatial resolution, while requiring a series of controls to compare orthologous proteins with only ca. 40% sequence i ... | 2011 | 21859100 |
| cloning and sequence analysis of novel dna polymerases from thermophilic geobacillus species isolated from hot springs in turkey: characterization of a dna polymerase i from geobacillus kaue strain nb. | the complete coding sequences of the pola genes from seven thermophilic geobacillus species, isolated from hot springs of gönen and hisaralan in turkey, were cloned and sequenced. the pola genes of these geobacillus species contain a long open reading frame of 2,637 bp encoding dna polymerase i with a calculated molecular mass of 99 kda. amino acid sequences of these geobacillus dna polymerases are closely related. the multiple sequence alignments show all include the conserved amino acids in th ... | 2011 | 21870126 |
| mutations altering the interplay between gkdnac helicase and dna reveal an insight into helicase unwinding. | replicative helicases are essential molecular machines that utilize energy derived from ntp hydrolysis to move along nucleic acids and to unwind double-stranded dna (dsdna). our earlier crystal structure of the hexameric helicase from geobacillus kaustophilus hta426 (gkdnac) in complex with single-stranded dna (ssdna) suggested several key residues responsible for dna binding that likely play a role in dna translocation during the unwinding process. here, we demonstrated that the unwinding activ ... | 2011 | 22174946 |
| Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans and proposal of Anoxybacillus caldiproteolyticus sp. nov. | Sixty-two strains of thermophilic aerobic endospore-forming bacteria were subjected to polyphasic taxonomic study including 16S rRNA gene sequence analysis, polar lipid and fatty acid analysis, phenotypic characterization, and DNA-DNA hybridization experiments. Distinct clusters of the species Geobacillus stearothermophilus, Geobacillus thermodenitrificans, Geobacillus toebii and Geobacillus thermoglucosidasius were formed, allowing their descriptions to be emended, and the distinctnesses of the ... | 2011 | 21856988 |
| How to change the oligomeric state of a circular protein assembly: switch from 11-subunit to 12-subunit TRAP suggests a general mechanism. | Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements. The subunit number is arguably the most critical parameter of a circular protein assembly, affecting the internal and external diameters of the assembly and often impacting on the protein's function. Although accurate structural information has been obtained for several circular proteins, a lack of accurate information on alternative oligo ... | 2011 | 21984911 |
| Presence and potential role of thermophilic bacteria in temperate terrestrial environments. | Organic sulfur and nitrogen are major reservoirs of these elements in terrestrial systems, although their cycling remains to be fully understood. Both sulfur and nitrogen mineralization are directly related to microbial metabolism. Mesophiles and thermophiles were isolated from temperate environments. Thermophilic isolates were classified within the Firmicutes, belonging to the Geobacillus, Brevibacillus, and Ureibacillus genera, and showed optimum growth temperatures between 50°C and 60°C. Sulf ... | 2012 | 22159635 |
| Isolation and characterization of N-acylhomoserine lactonase from the thermophilic bacterium, Geobacillus caldoxylosilyticus YS-8. | Geobacillus caldoxylosilyticus YS-8, which was isolated from volcanic soil in Indonesia, was found to degrade various N-acylhomoserine lactones (AHLs) with different lengths and acyl side-chain substitutions over a wide temperature range of 30-70 °C. The purified AHL-degrading enzyme showed a single band of 32 kDa, and its N-terminal amino acid sequence was determined to be ANVIKARPKLYVMDN, tentatively suggesting that the AHL-degrading enzyme was AHL lactonase. The AHL-degrading activity of the ... | 2011 | 21897031 |
| reactivation of a thermostable lipase by solid phase unfolding/refolding effect of cysteine residues on refolding efficiency. | lipase from geobacillus thermocatenulatus (btl2) was immobilized in two different matrixes. in one derivative, the enzyme was immobilized on agarose activated with cyanogen bromide (cnbr-btl2) via its most reactive superficial amino group, whereas the other derivative was covalently immobilized on glyoxyl agarose supports (gx-btl2). the latter immobilization protocol leads to intense multipoint covalent attachment between the lysine richest region of enzyme and the glyoxyl groups on the support ... | 2011 | 22112565 |
| novel thermostable and alkalitolerant amylase production by geobacillus stearothermophilus hp 3. | this study reports the production and characterisation of a novel thermostable alkaline amylase from the newly isolated geobacillus stearothermophilus hp 3. the optimum temperature and ph for enzyme production was 55°c and 9.0, respectively. the amylase powder obtained from the culture filtrate by pre-chilled acetone treatment was stable over a wide ph range and liquefied thick starch slurries at 75°c. ca(2+) and co(2+) were required for the thermostability of the enzyme preparation. the present ... | 2011 | 22122512 |
| Gating NO Release from Nitric Oxide Synthase. | We have investigated the kinetics of NO escape from Geobacillus stearothermophilus nitric oxide synthase (gsNOS). Previous work indicated that NO release was gated at position 223 in mammalian enzymes; our kinetics experiments include mutants at that position along with measurements on the wild type enzyme. Employing stopped-flow UV-vis methods, reactions were triggered by mixing a reduced enzyme/N-hydroxy-l-arginine complex with an aerated buffer solution. NO release kinetics were obtained for ... | 2011 | 22148177 |
| nonclassical protein secretion by bacillus subtilis in the stationary phase is not due to cell lysis. | the carboxylesterase est55 has been cloned and expressed in bacillus subtilis strains. est55, which lacks a classical, cleavable n-terminal signal sequence, was found to be secreted during the stationary phase of growth such that there is more est55 in the medium than inside the cells. several cytoplasmic proteins were also secreted in large amounts during late stationary phase, indicating that secretion in b. subtilis is not unique to est55. these proteins, which all have defined cytoplasmic fu ... | 2011 | 21856851 |
| [detection and transcription of n-alkane biodegradation genes (alkb) in the genome of a hydrocarbon-oxidizing bacterium geobacillus subterraneus k]. | | 2011 | 22168011 |
| creation of metal-independent hyperthermophilic l-arabinose isomerase by homologous recombination. | hyperthermophilic l-arabinose isomerases (ais) are useful in the commercial production of d-tagatose as a low-calorie bulk sweetener. their catalysis and thermostability are highly dependent on metals, which is a major drawback in food applications. to study the role of metal ions in the thermostability and catalysis of hyperthermophilic ai, four enzyme chimeras were generated by pcr-based hybridization to replace the variable n- and c-terminal regions of hyperthermophilic thermotoga maritima ... | 2011 | 22103589 |
| reclassification of bacillus beijingensis and bacillus ginsengi qiu et al., 2009 as bhargavaea beijingensis comb. nov. and bhargavaea ginsengi comb. nov. and emended description of the genus bhargavaea. | we have carried out a polyphasic taxonomic characterization of validly published type strains of species, bacillus beijingensis dsm 19037t and bacillus ginsengi dsm 19038t that were phylogenetically closely related to bhargavaea cecembensis lmg 24411t. all the strains are gram-positive, non-motile, moderately halotolerant and non-spore forming. 16s rrna gene sequence analyses showed that these constituted a coherent cluster, with sequence similarities between 99.7 and 98.7 %. the percentage simi ... | 2011 | 22155760 |
| purification and characterization of intracellular and extracellular α-glucosidases from geobacillus toebii strain e134. | two different α-glucosidase-producing thermophilic e134 strains were isolated from a hot spring in kozakli, turkey. based on the phenotypic, phylogenetic and chemotaxonomic evidence, the strain was proposed to be a species of g. toebii. its thermostable exo-α-1,4-glucosidases also were characterized and compared, which were purified from the intracellular and extracellular fractions with estimated molecular weights of 65 and 45 kda. the intracellular and extracellular α-glucosidases showed optim ... | 2011 | 22028281 |
| Development of a fluorescently labeled thermostable DHFR for studying conformational changes associated with inhibitor binding. | A fluorescently-labeled, conformationally-sensitive Bacillus stearothermophilus (Bs) dihydrofolate reductase (DHFR) (C73A/S131C(MDCC) DHFR) was developed and used to investigate kinetics and protein conformational motions associated with methotrexate (MTX) binding. This construct bears a covalently-attached fluorophore, N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC) attached at a distal cysteine, introduced by mutagenesis. The probe is sensitive to the local molecular en ... | 2011 | 21907182 |
| organization and evolution of the cotg and coth genes of bacillus subtilis. | the cotg and coth genes of bacillus subtilis encode two previously characterized spore coat proteins. the two genes are adjacent on the chromosome and divergently transcribed by σ(k), a sporulation-specific σ factor of the rna polymerase. we report evidence that the coth promoter maps 812 bp upstream of the beginning of its coding region and that the divergent cotg gene is entirely contained between the promoter and the coding part of coth. a bioinformatic analysis of all entirely sequenced prok ... | 2011 | 21984783 |
| Meticillin-resistant Staphylococcus aureus is more resistant to vaporized hydrogen peroxide than commercial Geobacillus stearothermophilus biological indicators. | Vaporized hydrogen peroxide (VHP) is increasingly used in the decontamination of hospital isolation rooms. Commercially available bioindicators, most frequently Geobacillus stearothermophilus spores, are used to assess the efficacy of the decontamination phase. Staphylococcus aureus, including meticillin-resistant S. aureus (MRSA), produce catalase, which breaks down VHP, therefore potentially making it resistant to the decontamination phase. | 2012 | 22137066 |
| relevant factors affecting microbial surface decontamination by pulsed light. | pulsed light (pl) uses intense flashes of white light rich in ultraviolet (uv) light for decontamination. a log-reduction higher than 5 was obtained in one flash and at fluences lower than 1.8j/cm(2) on spores of a range of spore-forming bacteria, of vegetative cells of non-spore-forming bacteria and on yeasts spread on agar media. vegetative cells were more sensitive than spores. the inactivation by pl of bacillus subtilis, b. atrophaeus, b. cereus, geobacillus stearothermophilus, and aspergill ... | 2012 | 21924512 |
| A commensal symbiotic interrelationship for the growth of Symbiobacterium toebii with its partner bacterium, Geobacillus toebii. | ABSTRACT: | 2011 | 22023733 |
| proteomics analysis of aromatic catabolic pathways in thermophilic geobacillus thermodenitrificans ng80-2. | geobacillus thermodenitrificans ng80-2 is a crude oil-degrading thermophilic bacterium isolated from an oil reservoir in china. in this study, the gene clusters and pathways for the degradation of benzoate (via benzoyl-coa), phenylacetate (via phenylacetyl-coa), 4-hydroxyphenylacetate (via 3,4-dihydroxyphenylacetate) and anthranilate (via 3-hydroxyanthranilate) were confirmed using combined in silico analysis and proteomics approaches. it was observed that synthesis of the enzymes catalyzing ini ... | 2011 | 22100410 |
| tryptophan oxidative metabolism catalyzed by geobacillus stearothermophilus: a thermophile isolated from kuwait soil contaminated with petroleum hydrocarbons. | tryptophan metabolism has been extensively studied in humans as well as in soil. its metabolism takes place mainly through kynurenine pathway yielding hydroxylated, deaminated and many other products of physiological significance. however, tryptophan metabolism has not been studied in an isolated thermophilic bacterium. geobacillus stearothermophilus is a local thermophile isolated from kuwait desert soil contaminated with petroleum hydrocarbons. the bacterium grows well at 65 °c in 0.05 m phosp ... | 2011 | 22084599 |
| a new family of carbohydrate esterases is represented by a gdsl hydrolase/acetylxylan esterase from geobacillus stearothermophilus. | acetylxylan esterases hydrolyze the ester linkages of acetyl groups at positions 2 and/or 3 of the xylose moieties in xylan and play an important role in enhancing the accessibility of xylanases to the xylan backbone. the hemicellulolytic system of the thermophilic bacterium geobacillus stearothermophilus t-6 comprises a putative acetylxylan esterase gene, axe2. the gene product belongs to the gdsl hydrolase family and does not share sequence homology with any of the carbohydrate esterases in th ... | 2011 | 21994937 |
| cloning, expression and applicability of thermo-alkali-stable xylanase of geobacillus thermoleovorans in generating xylooligosaccharides from agro-residues. | a xylanase gene (xyl-gt) of 1.224kbp was cloned from the extremely thermophilic bacterium geobacillus thermoleovorans that encodes a protein containing 408 amino acid residues. eight conserved regions (signature sequences) of gh family 10 xylanases have been found in the xylanase. when the xylanase gene was cloned and expressed in escherichia coli bl21 (de3), the recombinant strain produced xylanase titer of 270umg(-1) which is 27-fold higher than the wild strain. it is optimally active at 80°c ... | 2011 | 22212694 |
| crystal structure of quinol-dependent nitric oxide reductase from geobacillus stearothermophilus. | the structure of quinol-dependent nitric oxide reductase (qnor) from g. stearothermophilus, which catalyzes the reduction of no to produce the major ozone-depleting gas n(2)o, has been characterized at 2.5 å resolution. the overall fold of qnor is similar to that of cytochrome c-dependent nor (cnor), and some structural features that are characteristic of cnor, such as the calcium binding site and hydrophilic cytochrome c domain, are observed in qnor, even though it harbors no heme c. in contras ... | 2012 | 22266822 |